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PMC9429973
Four of these patients are still alive in CR, all after an association of Azacytidine plus Sorafenib with or without DLI.
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PMC11604830
In vivo MRI (7T, Bruker Pharmascan) of a nude mouse bearing a subcutaneous ovarian cancer (A2780 cell line) in the right flank.
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PMC11676674
Images acquired with the widefield microscope were first processed with the Leica Lightning deconvolution tool to improve the signal to noise and consequently facilitate recognition of the nuclei and micronuclei by CellProfiler, and then, the images belonging to the same experimental conditions were analyzed simultaneously by CellProfiler.
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PMC5963610
Cryosections from metastatic lungs were obtained by cryostat (SLEE MEDICAL GMBH Co.) and mounted on plus slides.
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PMC9429973
The starting dose of RUX was 20 mg in 8 pts, 15 mg in one patient and 10 mg in one patient.
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PMC11665584
Comparatively, buccal mucosa had more Dermal Fibroblast 2 and fewer Stromal Fibroblast 1 & 2 populations than skin (Figure 4A).
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PMC11190538
This denaturation disrupts the organisation and transmission of energy among the tetrapyrrole chromophores contained in the protein .
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PMC10529414
Lung cancer is the leading cause of cancer death worldwide .
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PMC11696576
We propose cPLA2α is integral to cancer cell survival in this case by inhibiting iROS accumulation and maintaining epigenetic suppression of programmed cell death.
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PMC7053076
Moreover, the frequent acquisition of multidrug resistance (MDR) phenotype is the additional serious problem in the treatment of AML patients.
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PMC11774747
In line with this assessment, our team observed a strong impact of a high viral load (>5 log) on overall survival, regardless of the involved virus, suggesting that VST should be considered as soon as a patient experience a chemo-refractory viral infection following allo-SCT (6).
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PMC3915447
These results suggest that a combination of SPIO and FA could be a feasible candidate in the development of anticancer drugs.
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PMC11787651
Several proteins also interact with the modifications, known as readers (2, 3).
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PMC11794588
This is common in traditional African medicine.
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PMC11615101
Expression of ALDOA mRNA level in the siTaxilin treated group compared to NS and the recombinant pure α-Taxilin group. (*
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PMC9681981
The analysis of time treatment effects on the expression of ε-toxin gene (P<0.05) The means with the same letter are not significantly different and the means with dissimilar letters are significantly different.
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PMC11770130
The in-house perl scripts were applied to process raw data in fastq format.
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PMC11638255
orally.
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PMC11680578
As compared to PPAR-α also, PPAR-γ level increased 350 times after RGZ treatment (P < 0.001) and 275 times after the combination treatment of PTX with RGZ (P < 0.001) compared to that of control.
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PMC9424261
B Tumor growth curve monitored for 36 days, tumor volume (mm) = 0.5 × length × width.
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PMC11487720
BCD cannot be effectively blocked by chemicals in modulating mitochondrial apoptosis such as staurosporine, betulinic acid, and caspase inhibitors.
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PMC9429973
Results: The majority of the inflammasome-related genes, including IL1B, NLRP3, NLRC4 and S100A9 were expressed in all disease states predominantly by monocytes and M-MDSC (p<0.001).
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PMC9784246
In addition, ultra-performance liquid chromatography-electrospray ionization-mass spectrometry (UPLC-ESI-MS/MS) analysis was used to qualitatively examine the metabolites of the C. macrocarpa leaves’ total extract and its polar solvent fractions (EtOAc fr.,
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PMC11683240
After extensive washing of cells, bound PT was detected by flow cytometry, which revealed a significant, concentration-dependent inhibition of PT-binding to cells by α1AT, regardless of whether a preincubation was performed or not (Fig. 5).
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PMC10094992
Cells were extracted with 1% NP-40 lysis buffer (50 mM Tris-HCl pH 8.5 containing 1% NP-40, 150 mM NaCl, 10 mM EDTA, 10 mM NaF, 10 mM Na4P2O7, and 0.4 mM Na3VO4) with freshly added protease inhibitors (10 μg/mL leupeptin, 4 μg/mL pepstatin, and 0.1 Unit/mL aprotinin).
