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OTAR3088
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PMC11583690
3 (A) The FTIR spectra, (B) thermogravimetric analysis, and (C) isothermal adsorption–desorption of MSN and BPMO.
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PMC11546117
Glucose was purchased from Thermo Fisher Scientific.
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PMC11731614
Cryo-EM.
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PMC11348300
Five different groups of nude mice were incubated with different tumor cell lines: (A) pancreatic adenocarcinoma cells AsPc1, (B) pancreatic adenocarcinoma cells BxPc3, (C) ovarian carcinoma cells OvCar5, (D) glioblastoma cells U87, and (E) squamous cell carcinoma FaDu.
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PMC11643361
In the last few years, substantial progress has been made in investigating the anticancer potential of various scorpionate complexes, revealing their significant cytotoxic effects across different cancer cell lines.
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PMC5810978
These cultures were incubated for 5 days and then frozen and thawed for three cycles.
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PMC11762280
p-MLKL phosphorylated MLKL.
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PMC5941560
In order to minimize CNVnator’s false positive homozygous deletion calls, we removed regions with a copy number of zero if they overlapped any variants that were identified by GATK.
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PMC6312945
RT-qPCR results showed that miR-101 level in BEL-7404 pSUPER.neo-miR-101 group and miR-345 expression level in BEL-7404 pSUPER.neo-miR-345 group were higher than those in BEL-7404 pSUPER.neo group (P<0.05); thus, miR-101 and miR-345 expression levels in BEL-7404 cells were successfully regulated.
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PMC9429973
Results: We retrospectively described the clinical, biological, and genomic characteristics of a series of 36 LCH patients with thyroid involvement in our centre between January 2001 and December 2021.
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PMC11627398
The significant pathways were identified based on the criteria of |logFC| < 0.5 and p.adjust < 0.05.
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PMC9429973
Frequencies of dominant T cell clones varied between patients (1.1-20.6% of CD8 T cells).
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PMC11621565
The images were analysed by densitometry to evaluate the relative amount of protein staining and the results were quantified in terms of optical density using ImageJ software.
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PMC11301242
ecDNA amplicons were significantly larger in size (median 4.4 Mbp) than other identified amplicon classes (Fig. 1E).Fig.
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PMC11539788
The cells were maintained at 37 °C with 5% CO2 and appropriate humidity.
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PMC11286266
Statistical analysis was performed by a two-sided unpaired Welch’s t-test and significance is indicated by asterisks (****p < 0.0001). (
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PMC11015054
The obtained results were calculated using the 2 method with GAPDH as the internal control.
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PMC11643491
Spherical and stretched PS particles were then added at the same concentration (0.8 µg/mL) to incubate with the cells overnight.
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PMC11502443
Given the emerging importance of GIP receptor targeted therapeutics in metabolic disease, understanding the role specific signalling pathways play in physiological processes is of significant interest.
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PMC11731614
In both “omic” datasets, neuron-keratinocyte pathways were enriched both under normal physiology and in PDN.
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PMC10135767
Recently, we found that shear stress firstly strengthened, then weakened, the tethering and rolling behaviors via integrin α4β7/MAdCAM-1 at a lower threshold of shear stress (also known as the “catch bond mechanism”).
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PMC8097906
The fluorescence microscopical images were captured on a confocal laser-scanning microscope Leica DMi8 (Leica, Wetzlar, Germany).
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PMC11357960
The 9th week is in line with the recommended time node for the first response assessment of tumor according to the guidelines and consensus (26–28).
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PMC4270159
d. Dilute HRP-secondary antibody in 5% milk and add to the blots.
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PMC10957991
In the mammary gland, TRPV4 levels have been shown to increase significantly at Day 15 of gestation and decline shortly after lactation.
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PMC11297139
Bottom right: quantification of the weight of the extracted tumors.
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PMC11478724
In the A549 cell line, LDH activity rose by 14.33% (P = 0.0002) after 24 h of treatment, and by 39% (P < 0.0001) after 48 h of treatment.
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PMC11528603
The bioactivities of S. affinis even extended toward anti-plasmodial trait as demonstrated by Abdul-Wahab Salae et al. It is reasonable that these bioactivities, especially anticancer features, might come from the contained phytochemical constituents of S. affinis.
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PMC11525028
Further mRNA stability assays demonstrated that ac4C site mutation significantly decreased the half-life of ATF4 mRNA (Figure 3S).
