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PMC11711871
Differences were assessed at *P < 0.05, **P < 0.01, and ***P < 0.001 Increased inactive plasma metabolites of the linoleic, eicosapentaenoic, and docosahexaenoic acid pathways in GR rats under high-salt diet.
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PMC9429973
In hit validation experiments, knockout of NPM1 abrogated in vivo growth in all PDX AML models tested, reproducing the known common essential function of NPM1.
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PMC11422497
All pathological specimens were confirmed by experienced pathologists and did not undergo pre-operative radiotherapy, chemotherapy, or imatinib targeted therapy.
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PMC10165258
By mixing the dyes with the stock drug solutions, the concentration of the dye could be used as a measure of the drug concentration for the different dilutions.
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PMC11407042
The authors declare no competing interests.
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PMC11036064
In addition, the combined treatment has considerably decreased CDK4, CDK6, CCND1, and MMP2 compared to monotherapy with temozolomide in A172 cells.
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PMC11727907
For both assays, 20 μg of total protein was utilized.
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PMC7519871
These results support the idea that sequential combination of cisplatin with WDL and BA may work effectively in cervical cancer cells.
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PMC11627398
Based on the data from the GSE57065 cohort, differential expressed miRNAs (DEmiRNAs) and differential expressed lncRNAs (DElncRNAs) were identified between control and septic shock groups.
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PMC9429973
Subjects received treatment with tolinapant at the RP2D 180 mg/day on Days 1 to 7, and 15 to 22 in a 28-day cycle.
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PMC10940855
Deidentified, healthy control BM samples were obtained from AllCells.
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PMC7053076
The data were visualized using Flexstation 3.
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PMC11748618
Previously, we tested the association of individual CHD1L eQTLs from the AFGR cohort against the effect of HIV spVL from the ICGH HIV spVL cohort.
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PMC11411004
Epigenetic silencing is a potential limiting factor in the efficient and stable production of recombinant proteins in rCHO cells (Jia et al. 2018).
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PMC5034105
A virus' ability to grow in cells of different host species might provide insight into its ability to cross interspecies barriers.
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PMC11657744
A two-way ANOVA test followed by Tukey’s multiple comparisons was performed. *,
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PMC11551844
The percentage yield of AuNPs-JDH, AuNPs-JDE and AuNPs-JC-2—assuming full conversion of the initial mass of JDH, JDE and JC-2—with respect to HAuCl4·3H2O was 69.7%, 77.7% and 54.5%.
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PMC6461034
Phosphosite data: the MaxQuant “Phospho (STY)Sites” table is processed: Rows are filtered for so‐called class I sites (localization probability > 0.75).
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PMC10818573
Moreover, more possibilities for rapid the binding of target fragments in this amplification system can be explored to reduce the time required to extract DNA and simplify the overall process.
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PMC11499955
In this paper we described a multiplexed analysis diagnostic approach based on cleavable MS-tagged antibodies.
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PMC11705862
This is due the fact that we have limited access to tissue post-fixed according to the specific protocols we use for immunostainings.
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PMC11473750
3RNF121 is necessary for neuroblastoma development in TH-MYCN mice.mRNA expression level of a RNF121, and, b a MYC gene signature in ganglia tissue and tumours from wild-type (WT) and TH-MYCN mice, obtained at different postnatal ages (1, 2, and 6 weeks of age; n = 4 per group).
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PMC11499381
Cells were stimulated with αCGRP with duplicate or triplicate technical replicates.
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PMC11650848
While numbers in the current RCT were small and require further validation in larger cohorts, the results nevertheless suggest a molecular mechanism by which maternal folic acid supplementation during pregnancy may help to counteract the effects of folate depletion and positively influence cognitive development in the offspring.
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PMC11453690
Adipocyte diameter was measured in hematoxylin and eosin (H&E) stained sections using ImageJ. Results: Aromatase mRNA levels were correlated with BMI and were higher in postmenopausal women (p≤0.05), with the standard decantation group demonstrating a stronger correlation with BMI.