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PMC11653533
Since the beginning of humans, plants have been the most abundant source of a wide variety of exquisite medications, and they continue to provide any form of unique remedies (Taher et al., 2024, Obonti et al., 2021).
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PMC9429973
In two cases the bone marrow was hypocellular and in two cases the bone marrow was acellular.
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PMC11322866
Briefly, cells (3×10 cells/mL) were treated with GSK126 for the indicated time periods, collected, and then lysed with lysis buffer.
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PMC11319300
An active enhancer chromatin state in B cells situated at the 3′ end of MYEOV, which we call the MYEOV-3′-putative enhancer, has previously been reported (Mikulasova et al., 2022).
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PMC11770199
Data were presented as the mean ± SD. ***
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PMC11661040
The human osteoblast cell line hFOB1.19 was purchased from the Cell Bank of the Typical Culture Preservation Committee of the Chinese Academy of Sciences.
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PMC11342785
The diversity of MT-based structures and functions seen in nature suggests that synthetic MAPs could be engineered to assemble new MT structures with novel functions inside cells, analogous to how synthetic signaling proteins can rewire cellular information processing (7, 8, 9, 10).
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PMC11706297
Thiazole has been pioneering in the design and synthesis of numerous biologically active compounds endowed with potent anticancer properties targeting specific pathways [23–27].
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PMC11739504
Graphical view of domains of Kat2b and designed mutant Kat2b. (
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PMC7612475
Cells were labeled and treated as outlined in G. DNA was visualized with antibodies to CldU and IdU, and the length of CldU and IdU tracts measured at ongoing replication forks.
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PMC11216402
The three groups of GC cell lines, previously clustered based on their chromatin accessibility, were recapitulated using the expression of tissue-specific genes (Methods) (Fig. 2A; Supplemental Fig. S7), showing that the variation in RNA-seq is concordant with that of ATAC-seq.
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PMC11656657
These immunosuppressive PD-L2 + mast cells then suppress tumor-specific CD8 + T-cells in a PD-L2-dependent manner .
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PMC9918897
Measurements were performed using a Thermo Helios-α (Thermo Spectronic, Cambridge, UK) spectrophotometer.
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PMC9429973
Summary/Conclusion: In this analysis, significantly improved efficacy for almost all outcomes was observed with tec when compared with RWCP.
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PMC11365983
PDGFRα signalling in the meninges was assayed using anti-PDGFRα and anti-pY754 PDGFRα antibodies.
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PMC9841531
The mixture was stirred for 4 h at room temperature.
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PMC11033180
D and E) Exploration of the passive and active membrane properties of primary enteric neurons measured in whole-cell configuration. (
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PMC9621239
TOP1, DNA Topoisomerase I. This study provides a comprehensive analysis of the characteristics of HBV haplotypes across the X gene in the liver microenvironment in patients with HCC.
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PMC11680562
These functional groups, derived from the plant extract, contribute to the surface modification of CuO NPs and serve as catalysts during the green synthesis process.
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PMC11742047
5 iterative MS/MS runs with a set collision energy of 20 eV and 5 with a 40 eV collision energy were performed for each cell line.
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PMC11754436
Representative photo of nude mouse tumors. (
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PMC11258145
Comparison of the toxicity of curcumin for HL-60 cells and nerve cells confirmed (as shown in model studies) that the disruption of membrane structure due to the presence of curcumin correlates with damage to native membranes, ultimately leading to cell death.
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PMC10900886
P < 0.05; ***P ≤ 0.001.
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PMC11763111
Cas9-mediated knockdown and lentiviral-mediated overexpression were achieved simultaneously and efficiently, as outlined in Fig. 1B and 1C.
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PMC8632470
Chemicals were N-acetyl-p-benzoquinone imine (NAPQI) (CAS reg.
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PMC10454535
Bortezomib (BTZ), a common treatment for MM, is a proteasome inhibitor that induces apoptosis in MM cells.
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PMC11607321
A special dropout strategy, namely the view dropout layer, was included in MOSA to both improve the model’s predictive power and interpretability.
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PMC11707577
The organization and the number of nematocytes is depleted in animals treated with M13EGFR(TNP) and progressively increase with the irradiation time.
