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PMC11412721
Here, we show that mice lacking the Trp53 alternatively spliced (AS) exon, thereby expressing the canonical p53 protein but not isoforms with the AS C-terminus, have unexpectedly lost a male-specific protection against Myc-induced B-cell lymphomas.
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PMC9429973
Rapid reduction of serum free light chain (FLC) was seen at the first cycle of PVD therapy.
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PMC11763764
In the ovarian cancer cell line OVCAR8, we demonstrated that treatment with this antibody at 10 μg/mL for 24 h downregulated the levels of EGFR and FGFR4 (Figure 4B).
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PMC11758416
Otherwise, you risk cells dying as they sit and wait to be recorded which makes it more difficult to account for targeted killing by cytotoxic T cells.
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PMC6442998
For immunostaining, slides were brought to RT, permeabilized and blocked for 1h in 0.01M TBS pH 8.2 containing 0.1% Triton X-100 (Sigma, Saint Louis, MO, USA) and 10% normal horse serum (NHS).
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PMC11743975
To quantify mitochondrial aggregation, we employed image entropy (Shannon, 1948) to measure the chaotic degree of mitochondrial distribution at different time points.
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PMC9429973
The prognosis of most patients with PMBCL is very good.
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PMC10470467
Here, based on the random forest algorithm, we identified 6 key necroptosis-related genes (NRGs) and used them to construct an NRG signature with excellent predictive performance.
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PMC7338939
However, our results determined that in the long-term treatment and after eliminating the effect of DAPT, melanoma cells became more aggressive with increase in both sphere and colony formation abilities.
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PMC11594588
One of the effects of the above changes is platelet apoptosis.
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PMC10965315
In a brief procedure, harmaline (37.5 and 75 μM) was applied to the A2780 cells for 24 h. Following centrifugation of the culture media, 50 μg of total protein was electrophoresed at 12% concentration on an SDS polyacrylamide gel with 0.1 mg/mL gelatine as the substrate.
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PMC11634794
Transwell results further substantiated a significant reduction in the number of LAMP1-overexpressing cells penetrating through the filter membrane compared to the control group.
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PMC11297139
Additionally, ARID1A WT that is capable of LLPS showed the ability to bind EWS/FLI1, whereas the PrLD Y/S mutant failed to bind (Fig. 6a), suggesting that the interaction between ARID1A and EWS/FLI1 relies on LLPS.
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PMC9341513
Figure 7A shows results from a simulation where chromatin state and DHS data for GM12878 cells were used as input, but the hg19_u266 genomic insert rearrangement was included (as depicted in the cartoon).
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PMC10747160
To avoid this, researchers have found that mutating M78 to Glutamine alleviates these issues, making M78Q Griffithsin an easier and more feasible candidate to use for clinical trials .
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PMC11656380
For the mitochondrial membrane potential assay, cells were incubated with 40 or 80 μM of Tat-Ram13 for 2 h at 37 °C and added with JC-1 assay reagent (Cayman Chemical, Ann Arbor, MI) for 10 min.
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PMC11612626
Compound A treatment significantly increased the presentation of pan–HLA-ABC (W6/32) by ∼1.6-fold (P = 0.0003) in RPMI-8226, ARH-77, and U266 cells (Fig. 7A; Supplementary Fig. S5A).
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PMC11143482
The strain of mice used was (NOD.Cg-Prkdscid Il2rgtm1Sug/Jic Tac) NOD.SCID gamma, aged between 8 and 10 weeks, weight between 20 and 30 g. These animals were bred in CIEA Japan and purchased from BSC Malaysia Sdn Bhd.
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PMC6442998
We found that both PDE3A mRNA and protein level were lower in GIST48 cells as compared to GIST882 cells, while SFPQ, a known transcription factor for PDE3A , was also repressed in GIST48 cells, in line with the lower PDE3A level in these cells.
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PMC11596967
After cooling for 10 min, the samples were transferred to a 96-well plate and the absorbance was read at 532 nm.
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PMC10748106
Geniposide was inactive against cervical cancer cells, but Genipin was cytotoxic against the HeLa cell line.
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PMC11678448
The KEGG enrichment analysis indicated that the DEGs were significantly associated with pathways such as cytokine-cytokine receptor interaction, viral protein-cytokine, chemokine signaling pathway, and IL-17 signaling pathway, among others (Figure 5B).
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PMC10728535
Characteristics of the samples applied in the meta‐analysis and further investigation.
