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PMC10587429
We found the mice tolerated well with this dose, and magrolimab treatment significantly slowed down tumor growth compared to the control IgG treated condition ( Figure 4B ).
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PMC9918897
There were no statistically significant differences in tumor size between the groups.
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PMC6617630
This study demonstrates that, apparently, there is no distinctive feature of DHL/THL lymphoma with respect to sensitivity to BET inhibitors, and MYC-expressing lymphoma cells are probably addicted to the MYC-oncogenic effect and rely on MYC for their growth regardless of MYC rearrangements.
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PMC11684297
The SEM images (Fig. 1f) of as-synthesized ZnO-NFs exhibited peony flower-like morphology with the protruded petals visible in the nanometer range.
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PMC6160470
Also, no difference in its expression was observed when immunofluorescence was monitored with P-gp antibody that recognizes the inner epitope of the transporter (Supplementary Fig. S13B).
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PMC9429973
All patients were included in the PETHEMA registry (NCT02606825).
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PMC3681794
Drug resistance was not induced by HIF-1α stabilisation in normoxia by cobalt chloride nor reversed by the suppression of HIF-1α in hypoxia by shRNAi, siRNA, dominant negative HIF or inhibition with the small molecule NSC-134754, strongly suggesting that hypoxia-induced drug resistance in osteosarcoma cells is independ...
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PMC11269933
For western blotting analysis, gel was washed using wash buffer [25 mM Tris, 192 mM glycine, 0.1% (v/v) SDS, 10 mM EDTA] for 10 min three times to remove metal ions, followed by one wash without EDTA for 10 min.
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PMC10297204
The results of the MTT assay showed that cytotoxicity appeared stronger when the ethanol concentration exceeded 30%, which was comparable to the 99.5% ethanol concentration.
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PMC11334037
The concentration of lactate in transfected cells was determined by ELISA with lactate assay kits (MAK064; Sigma-Aldrich, St Louis, MO, United States) according to the manufacturer’s protocol.
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PMC11713588
FTD is the most common dementia in people under 60.
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PMC11521786
The experimental scheme followed: athymic mice were challenged subcutaneously with 3 * 10 cells of DU145 tumor cell line (diluted in 100 µL of PBS and injected into the right flank).
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PMC11765678
Furthermore, ER also binds to similar binding sites as Adriamycin in P-gp (Figure 7) and TPT in BCRP (Figure 8).
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PMC11718809
Compared to cow’s milk, yak milk contains higher levels of protein, fatty acids, antioxidant vitamins, and other beneficial components , classifying it as high-quality milk.
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PMC5941560
Finally, if present, CNVnator calls that overlapped the GATK/Pindel/BreakDancer set were removed, and the non-overlapping calls were merged with the variant calls from the other tools.
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PMC11703992
Staining solutions were added to each sample, and analyzed by flow cytometry immediately after a 10–15 min incubation.
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PMC11469524
Side-by-side comparison of the model coefficient (path)s (log scale) with fixed inhibitor strengths set to mean of both cell line models.
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PMC11721295
KRAS is commonly mutated in adenocarcinomas.
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PMC11172027
Conclusion: NASeLM could clearly increase caspase-3 activity in both cell types, Jurkat or MTC-SK cells, and thus induce cell death.
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PMC9368503
These data demonstrate that there are distinct biological pathways operating in ICR-SS-1 versus the SYO-1 and HS-SY-II cell lines.
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PMC11365983
These data have been deposited in NCBI's Gene Expression Omnibus (Edgar et al. 2002) and are accessible through GEO Series accession number GSE227703 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE227703).
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PMC11098378
Our Y2H screens with IpaH4 bait identified 12 human proteins as putative IpaH4 interactors with >2 independent clones identified across two screens (Figs 5D and S4A, and S3 Table).
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PMC9118379
Our results reveal that variant peptide identification that only passes the FDR threshold is insufficient for ensuring authenticity, and these examinations can reveal low-confidence variant events.
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PMC11021472
Statistically significant changes between the shRNAs (sh1 + sh2) compared to the control shRNA were determined by a moderated t-test using Benjamini–Hochberg multiple testing correction combining both experiments.
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PMC11357960
SUMO1 predicted the probability of NR with a sensitivity of 75.0% and specificity of 67.9% (cut-off value: 1.5; area under the curve [AUC]: 0.715; 95% confidence interval [CI]: 0.614 to 0.815; P <0.001).
