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PMC6742971
All datasets generated for this study are included in the manuscript/supplementary files.
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PMC11721295
To recapitulate KRAS-dependent paracrine signaling mechanisms stemming from human fibroblasts, we incubated CCL210 human adult lung fibroblasts in TCM from A427 cells treated with MRTX or equimolar concentrations of DMSO (Supplemental Figure 6B).
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PMC9878562
Our data showing the involvement of the LTB4/BLT1R axis in VAL cell migration, could open a way for the development of novel therapeutic approaches in the treatment of DLBCL, potentially in combination with inhibitors of ACKR3 (69–72).
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PMC11297139
We used antibodies recommended by the manufacturer for the species and application.
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PMC6133382
The binding of a panel of bN-mAbs to purified A244-rgp120 produced in MGAT1 CHO cells, CHO-S cells, and HEK 293 GnTI cells was measured in an FIA.
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PMC11674891
We then conducted differential gene expression analysis using DESeq2 based on these count values and performed gene set enrichment analysis (GSEA) .
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PMC10932641
lncRNA GPRC5D-AS1 and internal reference gene GAPDH primers were synthesized by GeneCreate Company (Wuhan, China), as shown in Table 1.
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PMC11629461
These calcium oscillations induced by UVA irradiation or photodynamic action were all inhibited by the pan‐NOX inhibitor DPI and blocked with O2 quencher Trolox‐C. Similar effects of UVA irradiation and SALPC photodynamic action were observed in the human thyroid follicular cell line SW579 and in DUOX2‐CHO‐K1 cells.
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PMC9429973
Results: The baseline characteristics of the groups are shown in Table 1.
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PMC11471176
A similar protocol was followed for the human ACE-2 ELISA kit method.
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PMC10530622
Summing up, oxidative stress underlies oxalate AOPs, which include players as small molecules (e.g., GSH), enzymes (e.g., NADPH oxidases, SOD1, CAT) and transcription factors (e.g., Nrf2 and BMAL-1).
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PMC11725127
P < 0.05, P < 0.01, P < 0.001, P < 0.0001 versus control group.
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PMC5810978
HuNoV RNA levels in HT29.Cl-E1 were similar (2.2-fold) between these two media, and did not increase substantially.
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PMC11653533
Starr et al., 2003).
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PMC10940855
IPA for diseases and functions demonstrated that genes involved in regulation of cancer, organismal injury, cell cycle, and cellular movement were most significantly enriched in the multiple myeloma–supportive HAECs (Fig. 1G).
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PMC11583010
Inlay: MFI signal recovered from MDA-MB-231 cells stained with anti-hNectin-1 (R1.302), anti-hNectin-2 (R2.477), or anti-hNectin-3 (N3.12) mAbs.
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PMC11730320
This is particularly important in cases with profound T cell immunodeficiency such as SCID and combined immunodeficies (CID).
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PMC7802142
Consistent with the results of 293-NV, the treatments of these two nervous tissue cell-derived NVs did not produce significant higher DPC proliferation than that of PBS (Additional file 4: Fig. S4).Fig.
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PMC9621239
The siRNA (10 pmol) of MYC or the control was transfected into Huh 7 cells (left of the panel) and Bel-7404 cells (right of the panel) in a 6-well dish respectively.
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PMC11012012
Furthermore, NK cells expanded via the engagement of the SLAMF2/CD48-SLAMF4/CD244 axis showed stronger anti-tumor capability and infiltration ability into the tumor microenvironment, leading to an improvement in therapeutic efficiency .
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PMC5035325
Color figure online) Co-expression of EGFR and NeuGcGM3 ganglioside in murine models Staining intensity: 0, no staining; 1, weak; 2, moderate and 3, strong staining Positive cells: 0, no staining; 1, 6-25 %; 2, 26-50 % and 3, more than 50 % Co-expression of EGFR with NeuGcGM3 molecules on lung metastasis.
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PMC11680578
We have measured the number of micro-vessels of the same region at 0 h and after 24 h of exposure to explain the effect of treatment on angiogenesis.
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PMC11529598
p < 0.05.
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PMC9776316
Indeed, the DNA damage response and repair pathways have been interrogated intensively to dissect the mechanism underlying platinum resistance .
