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PMC11194678
Statistics data were collected from triplicate experiments. (
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PMC7612475
It is well known that cancer associated SF3B1 mutations, which are almost exclusively missense mutations, do not result in the abrogation of SF3B1 expression, but rather change how SF3B1 recognizes 3′ splice sites, often leading to cryptic splice site usage, exon skipping and intron retention, which results in disrupti...
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PMC10040136
Overall survival curve analysis was performed by grouping all cases using the median of the proportion of MSC in the 50 samples as the threshold.
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PMC9429973
We performed in vivo serial transplantation and homing assays to study HSC re-population capacity.
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PMC11536589
After 60 h, the lentivirus containing medium were collected, combined, and cleared by centrifugation at 300 g for 10 min at 4 °C.
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PMC11794568
This finding provides a new perspective for targeted therapy of melanoma, and the targeting strategy against CRIP1 is expected to become one of the effective therapeutic means in the future.
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PMC8735881
CEMss-CCR5 cells were transduced with GPI-10E8/GFP and mixed with untransduced cells at a ratio of approximately 20% GFP-positive cells.
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PMC11222184
On the other hand, genes such as ATP5A1 and COX5A are associated with cellular respiration and ATP synthesis .
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PMC9429973
43 (64%) patients had at least one associated disease, with a CIRS ≥ 6 in 17 (25%).
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PMC4005030
Thus PCA was thought to be therapeutically useful to minimize age-associated disorders where oxidative damage is the major cause.
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PMC11593084
Interaction with ERM proteins is crucial for PDPN-induced epithelial-to-mesenchymal transition (EMT) in tumor progression as well as lymphangiogenesis and immune responses .
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PMC10669128
An example of an in vivo visceral sarcoma model is represented by the MMTV-CR-1 transgenic mice generated by Strizzi and colleagues, in which CR-1 transgene expression is regulated by the MMTV (the mouse mammary tumor virus) long terminal repeat promoter and leads to uterine leiomyosarcoma development.
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PMC11612626
The ability of cells to maintain proteostasis and a healthy proteome gradually declines during aging (67).
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PMC9429973
Compared with pts who received BR, pts treated with zanubrutinib experienced greater improvements in HRQoL at Weeks 12 and 24 as reported on the QLQ-C30 (Table).
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PMC11657744
The differential effects observed suggest distinct functional roles of DA proteins in ciliary assembly and basal body anchoring.
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PMC11705862
The sections were mounted with DPX (Sigma Aldrich, #06522).
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PMC9509578
Cancer cells are known to utilize more aerobic glycolysis than oxidative phosphorylation due to high levels of glycolysis and poor transportation of metabolites from cells.
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PMC11525028
Half-life time calculated using a linear model (n = 3).(M) Diagram depicting the workflow of detection of ac4C site in ATF4 transcript using chemical reduction method.(N) Sanger sequence detected the ac4C site in ATF4 transcript (C > T misincorporation).(O) Misincorporation rates of ATF4 transcript in control and NAT10...
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PMC11388384
Achieving this goal has been challenging in live embryos, because morphogens exist at low concentrations and access to highly sensitive microscopy is limited.
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PMC11599565
We detected argentaffin cells in the ovarian surface epithelium of regular tissues (Fig. 7, Panel B, upper block of histochemical pictures, OR -ovarian regular-, A-D).
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PMC10988555
The P NMR spectra are in good agreement with these findings.
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PMC11435360
The BBN PAG + NaHS group had 40% normal tissue, whereas the BBN GEM and BBN PAG + GEM groups had 16.7% and 50% normal tissue, respectively (Figure 3C).
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PMC9429973
5/21 patients with D5PSS4 (24% of total) actually received RT, without significant difference based on the “1.4” criterion” (29% vs 17%, p=0.54).
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PMC9429973
Treatment for 2 years at a dose of 20 mg/kg/day in young infants (median age 15 months) was also reported to be well tolerated and effective in improving Hb and HbF levels and delaying functional asplenia.
