PMCID
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PMC4816271
The retroviral vector used in all constructs was the splicing oncoretroviral vector SFG .
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PMC11747467
We then acquired the expression profile of these DEGs to perform WGCNA based on the six MIBC consensus subtypes ( Figure 1A ).
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PMC9429973
Aims: We aim to describe a single center’s ten-year experience treating PCNSL.
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PMC9429973
Aims: Describe the findings of BM studies performed for thrombopenia at our center.
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PMC10253553
RICTOR is required for mTORC2 activity .
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PMC11740399
2.5 × 10 T24 and 5637 cells were seeded separately per well in a 6-well plate and cultured for 24 h. These cells were treated with the 3025@ML, 3025@ML + OXA, OXA, 3 K, and 3 K + OXA for 48 h. After lysis with RIPA buffer, samples were centrifuged at 10,000× g for 30 min at 4 °C.
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PMC10362265
Three interior regions, one from the IL18 (Fig. 2a, I) and two from the decoy structures (Fig. 2a, II–III), showed high fluctuations.
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PMC9672323
In addition, Leidgens et.al surveyed the metabolic effects of two NSAIDs (ibuprofen and diclofenac) on human glioblastoma cell lines, U-87 MG and A172.
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PMC7553912
Stimulation with OSM did not affect the mRNA expression of MCP-1 in RTECs and renal fibroblasts (Fig. 7b,c).
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PMC5810978
Noroviruses (NoVs) and Sapoviruses (SaVs) are enteric caliciviruses that have been detected in multiple mammalian species, including humans.
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PMC11684269
T cells are pivotal in maintaining immune homeostasis, as they exert multiple functions, including pathogen resistance, immune regulation, immune memory, and tumor surveillance.
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PMC11687167
T11TS immunotherapy and IL-12 protect T cells from apoptosis and promote their survival through the PI3K/Akt signaling pathway, respectively.
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PMC9031474
Karmahapatra et al. also obtained cinnamodial (7) and capsicodendrin (8) from the species Cinnamosma fragrans and tested their cytotoxic and antiproliferative activity against HL-60 and K562 leukemia cells .
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PMC11711663
Higher CVL values indicate better predictive performance of the selected features.
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PMC10125312
PPTT exploits the enhanced optical absorption of noble metal nanoparticles, at their localized surface plasmon resonance (LSPR), to treat malignant cells upon exogenous illumination.
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PMC10850553
The heat map of genes related with the coagulation-fibrinolysis system from the comprehensive gene expression profile data set (GEO accession: GSE161044) is shown.
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PMC8996378
It was demonstrated that compound 78 (5, 10, and 20 μmol/L) alone or in combination with ADR could inhibit protein expression levels of ABCB1, and reversed MDR of gastric neoplasm to anticancer drugs by downregulating the Cox-2/PKC-α/ABCB1/mdr1 signaling pathway in human gastric cancer SGC7901/ADR Cells (Sun and Li, 20...
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PMC11577421
To further validate this finding, we also assessed the uptake of NP_BSA– in enzyme-treated wild-type CHO-K1 cells.
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PMC9919300
Andrographolide treatment reduced approximately two times the expression of the same genes downregulated by curcumin (FABP1, APOC3, PPARGC1A, GK, and SRBF2), while the expression of IL-1β was approximately four times lower.
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PMC11741906
They validated 12 miRNAs using qPCR and identified miR-18a-5p as a tumor suppressor using functional assays in vitro and in vivo.
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PMC9429973
To this aim, we investigated the role of iron on HSCs.
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PMC11323699
The strategy employed to measure circulating TSP-1 levels in OC patients is depicted in the right panel.
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PMC9429973
Very low, low, intermediate and high-risk patients as per IPSET score were 53.9% (21/39), 10.2% (4/39), 20.5% (8/39) and 15.4% (6/39) respectively.
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PMC9118379
Distribution of 320 variant spectra with support by spectral similarity with predicted MS/MS spectra generated by 0–2 intensity prediction tools.
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PMC9429973
Of the 7 responding patients, 1 had frank CD19+CD22+ BM and extramedullary relapse at 3 months and 1 had emergence of MRD level disease at 10.5 months post infusion, in both cases associated with loss of CAR T cells.
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PMC11588008
Immediately use an enzyme-linked immunosorbent assay reader to measure A412, record as a reading value for 0 min, record every 2 min, and record continuously for at least 10 min.
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PMC11144200
Doxorubicin (DOX) represents one of the most potent antineoplastic medications as well as the most widely active compound among similar drugs.
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PMC9812014
In young newborns, it causes disorders like brain microcephaly.
