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OTAR3088
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BioData-CellLine_annotated

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PMC6627640
Our findings are the first to implicate epigenetics in the chemosensitivity of TGCTs.
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PMC11739484
Furthermore, MMP-induced changes in the tumor microenvironment, such as increased interstitial pressure or altered stromal interactions, may further impact drug efficacy.
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PMC11679033
Exposure to a single CIT at the tested concentration significantly lowered lymphocyte viability, while single CBD treatment led to a lower rate of lymphocyte death.
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PMC11026382
The slurry was then transferred to a disposable polypropylene column (BioRad).
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PMC11348300
Transient hypoxia can be induced in tissues through tissue palpation.
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PMC11686380
Scale bar, 50 μm. (
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PMC5810978
SaVs have been detected from humans, pigs, mink, dogs, sea lions, bats, chimpanzees, rodents, and carnivores .
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PMC9429973
The degree of splenomegaly was independent risk factor for both myelofibrotic transformation (hazard ratio [HR]: 7.75; 95% confidence interval [CI]: 1.87-21.10; P = 0.005) and OS (HR: 6.70; 95% CI: 1.89-23.72; P = 0.003).
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PMC11521786
To evaluate the antitumor effect of the polyclonal antibody response generated by the HER3-based vaccine candidate, IgG isotype PAbs were purified from the sera of rabbits immunized with Mv-HER3, which contained HER3-specific antibodies (see Supplementary Figure S7 ).
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PMC11247842
The outcome of a mastitis infection is influenced by many factors, including the resistance of the host, the pathogen(s) in question, the interaction between pathogen and host, and the environment .
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PMC11472639
EDTA-treated blood was then centrifuged (Heraeus Biofuge Primo R, Thermo Fisher Scientific, Loughborough, UK) within 2-h of collection at 2000×g, 4 °C for 15-min for the isolation of plasma.
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PMC11786767
D and E: Reduced Ki-67 phosphorylation disrupts chromosome and microtubule organization.
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PMC11089031
A The expression value was calculated by the TNMplot web tool (www.tnmplot.com, 18 July 2022) using TCGA RNA-seq datasets with available data for tumor and adjacent normal tissues.
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PMC11746948
All other cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, USA).
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PMC11345828
Suspension cells were plated immediately before compound addition.
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PMC9848491
In order to investigate the relationship between the predictive signature and prognosis of patients in UCEC classified based on age and grade, UCEC patients were grouped into internal training cohort and validation cohort.
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PMC7823217
These include interactions with the surrounding matrix, host cells, and receptors or ligands of both cancer and stromal cells.
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PMC11574271
To understand how miR-659-3p regulates the RON expression in more detail, we inserted the RON 3’UTR containing a miR-659-3p target site and a mutant sequence lacking the target site into the downstream of a firefly luciferase coding sequence region.
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PMC11739504
Western blot data showed that overexpression of Kat2b restored the decreased acetylation level of α-tubulin in Kat2b silenced cells at 6 h and 24 h after serum starvation.
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PMC11783017
However, as the concentration of H2O2 increased, the weight loss of the hydrogels gradually escalated.
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PMC10671321
The beads were then washed and incubated with conditioned medium containing ectoLRP4 at 4 °C overnight.
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PMC10440586
The landscape of TME heterogeneity in gliomas.
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PMC11627060
The results are presented as mean ± standard deviation, with a significance level of p < 0.05.
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PMC9429973
Methods: We generated five SRSF2 Mut (P95H) isogenic cell lines with CRISPR/CAS9 system and performed high throughput drug screen with 3988 compounds in a single dose.
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PMC11643491
These mechanical processes involve intricate interactions between the physical properties of particles, including size, shape, and stiffness, and the biomechanical properties of the cell that are affected by membrane fluidity, viscoelasticity, and cytoskeleton dynamics.
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PMC10887351
In specific, β3-ARs control various vascular functions and regulate pathological angiogenesis in models of retinal vascular proliferation.
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PMC10243817
Obviously, these issues require further explorations in future.
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PMC11594641
Densitometric analysis was performed using ImageJ software, version 1.53k.
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PMC3813807
The present study showed that miR-373 is upregulated in gastric adenocarcinoma tissue and gastric carcinoma cell lines when compared to normal gastric tissues.
