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OTAR3088
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PMC9412887
Therefore, Lin et al. constructed a PTEN mRNA nanocarrier (mPTEN@NPs) delivered to tumor cells with PTEN deletion or mutation to restore the immunosuppressive TME, stimulate immune response and improve the efficacy of immune checkpoint blockade (ICB) therapy by inducing autophagy activation and damage-associated molecular patterns (DAMPs) release.
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PMC11726848
After irradiation, 30 μL of cell suspension (≈0.1–1 × 10 cells/mL) was mixed at 25 °C with an equal volume of 1% low-melting agarose (LMA) solution prepared in PbEDTA.
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PMC11781181
However, other forms of cell death involved in this process remains to be elucidated.
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PMC9019892
In addition, it also exhibited dose‐dependent inhibition of ERK phosphorylation upon FGF ligand stimulation.
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PMC11507371
Pasqual-Melo et al. reinforced the dose-dependent decline in metabolic activity of A431 and HaCaT cells 24 h after CAP exposure.
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PMC9599726
RNA quality was checked with a fragment analyzer (Agilent Technologies).
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PMC11643491
Beyond these professional phagocytes, many other cell types whose primary function is not phagocytosis (e.g., epithelial, endothelial, fibroblast, cancer, and mesenchymal cells) can also engage in particle uptake through a phagocytic-like process .
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PMC9844987
Micrococcal nuclease-digested nucleosome sequencing (MNase-seq) data from 7 EBV-immortalized LCLs (GM18507, GM18508, GM18516, GM18522, GM19193, GM19238, GM19239) were obtained from GEO (GSE36979) (Gaffney et al., 2012).
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PMC5034105
Although most patients infected with ZIKV have an asymptomatic or a self-limiting acute febrile illness, some patients may develop severe neurological complications, such as Guillain–Barré syndrome, or fatal disseminated infections.
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PMC11803149
In our scRNA-seq data, exhaustion-related genes Pdcd1 and Tox were highly expressed in cells among clusters 0 to 4, but not in cluster 5 ( Figure 4B ).
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PMC9429973
Additional analyses of pts with BL PLT <100 x 10/L (N=100) and BL PLT <50 x 10/L (N=31) show similar treatment effects of MMB vs DAN.
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PMC10253553
The 786-0 cells were purchased from ATCC by the corresponding author and used within 15 passages.
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PMC7823217
As mentioned above, it has been reported that a subtype of glial stem cells within the tumor are able to trans-differentiate into endothelial cells promoting angiogenesis , therefore utilizing these patient-derived cancer cells may establish a vasculature in bioprinted cancer construct in the presence of adequate tumor microenvironment.
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PMC10988555
Several in vitro studies suggest that cobalt complexes may become promising anticancer agents.
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PMC11758416
Passaging them every 3–4 days depending on the confluency of cells is ideal and will help prevent background noise in your experiment.
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PMC11766309
P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns—not statistically significant.
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PMC11240571
Programmed death receptor 1 (PD-1) is a receptor on T cells that sends a suppressive signal that inhibits T cell anti-neoplastic activity by binding to PD-L1 .
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PMC9429973
Overall survival (OS) at 1, 3, and 5 years were 69.7%, 54.9% and 42.5% respectively.
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PMC11585565
The estimated half-life of p.(Gly492Cys), p.(Gly492Asp) and p.(Thr495Ile) CNNM4 proteins was 0.61 h (R = 0.89), 1.18 h (R = 0.90), and 12.11 h (R = 0.61), respectively, whereas the WT CNNM4 protein data was insufficient to fit the equation (Fig. 3f).
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PMC11300639
According to reports, RNF26 limits the response to type I interferon by promoting the autophagic degradation of IRF3.
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PMC5034105
Table 2).
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PMC9118379
Identification results of three de novo sequencing tools based on the top 10 hits of each of the 320 spectra. (
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PMC9429973
Of these 22 patients, 11 died during active first-line treatment due to progression of myeloma or treatment-related toxicity.
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PMC11552389
Tumors with FOXN3 over-expression had a significantly lower percentage of Ki67-positive cells compared to tumors without over-expression (Figure 3D).
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PMC11676670
Routine mycoplasma testing confirmed the absence of contamination throughout cultivation.
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PMC11794568
RT-qPCR (A) and WB (B) confirmed that CRIP1 was stably overexpressed in SK-MEL-2 and A375 cells.
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PMC10222971
Additionally, owing to the difficulties in clinical trials, there is no FDA (Food and Drug Administration) registered topical treatments of skin cancer so far .
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PMC7723249
However, MDCK-1 is not the most appropriate model to study the molecular pathogenesis of CCHFV since dogs are genetically distant from human and no clinical signs of CCHF have ever been described in infected-dogs.
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PMC8009078
Throughout the simulation, a constant temperature of 300 K was retained using the Langevin thermostat with a collision frequency of 2 ps-1, at 1 atm using a Monte Carlo barostat with volume exchange attempts every 100 fs.
