PMCID
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PMC11695546
Following the discarding of the supernatant, each well's free-floating cells were thoroughly cleaned using sterile distilled water.
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PMC6642070
Furthermore, lovastatin treatment neither changed the body weight significantly nor caused any obvious physical/health problems of the mice.
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PMC10571053
Thus, our findings will be valuable as these compounds are pursued clinically.
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PMC9429973
We are currently analysing scRNA-seq data from bone marrow hematopoietic and niche cells to dissect the molecular differences in steady state hematopoiesis of mice subject to horizontal versus vertical transfer of pathogens/microbiota, to better understand why the HSC function might be compromised in co-housed mice but...
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PMC11746942
It also suppressed keratinocyte hyperproliferation, as evidenced by reduced expression of Ki67, a marker of cellular proliferation (Fig. 1D, E).
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PMC11541409
Another study reported that 882R (IC50 5 µM) cells exhibited higher glucose uptake, increased expression of LDHA and oxidative phosphorylation (OXPHOS) protein compared with GIST 882 cells.
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PMC11547972
Anti-PTEN (clone 26H9) was from Cell Signaling (Leiden, Netherlands, anti-pCDK2 (Thr160) from Thermo Fisher Scientific, Schwerte, Germany, and anti-histone H3 (IgG, clone 3H1), anti-acetylated H3 (aH3; IgG, Lys9, clone C5B11), and anti-histone H4 (IgG, clone L64C1,) were all from Cell Signaling (Leiden, The Netherlands...
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PMC9261184
Earlier generations of LNG carriers, designed and constructed using older technologies for storage and BOG management, may exhibit varying emissions profiles compared to this ship.
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PMC9878562
VAL-wt, which heterogeneously express CCR7 ( Figure 1A ), were sorted for CCR7 surface expression and tested separately.
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PMC10317042
The relatively small change of ATAC-seq in the EWSR1 promoter region compared with that of H3K27ac and H3K9ac prompted us to explore other mechanisms of transcriptional regulation through histone acetylation.
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PMC11748363
Sixteen out of a total of 33 compounds had activity against Mtb H37Ra (strain of main interest) equal to or below 31.25 μg/mL (cutoff value for activity).
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PMC9014716
An in vivo experiment was conducted to verify the ability of PS-T to increase the sensitivity of human Bel-7404 and HepG2 cells to OXA.
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PMC9429973
Two patients had 2 distinct PPGV.
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PMC7039683
A role for Tead family members in periderm development is supported by the observation that a loss of function mutation in yap, encoding a cofactor of Tead, disrupts periderm development in medaka (Porazinski et al., 2015).
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PMC11730311
First, a series of lipidated hαCGRP8-37 analogues were screened for their antagonism against the hCGRP-R. Next, a subset of hαCGRP8-37 analogues conjugated with C20DA-γGlu at varying positions was analysed for in vivo exposure in mice.
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PMC11335267
Lack of expression of Alx3 in the spinal cord of wild type mice.
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PMC11725127
Apoptotic cells were detected in sections using the TUNEL staining.
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PMC11310713
Next, we confirmed the sustained release of ovarian sex steroid hormones (E2 and P4) from the fabricated GTBs over a duration corresponding to 2 menstrual cycles in humans (Fig. 2E and F).
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PMC11794847
In addition, the CMT-64 murine lung cancer cell was also implanted into HVEM KO and wild type mice subcutaneously.
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PMC11122631
An additional acetoxy group (2″-OAc) was assigned on C-12 due to its downfield-shifted chemical shift and considering the molecular formula.
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PMC7215722
Next, we asked whether these effects are not restricted to SI but may also affect other glycoproteins that are trafficked along the constitutive secretory pathway.
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PMC9351137
Images were imported into ImageJ software (http://rsbweb.nih.gov/ij/) and adjusted for brightness and contrast uniformly across entire fields where appropriate.
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PMC10654497
Kaplan–Meier analysis demonstrating the prognostic significance of the risk model in (D–G) TCGA and (D) GSE37745 cohorts.
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PMC9874064
The establishment of a cisplatin resistant liver cancer mice model, and the schedule of drug administration for the anti-tumor efficacy study of NP-Pt-USP1i in vivo.
