PMCID
string
Sentences
string
ner
list
PMC11291490
n = 3).
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PMC9429973
Six cases received CD7-CAR-T therapy, and all achieved disease remission after CAR-T cell infusion, of which 1 died from secondary severe lung infection and 2 died from relapse.
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PMC11733919
Samples were revealed using SuperSignal West Femto kit following the manufacturer’s protocol and the results were visualized using a Chemidoc imaging system (Bio-Rad, Madrid, Spain).
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PMC11627060
BSA NPs peak slightly shifts due to alteration in the protein backbone which substantiates that BSA develops into a nano-sized molecule.
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PMC11763764
In the first-in human phase 1/2 trial, FGF401 treatment alone was safe and showed preliminary clinical efficacy in patients with hepatocellular carcinoma .
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PMC9429973
Summary/Conclusion: The combination of BCL2 and MCL1 inhibition is a potential effective treatment in ATO/RA resistant APL patients and could be clinically explored in this category of patients with very poor prognosis.
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PMC11628904
Quantification of neutrophil and macrophage recruitment at each time point was performed by measuring the total thresholded area of fluorescently labelled cells, respectively.
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PMC11279397
The KEGG pathway enrichment analysis identified the top 20 enriched terms in Perilla frutescens using the DAVID database, revealing associations with pathways involved in cancer and viral infection.
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PMC11767725
A study by Bousbaine et al. (2024) highlights the skin’s ability to generate a potent, durable antibody response against Staphylococcus epidermidis, a common commensal.
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PMC5415764
The cells were collected by centrifugation and stained with 5 μL of PI solution (Life Technologies, USA) for 10 min.
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PMC11412752
Our results showed that the optical density (O.D.) values of the STING knockout group were significantly lower compared to the WT group at several time points after incubation with the CCK-8 reagent (Figure 2).
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PMC11448304
As shown in Fig. 4b and c, 626 of 2-hydroxyisobutyrylated proteins were downregulated in RA549, while 732 of them were upregulated compared to A549.
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PMC11755467
--R2 <untreated_R2_read_file>: Path to R2 read file for untreated samples.
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PMC11718817
However, nigericin treatment of macrophages resulted in a slight decrease in the arrest coefficient (dark gray), also found for early infiltrating Il18r cells (dark orange).
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PMC11371747
We compared dl-LV with l-LV and investigated whether d-LV would interfere with the activity of l-LV.
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PMC8540692
Samples were run in duplicate in each experiment, and each experiment was repeated twice.
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PMC11194678
Scale bar: 5 μm. (
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PMC11637284
Due to the C-terminal truncation it was not affected by the RNAi.
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PMC9351137
After RNAi knockdown, the total amount of each CRS decreased, as well as the fluorescence intensity of nuclei, spindles, and midbodies (Fig. 3, Additional file 2: Fig. S2).Fig.
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PMC9429973
Secondary outcomes included SRoT duration and platelet count changes from baseline.
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PMC11731161
F NCI-H929 cells were transfected with indicated tet-on-shRNA, cells were treated with 10 µM MG132 for 6 h prior to collection, then XPO1 were immunoprecipitated with anti-XPO1 antibody, ubiquitination levels of endogenous XPO1 protein were analyzed by immunoblotting using the ubiquitin antibody.
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PMC9509578
Their results clearly showed inhibition of tumor development and metastases .
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PMC10831439
CD19-CAR-iNKT cells maintain their ability to prevent T-cell activation and proliferation. (
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PMC9429973
NX-2127 has shown preclinical activity similar to IMiDs by catalyzing the ubiquitination of Ikaros (IKZF1) and Aiolos (IKZF3), ultimately leading to increased T-cell activation.
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PMC11487720
Furthermore, BCD, unlike apoptosis, is an irreversible cell death event .
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PMC11412721
Cells were grown according to a 3T3 protocol.
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PMC11129910
One patient developed invasive ductal carcinoma after two years of initial diagnosis.