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PMC8303372
The results showed homogenous spherical NPs with a diameter of approximately 280 nm and a zeta potential of ~+20 mV. The release experiments highlighted the possibility to control the amount of bioavailable GA from NPs, succeeding in a prolonged retention profile of the drug.
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PMC11713609
Work model demonstrates the involvement of PHLDA2 in p53-mediated tumor suppression through modulating ferroptosis.
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PMC3813807
Nevertheless, the stomach is a mixture of different cell types and detection of miR-373 and TNFAIP1 by other methods may be required.
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PMC10976516
Treatment led to overall longer survival and tumor regression.
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PMC11185260
However, scL-vec treatment did not affect tumor growth.
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PMC11592837
Furthermore, we anticipated a delayed cytotoxicity of PBMCs in response to DOX beyond 8 h of incubation, which was not covered by our experimental design.
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PMC11186948
Remarkably, RNA-seq performed on #249, HD and RSTS cells shows differentially expressed genes (DEGs) common to #249 and RSTS.
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PMC8427838
A EV/786-0 and TMIGD1/786-0 cells were stained with an antibody to K40 acetyl-α-tubulin (green) and phalloidin/actin (red).
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PMC11732523
Recent evidence suggests that patients with primary immunodeficiency disorders (PIDs) may not face a higher risk of death compared to the general population.
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PMC11699465
CUT&Tag peak distribution created using deep Tools computeMatrix and plotHeatmap.
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PMC9635307
Flow cytometry revealed high expression of CEACAM1 on B cells in secondary lymphoid tissues.
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PMC6182045
Finally, the strategy used to produce a TZ97008 gp120 in the MGAT CHO cell line can be applied to the production of other candidate HIV vaccines.
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PMC9429973
In addition, 61 pts presented with a myeloid malignancy (46 with MDS, AML, or CML, and 15 with a MPN), 42 presented with a lymphoid malignancy (ALL, CLL, or lymphoma), 8 presented with bone marrow failure, and 5 presented with other HD.
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PMC11791478
After a 15-minute incubation at 37°C, cells were treated with FVIIa (final concentration 20 nmol/L), followed by an additional 5-minute incubation at 37°C.
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PMC11089031
To determine the prognostic value of ZNF692 expression in ccRCC, the web tool Gene Expression Profiling Interactive Analysis (GEPIA, https:// http://gepia.cancer-pku.cn/detail.php) was used to visualize the correlation between ZNF692 expression and the overall survival (OS) of ccRCC patients, with the median expression...
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PMC7229095
The clones with mini-pool assessment or ClonePix2 experiments were screened by evaluating the productivity using an Octet QKe system (ForteBio, Fremont, CA, USA).
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PMC11746948
h Heatmap of cell viability in A375 cells pretreated with 2-BP for 24 h, followed by 24 h RSL3 (1 μM) treatment with various inhibitors, including z-VAD-fmk (20 μM), necrostatin-1 (2 μM), Bafilomycin A1 (10 nM), and Fer-1 (1 μM).
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PMC11075223
The content of caffeic acid, ferulic acid (FA), isoferulic acid (IFA) in stir-frying and wine-processed C. dahurica was significantly different from that in raw by ultra-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS).
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PMC8759873
The VBC complex participates in the degradation of various proteins in the human body and belongs to the E3-ubiquitin protease system.
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PMC7081114
To explore the role of KIF21B in hepatocellular carcinoma and its clinical significance.
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PMC9429973
Recently, LRPAP1 autoantibodies were detected in 13% of patients from the European MCL Network trials and were associated with superior outcome, while the autoreactivity of IGHV 4-34 antibodies is largely known.
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PMC9581083
S. divaricata, a member of the Umbelliferae family, is a perennial herb native to northern Asia.
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PMC11687167
Activation of the TLR signaling pathway is generally accompanied by the activation of NF-κB, which in turn facilitates the release of proinflammatory cytokines that trigger leukocyte aggregation and inflammation .
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PMC3035438
However, one limitation was that DT40 cells can produce only chicken IgM, in either its membrane-bound or secreted form.
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PMC10654497
Construction and validation of risk scores. (
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PMC9000591
In addition, SE1 showed weak antioxidant abilities, as presented in Figure 1.