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PMC9429973
S.-Y. Liu, C.-Y. Yuan, X.-H. Sui, X. Liu, Y. Li, N.-N. Shan Department of Hematology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan; Department of Hematology, Dezhou Hospital Affiliated to Qilu Hospital of Shandong University, Dezhou; Department of Hematology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China Background: Immune thrombocytopenia (ITP) is an acquired autoimmune disease characterized by isolated low platelet counts due to platelet autoimmune destruction and megakaryocytogenesis defects.
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PMC9412887
Hyperthermia, a non-invasive treatment strategy, has shown a competitive advantage in reversing drug resistance in cancer by suppressing the expression of drug efflux transporters .
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PMC10079526
Mice were treated with 5-Aza-2’ and/or TAK981, which continued bi-weekly for 31 days after tumor engraftment (Fig. 5A).
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PMC11237030
We also observed a residual expression of AQP5 in the cell culture supernatant for the clone 16, probably derived from dying cells releasing the protein extracellularly and the presence of higher bands in the range of 100 KDa corresponding likely to tetramers, trimers or dimers form of AQP5 protein which are resistant to SDS.Figure 4AQP5 protein expression and plasma membrane localization. (
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PMC11766174
To further test the applicability of CA-ICC to a non-human primate model, PBMCs longitudinally isolated from two individuals before SIV infection (day −11), after SIV infection (day 14), and after initiation of antiretroviral treatment (day 84) were evaluated.
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PMC11686380
Brain immunostaining with anti-myc antibody at postnatal day 0 (P0) showed significant expression of myc-GAP43 (WT) in layer II/III neurons.
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PMC11320084
b, c) TNNT1 expression in LC cells was measured by quantitative real‐time‐polymerase chain reaction (qRT‐PCR) and western blot assays. (
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PMC10329237
Following the migration, transcription factors are activated, working as repressors of E-cadherin; thus epithelial cells become motile (46).
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PMC11802929
These pairs including CCR7 and CD197-CCR7-CITEseq, IL7R and CD127-IL-7RA-CITEseq.
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PMC10969097
The solvent from the filtered extract solution was evaporated using a rotary evaporator at 35–40 °C and 200–250 rpm to remove the methanol and ethanol.
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PMC11638288
The study by Teppei et al. demonstrated that the cytolytic activity of NK cells co-cultured with hepatocellular carcinoma cells transfected with HBV genome head-to-tail dimers was higher compared to that of NK cells co-cultured with non-transfected hepatocellular carcinoma cells.
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PMC10092581
These were synthesized in accordance with our previous reports (see Supporting Information).[ , , ]
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PMC11352746
Thus, screening psychoplastogenic molecules in stem cell-derived disease models will be a step in this journey of CNS drug discovery.
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PMC11759751
Our results show that B-NHL cells pretreated with low concentration of chidamide can enhance the expression of CD19 on the cell surface and promote the secretion of cytokines by D19 CAR T cells.
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PMC11476264
Overall, these results indicate that FBXO11 interacts with ZEB1 in vitro.
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PMC9429973
Aims: Our aim was to perform a multi-dimensional analysis of health outcomes in AML long-term survivors (AML-LTS).
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PMC11684821
However, we have not observed such alloreactivity.
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PMC9352806
Some natural functional components act as AMPK activators, like EGCG (Madeo et al., 2019), resveratrol (Milton et al., 2018) and cinnamaldehyde (Neto et al., 2019), can activate the AMPK pathway in liver cells and improve the autophagy level, thereby reducing lipid accumulation.
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PMC11701801
Cells were then reseeded and transfected with the indicated siRNAs for 48 hours.
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PMC11764090
WRKY mainly responds to a variety of induced stimuli by specifically recognizing W-Box and initiating the expression of regulatory genes, thus playing a regulatory role in plants .
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PMC9429973
To discover biomarkers that may help identifying patients that will benefit from checkpoint inhibitor therapy.
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PMC10890307
All cultures were repeated at least twice.
