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PMC7334607
The right panel of Figure 4 demonstrates how samples were ranked based on their response to vorinostat (SAHA) and SP-2509 (SP).
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PMC3203927
Terminal differentiation is preceded by inhibition of proliferation and cell cycle arrest.
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PMC9429973
Given the high unmet need in this R/R pt population, these initial data are encouraging; further clinical evaluation is warranted.
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PMC11209164
After an additional 24 h incubation, visible plaques were fixed with a mixture of methanol and glacial acetic acid in a 3:1 ratio for a minimum of 1 h, and then stained for 30 min with a Coomassie Blue solution (comprising 4 g of Coomassie Brilliant Blue R (Sigma, Oakville, ON, Canada) (cat.
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PMC9429973
V. Goyal, A. Singh, Y. Memon, N. Thakre, P. Sahu, R. Goyal Sanjeevani CBCC Cancer Hospital, RAIPUR, India Background: Since December 2019, COVID-19 has aroused global attention.
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PMC9429973
Calr bone marrow (BM) was differentiated ex vivo, subsequently enriched for megakaryocytes, and subjected to quantitative proteomic analysis.
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PMC9429973
All patients provided informed consent for the prospective collection of their data.
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PMC10545062
RESUSCITATION #SHOCKThe treatment of severe circulatory shock often requires intensive care unit (ICU) admission and invasive monitoring devices.
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PMC11597291
Hussien et al. investigated the anticancer effect of superparamagnetic GO (MGO) formulations conjugated with MUC1 targeting aptamer and further loaded with PCT.
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PMC11295144
Purified GST fusion proteins were detected by Ponceau S (lower panel). (
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PMC11621493
The plots display the GSEA statistics, including the “normalized enrichment score (NES)” and FDR-adjusted P value. (
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PMC11787381
The average hydrodynamic diameter associated with the polydispersity index (PDI) of the liposomes were measured by dynamic light scattering (DLS) using a Zetasizer Ultra (Malvern Panalytical, Worcestershire, UK), equipped with a 633 nm laser at a fixed scattering angle of 173°.
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PMC11486946
The lysate was electrophoresed on a 10% SDS-polyacrylamide precast gel (192-14961, SuperSep Ace, 10%, 17well, FUJIFILM Wako Pure Chemical, Japan) Then, the proteins were transferred to a 0.45 μm pore PVDF membrane (034-25663, ClearTrans PVDF Membrane, Hydrophobic, 0.45 µm, FUJIFILM Wako Pure Chemical, Japan) and reacte...
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PMC11747467
The expression distribution of GATA Binding Protein 3 (GATA3) and Forkhead Box A1 (FOXA1) was used to identify the zone where luminal cells are located, while CD44 Molecule (IN Blood Group) (CD44) and Keratin 5 (KRT5) were used to determine the zone where basal cells are probably located.
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PMC11489175
In fact, the expression of USP28 has been highly correlated with the CTNNB1 gene that codes for β-catenin as well as CCND1 and BIRC5, both targets of the Wnt/β-catenin signaling pathway.
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PMC10976516
The most researched GM-CSF expressing OV is T-VEC.
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PMC9429973
At progression, all patients harbored at least 1 mutation by TDS, except one patient in which only 2 mutations in BCR-ABL1 KD were detected.
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PMC7081114
Stable knockdown cells were selected using puromycin.
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PMC11700247
By facilitating the targeting and elimination of tumor cells that have lost the CAR T-cell target antigen, endogenous TAA-specific T cells address issues related to tumor heterogeneity and metastatic spread.
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PMC11599565
We called this phenomenon “Epithelial-Mesenchymal-Ectodermal Transition” (EMET).
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PMC9429973
CD70 is a co-stimulatory protein with temporally limited expression on activated lymphocytes and is highly expressed in many TCLs.
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PMC11695623
Previous studies have shown that these biological processes are closely related to the occurrence and development of tumors.
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PMC11791264
Left panels provide a graphical representation of the accumulated number of migrated cells at 48 h, and the right panels show the representative images (200 × magnification).
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PMC10568263
However, drawbacks of high cost, complicated, time-consuming, and invasive diagnosis limit their potential for point-of-care testing (POCT).
