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PMC10142392
Through a three-step reaction sequence , a multi-functional initiator has been synthesized which has one initiation site for ROP and two initiation sites for ATRP.
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PMC11087055
This study suggests that amniote gastrulation may be evolutionarily distinct from non-amniote gastrulation in the mechanisms coupling large-scale cell flow and midline morphogenesis.
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PMC11476837
Doxorubicin significantly increased ROS level at the concentrations of 10 and 100 nM in HL-60 cells (p < 0.001) and was significantly higher than in HL-60/DR cells (p < 0.001).
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PMC9024365
Membranes were washed with TBST and incubated in HRP conjugated goat anti-rat and HRP-conjugated goat anti-mouse secondary antibodies for 1 h at room temperature.
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PMC7504302
The primary aim is to identify new risk factors and novel therapeutic targets and treatment strategies in MPM, and integrative and high throughput genomics and functional genomics studies can definitely help advance these efforts.
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PMC8728081
The cells were incubated in 5% CO2 atmosphere at 37 °C.
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PMC9429973
This suggests that T-cell priming capacity mediated by the interaction with pDCs is conserved through TLR7 activation.
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PMC11763126
Fig. 11Schematic diagram illustrating how CBDP1 inhibits ccRCC progression by regulating the USP5/YTHDF2/TRPM3 axis Analysis of peptides differentially expressed in ccRCC.
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PMC10329237
BLM has been widely used in chemotherapy for many years (17).
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PMC11767725
Its pathogenesis is driven by hyperseborrhea, follicular hyperkeratinization, microbial dysbiosis—particularly involving C. acnes—and inflammation.
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PMC11766174
The specificity and sensitivity of CA-ICC were assessed by staining a cell line that expresses CA (MOLT IIIB) alongside a control cell line (Jurkat) devoid of this marker, as well as peripheral blood mononuclear cells (PBMCs) from HIV seronegative donors before or after ex vivo infection with an HIV laboratory strain.
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PMC10671321
For the western blot, protein extracts were denatured in Laemmli buffer (BioRad, South Granville, NSW, Australia) and β-mercaptoethanol at 95 °C for 10 min; then, they were resolved on 8% Bis tris polyacrylamide gel (Thermo Scientific, Waltham, MA, USA) in MOPS SDS running buffer (Thermo scientific, Waltham, MA, USA) a...
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PMC11786600
Effect of varying portable electrospinning dressings on wound healing. (
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PMC5025404
Gene sequences were obtained from publicly available sources (DrugBank and patent databases).
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PMC10142392
These micelles, which measure approximately 100 nm, were obtained from a diblock copolymer of PEG and PCL linked by a pH-sensitive hydrazine (Hyd) bond, using a solvent evaporation method.
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PMC11089031
However, we found that the high expression level G3BP2 was associated with better prognostic in ccRCC.
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PMC11352877
With the progression of disease and changes in the demands that cells make of the blood supply, this ratio can shift.
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PMC10170482
Immunoblot analysis revealed that culturing HepG2 cells in soft 3D CS conditions leads to a marked decrease of Bcl-2 expression level (Figure 6A).
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PMC4369959
The human GI tract is a single tube which is approximately nine meters long in relaxed condition .
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PMC10419319
Gibco (Grand Island, NY, USA) supplied RPMI-1640 medium, fetal bovine serum (FBS), and Dulbecco’s phosphate-buffered saline (DPBS).
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PMC11365983
Western immunoblots of meningeal tissue lysates reveal similar reductions in total Pdgfrα levels to those observed by immunofluorescence (replicates as in Fig. 7). [
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PMC8010066
The Relative Pyoverdine Production (RPP) was calculated using RPP: OD405/OD600 (Dehbashi et al. 2020).
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PMC9429973
Serum LDH was assessed.
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PMC11423992
TFAB002s caused a cross-linking interaction between WIL2-S and CD3 T cells, leading to the lysis of WIL2-S cells (Fig 3B).
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PMC9429973
Median age was 62 years old (range 27-91 years), 216 pts (51%) were male.
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PMC9429973
d1–14 and d22-35; bortezomib 1.3 mg/m s.c.
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PMC9429973
Kaplan Meier system was used to estimate survival.
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PMC9429973
Results: The sample included 50 patients: 11 with HH (100% MM) and 39 NHH (87.18% MM, 12.82% AL).
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PMC6835428
PLGA, biodegradable into its original monomers LA and GA, has been approved for human use by the US Food and Drug administration.
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PMC10850553
The alternation in the coagulation-fibrinolysis system in IA lesions of rats.
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PMC11757230
1Inhibitory effect of canine adipose-derived mesenchymal stromal cells (cADSCs) on the proliferation of hematologic malignancy cells in vitro. (
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PMC11754291
Assessment of Cell Viability Using the AlamarBlue HS Assay.
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PMC11036064
First, we could not include in-vivo and ex-vivo studies.
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PMC10847511
The drugs were divided into left and right panels based on positive and negative median differences, and further ordered based on the lowest and highest p-values (Mann-Whitney test), respectively.
