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PMC11190538
The radical cation was prepared by combining a 7 mM ABTS stock solution with 2.45 mM potassium persulfate (1:1 (v/v)) and leaving the mixture for 4–16 h until the reaction was complete and the absorbance was stable.
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PMC11248911
Moreover, the promising derivative was capable of arresting cell growth at G1 phase and inducing early and late apoptosis.
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PMC9424261
The data were analyzed using Student’s t test and shown as the mean ± SD from three biological replicates. *
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PMC11607638
Here, we identify the A. aegypti sulfakinin receptor gene SKR from the A. aegypti genome and show that SKR is expressed at different developmental stages and in varied anatomical localizations in the adult mosquito (at three days after eclosion), with particularly high expression in the CNS.
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PMC10891340
Our results showed that the dose-dependently CCCP treatment resulted in a decrease in the number of fluorescent spots of DPV and a significant reduction in the area of the viral plaque, suggesting that CCCP influence the intercellular transmission thereby inhibiting the spread of virus and viral replication.
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PMC10988555
H NMR [(CD3)2CO, Me4Si, 400.13 MHz] δ/ppm: 9.50 [d, 2, H1, JHH = 4.38 Hz]; 8.21 [s, 2, H4]; 7.68 [m, 3, Hpara(PPh3)]; 7.63 [m, 2, H2]; 7.54 [m, 6, Hmeta(PPh3)]; 7.28 [t, 6, Hortho(PPh3), JHH = 7.28 Hz]; 6.56 [s, 5, HCp]; 2.56 [s, 6, H6].
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PMC10094712
At this time, the cells are in the active stage of proliferation.
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PMC11406030
Protein concentration was determined by the Pierce BCA protein assay (23225, Thermo Fisher Scientific), following the manufacturer’s protocol.
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PMC8753818
All assays were performed in triplicates and repeated.
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PMC11730311
To gain further understanding of the A36S mutation, we used Schild regression analysis to study how the A36S mutation affects antagonism of hαCGRP8-37 with and without N25K-C20DA-γGlu conjugation (Fig. 10a,b).
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PMC11352311
The proteins were separated on pre-cast gels and transferred to a PVDF membrane using a rapid transfer buffer.
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PMC11116779
All quantitative results were analyzed using Graph Pad Prism Software (version 9.00 for Mac; GraphPad, San Diego, CA, United States).
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PMC11392912
The blot was then incubated overnight with 5% BSA at 4°C followed by 2 h incubation at RT with anti p-65 primary antibody (Santacruz, U.S.A.) and loading control GAPDH with 1:5000 dilution each.
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PMC11591794
The STRING database provides descriptions of protein–protein interactions (PPIs) as well as confidence intervals for data scores.
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PMC11747949
However, the VEGFR signal decreased statistically under CCR2 inhibition (Figure 3F).
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PMC11747467
All data in this study were analyzed using GraphPad Prism and R software.
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PMC11541241
The homologous proteins of the components of TORC1 are found in all the investigated fungi.
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PMC7504302
This analysis revealed that the high expression levels of RAN, RUVBL1, and SNRPD1 genes were significantly associated with lower overall survival rates (Figure 2).
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PMC11478724
After 48 h, ROS production was 3.366-fold higher than in control cells and the LDH release rate was increased by 39%.
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PMC11720808
In previous studies, some compounds were found to significantly regulate both TLR4 and TRAF6 expression, resulting in changes of NF-κB activation,27,33 but in the present work, we found that the expression of TRAF6 was not consistent with the expression of TLR4 under SCU treatment (Figure 3A and B).
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PMC11279598
Accordingly, additional work is needed to define the benefits and mechanism of action of MH and its potential for use in the clinical management of individuals afflicted with specific types of cancers, particularly breast cancer.
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PMC10873328
The RNA expression and clinical data of HRGs were systematically evaluated in OC training group.
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PMC10976516
Both murine tumor cell lines delivered high doses of VSV, even when recipient tumor-bearing mice were pre-immunized against VSV.
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PMC10079526
Upon replication fork stress, SMC5/6 SUMOylation and Nse2 SUMO-ligase activity are involved in the resolution of blockages preventing replication .
