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OTAR3088
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BioData-CellLine_annotated

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PMC11277157
The KD of DJ-1 highlights its potency in regulating genes associated with antiviral responses, RNA metabolism, and mitochondrial functions, apparently through alteration in STAT activity and downstream signaling.
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PMC6163708
SK-N-SH, SH-N-MC, HT-1080, and T98G cells were maintained in EMEM.
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PMC7642379
Interestingly, the original HEK293 cell line was the most distant from all other cell lines.
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PMC8540692
In fact, the impact of NaBu was as strong as the one obtained using the anticancer drug—topotecan, used as a positive control in this assay.
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PMC10253553
Primers for GAPDH forward 5′-CATGAGAAGTATGACAACAGCCT-3′, reverse 5′-AGTCCTTCCACGATACCAAAGT-3′, Rictor forward 5′-GACTGAAACCCGTCAATATGGC-3′, reverse 5′-TGTCATTCCGCCCTCGTACT-3′, NDRG1 forward 5′-AGCTCGTCAGTTCACCATCC-3′, reverse 5′-GAGTACGCGGGGCTACAAAG-3′, beta-actin forward 5′-CTCGCCTTTGCCGATCC-3′, reverse 5′-TCTCCATGTCGTCCCAGTTG-3′ primers were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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PMC9024365
The script separates the channels, applies the average threshold values, creates the mask overlay, and analyzes cluster parameters (number and size) defined by the mask.
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PMC10317042
CCS cells were seeded at a density of 5 × 10 cells/well in 96-well plates or 4 × 10 cells/well in 6-well plates and cultured for 24 hours.
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PMC10253973
The most active complex, 63 (Figure 25), showed significant phototoxicity with a low IC50 value towards human liver cancer cells (Bel7402) and human liver cancer cells (HepG2) compared to 1.
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PMC11770199
Compared to oncolytic viruses, engineered MSCs appear to be a better choice for delivering viral fusogenic proteins to treat cancers because of their lower immunogenicity, which allows for longer persistence in action.
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PMC9220170
Such an effect on the endoplasmic reticulum can significantly alter the expression of proteins that regulate cell viability pathways.
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PMC7802142
To elucidate the biological function of miR-100 delivered by ReN-NV, synthetic anti-miR-100 was transfected 12 h before the co-incubation of DPCs and ReN-NV, and then Western blottings were performed to access β-catenin expression in nucleus.
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PMC11607764
The expression level of PTBP1 gene across diverse cancers types and subtypes were systematically analysed by applying TIMER2; (B) Protein level of PTBP1 was investigated through CPTAC dataset; (C) The expression level of different pathological stages in some cancers were also explored based on TCGA data. *
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PMC6379402
In the main paper, we assess if iCells and their constituent PPI, GI, and COEX networks capture well the functional organization of the cell by clustering genes in these networks and then by measuring the enrichment of those clusters in biological annotations.
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PMC11590870
Thus, their death percentage is considered the normal cell death rate.
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PMC9454852
Malignant pleural mesothelioma is considered to be a rare cancer worldwide, but it is characterized by high incidence in some regions that appear as hotspots .
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PMC11679892
These alterations underscore the need for targeted therapies that can address these genetic aberrations.
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PMC10933092
The results were quantitatively analyzed using High-sig ECL Western blotting Substrate (Amersham ImageQuant 800, Japan).
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PMC11721587
A weak Gal4 driver, C765, was used to drive the UAS transgene expression at low levels to avoid overexpression (43).
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PMC11225860
Microscopy showed a drastic increase in nucleus size between treatment and control, as 1µM alisertib treated nuclei were more than five times larger than DMSO (Fig. 1c and d).
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PMC9429973
Approximately 88.9% of patients, excluding two unrecorded persons, achieved CR beyond 3 months.
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PMC9429973
Summary/Conclusion: The successful integration of histology and immunohistochemistry, flow cytometry, and next-generation sequencing technologies into one standardized workflow depends on close multidisciplinary collaboration of different clinical and laboratory specialists, which has been greatly facilitated by the recent centralization of diagnostics and care for pediatric cancer patients.
