PMCID
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PMC11748335
Hence, our results elucidate that tumor cell lines predominantly acquire H3K27me3 long LOCKs within L-PMDs, with a subset originating from regions previously marked by H3K9me3 modification in normal cells.
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PMC11792740
These abnormalities in TCR signaling affect T-cell activation, proliferation, and differentiation, leading to diminished antitumor capabilities.
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PMC7039683
SNP1: rs11170342, SNP2: rs2070875, SNP3: rs3741442, SNP4: rs11170344, SNP5: rs7299694, SNP6: rs6580920, SNP7: rs4503623, SNP8: rs2363635, SNP9: rs2682339, SNP10: rs111680692, SNP11: rs2363632, SNP12: rs4919749, SNP13: rs2638522, SNP14: rs9634243.
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PMC5839394
SKBR3 cells were seeded on 24-well tissue culture plates and grown to 100% confluence.
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PMC5600151
Overall, G3-G10 PAMAM dendrimers form more stable dendriplexes with nucleic acid molecules.
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PMC9429973
Methods: PERSIST-2 enrolled patients with platelet counts ≤100 x 10/L and randomized them 1:1:1 to pacritinib 200 mg twice daily (BID), pacritinib 400 mg once daily (QD), or best available therapy (BAT).
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PMC11708541
A cyclization of the derivative of carbamothioyl methacrylamide into substituted 1,3-thiazine was demonstrated.
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PMC11206251
The apoptotic effect of the PEIM was dose-dependent.
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PMC11467964
D and E) The waterfall plot of somatic mutation features established with high and low risk scores. (
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PMC9429973
Combining data of CIRS≥6 and low-SMI, 40 (34%) patients, 47 (40%) and 31 (26%) displayed 0, 1 and 2 markers (Fig. 1D).
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PMC11621565
Although permeabilization enables the interaction between microbiota compounds and other cells in the intestinal wall, such as neurones from the enteric plexus, it also promotes the transport of microbiota metabolites (including LPS) into the bloodstream.
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PMC7039683
Therefore we predicted that, similarly, upon training a classifier on zGPAEs, mPEAEs, or hOEAEs, deltaSVM scores of SNPs within known epithelium enhancers would correlate with the differences in reporter activity between risk and non-risk constructs tested in a human basal oral keratinocyte cell line.
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PMC11269933
The clean reads of RNA-Seq were aligned to the oyster genome (GenBank accession no. GCA_011032805.1) using HISAT2 v2.1.0.
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PMC9429973
Prior therapy with venetoclax is permitted.
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PMC11496728
Fig. 6Comparison of transient expression of eGFP in CHO-WT and CHO-KO cells.
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PMC11627133
SiR lights up upon binding to the aptamer, SiRA.
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PMC11682172
Cells were cultured in RPMI-Hepes (Gibco) with 1% l-glutamine (BioWhittaker), 1% P/S and 6% human serum albumin (HSA), in combination with phytohemagglutinin-L (PHA-L, Sigma) and IL-2 in a 96-well flat-bottomed plate for 6–7 days at 37 °C, in a humidified incubator with 5% CO2.
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PMC10994876
Previous studies have shown that c-Myc positively regulates the transcription of Cdc25A, which serves as a positive regulator of cdk2.
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PMC9429973
Summary/Conclusion: In the early stage of transplantation,patients with multiple stable symptom cluster and sykptoms severity score were negatively correlated with QOL score.medical staff should manage the symptoms of patients in the early stage of transplantation,and carry out effective management to improve the QOL of patients.
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PMC11788920
RNP4 was not used in either the wild-type or GQ-Mut constructs, as it did not overlap with the PQS.
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PMC11682224
By triggering the Wnt/β-catenin/TCF pathway, CHD1L influences the proliferation of cells in pancreatic cancer.
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PMC10329237
Image J analysis software was used to measure gap areas and calculate the relative cell migration of the treated to untreated control cells.
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PMC11705484
H, I) Transcript levels of mA modification readers (YTHDF1‐3 and YTHDC1) in human BLCA tissues and adjacent normal tissues in the TCGA database and 24 patients (in‐house clinical cohort); T: bladder cancer tissues; N: adjacent normal tissues. (
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PMC11587606
The various and important functions of RHBDF1 make this protein an attractive subject for cancer research.