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PMC9429973
Although there is growing evidence supporting multidisciplinary molecular tumor-boards (MTB) in solid tumors, only a minor subset of MTB recommendations affects hematologic malignancies.
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PMC8633974
Then we dissolved them in 1500 μL of formulation buffer to produce the aqueous phase.
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PMC11625890
My research project was based on using a neuroblastoma cell line to investigate the overactivation of the protein AMBRA1, to initiate a shift away from apoptosis and towards autophagy/mitophagy, a potential strategy for treating neurodegenerative diseases such as Alzheimer's and Parkinson's diseases.
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PMC10502403
The median basal OCR/basal ECAR ratios were 0.92 and 2.33, respectively.
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PMC11761919
The levels of enzymes AST, ALT and LDH are indicative of liver function.
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PMC9429973
The research was supported by the Key R&D Program of Zhejiang, No. 2022C03137; Public Technology Application Research Program of Zhejiang, China, No. LGF21H080003; the Key Project of Jinhua Science and Technology Plan, China, No. 2020-3-011; the 2019–2024 Academician Workstation of the Fourth Affiliated Hospi-tal of th...
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PMC11244823
It is well documented for many herpesviruses that seropositivity may remain for life, although younger individuals are more likely to have experienced recent exposures, resulting in higher antibody reactions .
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PMC11400680
Based on the evaluation results on three different levels of EVICAN test sets, the proposed method demonstrates noticeable performances showing average mAP50 [mean average precision at intersection over union (IoU) =0.50] scores of 96.25, 95.05, and 94.18 for cell segmentation, and 68.04, 54.66, and 38.29 for nucleus s...
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PMC11506670
The wild-type NRAS and the canonical NRAS Q61K mutant constructs were previously cloned, and are available in the laboratory .
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PMC3995603
The animals were allowed to acclimatize for 2 weeks before the experiments were initiated.
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PMC11703896
are the drugs used against the receptors to supress the cancer (Desai et al. 2016).
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PMC6684588
A reporter-gene assay was used to evaluate the effects of ACE-083 on signaling by TGFβ superfamily ligands.
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PMC11519583
h MPs, which had been transfected with the specified siRNAs, were co-cultured indirectly with Cos7 cells that had been transfected with expression vectors encoding mWnt11 or mock vectors.
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PMC11461874
All animal experiments were approved by the Institutional Animal Care and Use Committee (IACUC) of the University of Tokyo.
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PMC11351758
In one of our recent studies, mROS formation was found to be potentiated in NARP cells upon the introduction of apoptotic insults such as laser irradiation-induced ROS stress and amyloid beta treatment .
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PMC8345486
The fluorescence intensity was determined using an LSRII BD cytometer with an excitation wavelength of 488 nm and emission in the green fluorescence channel FL1 and expressed as median fluorescence intensity (MFI).
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PMC11093197
Similar phenotypes were also discerned in HMEC but not in Trp53‐deficient MOSE cells (Figure S4A,B).
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PMC10968586
In the same cellular model, apoptosis seemed regulated by HT-mediated increase in the phosphorylated form of c-jun N-terminal kinase (JNK), which is a central player in establishing cell faith (apoptosis vs. proliferation) .
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PMC11578343
Photograph of the microfluidic platform, which consists of two independent microfluidic channels (blue boxes) embedded in PDMS and aligned to a glass substrate with prefabricated Pt electrodes and Au contact pads.
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PMC11451940
and zinc(ii) complexes with lapachol (HLap) of the composition [M(Lap)2(N–N)] and [Cu(Lap)(H2O)(terpy)]NO3 (4), where M = Cu (1–3) or Zn (for 5–7), and N–N stands for bathophenanthroline (1 and 5), 5-methyl-1,10-phenanthroline (2 and 6), 2,2′-bipyridine (3), 2,2′;6′,2′′-terpyridine (terpy, 4) and 1,10-phenanthroline (7...
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PMC11754094
f, The effect of ART558 (POLQ inhibitor) in reducing large-deletion events mediated by CGBE1 in HeLa cells measured by long-range amplicon sequencing (n = 3, mean ± s.e.m.).
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PMC11661024
The cell viability of two postbiotic derivates from L. fermentum CH and L. fermentum KH were calculated against cancer and normal cells using an MTT assay.
