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PMC7294030
The bound secondary antibodies on the nitrocellulose membrane were visualized using an enhanced chemiluminescence reagent (Pierce ECL; Thermo Fisher Scientific, Waltham, MA, USA), quantified by densitometry (ImageQuant LAS 4000; GE Healthcare, Chicago, IL, USA) and analyzed using the Scion Image program (Scion Corp., Frederick, MD, USA).
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PMC11684269
Inhibition of ACC1 results in the suppression of Th17 cell formation and concurrently promotes the augmentation of Treg cell and memory CD4 T cell development (45, 47).
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PMC10759659
First, there is spatiotemporal heterogeneity in PD-L expression.
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PMC10747160
But the novel coronavirus nCoV-19 genome expresses several other structural proteins that are packaged with the SARS-CoV-2 virion.
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PMC11785489
For analyses purely focused on cell state, such as dimensionality reduction and clustering, we did not apply TCR-based filtering.
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PMC9429973
The spinal MRI showed a cervical compression in 2 cases (11%), a dorsal compression in 8 cases (44.5%) and lumbar compression in 8 cases (44.5%).
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PMC7351993
Piatkevich et al. have shown directed evolution of genetically encoded fluorescent voltage reporters by using an imaging-based cell picker with a robotic arm for optogenetic research.
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PMC9429973
Results: At the time of data collection, the median time of follow-up was 7.5 years.
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PMC5839394
The trend of up and down regulated proteins compared to untreated cells are shown in Figure 6C.
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PMC10958426
We finally obtained 150 SNVs after removing recorded RNA editing sites in database RADAR and DARNED .
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PMC11591052
Cells were lysed in RIPA buffer (150 mM NaCl; 50 mM Tris-HCl pH 7.5; 0.3% Triton-X100; 1 mM PMSF, with protease inhibitor cocktail) and then sonicated in preparation for immunoblotting.
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PMC11487720
UV/cold shock induced relocation of α-tubulin to the nucleus and nuclear bubbles in damaged cells.
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PMC7153288
A: Representative flow cytometry result showing the apoptotic cells of the A549 cell line treated with 0%, 1.25% and 2.5% (v/v) of the Streptomyces sp. SS1-1 culture broth; B: Increasing of apoptotic cells treated with 1.25% and 2.5% (v/v) of the Streptomyces sp. SS1-1 culture broth compared with that of the untreated cells.
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PMC11271278
We analyzed H3K27ac HiChIP data of the 34 cancer cell lines to determine the origin of enhancers for HAPI genes.
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PMC11785489
To be considered a “TCR-twin,” TCRs from two different individuals were required to match exactly for each of these TCR components.
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PMC11190538
The panelists gave higher color scores to yoghurt fortified with free C-phycocyanin (150 mg) than 300 mg.
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PMC11701801
C, Immunoblot analysis of CBP, p300, H3K27ac, H3, and β-actin expression in SWI/SNF-proficient H2228, SMARCA4/SMARCA2-deficient DMS114, and SS18–SSX fusion Fuji cell lines transfected for 48 hours with the indicated siRNAs.
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PMC9429973
In the MVA, a significantly greater binary SVR was seen among pts with shorter (≤12 mo) vs longer (>12 mo) MF disease duration (odds ratio, 2.1; P=0.022).
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PMC11394730
Anthocyanins are a unique subclass of flavonoids that give various fruits and vegetables their red, purple, and blue color.
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PMC10728200
As a ubiquitin ligase, STUB1 functions by binding to substrates, promoting substrate degradation.
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PMC11627060
d, e, and f) AO/EB staining A549 cells were labeled by AO/EB 24 h after loading of TQ and TQ-BSA NPs es and were examined under a fluorescent microscope.
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PMC4443654
Strong correspondence across cell types was also observed for TAD boundaries, and for the positioning of compartment boundaries, separating A and B compartments (Additional file 1: Figure S2).
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PMC9429973
TAG is approved by the US FDA for the treatment of BPDCN in pts over the age of 2 years with newly diagnosed and relapsed or refractory (R/R) BPDCN; it is also approved by the EMA for 1L treatment of adult pts.
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PMC8193046
Recently, we were the first to report on a novel computer-aided pattern analysis (C@PA) approach for the prediction of potent multitarget ABCB1, ABCC1, and ABCG2 inhibitors, discovering compounds 7–11 (Fig. 2) .