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PMC11638255
Of note, copper chelation resulted in increased numbers of CD8 effector T cells yet revealed a marginal decrease in NK cells, the latter contrasting with our previous report.
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PMC11473750
Cells were then treated with 100 µg/ml CHX for up to 30 min followed by immunoblotting of total cell lysates with anti-RNF121 or anti-MYCN antibodies.
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PMC8973085
Potent biological activities such as antileishmanial, antimicrobial, anti-inflammatory, anticancer, and antiplatelet agents were also observed from thienopyridine and their derivatives.
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PMC8753818
Then 100 ng of pLEX-JRed vector was transfected into the CHO cells in triplicate.
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PMC10734950
The pellets were resuspended in 200 μl of PBS containing 5 μl of NucView488 caspase 3/7 substrate.
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PMC11394227
Cells with a fusion of 80–90% were digested with 0.25% trypsin (Solarbio) and cultured in a ratio of 1:4, with the medium changed every two days.
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PMC11525028
Representative IHC images (left) and quantification (right) of IHC showing the expression of NAT10 and Ki-67 in tumors (n = 8 per group).
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PMC9220170
In this work, it was shown that SELENOM-KD and SELENOT-KD under conditions of ER stress also did not significantly affect the proliferative properties of A-172 cancer cells, even when cells were exposed to 5 µg/mL SeNP for 24 h. Previously, we showed that 5 µg/mL SeNP caused an increase in the expression of mRNA of these selenoproteins in three of the studied cancer cell lines, including A-172, by more than three times , and therefore we expected the opposite effect.
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PMC9014716
Subsequently, the DMEM was replaced with 100 µl of serum-free DMEM with the addition of 10 µl of CCK-8 solution and incubated under an atmosphere of 5% CO2/95% air for 3 hours at 37°C.
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PMC11180402
Values are mean ± SD from three trials. *, #,
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PMC11794588
The peaks that were collected were put via gas chromatography.
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PMC11486946
Representative images obtained by single-molecule imaging are displayed.
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PMC11155445
Compounds (68a–e) photoluminescence properties were studied by Tudor et al. in solid-state samples and dichloromethane solution form.
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PMC9429973
On the basis of enrichment analysis, it was found that AP2M1 may be related to intracellular receptor signaling pathway.
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PMC11371747
The colon epithelial cancer cell line CaCo2 has a high PCFT expression and is widely used as a model for intestinal drug uptake.
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PMC11676674
The acquisition was performed by selecting in focus and single cells, discarding events not representing individual cells.
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PMC9878278
Cell viability was evaluated in infected SC at different days after infection using CellTiter 96 AQueous One Solution kit and shown as percent cell viability compared to mock-infected cells. (
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PMC9429973
Median DOR was not reached ([NR]; 95% CI, 0+ to 23+ months) and 6 patients (51%) had response ≥12 months.
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PMC9429973
The preliminary results demonstrate that the combination therapy is relatively safe with anti-tumor activity.
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PMC11348300
In contrast, the 3 h and 6 h time points show PpIX concentration reaching its maximum in the blood (Fig. 4d), indicating that PpIX has leaked out of the cells through the ISF to reach the bloodstream.
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PMC5963610
Supplementary Figure 1J exhibits the lung weight in an earlier cut off the experiment by day 40.
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PMC11389534
Furthermore, compound‐8 contributes to combination treatment with PD‐1 blockade in vivo, revealing a novel modulator of PD‐L1 and highlighting UFMylation as a possible immunotherapy target.
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PMC11597291
The A549 cells were treated with 10 μg/mL nCGO-PEG/FITC and nCGO-FITC samples for varied time periods (1, 5, 24, and 48 h).
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PMC6642070
We then checked protein levels of well-characterized upstream regulators of glycolysis including hypoxia-inducible factor 1- α (HIF 1-α), c-Myc, phospho-Akt and TP53-inducible glycolysis and apoptosis regulator (TIGAR), and there were no significant changes of these proteins (Supplemental Fig. 3).
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PMC11683240
Cells were left untreated as further control.
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PMC7342409
Due to the small sample size (n=5) of each group, the standard deviation between groups was large, and there was no statistical significance (Figures 3B and 2B).
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PMC10547921
However, due to its narrow treatment window and toxic side effects such as neurotoxicity and gastrointestinal reactions due to the lack of selectivity, it has been clinically prohibited from direct use in human treatment.