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PMC9878278
Next, to assess whether BMP6 affects ZIKV replication, SC were treated with BMP6 at the concentration of 60 and 100 ng/mL based on other studies (Wang et al., 2017) for 24 h before infection and then replenished with fresh BMP6 every 24 h. We found that only 100 ng/mL BMP6 treatment resulted in an increase in viral titers at 48 hpi and a decrease by 50% at 120 hpi as compared to untreated SC (Figure 6C).
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PMC9250505
A rational and efficacious combination strategy involving combined inhibition of pBADS99 and PARP for the treatment of recurrent EOC is presented.
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PMC11300085
RhoA/ROCK activation also induces epithelial-to-mesenchymal transition (EMT) that exerts a vital role in tumor cell metastasis, especially for OC cells .
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PMC11634027
Separating functions encoded by the Ds D region. (
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PMC11525293
The blots were incubated with the following antibodies (Abs): anti-p63 4A4 (Santa Cruz Biotechnology sc-8431), anti-ACE2 (Abcam ab15348), anti-CRBN (Cell Signaling Technology D8H3S), and anti-actin (Santa Cruz Biotechnology sc-8432).
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PMC7723249
Others have proposed that microvascular leakage, directly induced by the virus and/or as a result of an overreacting host immune response, cause the hemorrhages seen in CCHF .
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PMC7917457
In order to assess if coating VALO-D102 with 6K-NY-ESO-1(91−110) or 9K-MAGE-A3(161−180) peptides had an effect on virus aggregation, we compared both mono-coated viruses and a mixture of the mono-coated viruses (PeptiCRAd-1) to naked VALO-D102 using NTA.
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PMC10297204
The inhibitory effect of ethanol on the motility of HS-SY-II was assessed with a wound healing assay.
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PMC7171147
h Western blot of pAMPK (T172) in OVCAR3 cells cultured in either PBS or COL11A1-coated plates for 48 h and treated with MK2206 (20 µM) for 48 h. GAPDH was used as a loading control.
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PMC7812570
The entry of HIV-1 into target cells is mediated by viral envelope (Env) glycoproteins: the surface subunit gp120 is responsible for binding with the primary cell receptor CD4 and a coreceptor (CCR5 or CXCR4), which triggers substantial conformational changes in the viral Env complex, while the transmembrane subunit gp41 is involved in virus-cell fusion by inserting its fusion peptide into the cell membrane and then folding a six-helix bundle (6-HB) structure between its N- and C-terminal heptad repeat regions.
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PMC11791264
The lysates were immunoblotted with the antibodies indicated on the right.
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PMC9429973
NK cell activation was induced more potently than by an effector-enhanced IgG1 and was accompanied by more pronounced degranulation and IFNγ production.
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PMC9429973
For two of these variants an impaired function had been already described in the literature.
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PMC11730320
The family was subsequently offered research based WGS with parental consent (REB study 1000005598).
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PMC10178190
Based on these results, we further explored ERK1/2 signaling’s role in soluble factor-mediated chemoresistance.
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PMC9429973
However, few prospective studies evaluated the value of combination of pomalidomide with bortezomib and dexamethasone (PVD) in these special patients.
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PMC11720808
OS cells were pretreated with 100 μM SCU or DMSO, then divided into six equal portions in PCR tubes, and heated at 45, 55, 60, 65, 70 and 75°C for 4 min.
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PMC11266100
Cultivation of cells was carried out at 37°C with a 5% CO2 atmosphere.
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PMC11661040
H-I) Colony formation experiments revealed that the activation of Wnt pathways weakened the suppressive effect of CGREF1 on cell colony formation.
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PMC7757104
GraphPad Prism version 5.02 (GraphPad Software, Inc.) was used to plot the graphs.
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PMC8973085
IR (KBr, v, cm): 3396–3209 (NH2), 3139 (NH), 2228 (CN), 1653 (C=O); H NMR (DMSO-d6): δ 2.33 (s, 3H, CH3), 2.53 (s, 3H, CH3), 7.15–7.36 (m, 10H, 8CH-Ar + NH2), 10.54 (bs, 1H, NH); C NMR (DMSO-d6): δ: 20.30, 21.40, 114.10, 115.50, 119.40, 123.60, 126.30, 126.60, 127.50, 130.60, 131.40, 132.30, 135.30, 136.50, 157.40, 163.50, 164.80, 165.60, 167.10; MS m/z (%): 416 [M] (8).
