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PMC11727145
The HaCaT source is a well-established, immortalized human keratinocyte cell line derived from normal human skin that spontaneously transformed .
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PMC11637820
Previous studies speculated that CAP-mediated bacterial inactivation is dependent on peptidoglycan thickness, differences in feed gas, and discharge configurations of the CAP, indicating that gram-positive bacteria are more resistant than gram-negative bacteria [27-29].
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PMC10957991
GAPDH was used as a loading control. (
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PMC11577421
We employed native bovine serum albumin (BSA), cationic albumin (BSA+), and anionic albumin (BSA−) for coating fluorescent silica NPs of 50 nm in size.
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PMC11747949
Survival of patients with localized disease is about 60%, and only 20% in patients with metastatic and recurrent tumors (Meltzer and Helman 2021).
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PMC11127909
Next, we performed IHC staining with EFHD1, Ki67 and C-C3 antibodies and found that CATR inhibited the proliferation of 143B-EFHD1 cells, while BKA promoted the proliferation of 143BR-shEFHD1 cells.
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PMC11754432
The data here builds on this mechanism, showing reduction in lipid peroxidation provokes reduced ERK phosphorylation via NRF2 and EGFR signalling (Fig. 8).
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PMC9436459
Sorocenols C and D (47 and 48) were obtained from the root bark of Sorocea bonplandii, which were racemic mixtures due to their zero optical rations .
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PMC11674146
In short, we found that CLP36 expression correlates with the prognosis of lymphoma patients and that silencing CLP36 expression leads to reduced proliferative capacity and increased apoptosis of lymphoma cells, which correlates with inhibition of the CLP36/PI3K/AKT/CREB axis.
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PMC11707577
The presence of EGFR drives the vector in the tentacles, showed by in silico analysis to express putative Hydra EGFR homologues.
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PMC10728200
B GIST-T1 and 882 cells were treated with IM (50 nM for T1 and 200 nM for 882) for 24 h in the absence or presence of Ferrostatin-1 (1 μM), and the cell viability was assayed.
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PMC11741577
In terms of HMC-1 CM-treated groups, the results on day 3 indicated that the wound surface in the CM-treated group was nearly obscured by a translucent membrane compared with that in the control group.
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PMC8481254
Moreover, IF experiments showed that knockout of CDK19 reduced YAP nuclear localization (Fig. 6D).Fig.
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PMC9031474
Compound 18 was 10- to 20-fold more active than polygodial (2), showing an increase in cytotoxicity by the conjugated π-substituent.
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PMC11805741
The spectral characteristics of carotenoids can be estimated through photodiode array detector (PDA).
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PMC11769516
Studies have mainly focused on the use of isolated phytochemicals classified as polyphenols.
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PMC10958426
The results also indicated that cancer cells expressed much more genes, and stronger cell-cell communication with other cell types than their normal counterparts.
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PMC10778532
We demonstrated that VHL-C162F mutation increases cell motility.
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PMC11569208
By conducting a high-throughput screen of a library of silencing RNA (siRNA) targeting human PPs, we uncovered several PPs that robustly enhanced infectivity and oncolysis of the oncolytic vesicular stomatitis virus (VSV∆51).
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PMC11577421
We first titrated BSA into a solution of fluorescein-labeled heparin and monitored the corresponding changes in anisotropy.
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PMC11502443
However, there are currently a suite of new GIP receptor targeted therapeutics in development (Ahlqvist et al., 2013; Saxena et al., 2010; Lynn et al., 2003; Almind et al., 1998).
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PMC11546117
Stock solutions (100 mM) of fexofenadine hydrochloride for P-gp (ABCB1) function, CDFDA for MRP2/3 (ABCC2/3) function, sulfasalazine for BCRP (ABCG2) function, and methotrexate hydrate for OAT (SLC22A6/7/8/9) function were prepared in DMSO.
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PMC11697065
To reduce this variation, alternative approaches could be leveraged in future studies.
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PMC11434983
Four replicates were performed for each concentration.
