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PMC11555465
Following the treatment, 10 µl CCK-8 solution was added to each well and cells were incubated for 1 h, according to the manufacturer's protocol.
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PMC11521786
Prior to their addition to the cells, the sera were incubated at 56°C for 30 min to inactivate the complement.
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PMC4685407
Gene targeting is based on homologous recombination using sequences targeting a specific genomic region of the host cell.
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PMC11731161
As shown in Fig. 6B, knockdown of USP7 significantly enhanced the cell growth inhibition induced by SEL.
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PMC8526701
After 7 days, mice were treated with 10 mg/kg GSK602 or the vehicle control for 10 consecutive days.
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PMC10178190
Increased cell viability of A2780CisR cells treated with RJY13 in presence of human MSC-derived CM was accompanied by reduced levels of apoptosis, as monitored by the decrease in cleaved PARP (cPARP) levels (Figure 1B,C).
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PMC10965315
MTT assay for determining cell viability.
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PMC11401358
The results showed that the high fluorescence intensity of ThT appeared at 495 nm in the (GAGSGA)2-8 h group.
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PMC10335954
GSEA was attempted to compare the dataset with under- and overexpression of TRIP13, finding that the JAK/STAT and p53 pathways may regulate differential TRIP13 expression.
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PMC11787381
Effects on survival of microtubule-associated protein 2 (MAP-2) neurons (A) (control (white) = 38 ± 1 MAP-2+ cells/field), neurite network (B) (control = 389 ± 13 µM), and phosphorylation of Tau (AT100) (C) (control = 469 ± 13 µM/MAP-2+ cell.
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PMC10993311
T3 = 30-36 weeks and T4 = delivery.
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PMC9065483
The lead contact for this project who will be responsible for distribution of materials, datasets, and protocols used in the manuscript is Boaz Wong (boaz.wong@uottawa.ca).
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PMC9429973
T cells were activated by antiCD3/CD28 beads and transduced with antiCD19 CAR-T, LV or FV vectors.
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PMC4277423
One deletion/insertion mutant had −1 nucleotide, +2 nucleotides and another −7 nucleotides.
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PMC10291556
Statistical significance from untreated control was determined with the Student t-test (*p ≤ 0.05, **p ≤ 0.01, and p ≤ 0.001). (
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PMC10810426
Significance was determined by 1-way ANOVA. **
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PMC11252033
Such complementary approaches demonstrated ASA interacting within the nicotinamide site of the NADPH binding region of CryZ, that was found by FTMap and FTSite as a strong main hot spot.
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PMC11267830
Evidence derived from publicly available datasets robustly demonstrates that numerous lymphoma and leukemia cell line models show pronounced sensitivity to the therapeutic agent indisulam .
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PMC9429973
Interestingly, Western blot analysis of Brusatol-sensitive cell lines showed decreased protein levels of pro-survival apoptosis-regulating proteins Bcl-2, Bcl-XL, and Mcl-1.
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PMC11310713
In this study, we applied micro-sized ovarian cell-laden hydrogel beads as an approach to cell-based HRT using a minimally invasive method in the menopausal rat model.
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PMC11347429
Principal component analysis and gene expression by genotype. (
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PMC11228511
Mean ± SEM. *
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PMC11478724
Additionally, PMX had the strongest effect on malignant mesothelioma cells.
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PMC11412752
HepG2 is an immortal cell line derived from a hepatocellular carcinoma patient, and chromosomal instability (CIN) is a hallmark of human cancer .
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PMC11472569
Emerging evidence indicates that EVs play a crucial role in developing drug resistance.
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PMC11637820
To understand the efficacy of CAP on biofilms, in vitro assays were performed.
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PMC11679033
The lack of significant LDH release suggests that cytotoxic effects were not due to direct membrane disruption but likely involved intracellular pathways.
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PMC9429973
As the origin of all immune cells, hematopoietic stem cells (HSCs) undoubtedly play a critical role in the inflammatory response.
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PMC10870498
Representative visual fields were acquired using a Leica DM5000 B microscope (Leica Microsystems, Wetzlar, Germany).
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PMC10158546
Normalized protein expression of PrP, TUBB3 and GFAP in ReN WT and 129M cells throughout standard differentiation.
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PMC9429973
Detailed analysis regarding causes of death and risk factors to be presented at the meeting).
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PMC11306331
MBP-induced condensate dissolution thus appears to act nonlinearly, with a large increase in condensate dissolution observed over a narrow range of concentrations.
