PMCID
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PMC9429973
Overall, 68% of pts reported dissatisfaction with their current treatments for SCD.
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PMC11539788
Moreover, the enhanced malignant behaviours induced by RNF19A knockdown were attenuated after downregulating ILK, as confirmed by CCK-8, wound healing, and transwell invasion assays (Supplementary Fig. 4C-D,F,H).
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PMC10079526
Trapped DNMT1 is degraded by the proteasome in a SUMOylation dependent manner in HeLa cells .Fig.
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PMC11788920
Determining whether the principles derived from our studies on the c-Myc system are broadly applicable to other genes containing a PQS requires further research on additional gene systems.
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PMC11596967
In biological tissues, IR predominantly interacts with water, the most abundant cellular component, accounting for about 70% of cells.
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PMC11469524
In here we can recapitulate this finding on a larger core network (16 nodes, 16 edges) for aberrant and non-aberrant B-cell receptor driven signaling (Fig 6).
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PMC7351993
As shown in Fig. 1 and Supplementary Fig. 2b, the microfluidic chip contains a dual-membrane sorter, which allows for precise control of the push-pull sorting actuation.
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PMC10956161
The sections were then incubated with a goat antirabbit immunoglobulin G (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (catalogue number A-11008; Thermo Fisher Scientific; 1:100 dilution) at 37°C for 30 min.
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PMC11640476
After 11 days of growth in the fibrin gels, we evaluated the FI results in the identical samples treated 1, 2, 3, or 4 times with resazurin WS.
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PMC11721096
As shown in Figure 8A, it was surprising that S5 showed a degree of toxicity at low concentrations of 1–10 μg/mL, while it was safe at 25–200 μg/mL. Interestingly, at a high concentration of 100 μg/mL, S5 entirely recovered the intracellular tyrosinase activity and decreased the melanin production to a level lower than...
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PMC9429973
Aims: In this study, we compared blood parameters and RBC properties of SCD patients and compared the results between patients with asplenia, to patients with splenomegaly/hypersplenism.
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PMC11737091
Not applicable.
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PMC11790599
Untreated and TSL-treated cells were incubated with 1.25 mg/mL of lucifer yellow (LY, Sigma) in Hanks balanced salt solution (HBSS, Gibco) in a 37°C, 5% CO2 incubator.
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PMC9429973
Sole cutaneous bleeding was reported in 57 (46.7%) patients while epistaxis was the leading cause of mucosal bleeding occur in 30 (25%) patients followed by gum bleeding in 15 (12.5%) patients.
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PMC8010066
Quantitative real-time PCR was used to determine the expressions of lasR, lasI, algD, mexR, and KPC genes using the SYBR Green method, and rpoD was employed as the reference gene.
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PMC11574271
A two-sided Student’s t-test was used to compare the differences between the two groups.
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PMC11357960
Among these three genes, UBE2I had the best predictive ability (AUC = 0.733) for the resistance to anti-PD-1 immunotherapy.
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PMC8348645
Standard doxorubicin showed an IC50 value of 18.8 µM inhibition in the same.
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PMC10606998
However, despite treatment, the tumor quickly recurs, leading to the death of the patient .
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PMC9429973
Two decades of clinical trials have provided critical insight into differential efficacies and specific toxicity profiles of these TKIs supporting the development of guidelines such as the European Leukemia Net (ELN) and the national comprehensive cancer network (NCCN).
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PMC11585994
The percentage of apoptotic cells was detected by flow cytometry (left panel), with quantification shown in the right panel. (
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PMC9429973
C. Roddie, J. Dias, M. O’Reilly, M. Mitsikakou, E. Charalambous, L. Green, M. Vaughan, G. Agliardi, J. Garcia, E. Lewin, M. Lowdell, M. Marzolini, L. Wood, H. Holmes, Y. Ngai, B. Popova, W. Wilson, S. Kunaseelan, V. Spanswick, H. Lowe, L. Ensell, J. Hartley, S. Domning, S. Morley, T. Marafioti, A. Bloor, D. Irvine, K. ...
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PMC11794588
While C. parqui was found to contain saponins, C. diurnum was also shown to contain glycosides.
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PMC8427838
ERM proteins are highly conserved, possessing more than 75% amino acid homology within the common FERM domain .
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PMC11774747
However, a second signal (Signal 2) mediated by costimulatory molecules such as CD28 or 4-1BB is required for full activation, and to avoid anergy or apoptosis.