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PMC6892818
Results are presented as % DNA fragmentation relative to positive control (maximal DNA fragmentation) and are representative of two experiments.
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PMC8270637
PD-L1 is the main ligand of PD-1, and its expression is enhanced in a variety of solid tumors.
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PMC9429973
The overall mean (standard deviation [SD]) number of distinct clinical manifestations was 3.7 (3.7), with the highest mean (SD) numbers of clinical manifestations observed in Italy (4.6 [4.7]) and Spain (4.7 [3.7]).
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PMC10957991
The slides were stored at + 4 prior to imaging.
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PMC11615218
The CD4/CD8 ratio in CAR-T cell infusion products is also believed to be an important aspect of the overall therapeutic impact of CAR-T therapy.
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PMC11607638
The SK gene SK encodes a prepropeptide containing two SK peptide sequences in A. aegypti: SK1—FDDY(SO3H)GHMRF and SK2—GGEGEQFDDY(SO3H)GHMRF.
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PMC11599565
Western blot analysis of the stemness features in cells treated with cisplatin and maintained in normal DMEM medium revealed that, SKOV3 cells upregulate Notch1, Vimentin and N-Cadherin while downregulating the epithelial protein E-Cadherin, compared to SKOV3 cells (Western blots from Fig. 3, Panel B), indicating an EMT process.
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PMC8303372
The concentration of GA in each sample was quantified using the calibration curve performed with standard solutions before each analysis.
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PMC6160470
These results indicate conformational change of P-gp similar to the one observed after the treatment with non-competitive modulators or P-gp ATPase blocker sodium orthovanadate.
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PMC9429973
Key exclusion criteria include history of CNS involvement, stem cell transplant or CAR-T therapy <60 days, concurrent anticancer therapy, and clinically significant cardiovascular disease.
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PMC11682525
Consequently, it is conceivable that the reduced HIF1α mRNA levels observed following prolonged hypoxia exposure, were concurrent with an upsurge in HIF1α protein levels.
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PMC8916024
There may also be a similar effect in C. chinensis.
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PMC11633763
Effects of USP22 on osteosarcoma growth in vitro and in vivo. (
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PMC11472639
Considering that exercise-induced leukocytosis is intensity-dependent, it is possible that the increases in immune cell subsets in our study may be more pronounced if an exercise protocol with a greater intensity was used.
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PMC9429973
We used proper validated algorithms that define the demand for radiologic procedures.
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PMC6312945
The non-parametric Kolmogorov-Smirnov test was used to compare the data that did not fit the normal distribution between the two groups.
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PMC11476264
This mechanism has been validated in a variety of cell models, including lung cancer and renal tubular epithelial cells .
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PMC11381192
As anticipated, the high CSI risk group showed reduced levels of immune cell infiltration and immune score, indicative of compromised immune functions.
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PMC11627398
The “limma” R package was used to identify DEGs between control and septic shock groups based on data from the GSE26440 cohort.
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PMC10914904
RIG-I preferentially binds short dsRNAs (< 300 bp) with uncapped blunt ends carrying a 5ʹ-PPP, 5ʹ-diphosphate (5ʹ-PP) groups, and partially methylated 5ʹ-terminal nucleotides, including m7G Cap0 dsRNAs, as well as metabolite-capped dsRNAs such as 5ʹ-CoA-RNAs, 5ʹ-NAD+-RNAs, and 5ʹ-FAD-RNAs [10–17].
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PMC11763111
Every week, a hematology analyzer was used to measure the number of cells in the blood that was drawn from each mouse's tail vein, diluted seven times, and examined.
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PMC11657744
They showed that while CEP89 is essential for ciliogenesis, it is dispensable for cilia maintenance.
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PMC11240571
Programmed death receptor ligand 1 (PD-L1), one of the ICP molecules, is a membrane-bound ligand found on the cell surface of many cell types that are upregulated in inflammation and some oncogenic lesions .
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PMC9429973
PSIP1, a histone mark reader, was primarily found as a dependency factor in MLL-rearranged AML, which was dispensable for normal hematopoietic development, making it an interesting therapeutic target.
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PMC11365427
To construct the xenograft model, each mouse was injected subcutaneously with NCI-H929 cells (5 × 10 cells/0.1 mL injection in PBS).