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PMC9065483
This effect occurred within a broad drug treatment range leading to no more than a 25% decrease in cell viability (Figure 4C).Figure 4Vanadate promotes proinflammatory cytokine production through EGFR-NFκB activation(A) Previously acquired microarray data published by Selman et al. was analyzed using a computational script that selected genes that were of increased or decreased abundance 3-fold upon vanadate treatment from the infected only control.
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PMC8973725
Recent studies have demonstrated that zinc-finger protein 677 (ZNF677) acts as a tumor suppressor gene in cancer.
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PMC8903741
After 48 h, the cells were collected by trypsin treatment and the transfection efficiency was estimated by imaging the EGFP fluorescence using fluorescence microscope (Olympus, Tokyo, Japan).
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PMC9429973
Treatment with asparaginase is associated with a plethora of adverse effects, amongst which the occurrence of hypersensitivity is one of the most common.
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PMC11568601
However, cells transfected with IRE1-OE plasmid were screened with 200 µg/mL G-418 disulfate (MCE, USA), and stably transfected cells were utilized for western blot experiment.
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PMC11756907
The following reagents were used in this procedure: graphite with flake sizes from 40 to 150 μm (Asbury Carbons USA, catalogue number 635), sulfuric acid 96% (Chempur) 750 mL, sodium nitrate (Chempur) 16.5 g, potassium permanganate (Chempur) 90 g, hydrogen peroxide 30% (Chempur) 30 mL, hydrochloric acid 3% (Chempur).
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PMC11592008
The NLRP1 (nucleotide-binding domain, leucine-rich-containing family, pyrin domain-containing-1) inflammasome is the most important inflammasome in human keratinocytes.
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PMC9429973
Methods: Individual patient-level data (IPD) in ELARA (tisa-cel; NCT03568461; 03/2021 data-cut; N=97 [infused set]; N=90 [efficacy-evaluable set]) were weighted to match the patient population in ZUMA-5 (axi-cel; NCT03105336; 03/2020 data-cut; N=124 [infused set]; N=84 [efficacy-evaluable set]).
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PMC11736898
Keloids are characterized by abnormal and excessive deposition of extracellular matrix (ECM), particularly type I collagen, which may result from an imbalance of ECM production and degradation, in the context of a protracted increase in inflammation post-healing .
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PMC3019555
Collectively, these findings indicate that efficient drug–tubulin interaction is required for the inhibition of HIF-1α translation.
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PMC6889484
Insertion of the 5′ direct repeat fragment of A3G-D128K (nt 1 to 1,083) followed by an in-frame insertion of P2A fused to eYFP lacking a start codon created vector pA3dr(DK).
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PMC10213179
Twenty-four hours after plating, cells were washed with serum-free medium and incubated with 100 μg/mL yeast tRNA in serum-free medium at 37°C for 15 min (blocking step).
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PMC9622879
This is because, in the community with the proposed CEMS, the objective function minimizes the overall energy cost of the community.
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PMC3019555
Although we arbitrarily used a cutoff of three P-bodies per cell, many MTD-treated cells contained >40 P-bodies.
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PMC11531744
Our findings provided a theoretical basis for developing prognostic model constructed by IRGs clustering subgroups, predicting patient prognosis and selecting patients for combination therapeutic drugs.
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PMC11612626
B, Schematic of the HTS using the cell-permeable fluorogenic substrate LLVY-R110 that is specifically cleaved by proteasomes and immunoproteasomes.
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PMC11726848
The slides were placed in a lysis solution (2.5 M NaCl, 10 mM Tris-HCl, 1% Triton X-100, 1% N-lauryl-sarcosine, and 100 mM EDTA; pH = 10.0) for 25 min at 37 °C in the dark.
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PMC9895440
n = 6 donor replicates of n = 2 experiments.
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PMC11723947
To identify the cellular functions regulated by HP1α, we then analyzed T cell programming ex vivo by flow cytometry.
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PMC11727062
We have limited our scope to peer-reviewed articles published in Chinese or English over the preceding decade.
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PMC9368503
In line with this observation, previous studies have shown that the induction of EMT drives doxorubicin resistance in multiple cancer types .
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PMC11306331
n = 26,22,24,23,28,26 cells from conditions labeled left to right, pooled from three biological replicates.
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PMC8348645
Test for Saponins: Test for Saponins (Foam test): To 0.2 mL of extract was added 0.6 mL of water in a test tube.