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PMC9429973
In addition, inhibition of CSN5/Jab1 activity by CSN5i-3 resulted in increased IFN-β and IFN-y mRNA levels in ALK+ ALCL cells, and CXCL10, IFN-y and STING mRNA levels in ALK- ALCL cells.
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PMC11650848
average methylation between 6 CpG sites measured by pyrosequencing, SD standard deviation, min minimum, max maximum, N number of samples, significance indicated by p < 0.05 and is measured by ANOVA between the genotypes Methylation quantitative trait loci in the differentially methylated region of ZFP57.
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PMC9784246
Reviewing the literature revealed that most of these metabolites exhibit antileishmanial activity against amastigotes and/or promastigotes forms of one or more Leishmania spp.
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PMC11768927
Silencing CHD1 and MAP3k7 also decreased the constitutive expression of ISGs, emphasizing their role in the retention of an IFN-I response and the subsequent resistance to VSV.
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PMC11509224
Gracilins J–L belong to the rare classes trisnorditerpenes, bisnorditerpenes, and norditerpenes, respectively.
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PMC11707577
The ABMDMA assay determines the singlet oxygen generated during visible light irradiation (type II mechanism) following the decrease of ABMDMA absorbance upon irradiation: the singlet oxygen reacts with ABMDMA to give a non‐absorbing endoperoxide.
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PMC11779605
A replicate of this model was conducted as described for the discovery model, with the exception that 10 million ES-2 cells were injected.
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PMC11730000
Informed Consent: Not required.
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PMC11496728
Apoptotic protease activating factor 1 (Apaf1) is a human homolog of Caenorhabditis elegans CED-4, suggesting ways to identify CED-4 and its underlying genes.
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PMC9429973
Methods: Patients who completed the main study enrolled in the open‐label extension study and continued receiving 25-50 IU/kg SCT800 every other day or three times weekly.
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PMC11541409
This reference is of importance because clearly presents an example of covalent-binding TKI to better inhibit the oncogenic pathways in cytosolic-kinase-mutated cancers than the non-covalent binding TKI in clinical setting.
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PMC9429973
Summary/Conclusion: Parsaclisib in combination with bendamustine + obinutuzumab appears to have a manageable safety profile and demonstrated promising efficacy in pts with R/R FL.
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PMC9429973
Results: Median age was 58 years (range; 51 to 65 years).
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PMC10932641
This moderate sensitivity implies that using MALAT-1 as a blood-based prostate cancer biomarker may lead to a large number of false-negative results, possibly due to some degree of degradation of MALAT-1 in the blood (51).
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PMC11736898
Lower levels of staining were observed in normal skin (Figure 4g–i).
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PMC11518702
The construction of mice expressing HGAL with a mutated PBM would provide in vivo insight into HGAL-mediated PDZ-RhoGEF signaling.
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PMC7185206
Metalloprotease inhibitors have shown promise in preventing post-radiation resistance in non-small cell lung cancer and in treating breast cancer.
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PMC8041314
To address the second question as indicated above, a list of in total 308 partial structures was compiled that are commonly present in organic molecules (names and SMILES codes can be found in Supplementary Table 4).
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PMC9019892
GIST‐T1, KIT‐T670I/BaF3, GIST‐T1‐T670I and GIST‐5R mouse xenograft models were used to assess the in vivo antitumour efficacy of nintedanib.
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PMC11297139
Disruption of ARID1A LLPS in a cancer cell line weakened the effects of Ewing’s sarcoma’s oncogenic potential, indicating that the condensates initiated by ARID1A play a role in the cancer phenotype.
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PMC9429973
Additional analyses, including ctDNA, are ongoing and updated data will be presented.
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PMC9429973
Aims: Primary aim was to evaluate the prevalence of mutant C677T MTHFR and its association with thrombosis in the Greek paediatric population.
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PMC6160470
One-way ANOVA with Tukey’s post-hoc test was used for statistical analysis (NS – non-significant; *p < 0.05).
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PMC11173119
The results showed a complete match of 25 loci from both samples (data available on request from the authors).
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PMC11286266
Subsequently, 2K cells were targeted to generate 2K-CRT∆ derivatives cells, enabling a comparison of ATF6α processing.
