PMCID
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PMC9884169
Besides, combined with the above sequencing results, it was speculated that MCT4 affected cell ferroptosis by regulating oxidative stress.
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PMC7433824
Among these are the neuroleptic agent thioridazine, the antidiabetic agent metformin, and the cholesterol‐lowering agent bezafibrate, which are already being safely used for indications other than cancer.
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PMC11726183
The initial analysis of RMSD for 23 and Staurosporine complexes disclosed that the 23 complex had large conformational changes leading to 10.5 Å RMSD at the end of simulation when compared with Staurosporine complex having 9 Å as the RMSD as depicted in Fig. 6A.
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PMC11394730
Genistein downregulates hypoxia-inducible factor-1α (HIF-1α), therefore inactivating glucose transporter 1 (GLUT1) or/and hexokinase 2 (HK2), which in turn suppresses aerobic glycolysis and mediates apoptosis .
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PMC11635087
Post reaching this maximum, the fluorescence decreased gradually, reflecting the ensuing electron transfer processes.
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PMC11461833
Red indicates genes that behaved differently than expected.
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PMC11248911
MM/PBSA (Molecular Mechanics/Poisson−Boltzmann Surface Area) calculations were done using g_mmpbsa, a GROMACS tool used to calculate an estimated binding affinity.
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PMC11683130
Some serpins, including SerpinA12, SerpinB2/3//7 play multiple roles in skin barrier function and pathogenesis of psoriasis.
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PMC11471176
Consistent with this counterintuitive finding, Nair et al. established that elevated ACE2 expression is an unfavorable prognostic marker in HER2-enriched tumors .
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PMC11469524
By this simple modeling approach, an acyclic network with only activating interactions was derived which contained no feedbacks and lacked quantitative information.
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PMC11684297
for 75 µg/ml, and 1178 a.u.
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PMC11472639
Clonal plasma cells were phenotyped as CD38CD138CD45CD19 with light-chain restriction and polyclonal plasma cells were phenotyped as CD38CD138CD45CD19 with polyclonal light chains.
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PMC11519788
SPOP deficiency led to the STAT3 protein stabilization and the increased CCL2 expression and secretion, thereby facilitating macrophage recruitment and polarization.
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PMC11144200
We calculated the target cells binding characteristics of LPS-RGD-Nb36-DOX, which are essential to its efficiency and specificity.
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PMC9429973
No trends for decreases in bone mineral density were observed.
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PMC11025866
These two genes are involved in glutathione metabolism and the redox status of the cell.
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PMC9844987
The right subpanels display box-and-whisker plots of the differences in these metrics across promoters split by variability quartiles (central band: median; boundaries: first and third quartiles; whiskers:+/-1.5 IQR.). (
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PMC10582049
Gene expression of ADH1B CAF markers across all identified subpopulations of cells. (
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PMC11562028
However, the prepared Re-CDs have good solubility in water.
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PMC11543885
Incubate and rotate the magnetic beads 16 h at 4°C.
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PMC6337547
Phenylalanine and tyrosine are precursor compounds of the phenylpropanoid metabolic pathway, and their biosynthetic pathways constitute two parallel branches of this pathway, which involve five rate-limiting enzymes .
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PMC11585587
No.SR8010) with a purity of 99.58%, was wholly dissolved in dimethyl sulfoxide (DMSO) and stored at -80 ℃.
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PMC11409031
To compare the survival rates between these groups, Kaplan-Meier analysis was utilized.
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PMC11764090
Thus, plant responses to cold stress are complex and multidimensional, involving signal perception and transduction.
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PMC9429973
Methods: Response to ASA in patients with MPN was evaluated by a platelet light transmission aggregometry induced by arachidonic acid.
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PMC4112127
As shown by amplification in MSP reactions, CpG sites in the targeted island (Figure 1A) were methylated in genomic DNA from all five biliary cancer cell lines, but not in DNA from the H-69 immortalized normal cholangiocyte cell line (Figure 1B).
