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PMC9429973
Moreover, two T315I clones were sensitive to venetoclax, but other T315I clones with mutations in other cancer related genes showed insensitivity (n=2) or decreased sensitivity (n=1).
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PMC11453018
While we utilized the pharmacological compound iP300w in this study, similar effects could potentially be achieved with other recently discovered P300/CBP inhibitors such as A-485, CCS1477, NEO2734, and B026 which have shown effectiveness against various malignancies, with some even undergoing clinical trials [19, 68–70].
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PMC11047729
Its anti-leukemic properties were studied on cell line HL-60 and resulted in inhibited proliferation and induced apoptosis.
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PMC11477621
C NMR (125 MHz, CDCl3) δ 188.3, 174.0, 130.3, 130.2, 120.4, 108.0, 60.3, 49.9, 34.5, 31.5, 29.2, 29.0, 27.5, 26.6, 25.0, 14.4.
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PMC11066331
However, the biotyping of eukaryotic cells may represent a technological limitation due to the inherent complexity of samples, requiring the development and/or optimization of protocols tailored to individual experiments or applications.
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PMC9429973
The median number of prior treatment lines was 4 (IQR 3-5).
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PMC9351137
These experiments were performed by transfecting HeLa cells with specific siRNAs against the transcripts encoding the following CRS: BAF53a, CFDP1, GAS41, MRG15, p400, Tip60, and YL1.
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PMC11665555
We found that OXCT1 expression negatively correlated with survival of patients with BCa, both in terms of tumor stage and response to therapeutic intervention (Figure 1 and Supplemental Figure 1).
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PMC9429973
Data will be analysed to answer 3 initial themes (Image 1), and extended further to include control data for Health Technology Appraisals.
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PMC11737091
p < 0.05; **, p < 0.01; ***, p < 0.001 Analysis of the prognostic and diagnostic value of DBF4. (
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PMC8806312
The total points of this nomogram were summed and subsequently converted to reveal the probability of 1-year, 2-year and 3-year survival (Figure 5(b)).
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PMC11380454
Collectively, these data suggest that SAS prevents PD-L1-induced resistance and proliferation in VLA-4-positive malignant cells, likely through the inhibition of PD-L1 expression.
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PMC11680982
The sections were dewaxed in xylene twice for 10 min each and rehydrated with a graded series of ethanol.
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PMC4395774
The cells were cultured in RPMI medium with 10% fetal calf serum, 1% Pen/Strep, 1 mmol/l 2-mercaptoethanol (Invitrogen), 1 mmol/l sodium pyruvate (Invitrogen), and 1000 U/ml IL-2 (Peprotech, Rocky Hill, NJ).
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PMC11541409
Unlike traditional KIT/PDGFRA mutant targets, selinexor targets the XPO1-mediated nuclear export of RNA.
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PMC9429973
Aims: To study the relationship of BCL-2 expression with the response to frontline therapy in nodular sclerosis classical Hodgkin lymphoma (NSCHL).
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PMC11766309
The expression of immune-stimulating genes was positively correlated with YKT6 in the majority of the tumors, especially in OV, UVM, LIHC, READ, and BLCA.
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PMC11687167
PCSK9 regulates the concentration of sirtuins, a family of proteins involved in histone deacetylation, and plays a key role in metabolically driven inflammation .
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PMC9429973
Bilateral involvement at diagnosis was associated with significantly inferior PFS (P=0.029), whereas age, IPI score, or TNM staging was not associated with PFS.
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PMC11144382
Western blots analysis using anti-Flag primary antibodies and HRP conjugated secondary antibody, and (D) quantified by densitometry analysis.
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PMC9429973
Proprietary multiple instance learning (MIL) algorithms and training scheme, based on cases ranking, rather than absolute values, were applied.