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PMC11637284
The kDNA is constitutively linked to the tripartite attachment complex (TAC) ‐ a physical structure which extends across the IM and the outer membrane (OM) to the basal body (BB) of the single flagellum (Figs 1A and S1).
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PMC11297139
The nuclear condensates formed by ARID1A LLPS are significantly elevated in Ewing’s sarcoma patient specimen.
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PMC11588008
P < 0.01, vs blank group, P < 0.05 vs PM group.
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PMC11541409
When KIT/PDGFRA is completely inhibited at drug concentrations significantly above the IC50, most GIST cells undergo apoptosis.
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PMC9261184
The reliquefaction facility is operated when there is expected to be excess BOG generation for longer durations, for example, when the ship has stopped moving at the port and the main engines are switched off.
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PMC11612315
d Schematic illustration of cell-attached patch recordings.
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PMC11786156
The intercellular distances of extravascular and intravascular DLBCL cells were measured using ImageJ software (version 1.50i) (National Institutes of Health, Bethesda, Maryland, USA).
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PMC10530622
Enterobacteriaceae and Lactobacillus presence was distinctive in urine of stone-forming patients and controls, respectively .
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PMC11699042
The area under the curve of CN ratios was calculated using Graphpad PRISM 9, v9.4.1.
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PMC9429973
In some cases IgH::MYC translocations were not detected by RNAseq, but for these cases expression analysis showed high MYC expression indicative of the underlying IgH::MYC translocation.
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PMC9118379
Overall, a total of 302 variant PSMs corresponding to 105 variant peptides with 94 variant events passing a gFDR of 1% were obtained from the SSe-gFDR and CMSe-gFDR strategies.
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PMC6901583
Once the increased expression was observed, the possible role of BRD4 in disease advancement was studied next.
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PMC10996034
In the present study, the three Taxol resistance-related gene targets MMP1, ZYX and UNC5C in EOC A2780/Taxol cells were selected using RNA-seq and bioinformatics analysis, and validated in vitro in cellular experiments.
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PMC5308810
The data were analyzed with Kaluza analysis software.
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PMC11641630
After 3 h of incubation, cells were infected with BCG or M. brumae (MOI 10:1) as mentioned above.
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PMC11766174
Across multiple experiments, the quantification of CA-positive cells made by CA-ICC was associated with other viral markers quantified with validated methods (Figure 2, Figure 3, Figure 4 and Figure 5).
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PMC11513011
In addition, previous studies investigating the association between caspase-3 and the suppression of bladder cancer growth; for instance, as the caspace-3 for promising indicators of early recurrence in bladder cancer and apoptosis by Shikonin via caspase-3 in bladder cancer.
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PMC11319300
Next, we performed a comparative genomic analysis of additional species to determine if the MYEOV-3′-putative enhancer DNA sequence predated primates.
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PMC11290772
To faithfully replicate the clinical conditions encountered in ovarian cancer treatments, we elected to employ clinical primary tumor cells and organoids as our ex vivo models, rather than utilizing animal models.
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PMC11525028
B and C) MA plot (B) and volcano plot (C) showing the gene expression of osteosarcoma patient samples.
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PMC10154881
Then lymphocytes and DCs were mixed at a ratio of 10:1 (lymphocytes:DCs).
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PMC11767582
5-(4-ethoxyphenyl)-1-(3,4,5-trimethoxyphenyl)-1H-1,2,3-triazole (3j): Starting from 2g (73 mg, 0.5 mmol) and following general procedure D, compound 3j was purified by chromatography, eluting with n-hexane-EtOAc (2:1).
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PMC9429973
It is still present years after chemotherapy and possibly contributes to the outgrowth of a pre-leukemic clone, consistently generated in utero during fetal hematopoiesis.
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PMC9318744
ACE2 and TMPRSS2 are key receptors for SARS-CoV-2 entry.
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PMC11127909
However, no antitumor applications of CATR and BKA have been reported in the clinic, which might be due to their toxicity.
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PMC11001582
Sciatic nerves were exposed bilaterally, at mid-thigh level, severed and the distal stumps placed in contact with stimulating electrodes.
