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OTAR3088
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BioData-CellLine_annotated

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PMC11650848
A CCCTC-binding factor (CTCF) binding site also overlaps this region and may influence expression.
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PMC8345486
Another model in which we investigated the anticancer properties of the compounds was 3D spheroids.
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PMC9429973
W. Zhang, J. Liu, Z. Shi, L. Yang, B. Li, Z. Xiao State Key Laboratory of Experimental Hematology; National Clinical Research Center for Blood Diseases; Institute of Hematology & Blood Diseases Hospital, Tianjin; Chinese Academy of Medical Science & Peking Union Medical College, Beijing, China Background: The JAK2V617F...
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PMC11687663
In this molecular docking study, the protein target selected was human peroxisome proliferator-activated receptors (PPAR-δ), specifically in complex with the agonist AZ 242 (PDBID: 1I7G) .
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PMC10698968
Graph shows the ratio between the number of invading cells and the number of seeded cells compared to 143B results (fold change).
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PMC11656657
The expression of GD2 antigen on tumor cell lines and patient’s biopsy tissues was assessed with an anti-GD2 mAb (clone 14.g2a, BD Biosciences, USA).
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PMC10914904
Finally, understanding the mechanisms of vtRNA biogenesis is important.
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PMC9917080
SELENOT is similar in structure to SELENOM.
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PMC9684669
Ovarian cancer cells were plated at 3,000 to 5,000 cells/well in 100 µL of complete culture medium in white, solid-bottom 96-well plates and incubated overnight at 37°C with 5% CO2.
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PMC10470467
Different expression level of 6 key genes in the tumor/normal tissue (A) and high- / low-risk group (B).
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PMC11583690
Specifically, at a concentration as low as 5 μg/mL, the cytotoxicity of BPMO@DTX increased significantly compared to free DTX (with p < 0.01), and this difference became more pronounced at higher concentrations.
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PMC7582629
In the last decade, Raman Spectroscopy (RS) was demonstrated to be a label-free, non-invasive and non-destructive optical spectroscopy allowing the improvement in diagnostic accuracy in cancer and analytical assessment for cell sensing.
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PMC10669966
Immunoblotting and cell viability assays were used to study LPS cell lines and their sensitivity to PDE3A modulators.
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PMC11695623
The expression of DCUN1D5 in cancer tissues and normal tissues. (
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PMC11757230
Given these conflicting effects of MSCs on metastatic development and tumor cell growth, we investigated the effects of cADSCs on the metastasis of canine lymphoma cells in our xenotransplantation model.
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PMC9954564
This procedure is repeated for each data piece, with classification and prediction errors obtained across all 10 runs.
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PMC11727145
The results of the H&E staining of DED construct histological sections (Figure 4) supported those of the macroscopically illustrated MTT assay.
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PMC8633974
Further analysis shows that stimulation led to up to 100 (JLat) to 300 (U1) fold increases in RNA production.
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PMC11472639
This is unsurprising given that previous research in healthy humans has shown increases of 24%, and 119% in CD3CD8 T-cells following low and vigorous intensity cycling, respectively (Campbell et al., 2009).
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PMC10813895
Extensive studies have characterized ELF5 as a crucial factor in early embryonic development, breast development during pregnancy, and BC development.
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PMC11040965
The assay was performed in triplicate.
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PMC11767817
Since each compound was active at different concentrations, we selected the following doses: 20 µM, 8 µM, and 2 µM for curcumin, 2a, and 2a-B, respectively.
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PMC11483468
The results showed that the percentage of dead cells increased with the final concentration of 200 µg/mL GST-TAT-LPTS39 protein for 72 h, and the death rate reached 18.45% (Figure 4A).
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PMC11682172
The SK-BR-3 (SKBR3) cells were isolated in 1970 from pleural effusion of a breast adenocarcinoma patient.
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PMC6835428
In this review, we concentrated on the description of the therapeutic potential of siRNAs and polymer-/lipid-based delivery systems for OC.
