Datasets:
OTAR3088
/
BioData-CellLine_annotated

Dataset card Data Studio Files
xet
Community
1
PMCID
string
Sentences
string
ner
list
PMC2193049
The production of the latter appears to be inhibited by lactacystin.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "The", "production", "of", "the", "latter", "appears", "to", "be", "inhibited", "by", "lactacystin", "." ] } ]
PMC11742231
Generalization to New Cell Lines: In the new cell line test experiment, MGATAF outperforms all baseline models, demonstrating its robustness and generalization capability to unseen cell lines.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Generalization", "to", "New", "Cell", "Lines", ":", "In", "the", "new", "cell", "line", "test", "experiment", ",", "MGATAF", "outperforms", "all", "baseline", "models", ",", "demonstrating", "its", "robustness", "and", "generalization", "capability", "to", "unseen", "cell", "lines", "." ] } ]
PMC11461025
The membranes were incubated for 1 hour at room temperature with HRP-conjugated anti-rabbit and anti-mouse antibodies used at 1:5000.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "The", "membranes", "were", "incubated", "for", "1", "hour", "at", "room", "temperature", "with", "HRP-conjugated", "anti-rabbit", "and", "anti-mouse", "antibodies", "used", "at", "1:5000", "." ] } ]
PMC11790599
This unusual form of cell death has been observed in certain cancer cells treated with certain small molecules.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "This", "unusual", "form", "of", "cell", "death", "has", "been", "observed", "in", "certain", "cancer", "cells", "treated", "with", "certain", "small", "molecules", "." ] } ]
PMC8348645
The dried plant samples were crushed and powdered manually and with the use of a mixer for a few seconds to turn the seeds into powder.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "The", "dried", "plant", "samples", "were", "crushed", "and", "powdered", "manually", "and", "with", "the", "use", "of", "a", "mixer", "for", "a", "few", "seconds", "to", "turn", "the", "seeds", "into", "powder", "." ] } ]
PMC5034105
Moreover, recent clinico-epidemiological, laboratory and animal studies on pathogenesis have established the association between ZIKV infection and congenital anomalies, such as microcephaly, central nervous system malformations and ophthalmological abnormalities.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Moreover", ",", "recent", "clinico-epidemiological", ",", "laboratory", "and", "animal", "studies", "on", "pathogenesis", "have", "established", "the", "association", "between", "ZIKV", "infection", "and", "congenital", "anomalies", ",", "such", "as", "microcephaly", ",", "central", "nervous", "system", "malformations", "and", "ophthalmological", "abnormalities", "." ] } ]
PMC11513011
Western blotting results showed that DIB treatment decreased FoxM1 expression in KK-47, T24 and 5637 cells.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "B-CellLine", "O", "B-CellLine", "O", "B-CellLine", "O", "O" ], "tokens": [ "Western", "blotting", "results", "showed", "that", "DIB", "treatment", "decreased", "FoxM1", "expression", "in", "KK-47", ",", "T24", "and", "5637", "cells", "." ] } ]
PMC11240571
Results: Exosomal PD-L1 was detected in the culture medium of tumor cells but was absent in the culture medium of PD-L1 knockout cells.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Results", ":", "Exosomal", "PD-L1", "was", "detected", "in", "the", "culture", "medium", "of", "tumor", "cells", "but", "was", "absent", "in", "the", "culture", "medium", "of", "PD-L1", "knockout", "cells", "." ] } ]
PMC11469524
Upstream kinase activity assessment on base of log2 fold changes (vs. α-IgM+DMSO) in PhosphoSitePlus-annotated target sites (Nov 2021) for selected kinases.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Upstream", "kinase", "activity", "assessment", "on", "base", "of", "log2", "fold", "changes", "(", "vs.", "α-IgM+DMSO", ")", "in", "PhosphoSitePlus-annotated", "target", "sites", "(", "Nov", "2021", ")", "for", "selected", "kinases", "." ] } ]
PMC9429973
The median OS from time of HSCT was 13.4 months, and the 1-year post-HSCT OS was 59%.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "The", "median", "OS", "from", "time", "of", "HSCT", "was", "13.4", "months", ",", "and", "the", "1-year", "post-HSCT", "OS", "was", "59", "%", "." ] } ]
PMC10898159
Transcription factor MITF and BIRC5 promote KIT transcription.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Transcription", "factor", "MITF", "and", "BIRC5", "promote", "KIT", "transcription", "." ] } ]
PMC9429973
and observed the complete response rate (CR), total response rate (ORR) and the occurrence of adverse reactions in 21 patients,after 1 course of induction chemotherapy.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "and", "observed", "the", "complete", "response", "rate", "(", "CR", ")", ",", "total", "response", "rate", "(", "ORR", ")", "and", "the", "occurrence", "of", "adverse", "reactions", "in", "21", "patients", ",", "after", "1", "course", "of", "induction", "chemotherapy", "." ] } ]
PMC10998613
FKBP oligomer controls were based on the FK506-binding protein, as previously described (31), The constructs were transiently expressed in COS-7 cells after which the dimerizing agent (AP20187) was added, and after 20 min, PIE-FCCS were collected.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "FKBP", "oligomer", "controls", "were", "based", "on", "the", "FK506-binding", "protein", ",", "as", "previously", "described", "(", "31", ")", ",", "The", "constructs", "were", "transiently", "expressed", "in", "COS-7", "cells", "after", "which", "the", "dimerizing", "agent", "(", "AP20187", ")", "was", "added", ",", "and", "after", "20", "min", ",", "PIE-FCCS", "were", "collected", "." ] } ]
PMC9516447
Detailed procedures for RNA extraction, reverse transcription and quantitative real-time PCR have been previously described .