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PMC9583612
In addition to improving methane emissions estimates, many measurement technologies can identify and reduce methane emissions in the near term, identifying leaks at the equipment level and acting as event detectors, which will provide operational and climate benefits.
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PMC11696576
A tendency to suppress both 6-keto PGF1α and 12-HETE was also observed although IC50 values could not be calculated (Fig. S1b).
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PMC9429973
PCR outlier results were identified using the ROUT method by nonlinear regression with a maximum false discovery rate (FDR) of 1% (Motulsky et al 2006).
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PMC11544122
BRET was determined from the average of three consecutive reads (a, b) (1–3 min for a, or 9–11 min for b) or six consecutive reads (4–11) (c).
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PMC11535726
In addition, ex-type living cultures were preserved at the Kunming Institute of Botany Culture Collection (KUMCC) and the Guizhou Culture Collection (GZCC).
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PMC11306331
We found that kinetics were only dramatically changed by the transition from whole-cell (τ = 0.32 + /− 0.05 min) to partial-cell illumination (τ = 1.74 + /− 0.27 min), indicating that protein transport in and out of the illumination region plays an important role in the timescale of light-induced condensate disassembly.
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PMC11770130
Consistently, the PERKa treatment normalized the serum level of GDF15 as well as food intake in Ntsr2 AKO mice (Supplementary information, Fig. S8j, k).
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PMC5417661
The following drug concentrations, selected on the basis of a pilot test, were used; NOV202 (10, 50 and 200 nM) and Combretastatin A4 (10 and 50 nM).
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PMC11635519
The central panel zoomed in on the conformations sampled by the N-terminal residue (His1) of PepA. The right panel showed the interactions of the N-terminal residue with the receptor binding site in the most representative cluster. (
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PMC11087055
Thus, the role of the Wnt/PCP signaling pathway for the polonaise movements has remained unresolved.
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PMC10329237
In Rasooli et al.’s study, combination therapy was performed as a new therapeutic strategy for IPF treatment (27).
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PMC9918897
The prevention and treatment of cancer is one of the most important topics in medical research.
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PMC9341513
We note that the probes cover a region several times larger than the CCND1 gene body (42.8 kbp compared to the 13.4-kbp gene), so this is an indirect measure.
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PMC9275501
BA may also inhibit the proliferation, migration, invasion and tumorigenesis of pancreatic cancer cells by activating AMPK signal, and it combined with gemcitabine (GEM) had an antitumor effect on pancreatic cancer cells (Sun et al., 2019).
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PMC11367146
The cell morphology in untreated cells was spherical and regular.
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PMC10507284
Considering that the number of tumor mutation burdens (TMB) and microsatellite instability (MSI) are essential indicators for predicting immune efficacy, we explored the association between TROAP, TMB, and MSI.
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PMC9895440
n = 8 donors of n = 4 experiments.
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PMC9429973
At data cutoff (Oct 15, 2021), median follow-up duration was 10.7 mo (range 0.4–42.8).
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PMC11128598
Data are presented as mean ± standard deviations.
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PMC11795610
Finally, the cells were observed under a fluorescence microscope and five visual fields were randomly chosen to calculate the fluorescence intensity.
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PMC9429973
Our results showed a trend toward a younger age at diagnosis in patients harboring null mutations compared to patients with missense mutations (13 vs 17 years, p=0,086) and more chronic infections during follow-up (74% vs 25%, p<0,001).
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PMC11629192
CSF analysis showed elevated WBC with normal protein, and the anti-NMDAR antibody titer was 1:30.
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PMC9429973
In a humanized murine model of aGvHD and GVL demonstrated that GCs and 3PO cooperatively reduced the alloreactivity of T cells and ameliorate aGvHD without a loss of GVL effects.
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PMC10218459
CPP treatment was performed indirectly by treating 200 µL of full medium with CPP for 5 s in a 24-well plate.
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PMC11366496
As an E3 ubiquitin ligase, how does RNF122 activate JAK2/STAT3 pathway?
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PMC11640555
Since brain cancer is characterized by uncontrolled cell growth, strong evidence demonstrates an elevated level of NFs in GBM, although physiologically they are involved in the induction of neurite outgrowth .