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PMC7433824
The spots were then dehydrated with 100% acetonitrile and dried.
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PMC9436459
Therefore, this kind of Diels–Alder product has trans–trans and cis–trans types, both of which are found in natural products.
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PMC9429973
Tolinapant, in combination with the HMAs, azacytidine and decitabine, displays a very high degree of synergism, in vitro, even at very low concentrations of both HMA drugs.
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PMC9429973
It was an early complication (median onset 28 days).
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PMC9429973
K. Geissler, Z. Koristek, T. Bernal del Castillo, J. Novák, G. Rodriguez Macias, S. K. Metzelder, A. Illes, A. Nagy, J. Mayer, M. Arnan, M.-M. Keating, J. Krauter, M. Lunghi, N. Stefano Fracchiolla, U. Platzbecker, V. Santini, Y. Sano, A. Oganesian, H. Keer, M. Lübbert Clinic Hietzing, Vienna, Austria; University Hospi...
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PMC10854447
Compounds 7l and 7k exhibited good fitting (Figure 7A–C), where the phenyl triazole scaffold bi-stacked between Phe918 and Phe92 forming pi–H interaction with Leu840.
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PMC10957991
Such biophysical alterations can lead to abnormal activation of the mechanosensitive machineries and subsequently promote cellular changes linked to cancer progression.
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PMC5941560
However, of the ∼ 408 thousand Jurkat SNVs in COSMIC, we uncovered over 383 thousand (94%) matching single-nucleotide variants.
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PMC11507371
Non-malignant cells seem to possess a more pronounced antioxidative reserve capacity before approaching their threshold, as evidenced by HaCaT’s significantly heightened antioxidant capacity after CAP stimulation relative to A431 cells (Figure 4B).
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PMC9429973
Median OS was 51m, 24.6m, 15m, and 9.9m in the IA MRD(-), Lo + VEN MRD(-), IA MRD(+), and Lo + VEN MRD(+) groups, respectively.
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PMC9436459
Sorocenol F (74) obtained from S. bonplandii was a prenylated derivative of 73.
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PMC9351137
The localization pattern of BAF53a and Tip60, as well as that of MKLP1 were affected, while no effect was seen for SRCAP and Aurora B. B The effects found with ZM447439 treatment are summarized in the graph.
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PMC10870498
ATC cells were seeded onto the 6-well plates.
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PMC6163708
Malignant melanomas are sensitive, when taken into account also our previous results (Figure S1).
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PMC11543885
Alternative: this step is only for anchorage-dependent cells, like HEK293T, that weakly adhere to the plastic plate bottom.
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PMC11323699
Two different sequential treatments were performed.
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PMC11361748
Then, total RNA was converted to complementary DNA (cDNA) using iScript cDNA Synthesis Kit (Bio-Rad #1708891).
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PMC11486946
The regions of the plasma membrane and cytoplasm were identified as the area within 5 pixels inside the cell boundary, which was determined with Cellpose 3.0, and the other pixels within the cell, respectively.
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PMC11225860
Combining Aurkin A plus alisertib disrupts endomitosis while promoting apoptosis.
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PMC8540692
Curcumin can cross the blood-brain barrier, thus yielding therapeutic benefits within the CNS .
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PMC9096373
The differences of γ-H2AX and apoptosis in the APA + RT group were most significant compared with the other groups (Table 1).
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PMC11320084
At present, the clinical treatment of tumor metastasis is limited and has little effect.
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PMC11794847
Differences between two groups were analyzed using the Student’s t-test, while one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test was employed for multiple group comparisons.
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PMC8864075
A total of 6.0 × 10 A549 cells were cultured in medium without PS and were plated in six-well plates.
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PMC10823017
Renal Cell Carcinoma (RCC) is the most common type of kidney cancer (85%).
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PMC9429973
The Table outlines AEs.
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PMC7342409
Effect of imatinib and chloroquine on apoptosis of GIST882-ir cell line (after 48h). (
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PMC9429973
To study PI3Ki drug class activity in idelalisib-refractory CLL 3.