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PMC11477621
The crude product was purified by silica gel chromatography to give the dark brown solid (360 mg, 70%).
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PMC9429973
The largest difference between reporters and users was “suspicious of” with reporters rating 14% higher, 59% v 45%.
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PMC9429973
These influence activity of ABCC1 and ABCB1.
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PMC8992508
Most of the compounds showed significant inhibition of P-gp transporter and two of them showed an outstanding activity in the doxorubicin resistance reversal.
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PMC9429973
Methods: Thirteen untreated de novo AML patients were enrolled and stratified into the complete remission (CR) (n=8) and the non-CR (n=5) groups.
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PMC10170482
CSs provided mechanical constraints for cellular proliferation: fibers and pores.
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PMC11594074
As the abnormal activity of DNMTs in DNA methylation plays a significant role in carcinogenesis, DNMT inhibition represents a potential target for novel cancer therapy .
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PMC11128598
Finally, we were interested in determining whether the combination of CQ with NHEJi was also effective in the different tumor cell lines under study.
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PMC11473843
Graphical data are mean ± SD.
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PMC7642379
Focusing on the pairwise comparisons between HEK293 cell lines, 38 differentially expressed genes were identified common to all adherent to suspension pairwise comparisons (red column in Figs. 3b, 5a, Supplementary Table S5).
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PMC11591052
In turn, one-carbon metabolism functions as a regulator and a sensor of the cells’ nutrient status through the cycling of one-carbon groups and the transferring of them from donors (serine and glycine) to different acceptor compounds, including the intermediate products of the biosynthesis of nucleotides (both pyrimidines and purines), the amino acid cysteine, S-adenosylmethionine (the main donor of methyl group), and glutathione (the main factor of intracellular homeostasis), etc. .
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PMC10158546
Inoculum was prepared as 10% brain homogenate in PBS via 2 × 30 s sonication bursts and clarified via two 10 min centrifugations at 2,000 xg.
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PMC4659567
Being aware of these facts and the above discussed results of H NMR spectroscopy and ESI+ mass spectrometry indicating a release of the naza ligands from the structures of the studied complexes, it was of great interest to perform the fluorescence quenching experiments on EtBr/ctDNA in the presence of the selected representatives of 1–8.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", ...
PMC11420784
PI3P on the membrane serves as a recruitment signal to recruit autophagy-related protein, such as WIPI, which is required for phagophore maturation.
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PMC9444294
Samples were run in triplicates.
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PMC11352746
The transparency of these experimental designs is critical for the advancement of live-cell imaging instruments for neurite growth quantification assays.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "The", "transparency", "of", ...
PMC9424261
Overall, the results of the in vivo experiments are consistent with our previous in vitro experiments.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Overall", ",", "the", "results", "of", "the...
PMC11792090
In addition to T cells, host NK cells and macrophages can also target allogeneic CAR-T cells.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "In", "addition", "to", "T", "cells", ",", ...
PMC11718109
A Co-expression of VGLUT2-GFP (green), mCherry-synapsin (magenta), synaptophysin (untagged) and VMAT2-FLAG (cyan) in COS7 cells show preference of VGLUT2-GFP fluorescence signals in the synaptophysin-synapsin vesicle clusters.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "B-CellLine", "O", "O", ...
PMC11779960
In the matters arising, Thiele et al. study scRNA-seq data from 12 TCL cases.
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PMC9429973
Despite this disparity, R-HDAC retains a comparable CR rate (41% vs 38%) and 5y OS (64.3% vs 65%).
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", ...
PMC11551844
Briefly, freeze-dried sponges (50.31 g) were macerated in methanol and dichloromethane (v/v, 1 : 1) for 24–48 hours.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ ...
PMC11731614
3Keratinocyte-derived extracellular vesicles significantly alter their protein cargo in painful diabetic neuropathy.
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PMC9429973
As limitations of the study the authors highlight the reduced sample as well as the need for a longer follow-up period.
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PMC9429973
Aims: To establish a follow-up protocol to anticipate and prevent clinical relapsing disease.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Aims", ":", "To", "establish", "a", "follow-up", "protocol", "to...
PMC11754094
P = 0.0017 for TRAC target, P = 0.0003 for PD1 target.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "P", "=", "0.0017", "for", "TRAC", "target", ",", "P", "=", "0.0...