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PMC11599565
Wells were rinsed 3 × with PBST and mounted using Faramount Mounting medium (Agilent Technologies, CA, USA) for visualization in an Eclipse i-50 microscope (Nikon, Tokyo, Japan), using the NIS-Advanced imaging software.
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PMC11269792
Anti-IgG was the control for Anti-Ago2.
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PMC11753435
In this work, we provide an algorithmic framework to design short, target-binding linear peptides, requiring only the amino acid sequence of the target protein.
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PMC9479186
The cells counted as 4*10/ml.
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PMC11711063
Trypsin‐based adhesion assay after 48 h of ZG16B pre‐treatment.
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PMC11453018
A β-Gal and H&E staining in SKES1 cells at 24 and 72 hours of treatments with iP300w.
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PMC11679892
Then, the heating process was carried out gradually from 60 °C to 95 °C with 0.5 °C increments, and optical measurements were made at each 0.5 °C increment in temperature to create the melting curve graph via melting curve analysis.
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PMC11783017
After the K7M2 cells were treated with different materials for 24 h, DCs were collected and stained with PE-anti-CD80 and APC-anti-CD86, and then analyzed by flow cytometry.
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PMC11733919
TIS, therapy-induced senescence; 17D, 17-DMAG treatment; MNP@CD26, nanoplatform containing anti-CD26 antibody; MNP@CD26@17D, nanoplatform containing anti-CD26 antibody and 17-DMAG.
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PMC11741906
Median survival for 486 ovarian cancer patients was analyzed after categorizing samples into high (red) or low (black) expression groups for each miRNA (A–E).
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PMC11774747
The second strategy take advantage of expressing a truncated version of the epidermal growth factor receptor (EGFRt) for cells to be targeted and eliminated by cetuximab, a monoclonal antibody that induces ADCC.
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PMC9429973
Two patients discontinued oral Zanubrutinib due to oral ulceration and hematuria.
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PMC11672142
The cell line was cultured in phenol red DMEM (Sigma, D6429) containing 1% glutamine, 4.5 g/mL glucose, and sodium pyruvate.
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PMC10719213
Also, the distribution of mutations along the mtDNA sequence was similar in treated and nontreated cells (Fig. 5d).
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PMC9429973
The RDW, Hypo-He, and MCH cut-off values for discriminating IRIDA from TT patients were %19, %30, and 17.0pg, respectively.
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PMC11474209
For the toxin analyses, ELISA, LC/MS, and molecular screening for cyanotoxin-producing gene candidates were used11Lake water from Dongwazi Lake (Nanjing, China), drinking bottled water from the supermarketIC-ELISA-MscFv7-scFvWater sample0.471–0.548 µg/LA mouse phage scFv library was successfully developed with a capaci...
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PMC11743975
Upon blue light illumination, CRY2clust relocates from the cytosol to mitochondria where it induces mitochondrial aggregation by CRY2clust homo-oligomerization and CRY2clust-CIBN hetero-dimerization.
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PMC11599565
This indicates that the EpCAM positive cells are more resistant to agents like cisplatin.
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PMC10482220
All DNA manipulations were performed according to standard techniques.
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PMC10652261
Yield: 80%.
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PMC11738017
All other clinicians completing this activity will be issued a certificate of participation.
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PMC9429973
Karyotype and RT- PCR for BCR ABL1 at diagnosis were available for >75% of ptn in 40% of CC.
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PMC8343815
Furthermore, the production of secondary electrons by MV photons and nanoparticles near DNA may cause irreparable damage to cells and enhance the effect of radiotherapy .
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PMC11711871
Determination of the baseline levels of plasma PUFAs in hypertensive patients might serve as a diagnostic marker for a potential predisposition to developing salt-sensitive hypertension.
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PMC11604768
c Transcription rate estimates (aggregate RNA/DNA ratios) for CRE and Mafb units in HEK293 cells following 1 µM epinephrine treatment or ADRB2 overexpression, or both.
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PMC11188874
Lysates were centrifuged at 800 × g for 5 minutes and supernatant collected to which an additional 5 mL of lysis buffer was added and centrifuged at 20,000 × g for 60 minutes.
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PMC10547921
Their mode of action is selective alkylation in small grooves in DNA, where N2 of guanine forms a covalent bond with the electrophilic N10/C11 imine of PBD.
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PMC9428827
The increment on CD8MP cells but not in Tregs results in a change in the CD8MP/Treg balance toward immune activation ( Figure 2D ).