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PMC11713679
Additionally, it will assess the therapeutic potential of a of bilobalide a biologically active terpenoid extracted from Ginkgo biloba.
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PMC8753818
The result of this study revealed the dominancy of caspase-3 in apoptosis, because in the absence of caspase-7 the enzymatic activity of caspase-3 increased which led to apoptosis progression and cell death.
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PMC9031474
Despite their high potential as novel anticancer agents, the mode of action and structure–activity relationships of drimanes and coloratanes have not been completely enlightened nor systematically reviewed.
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PMC11512230
Here, we have shown for the first time that AltMV SPV as well as TMV SP can adsorb on and penetrate Ewing sarcoma cells, opening up the possibility of using them as antitumor drug delivery systems.
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PMC11643361
This has driven the search for more selective therapeutic approaches that exploit the unique characteristics of cancer cells, including their altered metabolism, specific surface markers, and dysregulated signaling pathways .
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PMC6892818
Results are presented as Cell death fold increase in background-corrected RLU readings (mCD40L relative to control) and are representative of three independent experiments.
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PMC10142392
This finding suggests that the type of architecture, including variations in the number of branches, can affect the degradation process .
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PMC11746948
When the sequence cluster extends the complementary chain, each dNTP labeled by fluorescence can release the corresponding fluorescence.
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PMC11715644
Cells were cultured at 90–95% confluency, every three to four days.
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PMC11216402
To associate these expression differences with biological processes, we performed gene set enrichment analysis using PCA gene weights and gene set enrichment analysis (GSEA) (Mootha et al. 2003; Subramanian et al. 2005; Liberzon et al. 2011, 2015).
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PMC11588085
Our RNA sequencing data demonstrated an increased expression of CD24 in sgCtl-hypoxia compared to sgCtl-normoxia for OVCAR3-KRAB (Fig. 6A).
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PMC6222635
Therefore, we speculated that a mannofuranose was present in FWDOP1.
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PMC11519077
Till now, some promising clinical trials using MSCs have been done on IBD patients .
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PMC9429973
Within the TME, regulatory T cells (Tregs) represent a subtype of CD4+ T cells with immunosuppressive abilities, causing the evasion of cancer cells from the immune system.
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PMC11394386
This differential response may indicate that in SiHa cells, GSK3β inactivation could enhance mTORC1 activation and other transcriptional effectors, maintaining a positive feedback loop between pathways .
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PMC8567602
The distribution of tumors in these two groups differed significantly (Fig. 1B).
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PMC10887351
In NB mouse models, both non-selective and selective β1/β2-AR blockers increased the chemotherapy response and exerted antitumor activity .
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PMC7802142
a H&E staining of the skin from mice receiving depilation and 6-day subcutaneous injection (once per day).
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PMC9429973
The mean (SE) reduction in units of RBCs transfused following eculizumab treatment was reported in six studies and ranged from 1.5 (1.1; n = 6) to 14.3 (1.4; n = 75).
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PMC11719944
c-HDL samples were treated with enzymes modified with polyethylene glycol and dextran sulfate and analyzed using the same photocolorimetric technique.
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PMC11317131
U2OS cells were electroporated using the Neon Transfection system (Invitrogen).
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PMC10606998
The Williamson–Hall approach differentiates between the size-induced and strain-induced peak broadenings by considering the peak width as a function of angle .
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PMC9429973
No deaths related to TPO-RA was reported.
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PMC11487720
Further, UV/cold shock-treated cells were imaged by time-lapse holographic microscopy (HoloMonitor M4, Phase Holographic Imaging PHI Inc., Boston, MA, USA) and analyzed by Hstudio M4 Tracking software.
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PMC5673060
We have performed an analysis of the pTyr phosphoproteome of these cells using phosphopeptide immunoprecipitation of the SILAC labelled cell lysates in a previously reported study .
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PMC8345486
Dead cells were “trapped” in the spheroids, indicating that compound 23 acted inside the structure.