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Detailed", "procedures", "for", "RNA", "extraction", ",", "reverse", "transcription", "and", "quantitative", "real-time", "PCR", "have", "been", "previously", "described", "." ] } ]
PMC11632064
Research hypothesis.
[ { "tags": [ "O", "O", "O" ], "tokens": [ "Research", "hypothesis", "." ] } ]
PMC11291490
This outcome might be caused by two potential factors.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "This", "outcome", "might", "be", "caused", "by", "two", "potential", "factors", "." ] } ]
PMC10994876
The outcomes presented in Fig. 8 exemplify that the YAP5SA overexpressing cells produced approximately three times more total protein than the EPO-CHO cells transfected with the blank construct and NT cells (P-value < 0.0001).
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "B-CellLine", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "The", "outcomes", "presented", "in", "Fig.", "8", "exemplify", "that", "the", "YAP5SA", "overexpressing", "cells", "produced", "approximately", "three", "times", "more", "total", "protein", "than", "the", "EPO-CHO", "cells", "transfected", "with", "the", "blank", "construct", "and", "NT", "cells", "(", "P-value", "<", "0.0001", ")", "." ] } ]
PMC9429973
Ponatinib (PON) is a potent third-generation TKI active against BCR::ABL1 and all identified single resistance mutations, including the gatekeeper T315I mutation that confers resistance to other TKIs.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Ponatinib", "(", "PON", ")", "is", "a", "potent", "third-generation", "TKI", "active", "against", "BCR::ABL1", "and", "all", "identified", "single", "resistance", "mutations", ",", "including", "the", "gatekeeper", "T315I", "mutation", "that", "confers", "resistance", "to", "other", "TKIs", "." ] } ]
PMC11532793
As a result, bootstrap sampling will fail to accurately reproduce the variability associated with resequencing.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "As", "a", "result", ",", "bootstrap", "sampling", "will", "fail", "to", "accurately", "reproduce", "the", "variability", "associated", "with", "resequencing", "." ] } ]
PMC10747139
The octahedral polypyridyl Ru(II) complex TLD1433 has potential as a photosensitizer for photodynamic therapy in the treatment of bladder cancer .
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "The", "octahedral", "polypyridyl", "Ru(II", ")", "complex", "TLD1433", "has", "potential", "as", "a", "photosensitizer", "for", "photodynamic", "therapy", "in", "the", "treatment", "of", "bladder", "cancer", "." ] } ]
PMC6267596
On the other hand, all left most (same vertical line) genes were identified only in the DMSO control but absent in 8n-cells.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "On", "the", "other", "hand", ",", "all", "left", "most", "(", "same", "vertical", "line", ")", "genes", "were", "identified", "only", "in", "the", "DMSO", "control", "but", "absent", "in", "8n-cells", "." ] } ]
PMC3765139
Based on these results, gene expression profiling using gene chips was performed on CD133+ and CD133- D10 cells.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Based", "on", "these", "results", ",", "gene", "expression", "profiling", "using", "gene", "chips", "was", "performed", "on", "CD133", "+", "and", "CD133-", "D10", "cells", "." ] } ]
PMC11469524
More detailed modeling workflows as well as the required data for all modelled cell lines and scenarios are provided by step-by-step accompanying html reports available online (S1 Text, https://zenodo.org/doi/10.5281/zenodo.10732059).
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "More", "detailed", "modeling", "workflows", "as", "well", "as", "the", "required", "data", "for", "all", "modelled", "cell", "lines", "and", "scenarios", "are", "provided", "by", "step-by-step", "accompanying", "html", "reports", "available", "online", "(", "S1", "Text", ",", "https://zenodo.org/doi/10.5281/zenodo.10732059", ")", "." ] } ]
PMC10936243
Especially in this cat you see here, we put in towels, we put in heater as well.” [
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Especially", "in", "this", "cat", "you", "see", "here", ",", "we", "put", "in", "towels", ",", "we", "put", "in", "heater", "as", "well", ".", "”", "[" ] } ]
PMC9920575
After 30 min in the dark, we washed both samples (the samples stained with caspase-3 PE and p53 Alexa Fluor647) before analyzing them using a BD FACSAria II flow cytometer (Biosciences, NJ, USA).