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PMC11522251
Subsequent research revealed that overexpressing HOXB8 enhanced the proliferation of colorectal cancer cells and considerably reduced cetuximab’s growth-inhibiting impact in SW48 cells (Fig. 5D).
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PMC7553912
The apparent molecular weights are indicated on the right.
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PMC11734514
Quantitative data of band intensity was measured with ImageJ ver.
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PMC8345486
Therefore, the concentration of cisplatin was 50–70 μM in some experiments with OVCAR-3 spheroids.
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PMC10040136
Apoptotic cells were identified using FITC-Annexin V and propidium iodide after GIST-882 cells were grown in control media or MSC-CM for 48 h at 37 °C (Cat No. 556547, BD Biosciences, USA).
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PMC6268318
Combretum micranthum is a bushy shrub or creeper found all over Africa.
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PMC7192625
Given the flexibility and efficiency of representing chromatin as a network, we have designed a pair of tools which can be used together or separately for the analysis of chromosome conformation capture data: GARDEN-NET and ChAseR (Figure 2).
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PMC11724582
TGF-β1 is expressed in almost all cells, including tumor cells and cancer-associated fibroblasts, and promotes fibrosis in a variety of tissues, such as the lungs, liver, and heart [31–33].
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PMC11092418
The cell migration assay was conducted using the IncuCyte Clear View 96-well cell migration plate according to the manufacturer’s instructions (Essen Bioscience Ltd. Hertfordshire, UK) as described previously .
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PMC9739791
The main hypothesis of the flavin-catalyzed photoreduction mechanism was that FMNH is formed from the reaction of FMN triplet excited state with electron donors such as NADH.
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PMC11786767
A: Representative images of mitotic chromosomes in prometaphase before and after expansion (Pre-Ex and Post-Ex, respectively).
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PMC11216402
In addition, the activity of transcription factors (TFs) regulating normal stomach and GC (GATA4, GATA6, KLF5, HNF4A, and EBF1) has been implicated in GC invasiveness using TF and histone ChIP-seq (Chia et al. 2015; Xing et al. 2020; Sheng et al. 2021).
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PMC5034105
Systematic sampling of domestic animals in ZIKV-affected regions would help to confirm these findings.
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PMC11033180
The Sema5A mutations described in ASD, S951C and S956G, were generated in pcDNA3.0 Sema5A-Fc with the QuikChange Lightning Site-Directed Mutagenesis Kit (210519, Agilent Technologies, les Ulis, France) according to manufacturer’s instructions and were verified by sequencing.
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PMC11770130
It has been reported that the homozygous depletion of CerS2 was detrimental to the overall health of mice.
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PMC11683240
Therefore, decreasing concentrations of purified recombinant proteins, Gαi and PTS1 (antibody control), α1AT, and PBS (protein-free negative control) were vacuum aspirated onto a nitrocellulose membrane.
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PMC11674891
Transwell chambers (Corning, New York, NY, USA) were coated with Matrigel Basement Membrane Matrix (BD Biosciences, CA, USA).
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PMC10213179
Cells were treated with 100 nM of ATOP or SCR every 72 h for 15 days and their capacity to form clones was evaluated. (
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PMC11222184
Debio0932 did not induce alterations in the expression of genes associated with cellular senescence.
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PMC9429973
Dividing evaluable patients in either standard risk (absence of any high-risk aberration; 320/578, 55.4%) vs. high-risk (exactly one high-risk aberration; 176/578, 30.4%) vs. ultra high-risk (≥2 high-risk aberrations; 82/578, 14.2%) yielded similar efficacy results.
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PMC5035325
The N-Acetylneuramic acid (NeuAcGM3) ganglioside, but not the N-glycolylneuramic acid (NeuGcGM3), is usually detected in normal human tissues.
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PMC11696576
The XI(50) value and stdev values reported are for the entire curve.
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PMC11743316
Activated PI3K converts PIP2 to PIP3, which subsequently mediates the phosphorylation of Akt through PDK1.
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PMC11564322
The increase in the ELANE mRNA expression noted in our experiments may also suggest an increase in the migration of the tested cells.
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PMC11806818
Traditional separation methods usually include density gradient centrifugation, filtration, and immunomagnetic separation .