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PMC9960565
As can be seen in Table 6, except for the bidentate G1.5(COOPt(NH3)2)8 metallodendrimer, all other metallodendrimers showed RF values significantly lower than cisplatin (RF = 36), and the monodentate G1.5(COOPt((NH3)2Cl)16 is nearly similar to cisplatin.
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PMC11763764
For cell cycle analysis, cells were collected and washed with ice-cold PBS, and then fixed in ice-cold 70% ethanol at −20 °C for 30 min.
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PMC11621493
Protein expression was confirmed by Western blotting analysis (supplemental Fig. S3A).
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PMC7039683
Genes associated with the top-scoring 0.1% bin of tiles are enriched for those in this cluster (hereafter, mouse periderm) (hypergeometric p-value=0.044) (Li et al., 2019).
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PMC11544771
Together, our results reveal that the combined treatment with CPX and PRE inhibits NSCLC cell proliferation by arresting the cells in the G1/G0 phase of the cell cycle.
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PMC11706776
B. Normalized and relative EWS::FLI1 (left panel) and KCNN1 (middle panel) gene expressions in A-673/TR/shEF cell line after doxycycline treatment (1 µg/mL) (N = 5).
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PMC11001582
As polyGR and polyPR are consistently damaging across model systems, we focused on generating polyGR and polyPR knock-in mice (Fig. 1a).
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PMC11476666
The present study suggests that DHS may hold similar promise in combination therapies, given its superior performance in reducing spheroid growth and stemness marker expression compared to RSV.
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PMC11765988
The specific equilibrium between M1 and M2 macrophage polarization in the microenvironment of each neoplasm is critical for tumor progression .
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PMC11337594
n = 3) (E, F) A375 cells were exposed to lower doses of TER (0.1, 0.3 and 0.5 µg/ml) for 48 h. The cells were labeled with PI and detected by flow cytometry Inhibition of cell proliferation by TER is associated with a decrease in CyclinA2. (
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PMC11791432
Combined with the results of bioinformatics analysis, it is suggested that ANXA1 has a role in promoting the progression of glioma and is associated with poor prognosis of glioma.
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PMC9684669
GAPDH was used as the loading control. (
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PMC11703992
D ScPagwas TRS score of different T cell clusters.
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PMC9429973
Response was better in late relapse (64 vs 21%, p 0.001) and in patients relapsed after a prior autologous transplant (61 vs 33%, p 0,02) in KRD arm.
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PMC9429973
Median time to onset of any grade CRS and ICANS were 4 and 7 days, respectively.
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PMC11476264
Lung cancer is the leading cause of cancer death and has one of the lowest 5-year survival rates among all cancer types .
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PMC11684821
Recognition of cancer cells showed dependency on the K43 residue.
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PMC11507371
Indeed, we demonstrated that with an escalating CAP exposure time, the hydrogen peroxide, nitrite, and nitrate concentrations significantly and gradually increased (Figure 4A,C), while the antioxidative capacity of malignant A431 decreased in the same fashion (Figure 4B).
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PMC11547972
Varying SFN preparation techniques may also influence anti-tumor potential.
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PMC9429973
Image: Summary/Conclusion: The percentage of immune cells and expression of immune checkpoint proteins on immune cells from BMs of MDS were distinct from that of healthy control samples.
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PMC11366496
An internal control, IgG, was utilized to ensure the specificity of the experiment.
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PMC8073654
In our previous study on the anti-inflammatory effects of phenylbutenoids from Z. cassumunar , four compounds, trans-3-(3,4-dimethoxyphenyl)-4-[(E)-3,4-dimethoxystyryl]cyclohex-1-ene (11); trans-3-(4′-hydroxy-3′-methoxyphenyl)-4-[(E)-3′′′,4′′′-dimethoxystyryl]cyclohex-1-ene (18); (E)-4-(2′,4′,5′-trimethoxyphenyl)but-1,3-diene (14); and (E)-1-(3′,4′-dimethoxyphenyl)but-1,3-diene (1) demonstrated inhibitory activities with IC50 values of 2.71, 3.64, 14.97, and 20.68 μM, respectively (celecoxib; IC50 0.52 nM), in the COX-2 inhibitory assay by measuring prostaglandin E2 (PGE2) production in lipopolysaccharide (LPS)-stimulated mouse macrophage RAW264.7 cells.
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PMC11459296
These populations, particularly B cells, NK cells and monocytes, were minimally found in the PB-MNC model.