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "After", "30", "min", "in", "the", "dark", ",", "we", "washed", "both", "samples", "(", "the", "samples", "stained", "with", "caspase-3", "PE", "and", "p53", "Alexa", "Fluor647", ")", "before", "analyzing", "them", "using", "a", "BD", "FACSAria", "II", "flow", "cytometer", "(", "Biosciences", ",", "NJ", ",", "USA", ")", "." ] } ]
PMC10218459
Therefore, it is conceivable that the CPP treatment of Ewing’s sarcoma in clinical practice could be performed for the first time and once intraoperatively, with surgical removal of the tumor.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Therefore", ",", "it", "is", "conceivable", "that", "the", "CPP", "treatment", "of", "Ewing", "’s", "sarcoma", "in", "clinical", "practice", "could", "be", "performed", "for", "the", "first", "time", "and", "once", "intraoperatively", ",", "with", "surgical", "removal", "of", "the", "tumor", "." ] } ]
PMC11453018
Gene expression quantification was anchored to GAPDH or B2M reference genes and computed using the 7500 System Software through the ΔCT method by Applied Biosystems.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Gene", "expression", "quantification", "was", "anchored", "to", "GAPDH", "or", "B2", "M", "reference", "genes", "and", "computed", "using", "the", "7500", "System", "Software", "through", "the", "ΔCT", "method", "by", "Applied", "Biosystems", "." ] } ]
PMC11486946
Slit images, which reflect deviation from the in-focus position of the objective lens, are generated with switching illumination and used for feedback control.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Slit", "images", ",", "which", "reflect", "deviation", "from", "the", "in-focus", "position", "of", "the", "objective", "lens", ",", "are", "generated", "with", "switching", "illumination", "and", "used", "for", "feedback", "control", "." ] } ]
PMC10006224
After 72 h, cells were seeded at low density for clonal selection.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "After", "72", "h", ",", "cells", "were", "seeded", "at", "low", "density", "for", "clonal", "selection", "." ] } ]
PMC11269933
The CgPK genes are marked with red dotted lines.(B) Conservative analysis of PK sequences in metazoan.(C) The sequence alignment surrounding S11 on CgPK.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "The", "CgPK", "genes", "are", "marked", "with", "red", "dotted", "lines.(B", ")", "Conservative", "analysis", "of", "PK", "sequences", "in", "metazoan.(C", ")", "The", "sequence", "alignment", "surrounding", "S11", "on", "CgPK", "." ] } ]
PMC7433824
M1 isoform is mainly found in muscle, heart, and brain, while M2 exists in early fetal tissues and most cancer cells.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "M1", "isoform", "is", "mainly", "found", "in", "muscle", ",", "heart", ",", "and", "brain", ",", "while", "M2", "exists", "in", "early", "fetal", "tissues", "and", "most", "cancer", "cells", "." ] } ]
PMC9031474
It was also shown that cancer cell exposure to drimane-containing plant extracts can cause cell cycle arrest.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "It", "was", "also", "shown", "that", "cancer", "cell", "exposure", "to", "drimane-containing", "plant", "extracts", "can", "cause", "cell", "cycle", "arrest", "." ] } ]
PMC10440586
The P value was calculated by T-test. (
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "The", "P", "value", "was", "calculated", "by", "T-test", ".", "(" ] } ]
PMC11770130
Previously, we have reported that NTS controlled the MEK-ERK signaling in BATs.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Previously", ",", "we", "have", "reported", "that", "NTS", "controlled", "the", "MEK-ERK", "signaling", "in", "BATs", "." ] } ]
PMC4816271
To achieve this, we co-expressed the inducible Caspase 9 (iCasp9) suicide gene .
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "To", "achieve", "this", ",", "we", "co-expressed", "the", "inducible", "Caspase", "9", "(", "iCasp9", ")", "suicide", "gene", "." ] } ]
PMC7709199
Protein yields from a 2.4 l GD culture were typically 0.7 mg l after nickel-affinity purification, size-exclusion chromatography (SEC) and sample concentration, whereas the wild-type cells yielded 1.25 mg l. This reduction in protein yield from the engineered cell line is similar to that observed for HEK293S MGAT1 cells.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "B-CellLine", "B-CellLine", "O", "O" ], "tokens": [ "Protein", "yields", "from", "a", "2.4", "l", "GD", "culture", "were", "typically", "0.7", "mg", "l", "after", "nickel-affinity", "purification", ",", "size-exclusion", "chromatography", "(", "SEC", ")", "and", "sample", "concentration", ",", "whereas", "the", "wild-type", "cells", "yielded", "1.25", "mg", "l.", "This", "reduction", "in", "protein", "yield", "from", "the", "engineered", "cell", "line", "is", "similar", "to", "that", "observed", "for", "HEK293S", "MGAT1", "cells", "." ] } ]
PMC4816271
PBMCs were transduced as described with equal titres of the indicated CAR retroviral supernatant.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "PBMCs", "were", "transduced", "as", "described", "with", "equal", "titres", "of", "the", "indicated", "CAR", "retroviral", "supernatant", "." ] } ]
PMC7081114
A and B: Low expression of KIF21B in normal adjacent tissues (magnification: ×100 for A and ×400 for B); C and D: High expression of KIF21B in hepatocellular carcinoma tissues (magnification: ×100 for C and ×400 for D); E and F: Kaplan-Meier’s survival curves using a log-rank test demonstrating the correlation between KIF21B expression and overall survival or disease-free survival.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "A", "and", "B", ":", "Low", "expression", "of", "KIF21B", "in", "normal", "adjacent", "tissues", "(", "magnification", ":", "×100", "for", "A", "and", "×400", "for", "B", ")", ";", "C", "and", "D", ":", "High", "expression", "of", "KIF21B", "in", "hepatocellular", "carcinoma", "tissues", "(", "magnification", ":", "×100", "for", "C", "and", "×400", "for", "D", ")", ";", "E", "and", "F", ":", "Kaplan-Meier", "’s", "survival", "curves", "using", "a", "log-rank", "test", "demonstrating", "the", "correlation", "between", "KIF21B", "expression", "and", "overall", "survival", "or", "disease-free", "survival", "." ] } ]
PMC10854447
Caspase-3, caspase-8, Bax, and Bcl-2 levels of compounds 7j, 7k, and 7l.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Caspase-3", ",", "caspase-8", ",", "Bax", ",", "and", "Bcl-2", "levels", "of", "compounds", "7j", ",", "7k", ",", "and", "7l", "." ] } ]
PMC11585254
The adjusted P values for all comparisons were greater than 0.05.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "The", "adjusted", "P", "values", "for", "all", "comparisons", "were", "greater", "than", "0.05", "." ] } ]
PMC11261875
GLUT-2 is predominantly localized in pancreatic beta cells, hepatocytes, and renal tubular cells, whereas GLUT-3 is mainly expressed in nervous tissues .