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PMC11344246
At 72 h, media was aspirated, and cells were fixed with 4% paraformaldehyde, washed and stained with DAPI.
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PMC11695546
To ascertain whether the tested had a bactericidal or bacteriostatic action that prevented microbial growth, the MBC/MIC ratios were computed (Huang et al. 2021).
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PMC11682224
A431 and Colon16 cells were seeded in sterilized culture dishes and allowed to grow to confluence.
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PMC9428827
In the present work, not Treg depletion but changes in the balance of CD8/Tregs were achieved, treating the mice with no-alpha mutein.
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PMC10366908
The defucosylated mAbs showed an antitumor effect in xenograft models.
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PMC11746948
Subsequent overexpression of GPX4 in A375 and HT1080 cells significantly mitigated the effects of 2-BP on RSL3-induced ferroptosis (Supplementary Fig. 5g–j), suggesting that 2-BP intensifies cellular susceptibility to ferroptosis primarily through modulating GPX4.Fig.
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PMC11732628
The relationship between miR-490-3p and NUSAP1 in osteosarcoma was further investigated.
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PMC9917080
In cortical astrocytes, on the contrary, such influences promoted the expression of this selenoprotein.
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PMC11704834
In the present study, it was demonstrated that TLE1 expression is upregulated in both human LUAD and LUSC tumors but exerts poor prognostic function only in LUAD tumors.
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PMC10850553
Also, all the dead animals during the observation period were autopsied to assess the onset of SAH.
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PMC9429973
3.
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PMC11594588
Phosphorescent intensities are measured at delay times of 30 μs and 70 μs (with a 30 μs window time) with the ratio of these intensities subsequently converted to phosphorescent lifetimes.
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PMC7351993
As shown in Fig. 1 and Supplementary Fig. 2a, the microfluidic chip plays the following important roles in the RIACS: (i) continuously introducing cells to the SRS microscope, (ii) delivering them from the SRS microscope to the sort point with a predictable latency, (iii) guiding the sorted/unsorted cells to the collection/waste outlets.
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PMC11352746
Of the eighteen immortalized cell lines used in these publications, seventeen were cancerous, and the other was an immortalized mouse hippocampal cell line .
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PMC9341513
In other words, the different rearrangements lead to similar, but not identical, changes to the chromatin structure at CCND1.
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PMC11754291
For the ELISA protocol, α-MSH ELISA testing (LS-F40047 - LS-Bio, MA, United States) was conducted on cell media collected from primary human keratinocytes.
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PMC11739504
Fluorescence staining data revealed that the intensity of IFT components located in primary cilia was decreased in the stable Kat2b deleted cell.
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PMC9429973
Whole genome sequencing (WGS) was performed in one case (diagnostic and transformed tumor and germline DNA samples).
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PMC11782780
We found that EBF3 overexpression suppressed glycolysis capacity in MDA-MB-231 cells, leading to decreased expression of glycolysis products such as pyruvic acid, lactate, and ATP.
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PMC9581083
ex DC.),
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PMC5839394
These peaks are similar to those produced in vivo with E.coli and in vitro by Ag complexed by synthetic DNA oligonucleotides .These results could indicate that in the long-time treatment with AgNPs-EPs increase and/or stabilise the interaction of silver with DNA inducing cell death .
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PMC4659567
Thus, the complexes were studied for their stability in various solvents (MeOH, DMF, and their mixtures with water).
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PMC9895440
For this purpose, T2 cells were incubated at a density of 1mio/ml with the respective peptides diluted in full RPMI supplemented medium.
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PMC11750712
No treatment was administered in this patient.
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PMC9429973
The activity and tolerability of prioritized candidates were further assessed in an integrated pharmacology study in cynomolgus monkeys.
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PMC11013014
C NMR (101 MHz, CDCl3); δ (ppm): 195.5, 164.5, 154.4, 150.2, 134.3, 133.5, 131.5, 129.2, 128.8, 128.0, 127.2, 125.4, 123.0, 116.0, 112.6, 111.3, 111.1, 102.8, 68.4, 23.4, 12.4.
