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PMC11683240
These results suggest that the anti-protease activity of α1AT, as well as its potential inhibition of furin, may play a negligible role in our observations.
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PMC11701801
Treatment of cells with CBP/p300 dual inhibitors CP-C27 and A-485 downregulated expression of KREMEN2 mRNA in SMARCA4/SMARCA2-deficient cells (DMS114 and TOV112D) and SS18–SSX fusion cells (Fuji and Aska-SS; Fig. 4A; Supplementary Fig. S4A).
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PMC7812570
The human CD4 T cell line CEM-SS expressing CCR5 (CEMss-CCR5) was kindly gifted by Paul Zhou at the Institute Pasteur of Shanghai, Chinese Academy of Sciences, China.
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PMC11525028
Adequate Asn supports protein and nucleotide synthesis in tumor cells and facilitates osteosarcoma progression.
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PMC9429973
We used flow cytometry to detect apoptosis after cells were stained with Annexin V-FITC/Propidium Iodide (PI), and western blot to detect the expression levels of proteins.
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PMC11377827
HeLa cells stably expressing HA-USP43 or HA-UBEG2 were immunoprecipitated and immunoblotted using a pan 14-3-3 antibody (recognises all isoforms). (
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PMC7189327
The GO functional analysis showed that several genes, including CYFIP2, HOXB5, EGFR, FOXN3, PTPN3, CDC20, MARCKSL1, FAS, and PTCH1, primarily played pivotal roles in cellular processes.
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PMC9429973
The 3D model will be useful to highlight mechanisms behind drug resistance and to test patient-specific therapies.
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PMC11731161
In addition, we examined the effects of SEL on cell apoptosis in these cell lines.
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PMC11352311
Similarly, we applied network pharmacology to predict the potential mechanism of esculin in the treatment of RCC.
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PMC11291697
Birth weight was 3400 g. Early motor milestones were delayed: she sat at the age of 12 months and reached walking autonomy at 24 months, with frequent loss of balance.
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PMC11521786
Mice immunized as described above were challenged intravenously with 50,000 3LL-R3 cells.
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PMC11541409
In clinical trials, patients with PDGFRA are resistant to adjuvant imatinib therapy .
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PMC11496728
The cell pellet was collected and resuspended with 500 µL of phosphate-buffered saline (PBS), and the mean fluorescence intensity (MFI) of each eGFP was detected by flow cytometry.
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PMC11591038
We have shown before that the signal transduction pathways involving the inhibitor of DNA-binding/differentiation protein-1 (Id1), HIF-1α, cyclin-dependent kinases-5 (CDK5), sonic hedgehog, and protein kinase C-delta (PKCδ) may contribute to Aβ-induced cell cycle reentry and neurotoxicity in fully differentiated postmi...
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PMC11188874
The length of eluted peptides was not altered by HDAC6 treatment (Fig. 3A; Supplementary Fig. S13).
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PMC11566417
Antimicrobial assays were performed in triplicate, with four technical replicates averaged per assay, and a two-way ANOVA was performed.
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PMC10944868
Another important structural point to note in these compounds is also the R substituent, more interesting those with low number of carbon atoms, in particular the most active ones with methyl group.
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PMC9429973
In pts with del(17p), median PFS was NR (acala) vs 13.8 mo (IdR/BR; P<0.0001).
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PMC9592219
Cancer tissues had higher TOP2A mRNA and protein expression levels than the adjacent nontumor tissues (ANT), as shown on qRT-PCR and western blot analysis (Figures 2(c) and 2(d)).
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PMC11391695
The sections were subsequently captured on film using an inverted microscope.
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PMC5673955
to 1224 ± 687 b.p.
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PMC11300085
We infer that doses, exposure time of chemerin, or difference in type of ovarian cancer cells might account for its variation in biological function.
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PMC11551844
The procedures described by Ramanjooloo et al. (2023) were used.
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PMC7504302
Further studies are needed to confirm or refute a similar biological function in MPM biology.
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PMC11144200
The chemotherapeutic drugs and CTLA-4 blockers entering tumors lack targeting. (
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PMC11673902
Over the past two decades, many research tools based on microfluidic methods have been developed to measure the biophysical properties of single cells; however, it has still not been possible to develop a technique that allows for high-throughput, easy-to-operate and precise measurements of single-cell biophysical prop...