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "GLUT-2", "is", "predominantly", "localized", "in", "pancreatic", "beta", "cells", ",", "hepatocytes", ",", "and", "renal", "tubular", "cells", ",", "whereas", "GLUT-3", "is", "mainly", "expressed", "in", "nervous", "tissues", "." ] } ]
PMC10976516
Figure 5Advantage of systemic delivery of an MSC-shielded OVSystemically injected unprotected OVs, such as the virus, cause an antiviral response through innate (NK cells, cytokines, mononuclear phagocyte system (MPS), complement activation) and eventual adaptive (antibodies, T cell mediated) immunity, which clears the virus and prevents any oncolytic effects.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Figure", "5Advantage", "of", "systemic", "delivery", "of", "an", "MSC-shielded", "OVSystemically", "injected", "unprotected", "OVs", ",", "such", "as", "the", "virus", ",", "cause", "an", "antiviral", "response", "through", "innate", "(", "NK", "cells", ",", "cytokines", ",", "mononuclear", "phagocyte", "system", "(", "MPS", ")", ",", "complement", "activation", ")", "and", "eventual", "adaptive", "(", "antibodies", ",", "T", "cell", "mediated", ")", "immunity", ",", "which", "clears", "the", "virus", "and", "prevents", "any", "oncolytic", "effects", "." ] } ]
PMC10443665
The main ingredients of A. membranaceus are Astragalus polysaccharides (APS).
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "The", "main", "ingredients", "of", "A.", "membranaceus", "are", "Astragalus", "polysaccharides", "(", "APS", ")", "." ] } ]
PMC9429973
D5PSS-1-3:Only one relapse was reported among 71 D5PSS-1-3 patients during a median follow-up of 24.2 months (0.5-85).
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "D5PSS-1", "-", "3:Only", "one", "relapse", "was", "reported", "among", "71", "D5PSS-1", "-", "3", "patients", "during", "a", "median", "follow-up", "of", "24.2", "months", "(", "0.5", "-", "85", ")", "." ] } ]
PMC11741577
They are implicated in epithelialization through which they restore the epidermal barrier by migrating, proliferating, and differentiating .
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "They", "are", "implicated", "in", "epithelialization", "through", "which", "they", "restore", "the", "epidermal", "barrier", "by", "migrating", ",", "proliferating", ",", "and", "differentiating", "." ] } ]
PMC11351758
Accordingly, mitochondrial calcium [Ca]m overload leads to ROS production, which can be lethal to the mitochondria through various mechanisms.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Accordingly", ",", "mitochondrial", "calcium", "[Ca]m", "overload", "leads", "to", "ROS", "production", ",", "which", "can", "be", "lethal", "to", "the", "mitochondria", "through", "various", "mechanisms", "." ] } ]
PMC8540692
The permeability through the blood-brain barrier (BBB) and through the nasal cavity was evaluated using PAMPA model.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "The", "permeability", "through", "the", "blood-brain", "barrier", "(", "BBB", ")", "and", "through", "the", "nasal", "cavity", "was", "evaluated", "using", "PAMPA", "model", "." ] } ]
PMC6163708
Results of both experiments were similar.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Results", "of", "both", "experiments", "were", "similar", "." ] } ]
PMC11534377
Microarray analysis revealed significant changes in gene expression, including the upregulation of apoptosis-related genes and the downregulation of cell proliferation-related genes.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Microarray", "analysis", "revealed", "significant", "changes", "in", "gene", "expression", ",", "including", "the", "upregulation", "of", "apoptosis-related", "genes", "and", "the", "downregulation", "of", "cell", "proliferation-related", "genes", "." ] } ]
PMC8913139
miR-448 restrained cell viability, invasion, and migration in GBM by inhibiting ROCK1 expression.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "miR-448", "restrained", "cell", "viability", ",", "invasion", ",", "and", "migration", "in", "GBM", "by", "inhibiting", "ROCK1", "expression", "." ] } ]
PMC11180402
Genomic DNA was isolated from 5637 cells using the DNeasy Blood & Tissue Kit (Qiagen, Gaithersburg, MD, USA), following the kit's specific protocols.
[ { "tags": [ "O", "O", "O", "O", "O", "B-CellLine", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Genomic", "DNA", "was", "isolated", "from", "5637", "cells", "using", "the", "DNeasy", "Blood", "&", "Tissue", "Kit", "(", "Qiagen", ",", "Gaithersburg", ",", "MD", ",", "USA", ")", ",", "following", "the", "kit", "'s", "specific", "protocols", "." ] } ]
PMC9917080
Following 48 h of So exposure to A-172 glioblastoma cells, there was no significant change in the expression of genes encoding selenoproteins, and the level of genes encoding selenium-containing proteins GPX1, GPX4, TR1, and TR3 significantly increased (Figure 9B,F).