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PMC11628904
Using cultured human skin, we found that topical treatment with isotonic solution (saline) similarly prevented the spread of epithelial damage over time.
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PMC5874706
This may be due to the THN7 molecule′s better affinity for these fluorinated derivatives at the molecular scale, implying either more molecules inside the particles, or THN7 coating on the surface.
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PMC10936243
Theme 2: Inadequate practice management skillsMultiple roles.
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PMC10079526
Equal variability of groups is assumed, otherwise Geisser-Greenhouse correction is implemented.
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PMC9429973
Results: Trial in progress.
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PMC10940855
Flasks or 24-well plates were precoated with 10% gelatin (Sigma Aldrich, G1393–100ML) for 30 minutes, then washed with PBS.
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PMC11604768
c Dose response curve from HEK293 cells transfected with an AR promoter dual luciferase reporter and treated with dexamethasone (AR, minCMV, rotation 4, spacer set 1).
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PMC10033453
Results were analyzed by unpaired t test.
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PMC10443665
CCRF-CEM/R, treated with APS (500 μg/mL), tragacanthin (2 and 10 μg/mL), or bassorin (20 and 100 μg/mL).
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PMC7887261
It is of note that in our dataset, none of the variant allele carriers of the 25 candidates were found to have African-ancestry.
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PMC11486946
Observed schemes of the hit compounds.
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PMC8068766
Conventional therapies have low efficacies due to resistance and off-target effects in HCC.
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PMC11726183
The conformation generation was set to 32 for ligands and only least energy conformers were used for docking analyses.
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PMC11509224
The compound gracilin J presented neuroprotection effects at the mitochondrial function level.
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PMC11461874
On the other hand, TRAF7 promotes K48-linked ubiquitination of p53, followed by proteosome-dependent degradation of ubiquitinated p53.
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PMC11684297
The samples were washed with methanol to remove any impurities and dried in a dirt-free atmosphere before proceeding to make the KBr pellet.
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PMC10747160
Although COVID-19 transmission has been reduced by the advent of vaccinations and a variety of rapid monitoring techniques, the SARS-CoV-2 virus itself has shown a remarkable ability to mutate and persist.
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PMC11366496
Combining RNF122 inhibitors with existing treatments such as radiation therapy, chemotherapy, or other targeted therapies such as EGFR inhibitors may enhance therapeutic efficacy, especially when there is synergistic effect on specific signaling pathways.
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PMC11705630
Deoxynucleosides (dNs) including deoxyadenosine (dA), deoxythymidine (dT), deoxycytidine (dC), and deoxyguanosine (dG) were purchased from Sigma-Aldrich, MA, USA.
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PMC11528603
Phenolic compounds can be found in SA-EA and SA-H2O while SA-EtOH and SA-PE contain saponins.
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PMC9429973
Summary/Conclusion: This report sheds light into outcomes of pts with HM infected with Omicron variant.
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PMC11718809
The number of exons within circRNAs ranged from 1 to 20, with the majority clustered between 1 and 5 exons (Figure 1D).
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PMC11468365
This paper has proposed the MultiComb model, an MTDL model designed to predict both the synergy and sensitivity scores of drug combinations simultaneously.
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PMC10723784
It should be noted that the application of CBD in clinical settings did not induce significant side effects or detrimental effects on physiological parameters (heart rate, blood pressure, and body temperature) and psychological functions.
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PMC9841531
Mean ± SEM values of at least three independent experiments are shown.
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PMC8540692
Contrarily, U-138 MG cell line was the most susceptible to the treatment with CCM (Figure 4C).
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PMC7305184
Cells from the third generation (CTRL, CTRL + EP and EP group) were harvested and resuspended in complete HAM F-12 growth medium to the density of 2 × 10 cells/ml.
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PMC9429973
Sixteen (38%) pts discontinued acala, most commonly due to disease progression (26%).
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PMC9429973
Summary/Conclusion: In this work we report MCT1 inhibition as a strategy to treat invasive and metastatic cancers.