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PMC11705630
Expressions of interferon regulatory factor 9 (IRF9) and signal transducer and activator of transcription 2 (STAT2) were elevated in GLDC knock-downed cells and decreased in GLDC over-expressed cells.
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PMC11680391
This communication strategy represents a positive shift toward more effective collaboration, where the veterinarian’s guidance is paired with a clear roadmap that drives accountability and tangible outcomes.
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PMC10654497
Ten SFRGs were identified. (
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PMC9817179
Simulation of the fixation artifact as a function of the starting punctate percentage and the relative overall fixation rate , assuming individual fixation rates are constant (, ).
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PMC11791432
The screening process was shown in supplementary figure 1.
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PMC11798926
However, the possibility remains that even though SynDIG1 interacts with μ2 and μ1a, some other molecular machinery which interacts with residues 161–76 could control trafficking to and from the TGN.
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PMC7504302
Accordingly, their expression is frequently deregulated in carcinogenesis .
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PMC11637276
Cells engage with the ECM through the heterodimeric integrin receptors, which are composed of an α and a β subunit and activate diverse pro-survival signal transduction pathways .
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PMC10243817
CRSwNP was further classified as eosinophilic when the percentage of tissue eosinophils exceeded 10% of the total number of infiltrating cells, as reported by our previous study (18).
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PMC11577421
Characterization of BSA, BSA+, and BSA–. (A) Electrostatic surface potential of native BSA (total charge −15 e), BSA+ (total charge +20 e) and BSA– (total charge −40 e). (
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PMC11026382
These measurements were made for DHFR mutations G121V, F31Y/L54I, M42F/G121V, F31Y/G121V and the WT in the background of WT TYMS and TYMS R166Q.
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PMC9429973
M retreatment had a manageable safety profile, consistent with the initial treatment.
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PMC9429973
Our findings support the use of therapeutics that increase Hb in patients with SCD to protect against deleterious organ damage.
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PMC11045125
Additional variant annotations were added from Clinvar using SnpSift .
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PMC9429973
Immunophenotypic analysis of CAR T cells was performed by FCM.
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PMC11467964
PRF1. *
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PMC8728081
Rasasindura is a mercury-based medicine that is used to treat high fever, jaundice, sexual diseases, immune and nervous system related diseases .
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PMC7987994
Extraction volume is one of the most important parameters.
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PMC11720808
Corresponding secondary antibodies were supplied by ZSGB Biological Technology Co. Ltd. (Beijing, China).
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PMC6684588
n = 7–11). (
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PMC10024132
The off-the-shelf nature of blinatumomab makes it a ready-to-use product, whereas autologous CAR-T manufacture may require weeks.
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PMC8409415
CD70 expression was confirmed in A2780cisR, SKOV3, and SKOV3cisR cells.
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PMC9352806
This study can enrich the mechanism of the biological activity of bitter melon saponins, and provide potential applications in the field of food nutrition.
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PMC10729469
ARY006, R&D systems, Minneapolis, MN) following manufacturer’s instructions.
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PMC9621239
In addition, the pRL-TK vector, which expresses Renilla luciferase, was also co-transfected to correct the differences in both transfection and harvest efficiencies.
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PMC11607321
The synthetically enhanced data increases statistical power, uncovering less studied mechanisms associated with drug resistance, and refines the identification of genetic associations and clustering of cancer cell lines.
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PMC11587541
Telomere shortening is a natural process that occurs with each cell division.
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PMC11590005
Univariate analysis and Poisson regression with robust variance were applied.
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PMC11549612
This observational study was carried out at Prince Sattam bin Abdulaziz University, Al-Kharj, Saudi Arabia, from January to November 2023.
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PMC9878278
SC were co-transfected with siMDA5, and siRIG-1 (exon 11), and knockdown was determined by measuring mRNA transcripts. (
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PMC7039683
Bar chart showing number of embryos positive for GFP signal in the periderm after being injected with the intact reporter or one in which the GRHL motif was deleted. (
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PMC5673060
Here we utilise these two approaches to determine the signalling pathways which are enriched in pazopanib- and dasatinib-resistant cells and uncover a new vulnerability to the HSP90 inhibitor NVP-AUY-922 which has utility in overcoming acquired resistance to these TKIs.
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PMC8097906
Notably, VSM exposure revealed low cytotoxicity in heathy breast and bone cells, as well as human mesenchymal stem cells (Fig. 6a).
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PMC11495567
PCAIs with large N-cycloalkyl substituents as in NSL-YHJ-2-27 with a cyclooctyl moiety were more effective than those with smaller rings as in NSL-YHJ-2-48 with a cyclopropyl group.
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PMC11594641
The proliferation of RAB27A knockout (KO) SkMel28 cells was inhibited compared to the wild type (WT).
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PMC11670407
As shown in a Venn diagram, compared with the anti-IgG antibody, the anti-OTULIN antibody yielded 496 proteins, including the anti-lipid peroxidation protein GPX4 (see Supplementary File 2, Sheet 2) (Fig. 4b).