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "B-CellLine", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Following", "48", "h", "of", "So", "exposure", "to", "A-172", "glioblastoma", "cells", ",", "there", "was", "no", "significant", "change", "in", "the", "expression", "of", "genes", "encoding", "selenoproteins", ",", "and", "the", "level", "of", "genes", "encoding", "selenium-containing", "proteins", "GPX1", ",", "GPX4", ",", "TR1", ",", "and", "TR3", "significantly", "increased", "(", "Figure", "9B", ",", "F", ")", "." ] } ]
PMC9388315
Data analysis was performed by IBM SPSS Statistics 16.0 (IBM, USA) and Microsoft Graph Pad Prism 6 (Graph Pad Prism Inc., USA) used for drawing graphs.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Data", "analysis", "was", "performed", "by", "IBM", "SPSS", "Statistics", "16.0", "(", "IBM", ",", "USA", ")", "and", "Microsoft", "Graph", "Pad", "Prism", "6", "(", "Graph", "Pad", "Prism", "Inc.", ",", "USA", ")", "used", "for", "drawing", "graphs", "." ] } ]
PMC11285179
Statistical analyses were performed on GraphPad Prism 8.0.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Statistical", "analyses", "were", "performed", "on", "GraphPad", "Prism", "8.0", "." ] } ]
PMC9429973
During hospital admission due to chronic kidney disease exacerbation he developed an Atrial Fibrillation and initiated treatment with low molecular weight heparin (LMWH).
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "During", "hospital", "admission", "due", "to", "chronic", "kidney", "disease", "exacerbation", "he", "developed", "an", "Atrial", "Fibrillation", "and", "initiated", "treatment", "with", "low", "molecular", "weight", "heparin", "(", "LMWH", ")", "." ] } ]
PMC9100622
The sensitivity of Ewing cells to statins is in full agreement with data from large CRSPR/Cas9-based and pharmacological dependency screenings .
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "The", "sensitivity", "of", "Ewing", "cells", "to", "statins", "is", "in", "full", "agreement", "with", "data", "from", "large", "CRSPR/Cas9-based", "and", "pharmacological", "dependency", "screenings", "." ] } ]
PMC11306019
Evaluations of QC, CC, and EC, all possessing a C3-OH group, revealed weak antagonistic effects for CC and EC due to their non-aromatic C-ring.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Evaluations", "of", "QC", ",", "CC", ",", "and", "EC", ",", "all", "possessing", "a", "C3-OH", "group", ",", "revealed", "weak", "antagonistic", "effects", "for", "CC", "and", "EC", "due", "to", "their", "non-aromatic", "C-ring", "." ] } ]
PMC11756514
MBL binding was significantly increased for the JE2ΔtarM mutant compared to the WT strain, whereas there was an apparent loss of binding to the JE2ΔtarMS and JE2ΔtagO mutants ( Figure 4B ).
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "MBL", "binding", "was", "significantly", "increased", "for", "the", "JE2ΔtarM", "mutant", "compared", "to", "the", "WT", "strain", ",", "whereas", "there", "was", "an", "apparent", "loss", "of", "binding", "to", "the", "JE2ΔtarMS", "and", "JE2ΔtagO", "mutants", "(", "Figure", "4B", ")", "." ] } ]
PMC7433824
In our analyses, CH60/HSPD1/HSP60 also emerged as a component of the pathway enrichment analysis regarding TFAP2A mediates retinoic acid‐induced cellular differentiation via acting as a transcriptional repressor.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "In", "our", "analyses", ",", "CH60/HSPD1/HSP60", "also", "emerged", "as", "a", "component", "of", "the", "pathway", "enrichment", "analysis", "regarding", "TFAP2A", "mediates", "retinoic", "acid‐induced", "cellular", "differentiation", "via", "acting", "as", "a", "transcriptional", "repressor", "." ] } ]
PMC11541241
tomato, and the AtFKBP12‐knockout mutant enhanced the plant's ability to defend against this pathogen.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "tomato", ",", "and", "the", "AtFKBP12‐knockout", "mutant", "enhanced", "the", "plant", "'s", "ability", "to", "defend", "against", "this", "pathogen", "." ] } ]
PMC11473750
qRT-PCR for gene expression was analysed using the Quantstudio™ 3 real-time PCR machine (ThermoFisher Scientific, Waltham, MA, USA).
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "qRT-PCR", "for", "gene", "expression", "was", "analysed", "using", "the", "Quantstudio", "™", "3", "real-time", "PCR", "machine", "(", "ThermoFisher", "Scientific", ",", "Waltham", ",", "MA", ",", "USA", ")", "." ] } ]
PMC5034105
Incomplete Freund's adjuvant (Sigma-Aldrich) was used in subsequent injections at 14-day intervals for three times.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Incomplete", "Freund", "'s", "adjuvant", "(", "Sigma-Aldrich", ")", "was", "used", "in", "subsequent", "injections", "at", "14-day", "intervals", "for", "three", "times", "." ] } ]
PMC9429973
Aims: The objective of our study was to determine the post-splenectomy incidence of invasive bacterial infections and thrombo-embolic complications in a large cohort of children with SCA.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Aims", ":", "The", "objective", "of", "our", "study", "was", "to", "determine", "the", "post-splenectomy", "incidence", "of", "invasive", "bacterial", "infections", "and", "thrombo-embolic", "complications", "in", "a", "large", "cohort", "of", "children", "with", "SCA", "." ] } ]
PMC9351137
Scale bar = 5 μm.