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PMC6102174
Detailed LCMS analyses on the active part showed the presence of a series of chlorogenic acids.
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PMC9429973
We isolated the lowest 20% of cells fluorescing with CellROX probe (ROS low) and the highest 20% (ROS high) by cell sorting, and analyzed cell cycle, engraftment in mice and RNA sequencing to identify deregulated pathways.
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PMC9792775
The A-204 and HT-1080 cells were seeded and operated as above, then cells were cultured in the 5% CO2 atmosphere at 37°C for 24 h in an incubator.
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PMC11755519
Proteins of the TWIST family are basic helix–loop–helix transcription factors.
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PMC9429973
Aims: This study aimed to compare the clinical outcome of severe to critical COVID-19 patients with CKD on RRT who received COVID-19 CPT versus those who did not.
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PMC11389534
CDK5RAP3 interacts with UFL1, thereby stabilising itself and facilitating its relocation to the ER in conjugation with the E3 complex. ,
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PMC11724582
CAFs are major sources of cytokines and chemokines.
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PMC11451940
Each of the complexes revealed one of the dominant peaks in the positive ionisation mass spectra at m/z = 636.33 (1); 498.25 (2); 460.23 (3); 537.35 (4); 637.36 (5); 499.29 (6); and 485.27 (7) corresponding to the [M(Lap)(N–N)] ion, followed by the peaks belonging to the [M(N-N)2] ions and usually less intensive [M(Lap)(N–N)2] ions.
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PMC10502403
Adoptive cell therapy using allogeneic γδ-T cells is a promising option for off-the-shelf T cell products with a low risk of graft-versus-host disease (GVHD).
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PMC9429973
ADAs were persistent in 5/6 pts (83%).
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PMC8973725
Furthermore, Western blotting indicated that ectopic expression of ZNF677 downregulated and upregulated vimentin and E-cadherin expression, respectively, in 786–0 cells (Figure 5b).
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PMC11472569
Corcoran et al. demonstrated that exosomes derived from docetaxel-resistant prostate cancer cells notably enhanced docetaxel resistance in previously sensitive recipient cells, partly attributed to the transfer of ABCB1.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", ...
PMC4360730
The ‘Organ’ facet represents the biosample slim described in the text describes the anatomical structure.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "The", "‘", "Organ", "’", "facet", "represen...
PMC6901583
Renewal of the medium was performed every third day untill the 14th day, after which cells were fixed and stained with crystal violet.
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PMC10728200
The membrane was then washed and visualized using enhanced chemiluminescence (ECL) solution (Millipore, Beverly, USA) and analyzed using ImageJ software (Version 1.53, National Institutes of Health, Bethesda, MD).
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PMC8316344
Cells were stained with C11-BODIPY (D3861, 1:1000, Invitrogen) or Sytox green (KGA261, 1:1000, KeyGEN BioTECH, Nanjing, Jiangsu Province, China) and fluorescence-activated cell measuring was performed with BD Canto ll.
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PMC11732628
According to the STRING database, NUSAP1 binds to CDCA8; (B) western blot was used to detect the protein expression of the CDCA8 and ATF3. **,
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PMC11093197
Pull‐down assay of RAS protein and GTP protein.
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PMC11597291
The blank (non-loaded with PCT) nCGO-PEG particles had lower cytotoxicity at all concentrations and incubation times than the blank nCGO particles (Figure 5), indicating an increased biocompatibility of the pegylated graphene oxide particles.
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PMC11267830
Additionally, Western blot analysis revealed the absence of THOC1 signals following RBM39 depletion (Fig. 7b).
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PMC11792740
It also highlights some of the key therapeutic strategies to counteract T-cell depletion, including chimeric antigen receptor T (CAR-T) cell therapy, tumor vaccines, and combination therapies, all of which are critical for cancer immunotherapy Common methods used to evaluate T-cell depletion and key therapeutic strategies to overcome T-cell depletion.
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PMC11787355
Created in BioRender.
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PMC11474209
Improving and compressing these biosensors to be integrated into portable, field-deployable devices is one of the most promising future directions.
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PMC9429973
K. Nachtkamp, C. Zahner, A. Fricke, T. Ulrych, F. I. Schulz, A. Kündgen, P. S. Jäger, G. Kobbe, U. Germing Klinik für Hämatologie, Onkologie und Klinische Immunologie, Uniklinik Düsseldorf, Düsseldorf, Germany Background: Advanced age is strongly associated with inferior overall survival in patients with acute myeloid leukemia (AML).