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PMC9954564
Since genes encoding interacting proteins are likely to evolve to preserve proper protein interactions, as well as an appropriate stoichiometry among its interacting components , we employed the multifactor dimensionality reduction (MDR) method to evaluate the possible role of epistatic interactions between HLA-G and its receptors in the pathogenesis of vitiligo.
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PMC11225860
U2932 cells were treated synchronized with a double thymidine block over a two-day period.
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PMC11484188
miR-1343-3p inhibitor elevated Beclin1 expression and decreased the relative expression of p62 (Fig. 4A).
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PMC10237474
A similar protocol was also applied in adherent HEK293S GnTI stable pools generated by the PB inducible system to successfully express a bovine rhodopsin which is an integral membrane protein .
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PMC6461034
In summary, the above differential analyses of phosphoproteomes show that the INKA pipeline can pinpoint target activation and inhibition after perturbation in both cell lines and clinical samples.
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PMC10812665
In the second part of the study, we focused particularly on a subgroup of the screen hits: Rot, MPP and Ber.
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PMC9429973
A total of 198 patients had recorded baseline and post-treatment Hb values.
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PMC11190238
For instance, in the Drosophila embryo, Snail represses the transcription of 2 dorsal-ventral patterning genes sog and brk by blocking the formation of promoter-enhancer interactions when Snail is bound to the distal enhancer.
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PMC11805741
Thus, BCL-2 family members and their regulators are desirable targets for the development of anticancer agents.
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PMC10808409
A previous study found out that the incorporation of TPGS with Pluronic P123 resulted in an enhancement in the in-vitro cytotoxicity effect.
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PMC11594588
Platelets, as one of the essential morphological components of blood, play a particularly important role in the development of acute coronary syndromes (ACSs) and in thromboembolic complications during and after percutaneous coronary intervention (PCI) .
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PMC11769516
Studies have shown that compounds with a similar structural structure to ENN are able to easily enter the mammalian body.
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PMC7215832
Briefly, cells were lysed using cell lysis buffer (Cell Signaling, Danvers, MA, USA), containing 4% protease inhibitor cocktail (Roche Diagnostics, Mannheim, Germany) on ice for 30 min and centrifuged for 10 min at 4 °C at 14,000 rpm.
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PMC9429973
In this trial, the experimental intervention follows a treatment strategy: Patients (pts) with AML in complete remission (CR) post-SCT but at high risk for relapse based on pre-SCT risk factors will start with siremadlin priming monotherapy.
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PMC11754432
Two-way ANOVA (C), one-sample t test (A, D); significance denoted by *P < 0.05, **P < 0.01, ***P < 0.001.
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PMC11401350
H Six days post-transduction T cells were surface stained for CD8 and hCD34 followed by staining for CD44 and CD62L.
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PMC11770199
Quantitative reverse transcription PCR (RT-PCR) was conducted to confirm this finding, showing a significant increase in the transcription of interferon-β (IFN-β), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-12A, and IL-23A after cell–cell fusion (Fig. S6C and D).
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PMC10565698
B. Database-dependent prediction of miRNAs that can bind to circUSP10.
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PMC11477621
C NMR (150 MHz, CD3OD) δ 171.0, 137.3, 130.0, 127.9, 125.9, 123.5, 120.3, 109.9, 101.5, 100.9, 45.8, 32.1, 29.5, 28.0, 25.9, 24.8.
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PMC11395280
Although the cells were either CD4+ or CD8+, CD8+ cells outnumbered CD4+ at a ratio of 2.8:1.
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PMC10443665
APS, Astragalus polysaccharides; MTX, methotrexate.
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PMC11574271
Tumorigenic features of these cell lines were described in detail on the provider’s website (www.cellbank.org.cn).
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PMC11257988
are strengthened.