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PMC11593713
The study aimed to compare selected oxidative stress markers: total oxidative status (TOS), total antioxidant capacity (TAC), and the oxidative stress index (OSI) levels and glutathione peroxidase (GPx) and glutathione reductase (GR) activities in normal (MRC5 cell line) and tumor (A549 cell line) lung cells infected with human coronaviruses (HCoV) OC43 and 229E, human adenovirus type 5 (HAdV5), or human rhinovirus A (HRV A).
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PMC11063646
ANOVA for quadratic/linear models for drug release after 1 h and 24 h. Regression analysis of Spreadability, Extrudability, Viscosity, 1 h Drug release and 24 h Drug release.
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PMC11711063
ZG16B peptide fragments were verified in cell extracts by four unique spectra from mass spectrometry after transfection.
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PMC11715644
After epithelial cells formed monolayers on PDMS substrates and approached microchannel entrances, live time-lapse imaging was performed.
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PMC9739791
The small molecules TARF and FMN were the most efficient catalysts with the highest TOF values of about 20.
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PMC11397654
Although different families of calix[n]arenes are known, the calixarenes, macrocycles constituted of four phenol units connected through methylene bridges, have been more often employed as scaffolds because they are easily synthesised , and their conformations can be controlled by suitable substituents at the lower rim.
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PMC11481779
We have calculated the Pearson correlation coefficient and SCC for each example and Fréchet inception distance to compare the quality of the images.
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PMC9429973
HexaBody-CD38 is designed to induce strong anti-tumor activity in patients with CD38-expressing hematological malignancies through potent complement-dependent cytotoxicity (CDC) and other Fc-mediated effector functions.
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PMC4277423
While TZM.bl cells are widely used as target cells in HIV-1 neutralization assay, they are of epithelial origin .
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PMC9046263
Our most salient future studies will focus on elucidating the mechanism underlying HML-6 driven oncogenesis and the potential impact ERVK3-1 may have in this process.
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PMC9429973
E. Miles, R. Wali, C. Bryant, J. Patel, S. Nayani-Low, S. Moosajee Oral Surgery; Special Care Dentistry, King’s College Hospital, London, United Kingdom Background: Significant numbers of haematology patients are seen on a clinic dedicated to the management of patients with medically complex backgrounds within the Dental Institute of King’s College Hospital.
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PMC11694066
This identified several DNA repair inhibitors as being highly toxic to the JN1 cell line, including three clinical PARPi (talazoparib, olaparib, and rucaparib, ranked #6, #7, and #9, with Z-scores of −2.018, −1.828, and −1.762, respectively) and one ataxia telangiectasia mutated inhibitor (ATMi)/ATRi (KU60019, ranked #18; Z-score, −1.2225).
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PMC11706776
In E, G, boxes represent the interquartile range, upper and lower whiskers the largest and smallest values, respectively.
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PMC9712534
The t1/2 of fluorescence recovery was calculated to be 9.54 ± 3.56 s (DMSO) and 8.21 ± 1.98 s (ICRF).
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PMC9429973
Methods: An online platform (EMPATHY) for systematic monitoring of patient-reported AEs (including a set 22 items mainly derived from the PRO-CTCAE Item Library) was developed for the purpose of this study.
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PMC9429973
Results: Frailty status was generally proportional between arms, with 27% and 35% classified as fit, 43% and 36% int, 25% and 27% frail, and 5% and 2% unknown for KdD and Kd, respectively.
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PMC9111184
Fig. 3Second derivative of FTIR spectra of Control and m-CA, ChA, RA, EZ treated cells in the spectral range 1300–1000 ​cmFig.
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PMC11564405
The study methodology was rigorously chosen, and all data content followed the pathological classification parameters established by the World Health Organization for lung cancer.
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PMC10933092
A, B) Venn diagram showing the numbers of mA modification sites identified in mRNAs and lncRNAs from short hairpin-negative control (sh-NC) and sh-TRMT61A 5637 cells. (
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PMC9429973
H. Fogarty, M. Sardana, L. Sheridan, P. Chieng, S. Kelly, N. Ngwenya, C. Sheehan, K. Morris, E. Tuohy Irish Centre for Vascular Biology, Royal College of Surgeons in Ireland; Department of Haematology, St James’s Hospital; School of Medicine; School of Pharmacy, Royal College of Surgeons in Ireland; Irish Blood Transfusion Service, Dublin, Ireland Background: Blood donors of minority ethnicity, especially those of African origin, are under-represented in many high-income Western countries, including in Ireland.