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PMC11530949
Also, we explored the cytotoxic impacts of the chemical synthesized Ag-doped CuO NPs and cisplatin on HepG2 cancer cells (Figure 4A).
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PMC11672142
Thus, it is reasonable that in interaction with DefNEtTrp, Fe(III) would preferentially bind to the Def moiety.
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PMC9693627
The peptides were eluted with a gradient of buffer B (80% acetonitrile, 0.1% formic acid) at a flow rate of 0.3 μL/min.
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PMC11615101
The confocal microscopy image shows TLR-2 expression (yellow) and nucleus (blue) in RAFLS. (
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PMC11701801
Mutation, CN, and gene expression datasets were obtained from the CCLE database and downloaded from the DepMap website [RRID: SCR_017655; data version 23Q2, (http://www.depmap.org/)].
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PMC9503669
Finding a cure for HIV-1 infection is therefore an essential goal of current AIDS research.
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PMC11593713
The method is based on changes in the concentration of NADPH that reacts with oxidized glutathione (GSSG) to form GSH.
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PMC11737091
In addition, it was determined whether DBF4 expression levels are correlated with different characteristics.
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PMC10810426
and E) PLA was performed using antibodies to MLV NC (p10) (D) or RT (E) and S9.6.
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PMC6379402
In the Human Protein Atlas, a gene is either expressed or not in a tissue according to antibody staining experiments.
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PMC7519871
The docking results of both BA and WDL showed satisfactory binding affinities.
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PMC8753818
The higher JRed expression (1.5-fold) in CHO-KO cells compared to CHO-K1 cells also confirmed the primary findings.
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PMC11770130
Ceramide is composed of one chain with stable length with hydroxyl groups and the other chain with variable length (the acyl chain synthesized by the CerS).
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PMC5363531
In advanced disease, chemotherapy as single agent (PEM) or in doublet combinations (PEM, cisplatin), despite inducing tumor shrinkage and improving overall survival, has mainly a palliative role with a median survival in advanced disease ranging between 9 and 17 months .
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PMC9429973
V. Musteata Discipline of Hematology, State University of Medicine and Pharmacy “N. Testemitanu”, Institute of Oncology, Chisinau, Moldova Background: The recent published reports pointed to the increased incidence of COVID-19 in patients with hematologic neoplasms as compared to the apparently healthy population.
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PMC11687167
Patients at risk of or with ASCVD Ref Reference, LDL-C Low-density lipoprotein cholesterol, LDLR Low-density lipoprotein receptor, siRNA Small interfering RNA, ASCVD Atherosclerotic cardiovascular disease, AMI Acute myocardial infarction, FH Familial hypercholesterolemia, L-IFPTA Liposomal Immunogenic Fused PCSK9-Tetan...
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PMC11661024
The IC50 value of two postbiotics for HeLa cell lines after 24 h was 10 µg/mL.Fig.
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PMC9429973
AIHA management during pregnancy and lactation is not standardized and drug use is often limited by safety concerns.
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PMC11599565
The expression of Stathmin is upregulated during neuronal development and has been associated with carcinogenesis and metastatic processes.
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PMC9429973
On the other hand, combination with bexmarilimab overcame the HLA-DR suppressing effect of venetoclax.
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PMC11767793
This could be attributed to some type of chemical transformation of PEI during the reduction for the Au-PEI@NIST preparation, as proposed in previous reports .
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PMC11765988
In sarcoidosis, microRNAs regulate the TGFβ and “wingless and integrase-1” pathways .
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PMC10490530
The importance of multiplexed BL assays to study simultaneous biological events from living systems lies in the undeniable potential to improve assessment reproducibility while minimizing the required number of replicates.
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PMC9848491
Kaplan-meier analysis was used to determine the OS time in the high-risk and low-risk groups to assess the value of the risk score in predicting the prognosis of UCEC patients.
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PMC11467964
For instance, experiments such as Western blot or immunohistochemistry are important methods to further validate these results.
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PMC10587429
For cancer types in which T or B cells play more critical roles, the model needs to be modified to support more robust reconstitution of these immune cells.
