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PMC11627060
Preclinical studies have demonstrated TQ pleiotropic properties, highlighting its role as an antioxidant, immunomodulator, and anticancer agent .
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PMC11594641
The level of N-cadherin did not change significantly, which is in line with the result of the invasion assay (Figure 7E,F).
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PMC11683240
Values of median are given as x-fold of the untreated control (Con), mean ± SEM (n = 9 values from three independent experiments).
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PMC11577421
Enzyme activity was confirmed through immunofluorescence in separate control experiments using anti-HS and anti-CS antibodies (Suppl.
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PMC10955424
Secondary antibodies, HRP-linked anti-rabbit (#7074, Cell Signaling Technology) and anti-mouse (NB7539 Novus, Centennial, CO, USA, dilution 1:2500) were used according to the manufacturers’ instructions.
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PMC11521786
All of these factors emphasize the importance of developing an effective HER3 targeting strategy.
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PMC9516401
The result of yield of extracts is shown in Table 1.
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PMC11632064
In response to BI 6727, PARP cleavage and β-actin degradation were higher in A-375 cells than in MeWo cells (Fig 4E–G), suggesting that cell death in A-375 cells is related to the induction of apoptosis.
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PMC7338939
Samples were acquisitioned by BD FACSCalibur in each step and analyzed by flowing software.
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PMC11640247
Recombinant His-tagged RAC3 and RAC3-R66W proteins were expressed and purified according to the manufacturer’s guidelines.
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PMC7823217
Selection of these normal cells in bioinks should be based on the tumor characteristics and bioengineered accordingly.
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PMC6642070
β-enolase is the muscle-specific isoform of enolases, converting 2-phosphoglycerate to phosphoenolpyruvate in the glycolysis pathway, and is expressed higher in type II muscle fibers than type I fibers .
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PMC11798926
This was done in part to exploit the high conservation between SynDIG1 and SynDIG2 to map the noncanonical endocytic signal in SynDIG1 (Figure 1A).
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PMC11656048
These results suggested that shikonin might interact with Nrf2 proteins and contribute to the ubiquitination degradation of Nrf2, thereby inhibiting downstream xCT and GPX4 expression.
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PMC11774747
One patient relapsed after 4 months and a second developed a Richter syndrome after 8 weeks.
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PMC11665584
Overall, PITX1 keratinocytes had higher oral pseudotime gene expression scores, further increasing as they differentiated, supporting the key role in driving oral epithelial identity and oral keratinocyte differentiation by PITX1.
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PMC9429973
Summary/Conclusion: Overall, the higher the GI-GVHD histological score at diagnosis, the higher the NLR and the lower the LMR.
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PMC6379402
The plots are generated at the Human Protein Atlas web-server Finally, we observe that only 17 (27%) of the 63 prioritized genes are significantly differentially expressed in cancer tissues with respect to the paired normal tissues (using expression data from TCGA projects (The Cancer Genome Atlas, http://cancergenome....
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PMC9581083
Therefore, TCM can play a multi-target role in the regulation of body systems (Zhong, et al., 2019).
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PMC11701801
Simultaneous inhibition of CBP/p300 causes synthetic lethality in SMARCA4/SMARCA2-deficient and SS18–SSX fusion cells.
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PMC10568263
BSA was used as a blocking agent to mask non-specific active sites.
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PMC6222635
From here, we can confirm that C1 of (1→4)-linked glucose is connected with C4 of the (1→4)-linked mannose.
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PMC11321678
Evidence indicates that Group 3/4 cells originate from progenitor‐like cells in patient A7.
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PMC11201245
In this study, 30 OCs were tested for genomic imbalances by aCGH.
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PMC9429973
AutoHSCT may offer prolonged survival in selected cases of AITL but relapses are common.
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PMC9784246
The medium containing the L. major cells was transferred to a Falcon 50 mL sterilized conical centrifuge tube and centrifuged for 15 min at 5000 rpm until the cells were completely settled, the old medium was aspirated, and 5 mL of fresh medium was added to the settled cells, which were counted under the microscope and...