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PMC11568601
OE Biotech Co., Ltd. (Shanghai, China) carried out the RNA-seq and analysis.
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PMC11411131
MeHg, Pb, Co, Cd, Mn, and Sulf were dissolved in DDW for chemical stimulation.
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PMC11740508
Colonies were fixed/stained 30 min with 0.5% crystal violet/20% methanol and images qualitatively acquired with a BioRad ChemiDoc imaging System (BioRad®), with color correction and exposure time alteration to allow optimum colony identification using Image Lab software (BioRad®) and quantitatively assessed by ImageJ (FiJi).
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PMC9473868
Recent studies showed that BAI not only inhibited nasopharyngeal carcinoma (NPC) cells proliferation and induced NPC cells apoptosis but also blocked the PI3K/AKT and p53 signaling pathways in CNE2 cells .
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PMC10237474
Expression of the GOI in these vectors is driven by constitutive promoters.
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PMC10988808
G Representative images and bar graphs of wound healing assays of Caki-1 and 786–0 cells after overexpression CCDC25 (**p < 0.01) We conducted RNA transcriptome sequencing analysis on 786–0 cells overexpressing CCDC25 and normal 786–0 cells to explore the potential mechanism of CCDC25 in renal cancer.
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PMC11240448
First, we applied an 8-fold cross-validation (CV) for the SVM classification of cell states after removing data related to one cell line.
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PMC9429973
EPO levels in this group were elevated in 7.8%, normal in 88.2% and decreased in 3.9%.
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PMC4485786
Counts were decay-corrected to adjust for the radioactive decay of I between measurement time points (n = 2). *
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PMC11565047
A, B) Gene Set Enrichment Analysis (GSEA) diagrams and heatmaps of the Epithelial-Mesenchymal Transition (EMT) (A) and interferon alpha response (B) gene sets following treatment with R1881 (a synthetic androgen) compared to control (Ctl). (
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PMC11637284
Right, Immunofluorescence of extracted flagella of the same cell line probed for TAC60ΔC283-myc (red) shows the TAC60 variant gets integrated into the TAC.
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PMC11727638
Ahmed et al. reported that HIF-1α might regulate CD20 expression in lymphoma .
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PMC9429973
The quantitative analysis of the combined methylation of the studied genes was carried out with the calculation of the one-sided Fisher exact criterion (p-value) and the frequency of false discoveries (FDR) (q-value) was calculated using the Benjamin-Hochberg procedure.
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PMC5386478
The highly selective TG2 inhibitors, 1–155 (cell-permeable) and R281 (non-cell-permeable), were designed and synthesized as previously documented.
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PMC5854676
The incubation time was set to 14 h, and cells were incubated in total darkness or after receiving a suitable dose of 660 nm laser radiation.
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PMC10523483
Cell viability of cells with or without H1.2 knockout as measured by MTT assay at 24 h, n = 5 to 6. (
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PMC8708099
no. 3420-1H-6, IL-13—cat.
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PMC11655536
CI < 1, CI = 1, and CI > 1 indicate synergistic, additive, and antagonistic effects respectively.
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PMC11354225
A-431 cells were cultured in Dulbecco’s minimal essential medium (DMEM) high glucose (HyClone), supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin.
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PMC7057940
Expression of the FLI1-EWS mature transcript expression was demonstrated in patient 1; however, the translocated chromosome encoding FLI1-EWS is likely lost in a small subset of cells, contributing to the genetic heterogeneity of the sarcoma (22).
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PMC11770130
By contrast, the physical activity and fecal energy between Ntsr2 BKO mice and control mice were comparable (Supplementary information, Fig. S1m, n).
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PMC11306331
One can envision future studies where MBP is used to dissolve transcription factor condensates at a single transcriptionally active locus of interest to specifically alter their presence or composition and monitor real-time effects on gene expression or genome spatial organization.
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PMC9429973
NK cells were characterized by flow cytometry in 8-12-week-old mice.
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PMC11459296
After 3 days, CD69 and CD80 expression was significantly higher in stimulated B cells compared with unstimulated cells (Figure 2D).