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PMC11267830
The 150 bp paired-end reads were aligned to the GENCODE v29 gene reference and the hg38 genome using STAR software (version 2.7.10a) with the parameters --outFilterMultimapNmax 1 --outFilterMismatchNoverReadLmax 0.04 --alignEndsType EndToEnd.
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PMC9429973
Patients complained of hemorrhagic manifestations.
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PMC10998613
These results suggest that epinephrine significantly increased the degree of multimerization between CXCR4 and β2AR in the DKO MDA-MB-231 cells.
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PMC11388384
The simulation continued until the flux reached a constant rate (40,000 time steps representing 240 s of simulated time), and the effective flux (Jeff) was calculated as the steady state slope of the number of secreted molecules as a function of time under each molecular transition rate.
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PMC9504875
Only TRIM25 was co-precipitated with N protein (Figure 2A, lanes 7 and 8), while none of the RLRs including RIG-I, LGP2, and MDA5, were co-precipitated with N protein (Figure 2A, lanes 1–6).
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PMC9352806
AnimalsMedianLifespan (days)Mean lifespan (days±SE)MaximumLifespan (days)P-valueN201261817.07 ± 0.3524＜0.0011001082019.91 ± 0.36262001382019.43 ± 0.3526＜0.001daf-16016088.81± 0.31810014467.13± 0.218<0.0001hlh-3001601211.71±0.46261001291212.14±0.47260.8026hlh-30 (RNAi)01102018.02±0.5261001091414.99±0.57260.0009Worms wer...
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PMC2614416
Loredana and co-workers profiled the proteome of normal human breast tissues, and compared these with those of the corresponding 8701-BC breast cancer cell line (ductal infiltrating carcinoma, DIC).
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PMC7961460
In MCF-7 cells, an autocrine effect of c10orf118 on HAS2, its antisense and CD44 was found.
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PMC9429973
Captivatingly, Sil or CK2 gene silencing led to a substantial reduction of the Ven-induced increase of MCL-1, potentially counteracting a deleterious Ven-induced drawback.
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PMC7802142
Together, these results indicate that ReN-NVs have anagen-promoting activity of the HF cycle via the regulation of β-catenin signaling pathway.
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PMC11489175
In ovarian cancer, the interferon (IFN) pathway has been found to be enriched in samples with low USP35, suggesting a role of USP35 in preventing recruitment of immune cells 98.
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PMC11763126
A Colony formation assay showing RCC cell proliferation after different treatments.
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PMC9429973
The CT genotype at rs11549465 was significantly higher in ITP patients sensitive to glucocorticoid-treatment than in those insensitive to glucocorticoid-treatment (P = .025).
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PMC11777207
To further establish whether PD-1 contributes to regulating DC migration in our animal model, we treated mice with αPD-1 and assessed differences in DC migration (Fig. 3L and fig. S5A).
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PMC11705862
Western blots showing the effect of glucose and glucolipotoxicity on IRIS-2 expression, and (F) effects of glucolipotoxicity and pro-inflammatory cytokine addition on IRIS-2 expression, with quantifications shown in (G) and (H), respectively (n = 5).
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PMC9429973
MPN-SVT carries a significant mortality and morbidity burden and poses clinical management challenges.
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PMC10988555
The geometry was optimized without symmetry constraints.
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PMC3888431
Combining the results of both approaches, 65,561 transcripts were identified for CHO cell lines, which could be clustered by sequence identity into 17,598 gene clusters.
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PMC9250505
Briefly, cells plated on chamber slides were fixed with 4% paraformaldehyde (PFA) for 30 min.
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PMC9429973
As we demonstrated subclinical herpesviruses replication without concomitant clinical signs, indicating underestimation of the real frequency herpesviruses reactivation in NHL patients with COVID-19, complex diagnostic approach is required including DNA herpesviruses evaluating.
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PMC3122064
Function of p53 is regulated by several mechanisms, acting not only at the transcriptional and translational level, but also on stability, post-translational modification, and subcellular localisation of p53.
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PMC11791203
Further identification of the compound responsible for the cytotoxicity is required and the compound will serve as a drug candidate for the treatment of colorectal cancer.