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PMC11725127
Specifically, we demonstrate that gambogic acid (GA) forms a covalent bond with the cysteine 284 residue of USP2 through an allosteric pocket, inhibiting its deubiquitinating activity.
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PMC11283030
Original magnification 200×; bars, 50 μm.
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PMC11754784
For microenvironment-confined DNA compution, there are mainly two kinds of leakages: 1) the leakage caused by cross talk between target cells and by-standing cells (type I leakage); 2) the leakage caused by cross talk between by-standing cells (type II leakage).
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PMC9622879
The optimization problem of the community was solved by the particle swarm optimization (PSO) algorithm and a day-ahead schedule of sharing the DERs was provided.
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PMC11377827
Genomic DNA was extracted using a Gentra Puregene Core kit.
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PMC11790599
Additionally, the yield from plant extraction is also very low (Luo et al., 1986; Xu et al., 2006).
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PMC7185206
Box plots quantifying flow cytometry mean fluorescence intensity (MFI) shown, compared statistically by Student’s t test. (
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PMC11803149
Briefly, plasmid DNA was mixed with PEI at a ratio of DNA: PEI=1:5 in 2.5 mL of Opti-MEM (Invitrogen, 31985-062), vortexed, and incubated for 30 min at room temperature (RT).
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PMC11389534
Therapeutically, a covalent inhibitor of UFSP2, known as compound‐8, has been shown to promote PD‐L1 UFMylation and contribute to combination in vivo treatment with PD‐1 blockade.
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PMC10850553
Receptors mediating neutrophil recruitment could thus be therapeutic targets of unruptured IAs.
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PMC9118379
For its SAV S197R, the SAV-harboring protein contained in the customized database for database searches yields fully digested peptides 187-FFGSLPDSWAR-197 and 198-GHSVPVVTVVR-207, neither of which belong to the wild-type peptide space and thus are regarded as variant peptides by database search engines.
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PMC8998437
Although the vast majority of eBL tumors display latency I EBV program and express the EBNA1 protein only, some studies reported that BL biopsy samples and early-passage BL cell lines are heterogeneous and express additional EBV latent and lytic cycle genes .
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PMC9429973
Mechanisms of action of daratumumab were studied by flow cytometry (FCM) in MM cell lines pretreated with tinostamustine and cultured as specified: 1) with F(ab)2 fragments (direct apoptosis via crosslinking); 2) with 10% human serum (CDC assays); 3) co-cultures of MM and NK cells (ADCC assays).
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PMC10713411
In summary, the PRPCDGs risk signature was developed and showed stable prediction efficacy for the prognosis of patients and that (such as TRIB3) could be a potential target for RCC management.
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PMC11412752
Some studies suggest that STING may also play a role in controlling bacterial infections by modulating inflammation and immune responses .
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PMC11320084
TNNT1 mRNA (Figure 1d,e) and protein (Figure 1f,g) expression were markedly decreased after si‐TNNT1 transfection, suggesting si‐TNNT1 effectively reduced TNNT1 expression.
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PMC11753435
We generated plasmids expressing each of these IPs, fused to the UltraID DNA coding sequence via several flexible linkers and a hemagglutinin antibody (HA) epitope.
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PMC9429973
Methods: CLL cell lines (MEC-1, MEC-2 and PCL-12), patients peripheral blood and bone marrow mononuclear cells (PBMC and BMMC) and B lymphocytes purified from patients PB were treated with 300 µM ferric ammonium citrate (FeAC) alone or in combination with the BTK inhibitor ibrutinib (IBR, 5-10 µM) or the BCL2 inhibitor...
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PMC10291556
HPLC and NMR characterizations were consistent with what was expected and are provided in Figures S3–S8.
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PMC8193813
All sensors were selected for their applicability to the anticipated operating conditions of concentration level and temperature as well as for their ability to rapidly respond to changing plume concentrations due to turbulence and entrainment of ambient air.
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PMC11394227
The apoptosis rate of CHO-PAb and CHO-PAb-QS cells under puromycin treatment was 32.6% and 17.7%, respectively, and the apoptosis rate of CHO-PAb-QS cells was 45.7% lower than that of CHO-PAb cells.
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PMC4485648
pRL-TK Renilla vector (Promega) served as a transfection efficiency control.