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PMC10094712
In the case of selenoproteins, this task is complicated by the fact that the amino acid selenocysteine (Sec), which is part of them, is encoded by one of the three stop codons of translation.
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PMC10761218
Cell viability and in vitro cytotoxicity were evaluated using an MTT assay [21–23].
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PMC11337594
A representative density plot of Annexin V-FITC/PI staining is shown in Fig. 6B. (
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PMC11670407
Statistical analyses were conducted utilizing GraphPad Prism 9.0 software (GraphPad Software, USA).
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PMC11746948
We also observed that the degradation of GPX4-C66S could be inhibited by the proteasome inhibitor MG132 (Fig. 3o).
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PMC11739484
Accounting for the majority of skin cancer-related deaths, its incidence continues to rise globally, posing a significant burden on public health systems worldwide.
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PMC11337594
This was evidenced by the migration of the cell population toward the PI-positive and Annexin V-negative group after TER treatment in the CASP3-KO cell line.
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PMC11629143
ChIP‐qPCR demonstrated significant enrichment of p300 (1.4‐fold, p < 0.05) and H3K27ac (2.1‐fold, p < 0.05) at the CMV promoter in cells expressing dCas9‐p300, relative to those expressing unfused dCas9.
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PMC11400680
Furthermore, it also incorporates an innovative concept called Generalized Efficient Layer Aggregation Network (GELAN), which enhances the model’s speed and efficiency while preserving accuracy.
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PMC11200978
Per manufacturer’s recommendations, sample concentrations were under 200 cells/µL (range: 75–200), and 5000 events per sample were acquired using a flow rate of 0.59 µL/s.
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PMC11612794
The authors are very grateful to Y. Kubota and R. Fukuoka for their technical assistance.
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PMC11344246
Separately, protein structures were downloaded from SWISS-MODEL (32,33) and the Protein Data Bank (34) (June 2019).
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PMC11674834
High levels of 3,5-DMA- and 3-EA- hemoglobin adducts were detected in nonsmokers, suggesting the importance of other sources for alkylaniline exposure, such as dyes, motor fuels, pesticides, and drugs.
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PMC11522251
Using a Multi-function Enzyme Labeler iD3 (MD, USA), the absorbance of OD 490 nm was determined.
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PMC9429973
Using of complexes, containing microRNAs with anti-tumor activity, leads to induction of apoptosis of leukemia cells.
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PMC7802142
c Western blotting and d quantitative analyses of C-myc and Cyclin D1 in cells were performed 48 h after treatment.
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PMC9596868
MST-312 displays two different types of effects on cancer cells.
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PMC10706275
For the detection of the HSA gene expression in cell lines with SSI, the Western blot analysis was conducted as follows: 20 µL of denatured cell culture supernatant was loaded onto a 10% SDS-PAGE gel.
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PMC11594806
For localized and metastatic soft tissue sarcomas, DOX and IFO are considered first-line options .
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PMC9429973
Image: Summary/Conclusion: In our real-world cohort of patients with AML, agreement between derived response per 2017 ELN criteria and physician-assessed response was 65.1%.
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PMC9386809
Splitting patterns are described as singlet (s), doublet (d), triplet (t), multiplet (m), and broad singlet (br s).
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PMC9429973
Gene-expression profiling studies led to the proposal of two main cell-of-origin molecular subtypes known as germinal center B-cell-like (GCB) DLBCLs, and activated B-cell like (ABC) DLBCLs.
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PMC11216402
For all other samples, the ATAC-seq- and RNA-seq-based cluster labels are consistent.
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PMC9424261
The ubiquitination of GSS increased after erastin and RSL3 were added (Fig. 5C).
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PMC11803149
Cells were collected, washed, and resuspended in P4 primary cell nucleofector solution.
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PMC7334607
This is because genotypic changes that cause increased fitness in the presence of the first drug also allow increased fitness in the presence of the second drug as well.
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PMC11359735
Here, we use metabolic labeling and an ultra-sensitive myo-inositol detection methodology to provide evidence that CSP is indeed modified by a GPI anchor.
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PMC11033180
Upon neuronal depolarization, pretreatment of enteric neurons with Sema5A reduced the phosphorylation at Ser603 and Ser9 compared to neurons pretreated with Fc.
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PMC11502327
The experimental results showed that the fiber material could inhibit the proliferation and adhesion of SKOV-3 cells and control the release of DOX, which was also found in a kind of magnetic ferric oxide nanoparticles coated with chitosan (196).