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PMC9444294
Five different synovial sarcoma cell lines were used: HSSYII (RIKEN, Saitama, Japan), SYO-1 (Dr. Akira Kawai, National Cancer Centre Hospital, Tokyo, Japan), Yamato (Dr. K. Itoh, Osaka Medical Center for Cancer and Cardiovascular Diseases, Japan), MoJo (Dr. K. Jones, University of Utah, Salt Lake City, UT), and Fuji (D...
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PMC11615743
After removal of the tube, the DNA products were precipitated via centrifugation at 12 000 rpm for 5 min.
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PMC9429973
Pts are assessed post treatment for up to 5 years for survival.
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PMC10978090
Patient underwent cardiac surgery with CBP on D17 with UFH 20,000 IU and aspirin 300 mg without thrombotic complication.
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PMC9429973
Additionally, we found that most differentially expressed genes show the most extreme response in the HSCs.
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PMC9429973
Methods: Investigating a cohort of 454 intensively treated CEBPA mutated AML pts entered into the AMLSG BiO Registry study (NCT01252485).
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PMC10914904
Therefore, understanding the roles of these svtRNAs in innate immunity could provide new insights into the roles of these noncoding RNAs in the inflammatory process.
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PMC11206251
Based on the results of the MTT assay study, the PEIM showed cytotoxicity against HepG2 cells, and the IC50 value was 55.03 μg·mL. Moreover, the results also showed, by Hoechst33342 staining assay, that the PEIM could cause HepG2 cell lysis and death, which was used to verify whether the PEIM could affect the morpholog...
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PMC7709199
As illustrated by the case study on DSCAMIg7–Ig9, it is possible to obtain well proportioned crystals from wild-type HEK cells that unfortunately do not diffract X-rays well.
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PMC9910796
The nature of the polyprotein-derived mature products composing this inner capsid remains unknown due to insufficient resolution of the available three-dimensional reconstructions.
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PMC10919056
PFS analysis between the high-RS score group and low-RS score group. (
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PMC10297204
Fifth, there is a possibility that these effects might differ between mice and humans.
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PMC6901583
Fig. 2BRD4 promotes GIST cell proliferation, migration, and invasion.a GIST882 and GIST-T1 cells stably expressing vector or BRD4 were harvested.
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PMC10778532
Our data indicate that the mutation C162F confers a significant higher healing ability by comparison with WT VHL and EV cells.
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PMC5173112
In this study, we showed that USP21 is enriched in CSCs population of RCC.
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PMC11615743
Our aim was to employ L‐DNA probes to analyze the unique expression patterns of multiple cell surface proteins to identify cell subtypes in complex environments.
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PMC11474209
MC-LR is toxic to both developmental and reproductive health.
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PMC11078693
Fill the tube with 50 mL of 1× PBS.
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PMC11185260
Moreover, current treatment options often cause nonspecific cytotoxic effects that negatively affect the development of the young patient’s brain, resulting in long-term neurological deficits.
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PMC10606998
The application of SPIONs for radiosensitization further expands the possibilities for their combined multipurpose use in biomedicine.
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PMC11301242
By integrating RNA sequencing (RNA-seq), H3K4me3/H3K27ac chromatin immunoprecipitation sequencing (ChIP-seq), assay for transposase-accessible chromatin using sequencing (ATAC-seq), and high-throughput chromosome conformation capture (Hi-C) data, we discovered that ecDNA amplifications contribute to the efficient, copy...
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PMC11621565
Based on the MTS and LDH end-point assay results, several concentrations were chosen to evaluate their effects for 120 h, such as 10 µg/ml, 40 µg/ml, and 80 µg/ml of rhamnolipid (A), 1 µg/ml, 32 µg/ml and 64 µg/ml of LPS (B), 16 µg/ml of curli CsgA (C) and 16 µg/ml of PSMα1 (D).
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PMC9429973
In vitro, both azacitidine and venetoclax significantly enhanced the pro-phagocytic effect of TTI-622 on macrophages.