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PMC11291697
B HPLC dosage of glycine, D- and L-serine and D- and L-aspartate in LCL, cell culture supernatant, and blood serum collected from the proband and twelve age and gender-matched unrelated healthy individuals as controls.
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PMC8389560
To simultaneously monitor apoptotic and healthy cells after SeNP treatment with fluorescence microscope, Apoptosis/Necrosis Detection Kit was used.
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PMC9031474
IC50 is the drug concentration causing 50% inhibition of the desired activity and is primarily applied for a specific target.
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PMC11612794
The basic motif of Dsn1 is shown as a cartoon.(B) Schematic representation of chicken Dsn1 (349 aa).
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PMC9429973
One of the splice defects located in ANK1 and the nonsense variant in SPTB were de novo variants.
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PMC10778532
Based on these findings, ZHX2 has been identified as a potential new therapeutic target for ccRCC patients .
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PMC10565698
5 Effect of miR-211-5p on tumorigenic properties of liver cancer cells.
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PMC2193049
The lysate was cleared by centrifugation at 12,000 g for 10 min.
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PMC11345828
In the clinic, however, compounds 1, 4, and 5 have reported dose limiting toxicities, including increases in patient troponin I levels, a possible indicator of cardiac injury.
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PMC9429973
We performed functional imaging in 78 patients with either FDG positron emission tomography or diffusion-weighted magnetic resonance imaging within a median of 1 day after NGF assessment.
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PMC7351993
Highly lipid-rich, but rare (only 0.3% of the total population) Chlamydomonas sp. mutants (indicated by the yellow region) were sorted by the RIACS.
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PMC11697189
Differential expression of HSP genes was observed in ccRCC and normal tissue.
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PMC11441402
Figure 1 depicts full well imaging and viability-dye based analysis of wells using the integrated software of the digital automated microscopy cell imaging system.
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PMC11064533
In case of ampicillin exposure, clumps produced by Δffp and ΔspnT were significantly (P.BH < 0.05) more resistant and protected the bacterial clumps, with ΔspnT showing the highest number of viable bacteria in terms of relative adhesion as exhibited in Fig. 5A.
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PMC10495912
SOD enzyme activity The SOD enzyme activity in HepG2 cells after exposure to a concentration of 75 µM of TQ significantly increased compared to the control group (p < 0.001) and the cells exposed to hydrogen peroxide alone (p < 0.001).
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PMC11519788
Subsequently, protein A agarose was added for 1 h to capture the antibody-protein complex, followed by 5 M NaCl crosslinks reversion and DNA extraction for qPCR analysis.
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PMC10142392
If we examine the production of scientific research regarding PCL applications, we can find that PCL-based materials are increasingly being used as specific drug release vectors in cell culture, regenerative medicine implants, and drug release .
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PMC11019355
To understand mechanisms, they next performed interspecies MPRAs, transfecting the human ATAC-STARR-seq library into the rhesus macaque cell line (HM) and vice versa (MH).
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PMC11782508
The tree was constructed with MEGA11.0, and the genetic distances were computed using Kimura’s 2-parameter model.
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PMC11172027
The induction of apoptosis in cancer cells occurs through selenium-containing glutathione.
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PMC8658661
Thus, the human cell lines THLE-2 and THLE-3, considered models of normal hepatocytes, are controversial.
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PMC10452486
Further inhibition experiments carried out with the peptide sequences on the binding of mAbs MEM-E02, E07, and E08 to HCs of different HLA loci coated on solid matrix (LABScreen beadsets) revealed the sequences (QFAYDGKDY and DTAAQI) and inhibited the binding of the mAbs in a dosimetric manner (Figure 3A–C in ).
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PMC11319300
We found that the entire MYEOV locus, including the homologous MYEOV-3′-putative enhancer region, is covered by strong enhancer or enhancer/promoter domains in the four non-human primates (Figure 4).
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PMC9429973
It illustrated that MET technology can be applied on multiple kinds of tumor cells.
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PMC9429973
Median time to response was 7.9 weeks.