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PMC6034753
It has also been shown that DNA damage response induces nuclear re-localization of apoptin in primary cells .
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PMC9429973
Efficacy endpoints included hemolytic markers and the Functional Assessment of Chronic Illness Therapy-Fatigue (FACIT-Fatigue) as a measure of QOL.
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PMC9532503
Common cancer treatment options include chemotherapy, radiotherapy, surgery, and in some cases a joint therapy has shown encouraging results .
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PMC11720808
Analysis of mRNA levels of indicated genes from the groups treated with DMSO and SCU (100 μM) for 48 h. GAPDH served as the loading control. (
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PMC5173112
We then induced depletion of USP21 using siRNA in RCC cell lines (including 786-O and A-704), which significantly represses the growth, invasion ability and CSCs population of RCC cell lines.
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PMC11046454
To construct Apt-CAR T cells, an anti-FITC (fluorescein isothiocyanate)-E2 scFv fragment was fused with a second-generation CAR containing a CD8α hinge, a CD8α transmembrane (TM) fragment, a 4–1BB costimulatory fragment, and a CD3ζ activation fragment in the intracellular domain (Figure S1 of the Supporting Information).
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PMC11496491
GO.IDTermGO:0005515Protein bindingGO:0043167Ion bindingGO:0019904Protein domain specific bindingGO:0043169Cation bindingGO:0046872Metal ion bindingGO:0043168Anion bindingGO:0005488BindingGO:0019864IgG bindingGO:0016740transferase activityGO:0000166Nucleotide bindingGO:1901265Nucleoside phosphate bindingGO:0019787Ubiquitin-like protein transferase activityGO:0005524ATP bindingGO:00515394 Iron, 4 sulfur cluster bindingGO:0035639Purine ribonucleoside triphosphate bindingGO:0070016Armadillo repeat domain bindingGO:0036094Small molecule bindingGO:0008092Cytoskeletal protein bindingGO:0004842Ubiquitin-protein transferase activityGO:0070300Phosphatidic acid bindingFig.
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PMC6133382
FIA details are provided in the Materials and methods section.
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PMC10092581
On the contrary, GR activity was only very minimally affected by tested complexes.
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PMC11562028
The blood index analysis included quantification of hemoglobin (HGB), hemoglobin content (MCH), lymphocytes (LYM), lymphocyte ratio (LYM%), intermediate cells (MID), intermediate cell ratio (MID%), total number of red blood cells (RBC), mean volume of red blood cells (MCV), red blood cell distribution width SD (RDW-SD), red blood cell distribution width CV (RDW-CV), granulocytes (GRAN), granulocyte ratio (GRAN%), total white blood cell count (WBC), total platelet count (PLT), mean platelet volume (MPV), platelet distribution width (PDW), platelet hematocrit (PCT), and platelet large cell ratio (P-LCP).
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PMC9429973
Median duration of post-FEDR follow-up was 5.1 months (IQR, 3.0–17.3) for patients initiated at FEDR 400 mg daily and 4.4 months (IQR, 3.0–14.3) for those initiating at <400 mg daily.
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PMC11024707
In postmortem brain mi-RNA research, mi-RNA-23a expression levels were reported to increase in individuals with ASD .
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PMC11670407
Equal masses of protein were subjected to SDS-PAGE gel electrophoresis and transferred to PVDF membranes (Millipore, USA).
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PMC11806818
CTCs are released into the bloodstream from primary tumors or metastatic circulating cancer cells that may display overall tumor characteristics, and are used as markers reflecting biological and clinical aspects of liquid biopsy markers, thus facilitating non-invasive real-time monitoring of cancer patients .
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PMC9429973
This calls for more thorough screening of these drugs, incorporation of information on mutagenicity to healthy cells in drug safety guidelines and patient surveillance over time.
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PMC9429973
At the same level of guanosine in the liver, forodesine promoted TNF-a production from in vitro alveolar macrophage cultures.
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PMC5330095
The benefit of CBD in alleviating liver fibrosis, which is one of the outcomes of untreated viral hepatitis, was also demonstrated in previous studies.
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PMC11643491
Statistical data and error were analyzed on GraphPad Prism 9 (www.graphpad.com (accessed on 21 November 2024), San Diego, CA, USA).
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PMC11237030
AQP5 is a transmembrane protein, thus the expression in its native form as soluble recombinant protein is very difficult to achieve.