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PMC11244823
Somewhat more surprising were the observations that not even the presence of antibodies against gG1 or history of repeated vaccination against EHV-1 stratified with the “true positives” against gD1_83.
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PMC11574271
This effect was attenuated by knocking down MMP12 (Fig. S1C, D).
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PMC7192625
Expression is denoted by node color (yellow RPKM < 1, blue RPKM > 1, (32)). (
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PMC4112127
However, after an additional day of incubation with TSA the expression of the BMP3 mRNA was increased 5-fold in the KMC-1 cell line and 8-fold in the Mz-ChA-1 cell line (Figure 4A & 4B).
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PMC9221284
We posit that this comprehensive characterization of c-Myc transcripts will contribute to reduced expression of oncogenic c-Myc forms in a targeted manner.
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PMC10778532
This was associated with ZHX2 inhibition and downregulation of P-ERK.
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PMC11782508
Prior studies have identified the azurin gene in environmentally sourced P. aeruginosa strains.
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PMC9960565
The metallodendrimers showed a strong binding affinity with DNA compared to cisplatin (Table 7).
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PMC7039683
Box and whisker plots of deltaSVM scores of 1000 randomly-selected SNPs near KRT18, scored by classifiers trained by zGPAEs (zebrafish periderm active enhancers), hOEAEs (human oral epithelium active enhancers), mPEAEs (mouse palatal epithelium active enhancers) and mPMAEs (mouse palatal mesenchyme active enhancers).
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PMC11531744
We also predicted the correlation between drug sensitivity of targeted drugs and commonly used chemotherapy agents and patterns of 25 IRGs expression involved in the construction of prognostic models for ovarian cancer (Fig. 6 and Fig. S3).
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PMC11525293
To verify this hypothesis, we reduced ΔNp63α protein levels by transfecting HaCaT and A431 cells with the CRBN encoding plasmid.
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PMC10957991
After 12 days in culture, cells were lysed for Western blotting.
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PMC11699465
Using different experimental methods, either in fixed cells or in live cells, the nuclear localization of TMEM199 was finally verified.
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PMC11711127
Overcoming challenges related to their function, numbers, and the intricate interplay with the local environment becomes vital for successful therapeutic outcomes.
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PMC9786247
NMI significantly inhibited E. coli LPS-induced expression of IL-1β, IL-6 and TNF-α (Figure 4a).
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PMC5941560
We detected the same two heterozygous variants.
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PMC9684669
Western blot analysis of hERG channel (KCNH2) and HRH1 in MDR ovarian cancer cell and its sensitive parental A2780 cell. (
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PMC11522251
12–14].
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PMC9429973
We are interested in studying the clinical and biological characteristics, the different treatments received and overall survival (OS).
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PMC11680562
Notably, a significant peak at 426 cm was observed, attributed to the presence of Cu–O bands, affirming the formation of CuO NPs.
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PMC11496491
The integrity of total RNA was detected by electrophoresis using RNA specific agarose.
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PMC11543885
Assign confidence scores to the proximity labeling-MS data using the computational tool-SAINTexpress via the online Contaminant Repository for Affinity Purification (CRAPome) platform at www.crapome.org.
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PMC11098378
D. Venn diagram showing conserved putative substrates (overlapping region) of IpaH4 across UBAIT experiments (n = 3) in LD652 cell lysates and Y2H screens (n = 2) against a human prey library.
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PMC11536589
RNA-seq was performed between AsPC1 cells and AsPC1-MR cells.
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PMC9429973
Results: Only 1 DLT (autoimmune hepatitis [grade 4]) was observed among the first 6 patients from all cohorts included in part 1 at the favezelimab 200 mg dose; thus, the dose was escalated to 800 mg.
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PMC11763111
They also showed enhanced proliferation upon antigen stimulation (Fig. 2C).Fig.
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PMC9429973
Two pts had KTE-X19-related Grade 3 serious infections: pneumonia and upper respiratory tract infection (n=1); influenza (n=1).
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PMC11727062
Some studies have found that DM-1, as a derivative of curcumin, can achieve antitumor effects by down-regulating the activities of matrix metalloproteinases (MMPs) that are associated with aggressiveness, such as MMP-2 and MMP-9.