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PMC11045125
In contrast, cyclin D2, which is expressed in EBV-transformed LCLs but not in EBV+ BLs , is turned down in the ΔEBNA2 + Myc lymphomas (Fig 5A).
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PMC11738017
Generally, target binders in CAR T cells are designed to have a low binding affinity and short KD off rate to enhance the serial killing of target cells and to minimize T-cell exhaustion.
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PMC11139449
It allows for larger DNA fragment insertions (up to 200 kb), but optimizing insertion efficiency remains a complex challenge ( 12 ).
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PMC6461034
○Collect all remaining liquid from the storage tube by centrifuging for ˜20 s at 1,500 × g and add this to the OASIS HLB cartridge.
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PMC7192625
We considered contacts identified using PCHi-C (2), taking all interactions called by CHiCAGO as unweighted (see Materials and Methods).
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PMC11762280
Table 2Primer sequences in this study.
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PMC11706776
In addition, it has been demonstrated that the number of GGAA repeats in microsatellites influences the regulation of EWS::FLI1-targeted genes .
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PMC9429973
Summary/Conclusion: Quality of life of patients under anticoagulant therapy after venous thromboembolism, pulmonary embolism or atrial fibrillation was found to be moderately satisfactory.
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PMC10317042
To investigate the antitumor effects of vorinostat on CCS, we performed a cell viability assay using four CCS and NHDF cell lines.
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PMC10976516
These studies demonstrated the potential of immune cell carriers of OVs and should be followed up with further (pre)clinical tests in suitable lung tumor settings.
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PMC11033180
With the aim of studying the effects of Sema5A on enteric neuronal connectivity using in vitro ENS culture models, we first validated the expression of Sema5A and PlexinA1/A2 in a widely used ENS culture model derived from embryonic rat gut.
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PMC11055443
Soluble factors of AD-MSCs reduced V. cholerae colonization on the surface of Caco-2 epithelial cells.
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PMC10938336
The assessment of the druglike potential of the active compounds is a critical starting point.
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PMC11047729
or high (2c.)
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PMC11483468
Chen et al. reported that the C-terminal 290-328 amino acid (aa) was the core region that inhibited telomerase activity, and the N-terminal 1-289 aa had no obvious inhibitory effect (23).
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PMC11790971
The classification results demonstrated the potential of this approach to effectively differentiate between CHO cell lines in early passages.
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PMC11300639
Similarly, we revealed that RNF26 knockdown decreased VEGFA expression and S6K1 T389 phosphorylation in both 786-O and A498 cells (Fig. 4e, f and Supplementary Fig. 2c).
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PMC10094992
In this article, we describe the expression and role of ALDH1A3 in different MPM cell lines cultured as multicellular spheroids (MCSs).
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PMC11657695
E YAP protein expression in A-673 and SK-ES-1 lines transfected with the siRNA CT or a pool of siRNAs targeting KCNA2 (siRNA KCNA2) with the normalized and relative quantification (N = 4 for A-673 and N = 6 for SK-ES-1).
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PMC11718109
The large vesicles were positive for VMAT2 in DA neurons and in an SV-like cluster reconstitution system in fibroblasts.
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PMC11727145
The best results were obtained with media compositions containing both insulin and EGF, which demonstrated high cellular proliferation and invagination comparable to the use of mGreen’s medium for the air–liquid interface (Figure 4, #8–10,G).
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PMC10530622
In addition, the exposure of NRK-52E cells to oxalate favored the adherence of CaOx crystals to the cells.
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PMC9429973
Limited evidence points that using markers of disseminated intravascular coagulation (DIC) might predict thrombosis in AML, and the Khorana score is not useful for this purpose.
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PMC11367146
The multi-component and multi-pathway features of the phytoconstituents present in VTE, and their mechanism of action were elucidated.
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PMC11128598
We found that both inhibitors exert and antiproliferative effect in all the cell lines analyzed, and when combined with CQ a potent synergistic effect was observed, except in LN-18, one of the two glioblastoma cell lines studied.
