PMCID
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PMC11750165
Our observations imply that a critical role of PEX1 in the cell-fate decision of proliferating neural precursors in the p3 domain during the continuing growth and development of the vertebrate nervous system.
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PMC11650848
We deemed it most appropriate to use cell line pairs that had only one factor different between them, namely the presence of absence of DNA methylation.
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PMC11216402
We identified TFs driving the mesenchymal (RUNX2, ZEB1, SNAI2, AP-1 dimer) and the epithelial (GATA4, GATA6, KLF5, HNF4A, FOXA2, GRHL2) states in GC.
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PMC11607764
Consequently, PTBP1 has emerged as a promising candidate for tumor diagnosis, molecular subtyping, and prognosis assessment.
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PMC11536589
The protein levels of MGST1 in AsPC1-MR cells transfected with different RNA interference (RNAi) molecules.
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PMC11661040
GSK3 agonist: 30µM DIF-3) (*P < 0.05, **P < 0.01, ***P < 0.001) Inhibition of wnt pathway could reverse the effect of CGREF1 overexpression on osteosarcoma cells. (
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PMC11400680
As we are focusing on instance segmentation, our prompt generator (YOLOv9) detects each instance (individual cell or nucleus) and returns the corresponding bounding box prompt for each instance.
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PMC11089031
The most enriched pathways were adaptive immune response, regulation of cytokine production, and T and B cell activation (Figure S1B, orange bars).
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PMC9964654
The results obtained (Figure 5) showed a steady and controlled release of the gold complexes from the micelles.
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PMC9429973
Circulating tumor DNA analysis of MRD at mo 3 and 6 was predictive of relapse (AUC 0.80 and 0.75, respectively).
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PMC9429973
Methods: The primary objective was the comparison of Chronic Graft-versus-Host Disease-free and Relapse-free survival (cGRFS) from time of randomization.
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PMC11707832
Similarly, developing small molecule drugs targeting MAN2B1 might face functional interference and side effects on MAN2A1.
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PMC9429973
Eight of them underwent allogeneic hematopoietic stem cell transplant had head and neck SCC, esophageal cancer, breast cancer, and papillary thyroid cancer.
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PMC11093197
Immunoblotting of total c‐Myc, phospho‐c‐Myc (Serine 62), and phospho‐c‐Myc (threonine 58) proteins. (
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PMC11155445
Compound (161g) was also a potent insecticidal agent with LC50 values of 0.0068 mM and 0.0085 mM against C. quinquefasciatus and A. stephensi (Fig. 87).
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PMC9429973
Aims: The efficacy and safety of bosutinib in resistant and intolerant patients with CML treated with 2 (3L) and 3 (4L) prior TKIs was characterized.
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PMC10719213
Prior to variant calling, we perform realignment for insertions and deletions (indels) based on indels in the input reads with minimum of three indel containing reads.
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PMC2191929
Analysis of the cytotoxic activity of individual transfectant clones showed that cytolytic and nucleolytic activity correlated with granzyme expression but was independent of cytolysin expression within the range examined.
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PMC11763533
Considering that, herein we also analyzed the possibility of using Amy-Luc as a temperature sensor.
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PMC9429973
Table 1 and 2 summarizes the results from the univariate and multivariate analysis for 2 years OS and PFS, respectively.
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PMC11718109
Live calcium imaging of DA neurons (day 50) (H) shows massive spike in calcium levels after high KCL stimulation (H’).
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PMC11794847
Enrichment analysis revealed that differentially expressed genes were primarily associated with mitochondrial components and oxidative phosphorylation metabolic pathways (Fig. 5A–B).
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PMC5443223
Following two washes with washing buffer (0.5% Tween in PBS), the membranes were incubated with horseradish peroxidase-conjugated secondary anti-rabbit antibody (1:3,000; ab6721; Abcam) for 1 h at room temperature.
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PMC11585587
And RA induces apoptosis in MM cells by arresting the cell cycle, inducing the change of MMP, triggering the mitochondrial apoptosis pathway, and regulating the MAPK/ERK cascade signaling.
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PMC11473843
The last treatment with the inhibitors was performed 2–3 h before lung harvesting.
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PMC11650848
We highlighted that this region contains a CTCF site, but further investigation is needed to fully elucidate its complete function.
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PMC11240052
p13 NCBI).