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PMC11628904
This long‐term disruption of epithelial barrier function was associated with increased colonisation of the fungal pathogen C. albicans.
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PMC9429973
During prephase 5 pts discontinued blina; during blina cons-1, 4 pts stopped blina.
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PMC11790599
This unique form of cell death, characterized by excessive vesicle formation, has been labeled “methuosis”, representing a distinct and seldom-reported mode of non-apoptotic cell death (Maltese and Overmeyer, 2014).
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PMC9429973
However, the next day a new hemorrhage appeared in both legs and in left arm with a drop in hemoglobin level and development of a hemorrhagic shock.
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PMC11774112
The ability of the micelles to condense miRNA 34a was determined by agarose gel electrophoresis.
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PMC11740508
We conclude that low dose DNA methyltransferase inhibition can cooperate with low dose PARP inhibition to increase DNA damage predominantly in cells with HRR deficiencies, ultimately producing more cell death than in HRR proficient tumors.
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PMC11604830
The peak corresponding to PFCE‐NE is highlighted with a dashed line.
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PMC11612794
For 3xFLAG-CENP-T immunoprecipitation, cells expressing mScarlet-mAID-CENP-T and 3xFLAG-CENP-T were cultured with 500 μM IAA for 12 h and with 100 ng/mL nocodazole for the last 10h.
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PMC9960565
The prepared compounds were also characterized with FTIR (Figure S46).
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PMC9429973
Summary/Conclusion: We identified new genetic players involved in T-LGLL pathogenesis and disease severity, showing a peculiar GEP of CD8+ STAT3M cases, which are the most symptomatic and treatment-requiring patients.
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PMC11806182
B Identification of the top 12 differential cell subtypes in high miR-330 samples compared to low miR-330 samples.
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PMC11774747
Cooper et al. also used electroporation to transduce MP1-specific T cells with a CD19.CAR plasmid, achieving 96% CAR expression (N not specified) (91).
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PMC11438317
D Interaction of HS1 YF mutants with 3xHA-LP-DACS, as before.
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PMC11394730
It works by inhibiting the phosphorylation of Focal Adhesion Kinase (FAK) that is responsible for activating the signaling pathway to control cell migration .
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PMC11291697
At this age physical examination revealed severe microcephaly (OFC: 46 cm; −4.6 SD) and craniofacial dysmorphisms including long face, hypertelorism, upslanted palpebral fissures, short philtrum, high palate, and prognathism.
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PMC11656380
Therefore, the crude peptides were purified to >95% purity using reversed-phase HPLC (RP-HPLC) on C18 (250 × 21.2 mm) column (BGB Analytik AG, Boeckten, Switzerland) using a gradient system of 0.1% TFA in water as solvent A and 50% acetonitrile/0.1% TFA mixture as solvent B at a flow rate of 4 mL/min with UV detection at 220 nm.
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PMC11759751
The killing ability of CD19 CAR T cells against three B-NHL cells in vitro under different effect target ratio (C) CD19 CAR T cells can effectively kill B-NHL cells in vitro.
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PMC11695623
Euthanasia for cervical dislocation after being photographed.
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PMC11098378
Briefly, the human ubiquitin gene was cloned in frame with IpaH4 to generate a C-terminal fusion protein (IpaH4).
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PMC11576293
4H, Ar-H), 7.7 (s, 1H, pyrimidine–C5-H), 7.5 (d., 2H, Ar-H, J = 8.7 Hz), 7.42 (d, 2H, Ar-H, J = 8.7 Hz), 7.04 (d, 2H, Ar-H, J = 9 Hz), 6.86 (d., 2H, Ar-H, J = 9 Hz), 4.55 (s, 2H, SCH2-), 3.92 (s, 3H, OCH3), 3.81 (s, 3H, OCH3); C NMR (75 MHz, CDCl3) δ (ppm) = 172.1, 164.4, 163.3, 162.2, 158.8, 137, 135.5, 130.1, 129.9, 129.1, 128.9, 128.5, 114.3, 113.9, 107, 55.5, 55.3, 35; HRMS (ESI+) calcd.
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PMC11473750
Differences in cell growth were compared to Control siRNA transfected cells. ***
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