[ { "tags": [ "O", "O", "O", "O", "O", "O" ], "tokens": [ "Scale", "bar", "=", "5", "μm", "." ] } ]
PMC11621565
LPS concentrations induced a discrete dose-dependent increase of mRNA mean levels but without statistical significance.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "LPS", "concentrations", "induced", "a", "discrete", "dose-dependent", "increase", "of", "mRNA", "mean", "levels", "but", "without", "statistical", "significance", "." ] } ]
PMC11049548
Matching between new NMR data and databases was performed using the AMIX 4.0.2 software (Bruker BioSpin, Rheinstetten, Germany).
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Matching", "between", "new", "NMR", "data", "and", "databases", "was", "performed", "using", "the", "AMIX", "4.0.2", "software", "(", "Bruker", "BioSpin", ",", "Rheinstetten", ",", "Germany", ")", "." ] } ]
PMC10565698
L. miR-211-5p correlation analysis with TCF12.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "L.", "miR-211", "-", "5p", "correlation", "analysis", "with", "TCF12", "." ] } ]
PMC9429973
Estimated half-life was between 18 and 55 hours.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Estimated", "half-life", "was", "between", "18", "and", "55", "hours", "." ] } ]
PMC11806818
Therefore, this section will comprehensively summarize the relevant research advances of highly sensitive and specific nanozyme-based single-mode aptamer biosensors for the detection of protein expression on exosome surfaces, discusses the potential of their application for the detection of real human blood samples, and compares the assay performance of individual sensors in Table 2.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Therefore", ",", "this", "section", "will", "comprehensively", "summarize", "the", "relevant", "research", "advances", "of", "highly", "sensitive", "and", "specific", "nanozyme-based", "single-mode", "aptamer", "biosensors", "for", "the", "detection", "of", "protein", "expression", "on", "exosome", "surfaces", ",", "discusses", "the", "potential", "of", "their", "application", "for", "the", "detection", "of", "real", "human", "blood", "samples", ",", "and", "compares", "the", "assay", "performance", "of", "individual", "sensors", "in", "Table", "2", "." ] } ]
PMC11394227
During the initial five days of the culture period, the cell viability of CHO-PAb and CHO-PAb-QS decreased slowly but remained above 90% (Figure 2A).
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "During", "the", "initial", "five", "days", "of", "the", "culture", "period", ",", "the", "cell", "viability", "of", "CHO-PAb", "and", "CHO-PAb-QS", "decreased", "slowly", "but", "remained", "above", "90", "%", "(", "Figure", "2A", ")", "." ] } ]
PMC7762347
Three independent experiments were done, for each treatment time in five replicates per treatment point.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Three", "independent", "experiments", "were", "done", ",", "for", "each", "treatment", "time", "in", "five", "replicates", "per", "treatment", "point", "." ] } ]
PMC11577421
They were characterized by DLS and ZP measurements in phosphate buffer (10 mM, pH 7.4) employing a Zetasizer Nano ZS instrument (Malvern Instruments, UK).
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "They", "were", "characterized", "by", "DLS", "and", "ZP", "measurements", "in", "phosphate", "buffer", "(", "10", "mM", ",", "pH", "7.4", ")", "employing", "a", "Zetasizer", "Nano", "ZS", "instrument", "(", "Malvern", "Instruments", ",", "UK", ")", "." ] } ]
PMC9318744
Taken together, these results provide evidence that the ACE2plusC3 cell line is a suitable model for SARS-CoV-2 research.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "B-CellLine", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Taken", "together", ",", "these", "results", "provide", "evidence", "that", "the", "ACE2plusC3", "cell", "line", "is", "a", "suitable", "model", "for", "SARS-CoV-2", "research", "." ] } ]
PMC11506827
The quadrants were divided into Q3-1 (Annexin V−/PI+, necrotic cells), Q3-2 (Annexin V+/PI+, late apoptotic cells), Q3-3 (Annexin V−/PI−, healthy cells), and Q3-4 (Annexin V+/PI−, early apoptotic cells), respectively.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "The", "quadrants", "were", "divided", "into", "Q3", "-", "1", "(", "Annexin", "V−/PI+", ",", "necrotic", "cells", ")", ",", "Q3", "-", "2", "(", "Annexin", "V+/PI+", ",", "late", "apoptotic", "cells", ")", ",", "Q3", "-", "3", "(", "Annexin", "V−/PI−", ",", "healthy", "cells", ")", ",", "and", "Q3", "-", "4", "(", "Annexin", "V+/PI−", ",", "early", "apoptotic", "cells", ")", ",", "respectively", "." ] } ]
PMC9727330
Typically, asparagine synthetase (ASNS) is an enzyme found in all mammalian organs required to convert glutamine to glutamate and aspartate to Asp-dependent ATP molecules (Lomelino et al., 2017).