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PMC11026382
b Heatmaps of the growth rate effect for all DHFR single mutations.
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PMC11143482
The majority of affected bones are the distal femur (30%), proximal tibia (15%), and proximal humerus (15%) due to the presence of developing growth plates .
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PMC8701144
Analysis of the effect of the E1B-55k protein on WNT signaling was performed using the HEK293-STF/Ren cell line where the β-catenin responsive TCF/LEF driven reporter (7xTCF/LEF binding sites regulating Firefly luciferase expression) and the constitutive CMV Renilla luciferase constructs were stably integrated .
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PMC9412887
Gu et al. constructed a PD173074 (a kind of FGFR1 inhibitor) and chloroquine (CQ) dual-loaded shell–core targeted delivery system (CP@NP-cRGD), which could observably inhibit FGFR1 and autophagy, respectively, to reverse AZD9291 resistance (Figure 6a).
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PMC9429973
Bone morrow aspiration at day 15 was performed for all our patients.
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PMC11271278
Our analysis showed that, in addition to the copy number amplification, hijacking MYC enhancers on the same ecDNA may also contribute to ERBB2 and its surrounding oncogenes’ overexpression.
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PMC11486946
d Timelapse single-molecule images acquired at 0 and 80 min after the compound treatment.
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PMC11093197
In MCF10A cells, we did not observe the upregulation of DNA damage response‐related genes, including p21 following RB1 depletion.
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PMC9429973
Among them, 48 cases were stained for MYC in immunohistochemistry with 16 tFL.
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PMC11155445
XRD analysis of novel compound (Z)-4-bromo-N-(4-butyl-3-(quinolin-3-yl)thiazol-2(3H)-ylidene)benzamide (13b) showed that the compound crystallized in triclinic crystal system adopting P1̄ space group.
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PMC6835428
Using the HA–CH–siPLXDC1 particles (150 mg/kg, IV twice per week), the authors showed a significant decrease in the level of PLXDC1 mRNA in the tumor mass compared to CH–control siRNA and CH–siPLXDC1.
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PMC11612508
Treatment options are limited due to tumor heterogeneity, glial stem cell development, invasion, apoptosis, and resistance to radiation and chemotherapy.
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PMC11583010
SUM190R was produced by serial passage of the tumor cell line in mice chronically treated with an EV-like ADC (15).
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PMC11757131
The results of the EdU detection method were consistent with those of CCK8 method; the group treated with 0.8 mM 3-HIB demonstrated higher cell proliferation (Figure 1).
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PMC9429973
A particular sulfonamide drug, indisulam, was previously studied in cancer clinical trials where it was found to be safe but demonstrated limited efficacy, most likely because neither the mechanism of action nor potential biomarkers of response were known.
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PMC11653533
Fahmida Tasnim Richi: Writing – original draft, Visualization, Project administration, Methodology, Investigation, Data curation, Conceptualization.
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PMC11694625
H NMR (500 MHz, DMSO-d6) δ 11.35 (s, 2H, NH), 9.38 (s, 2H, CHN), 8.96 (dd, J = 4.1, 1.7 Hz, 2H, CHquinoline), 8.44–8.41 (dd, J = 4.0, 1.7 Hz, 2H, CHquinoline), 8.41 (dd, J = 3.9, 1.5 Hz, 2H, CHquinoline), 8.03 (dd, J = 8.1, 1.2 Hz, 2H, CHquinoline), 7.75 (t, J = 7.7 Hz, 2H, CHquinoline), 7.60 (dd, J = 8.3, 4.1 Hz, 2H, ...
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PMC11045125
Immunohistochemical analysis of the (rare) ΔEBNA2 + Myc lymphomas found to express both high level Myc and LMP1 on immunoblot analysis revealed that tumors are composed of geographically distinct foci containing either high level expression of Myc and Myc-induced target genes (including CD10, TDT, TCL1, and CD179B) alo...
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PMC11739484
Through the proteolytic processing of growth factors, cytokines, and cell surface receptors, MMPs exert pleiotropic effects on tumor cells and their microenvironment, creating a permissive niche for tumor growth and dissemination.
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PMC11791432
At the same time, ANXA1 can promote actin cytoskeleton rearrangement, cell polarization, and cell migration .
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