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PMC9592219
miR-22-5p inhibited the expression of TOP2A by binding to the 3′UTR of TOP2A mRNA.
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PMC11740508
A M231 xenografts.
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PMC7215832
We previously showed that a high PCFT expression is associated with a longer survival of patients treated with the anti-folate PMX and demonstrated that treatment with the hypomethylating drug DAC increased PMX uptake in mesothelioma cells .
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PMC11790599
Breast cancer is the most prevalent malignancy among women globally.
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PMC11803918
With very few standard treatment options, the median survival is around three to 11 months.
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PMC10957991
In addition, one should consider the interplay between various mechanosensitive ion channels, including the Piezo family channel proteins.
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PMC9429973
The 4 groups did not differ with respect to pts’ gender, type of AML, cytogenetics, and performance status.
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PMC11541241
In Δasd4 mutants, the TOR pathway was activated, and exposing these mutants to the TOR inhibitor RAP could restore appressorium development.
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PMC11578343
Although viscous G″ continued to increase with respect to zone length (Figure 3E, iv), elastic G′—the parameter to which mz‐visco‐NPS is most sensitive—plateaued with a zone length of 6 mm (Figure 3E, iii).
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PMC11791478
The Kruskal–Wallis Dunn or Mann–Whitney U test was used for comparisons of in vivo tumor growth inhibition.
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PMC7723249
The opening of all junctions (PMA and EGTA) increases the level of infection of CCHFV compared to the non-treated and only PMA-treated cells, which suggest that most of the receptors/co receptors of CCHFV are localized on the basolateral side of polarized cells.
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PMC11461874
The resultant protein sample was diluted 5 times with 100 mM NH4HCO3 solution and digested with trypsin (Sigma) at 1:100 [wt/wt] by incubation at 37 °C for 18 h in the dark.
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PMC5916734
Meanwhile, there are certain limitations to the cultivation mode: unlike CHO, this cell line requires fetal bovine serum to be present in the medium and can grow in a normal manner only under adhesion conditions.
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PMC9429973
Leukemia & Lymphoma.
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PMC10788478
Elevated LGR6 expression is correlated with poor prognosis of esophageal cancer, , breast cancer, cervical cancer, and ovarian cancer.
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PMC11765243
Our results indicated higher Akt and Erk activation in p110αKD-T thymocytes activated with anti-CD3 plus anti-CD28 antibodies, as shown by their increased phosphorylation (Figure 4A,B).
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PMC9424261
Based on this finding, we considered whether HERPUD1 could increase the ubiquitination of GSS by affecting the ubiquitinating enzyme associated with GSS.
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PMC11753435
DLD1 cells per well were seeded on a white-bottom 96-well plate (1 × 10 per well; Thermo Fisher Scientific, 136101) 24 hours before transfection.
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PMC5839394
The composition of the AgNPs-EPS materials are differently rich of Ag(I)-NPs or Ag(0)-NPs.
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PMC4270159
The breast cancer cell line MDA-MB-435 has been shown to be mislabeled; it is in fact identical to the M14 melanoma cell line (Rae et al., 2007; Chambers, 2009; Holliday and Speirs, 2011).
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PMC11543885
CRITICAL: The amount of trypsin required can vary depending on the initial material and the efficiency of the IP-Streptavidin process.
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PMC11297139
Plasmid and A673 cell mixture were electroporated by Neon Transfection System with 1650 volts, 10 ms pulse length, and 3 pulses condition following the supplier’s instructions.
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PMC11411131
MeHg can interact with and deplete Sec. Usuki and colleagues previously reported that MeHg reduces GPx1 expression through NMD-mediated suppression of gpx1 mRNA.
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PMC8708099
Two main types of IBD can be distinguished: ulcerative colitis (UC) and Crohn’s disease (CD).
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PMC11540664
Second, the study included a combination of systemic regimens, including lenvatinib and sorafenib, which may differ in prognostic outcomes depending on the choice of the drug.
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PMC9429973
ORR was 95% in those (17/18) received ZD regimen more than 2 months.
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PMC11402217
However, the role of DKK1 in OS remains uncertain.
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PMC11767725
By addressing systemic metabolic dysfunction and microbiota-mediated pathways, precision strategies are paving the way for improved patient outcomes in dermatologic care.
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PMC11718817
For the coculture with BMDMs, previously polarized macrophages with activated or not activated NLRP3 inflammasome were adjusted to 8 × 10 cells and resuspended with the tumor cells in 45 µL DMEM medium before adding 15 µL Corning Matrigel.