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PMC11507371
The general ability of CAP to exert a more substantial effect on tumourigenic compared to non-malignant cells has already been described for many cell types in the literature.
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PMC11509224
Only one diterpene was reported to be produced by the Strongylophora sponge.
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PMC11013014
The structure–activity relationship (SAR) showed that the position of the nitrogen atom within pyridine has a notable influence on the anti-proliferative activity.
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PMC11438317
Afterwards, the coverslips were incubated with the appropriate primary antibody in PBS with 1% BSA for 1 h, followed by incubation with the corresponding secondary antibody for 1 h, both in PBS with 1% BSA.
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PMC11522251
The data was shown as the mean SD derived from three separate tests.
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PMC11658074
Visualization of these subpopulations was achieved via distributed stochastic neighbourhood embedding (t-SNE) and uniform flow approximation and projection methods .
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PMC11764090
LIP19/OsOBF1 heterodimer formation improves cold tolerance in O. sativa (Table 2).
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PMC6642070
Luciferase reporter assay of wild type (RRE3-WT), mutated (RRE3-MUT) and knockdown of p53 (RRE3-WT/shp53) of ENO3 p53RRE3 in A-204 cells (n = 3). (
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PMC11462673
Furthermore, LDB1 regulates MYB through remote control of enhancer sequences, providing mechanistic insights into its role in T-ALL progression.
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PMC8494202
Gene set enrichment analysis (GSEA) is a commonly used biological information tool that can simultaneously analyze the expression profiles of multiple genes, enabling accurate identification of a target gene's function mechanism.
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PMC9429973
Quality of life (QOL) was assessed using the Functional Assessment of Chronic Illness Therapy (FACIT)-Fatigue Scale.
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PMC11767793
Essential parts of cell viability tests are positive and negative controls that serve as measurement references .
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PMC7802142
Green represents α-SMA, Blue indicates nuclei.
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PMC8526701
The underlying staining area scores were as follows: none (0), 1–25% (1), 26–50% (2), 51–75% (3), and 76–100% (4).
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PMC7685376
Then, 120 µL of 55 mM iodoacetamide was added to the sample and incubated for 20 min protected from light at room temperature.
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PMC11683240
Pertussis-induced lung pathology involves excessive inflammation and tissue damage, making α1AT an attractive therapeutic agent due to its antiinflammatory and tissue-protective properties in addition to its anti-PT effects.
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PMC7812570
The amounts of the P24 antigen in cell culture supernatants were measured by ELISA.
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PMC11596967
CHO-K1 cells were plated in six-well plates at a density of 3 × 10 cells/mL and treated with 30 µM of Ddes-P3, which was dissolved in dimethyl sulfoxide (DMSO).
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PMC11609529
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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PMC8316344
FPN1 is the major factor to export iron from cytoplasm into the blood flow .
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PMC11766350
This wash step was repeated two times.
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PMC11727306
It has been proposed that the excessive production of cytokines causes the immune system to mistakenly attack self-antigens and damage organs .
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PMC10878518
The ATM score is shown in each photograph.
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PMC8348645
From Figure 2, it can be said that the % inhibition of DPPH varies with different plant extracts and the concentrations used.
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PMC7185206
Treatment of vesicles with TAPI-2 significantly increased the number of ATZ-positive EVs remaining after 24 h, suggesting that ADAM10 and ADAM17 continue to cleave PD-L1 after tumor EV release.
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PMC10938377
Nonetheless, beyond a preference for solvent-accessible N7 sites of guanine, these platinum drugs bind otherwise indiscriminately to the DNA.
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PMC11641630
Specifically, the risk of progression from NMIBC to MIBC in high-risk NMIBC patients is up to 50% .
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PMC9429973
Concerning disease status, 39.5% were in complete or partial response, 42.1% had progressive or stable disease and 18.4% were on watch and wait.
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PMC10820104
Optical rotations were determined using a Jasco P-2000 polarimeter.