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Typically", ",", "asparagine", "synthetase", "(", "ASNS", ")", "is", "an", "enzyme", "found", "in", "all", "mammalian", "organs", "required", "to", "convert", "glutamine", "to", "glutamate", "and", "aspartate", "to", "Asp-dependent", "ATP", "molecules", "(", "Lomelino", "et", "al.", ",", "2017", ")", "." ] } ]
PMC11787355
Subsequently, concentration of solvent B was increased to 80% in 2.6 min and kept at 80% solvent B for 5 min for washing.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Subsequently", ",", "concentration", "of", "solvent", "B", "was", "increased", "to", "80", "%", "in", "2.6", "min", "and", "kept", "at", "80", "%", "solvent", "B", "for", "5", "min", "for", "washing", "." ] } ]
PMC10728200
Significance denoted by: ns not significant, *P < 0.05, **P < 0.01, and ***P < 0.001.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Significance", "denoted", "by", ":", "ns", "not", "significant", ",", "*", "P", "<", "0.05", ",", "*", "*", "P", "<", "0.01", ",", "and", "*", "*", "*", "P", "<", "0.001", "." ] } ]
PMC11478724
The temperature protocol used for amplification was a cycle at 95°C for 15 min, followed by 40 cycles of 15 s at 95°C, 15 s at the annealing temperature, and 10 s at 72°C.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "The", "temperature", "protocol", "used", "for", "amplification", "was", "a", "cycle", "at", "95", "°", "C", "for", "15", "min", ",", "followed", "by", "40", "cycles", "of", "15", "s", "at", "95", "°", "C", ",", "15", "s", "at", "the", "annealing", "temperature", ",", "and", "10", "s", "at", "72", "°", "C", "." ] } ]
PMC11055510
Such as circ-ITCH, it acts as a ceRNA sponge for miR-145, which can increase the protein level of RASA1 and inhibit the malignant progression of OC cells in vitro and in vivo through the circ-ITCH–miR-145–RASA1 axis, contributing to the occurrence and development of cancer (Hu et al., 2018).
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Such", "as", "circ-ITCH", ",", "it", "acts", "as", "a", "ceRNA", "sponge", "for", "miR-145", ",", "which", "can", "increase", "the", "protein", "level", "of", "RASA1", "and", "inhibit", "the", "malignant", "progression", "of", "OC", "cells", "in", "vitro", "and", "in", "vivo", "through", "the", "circ-ITCH", "–", "miR-145–RASA1", "axis", ",", "contributing", "to", "the", "occurrence", "and", "development", "of", "cancer", "(", "Hu", "et", "al.", ",", "2018", ")", "." ] } ]
PMC10870498
However, there are still some limitations in this study, for example, the results of this study only represent experiments in vitro, and the results in vivo will be further analyzed in future studies.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "However", ",", "there", "are", "still", "some", "limitations", "in", "this", "study", ",", "for", "example", ",", "the", "results", "of", "this", "study", "only", "represent", "experiments", "in", "vitro", ",", "and", "the", "results", "in", "vivo", "will", "be", "further", "analyzed", "in", "future", "studies", "." ] } ]
PMC11803149
The right panel presents a stacked bar graph showing percentages of cells in each t-SNE cluster. (
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "The", "right", "panel", "presents", "a", "stacked", "bar", "graph", "showing", "percentages", "of", "cells", "in", "each", "t-SNE", "cluster", ".", "(" ] } ]
PMC11705862
Knockdown of CD59 was performed and its efficiency verified in the same way as described in the “Noradrenaline secretion assay” methods section.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Knockdown", "of", "CD59", "was", "performed", "and", "its", "efficiency", "verified", "in", "the", "same", "way", "as", "described", "in", "the", "“", "Noradrenaline", "secretion", "assay", "”", "methods", "section", "." ] } ]
PMC11012313
That being said, previous studies using cell lines that were exposed to hypoxic conditions induced a switch from latency to lytic replication and required the involvement of HIF-1α .
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "That", "being", "said", ",", "previous", "studies", "using", "cell", "lines", "that", "were", "exposed", "to", "hypoxic", "conditions", "induced", "a", "switch", "from", "latency", "to", "lytic", "replication", "and", "required", "the", "involvement", "of", "HIF-1α", "." ] } ]
PMC11348300
Time transients were derived by averaging pixel intensities within ROIs across both normal and tumor tissue regions.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Time", "transients", "were", "derived", "by", "averaging", "pixel", "intensities", "within", "ROIs", "across", "both", "normal", "and", "tumor", "tissue", "regions", "." ] } ]
PMC11705630
Therefore, we hypothesized that knockdown of GLDC would attenuate de novo nucleotide synthesis eventually decreasing intracellular dNTP pools.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Therefore", ",", "we", "hypothesized", "that", "knockdown", "of", "GLDC", "would", "attenuate", "de", "novo", "nucleotide", "synthesis", "eventually", "decreasing", "intracellular", "dNTP", "pools", "." ] } ]
PMC9429973
Moreover, the expression of cytochrome C (Cyt-C), Apaf-1, Caspase-9 and Caspase-3 at mRNA and protein levels was further determined by using quantitative PCR (Q-PCR) and western blotting.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Moreover", ",", "the", "expression", "of", "cytochrome", "C", "(", "Cyt-C", ")", ",", "Apaf-1", ",", "Caspase-9", "and", "Caspase-3", "at", "mRNA", "and", "protein", "levels", "was", "further", "determined", "by", "using", "quantitative", "PCR", "(", "Q-PCR", ")", "and", "western", "blotting", "." ] } ]
PMC7351993
Processing of the raw images was performed using ImageJ. Scale bars, 10 µm.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Processing", "of", "the", "raw", "images", "was", "performed", "using", "ImageJ.", "Scale", "bars", ",", "10", "µm", "." ] } ]
PMC11787355
i, j Cellular states of cancer cells in c and PTPRZ1 expression.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "i", ",", "j", "Cellular", "states", "of", "cancer", "cells", "in", "c", "and", "PTPRZ1", "expression", "." ] } ]
PMC11658074
a Schematic diagram of sample collection and detection.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "a", "Schematic", "diagram", "of", "sample", "collection", "and", "detection", "." ] } ]
PMC11612626
Our MS/proteomic studies indicate that compound A treatment of multiple myeloma cells increased the presentation of numerous peptide epitopes as a consequence of increased processing of the same protein.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Our", "MS/proteomic", "studies", "indicate", "that", "compound", "A", "treatment", "of", "multiple", "myeloma", "cells", "increased", "the", "presentation", "of", "numerous", "peptide", "epitopes", "as", "a", "consequence", "of", "increased", "processing", "of", "the", "same", "protein", "." ] } ]
PMC11687167
Previous reports have shown that cholesterol on cell membranes functions in the recycling of major histocompatibility complex class I (MHC-I) molecules .