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PMC10933092
N1-methyladenosine (mA) localizes at all chromosomes and at transcription start sites, CDSs, and 5′-UTRs of transcripts. (
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PMC11499955
With the help of a click-and-release bioorthogonal reaction, the method allows (i) localisation of the antigen receptors set through fluorescent labelling and (ii) specific identification and quantification of the different antigen receptors through the release of MS-tags.
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PMC11659921
We visualized the relationship between risk score, survival time, survival status, and the expression levels of ANGPTL4, COL8A2, CX3CR1, ERBB2, JUP, PI3, and TNFSF11 in the TCGA‐BLCA dataset using a heat map (Figure 5C).
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PMC9813422
The comparison analyses between EBV-associated gastric cancer cell lines and normal gastric epithelial cell lines revealed that EBV episome attachment induces heterochromatin to euchromatin transition.
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PMC9727330
In conclusion, the expression of ASNS in cancer cells is increased to fill the cell need for Asp, which cooperates in many biological processes related to cell proliferation and cancer development.
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PMC9429973
Only 5 countries reported Bosutinib available: Argentina, Colombia, Chile, Nicaragua and Venezuela.
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PMC10490530
Therefore, during this study, the native Luz gene was suitably adapted for applications of human cells by performing rational mutagenesis of 187 bases.
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PMC11786704
After washing with PBS, cells were treated with fresh DMEM or DMEM-containing PC capsules and incubated at 37 °C.
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PMC9351137
RNAi knockdown experiments were performed by transfecting S2 cells with specific siRNAs against each protein (see “Methods”).
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PMC11794588
Bhuvaneshwaran et al.reported that β-amyrin suppresses liver cancer cell proliferation and induces apoptosis through ROS generation.
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PMC11307990
The cells were incubated with a mixing solution including 6HB-DONs, SUN, and DNA nanotape-SUN with 10% FBS in 1640 medium at 37 °C, separately.
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PMC9429973
D. E. Vybornykh, T. Moiseeva, E. Gemdzhian, T. Gaponova, D. Novikova NATIONAL RESEARCH CENTER FOR HEMATOLOGY, Moscow, Russia Background: The issue of factors influencing adherence to treatment of patients with hematological malignancies (HM) has not been sufficiently studied.
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PMC9844987
In support, genetic variants may affect promoter shape for ubiquitously expressed genes in flies with limited effect on promoter expression (Schor et al., 2017).
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PMC11267036
Previous research showed that the STAT1/RIG-I axis has significant implications for tumor development and pathogenesis .
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PMC10976516
Surgery is the preferred and most effective treatment for stage I and II diseases.
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PMC11634027
We identified and deleted six different conserved motifs within the Ds-ICD.
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PMC11746948
GPX4 protein levels detected by immunoblots (m) and relative mRNA levels by RT-qPCR (n) in A375 cells infected with various GPX4 constructs.
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PMC11787381
The animals were kept for a period of acclimation of a minimum of 5 days.
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PMC11708541
When 4-AP was present, there was a significant (P < 0.01) drop in MAO activity, and this drop was further pronounced when deuterated 4-AP was present.
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PMC11547972
After 24 or 48 h, cells were fixed and detected using anti-BrdU mAb, according to the manufacturer’s instructions.
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PMC11721096
S0, S5, and S5-blank were analyzed using HPLC under the following conditions: UV detection wavelength of 220 nm, column temperature of 35 °C, injection volume of 20 μL, mobile phase consisting of solvent A (0.1% formic acid/water) and solvent B (methanol), gradient elution of 30–100% B over 0–35 min, and a flow rate of 0.8 mL/min.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", ...
PMC7452526
A simple, inexpensive, eco-friendly and green method using an aqueous extract of Eucalyptus camaldulensis leaves has reported for synthesis a small size silver nanoparticles 12 nm without any protecting or capping reagents.
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PMC11388384
In the presence of SCUBE1, SHH-Halo still diffused as monomers in four states (Fig. 3F and SI Appendix, Fig. S9).