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PMC9429973
The study results were comparable or even favorable compared to those of major clinical trials, in which one-size-fits-all strategies were adopted.
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PMC11791432
At the same time, we explored differences in ANXA1 expression levels in different molecular subtypes of gliomas.
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PMC11461833
Previous studies have shown that although VAMP-2-null mice die immediately after birth, VAMP-2-heterozygous-null mice exhibit improved motor coordination.
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PMC11489175
When the bond of the ubiquitin-DUB intermediate is hydrolyzed, substrate release occurs, and the CP DUB returns back to a thiolate reactivated DUB for a new enzymatic cycle 26,32.
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PMC11352311
After treatment, 2-DG solution was added to the wells following the manufacturer’s instructions, and the cells were incubated at 37 °C for 30 min.
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PMC9429973
As of December 4, 2020, median follow-up was 38.1 months and median treatment exposure was 17.5 months (range 0.1–65.3).
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PMC11678130
Similarly, Lori et al. demonstrated that the natural polyphenol morin sensitizes A375 CM cells to 5-FU by reducing its IC50 value in this cell line and improving its anticancer effect .
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PMC11599565
PAX8 is involved in the embryonic development of the thyroid gland, specifically its rudimentary lateral portion, which develops from neural crest cells.
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PMC6222726
Biological activities of hinokinin have been recently reviewed .
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PMC8623816
We mixed 8 µg of dsRNA from strain D122 with formaldehyde load dye (1:2) and incubated for 10 min at 65 °C to denature, and immediately placed on ice for 1 min.
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PMC11392912
Total 5 animal groups consisting of 6 animals per group were made: (1) untreated group/healthy control (HC), (2) Negative Control (NC) treated with a non-targeted miRNA pool, (3) CIA, (4) Clo-miR-14 treated group, and (5) standard drug (Methoxtrate) group.
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PMC11291697
The microdeletion involved exons 5-6 of GTDC1, encoding a putative glycosyltransferase, whose expression is particularly enriched in the nervous system.
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PMC11411131
This sensitivity issues can be attributed to the time delay necessary for the expression of Krab-Sec.
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PMC11566417
Certain bacterial pathogens, including MRSA, evade neutrophil phagocytosis by influencing their recruitment to the site of infection and/or by replicating inside host cells [111–114].
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PMC3765139
Singleplex PCR reactions were performed in Fast Gene Quantification in 96-Well Plates (the thermal cycling conditions included a step of 20 s at 95°C followed by 40 cycles of 95°C for 1 s and 60°C for 20 s) with The TaqMan® Fast Universal PCR Master Mix (10 μl) in a volume of 20 μl containing 2 μl of cDNA and 1 μl of specific TaqMan® Gene Expression Assay.
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PMC11786767
B: Methanol or acetone treatments reduce biotin-DHPE signals.
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PMC6133382
Examination of the gp120 subunit vaccines used in the RV144 trial showed that both components (MN-recombinant gp120 [rgp120] and A244-rgp120) were enriched for complex, sialic acid–containing glycans and lacked the high-mannose glycosylation found on the surface of virions and native envelope proteins required to bind the new class of glycan-dependent bN-mAbs [16–20].
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PMC11549062
Edu: 5‐ethynyl‐2′‐deoxyuridine. (
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PMC11767725
A case–control study investigated the connection between rosacea, insulin resistance, and MetS in 47 rosacea patients and 50 controls.
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PMC11075223
Therefore, both might be good candidate drugs to protect osteoblast damage and dysfunction induced by oxidative stress.
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PMC11803918
To investigate the combinational antitumor effect of γδ-T exosomes and PDT, A375 cells were incubated with liposomes, γδ-T exosomes, and hybrid exosomes with or without light irradiation (Figure 4E,F).
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PMC6934009
In the present study, the levels of ROS were markedly promoted in A549 and A427 cells with wogonin.
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PMC10158546
ReN cells were treated with 1 μL of mouse PrP expressing lentiviral prep (LV-prep) for 72 hours, with or without TransDux enhancer, at which point the cells were treated with 0.5 μg/mL puromycin.
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PMC11588085
XIST’s role in ovarian cancer has only been explored in the context of therapeutic resistance using transient genetic tools (siRNA, cDNA transfection) (28, 67).
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PMC11204465
One new compound, 11-hydroxy-O-methylsterigmatocystin (53), was isolated from Aspergillus sp. .
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PMC8164677
All stocks were prepared in 5 mL tubes.
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