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PMC8873393
Each bar represents the number of noncoding transcripts having a specific number of exons.
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PMC11767793
These commercial sources are referred to as C1 and C2 in no particular order.
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PMC11754291
While we observed robust evidence of melanogenesis, the study did not differentiate between eumelanin and pheomelanin.
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PMC6379402
For instance, cancer is not the consequence of a single mutated gene, or of a single-broken interaction, but a result of multiple perturbations within and across cells.
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PMC11697703
Nuclear MAPK8 regulates transcription factor activity and chromatin state.
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PMC4816271
A phase I clinical study of anti-GD2 CAR transduced T-cells in relapsed high risk neuroblastoma patients reported some efficacy .
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PMC10976516
injection with R-LM249-loaded FM-MSCs efficiently prevented metastatic tumor formation in the lungs of subcutaneous xenotransplanted human ovarian cancer cell line SK-OV-3.Leoni et al.Cf33-GFPChimeric poxvirus generated through homologous recombination among nine strains/species of poxviruses, expressing GFPIntratumora...
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PMC6379402
c Each line shows the fitting of a network model (color coded, Erdös–Rènyi (ER), generalized random (ER-DD), geometric (GEO), geometric with gene duplication (GEO-GD), scale-free (SF), scale-free with gene duplication and divergence (SF-GD), and stickiness-index based model (STICKY)) for the different types of real-wor...
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PMC11707577
Stained tentacles were observed under bright field and phase‐contrast microscopy (Axiovert 100, Zeiss) equipped with a Leica K5C digital microscope camera.
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PMC11377827
This strategy proved powerful in identifying USP43 and its involvement in HIF-1 activation through associating with 14-3-3ζ/ε.
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PMC9429973
Medical records relevant to prognosis were collected by a chart review.
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PMC6034753
During MDV-CAV co-infection also, there should be multiple molecular partners that would potentially interact with the Meq-apoptin complexes.
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PMC11805741
The exceptional antioxidant properties of carotenoids orchestrate their modulation of oxidative stress, which is considered the major cause of cancer prognosis.
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PMC11612794
Since CENP-T is not phosphorylated in interphase, S175 and T184 of CENP-T do not interact with the stalk basic surface of Mis12C.
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PMC11473750
The absence of deep pockets and the unstructured nature of the MYCN and MYC proteins has meant that designing direct drug inhibitors has been challenging.
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PMC11755519
EVs can also modulate the immune response by inhibiting T and NK cell activation, suppressing the activity of dendritic cells (DCs) and increasing the expansion of myeloid-derived suppressor cells and stimulating macrophage polarization toward the tumor-promoting M2 phenotype.
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PMC11706491
Boxplot represents quartile distribution.
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PMC9559528
A weakened humoral response and a relatively heightened CD8+ T-cell response result in prolonged infection and progression to advanced syphilitic stages, such as what was seen in this case .
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PMC10891340
Based on the above results we identified that CCCP had no effect on the life cycle of DPV.
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PMC11699042
i Left, collective variability in drug response of all A-673, HeLa, and MCF-7 cell line strains depicted as the standard error of ED, each compound is shown as a black circle, one-sided Wilcoxon signed-rank test.
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PMC11361748
Data are shown as mean ± SEM.
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PMC6600253
A well-known example of the evidence of OIS is the overexpression of RAS.
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PMC3675084
The paclitaxel-resistant OVCAR-3-TR and SKOV-3-TR sublines showed overexpression of P-gp (Figure 2A), an efflux pump able to hamper retention of taxanes and other cationic drugs .
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PMC6835762
The chemo- and regioselectivity of lipase allows the hydroxyl moiety of the PGA backbone to remain intact, without the need for tedious and complicated protection and deprotection steps .
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PMC10999934
The expression level of FBXO2 among treated with cisplatin and untreated with cisplatin group in A2780 cell line, IGROV-1 cell line and OVCA429 cell line. (
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PMC10443665
And where cells were treated with bassorin, DMSO (0.5% v/v) was also added to the wells.
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PMC11593084
The complexes mediate the formation of tumor invadopodia, which promotes extracellular matrix (ECM) degradation and invasiveness .