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PMC10772747
This indicates that the tested compounds in EBE have the potential as therapeutic agents or drugs in the treatment of breast cancer due to the protein-protein interactions between these compounds and the target genes.
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PMC9429973
These analyses are not powered for statistical comparison; small sample sizes in some subgroups result in wide confidence intervals and, although supported by several sensitivity analyses, the observed trends must be interpreted with caution.
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PMC11621493
To study its impact on gene expression in primary keratinocytes, we performed RNA sequencing analysis of KO and WT cells.
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PMC11347429
The respective PLINK file for the combined dataset (SIG_geno_combine) is provided in Data set 19 (see Table 1 for availability of supplementary data and tables).
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PMC10114490
C RNAseq extracted dendrogram/heatmap (sample and gene-wise) of genes present in DETOX gene set (GOBP: detoxification).
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PMC10713411
We think that |PCC| >0.3 and P-value < 0.05 represent the existing correlation between the two groups of data.
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PMC11389534
In 293T cells infected with SenV, increased interaction between 14‐3‐3ε and UFL1 leads to UFMylation and subsequent binding to K63‐ubiquitinated RIG‐Ι, enhancing downstream IFN signalling.
[ { "tags": [ "O", "B-CellLine", "I-CellLine", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "...
PMC11792090
Residual CD3 T cells were then removed using CD3 positive selection magnetic beads, and cell expansion was conducted in a wave bioreactor.
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PMC8041314
As ABCB1, ABCC1, and ABCG2 have their individual “preferences” regarding inhibitors, finding simultaneously interfering agents is quite an obstacle, which distinguishes C@PA from other approaches in the literature.
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PMC11626627
Additionally, immunohistochemical staining revealed that knockdown of ERβ increased E-cadherin expression and decreased N-cadherin expression, while overexpression of TWIST1 partially reversed the effects of sh-ERβ#2 (Supplement Fig. 2).
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PMC11473843
An average of 37 million reads were generated/library.
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PMC11779605
Differential expression gene analysis. (
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PMC9429973
No pts discontinued INO due to toxicity, and no cases of veno-occlusive disease have been observed.
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PMC11697189
Images of the cells at various time points were captured under an inverted microscope for analysis.
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PMC11770746
All chemicals and solvents were procured from authentic commercial sources and used without further purification.
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PMC11470999
After treating B16 and A375 cells with different concentrations of PF for 24 h, Annexin V‐PE/7‐AAD staining was performed, followed by flow cytometry to assess the effect of PF on cell apoptosis. (
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PMC11742431
Through continuous experimentation on this platform, solid and effective targeted degradation proteins can be obtained, providing a specific theoretical basis and support for developing novel degradation drugs.
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PMC3675084
Cells were pretreated with caspase-3 inhibitor (20 µM Z-DEVD-FMK) or negative control inhibitor (20 µM Z-FA-FMK) for 24 hours before incubation with 10 nM BPR0L075 for additional 72 hours.
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PMC9429973
Frequently used salvage therapies include chemoimmunotherapy (CIT), proteasome inhibitor (PI) based regimens and Bruton tyrosine kinase inhibitors (BTKi).
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PMC11626627
p < .05, **p < .01, ***p < .001, ns, not significant compared to the controls.
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PMC8382973
The 2P23 peptide must remain stable at different temperatures during the shelf life of the compound and in the human body during the manufacture of microbicides.
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PMC9250505
Interestingly, inhibition of PARP activity by Olaparib in EOC cells has also been reported to stimulate the RAS/MEK/ERK and PI3K/AKT/mTOR signaling pathways.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Interes...
PMC10926885
We also analysed the effects of PAFAH1B1 and DCTN1 on OS in various cancers, and found that the high expression of these two genes is associated with poor prognosis of various cancers (Figure S4).
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PMC5963610
A recent in vitro study demonstrated that EGFR phosphorylation in A431 cell lines is less inhibited in the presence of NGcGM3 than in the presence of NAcGM3 .
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