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PMC10654497
In contrast, patients with high SLC3A2 expression have lower OS, DSS, and PFI scores than patients with low SLC3A2 scores, whereas DSS is unaffected (Fig. 6B–J).Figure 6Univariate regression analysis. (
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PMC11476666
A549, lung adenocarcinoma cell line (kindly provided by Dr. L. Iamele), was cultured in DMEM supplemented with 10% fetal bovine serum (FBS), 200 mM L-glutamine, 100 IU/mL penicillin, and 100 µg/mL streptomycin, all obtained from Thermo Fisher Scientific (Waltham, MA, USA), in a 37 °C humidified incubator under atmospheric conditions with supplementation of 5% CO2.
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PMC10831439
In addition, iNKT cells are crucial for efficient immune responses against malignancies and contribute to reduced relapse rates after transplantation.
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PMC11701801
E and F, Expression of CASP6 mRNA (relative to that in siNT-transfected cells) in SMARCA4/SMARCA2-deficient DMS114 (E) and SS18–SSX fusion Fuji (F) cell lines transfected with the indicated siRNAs.
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PMC11394386
To confirm the direct involvement of HIF1α in the transcriptional regulation of HOTAIR, we performed a chromatin immunoprecipitation (ChIP) assay targeting HIF1α, which showed HIF1α binding to the HOTAIR promoter region (Figure 4G).
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PMC11672142
MRC-5 human lung cells were obtained from ATCC (CCL-171) authenticated with a certificate of analysis.
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PMC9429973
Most patients in remission after treatment with FCR or I+V show recovery of normal B-cells at 12 months of stopping treatment.
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PMC11706776
Decrease EWS::FLI1 expression leads to a decrease in KCNN1 expression of 75%, 40%, and 50% in A-673, RD-ES and SK-ES-1, respectively (Fig. 2C).
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PMC9351137
IP sample immunoprecipitated with BAF53a or Tip60 antibodies (+anti-BAF53a; +anti-Tip60) were compared to negative controls (−anti-BAF53a or −anti-Tip60).
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PMC11063646
It arrests cell cycle progression at G0/G1 phase by upregulating the cell-cycle inhibitory protein p27, downregulating CDK4, TNF-α production, and enhancing cluster of differentiation (CD) marker expression (Rajagopal et al., 2003).
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PMC11768281
This review summarizes the contributions of T-cells in malaria, derived from studies on human infections and animal models, both experimental and natural.
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PMC11684269
AKI is categorized into prerenal, intrinsic, and postrenal types depending on the etiology, with each category exhibiting different extents of renal tissue hypoxia (89).
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PMC8010066
The two-way ANOVA analysis revealed a significant strain-dependent effect of S. aureus on the virulence of P. aeruginosa (P-value ≤ 0.001) (Fig. 4e, f).Fig.
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PMC11470999
OE represents cells overexpressing VHL. (
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PMC11578343
as the cell transits the microfluidic zones.
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PMC11687167
It consists of 12 exons (black) and 11 introns (blue).
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PMC3734024
FasL is then moved to the cell surface in response to signals received upon encounter with an appropriate target cell.
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PMC9429973
Pomalidomide is a potent immunomodulatory drug agent with anti-angiogenic, anti-proliferative, and immunomodulatory activity against MM.
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PMC11486946
b, c Phosphorylation of EGFR (b), which was normalized by that in cells after EGF stimulation, and EGFR protein expression (c), which was normalized by that in cells before the stimulation.
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PMC10452486
After activation-induced upregulation, the Face-2 molecules undergo homo- and hetero-dimerization (Face-3 and Face-4).
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PMC11609529
Figure 4Knockdown of both YM and MAML2 in YM-positive cells reduced cell proliferation, enhanced apoptosis, and promoted sub-G1 cell populations(A) Validation of YM and MAML2 knockdown by western blotting analysis.
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PMC9429973
The same approach as applied on MM and EMD.
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PMC11640476
The resazurin-based viability assay serves as a bridge between in vitro experimentation and drug development .
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PMC11243198
From the combined results of both searches, 52 GANT61 analogs were purchased from vendors as listed in Supplemental Table S1.
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PMC11743414
Although treated with traditional regimens, 40% of patients did not achieve complete response, and overall survival was low .
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PMC9031474
Discrepancies in the data could therefore be due to the lack of biological replicates.