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PMC9429973
EAIRs for grade ≥3 AEs and AEs of clinical interest of any grade were lower with I+V than with FCR (Table).
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PMC9429973
Methods: Retrospective study of 142 patients over 60 years with hematological neoplasms undergoing allogeneic HSCT.
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PMC7709199
Both cell lines produced about 3 mg of CSF-1RD1–D3 per litre of culture after purification by IMAC and SEC.
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PMC11657695
Recently, a meta-analysis study identified KCNA2 as predominantly expressed in skin cutaneous melanoma (SKCM), without observing a difference in expression with healthy tissue or a correlation with patients survival rate .
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PMC11687167
Systemic lupus erythematosus (SLE) is often considered a prototypic autoimmune disease.
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PMC9856122
However, naturally occurring isoquinoline and related alkaloids could be an alternative option for treating these rapidly mutating viruses.
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PMC9910796
At present, no sequence similarity has been found between pEP84R and other viral or cellular proteins.
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PMC11186948
Protein samples were finally denatured in Laemmli sample buffer 4x (LSB, #1,610,747, Bio-Rad) supplemented with β-mercaptoethanol and boiled for 10 min at 100 °C before they could be loaded on gel.
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PMC11594074
A DNMT1 activity inhibition assay revealed that compound 2 and compound 4 significantly inhibited the enzymatic function of DNMT1, exhibiting an inhibition at 10 µM of 46.4% and 55.3%, respectively.
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PMC11711935
The reduction of E‐cadherin can lead to melanocytes detaching from the basement membrane, subsequently resulting in melanocyte death.
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PMC11092382
Moreover, in the context of heart disease, sophocarpine not only ameliorates ischemia‒reperfusion injury resulting from postinterventional treatment for myocardial infarction (Li et al., 2011) but also exhibits a favorable mitigating effect on restenosis following revascularization.
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PMC11306331
n = 51,42,31,35,31,23 cells from conditions labeled left to right, pooled from three biological replicates.
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PMC3035438
The intronic region digested by BseRI was replaced by the human IgHG1 region from H to CH3 and a selectable marker.
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PMC6267596
Double Hit (DH) (germinal center origin) or Double Expresser (DE) (activated B-cell origin) DLBCL of comprise ∼20-25% of newly diagnosed high grade (HG) DLBCL and are defined by either translocation or over-expression of MYC and BCL2, respectively.
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PMC10125312
This is a limitation of the dose metrics typically used to express uptake and is a factor to consider during results extrapolation and standardization.
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PMC9429973
The sole event noted on both the PFS and OS curve is a pt death from heart failure.
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PMC9429973
Aims: To define the prognostic impact of albumin and CRP in the context of the MIPSS70.
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PMC11694625
The N atoms in 3b interacts with DG-8 and DG-24 bases to form hydrogen bonds (Fig. 8a), while 3c forms hydrogen bonds with DNA only with DG-8 bases (Fig. 8b).2ΔGb = −RT ln Kb Similarly, molecular docking is performed to predict the active conformation of a compound with target protein active sites and evaluate their bi...
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PMC11424574
P < 0.05 was considered a significant difference.
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PMC10237474
Zoom-in deconvoluted zero-charge mass spectra with a mass range of 52500 to 58000 Da are shown for three different retention times. (
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PMC11730320
B The CD4+ T cell CD45RA+CD28+ naive cell population is significantly low for age.
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PMC11306019
This could be a limitation of tetrazolium or resazurin-based cytotoxicity assays, where treatment duration is usually short (less than 3 days), often involving only a single dose, and therefore the effective concentrations might be higher than what is achievable in the human bloodstream.
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PMC11469524
p38 inhibitor responsive phosphosites are rather specifically regulated, with 70% (24/34) of significantly regulated sites neither co-regulated by other inhibitors nor counteractively regulated by α-IgM treatment.
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PMC11502962
However, not all the patients can benefit from the immune therapy.
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PMC10222971
Values were considered to be statistically significant when p < 0.05.
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