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PMC10589262
Xiang et al. were the first to report that pathogenic KIT signaling occurs from the Golgi.
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PMC4270159
The effect of the secondary kinase inhibitor alone will also be assessed.
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PMC11786767
However, concerns have arisen regarding the use of these iterative methods to visualize membranes or lipid-containing subcellular structures beacause of their use of strong lytic and denaturing reagents such as sodium dodecyl sulfate (SDS) and/or sodium hydroxide (37, 38, 39).
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PMC11271278
We performed unsupervised clustering of the cell lines based on a combination of the enhancer contact and interaction intensity values for each identified HAPI gene (Fig. 1C, see Methods for more details).
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PMC11588008
At present, there is no effective treatment method for POI induced by chemotherapy.
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PMC11609529
CA3 (0, 0.25, 0.5, and 1 μM) or VPF (0, 2.5, 5, and 10 μM) was added to the culture 6 h post-seeding.
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PMC11782780
BC is a common malignancy that primarily affects the epithelial tissue of mammary glands, significantly impacting the physical and mental health of women and even posing a threat to their lives.
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PMC11750696
Th1 cells notably produce proinflammatory cytokines such as interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) (38).
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PMC11755467
For the positive controls, 15 pmol of RNA with Enoxacin in DC buffer (50 mM HEPES pH 8.0, 4 mM EDTA) and 50% Formamide v/v were heated at 95 °C for 1 min, then 3 µL of 1M7 (from 100 mM stock) was added, followed by another 1 min incubation at 95 °C and snap cooling and incubation on ice for one minute.
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PMC8389560
Numerous cellular functions, determining both the normal physiology of cells and the processes of their death, including through apoptosis, activating both the early “commitment phase” through IP3R and the later “execution phase” .
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PMC11789597
C and D) Tumor weight and images were obtained from B‐NDG mice subcutaneously inoculated with MM1S cells expressing shNC or shCAV1 for 31 days.
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PMC9429973
This clinical trial is anticipated to start enrollment in May 2022.
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PMC11697189
A498,769-P, ORSC2, HK-2 and 786-O were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium (Gibco, CA, USA) supplemented with 10% FBS (FBS Premium, Pan Biotech, Germany) and 1% penicillin/streptomycin (Invitrogen, Carlsbad, CA, USA).
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PMC11535726
The phylogenetic analyses of the concatenated ITS, LSU and SSU sequences were conducted using Maximum Likelihood (ML) and Bayesian Inference (BI).
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PMC9429973
The morphological analysis also showed that ara-C and ola in monotherapy as well as in combination with ZnSO4 induced cell death mainly by apoptosis.
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PMC9429973
Subtype and expression of immune checkpoints of infusion products were detected.
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PMC9429973
Twelve doses of prophylactic IT chemotherapy with alternating cytarabine and methotrexate were administered.
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PMC11763111
F Survival curves of NSG ALL model (n = 5), the statistical significance was determined with log-rank test (Mantel-Cox).
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PMC6684588
P ≤ 0.05, ***P ≤ 0.001 vs. vehicle by one-way ANOVA with Dunnett’s adjustment. (
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PMC11451940
ROS induction in A2780 cells was investigated after incubation with complexes 1, 5, and 7 (at half-cytotoxic concentrations).
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PMC10926885
The capacity of cells to invade and migrate was then determined using the scratch test, and we discovered that NDE1 knockdown prevented the invasion and migration of T24 and 5637 cells (Figure 10E–H).
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PMC11747885
Recent studies claimed that knockout mice exhibited altered tRNA levels in the brain, suggesting a potential role for Rlig1 in tRNA biology.
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PMC11696576
Pretreatment with ROS scavengers (glutathione and N-acetyl cystine) rendered the cells completely resistant to cell death induced by AVX420 (Fig. 6d) in contrast to the addition of either AA or a mixture of nine eicosanoids, which did not improve cell viability in the presence of AVX420.
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PMC9429973
Z. Hanson, S. Kaur, S. Lee, M. Akhtari Graduate Medical Education; Internal Medicine; Hematology and Medical Oncology, Loma Linda Medical Center, Loma Linda, United States of America Background: Zinc is a necessary micronutrient used as a catalyst, structural element, and regulatory ion in many metabolic processes incl...
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PMC10565698
P < 0.05, ***: P < 0.001.