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PMC9872547
Approximately 10 cells were seeded in each well of 96 well-plate.
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PMC11788920
In agreement with these expectations, we observed the c-Myc mRNA levels dropped to 0.28 ± 0.08 of control when both RNP2 and RNP3 were introduced to the cells (Fig. 1B), which is beyond the reported suppression achieved with GQ-stabilizing small molecules (∼0.50-fold in the presence of 100 μM TMPyP4) .
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PMC10222971
Therefore, there is still a pressing need to develop less harmful and cost-effective therapeutic alternatives; the discovery of novel anticancer drugs based in natural products is a key research field that has attracted a great interest in recent years.
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PMC6742971
Also in anti-tumoral T cell responses, generating and maintaining a relatively large number of clonotypes may be more powerful for both safeguarding the flexibility of the repertoire and ensuring an effective immune response.
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PMC11745600
Male mice that received porcine islet grafts promptly exhibited lowered blood glucose levels, maintaining blood glucose levels below the normal glucose range for at least 52 wk posttransplantation.
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PMC9429973
Results: Four patients with newly diagnosed AA were identified.
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PMC7351993
The cross-sectional dimensions of the central microchannel are 200 × 200 µm.
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PMC9596868
No. 0503) under standard cell culture conditions.
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PMC3813807
All transfections were carried out using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA), according to the manufacturer’s instructions.
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PMC11502443
The data was then fitted to the Operational model.
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PMC9428827
Levin et al. also described an IL-2 mutein with agonistic properties (15); H-9 has an increased affinity for the β chain of IL-2R, and although it conserves the capacity to induce Treg proliferation, also induced an outstanding effect on CD8 cells and induces higher antitumor activity than wtIL-2.
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PMC11367146
The active site of the receptor protein with a higher pocket score was selected using the online server Prankweb (https://prankweb.cz).
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PMC10421378
Adhesion can be analyzed using various in vitro and in vivo methods.
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PMC11781181
Subsequently, we tested whether exosomal miR-375-3p was involved in keratinocyte ferroptosis in patients with SJS/TEN.
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PMC9429973
Its initial findings indicate potential prognostic significance of GAS5 expression and the need for further investigation of these two non-coding RNAs, their potential roles in leukemogenesis, and the prognosis of AML patients.
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PMC11739504
Nuclei were stained with DAPI (blue). (
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PMC11591038
Neurogenesis—Sleep apnea is known to affect mental performance and may also contribute to neurodegeneration in AD .
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PMC11683240
The 488-labeled PT and α1AT were either directly added to cells for 15 min or preincubated for 15 min before addition to cells.
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PMC5600151
Table 2 presents a summary of the dendrimer-based nanosystems described further in this section.
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PMC11300085
However, it has also been reported that chemerin in vitro promotes or does not affect the growth of ovarian cells, such as OVCAR-3 .
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PMC11462673
Additionally, we performed stem cell marker CD34 analysis on Jurkat and PF-382 cell lines using flow cytometry.
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PMC10571053
Cells were then treated with increasing concentrations of the desired compound and incubated for 72 h. Cell TiterGlo (Promega) was then used to determine luminescence values for each concentration according to the manufacturer’s instructions.
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PMC7039683
While direct connections inferred in this way await confirmation by ChIP-seq or CUT&RUN, cross-regulation among members of a given GRN layer is a common feature in development (Davidson, 2009).
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PMC11765243
Thus, the overall effect of T-cell-specific deletion of p110α can be beneficial or detrimental depending on the specific response and parameter considered.
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PMC11638764
This compartmentalized effect may offer valuable insights into p97/VCP function.
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PMC11348300
Fig. 4D depicts PpIX distribution in blood over time, mirroring the overall tissue distribution (Fig. 4E).