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PMC10154881
Viral titers were determined as the TCID50 using the Reed–Muench method and Vero/N4 cells for rMV‐SLAMblind and rMV‐EGFP‐SLAMblind, as described previously.
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PMC4485648
Accordingly, sulforaphane treatment leads to diminished pH regulation and reduced migration of ovarian carcinoma cells.
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PMC9693627
It was assumed previously that PTCD2 is involved in the maturation of the mitochondrial pre-mRNA of apocytochrome b and ND5 .
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PMC11547972
Amygdalin (5 mg/mL) slightly elevated G0/G1 phase A498 cells and lowered the number of A498 and KTCTL-26 cells in the S-phase.
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PMC3888431
The redundant transcripts of both pipeline results were removed by aligning the reference-based transcripts to the non-reference-based transcripts.
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PMC11678130
The A375 and HaCaT cells were each treated with different concentrations of QUE (5, 10, 25, or 50 µM), 5-FU (5, 10, 25, or 50 µM), and QUE (10 µM) + 5-FU (5, 10, 25, or 50 µM) for 24 h. The 5-FU stock solution was prepared in ultrapure distilled water, while the QUE stock solution was prepared in DMSO.
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PMC7348793
It was originally developed for the propagation of pure populations of recombinant SV40 viruses; however, its uses also includes the propagation of polyomavirus and rotavirus , as well as research on ion channels , apoptosis and autophagy , immunology , cell biology , vitamin , hormone and cell signaling .
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PMC9429973
It is reported that paeoniflorin has great potential in neuroprotection, which can reduce neuronal damage by reducing the secretion of IL6 and other inflammasomes.
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PMC11587541
Spinal Muscular Atrophy (SMA) primarily results from the biallelic deletion of SMN1 exon 7, accounting for the majority of cases.
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PMC9429973
Comorbidity rates did not differ between Group-1 and Group-2, with the exception of hypertension and chronic kidney disease, which were less frequently reported in Group-1 (P=0.0092 and P=0.0179, respectively).
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PMC9429973
RNAseq analyses showed that PIK3IP1 is dramatically upregulated(Fig.1D).
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PMC10411253
He had bilateral cryptorchidism and received an orchidopexy at 6 years of age.
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PMC11576293
For C21H22N2O2SCl (M + H) 401.10850; found 401.10739.
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PMC11684821
CD161 defines a transcriptional and functional phenotype across distinct human T cell lineages (43).
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PMC4005030
The mechanism of the chemopreventive action of protocatechuic acid is mostly associated with antioxidant activity, including inhibition of generation as well as scavenging of free radicals and upregulating antioxidant enzymes.
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PMC11697703
miR-193b and miR-365 gain-of-function decreases dendritic complexity in superficial-layer neurons (A) Experimental approach for miRs loss-of-function (LoF) and gain-of-function (GoF) in deep-layer neuron cultures. (
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PMC9429973
Secondary endpoints include number of pts bridging to SCT, progression-free survival, overall survival, best overall response, duration of response, TAG dose interruptions/administration of IV albumin supplementation in pts with CLS or CLS symptoms, as well as safety and incidence and severity of adverse events of special interest (which include CLS and hepatic, renal, and cardiac events).
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PMC9429973
Those testing positive for BCR-ABL1 were excluded.
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PMC9532503
The resulting nucleotide sequences of both LC and HC were synthetized together with the signal sequences.
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PMC9429973
Secreted by all types of cells, exosomes are nano-scaled particles which carry a molecular cargo that reflects their cell of origin.
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PMC11367939
C Co-expression of ATG9A-EGFP and synaptophysin (untagged) in COS7 cells.
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PMC11655498
Applications of the NLP techniques bear high potential in the assistance of unveiling new targets for EBV reactivation.
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PMC11589157
Total RNA was extracted using a RNeasy Mini Kit (Qiagen 74104, Qiagen, Germany).
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PMC10726501
Invasionassay was applied to detect the invasive capacities of GIST882 cells. (
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PMC10213179
After washing with PBS, cell pellets were lysed directly using a cytoplasmatic extraction buffer (10 mM HEPES [pH 7.6], 60 mM KCl, 1 mM EDTA, 0.075% [v/v] NP40, 1 mM DTT, and protease/phosphatase inhibitors) and vortexed.