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PMC11680049
When cultivating primary explants (shoot segments) horizontally in light, callus formation was inhibited, and only in rare cases on days 30 to 40 was dark brown callus with green inclusions observed (Figure 3c,d).
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PMC8316344
We found a significant cell death in all the tested liver cancer cell lines following treating with either RSL3 or erastin for 12 h, and such effects could be prevented by simultaneously treating with iron chelator DFO (Fig. 1A), suggesting ferroptosis did occur.
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PMC11547917
The IC50 values obtained by the MTT cytotoxicity assay represent a weak response of the extract on the Caco-2 cells at two timeframes.
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PMC9429973
The diagnosis was made on 2 different sites in 11 patients (55% of cases).
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PMC10366908
The KD values of EMab-300 for CHO/mEGFR and NMuMG were determined as 4.3 × 10 M and 1.9 × 10 M, respectively (Figure 3A,B).
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PMC3888431
A graphical representation of the reference-based transcripts and their intron-exon structures on the CHO-K1 draft genome is publicly available at the GenDBE web-interface (https://gendbe.cebitec.uni-bielefeld.de/cho.html) (Figure 10).
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PMC10123657
In that work, the dissociation was made using 70 μm strainers, a mechanical dissociator, or by flushing the BM through a 27G needle prior to filtration (40-micrometers) .
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PMC10729469
Mice were allowed to explore the maze freely for 8 min, and the total entries were recorded visually.
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PMC11365427
The authors declare no conflict of interest.
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PMC10900886
Coxsackievirus type B infects murine splenic B cells resulting in production of new virus particles (21), and poliovirus productively infects DCs and macrophages in vitro (human PBMC) and in vivo (non-human primates) (22, 23).
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PMC11545737
The cytotoxin-associated gene pathogenicity island (cagPAI) is critical in the ability of H. pylori to induce pyroptosis.
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PMC10530622
The studies that focused on microbiota have mainly explored the microbial composition, and bacteria in particular.
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PMC8345486
The control cells were cultured in a basal medium and a medium supplemented with a vehicle (DMF, 0.02%, not toxic).
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PMC11687663
The software explored potential binding conformations, predicting the binding affinity of each ligand with the protein.
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PMC9429973
Methods: This study was a multi-national, open-label study with romiplostim in IST-naïve adult patients with AA (NCT03957694).
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PMC11307990
786-0 cells (1.5 × 10 in 1 mL of medium) were seeded in a 96-well plate and cultured overnight.
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PMC10093184
Pictures were taken using a light microscope (Zeiss Observer Z1, Oberkochen, Germany).
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PMC11389534
For instance, the UFM1 system contributed to the processes in maintaining ER homeostasis, including ER‐ribosome‐associated quality control (ER‐RQC) , , , , , , and ER‐phagy. , , ,
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PMC11474209
The mtDNA is vulnerable to oxygen-free radicals and stress-induced injury as it lacks histones in the structure and efficient repair mechanisms.
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PMC11597291
The colloidal stability of GO nanomaterials in physiological media is enhanced by the covalent attachment of hydrophilic polymers, such as PEG , poly(lactide)-poly(ethylene glycol) copolymers , dextran , pluronics , and tocopherol polyethylene glycol 1000 succinate (TPGS) .
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PMC10729469
Lonza, Basel, Switzerland) and electroporated using the DN-100 program as previously described.
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PMC10858941
Specifically, less than 10% of such drugs are ultimately commercialised, with a substantial proportion failing during the final stages of clinical development despite obtaining promising results in preclinical models .
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PMC8916024
Our PCoA results confirmed clustering of these two groups, which indicated that the intestinal flora of C. chinensis was relatively stable during the growth process.
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PMC10452486
Similarly, Antoniou et al. suggest the possibility of HC dimerization through bonding between C of one HC with C of another HC.
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PMC10671321
Peripheral blood Mononuclear Cell were purified from a blood sample by Ficoll gradient at the Genethon (Évry, France).
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PMC11628904
All larvae were burned in control medium, and treatment with isotonic medium began 1 hpb.
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PMC8358006
In contrast, the broadest domains of other histone marks of H3K4me3, H3K4me1, and H3K27ac were not specifically enriched for epithelium development (Supplementary Fig. 1).
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PMC11257203
Table 1 shows that the average ratio of PCE to NCE in the indomethacin-treated groups at 20 and 40 mg/kg showed a significant decrease compared to the control group.
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PMC3734024
IL-10 in the culture supernatants was measured by ELISA. (
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PMC11467800
To this purpose, we outcrossed adult Casper mutants to the Tg(fli1:EGFP) transgenic strain of zebrafish and collected the embryos.
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PMC9275501
In vivo experiments showed that compared with (DOX)-micelles, (CUR)-micelles, free (DOX + CUR), free DOX, and free CUR, (DOX + CUR)-micelles had more tumor accumulation and better antitumor effect in drug-resistant MCF-7/ADR xenograft model.
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PMC8073654
Thus, 4 exhibited an apoptogenic property in the CEMss cell line via the intrinsic mitochondrial pathway of apoptosis induction.
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PMC10142392
Polymer chemistry specialists have developed methods to design multi-branched polymers that can be used for targeted drug delivery, imaging, and even precision medicine.