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PMC9429973
AEs reported in >70% of pts were CRS (87%; grade [gr] 3/4 0%; gr 1 73%), neutropenia (97%; gr 3/4 100%), anemia (85%; gr 3/4 43%), and thrombocytopenia (79%; gr 3/4 70%).
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PMC10578720
This acetylation-mediated modulation might lead to impairment of the TCA cycle in macrophages.
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PMC11515150
Cytotoxic activity against CD5 hematologic malignant cell lines and normal T cells was exerted in vitro and NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt mouse model transplanted with Jurkat-Luc cells was used to evaluate the antitumor efficacy of CD5 CAR-NK cells in vivo.
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PMC8068766
The qualities of arrays were checked using simpleaffy package available in RMiller CJ (2020).
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PMC9429973
L. H. Sehn, K. Hübel, S. Luminari, A. Salar, B. E. Wahlin, A. K. Gopal, C. Bonnet, S. Paneesha, M. Trneny, H. Mashegu, C. Lihou, D. Li, C. W. Scholz BC Cancer Centre for Lymphoid Cancer and The University of British Columbia, Vancouver, Canada; Department of Internal Medicine I Oncology and Hematology, University Hospi...
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PMC10577955
It responded positively to Liebermann-Burchard test for triterpenes.
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PMC11271278
B Plotted are the calculated trans-enhancer contribution (x-axis) and the copy number estimated by SNP-array data (y-axis) for each HAPI gene in COLO320DM cells.
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PMC10936243
People are very concerned about cost.
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PMC11531735
Tumor-free survival of multiple treated groups were analyzed by Kaplan-Meier analysis employing the log-rank test with the Bonferroni correction using R Statistical Software.
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PMC9429973
Although the median PFS for Del17p subgroup on RVd-Elo arm is higher than RVd, it did not achieve statistical significance.
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PMC9429973
One patient had grade 1 ICANS that was fully resolved.
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PMC11615743
Recent advances in DNA logic devices offer a highly effective means for achieving one‐step identification of target cells with a high degree of efficiency.
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PMC11786767
Our Sholl analysis revealed that the number of intersections was greatly reduced in Ki-67 knockdown cells (Fig. 6C), that is, less than 50% of that enumerated for control cells (Fig. 6D).
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PMC11672881
Caspase-9, conversely, is involved in the intrinsic apoptotic pathway.
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PMC9681981
The results showed that the effects of bacterial harvesting time, cell treatment, and their interactions were not statistically significant on the mean expression of cpa gene (P<0.05); however, the effects of contact time and cell treatment are not shown here.
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PMC11066331
Heat maps of non-centered data (E) and median-centered data (F) were constructed from all peak intensities (feature matrix).
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PMC11462929
If the in vivo leukemic subgroup substantially harbors BENC‐CNA, which is beneficial for proliferation, ALL cell will predominantly relapse with BENC‐CNA.
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PMC7812570
As illustrated in Fig. 1A, the m36.4- or FluIgG03 scFv-encoding sequence was genetically fused with sequences encoding a His-tag and the GPI attachment signal of DAF and then inserted into a self-inactivating lentiviral vector (pRRLsin.
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PMC8143558
The results from the study suggested that Trichoderma spp were highly sensitive to A. minutiflorum Regel saponins.
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PMC11767725
Chronic inflammation can shift to mixed Th2/Th22 or Th1/Th17 responses, especially in severe or chronic lesions.
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PMC9516401
It could be postulated that these compounds could be used in the treatment of T2DM.
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PMC10988555
For this purpose, YSMs of chicken embryos were challenged with the IC50 concentrations of complexes 2–4 for 48 h. All newly formed vessels were counted at different time points after exposure (0 h (control), 24 h, and 48 h) and compared with the formed vessels for vehicle controls (DMSO 0.1%, Figure 16).
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PMC11488203
n = 3) whereby the numbers above brackets of the bars were calculated as fold induction of SDF-1 in taxol exosomes when compared to sdf-1 level in control exosomes (A).
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PMC9429973
We are currently in the process of validating prioritized target genes using a variety of approaches.
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PMC9275501
This dual strategy could control drug release in stem cells, reduce stem cell-related drug resistance, and enhance chemotherapy response.
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PMC9429973
As of July 2, 2021, 31 patients had been infused with C-CAR088.
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PMC11720808
The structure of SCU. (
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