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PMC11741906
Here, the present study is an exploratory and primarily based on in vitro models investigated the miRNA expression changes related to cisplatin in the ovarian cancer cell lines A2780, A2780cis, and SK-OV-3.
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PMC11228511
High-magnification images of the boxed regions are shown at the Bottom. (
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PMC11549612
In ovarian cancer, elevated expression levels of BOLA2 and BOLA3 have been observed, indicating their potential as biomarkers for the disease.
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PMC9429973
Results: Patients with HABMF had a significantly lower proportion of CD3+ T cells in their BM compared to the nonHABMF with the predominance of CD8+ T cells and their activation by expression of HLA-DR observed with flow cytometry.
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PMC9429973
Groen-Damen, M. Semerjian, V. Strulev, J. M. Schecter, T. Roccia, T. Nesheiwat, R. Wapenaar, K. Weisel, M.-V. Mateos Universitätsklinikum Würzburg, Medizinische Klinik und Poliklinik II, Würzburg, Germany; University Hospital Hôtel-Dieu, Nantes, France; Section of Hematology, Catholic University, Fondazione Policlinico...
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PMC11802855
The bone marrow samples of patients were collected at the time of diagnosis.
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PMC10094992
NR6 induced senescence in CDKN2A proficient cells, while induced apoptotic death in CDKN2A silenced or homozygously deleted cells.
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PMC11670954
These findings provide a critical theoretical foundation for elucidating the impact of BA on LPS-induced barrier damage and for developing novel clinical therapeutic strategies for intestinal injury.
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PMC11699042
Red dots denote significant DEG (BH adjusted P < 0.01; |FC | > 1).
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PMC11774747
This suggests that the therapeutic potential of these CAR-VSTs may be optimized by adjusting the dosing regimen (98).
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PMC9429973
PETINT(+) patients had a median PFS of 31 months compared with a median PFS of 88 months in PETINT(-) (p < 0.001), Figure 1.
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PMC8427838
After release via boiling the samples for 5 min at 95 °C, the immunoprecipitated proteins were subjected to western blot analysis.
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PMC11656048
In this study, we present significant evidence supporting the effective antitumor activity of shikonin against MG63 and 143B cells in vitro, significantly inhibited the rates of proliferation and markedly induced cell cycle arrest in dose-dependent manner.
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PMC11049548
To this aim, we analyzed and compared via H NMR the intracellular and extracellular metabolome of HGSOC cells derived from (i) macroscopically cancerous solid tissue (HGSOC_T) and (ii) ascites (HGSOC_A) collected from HGSOC patients at the time of cytoreductive surgery with those of A2780 and A2780 cisplatin-resistant ...
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PMC6461034
Leave for 10 min at room temperature and mix gently every 2–3 min.
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PMC11243198
BAS 07019774 significantly reduced Gli1 mRNA expression at both time points (4.9-fold at 48 h and 2.2-fold at 96 h) compared to the SAG-treated cells (Figure 4A,B), while Z27610715 showed a significant reduction only at the later time point (4.5-fold at 96 h) (Figure 4B).
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PMC11502443
The Emax for tirzepatide-stimulated pAKT, but not pERK1/2, was lower at the E354Q receptor (Table 1).
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PMC11599565
Consequently, we identified proteins involved in the global transcriptional regulation induced by cisplatin in the SKOV3 ovarian carcinoma cell line.
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PMC11541241
Recently, scientists found that a rice FKBP12 homologue functioned as a negative regulator in plant biotic stress response (Cheung et al., 2020).
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PMC6617630
Bromodomains recognize acetylated lysine in histone tails and activate transcription and function as epigenetic readers.
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PMC9545474
To gain an insight into the possible MoA of the most active complexes 6 and 8 reported in this work, apoptosis studies and flow cytometry analysis of treated A2780 cells were carried out.
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PMC9429973
Both these mutations lead to a truncation of EPOR.
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PMC9429973
A total dose of 10mg/kg ATG- Genzyme or 20mg/kg ATG- Fresenius was given from days -5 to days -2.