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PMC11380329
The percentage of diverse material present in propolis depends upon the time of its collection and also on the geographical origin .
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PMC7709199
The crystallographic twofold axis present in the I41 lattice is located at Cys197, thereby forming a crystallographic homodimer.
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PMC11738017
The dark red box indicates patients who received TCE and the yellow box indicates patients who received anti-BCMA CAR T. (B) PFS of the patient population in months. (
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PMC10142392
Additionally, PCL exhibits excellent mechanical and physical characteristics, as well as ease of processing and shaping at low temperatures.
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PMC8839885
No increase in HIF1α levels was observed in OC cells co-cultured with MSC (Figure 1C).
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PMC11589157
Subsequently, the DNA ends were labeled with biotin-14-dCTP, and blunt-end ligation of the cross-linked fragments was performed.
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PMC9473868
In this study, we found BAI inhibits FLS proliferation and EMT, whereas it induces cell apoptosis through blocking the PI3K/Akt/mTOR pathway.
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PMC11172027
A significance of p < 0.01 was achieved between the control (100 ± 4.8%) and 125 µg/mL NALM (58.9 ± 8.4%; n = 3).
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PMC7351993
Scale bar, 10 µm.
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PMC10154881
E.G‐7/N4 showed lower cell viability at 1 dpi compared with E.G‐7 (p < 0.01), and a 40% reduction at 3 dpi (p < 0.01) (Figure 2A).
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PMC9429973
Results: At baseline, 69 and 68 pts out of 72 receiving IVO+AZA completed the EORTC QLQ-C30 and EQ-5D-5L, respectively, and 66 pts out of 74 receiving placebo+AZA completed both.
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PMC11209164
HSV.n212 was obtained as a generous gift from Dr. Karen Mossman of McMaster University (Hamilton, ON, Canada).
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PMC11486946
In drug discovery, the largest number of target molecules are membrane receptors (>50% of total), such as receptor tyrosine kinases (RTKs), G protein-coupled receptors (GPCRs), and immune receptors, followed by nuclear receptors (<20%).
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PMC9398137
21.
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PMC7553912
The apparent molecular weights are indicated on the right. (
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PMC10170482
We used this methodology for mitochondrial ROS detection, not occasionally.
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PMC9429973
In vivo, ISB 1442 was assessed in a therapeutic model of subcutaneously established Raji tumor xenograft in CB17/SCID mice compared to daratumumab and magrolimab.
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PMC11594806
Using murine models, the team demonstrated that CD73 expression confers resistance to DOX by suppressing the adaptive immune response via activation of the A2A receptor (A2AR).
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PMC7215832
For a fluorescent signal, goat anti-mouse InfraRedDye and goat anti-rabbit InfraRedDye secondary antibodies were used.
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PMC7351993
After the event is detected, a cell ID number is assigned to the event while a time stamp is attached to the ID number.
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PMC10808409
The thermogram of TPGS mixed micelles (O2) shows all endothermic peaks of polymers as shown in the blank TPGS mixed micelles thermogram, but the characteristic peak of FA at 172.35 °C was absent.
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PMC11506670
In a parallel setup, high-content imaging using an IN Cell Analyzer 6000 (GE Healthcare, Marlborough, MA, USA) was employed to capture spheroid images.
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PMC8409415
Silencing cluster of differentiation 70 (CD70) expression was not associated with platinum resistance and proliferation.
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PMC11257203
P < 0.05, **P < 0.01, and ***P < 0.001 indicate significant differences compared to the control group; P < 0.05 and P < 0.01 between the designated groups.
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PMC10452486
Such misfolding of HLA-B27 may contribute to its recognition by both HC10 and W6/32.
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PMC11482552
Furthermore, presently commercially available HPV assays which include an internal human gene control often apply arbitrarily chosen IC cut-offs, in terms of cycle threshold (Ct) values, by the assay manufacturers, which have not undergone more stringent validation, such as those that are presently required for determining HPV cut-offs in terms of clinical sensitivity and specificity.
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PMC9341513
Figure 7, B and D, shows similar results from simulations where U266 epigenomic input data is used with the intact hg19 genome (no insert, as shown in the cartoon).