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PMC5311252
mRNA targets of specific miRNAs can be predicted based on complementarity between the miRNA seed sequence and the mRNA 3'UTR sequence.
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PMC6835428
In this regard, it is somewhat strange that particles containing the siBCL2 did not trigger any cell death, considering that BCL2 is an anti-apoptotic gene.
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PMC9429973
The primary endpoint was the rate of MR3 at 12 months (core phase), but all pts were followed for additional 2 years (extension phase) to describe the long-term efficacy and outcome.
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PMC9577200
According to the chemical structure, Sun et al. (2020) define graphene oxide (GO) as a honeycomb-structured carbon compound with hydroxide, carbonyl, and carboxylic moieties at its basal plane.
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PMC9429973
These effects on HUVECs were significantly reduced after their elimination by 14000 g centrifugation (Figure 1).
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PMC11697703
For each condition, a representative neuron is shown with and without dendritic tracing. (
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PMC11366496
Also, the chi‐square test, Pearson's correlation, and one‐way analysis of variance were conducted.
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PMC11470999
Additionally, PF downregulates the levels of integrin α6 and β4, inhibits cell survival and migration, and induces apoptosis, thereby suppressing the growth and brain metastasis of breast cancer.
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PMC11686380
GAP43 regulates axon elongation into the contralateral hemisphere during brain development. (
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PMC10142392
In vitro release studies of DOX from star-shaped copolymeric micelles have shown that this anticancer drug can be released over several hours.
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PMC7887261
All study subjects and biospecimens were collected with informed consent under institutional review board (IRB) approved protocols.
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PMC9429973
Aims: We aim to elucidate the spatiotemporal dynamics of hematopoiesis in the same individual in a murine HSC transplantation model.
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PMC11477621
C NMR (150 MHz, CDCl3) δ 173.8, 134.5, 129.0, 128.5, 128.0, 123.1, 120.7, 109.2, 100.4, 60.4, 46.5, 34.4, 30.2, 28.9, 26.8, 24.9, 21.5, 14.4.
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PMC10674574
The use of 25 model drugs during validation is intended to demonstrate the appropriate suitability and functionality of an in vitro model.
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PMC10936243
And, usually, it will depend on the surgery.
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PMC4443654
The shifting membership of these clusters evidently retains enough similarity between cell types to enable the cross-application of models.
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PMC11495567
(1:1000), Caspase 3 (Cat. #
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PMC10218459
The fluorescence signals of the cells treated with cytostatic drugs were normalized to the signals of the cells treated with argon and cytostatic drugs.
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PMC9429973
Results: A total of 155 and 278 SSc patients fulfilled selection criteria were enrolled in our study from PKUTH-SSc and PKUPH-SSc cohort respectively.
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PMC11684269
Specifically, GLUT2 has a high affinity for glucose and oxygen.
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PMC11704135
The cells were maintained under standard conditions of 37 ºC in a humidified atmosphere with 5% CO2.
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PMC11286266
The eluted protein was then analysed either via immunoblot or Coomassie blue staining.
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PMC11291490
Given that the prM-E proteins constitutes the envelope of TMUV, a DNA vaccine expressing the envelope proteins of TMUV has been developed in the current study.
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PMC9429973
Furthermore, ACA turned out to be the most tolerated regimen, whereas the BTKis-O based regimens significantly carried a high toxicity profile as compared to BTKis alone, counterbalancing the advantage of O-addition in terms of ORRs, where O-based regimens achieved the higher rank, when combined either with VEN or BTKi...
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PMC11267036
The execution process was in accordance with previous research .
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PMC11352311
On the SwissTargetPrediction website, select “Homo sapiens”, enter the isomeric SMILES, and click “Predict targets”.
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PMC8978939
Most of the effect prediction tools used suggest that both variants are deleterious.
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PMC8984067
Besides absorption peaks for characteristic PEI groups, there were also characteristic peaks for C=O, C-O, and amide N-H bending vibrations, which indicated that amide bond formation reaction occurred between -NH2 from PEI on the surface of UCNPs and -COOH from Ce6; thereupon, then the photosensitizer Ce6 was firmly an...