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PMC9429973
The final model consisted of hsa-miR-409-3p (OR 0.61, 95%CI: 0.37-0.99, p=0.0480), hsa-miR-328-3p (OR 0.33, 95%CI: 0.13-0.87, p=0.0254), age (OR 1.13, 95%CI: 1.03-1.23, p=0.0096) and R-ISS 3 (OR 2.91, 95%CI: 0.63-13.47, p=0.1723).
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PMC9429973
The analysis of TRB repertoire was performed in 22 D0 and 10 corresponding D120 samples of HABMF patients that had undergone IST and we compared the diversity and clonotype composition with the nonHABMF patients as well as the BM graft samples and NBPB.
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PMC10222971
Nevertheless, it did not show a significant cytotoxic activity on melanoma cells, with 74.5% of viable cells and 24.7% of apoptotic cells.
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PMC9429973
M. Jabbar Al-Obaidi, A. Chew, C. Liu Haematology, Chelsea and Westminister NHS Trust, West Middlesex University hospital, Isleworth, United Kingdom Background: Vaccination against COVID-19 commenced in England in December 2020, most haematology patients iwere included in the first wave as they are identified as extremely vulnerable.
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PMC11789597
For adhesion to BMSCs and co‐culture assays, 1 × 10 BMSCs were seeded in 96‐well plates overnight.
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PMC11770130
Then Ntsr2 AKO mice were treated by pair feeding, a method to normalize the food intake of control and Ntsr2 AKO mice and validate its significance for the physiological changes.
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PMC11766309
Pan-cancer analysis of immune cell infiltration and immune checkpoints. (
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PMC8658661
Moreover, increased IGF2 expression is associated with a poor prognosis of the course of cancer because it supports both the proliferation and migration of tumor cells .
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PMC9429973
Aims: The multicohort phase 1/2 MK-4280-003 study (NCT03598608) evaluated the safety and efficacy of favezelimab (MK-4280), a humanized Immunoglobulin G4 LAG-3 inhibitor, plus pembrolizumab (a PD-1 inhibitor) in patients with R/R hematologic malignancies.
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PMC10125312
Cytotoxicity was assessed by means of mitochondrial activity, whereas uptake was quantified with two-photon microscopy.
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PMC11731614
Immunoblotting revealed a robust GFP signal in the epidermal cell lysate from our EV-reporter mouse line.
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PMC11171319
Based on similar oncogenic features compared to the already recognized seven human oncoviruses and the fulfillment of all the hallmarks of cancer, even the most recent ones described in 2022, the definitive classification of HCMV as the eighth human oncovirus has to be taken into account and will lead to new therapeutic approaches that are actively needed to curtail cancers, especially adenocarcinoma and glioblastoma of poor prognosis.
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PMC9429973
The presence of LAC in the plasma of late period CCP donors and APLA in the early period donors can be explained by the changed in SARS-CoV-2 viral variants between the two time periods (wild type alpha and non-alpha variants in the early and late periods, respectively).The age of plasma donors in early CCP period was older than the age of those who donated in late period, reflecting the fact that the alpha variant was more common in elderly people during the early pandemic or that there was a careful selection of CCP donors during the course of the pandemic.
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PMC11792740
Transcription factors and epigenetic regulation play essential roles in T-cell depletion.
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PMC8750422
This study supports our results, highlighting the oncogenic function of GPR87 in lung adenocarcinoma.
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PMC11591030
The RNA integrity was evaluated via horizontal electrophoresis on 1.5% agarose gel (Solarbio, Beijing, China, Cat.
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PMC11011837
As indicated in Table 1, a significant difference appears in the total lipid content among the cell lines, especially between Jurkat and CCRF-CEM on the one hand and Molt-4 and Molt-16 on the other.
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PMC10440586
However, in the non-responding group, there was no significant difference in the expression level of CD99 before and after administration (Fig. 5B).
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PMC9429973
To discover immune pathways and molecules that may serve as targets for immune therapy.