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PMC8336407
The P. biossieriana Buhse leaf extract at concentrations of 1.5 and 2.0 mg/mL is capable of attenuating t-BHP-induced cytotoxicity in HepG2 cells.
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PMC10547921
However, diphosphate esters in the CDN structure will cause low bioavailability of drugs.
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PMC9429973
Screening was done utilizing a complete iron profile with complete blood count and the Mentzer index, defined as the mean corpuscular volume divided by the red blood cell count.
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PMC3035438
Expression and diversification of chicken IgM and of chimeric IgG in transformed DT40 cells. (
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PMC10291556
The “shake flask” method was used to measure the partition coefficients (P) of platinum compounds.
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PMC11632064
Two additional selective RSK inhibitors, BIX02565 (Edgar et al, 2014) and LJI308 (Aronchik et al, 2014), had no effect on MeWo cells but had strong dose-dependent anti-inflammatory effects in A-375 cells (Fig 1G and H).
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PMC9429973
More clinical data will be updated from this ongoing study.
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PMC11585994
Hematologic malignancies have traditionally relied on chemotherapy for long-term management.
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PMC10502403
These results support the enhanced antitumor activity of αβ-TCR and CD8 gene-cotransduced γδ-T cells.
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PMC11087766
Whole genome sequencing was performed by SeqCenter (Pittsburgh) using Illumina NextSeq 2000 at a depth of 200Mbp.
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PMC11638764
Proteins without error bars indicate that only a single abundance value was determined.(I-K) Pathway analysis of significantly downregulated proteins in CER (I), NER(J), and INS(K).
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PMC11306019
Data represent the mean ± SD (n = 3).
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PMC9429973
Time zero (T0) was the date when the first subsequent therapy started after patient met inclusion criteria.
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PMC11126803
The antagonist of ERβ, ICI 182,780, may benefit patient survival by inhibiting the expression of ERβ and thus blocking the transmission of this signaling axis.
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PMC11803918
This has inspired us to explore further cell-free therapy, i.e., extracellular vesicles derived from γδ-T cells, to expand their clinical applications and improve the therapeutic efficacy for solid cancer treatment.
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PMC8345486
Intrinsic drug resistance and acquired drug resistance are major causes of failure of therapy for ovarian cancer, the most deadly gynecologic malignancy .
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PMC11200978
The addition of gemcitabine pre-treatment increased the lethal temperature to −20 °C for a single 5 min exposure and −15 °C for a repeat exposure, representing a significant improvement in the likelihood of complete cancer destruction.
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PMC9428827
MB16, CT26, and TC-1 cells were inoculated s.c.
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PMC11588008
Cell count: If the cell density is high, the cells can be diluted 10 times first, and then 10 µL of cell suspension can be added to the cell culture plate for counting.
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PMC11377827
CA9: control vs. HIF1β sgRNA ***P = 0.0001, control vs. USP43 sgRNA **P = 0.0036.
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PMC11261875
The intertumor heterogeneity in the metabolic phenotype of imatinib-resistant GIST needs to further investigated.
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PMC11583010
The affinity of the 15A7.5, 5A12.2, and HA22 mAbs was measured by biolayer interferometry technology and indicated a ten-fold lower affinity of 15A7.5 versus 5A12.2 and HA22 toward a monomeric recombinant His-tagged protein corresponding to the full extracellular domain of human nectin-4 (Supplementary Table S3).
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PMC10854447
The m-halophenyl moiety stacked with Phe699 deeply inside the hydrophobic cleft of the enzyme and formed a halogen bond interaction with Leu764 in the case of compound 7i with (R = m-Br).
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PMC10605143
HSF1 is a ubiquitously expressed transcription factor.
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PMC9429973
Results: Three hundred and sixteen CMV positive patients were included (186 in P1, 130 in P2).
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PMC9370419
The extracted RNA was separated by denaturing 1.5% formaldehyde agarose gel electrophoresis and transferred to a Hybong-Nþ membrane (Amersham Biosciences, Amersham, UK) in 20 SSC (Invitrogen, Carlsbad, CA, USA).
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PMC11634027
The ability to associate with MyoID was conserved in the DCHS1 ICD (Fig. 8M).
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PMC11409031
Subsequent to washing, observations of the samples were made using an Olympus inverted microscope.
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PMC10158546
Upon growth factor removal, ReN progenitor cells lose their ability to proliferate and instead differentiate into glial and neuronal cell types, coinciding with morphological changes as seen in the cultures [29–32].
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PMC10033453
BALB/c mice were inoculated subcutaneously with WEHI 164.
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PMC10874773
They are mainly distributed in I. majus and I. jiadifengpi.
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PMC9429973
Treatment with ibrutinib may overcome T-cell dysfunction by not only controlling leukemic cells but also promoting antitumoral T-cell responses mediated by promoting Th1 polarization, restoring CD4: B-cell interactions, and inducing CD8 T-cells cytotoxicity.
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PMC10728200
Our results suggest that IM may promote ferroptosis by regulating STUB1 expression in GIST.Fig.
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