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PMC11545737
Caspase-1 is required for the processing of pro-IL-1β but was dispensable for cell death which instead was found to be dependent on NLRP3 and cathepsin B .
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PMC9429973
In brief, fetal liver-derived stem cells, isolated from STAT3α and STAT3β deficient mice on C57BL/6J background, were transduced with a retrovirus introducing the human fusion-oncogene MLL-AF9, and intravenously transplanted into immunocompromised mice.
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PMC11371627
Symptomatic therapy has not been necessary according to our patient.
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PMC7433824
The paradox on the effect of low and high doses of P4 on GBM growth is gaining attention.
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PMC8427838
Cells expressing TMIGD1 alone or together with moesin were seeded onto coverslips and grown overnight in 60-mm plates to 80–100% confluence.
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PMC11754094
Our findings show that the present genome-editing tools need additional validation to ensure that large-deletion events are not present, or that these tools need additional engineering to prevent the generation of DSBs and large deletions.
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PMC11750161
Full-length fragments were cleaved using the XhoI and EcoRI restriction enzymes and ligated into the pCORON SP VSVG mammalian expression vector.
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PMC8567602
Although the position of BAF-family peaks with respect to genes was similar between fusion-only and combination genotype tumors, there was an increased prevalence of intronic and decreased prevalence of promoter BAF-family binding sites in Smarcb1-loss–alone tumors (Fig. 3B; Supplementary Fig. S3C).
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PMC11367146
are some MF that involves highest number of target genes.
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PMC8348645
Standard gallic acid quantification was done as presented in Figure 4 .
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PMC11656048
PyMOL2.3.0 software was adopted to remove protein crystalline water, primitive ligands.
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PMC9812014
regel (family Amaryllidaceae) showed anti-PV activity in vitro.
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PMC5261804
Of the 7814 PPIs examined, 6503 (83%) had a PPI-MPER > 1 in at least one cell line.
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PMC11348300
However, given the ample photon budget provided by our intensified camera, prioritizing deeper tissue penetration was considered more advantageous.
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PMC9429973
Also the study shows that 3’-UTR SNPs of DICER1, DROSHA have more potential to impact MDS progression than coding variants.
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PMC10040136
After silencing receptor II in the TGF-β receptor complex on the surface of GIST cells (C), both conditioned medium and recombinant TGF-β2 protein failed to activate the PI3K-AKT pathway of GIST (D, G).
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PMC11499381
Each data point represents an independent experiment, with lines denoting the mean ± s.e.m.
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PMC9429973
With the persistence of COVID-19 and the absence of antibodies to COVID-19, 2 patients received repeated transfusion of human IG against COVID-19.
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PMC11486946
During the imaging, autofocusing was continuously running, and the ligand was dispensed at desired times by robotics.
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PMC9429973
125 patients had acute chest syndrome and 210 had vaso-occlusive crisis (VOC), of whom 74 had >3 VOC in the last 12 months.
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PMC10047551
For all cells, the culture medium was supplemented with 10% fetal calf serum, 1% penicillin-streptomycin, 10 mM HEPES, and 1 mM sodium pyruvate.
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PMC9429973
FISH was not carried out.
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PMC9429973
In the non-transplantation group, 78.9% of patients achieved VGPR or better after 4 courses of induction therapy, 44.7% of the patients achieved deep response and the rate of undetectable MRD was 42.4%.
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PMC10551595
Next, 10 μL of MTT mixture (5 mg/mL) (Atocel) was added to each well following the growth curve‐based incubation times (96 h for NALM6; 72 h for CCRF‐CEM, RCCRF‐CEM, RN95; 48 h for patients primary cells; 24 h for MNCs).
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PMC11585254
Data represent the mean ± S.D. from three independent experiments.
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PMC11653552
This neoplasm is responsible for 10-15% of all hematologic cancers and accounts for approximately 20% of cancer-related deaths.
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PMC11658074
f, g Quantification of the melanin content and skin elasticity in the control group and fat graft group.
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PMC11612626
ONX-0912 (oprozomib, PR-047) is an orally bioavailable inhibitor for ChT-L activity of 20S immunoproteasome β5i subunit.
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PMC11509278
The extraction procedure for EEP was modified .
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PMC9429973
SCR can be divided into non-proliferative sickle cell retinopathy (NPSCR) and proliferative sickle cell retinopathy (PSCR).
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PMC9000591
A plant-based product needs to be qualified in all evaluations to demonstrate that its benefits outweigh its risks before the product can be widely accepted as a drug .
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PMC11596967
A representative species of this genus, Deinococcus radiodurans (D. radiodurans), is distinguished by its rapid and efficient DNA repair mechanisms .
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PMC9429973
Up to 130 patients (30 in Phase 1a, 100 in Phase 1b) will be enrolled and treated until disease progression or unacceptable toxicity.
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PMC11708541
Antibiotics are a class of antimicrobials specially used to contest bacterial infections.
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PMC11552389
The mice were given subcutaneous injections of either 2 × 10 A375-vector cells or 2 × 10 A375-FOXN3 cells suspended in 100 μL PBS.
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