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PMC11190538
The cells were treated with C-phycocyanin before, during, and after viral infection.
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PMC10747139
Cisplatin was used for comparison (IC50 = 6.7 ± 0.4 µM; 9.3 ± 0.8 µM; 5.7 ± 0.2 µM; 5.8 ± 0.5 µM; 15.2 ± 1.4 µM; and 19.6 ± 2.2 µM, respectively).
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PMC11290772
Consequently, it becomes challenging to establish a standardized criterion for selecting NK cells suitable for CAR transfection.
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PMC11720808
qRT-PCR was used to evaluate the mRNA levels of the indicated genes in OS cells treated with DMSO or SCU (100 μM) for 48 h. GAPDH served as the loading control. (
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PMC11727000
Precursor mass tolerance was 20 ppm for the first search where initial mass recalibration was completed, and then 4.5 ppm for the main search.
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PMC11748335
We also calculated the proportion of poised promoters overlapped with ESC poised promoters over all poised promoters for each sample.
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PMC11764090
This section explores plants’ cold stress tolerance mechanisms through phytohormones, plant growth regulators, ROS, protein kinases, and Ca.
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PMC9429973
No bias in lineage commitment was reflected upon CD9 blockade, suggesting engraftment of HSPCs is affected in general but not limited to specific subset of hematopoietic precursors.
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PMC9429973
Summary/Conclusion: The study did not establish the influence of polymorphism of the hemostasis system genes (F2: 20210 G>A, F5: 1691 G>A, F7: 10976 G>A, F13: 103 G>T, FGB: -455 G>A, ITGA2: 807 C>T, ITGB3: 1565 T>C, PAI-1: -675 5G>4G) for miscarriage.
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PMC11720808
The diameter of the tumors and the body weight of the mice were monitored every 4 days, and tumor volume was determined using the formula: 1/2(length × width2), this formula was selected based on a previously reported study.26 Lastly, tumors were collected for immunohistochemical staining with antibodies against p-p65 ...
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PMC9429973
Only a minority of HL pts who had additional background diseases or were heavily pretreated, failed to develop an adequate serological response; however, some of them had high Ab titers post-3rd and 4th vaccinations.
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PMC8873393
E Top 3 gene ontology enrichments of the annotated lncRNAs in clusters 2, 4 and 5 with Bonferroni-corrected P values.
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PMC11539788
The next day, 50 µl of Protein A/G magnetic beads was added to each tube and incubated for 2 h with gentle rotation at 4 °C.
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PMC7823217
Sodium alginate with Ca or other divalent cations are commonly used due to fast gelation and minimum adverse effects on cells , , .
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PMC9429973
For 12 studies, trial protocols were available.
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PMC11704135
The radical scavenging activity of the selected pyrimidine derivatives in DMSO was evaluated against a solution of 2,2 diphenyl-1-picrylhydrazyl (DPPH) radical in methanol (37 mg/L), following the method outlined by.
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PMC11291490
Upon employing dusCD40L to stimulate avian PBMCs, we observed its capability to activate the expression of CD40, and promote duPBMC and chPBMC proliferation.
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PMC10854447
In 6a–o, the dimethoxy derivative was the most tolerated for activity.
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PMC9398137
The level of BCL2 protein expression was the major determinant of resistance to S63845: BCL2 serves as a buffer for pro-apoptotic proteins released from MCL1 upon exposure to S63845.
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PMC9429973
The prevalence in Armenia was 2.6:10 during 2010-2012, and 2.7:10 for the next year.
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PMC11320084
TNNT1 drives the migration and invasion of lung cancer (LC) through Wnt/β‐catenin signaling. (
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PMC3888431
The lower part lists all transcripts of the cluster with some information about the functional annotation of the transcript.
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PMC11343332
Conclusion: Overall, this study highlights the promising role of pycnogenol as a proapoptotic and antimigrative agent through the inhibition of anti-apoptotic and VEGF/FGF signaling molecules gene expression, offering new prospects for improving breast cancer treatment.
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PMC11261875
Drug resistance represents a major obstacle to the treatment of GISTs.
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PMC9429973
Approximately 30% of HR-MDS patients are RARA-positive (RARA+) by a blood-based biomarker test (Vigil, 2017).
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