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PMC10243817
This finding was further confirmed by immunofluorescence staining study showing reduced numbers of cells with p-SMAD2 nuclei in siTGFBR3 transfected and submerged cultured HNECs after stimulation with TGF-β2, but not TGF-β1 (Figure 5E).
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", ...
PMC11387401
NC nucleocapsid, MA matrix, IN integrase, PR protease, CA capsid. (
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PMC9100622
The intracellular ROS level was studied using a Probes CellROX Deep Red kit (C10422; ThermoFisher Scientific) and lipid peroxidation using Bodipy 580/591 C11 (Lipid peroxidation sensor) (D3861; ThermoFisher Scientific).
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PMC11612315
Overlap of FITC-exendin 9–39 (green), anti-human TRPV1 antibody (red), and Hoechst (blue) in the merged image.
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PMC9878562
To identify such chemotactic receptor, we performed RNA-seq analyses of the transcriptomes of VAL-wt and VAL-ko.
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PMC11763111
G Histogram plots show the percentage of IL10, IL4 and CCR2 expression in CAR-T cells from peripheral blood using flow cytometry.
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PMC11594074
Three independent repetitions of the experiment were performed.
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PMC9444294
Synovial sarcoma is an aggressive malignancy that generally affects adolescents and young adults and is characterized by high rates of recurrence and metastasis, with a 10-year survival rate of about 50%.
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PMC11549612
BOLA proteins have been found to modulate cell cycle progression by interacting with cyclins, cyclin-dependent kinases (CDKs), and other cell cycle regulators.
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PMC11757131
Data are shown as mean ± SEM.
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PMC11761919
Table 1GroupsNumber of chickensImmune dosage of microcapsulesChallenge with FAdV-4/GS01Negative control25PBS (pH 7.2)PBS (pH 7.2)Micro300.1 g10 ELD50E.
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PMC7351993
3Various types of cells imaged by the RIACS.Processing of the raw images was performed using ImageJ. Scale bars, 10 µm.
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PMC10142392
Furthermore, this work will emphasize the importance of the type of architecture, chain topology, self-assembly parameters, and comparison between multi-arm structures and linear counterparts in relation to their impact on their performance as nanocarriers.
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PMC11680578
Bar 5 mm. (
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PMC11644761
In differentiated cells induced to re-enter the cell cycle with forskolin, a significant increase in cyclin CCND1 expression was observed with 100 μM pterostilbene treatment at both 4 and 24 h. Conversely, at the lower concentration (10 μM), a significant increase in cyclin CCND1 expression was observed only after 24 h of pterostilbene treatment (Figure 1C).
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PMC11653533
Methanolic extract of F. benjamina stem, root, and leaves exhibited antioxidant potency with IC50 values of 50.1, 58.81, and 49.86 μg/ml, respectively (Imran et al., 2014).
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PMC11732628
MiR-490-3p overexpression facilitates the cell apoptotic level and cycle arrest in osteosarcoma. (
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PMC11473843
Prior to RNA sequencing, A549 cells were treated overnight with vehicle (DMSO), XMD8-92 (10 µM), Seliciclib (10 µM), alone or in combination (Fig. 4) or were treated either acutely with VS-4718 (12 h) or rendered resistant by treating them with increasing doses of VS-4718 overtime to select for drug-tolerant subpopulations (VS4718-T) (Fig. 5).
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PMC11240448
The strengths of causal connections inferred by BMRA depend on the time point at which data have been collected, because the network evolves over time .
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PMC10993311
Women who lost or gained little weight during pregnancy according to IOM (Rasmussen and Yaktine, 2009), who had a stillborn child or congenital diseases, or who did not attend one of the appointments during follow-up of the study were also excluded.
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PMC10993311
T3 = 30-36 weeks and T4 = delivery.
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PMC11777207
The Cd80 gene was amplified from a mouse CD80/B7-1 gene ORF cDNA clone in a pGEM-T plasmid vector (Sino Biological) using primers containing restriction sites at the 5′ end with Xho1 sequence and EcoRI sequence for the full-length CD80 primers.
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PMC9681981
Statistical model and data analysis The experiments were carried out in a completely randomized design with a 3 × 2 factorial arrangement (6 treatments and 3 replications).
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PMC7987994
We present the variance of the results also for the control.
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