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Previous", "reports", "have", "shown", "that", "cholesterol", "on", "cell", "membranes", "functions", "in", "the", "recycling", "of", "major", "histocompatibility", "complex", "class", "I", "(", "MHC-I", ")", "molecules", "." ] } ]
PMC11206251
Notably, the expression of p53 mRNA and caspase-3 mRNA showed a significant difference (p < 0.05) compared to the control group after treatment with different concentrations of 50 μg·mL PEIM.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Notably", ",", "the", "expression", "of", "p53", "mRNA", "and", "caspase-3", "mRNA", "showed", "a", "significant", "difference", "(", "p", "<", "0.05", ")", "compared", "to", "the", "control", "group", "after", "treatment", "with", "different", "concentrations", "of", "50", "μg·mL", "PEIM", "." ] } ]
PMC11792090
In terms of anti-tumor efficacy, during the sustained tumor-killing period, SAP UCAR-T cells consistently displayed strong tumor-killing capabilities, comparable to those of mock CAR-T cells ( Figure 5D ).
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "In", "terms", "of", "anti-tumor", "efficacy", ",", "during", "the", "sustained", "tumor-killing", "period", ",", "SAP", "UCAR-T", "cells", "consistently", "displayed", "strong", "tumor-killing", "capabilities", ",", "comparable", "to", "those", "of", "mock", "CAR-T", "cells", "(", "Figure", "5D", ")", "." ] } ]
PMC11535726
Hernández-Restrepo et al. (2017) established a novel order, Kirschsteiniotheliales, to accommodate the Kirschsteiniotheliaceae taxa.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Hernández-Restrepo", "et", "al.", "(", "2017", ")", "established", "a", "novel", "order", ",", "Kirschsteiniotheliales", ",", "to", "accommodate", "the", "Kirschsteiniotheliaceae", "taxa", "." ] } ]
PMC11765243
Furthermore, we need to know the effect on immune parameters of PI3K inhibitors with broad or narrow specificity, of interest in cancer, metabolism, or autoimmunity.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Furthermore", ",", "we", "need", "to", "know", "the", "effect", "on", "immune", "parameters", "of", "PI3", "K", "inhibitors", "with", "broad", "or", "narrow", "specificity", ",", "of", "interest", "in", "cancer", ",", "metabolism", ",", "or", "autoimmunity", "." ] } ]
PMC11367939
B 500 nm (left) or 200 nm (right).
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "B", "500", "nm", "(", "left", ")", "or", "200", "nm", "(", "right", ")", "." ] } ]
PMC9429973
All patients had hematological complications in the form of grade 4 leukopenia - 30 patients, grade 4 thrombocytopenia - 28 patients, grade 3 thrombocytopenia – 2, patients grade 2 anemia - 9 patients.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "All", "patients", "had", "hematological", "complications", "in", "the", "form", "of", "grade", "4", "leukopenia", "-", "30", "patients", ",", "grade", "4", "thrombocytopenia", "-", "28", "patients", ",", "grade", "3", "thrombocytopenia", "–", "2", ",", "patients", "grade", "2", "anemia", "-", "9", "patients", "." ] } ]
PMC8584666
After permeabilization, the cells were washed with PBS containing 3% FCS and 0.1% NaN3, and then incubated with Alexa 647-anti-pS536-NF-κB-p65 rabbit mAb (93H1; Cell Signaling Technology, Danvers, MA, USA) and PE-conjugated anti-GPI-80 mAb (3H9) for 30 min at 23–25 °C.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "After", "permeabilization", ",", "the", "cells", "were", "washed", "with", "PBS", "containing", "3", "%", "FCS", "and", "0.1", "%", "NaN3", ",", "and", "then", "incubated", "with", "Alexa", "647-anti-pS536-NF-κB-p65", "rabbit", "mAb", "(", "93H1", ";", "Cell", "Signaling", "Technology", ",", "Danvers", ",", "MA", ",", "USA", ")", "and", "PE-conjugated", "anti-GPI-80", "mAb", "(", "3H9", ")", "for", "30", "min", "at", "23–25", "°", "C", "." ] } ]
PMC11721587
Flag-Fu proteins expressed in Sf9 cells at high MOI were separated into S100 and P100 fractions.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Flag-Fu", "proteins", "expressed", "in", "Sf9", "cells", "at", "high", "MOI", "were", "separated", "into", "S100", "and", "P100", "fractions", "." ] } ]
PMC11228511
We suspect that this result is due low amounts of lamin C within cells (46) or perhaps to reduced binding of the lamin C–specific antibody to fixed samples.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "We", "suspect", "that", "this", "result", "is", "due", "low", "amounts", "of", "lamin", "C", "within", "cells", "(", "46", ")", "or", "perhaps", "to", "reduced", "binding", "of", "the", "lamin", "C", "–", "specific", "antibody", "to", "fixed", "samples", "." ] } ]