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PMC7612475
.
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PMC10729469
Identifying endogenous disease reactive Tregs or developing them though immunization is further complicated by low Treg precursor frequencies and lack of effective expansions.
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PMC11562028
PI staining was then performed using a cell cycle kit.
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PMC7351993
Unfortunately, these technologies predominantly rely on fluorescent labeling for cellular phenotyping, an indirect measure of molecules in the cell, which has several critical limitations.
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PMC11499381
The mVenus signal was evident for both C-terminal and N-terminal fusion constructs with both CLR and CTR based receptors.
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PMC11607638
Downregulation of SK and SK receptor (SK, SKR) expression was found to increase, but microinjection of the SK peptides (SK1, SK2) to decrease blood meal intake.
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PMC9220170
On the other hand, SELENOM-KD did not significantly affect the expression patterns of Se-containing glutathione peroxidases and thioredoxin reductases, as well as key markers of the three ER stress signaling pathways.
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PMC10595704
PS-NP induced apoptosis after 96 h of treatment, whereas they were unable to induce senescence at the size and concentrations used.
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PMC11267830
Within 48 h post-indisulam administration, fluorescence microscopy revealed that a significant proportion of the T-ALL cells died (Fig. 2a).
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PMC8193046
The five multitarget ABCB1, ABCC1, and ABCG2 features [(i–iv) F1–F4: aromatic/hydrophobic; and (v) F5: acceptor] as reported before are depicted (A), to which compound 23 was aligned to (B).
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PMC10204952
Further studies are warranted to identify the most important secretome targets of S1P in adipocytes and illustrate the mechanism on how these target proteins affect S1P treatment of TNBC.Sphingosine-1-phosphate (S1P) is the last metabolite in the highly conserved sphingolipid metabolism pathway.
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PMC10728200
Significance denoted by: ns not significant, *P < 0.05, **P < 0.01, and ***P < 0.001.
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PMC11607321
Fourth, the diverse size of the omic multi-omic datasets may lead to some datasets dominating during training, diminishing the model’s generalizability and explainability.
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PMC11599565
The first characteristic observed in this primary cell culture is the existence of islands of nested cells, which depict a distinct morphology from the surrounding cells.
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PMC11790599
Classification of cell lines used in our study.
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PMC11078693
Carefully aspirate away the supernatant from the cell pellet.j.
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PMC11098378
To obtain C-terminal 5XHis-tagged PSMC1, human PSMC1 was cloned into pET21a by restriction cloning with NdeI/XhoI and expressed in E. coli BL21in the presence of ampicillin.
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PMC6461034
A “kinome” analysis data table is generated from table 3 by: ○Removing redundant rows with different UniProt gene symbols linking the same phosphopeptide to the same HGNC‐mapped gene symbol.○Filtering for rows with phosphopeptides that are derived from protein kinases.
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PMC10048572
Thirty-five of 381 Jurkat-O1 subclones (9.1%) had LOH for one marker.
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PMC7887261
Human cell lines and reagents.
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PMC9429973
Primary study objectives include safety, maximum tolerated dose (MTD), recommended Phase 2 dose (RP2D), and preliminary efficacy.
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PMC11786767
To quantify the fluorescence signals, we drew a perpendicular line across the chromosome periphery of sister chromatid pairs that had fully condensed during mitosis and performed a line scan analysis of DAPI signals along that line (Fig. 2A).
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PMC11209164
DMF also increased Ad5-GFP-associated fluorescence in MRC5 (peak ~ 100 µM), A549 (peak ~ 110 µM), HT1080 (peak ~ 100 µM), and HepG2 (peak > 250 µM).
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PMC11487720
The COS7 cell thickness (height) is approximately 4.5 μm, and the bubble thickness less than 2 μm (n = 10; p < 0.0001 versus time zero controls; Student’s t-test).