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PMC9599726
Approximately 85% of GISTs are driven by somatic gain-of-function mutations of the gene encoding the tyrosine kinase receptor (RTK) KIT (CD117) .
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PMC11543935
The EP further led to a change in viability.
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PMC11715644
Through live imaging over time (the frame that monolayer reaches the wall of PDMS channel is defined as 0 h), we observed that cell density is relatively higher in outside regions (unconfined) compared to inside microchannels, as shown in representative phase contrast and nuclei images (Fig. 1B), and cells aggregate near the entrance of the microchannels (Fig. S1A, ESI†).
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PMC11098378
Moreover, crystallography and cell cultures studies reveal Ceg10 to encode a putative cysteine protease function that is required for arbovirus rescue.
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PMC11449273
H1299 cells and its transfectants were maintained in minimum essential medium (MEM) supplemented with 10% fetal bovine serum (HyClone Laboratories, USA), 1% penicillin-streptomycin and 5 μg/mL kanamycin (complete MEM, CMEM).
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PMC10522430
This study aims to explore the impact of G. applanatum crude polysaccharide (GACP) extract by assessing its effects on cell viability, levels of proinflammatory cytokines such as TNF-α, IFN-γ, IL-2, and IL-12, and levels of proapoptotic markers including caspase-3 and caspase-9, as well as the percentages of necrosis and apoptosis in the HeLa cell line.
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PMC11092382
Few studies have explored the impact of sophocarpine on tumors through its modulation of inflammatory cytokines.
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PMC11740207
NDSCs activate several signaling pathways that regulate their proliferation, differentiation, and responses to environmental cues: Wnt/β-catenin regulates SD and enhances proliferation; NOTCH controls differentiation and helps maintain the SC state; TGF-β plays a role in maintaining SC homeostasis by regulating the transition from quiescence to active differentiation and influencing the immunomodulatory abilities of these cells; PI3K/AKT/mTOR controls proliferation and survival (mTOR overactivation may reduce regenerative capacity, but modulating this pathway supports their repair functions); and hypoxia-inducible factors (HIF) facilitates adaptation and function in low-oxygen niches, promoting survival and maintaining pluripotency in stressful, oxidative conditions.
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PMC10606998
The dose efficiency can be increased by using radiosensitizers.
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PMC11075223
ITS2, as a non-coding nuclear DNA, has the characteristics of easy sequence amplification, high success rate and strong universality.
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PMC11731614
N = 3 male biological replicates for both RD and HFD.
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PMC11047729
From the 1980s until 2001, it has been the first line of treatment for CML—right now, the combination of IFN-α [EB1] and other drugs is being tested .
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PMC11507371
Specifically, p65 can inhibit Nrf2 by competitively interacting with the transcriptional co-activator CREB binding protein (CBP) and by promoting the recruitment of histone deacetylase 3, a co-repressor, to ARE .
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PMC11381192
TIDE score was calculated for each patient to assess the immune evasion capacity of MM cells.
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PMC11742670
The MSE and MAE comparison for the testing and training datasets to predict IC50 on the three different cell lines are given in Figs. 7 and 8, respectively.
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PMC4395774
The concentration of IFN-γ was determined by using enzyme linked immunosorbent assay kit obtained from R&D Systems (Minneapolis, MN) per the manufacturer's instructions.
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PMC11541409
A. Schemes of inactivated and activated wild-type oncoprotein (KIT/PDGFRA).
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PMC11755519
The differences between the effects of T24 and J82 exosomes may be due to the reduced amount of LINC02470 transported in J82 exosomes compared to T24 exosomes.
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PMC11411004
RNA was extracted with chloroform, isopropanol, and 75% anhydrous ethanol, and then, total mRNA was reverse transcribed to cDNA using oligo-dT. The qRT-PCR amplification assay was executed utilizing a reaction system comprising 10 μL of stock solution, 4 μL of 5 × Evo M-MLV RT reaction mix, and 6 μL of RNase-free water, resulting in a final volume of 20 μL. The ΔΔCt method, calculated from the number of cycles (Ct value) at which the fluorescence signal reached a threshold during PCR amplification, was used to determine relative gene expression.
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PMC11763764
Tumors were collected and fixed to prepare the formalin-fixed paraffin-embedded (FFPE) tissue samples.
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PMC4695073
b. CCRT, including concurrent chemoradiotherapy only or plus induction chemotherapy.
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