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PMC11472639
A CONSORT flow diagram of the recruitment and experimental completion of the study.
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PMC9777812
In this study, with luciferase assay experiment, bta-miR-106b was validated to target the 3′-untranslated region (UTR) of bovine CDKN1A, thereby regulating its expression.
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PMC11638255
While the integration of anti-disialoganglioside (GD2) antibody (e.g., dinutuximab) into maintenance therapy has been demonstrated to substantially improve survival and is now a standard of care, nearly half of patients remain refractory or develop treatment resistance.
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PMC9429973
The odds of death in ICU grow up to ~2 times if the patient has a Glasgow score of less than 15.
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PMC11471157
Image showing spheroids of HaCaT cells and NIH/3T3 cells stained for the cytokeratin marker CK AE1/AE3.
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PMC11530949
The annexin V/propidium iodide (PI), nitric oxide (ROS) assay, and DCFDA assay kits were provided by Roche (Germany).
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PMC8143558
The type of extract also impacts on the hemolytic activity of the samples, as much as the type of plant does.
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PMC11314247
HO-AAVPA likely induces apoptosis and cell cycle arrest via this pathway.
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PMC9429973
No significant differences in survival were observed according to the number of cycles (P=0.095).
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PMC10812665
The ratio NADH/NAD was therefore severely compromised, indicative of reductive stress.
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PMC6222635
The graph of HPLC was shown in Figure 6.
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PMC10933092
The Cox model of 29 transcripts identified by Lasso regression stratifies the risk of BLCA.
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PMC10900886
If only ~1%–8% of PBMC B cells become infected, viral replication will potentially occur below the detection limit of our titration assay (2); EV-D68 fails to replicate in PBMC B cells.
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PMC2118063
After 5 min at room temperature, the suspension of purified B cells was decanted into collection tubes.
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PMC9031474
An overload of Ca ions is known to induce ROS release from the mitochondrion.
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PMC11706776
C Plasma membrane depolarization measures in A-673 cells treated with the SK1 channel activator GW542573X (N = 50).
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PMC11474209
Our knowledge of the molecular pathways underlying the development of chronic ailments, including cancer, neurological disorders, cardiovascular disease, and respiratory disease, has been enhanced by recognizing the adverse effects of these pollutants on human cells.
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PMC9429973
Results: Subjects with antithrombin deficiency had 35% reduced antithrombin activity compared to controls (p<0.001).
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PMC9429973
Furthermore, evidence-based guidelines on the best method of pain management during BMAB are lacking.
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PMC10545062
In contrast, other groups have focused efforts on the computational modeling aspects of ACC systems and have leveraged applied animals.
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PMC11612626
Biologic signaling systems exhibit vast, nonlinear responses to T cells that may be manipulated to better discriminate between cancer cells and healthy tissues (59).
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PMC9429973
In 63% of these patients, clinical relapse was observed, treatment was started due to aggressive biochemical relapse in the rest.
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PMC11495567
Antibodies specific to Phospho-BRAF (Ser445) (Cat. #
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PMC11285179
Our results in Fig. 3M also suggested CCL28 can promote retinoic acid accumulation in pericytes in vitro.
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PMC9429973
S. Okabe, Y. Tanaka, A. Gotoh Department of Hematology, TOKYO MEDICAL UNIVERSITY, Tokyo, Japan Background: Myelodysplastic syndromes (MDS) is indicated by bone marrow failure and increased risk of transformation to acute myelogenous leukemia (AML).
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PMC11750696
Mice with a deficiency in ATF4 in their T cells exhibit reduced proliferation rates (15).
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PMC9429973
We extracted data regarding reasons for not performing the biopsies from the computerized system, according to the treating physician.
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PMC11544122
not significant.
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PMC9966931
Control treatments consisted of 1% (v/v) of DMSO with PDA medium.
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PMC11621493
Data are presented as mean ± SD.
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PMC9429973
The REL transgene is followed by an IRES to allow YFP marker concomitant expression.
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