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PMC9429973
I. De La Iglesia San Sebastián, D. Carbonell, A. Pérez Corral, M. Bastos, R. Bailén, M. Silvia, M. Chicano, P. Muñíz, G. Oarbeascoa, J. Anguita, M. Kwon, J. L. Díez-Martín, I. Buño, C. Martínez-Laperche Department of Hematology, Hospital General Universitario Gregorio Marañón; Instituto de Investigación Sanitaria Grego...
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PMC11790971
f SLAM composite images of randomly selected CHO cells from different cell lines in passage 2.
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PMC9429973
We confirmed the NUP98::NSD1 fusion gene in 1.5% adults AML patients.
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PMC11143482
As shown in Fig. 3, food intake in all mouse groups was measured starting on Day 5 upon tumour cells inoculation.
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PMC11607321
c–e, similar to b, using MOFA, MOVE and mean imputed values, respectively.
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PMC10040136
Both conditioned medium and recombinant TGF-β2 protein activate the PI3K-AKT signaling pathway of GIST, and the TGF-β2 monoclonal antibody antagonizes these effects (A, B).
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PMC9927933
Collectively, these results clearly showed that in in vitro cell culture models, UBE2C is essential for growth and survival of lung cancer cells harboring a mutant Kras.
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PMC9429973
The frequencies of T and B cells positively correlated between the PB and LN compartments (r=0.3855, p=0.0243, and r=0.5203, p=0.0016, respectively).
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PMC11461874
The half-life of protein was calculated using exponential regression and shown on the right as mean ± SEM.
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PMC11536589
Data were expressed as mean ± SEM.
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PMC6600592
m.p.
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PMC11629192
One week after IA, seizures recurred, accompanied by rhabdomyolysis and intermittent heart rate dropped (20–60 bpm).
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PMC9895440
All cells were confirmed to be negative for mycoplasma by PCR as described after every freeze-thaw cycle and then passaged every 2–3 days for a maximum of 15 passages.
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PMC11216402
To assess if these TFs also drive variation in tumors, we next trained gkm-SVM on differential peaks in normal stomach versus primary stomach tumor samples (The Cancer Genome Atlas for Stomach Adenocarcinoma [TCGA-STAD]) (Fig. 1C; Supplemental Figs. S4, S5).
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PMC9429973
The primary endpoint was overall response rate (CR+PR+mCR).
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PMC11026382
Dotted lines indicate multiple intermediate reactions summarized with a single line.
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PMC11763111
As cells divide, the fluorescence intensity decreases, generating a series of peaks in the flow cytometry histogram.
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PMC11435360
We also reported a decrease in CD4 cell infiltration after monotherapy with PAG and combination therapy with GEM.
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PMC9777812
The pure mammary epithelial cells were isolated after passages 3 to 4.
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PMC11774251
Initially, we screened 100 potential targets associated with the chemical structure of Tan-I utilizing the SwissTarget platform.
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PMC11787381
In order to link the in cellulo activities of bambuterol to its effects both towards BuChE and β2-AR, we undertook the measurement of the bambuterol-dependent cAMP production in COS-7 cells overexpressing β2-AR, using a CAMYEL cAMP sensor and bioluminescence resonance energy transfer (BRET).
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PMC11087766
N = 187 cells (mCherry only = 23, sfGFP only = 15, mCherry/sfGFP with colocalization = 148, mCherry/sfGFP without colocalization = 1). (
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", ...
PMC11045125
To determine if Myc over-expression in human CB B cells cooperates with ΔEBNA2 EBV infection to form B-cell lymphomas in NSG mice in vivo, and/or alters the phenotype of ΔEBNA2 EBV-infected lymphomas, ΔEBNA2 EBV-infected CB B cells were co-infected with a Myc-expressing retrovirus as described in the Methods.
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PMC5034105
Although the pathogenesis of ZIKV-associated neurological complications has been reported in recent studies, key questions on the pathogenesis of the other clinical manifestations, non-vector-borne transmission and potential animal reservoirs of ZIKV remain unanswered.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", ...
PMC4816271
An increasing body of clinical data of CD19 CAR in B-cell malignancies as well as a double-marking study suggest that CARs providing additional co-stimulatory signals result in improved persistence and efficacy.
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PMC11626627
p < .05, **p < .01, ***p < .001, ****p < .0001, ns, not significant compared to the controls, and Scale bar: 250 μm.