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PMC9429973
Statistical analysis of medians between cohorts were compared by the non-parametric Mann-Whitney (Graphpad Prism).
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PMC10900886
Representative gating strategies for flow cytometry analyses. (
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PMC11711871
G, H Representative Western blot analyses of G adrenal (GR, n = 6; GR, n = 6) and H aortic rat sEH protein abundance (r sEH, 64kD, GR, n = 5; GR, n = 4) on a NSD (upper panel) or HSD (lower panel) and their quantification (left panels, NSD; right panels, HSD).
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PMC11655498
Furthermore, advancements in various new technologies can provide valuable insights into the critical mechanisms underlying EBV reactivation from multiple perspectives.
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PMC5839394
R1 and R2 corresponded to the Rayleigh scattering peaks.
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PMC9429973
Image: Summary/Conclusion: Substantial and clinically relevant improvements in QoL were consistently observed with PEG across both EORTC QLQ-C30, total LASA and FACIT-Fatigue scores and clinically significant improvements in fatigue and dyspnea are highly relevant to the symptomology of PNH.
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PMC9429973
Pts received 3 cycles of Ibr, followed by 12 cycles of I+V (Ibr 420 mg/d orally; Ven ramp-up to 400 mg/d orally).
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PMC10452486
Carreno and Hansen confidently claim that the binding of exogenous peptide ligand can influence the expression and half-life of B2m-free HLA variants at the cell surface.
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PMC11269933
This study discovered that phosphorylation of the conserved PK Ser11 site in mollusks (Bivalvia and Gastropoda) significantly enhances its enzyme activity.
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PMC11653552
To this aim, miR-590-3p was found to be released at elevated levels in the culture medium of MM cell lines panel compared to the levels from a lymphoblastoid cell line (LCL).
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PMC11629192
The CD19 B cells was measured in percentage.
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PMC11746942
In this study, we found that S1PR3 expression was closely related to the severity of psoriasis.
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PMC11674441
Most breast cancers are detected at an early stage, and the risk of recurrence can be reduced through surgery and drug therapy to prevent recurrence, but there are still many cases of recurrence .
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PMC5673060
Blue coloured proteins are from the cluster list and grey are added STRING interactors.
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PMC2196094
To obtain the complete sequences of these proteases, the ∼500-bp insert fragments were used as probes in screenings of the RBL-1 cDNA library.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ ...
PMC11323699
Fig. 6TAX2 efficacy in a metastatic mouse model of ovarian cancer.
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PMC11754784
CEM, K562, Ramos and PMI-8826 cells were used as the model cancer cells.
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PMC11767725
Elevated androgens, such as testosterone and dihydrotestosterone, stimulate sebaceous gland hypertrophy and sebum overproduction.
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PMC10745221
The methionine addiction of cancer cells is known as the Hoffman effect.
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PMC4277423
The resulting vector was designated as pRRL-Cas9-HA-NLS.
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PMC11752740
Fig. 9The effects of pistachio hull extract (E), carfilzomib (CFZ), and their combination on Caspase3 gene expression levels in SK-BR3 and MCF10A cell lines.
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PMC11588085
We examined the stemness composition of OVCAR3-KRAB cells with XIST KD using mRNA SC-derived embryoid bodies index (EB-index), which reflects the transcriptomic signature of embryonic bodies made of pluripotent SCs, mesoderm, endoderm, and ectoderm progenitor cells (52).
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PMC11453018
E RT-qPCR analysis of senolytics target gene expression following 24 and 72 hours of iP300w (1µM) treatment.
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PMC11394227
In CHO-PAb and CHO-PAb-QS cells, antibody folding efficiency, ER stress markers, ER and Golgi apparatus contents, anti-death ability, and redox status were measured and evaluated to reveal the mechanism of HsQSOX1b and survivin co-expression affecting the production efficiency of CHO cells.
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PMC9000591
Accordingly, oxidative stress can exacerbate the inflammatory process, which mediates most chronic diseases, including cancer.
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PMC11656048
EdU proliferation assays indicated that shikonin inhibits the proliferation of MG63 and 143B (Figures 1G, I) in a dose-dependent manner.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "B-CellLine", "O", "B-CellLine", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], ...