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PMC11360263
Human cytomegalovirus (HCMV) infection is common in tumor tissues across different types of cancer.
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PMC9429973
Data were collected from the medical files of patients, who filled out at least two PedsQL questionnaires between 2012 and 2021.
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PMC11525028
RT-qPCR and immunoblotting showed that the mRNA and protein levels of ASNS were decreased in NAT10-KO group (Figures 4C and S2A).
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PMC11640555
The GBM cells are characterized by rapid growth and the invasion of adjacent brain regions .
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PMC11564322
p < 0.05; **p < 0.01; ***p < 0.001 vs. the vehicle control. ###
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PMC11707832
Lysosomal changes and dysfunction have been identified in a wide variety of diseases, including autoimmune, metabolic and kidney diseases, PD, diabetes mellitus and lysosomal storage diseases .
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PMC11711663
To ensure accurate feature calculation, we standardized the voxel spacing across patients by resampling CT images and applied gray-level normalization to enhance feature comparability and robustness across different settings.
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PMC11531735
MDA MB 231-BrHER2+GFP-Luc; 6.
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PMC11389534
In addition, UFMylation plays critical roles in innate immunity depending on the stimuli and cellular background.
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PMC7642379
The black line in each box shows median of normalized counts for the gene. (
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PMC9621239
This study included 24 HCC patients.
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PMC5363531
The effect of SRC dominant negative was evident also at the protein level, reducing endogenous TS protein (Figure 3C).
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PMC9429973
To this end, Diffusion-Weighted Magnetic Resonance Imaging (DW-MRI) and bone turnover markers are promising radiological and serum metrics, respectively, although their clinical value in prospective assessment is unclear.
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PMC11377827
CA9: control vs. USP43 sgRNA 1% O2 **P = 0.0086.
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PMC11629461
Student's t test was used for comparison between Light (E) and Light + VAS2870 (G), asterisk (*) indicates statistical significance at p < 0.05.
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PMC9220170
According to the results of this test, shown in Figure 3, we can speak about the absence of this knockdown effect of each of the selenoproteins on the growth of cancer cells without the additional influence of external stimuli on soft agar.
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PMC11538390
Note that this A corresponds to the T allele of rs570516915 on the opposite DNA strand.
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PMC11036064
Lyustikman et al. have reported that RAF1 is upregulated in human GBM tissues and RAF1 activation interacts with AKT to develop GBM; the histological feature of RAF1-induced GBM has been comparable to KRas-induced GBM (Lyustikman et al., 2008).
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PMC9429973
Aims: The aim of this study is to analyze demographic and clinical characteristics, laboratory parameters, the presence of comorbidities, laboratory parameters, disease status, as well as outcome of the patients with COVID-19 and lymphoproliferative disease and compare them with characteristics of covid-19 infection in patients from general population (GP).
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PMC8875242
In keeping with this hypothesis, rilpivirine did not affect digoxin efflux in hPCIS (Figure 2A).
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PMC11637276
Confocal experiments were analysed manually.
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PMC11746948
Statistical significance between groups was determined by Student’s t-test, one-way analysis of variance (ANOVA), or two-way ANOVA. *
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PMC9516447
Interestingly, in the lung cancer cell line H1299 harboring mutant NRAS, HA15 or YUM70 substantially reduced the mutant NRAS protein level (Supp.
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PMC11098378
In contrast, incubation with GST-IpaH2.5 did not result in ubiquitination of either protein (Fig 5E and 5F).
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PMC9429973
Methods: CCP was collected from volunteer donors in two time periods (September 2020-January 2021 [early period] and April- May 2021 [late period]).
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PMC11476004
The genetic assays are very sensitive and can be used as biomarkers of normal DNA replication and repair potential and the maintenance of control over the entire cell cycle.
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PMC11754094
PE systems that use ngRNA or the stabilized epegRNA showed particularly high frequencies of large deletion.
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PMC11794847
.
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PMC9429973
On multivariate analysis, previous ibrutinib therapy retained its significance and lowered the risk of RT.
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PMC6034753
While Meq expression was restricted almost exclusively to the nucleus, apoptin was distributed both in the nucleus and the cytoplasm of CEF (Figure 1A).
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PMC11055443
Probably, the PGE2 reduction will be effective in inhibiting inflammatory responses and epithelial barrier destruction.
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