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PMC10858941
Then, cells were washed with PBS twice and permeabilised and blocked using PBS containing 5% FBS, 5% horse serum (Fisher Scientific, Massachusetts, USA; 26050088), 0.2% glycine, 0.1% Triton X-100 (Merck Sigma-Aldrich, Massachusetts, USA; T8787).
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PMC9429973
We studied pro-inflammatory, TH1, TH2 and TH17 cytokines (IL-2, IL-4, IL-6, IL-10, INFg, TNFa, and IL-17).
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PMC9985266
However, it is not clear whether IL-27 can attenuate PF by inducing autophagy.
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PMC11401358
As shown in Fig. 3A and Fig. S3, SF exhibited high •OH and H2O2 scavenging activity in a concentration-dependent manner.
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PMC4360730
On the ENCODE portal, the metadata is easily available for search queries using a faceted searching system.
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PMC9847912
These re-feeding and re-dosing steps were performed in order to maximize the viability of the CEMss cells throughout entire culture period.
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PMC11127909
It was proven that it could manipulate neuronal and muscle cell apoptosis and differentiation .
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PMC11640247
Specifically, neurons in bin 3 showed the highest GFP expression, followed by those in bin 2 and bin 1, in descending order (Figure 4F).
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PMC9243326
Initially SSRs of 1–6 nucleotide units were identified with the minimum repeat unit number defined as 15 for mono-nucleotides, 6 for di-nucleotides, 5 for tri-nucleotides, and 4 each for tetra-, penta-, and hexa-nucleotides.
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PMC8711270
With these developments in CHO cell glycoengineering, we foresee a further decline in the use of HEK293 as host cells for therapeutic recombinant protein production.
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PMC9895440
e Tumor volume in mm (mean and SEM) of NSG mice treated 3 days post Raji tumor injection by i.v.
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PMC8494202
The expression level of CKAP2L in acute myeloid leukemia (LAML) was significantly lower than that in the normal control group, but expression of CKAP2L in most malignant tumors was abnormally increased, including in glioblastoma (GBM), as shown in Figure 1(a).
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PMC9454178
Imatinib was kindly provided by Novartis Pharmaceuticals (Basel, Switzerland).
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PMC6889484
We postulate that multiple amino acid substitutions in Vif must appear simultaneously to acquire the ability to induce A3G-D128K degradation.
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PMC10654497
C and D) Enrichment analysis of the GO and KEGG pathways. (
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PMC3915447
As the polymer slowly degrades, therapeutic levels of the anti-tumour peptide are maintained for up to 3 months, making the therapy very convenient for patient use (8).
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PMC10222971
These natural products are widespread in nature and are characterized by possessing two carbonyl groups at positions 1,4 on the naphthalene ring, and more rarely at positions 1,2.
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PMC5261804
High-throughput technology for interrupting specific PPIs on a whole-interactome scale does not exist, and methods for experimentally measuring the essentiality of individual endogenous PPIs at the genome scale will likely remain an unsolved problem for the foreseeable future.
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PMC11438317
These assays collectively revealed that LYP also regulates proteins downstream of LCK and Zap70.Fig.
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PMC9429973
M. Ababou, M. Meziane, C. Chouikh, M. Ababou, J. Laoutid, K. Doghmi Clinical Hematology; Anesthesia and Intensive Care Unit, Military hospital Mohammed v, Rabat, Morocco, Rabat; Anesthesia and Intensive Care Unit, Military Hospital Moulay Ismail, Meknes, Morocco Background: In December 2019, Wuhan City became the center of a pneumonia epidemic caused by a virus of the Coronaviridae family, SARS-CoV-2, which was later designated by the WHO as COVID-19 (Corona Virus Disease-2019).
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PMC11757230
The fact that proliferation recovered or was promoted after separation from cADSCs suggests that the increase in S-phase or G2/M-phase cells was not due to cell cycle arrest at these stages, but rather an accelerated progression from the G1 to S phase.
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PMC11335267
Indeed, H-MRS showed a higher proportion of total lipids associated with Alx3 deficiency (Fig. 1A and B).
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PMC8592717
The level of plasma ET-1, sP-sel, and hs-CRP following treatment with DHI indicated that it could inhibit platelet activation and inflammation, and angiogenesis and protect the endothelial function in patients with acute coronary syndrome (ACS) after PCI [194-198].
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