PMC9429973
M. Sciumè, A. Bosi, G. Ceparano, C. De Magistris, F. Serpenti, P. De Roberto, G. Galassi, F. Onida, N. S. Fracchiolla Hematology Unit, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milan, Italy; Università degli Studi di Milano, Milan, Italy Background: Combination of venetoclax and hypomethylating agents (HMAs) has become a standard of care in adult patients with acute myeloid leukemia (AML) who are aged≥75 years or who have comorbidities that preclude use of intensive induction chemotherapy.
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PMC10243817
Oral glucocorticoid and intranasal steroid sprays were discontinued at least 3 months and 1 month before surgery, respectively.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Oral", "glucocorticoid", "and", "...
PMC11750161
The cAMP homogeneous time-resolved fluorescence (HTRF) assay kit was purchased from Cisbio (Codolet, France).
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PMC9429973
Accordingly, patients with submono-nucleosomal cluster had a lower PFS.
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PMC11672142
C NMR data are reported by chemical shift (ppm).
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PMC10196713
SOD activities were monitored by using SOD Assay Kit-WST (Dojindo Laboratories, Inc.), according to the manufacturer's instructions.
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PMC11366496
Based on univariate and multivariate Cox regression analyses, RNF122 mRNA expression level was correlated to WHO stage; hence, it was found to be an independent predictor of unfavorable survival outcomes in glioma patients (Table S2), indicating that RNF122 might be a potential glioma biomarker.
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PMC11544122
2SNX9 binding to the carboxy-terminal tail of CXCR4.a–d SNX9 binding to the carboxyl-terminal tail (CT) of CXCR4 was assessed by GST pulldown.
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PMC9583612
In addition, GHGSat conducted satellite measurements concurrently at the enrolled assets when weather conditions allowed.
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PMC11750712
Few studies to date have reported on use of CAR T in PTCL.
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PMC10605143
In particular, the MYC oncogene could be responsible for higher levels of HSF1 expression in tumors (according to the Human Protein Atlas, the expression levels of both genes in tumors correlate well with each other).
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PMC11798926
How might SynDIG1’s localization and trafficking impact synapse development and plasticity?
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PMC11281366
HIV-1 is protected from CML via the incorporation of several complement-regulatory proteins, such as CD46, CD55 and CD59, into viral particles .
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PMC11357960
The following fluorescently-conjugated antibodies were used for cell phenotypic analysis: CD45- PerCP (BD Bioscience, USA; Cat#652803), CD3-FITC (BD Bioscience, USA; Cat#349201), CD4-APC-Cy7 (BD Bioscience, USA; Cat#557871), CD8-PE-Cy7 (BD Bioscience, USA; Cat#557746), CD19-APC (BD Bioscience, USA; Cat#652804), CD56- BV510 (BD Bioscience, USA; Cat#563041), CD69-Alexa Fluor 700 (BD Bioscience, USA; Cat#560739).
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PMC11286266
Lower panel: Grey rectangles in top panels indicate the subcellular polyclonal population re-sorted to analyse single ATF6αΔ clones in parental and CRTΔ clones.
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PMC11225860
The accumulation of stagnant and apoptotic cells in G2 will fail to progress through mitosis and into G1, resulting in declining G1 populations.
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PMC8097906
Based on our previous anti-cancer studies in breast and bone cancer, we performed an anti-tumor screening with the focus on the anti-metastatic properties of two selected medicinal plant extracts: Vincetoxicum arnottianum Wight and Linum usitatissimum and four plant-derived compounds: genistein, daidzein, matairesinol and secoisolariciresinol on the pediatric RMA cell line RH-30 [21–23].
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PMC10197932
AS shown in Figure 5.2 B, a small decrease in cytosolic Calcein-AM uptake was observed and most Calcein was retained in the viable lymphocytes (89.13% of Untreated (UT) cells, about 58.55% for treated (T) cells, and 54.33% for cells 2T).
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PMC11767817
5637 cells, at a density of 15 × 10, were seeded in a 96-well plate and incubated overnight.
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PMC11569208
Multi-step and single-step growth curves of 786-0 infected with VSV∆51-NTC or VSV∆51-shACP2 (MOI 0.01 or 1).
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PMC8193813
The boiler stacks are about 20m apart and reach over 20m in height above ground level.
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PMC11683240
B–C, PT (10 ng/ml) and different concentrations of α1AT or the respective amount of solvent (H2O) were added directly to CHO-K1 cells in FCS-free medium and incubated for 4 h at 37 °C.
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