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PMC3675084
Mitotic catastrophe occurs during or shortly after a deregulated or failed mitosis and can be accompanied by morphological alteration such as micronucleation (the appearance of chromosomes or chromosomal material outside of the two daughter nuclei) and multinucleation (the appearance of two or more nuclei similar or heterogeneous in size, resulting from deficient separation during cytokinesis) , , .
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PMC11675244
Among all the proteins included in this degradation assembly, namely APC, Axin, GSK-3β, and CK1 have a pivotal role in blocking excessive Wnt signaling by facilitating the binding between β-catenin to the rest of the proteins in the complex .
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PMC11547972
A toxic or lethal HCN-concentration following amygdalin consumption has not been established.
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PMC11627398
Additionally, SLC31A1 and MTF1 showed a positive correlation with natural killer cells and LIAS and LIPT1 exhibited a positive correlation with CD8+ T cells.
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PMC11604830
In the in vitro experiments performed the highest SNR value was observed for the PFCE‐NE sample, probably due to its higher proton concentration.
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PMC11700165
This observation underlines the uniqueness of transforming events.
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PMC11786600
Immunofluorescence microscopy images of MMP9 expression. (
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PMC8382973
A decrease in cell viability greater than 30% is considered cytotoxic.
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PMC11789597
The ROS level indicated by CellROX Deep Red dye or mitoSOX Red in living (SYTOX Blue negative) cells was determined using the CytoFLEX flow cytometry and analyzed using FlowJo software.
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PMC10253973
Eilatin is an alkaloid derived from the marine tunicate Eudiastoma sp. The anti-HIV activity against HeLa cells infected with HIV-1 was estimated with the use of a plaque-formation assay.
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PMC8992508
These three proteins, as it is for most ABC transporters, are composed of two domains: the transmembrane domain (TMD) and the nucleotide binding domain (NBD), which binds ATP.
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PMC11544771
However, cleaved caspase 9, another initiator caspase, was not altered in the cells (Figure 5D,E and Figure S3A,B).
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PMC8913139
no. 58802; Abcam) for 1 h. Finally, protein bands were assessed by ECL kit (Beyotime).
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PMC11322866
Total protein lysates of RPMI-8226 and OCI-MY5 cells were collected for co-immunoprecipitation using anti-RRM2 and anti-EZH2 antibodies. (
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PMC11747949
143B cells secrete CCL2, more in normoxia than in hypoxia, but it can acutely increase by IFNγ stimulation (Figure 1B).
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PMC9545714
These fatty acids are antimicrobials.
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PMC11365427
Flow cytometry shows the percent of apoptotic cells in NCI-H929 and RPMI-8226 cells after 48 h of phenylalanine deprivation combined with Z-VAD-FMK (50 μmol/L). (
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PMC8358006
HEK293T cells were cultured in DMEM with 10% FBS.
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PMC11095939
EV, empty vector.
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PMC11543885
Fixed Modification: Set Carbamidomethyl (C) as the fixed modification.
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PMC7081114
Silencing KIF21B significantly decreased the total number of cells and the rate of cell growth (Figure 3A-B).
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PMC9429973
Adding GO to FLAI did not improve MRD negativity probability.
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PMC9847912
As a result, the formation of a complex in which GSK232 locks CA-SP1 in the stable 6-HB structure does not occur, and HIV-2/SIV maturation proceeds unabated.
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PMC11597167
Different studies may employ different protocols, leading to variations in results.
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PMC11052306
These combinations were tested against two cancerous cell lines, MCF-7 and HepG2 (human liver carcinoma).
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PMC10142392
However, the choice of platform will depend on the specific characteristics of the disease, underlying biological mechanisms, potential routes of administration, patient compliance, and other factors.
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PMC11420784
We speculate that UPEC PldA employs a strategy similar to the use of PlcA/B in Listeria.
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PMC7582629
After 6 MP treatment, B-ALL cells showed lower intensities of the Raman bands (nucleic acids, phenylalanine and proteins) than MTX-treated cells.
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