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PMC11665584
The cells obtained from control skin (n = 8 mice), PITX1 skin (n = 8), and buccal mucosa (n = 7 pools) for sequencing and analysis were pre-processed and screened for quality control features prior to bioinformatic integration using the Seurat platform and manual annotation of resultant clusters to generate generic cell type categories (Supplemental Figure 2, A–E, and Supplemental Methods) (17, 18).
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PMC11590005
It focuses on the belief that Blacks and Whites are essentially different .
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PMC9429973
Aims: In this study, we aimed to identify immediate critical effectors of the NUP98-KDM5A fusion protein and to characterize the transcriptional programs through which they regulate the development and maintenance of NUP98-KDM5A-driven AML.
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PMC10988555
It was used at least 7 independent chicken embryos experiments for each condition.
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PMC6600253
Song et al. showed that gemcitabine significantly increased the levels of senescence-associated molecules, including p53, p21, p19ARF, PML, and DCR2, in Miapaca-2 and Panc-1 cells, with the exception of p53 in Panc-1 cells .
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PMC9429973
Determine the prothrombotic potential of PV-exosomes and their effect on endothelium.
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PMC11024707
cDNA was obtained from the isolated mi-RNAs using the cDNA isolation kit (Applied Biological Materials).
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PMC11768281
These cells express a unique γδ T-cell receptor (TCR) that distinguishes them from conventional αβ T-cells.
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PMC8658661
In addition, the liver takes part in the metabolism of nutrients, receiving digestive products in the form of glucose, amino acids, fatty acids, and glycerol.
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PMC6133382
A major goal in HIV vaccine research is to develop immunogens that elicit bNAbs.
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PMC11765678
MDR not only limits effectiveness to one class of drugs but to a broad range of anticancer drugs complicating treatment by causing resistance to unrelated drugs.
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PMC9454852
GraphPad Prism 8 (Graphpad Software Inc., GraphPad Software, Inc., San Diego, CA, USA) was used to perform statistical analysis.
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PMC8633974
This can be attributable to mismatches between target DNA and gRNA protospacer adjacent motif (PAM, seen with TatF) or in seed sequence proximal to PAM (seen with TatE2) (Figure S6).
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PMC11670407
Transfection was conducted using Lipofectamine 3000 Transfection Reagent (Thermo Fisher, UT, USA) in accordance with the manufacturer's instructions.
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PMC11531735
Additionally, we used a neoadjuvant model with the MMTV-erbB2 (Fo5) transgenics and the tumor-free survival rates exhibited a remarkable difference between the control and treated groups when ER121 was administered orally.
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PMC10578720
Enzymatic activities of CS and PDHC can be inhibited in macrophages by HBV-induced hyperacetylation. (
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PMC11591794
In addition, unstained cells were imaged and examined by phase contrast microscopy.
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PMC8414691
From left to the right: DAPI (blue), anti-α-Tubulin (green), cytokinesis regulators (red) and merge.
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PMC11626627
786-O cells were transduced with: (1) pLKO+pWPI, (2) sh-ERβ#2+pWPI, (3) oe-TWIST1+pWPI, (4) sh-ERβ#2+oe-TWIST1, or (5) sh-ERβ#2+sh-TWIST1#1.
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PMC10745221
After culturing the cells in rMETase, methionine was depleted from the culture medium, so most of the cancer cells did not survive.
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PMC11078693
After the glass lid is placed on top of the over-field chamber, there will be no air gap under the lid, and no meniscus to refract trans-illumination light.
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PMC10213179
Data are presented as means ± SD.
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PMC11628309
Alongside the variability in culture media, there are a wide range of fetal bovine serum supplementation, varying from 5 to 20% (Xicoy et al., 2017).
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PMC11666785
16 hours after seeding, cells are transiently transfected with Dendra2-Actin plasmid using Lipofectamine 3000 following the manufacturer’s protocol (Invitrogen, USA).
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PMC11209164
Gene expression was normalized to GAPDH, and fold change was calculated relative to the mock-treated samples for each gene using the Pfaffl method .
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PMC7553912
Quantitative analysis of the protein expression of OPN, ANXA1, ANXA2, TNF-α, and Col1 in the renal fibroblasts.
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