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PMC11638255
Given the increase in tumor-infiltrating neutrophils with TEPA treatment, we examined ligand-receptor expression occurring between this cluster and other subtypes and found this was largely driven by the Galectin-9 (Lgals9) and TNF (Tnf) axes which are known to support CD8 cytotoxic T and NK cell-mediated cytotoxicity (Supp.
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PMC11406030
To conclude, there are currently no drugs or immunotherapy programs specifically targeting LMTK3 in clinical trials for ovarian cancer or other cancers.
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PMC11264242
Through a gene ontology (GO) analysis of FLAG ChIP-seq data, it became clear that SS18-SSX2 likely targets distinct sets of genes that are associated with specific biological processes (Figure 1C).
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PMC11462673
Additionally, LDB1 immunoprecipitation successfully captured ETV6, ETS1, ERG, ELF1,IRF1, as well as MYB (Fig. 5D).
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PMC11519567
F Representative image of EdU assay in synovial sarcoma cell line SW982 with the lentiviral expression of EGFP, wild-type and AR mutant SS18-SSX1.
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PMC9532503
The differential affinities of Fc portions of test and control antibodies [due to their different fucose content] to crosslink to FcγR on effector cells lead to drastically different ADCC activities.
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PMC11449273
However, we observed a faint non-specific band in plakoglobin immunoblots (depicted with the asterisk * in Fig 1) with the specific plakoglobin antibody used in this study.
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PMC3765139
However, since the CD133- subset failed to induce xenografts the role of the tumor niche for this particular subset needs to be evaluated in future studies.
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PMC11469524
Fig 2A).
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PMC11574271
Thus, results from these studies confirm that the interaction of the RON mRNA with a particular miRNA exists intracellularly.
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PMC10079526
All DNA damage inducing therapies are toxic, which is part of their effectiveness, and this therapy will not be an exception.
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PMC11676670
HGSOC cannot be characterized by specific somatic mutations due to high variability among patients.
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PMC10761571
According to the Hoechst staining, treating lung adenocarcinoma cells with Fe3O4@Glu-Gingerol caused morphological nuclear alterations associated with cell apoptosis, including chromatin fragmentation, the presence of apoptotic bodies, and chromatin condensation (Fig. 11a and b).Fig.
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PMC9352806
FUDR (120 μM) was added to sterilize the worms during the treatment period.
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PMC11697065
Bound peptides were eluted with 30–80 % MeCN/0.5 % FA and lyophilized.
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PMC10831439
CFSE-labeled cells were tested in a mixed lymphocyte reaction (MLR) and against modified Raji cells.
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PMC11656657
In contrast, no expansion of Mo-MDSC was observed in G-CSF-treated PBMC alone (Supplementary Fig. 15C), indicating that other factors released by the 143B G-CSF cells contribute significantly to the observed Mo-MDSC expansion.
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PMC10538569
Histogram analysis of the expression of the maturation markers CD86, CD83, CD209 and HLA-DR on the surface of mDCs (continuous lines) as compared with iDCs (dash line). (
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PMC11705901
SH-SY5Y were cultured and cells were co-transfected with pGL3-basic vector construct cloned with the gene regions of interest containing the wild type or mutant and Renilla luciferase pRL-SV40 vector by using Lipofectamine 2000.
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PMC11453018
The EWS-FLI1 related genes in the “Reactome Riggi” were identified through the overexpression of EWS-FLI1 in human mesenchymal stem cells.
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PMC11486946
b Optics of the autofocus device.
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PMC11806818
Zhao et al. proposed a dual-mode cellular sensing strategy based on a DNA walker and MOF@Pt@MOF nanozymes to capture and detect MCF-7 CTCs.
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PMC11248911
All melting points were measured using Electrothermal IA 9000 apparatus and they were uncorrected.
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PMC11682224
H&E staining results; (B) Tumor area statistics.
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PMC11509224
A rearranged spongian diterpene, darwinolide (88, Figure 9), has been isolated from the Antarctic Dendroceratid sponge Dendrilla membranosa .