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PMC9681981
Then, the bacterial culture was centrifuged at 8000 ×g for 20 min at 4°C, and the bacterial deposit was rinsed twice with a cold phosphate-buffered saline (PBS) solution (pH=7.4).
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PMC11635087
The homogenized sample in a centrifuge tube was separated into two dark green layers after a homogeneity-gradient centrifugation: cell debris (upper layer) and isolated chloroplasts (lower layer). (
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PMC9429973
Cord blood (CB) is a convenient source of T cells with clear advantages.
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PMC9429973
Two patients discontinued ZR-CHOP regimen during treatment, one with R-CHOP in last two cycles due to drug rash and HBV reactivation.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Two", "patie...
PMC9429973
5 out of 10 clusters of PCs in AL patients were found to be associated with protein processing in the endoplasmic reticulum and apoptosis pathway.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tok...
PMC11638288
The expression of NKp46 is closely associated with the prognosis of certain diseases.
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PMC11806106
The agonist-induced activation was normalized to the maximal activation (mean ± SEM, n = 5 of independent transfections measured in triplets) and plotted as concentration-response curve for dynorphine A in (B) (EC50: 219 nM).
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PMC11589157
Table 3Chromosome sizes and assignment for Hi-C scaffolds.
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PMC11352877
An ordinary one-way ANOVA was used (Tukey’s multiple comparisons test).
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PMC9429973
Clotting factors dosage was normal except for FV: 0.10% with a FV inhibitor of 19.45 UB.
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PMC11576293
However, the H NMR spectra of intermediate 8 in DMSO-d 6 exhibited an unexpected pattern, showing that 8 is in the ureide-like 3,4-dihydropyrimidine-2(1H)-thione tautomeric form (c-form), as seen in Figure 3.
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PMC9919300
Against this background, the aim of the present paper was to investigate the activity of these phytochemicals in the experimental cellular model of NAFLD.
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PMC11225860
Cells were then released from the block by washing with fresh media, supplemented with 1 % final volume DMSO (B).Fig 3 Analysis cell cycle progression using FUCCI U2932 cells.
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PMC9622879
This result is another reason that the daily energy cost of the community with the proposed CEMS is smaller than that of the others.
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PMC11534035
Statistical analysis.
[ { "tags": [ "O", "O", "O" ], "tokens": [ "Statistical", "analysis", "." ] } ]
PMC9429973
Patients who subsequently tested positive for SARS-CoV-2 until 31 March 2021 were analyzed for morbidity, mortality, need for supportive care and risk factors involved in COVID-19.
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PMC10728200
The synthesis of GSH is contingent on the availability of intracellular cysteine, which can be generated from cystine imported from the extracellular space via the cysteine-glutamate antiporter (Systemxc-, composed of recombinant solute carrier family 7, member 11, SLC7A11, and recombinant solute carrier family 3, memb...
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PMC11612794
The ratio of M phase population in indicated cell lines.
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PMC7154001
Metabolism Studies (Metabolite Profiling) of Conventional Synthetic Anticancer Drugs Abbreviations: AMS, Accelerator Mass Spectrometry; ARC, Accurate Radioisotope Counting; DLM, Dog Liver Microsome; ESI, Electrospray Ionization; GC, Gas Chromatography; HLM, Human Liver Microsome; HPLC, High Performance Liquid Chromatog...
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", ...
PMC11705630
To select the stably transfected cells, puromycin was used at concentrations of 1-10 μg/mL in culture media.
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PMC9429973
Out of these four newly detected variants (2 deletions, 2 translocations) were validated by PCR.
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PMC10933092
Many studies have reported that methylation of mRNAs and lncRNAs regulates numerous processes affecting cancer cell function by enhancing RNA stability, increasing RNA nuclear accumulation, and promoting the decay of some RNAs (Jin et al., 2022).
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PMC11718031
Subsequently, the cells started to consume lactate when grown in all basal media except CD-CHO, IS CHO-CD G17.4, and IS CHO-CD G17.7.
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PMC11757131
In summary, the addition of 0.8 mM 3-HIB to porcine mammary gland epithelial cells promotes cell proliferation by enhancing lipid metabolism and the expression of cell proliferation-related proteins.
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