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PMC11537649
The correlation between miR-152-3p and NEAT1 was assessed by Pearson correlation coefficient test. (
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PMC6450504
bPEI-coated PEIPOS, 0.5% (by moles), was used to complex siSCR at different ratios of nitrogen/phosphate (N/P), present in PEI-PE and siRNA, respectively.
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PMC11481779
We have developed the HiCDiffusion model, which provides high metrics as obtained by other encoder-decoder-based algorithms and significantly improves the predictions’ human-readable quality.
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PMC11565047
RNA was then sent to sequencing at the Next Generation Sequencing Platform of the CRCHUQ-Université Laval (based in Québec City) using an Illumina NovaSeq 6000 (paired-end, 100 bp sequence length, depth of 20–25 M reads requested).
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PMC9429973
Results: Ide-cel was associated with improved HRQoL vs BM at all evaluated time points for the QLQ-C30 global health status/QoL domain (Table).
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PMC11802855
We selected “B cell proliferation” and “osteoclast signaling” as target pathways and conducted signaling pathway enrichment analysis.
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PMC9429973
Urinary immunofixation showed the presence of Kappa free light chains and serum free light chain assay showed an increased Kappa/Lambda ratio.
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PMC11612508
Optical micrograph (magnification 200 ×) of A172 cell line treated with GO/CS/IO magnetic microspheres containing TMZ in the absence (e) and presence (f) of the magnetic field after 24 h compared to untreated control (d).Fig.
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PMC10975211
These analogs acted like their parent AMPs, easily penetrated bacterial cell membranes, and effectively were bound to DNA in vitro .
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PMC9884169
After 24 hours, the cells were incubated with the diluted LPO probe (10 μMol/ml, (Dojindo, Japan) for 40 min in the dark in a 37°C incubator, taken out and washed with PBS 3 times, and then imaged by a fluorescence microscope (Olymbus IX83, Japan).
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PMC1971384
Dual binding between tumour cell surface antigen and MTX was demonstrated by the ability of the hybrid antibody to bridge between tumour cells and MTX as MTX-HSA conjugate, reaction here being detected by flow cytofluorimetry.
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PMC11676674
The methanolic extract was then filtered through quantitative filter paper (ArtiGloss, particle retention in the range of 12–15 μm) and concentrated by rotary evaporation (BUCHI R-210) at 40 °C for 30–50 min.
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PMC11545737
These include human pathogens, the most studied of which are Salmonella and Shigella spp. ,
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PMC11705484
Representative hematoxylin‐eosin (H&E) staining of liver and kidney tissues from the mice after treatment with NC, DDP, CMP1, CMP9 or Thiram. (
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PMC11741906
cDNA was synthesized from 2 μg total RNA using miScript II RT kit (Qiagen, Germany) for 1 h at 37 °C.
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PMC9454178
The mRNA level was calculated via GEO2R.
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PMC10955424
Ascites fluids were obtained from women with ovarian cancer or liver cirrhosis undergoing peritoneocentesis for clinical reasons in Ziv Medical Center (Safed, Israel) Helsinki ethics committee approval #0044–19-ZIV and Bnai Zion Medical Center (Haifa, Israel) Helsinki ethics committee approval #0112–20-BNZ and bought f...
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PMC11762280
p-MLKL phosphorylated MLKL.
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PMC11773391
Besides, abnormal levels of mismatch repair (MMR) proteins were also reported in metastatic melanoma , downregulation or impaired MMR might be considered a cause of acquired resistance to TMZ and other methylating drugs (together with MGMT).
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PMC9429973
Preclinical studies showed that bendamustine and pomalidomide had higher penetration rate of cerebrospinal fluid.
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PMC11750165
A–C) Expression vectors for PEX1 and J2ICD were transfected into P19 cells.
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PMC2196094
To estimate the total number of closely related members of this subfamily in the rat genome, a Southern blot analysis was performed with genomic DNA from Sprague Dawley and Wistar rats.
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PMC9429973
No infusion toxicity was observed.
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