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PMC5963610
NGcGM3 ganglioside expression was identified with 14F7 mAb and it was located in cell membrane and cytoplasmatic cell.
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PMC6337547
The genus Salvia is a dominant genus of the Lamiaceae family, which is extensively distributed in China (84 species).
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PMC10993311
T2 = 20-26 weeks.
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PMC11772585
G MMP was measured by flow cytometry (n = 3).
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PMC11247842
First, we removed imputed variants with minor allele frequency (MAF) < 0.5% and imputation accuracy < 0.4, as assessed by the R given by the different imputation software programs.
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PMC7090062
After dehydration, samples were cut into sections (0.12 μm thickness) and stained with 0.2% lead citrate and 1% uranyl acetate.
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PMC9429973
Aims: We performed a case control study to determine the role of cystatin C as a predictor of subclinical cardiovascular dysfunction in SCD and b-thalassemia patients.
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PMC9429973
A lentivirus-transduction system was used for overexpressing genes, and siRNA transfection was applied to induce NFATC1 knockdown in a BTZ-resistant cell line ARH77.
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PMC8996378
Most of the active diterpenes were lipophilic compounds, thus corroborating previous studies that defined effective ABCB1 modulator candidates should have a log p value of 2.92 or higher (Wang et al., 2003).
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PMC11365427
This research aimed to identify potential target proteins to elucidate the direct molecular sensor of phenylalanine.
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PMC10812665
Several follow-up technologies were combined to better understand cellular changes triggered by the compounds.
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PMC9429973
Image: Summary/Conclusion: This study provides the first real world evidence on comparative effectiveness of ibrutinib, acalabrutinib, and zanubrutinib in MCL patients.
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PMC9268183
Approximately, 60% of the drugs used to treat cancer are of natural origin.
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PMC10335954
The human gastric malignant cell lines SGC-7901, AGS, MGC-803, BGC-823, and MKN-45 were obtained from Shanghai Genechem (Shanghai, China), and MGC-803 and AGS, which have been extensively studied.
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PMC8873393
Data are means ± s.e.m. (
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PMC11615828
Furthermore, Rab5b partially overlapped with Rab7a‐ and LAMP1‐vesicles, respectively (Figure S15a, arrowheads, Supporting Information).
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PMC11803149
CD8 T cells were isolated from spleens of young OT-I mice (OT-I T cells) and were nucleofected with Crispr ribonucleoproteins targeting Epas1 (Epas1-Crispr) or control ribonucleoproteins (control-Crispr) on day 0, before being cultured overnight.
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PMC11486946
Viability assays were carried out under 10 μM of compound for 72 h. The left panel in Fig. 5a shows that all hit EGFR-TKIs (i.e., afatinib, erlotinib, gefitinib, lapatinib, ponatinib, vandetanib, dasatinib and ibrutinib) reduced the viability of A431 cells and EGFR-transfected Ba/F3 cells, consistent with a dependency ...
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PMC11521786
This adjuvant combination in a human self-antigen-based vaccine has also been shown to induce higher antibody titers compared to using these adjuvants alone (41).
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PMC10882807
In our study, we found that EIF4A3 was remarkably overexpressed, and the expression of EIF4A3 was positively correlated with the expression of hsa_circ_0005397.
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PMC11674146
In our current study, we focused on the PI3K/AKT pathway, whose interaction with CLP36 has not been explored.
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PMC10882807
The primers used in this study were as follows: hsa_circ_0005397 (Forward: 5′-GACAAAGACAGCA GGTTCCT-3′; Reverse: 5′-CTC​TGT​TCT​GCT​TCT​GAG​TA-3′); EIF4A3 (Forward: 5’-CAGGGCGTGTTTTTGATATGAT-3’; Reverse: 5’-ATCAGCTTCATCCAAAACCAAC-3’; RHOT1 (Forward: 5’-CTGATTTCTGCAGGAAACACAA-3’; Reverse: 5’-GCAAAAACAGTAGCACCAAAAC-3’); ...
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PMC11543853
Thirty minutes before infection, wiskostatin was added to the cell media to a final concentration of 50 µM. Subsequently, the cells were infected with echovirus 18 with a multiplicity of infection of 1–2.
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PMC9429973
Finally, modeling analysis showed that the CT/TT genotypes exhibited a lower methylation and a higher CAT expression level, suggesting that the rs1001179 T allele and methylation cooperate in inducing CAT gene expression.
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