PMC11291490
In this study, we have engineered the TMUV DNA vaccine to co-express prM-E along with dusCD40L (Fig. 3a).
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PMC11731614
One of the challenges with current PDN treatments is their systemic administration and off-target effects (Finnerup, 2015, Attal and Bouhassira, 2021, Shinu, 2022).
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PMC7987994
Numbers above the columns stand for the concentration of measured Zn in µM. The dashed line stands for the normative limit of 70% metabolic activity of the control.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", ...
PMC11781181
Lipid metabolism is closely related to cell sensitivity in ferroptosis.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Lipid", "metabolism", "is", "closely", "related", "to", "cell", "sensitivity", "in", "ferroptosi...
PMC5308810
The peptide was more potent than AMD3100 or CTCE-9908 (Fig 4D and S1 Fig).
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "The", "peptide", "was", "more", "potent", "than", ...
PMC9429973
Admission requirement and presence of risk factors were associated with higher lethality.
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PMC5415764
A wide variety of drug resistance mechanisms was uncovered, such as overexpression of multidrug resistance (MDR-1) gene (P-gp), molecular changes in microtubule, changes in apoptotic regulatory and mitosis checkpoint proteins3940.
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PMC10507284
These suggest that the migration ability of SW982 is significantly reduced after TROAP is knocked down.
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PMC9250505
Given that NPB treatment diminished PARPi-induced CHEK1/CHEK2 phosphorylation and RAD51 foci, which both have an important role in HR, it was therefore determined whether NPB modulated HR.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", ...
PMC11127909
However, BKA decreased C-C3 expression in the 143BR-shEFHD1 + BKA + cisplatin group (Figs. 6D, S13, S15A&B).
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PMC4277423
The deletion mutants contained deletions of −4 to -199 nucleotides.
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PMC5810978
Bovine GIII NoV was quantified with primers SWGIIInewF and SWGIIIrev and SWGIII probe (FAM-) at the following reaction conditions: 50°C for 30 min.
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PMC6901583
Patients (with written informed consent) who went through surgery at the Second Hospital of Jilin University in the period from February 2012 to March 2015 were the source of the 20 GIST samples used in this study; samples were fixed in formalin and embedded in paraffin.
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PMC11682172
The fractions of crossing cells are significantly reduced for MDA-MB-231 and MDA-MB-231-B2M2.
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PMC11742290
Interestingly, low LGR5 protein expression has also been shown to be associated with increased lymph node metastases in CRC tissues .
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Interestingly", ",", ...
PMC10747160
Then, the gene sequence for M78Q Griffithsin with an N-terminal BamHI site and Aspartate to Alanine mutations at positions 30, 70, and 112 was inserted into the pET28b vector between the NcoI and XhoI restriction sites (again from Twist Bioscience).
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", ...
PMC7757104
Osteopontin (OPN) emerges in the interface between chemoresistance and EMT; it is a matrix glycophosphoprotein that serves important roles in tumor cell proliferation and chemotherapy resistance (10–15).
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PMC9817179
After waiting 10 min to allow PFA or PFA/GA fixation to complete, images of the same cells are taken again.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "After", "waiting", ...
PMC11525028
For each targeted gene, two independent target sequences were employed, listed in Table S10.
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PMC10419319
Pharmmapper (https://www.lilab-ecust.cn/pharmmapper/index.html, accessed on 17 October 2022) and SwissTarget (https://www.swisstargetprediction.ch/, accessed on 17 October 2022) databases with the “Homo sapiens” species setting were utilized to predict the protein targets of isoalantolactone.
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PMC11040965
Interestingly, decitabine also enhances miR29b expression in U266 and RPMI 8226 cells, suggesting that low levels of miR29b in MM plasma cells may be due to miR29b promoter methylation (within another self-reinforced epigenetic loop).
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PMC9878562
Our data suggest that LTB4 could be the mediator of the contact-independent enhancement of CXCL12-stimulated migration of ACKR3-deficent cells.
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PMC6617630
Representative images showing MYC, BCL2, and BCL6 rearrangements are depicted in Fig. 1.
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PMC9429973
Localized stage (II) and extented stages (III and IV) were observed in 25% and 75%, respectively.
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PMC9429973
14 patients were diagnosed as double-expression (Table 1).
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