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PMC9672323
Two human glioblastoma cell lines, U-87 MG (Figure 1sa) and A-172 (Figure 1sb) cells were obtained from the national cell bank of Iran (Pasteur Institute, Tehran, Iran) and cultured in RPMI-1640 medium (Capricorn Scientific, Ebsdorfergrund, Germany) supplemented with 10% heat-inactivated fetal bovine serum (FBS), 100 U...
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PMC10196713
It has been reported that pretreatment with erastin, an inducer of ferroptosis, enhances the therapeutic effect of cisplatin (20), and that concomitant overexpression of ferroptosis suppressors, SLC7A11 and GPX4, can often be observed in platinum-resistant OC (21).
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PMC11721587
As shown in fig. S5C, Fu condensates were resistant to 1% Triton X-100 or 4 M urea, suggesting that Fu condensation was not due to its membrane association or forming prion-like protein aggregates.
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PMC9429973
In the non–high-risk group, the VAS and FACT-Lym total scores remained stable or improved except later cycles with small sample sizes.
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PMC11422150
The bone marrow was washed, re-suspended in 25% Percoll (GE Healthcare), and centrifuged for 20 min at 500 g. The supernatants were discarded.
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PMC4816271
Overall the Fc spacer provided the optimal response whilst both receptor variants containing the CD8 stalk appeared sub-optimal in terms of cytotoxicity and IL-2 release, although the stalk-variant-transduced PBMCs were consistently observed to secrete higher levels of IFN-γ upon stimulation than PBMCs transduced with ...
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PMC11297139
Reverse-crosslinking was performed by incubating the eluted DNA in 65 °C overnight.
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PMC11594641
Our findings suggest that the involvement of Rab27A is cell-line-dependent, rather than resulting from abundant protein levels.
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PMC9429973
Methods: he authors reviewed 541 patients who were diagnosed with DLBCL treated with R-CHOP (rituximab with cyclophosphamide, hydroxydaunorubicin, vincristine, and prednisone,R-CHOP) in 3 years.
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PMC9429973
No new unexpected safety signals have been identified.
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PMC9429973
The risk of CHF was more pronounced in HDT-treated males compared to females (10-year incidence 7.8% vs 3.8%, respectively, Fig. 1c and Fig. 1d); however, the risk was significantly increased for both sexes (p<0.001 for both).
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PMC11683240
This allowed us to establish a hierarchical order from the most stable complexes to the lower, based on the binding energy obtained with this server.
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PMC5673955
N = 4. *
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PMC6182045
These fractions were further purified via size exclusion chromatography using a 60 cm Highload Superdex 200pg column (GE Healthcare) in pH 8 TBS (Tris-buffered Saline) buffer.
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PMC9318744
Commercial cell lines A549-hACE2 (a549-hace2, lot 42-01) and A549-hACE2-TMPRSS2 (a549-hace2tpsa, lot 42-02) were purchased from InvivoGen in February 2021.
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PMC11320834
Samples were analyzed by HPLC with a UV/vis detector using an XTerra C18 Column, 125 Å, 5 µm, 4.6 mm × 150 mm (Waters Corporation, Milford, MA, USA) operated at 25 °C.
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PMC11772449
BEL-7404, Huh-7 and PLC/PRF/5 were treated with DMSO, Sora (1.25/2.5/5μM), PFD (1/2mM) and their combination, respectively.
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PMC11740573
The aim of this preliminary study was to evaluate in vitro the influence of calcitriol on the immune response of human vaginal epithelial cells to bacterial infection.
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PMC11549062
Western blot analyses of Cleaved Caspase‐3 of cos7 cells treated with knockdown or overexpression of PTP1B and Rab5, respectively. (
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PMC9429973
E. Genovese, M. Mirabile, S. Rontauroli, S. Sartini, S. Fantini, L. Tavernari, M. Maccaferri, P. Guglielmelli, E. Bianchi, S. Parenti, C. Carretta, S. Mallia, S. Castellano, C. Colasante, M. Balliu, N. Bartalucci, R. Palmieri, T. Ottone, B. Mora, L. Potenza, F. Passamonti, M. T. Voso, M. Luppi, A. M. Vannucchi, E. Tagl...
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PMC3765139
Final amounts of target were determined as follows: target amount = 2, where Ct = [Ct (MGP D10 CD133+) – Ct (ACTB)]sample – [Ct (MGP D10 CD133-) – Ct (ACTB)]calibrator.
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PMC11707577
Brightfield (left column), fluorescence (middle), and merged (right column) images of a representative Hydra polyp treated 30 min with the M13EGFR(TNP) and irradiated for 10 min.
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