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PMC3274243
After dehydration in ethanol and critical point drying by liquid CO2, 6 nm of chromium was sputter coated onto each section of ceramic probe.
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PMC11461874
These results imply that TRAF7 could determine the peak level of DBP protein by promoting degradation of DBP.
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PMC11754436
Receiver operating characteristic (ROC) curve analysis.
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PMC9429973
All patients relapsed after chemotherapy, and the leukemia cells proliferated rapidly.
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PMC10197932
In this study, we investigated in vitro the bioeffects of low energy ultrasound on red blood cells and PBMCs isolated from healthy donors as well as on two myeloid leukemia cell lines (OCI- AML-3 MOLM-13) and lymphoblastic Jurkat cell line.
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PMC3035438
Further analyses (second and third blots) were performed after purification of IgG by affinity chromatography and dilution in PBS.
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PMC10667689
Also, this enzyme can be considered as a target for therapy, since inhibition of G6PD in AML results in cell cycle arrest or cell death and increased sensitivity in chemotherapeutic agents (80, 81).
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PMC9429973
T. Schramm, C. Ungerböck, M. Fillitz, B. Dixer, D. Mehic, C. Ay, T. Backchoul, J. Zlamal, I. Pabinger, J. Gebhart Department of Internal Medcinie 1, Division of Hematology and Hemostaseology, Medical University of Vienna; Department of Internal Medcinie, Hanusch Krankenhaus, Vienna, Austria; Transfusion Medicine, Medic...
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PMC11252553
In contrast, Taurine’s action in combating free radicals is indirect and likely involves different approaches to counteract oxidants.
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PMC9429973
Continuous variables were compared with the Mann–Whitney test, survival data were analyzed using Mantel-Cox test.
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PMC11748363
Infectious diseases, including bacterial, fungal, and viral, have once again gained urgency in the drug development pipeline after the recent COVID‐19 pandemic.
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PMC10142392
Although it is possible to produce PCL by the direct condensation of 6-hydroxycaproic acid, the most common method for large-scale synthesis of high molecular weight and low dispersity polymers is the ring-opening polymerization (ROP) of ε-caprolactone.
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PMC11706776
To compare two groups from in vitro experiments, Mann–Whitney tests were performed and ANOVA tests were administered when N > 30 and a normality test had been passed.
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PMC8270637
These lymphocytes recognize tumor antigens and subsequently release granzymes and activate Fas/FasL pathways to promote tumor cell apoptosis, or exert direct/indirect anti-tumor effects through the release of cytokines (24).
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PMC9429973
Here, we described the clinical and genetic features of MPN patients with concomitant MGUS.
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PMC7812570
Fig. 1Cell-surface expression of GPI-anchored antibodies in transduced TZM-bl cells.
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PMC11711127
Therefore, it was proposed that NK cells induce T cell anergy upon HINT1/Hsp70 treatment through GRAIL expression (169).
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PMC11627398
Meanwhile, Liu et al. found that compared to stable coronary heart disease, LIAS and LIPT1 were notably reduced in AMI and suggested that LIAS and LIPT1 may serve as protective factors for AMI .
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PMC11371627
We display the restriction of Kell antigen expression resulting from the identified deletion to support its pathogenicity, and we review 44 further cases identified from the literature with multisystemic MLS adding to a better understanding of this rare disease with variable phenotypic presentation.
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PMC9688728
In the case of collaborations, cell passage number (or time of cultivation) and other conditions should also be kept the same .
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PMC9429973
In the context of OS and PFS, number of CD184+ cells was found to have a negative impact (correlation coefficient R = -0.32, p=0.0261, and correlation coefficient R = -0.41, p=0.0039 for OS and PFS retrospectively).
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PMC11721295
In the resulting L-iKRAS model (Ccsp-rtTa; TetO-Kras; Trp53), we induced KRAS expression by adding dox (Sigma-Aldrich, D9891) to drinking water at a concentration of 0.5 mg/mL and mutant p53 expression by 1-time intranasal instillation of ad-Cre recombinase with 3 × 10 PFU/50 μL/mouse at study start.
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