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PMC9919300
In vitro steatosis model was conducted in accordance with the protocol of Campos-Espinosa and coworkers, with subtle modifications .
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PMC7841189
However, the study by Chan et al. showed that LTBP2 expression was downregulated in esophageal squamous cell carcinoma (ESCC) cell lines and tumor tissues, and restoration of LTBP2 suppresses tumor growth in vivo in nude mice12.
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PMC11731614
We created an EV-reporter mouse line by crossing the commercially available CD63-GFP fl/fl mouse line with K14-Cre to create K14-CD63-GFP (KCG) mice.
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PMC11033180
Primary culture of rat enteric neuron was performed as previously described.
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PMC9429973
The median age at allo-HSCT was 47 years (range, 20 to 59 years).
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PMC9684669
The cell lysates were centrifuged at 16,000 rpm for 30 min.
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PMC11720107
Unfortunately, most research focuses on sulfur compounds derived from garlic.
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PMC11678368
HT-29 cells were maintained in RPMI + opti-MEM (1:1) medium (RPMI from IITD PAN, Wrocław, Poland; opti-MEM from GIBCO, Thermo Fisher Scientific, USA), supplemented with 5% FBS (HyClone) and 2 mM glutamine (Sigma-Aldrich, Darmstadt, Germany).
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PMC7090062
To test our hypothesis, we co-transfected GIST-882 and GIST-T1 cells with the miR-30a inhibitor and beclin-1 siRNA.
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PMC11632064
The Seahorse XFe96 sensor cartridges (Agilent Technologies Inc.) were calibrated according to the manufacturer’s protocol.
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PMC9429973
The majority of costs were for iron chelation therapy (68%, $3.7 million) and blood transfusions (30%, $1.6 million).
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PMC11790599
The uptake of lucifer yellow was quantified by flow cytometry (Overmeyer et al., 2008).
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PMC11075223
There are nearly polypinnate compound leaves, rhombic in shape, serrated in edge and stalked in short.
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PMC10823017
An Annexin-V assay was performed in both the Caki-1 and 786-0 cell lines to determine whether the drug treatment induces apoptosis in the cultured cells.
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PMC11026382
Color coding follows from (c), the black dashed line indicates the distance distribution of all residues in the protein.
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PMC11578343
To determine statistical significance, a non‐parametric Wilcoxon signed‐rank test or Kruskal–Wallis rank test was performed in R. All tests used a significance level of α = 0.05.
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PMC5261804
The eigenvector computation is pruned to degenerate results when the smallest eigenvalue is close to 0.
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PMC11721587
Left, Flag-Fu was purified from S100 after in vitro maturation experiments and then incubated with either free Ci or Sufu-Ci, followed by immunoblot analysis with the indicated antibodies.
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PMC11628904
Furthermore, we show here that isotonic medium treatment significantly reduces immune cell recruitment to burned tissue in sterile conditions, supporting a potential role for keratinocyte migration in mediating inflammatory signalling after tissue injury.
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PMC7136814
ERMS has a more complex and heterogeneous genetic profile but has an overall better outcome, as high as 90% 5-year survival for the low-risk group .
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PMC9429973
The median PFS and OS were not reached.
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PMC11588008
Extracellular regulated protein kinases (ERKs), including ERK1 and ERK2, are crucial for transmitting signals from surface receptors to the nucleus.
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PMC11237030
Therefore, we tested the effect of cAMP on our CHO-K1/AQP5 cells (clone 16).
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PMC11116779
The test was performed using six independent experiments for each condition.
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PMC11576293
1.
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PMC11098378
Recombinant Flag-SHOC2 or PSMC1-His proteins were incubated with GST-IpaH4, E1, E2, ubiquitin, and ATP as described above for our autoubiquitination assays.
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PMC3122064
As expected, the combination with Nutlin-3 has no potentiating effect on cisplatin-induced cytotoxicity in the mutant p53 cell line NCCIT (Supplementary Figure 4b).
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PMC11711345
The compounds were tested in vitro following a primary screening similar to the NCI-60 standard protocol for HCCL anticancer drug screening .
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