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PMC4695073
Survival analysis revealed that there was significant difference for OS and PFS between IIIA and IIIB subgroups (Supplemental Figure S4B, S4C).
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PMC11657744
Fig. 2CEP83, CEP164, and SCLT1 are essential for ciliary assembly in NIH3T3 cells. (
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PMC11739696
Different letters indicate significant difference at p < 0.001.
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PMC8345486
Moreover, compounds 23 and 22 induced a decrease in plasma membrane potential (PMP, p < 0.01, Figure 5C, left), which together with the incorporation of PI into the cells, indicated a dysfunction or even damage to the plasma membrane.
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PMC11612626
Compound A effect on SIINFEKL–MHC-I presentation and the murine immunopeptidome.
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PMC9429973
MR4 was noted in 11/68 (16%) and 9/36 pts (25%) at 6/12 mo.
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PMC9739791
NCs 10 were also more effective as a drug delivery vehicle as its level of intracellular platinum in A2780cisR cells after 2 h of incubation surpassed one of the Pt(IV) prodrug 9 by 20-fold.
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PMC11612794
Then, we found that the M phase proportion in CENP-C/CENP-T/Dsn1 cells was significantly increased compared to that in CENP-C/CENP-T/Dsn1, CENP-C/CENP-T/Dsn1, or CENP-C/CENP-T/Dsn1 cells (Figure 6D).
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PMC9454178
Despite the clinical success of tyrosine kinase inhibitors (TKIs), more than half of GIST patients develop resistance due to a second mutation.
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PMC11591794
Although P53 gene expression was determined at a detectable level in the group in which only DOX was administered for 48 h, it was not statistically significant in the control group.
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PMC9429973
Enzyme-linked immunosorbent assay kits were used for the quantification of IL-35.
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PMC10165258
The fluorescent images were acquired using a classical epi-fluorescence microscope.
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PMC11310713
This aligns with the fact that the pharmaceutical HRT, which is a combination of E2 and P4, increases the probability of breast cancer .
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PMC10204952
Results were presented as mean ± SD.
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PMC11449273
Compared with H1299-p53-WT cells, H1299-p53-175 and -273 cells exhibited increased colony formation by ~55% and 62%, respectively (Fig 3A).
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PMC11323699
It is worth noting, however, that this previous study quantified TSP-1 in serum, not plasma, and reported median TSP-1 levels significantly lower (118 ng/ml) than those observed in our study.
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PMC11519583
1, BD Biosciences).
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PMC11768927
Their findings demonstrated that rVSV-ΔM51 exhibited significant cytotoxicity against PDA cells in vitro, effectively reducing cell viability and inducing apoptosis.
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PMC9429973
A median patient age was 34 years (range, 20-55), 22 patients were female, 21 - male.
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PMC6182045
Clade C gp120 proteins expressed in 293 HEK cells were compared by FIA for binding to a panel of five prototypic bNAbs.
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PMC11545868
Apoptosis and antitumor effects of prunetin (in vivo).
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PMC6835428
Jones et al. prepared triblock copolymers consisting of hyperbranched polyethylenimine-graft-polycaprolactone-block-poly(-ethylene glycol) (hyPEI-g-PCL-b-PEG) with and without a FA ligand for FR targeting (Table 6).
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PMC8357004
Results were obtained from three independent experiments and were expressed as mean ± standard error of the mean.
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PMC9046263
While our work has formed the basis for ERVK3-1 to be considered a potential prognostic marker for GBM survival, development of a curated database with tumor samples sequenced for HERV and ERVK3-1 expression would be highly beneficial for future clinical application of our findings.
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PMC9429973
Among patients who developed immunity, 38% were naïve while 30% were off therapy.
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PMC11612315
The cells were held at −60 mV. Exendin 9–39 (100 nM) was delivered via either the external (intracellular) or internal (extracellular) solution in the presence of 10 nM CAP externally.
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PMC11661024
Then, the plates were incubated at 37 °C for 4 h in a humidified atmosphere with 5% CO2 .
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