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PMC10812665
Data on transcriptome changes were obtained for three exposure times.
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PMC11506670
For inverse PCR site-directed mutagenesis, the PCR reaction mix was initially denatured at 98 °C for 30 s. This was followed by 30 cycles of denaturation at 98 °C for 10 s, annealing at 65 °C for 10 s, extension at 72 °C for 3 min, and a final extension at 72 °C for 5 min.
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PMC9611901
Rather, it is a warning sign indicating that chemicals released from a tested sample have a high potency to produce DNA lesions that can be specifically detected by the alkaline comet assay.
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PMC9503669
Early studies in non-human primates provided strong evidence that Nef is required for SIV and HIV-1 pathogenesis and the development of AIDS.
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PMC10968586
Malignant pleural mesothelioma arises from mesothelial cells and is characterized by a pronounced aggressiveness and poor prognosis.
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PMC11650848
We used a commercially derived cell line (HCT116) and its DKO counterpart alongside the neuroblastoma cell line (SH-SY5Y) and its Aza-treated counterpart.
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PMC9429973
Comparing the cytokine responses of the HD patients (n=8) with the healthy controls (n=22) did not result in any significant differences.
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PMC9532503
These mAbs are advantageous over rituximab and are currently undergoing clinical investigation.
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PMC11495567
The number of vinculin punctates and area occupied by the vinculin punctates per cell were quantified using the BZ-800 analyzer and graphed using GraphPad Prism 8.4.3.
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PMC11463018
The detailed protocol can be found in Ref.
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PMC10222971
Experiments were performed in triplicate; data are expressed as the mean of triplicate measurements (mean ± SD). %
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PMC11737091
Univariate Cox regression analyses revealed that DBF4 expression was a risk factor for patients with ccRCC, as were age, clinical stage, T stage, N stage and M stage (Table 1).
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PMC9429973
4 AEs grade 5 related to VEN was observed in 3 patients (deterioration of general condition, ascites, hepatic failure, death).
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PMC11792766
No. 93813‐100MG), Mitomycin C (Mit C) (Sigma Aldrich, Cat.
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PMC11085211
The entire sample was then centrifugated and resuspended in 500 µL of fresh medium.
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PMC11750165
When PEX1 was cotransfected with full-length of Jagged2, its immunoreactive band was strongly detected in Western blots by probing with anti-Flag antibodies (Fig. 1C).
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PMC10040136
If low doses of rapamycin can effectively counteract GIST resistance, a novel therapeutic approach may involve combining rapamycin with Imatinib.
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PMC11566417
In fact, the morphology of late-passage primary MDECs, characterized by larger and rounder more spread-out cells, resembles the commonly observed morphology of cells that lose their proliferative capacity, which includes a “flattened” appearance and an increase in cell size .
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PMC9429973
We therefore need objective parameters to evaluate and guide our transfusion strategies.
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PMC11283030
Furthermore, GEPIA2 analysis validated the tight relationship between ACLY and the four immunoinhibitors in 523 ccRCC tissues (Fig. S5).
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PMC6163708
Our results suggest which type of cancer is resistant or sensitive to GANT61 when it is applied directly on cells in culture (Figure 2).
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PMC11791264
MYC (MMS-150R) antibodies were purchased from Covance.
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PMC11248911
S-Alkylation was the first step in these reactions, followed by dehydration or the loss of an alcohol molecule.
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PMC9429973
Finally, ACA monotherapy is the most tolerable therapy (SUCRA value 100%, probably the best 100%), whereas IBR-O turned to be the most toxic treatment (SUCRA value 0%, probably the best 1%).
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PMC10899471
B, C) qPCR and Western blot analysis of BATF2 expression in HT-1080 cells transfected with pControl or pBATF2 treated with control or miR-939-3p mimic. (
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PMC11301242
Among the 55 identified ecDNA amplicons, 268 genes were detected, of which 38 are known cancer-related genes exhibiting significantly increased copy numbers.
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