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PMC9587866
Results showed that Sorbifolin had a better IC50 value than 1,7-dihydroxy-3-methylanthracene-9.10-dione, providing further evidence to our screening studies as Sorbifolin established more interactions as compared to the other compound.
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PMC9429973
It concerned all patients with the diagnosis of APL and followed in the hematology department of Hedi Chaker Hospital Sfax, from January 2000 until December 2019.
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PMC11718109
E Co-expression of VMAT2-GFP (green), mCherry-synapsin (magenta), synaptophysin (untagged) and VGAT-FLAG (cyan) in COS7 cells show preference of VGAT-FLAG fluorescence signals in the synaptophysin-synapsin vesicle clusters.
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PMC2777936
The ABC levels were also elevated following knockdown of POU5F1, although to a lesser extent than with RA treatment, between 3 and 7 days post transfection (data not shown).
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PMC8873393
The UV-induced novel lncRNAs and TUCPs were grouped into five classes (Fig. S4B–E, Tables S8 and S9).
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PMC9429973
Results: A total of 57 patient responses were collected during April–November 2021.
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PMC11791264
The cell lysates were centrifuged at 12,000 rpm for 15 min at 4 °C.
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PMC11486946
c Effects of compounds on EGFR oligomerization were evaluated using the KLD (Kullback–Leibler divergence) of the brightness histograms before and after the compound treatment.
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PMC9429973
Overexpression of UCK2 has been observed in several types of solid tumor tissues.
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PMC10317042
Clear cell sarcoma (CCS) is an exceedingly rare subtype of soft-tissue sarcoma (STS) that usually occurs in the lower extremities of adolescents and young adults (1).
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PMC9429973
The ELN genetic risk classification is a tool for treatment planning.
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PMC11188874
A Western blot analysis was performed to determine the level of expression.
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PMC11792740
In depleted T cells, TCR signaling pathways are impaired.
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PMC11674441
Three patients underwent standard adjuvant chemotherapy (anthracycline and taxane) before Neo-P DC vaccine treatment.
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PMC11746942
This genetic manipulation abrogated the anti-hyperplastic effects of TY (Fig. 4G), demonstrating that STAT3 inhibition was critical for the therapeutic effects of S1PR3 antagonism.
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PMC9429973
BCMA mutation and copy number variation were evaluated.
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PMC11725127
The mRNA levels of USP2 and KRAS were detected by q-RT-PCR analysis (B, D). (
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PMC11700165
Cells were serum starved overnight, then serum activated for 10 min at room temperature.
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PMC11746942
We found that S1PR3 expression was markedly elevated in psoriatic lesions.
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PMC9429973
The most represented up-regulated genes related to this pathway were: cyclines (CCNB1, CCNB2, CDK1), centromere genes (CENPE, CENPM, CENPU), kinesin family members (KIF18A, KIF23, KIF2C) and genes involved in cell division (CDC6, CDC7, CDC25A).
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PMC10813895
SATB1 differentially expressed in luminal epithelial cells, and the lineage-specific expression is decreased with age .
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PMC11544122
GST-CXCR4 C-tail was purified separately for each experiment.
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PMC9429973
Thus, it appears to be a promising strategy to ensure high rate of CRFS in very high-risk AML.
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PMC9611901
The samples of natural (bovine) leather used in this study were processed according to the same procedures before tanning and dyeing.
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PMC11098378
Interestingly, some bacterial effectors inhibit innate immune pathways that are also antagonized by viruses, such as the Type I interferon (IFN) response , suggesting that bacterial and viral pathogens may need to evade common eukaryotic defense mechanisms.
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PMC7759933
The spheroids with an initial density of 3000 cells/spheroid and 6000 cells/spheroid were seeded and grown for 12 and 4 days, respectively, depending on the specific endpoint measured.
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PMC11489175
Since RPN11/PSMD14 activity occurs upon ATPase dependent proteasome substrate unfolding, this feature delays its activity until the substrate is committed to proteasome degradation 59.
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PMC2944824
Levels of p53 protein (A) and mRNA (B) were detected by Western blots and Rt-PCR, respectively.
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PMC10291556
In agreement with the literature data, similar effects were also observed for free DCF, however, at concentrations markedly higher (380–770 times).
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PMC11047729
A bioinformatics study on PFOS-associated leukemia showed that VC targets hTERT, amongst other core proteins, which would suggest that it could lower the telomerase activity in vitro and in vivo .
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PMC11314247
SKBR3 and MDA-MB-231 cells are considered ER-negative and have been widely used as in vitro models to test compounds whose therapeutic target is GPER .
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PMC4270159
a. 6 wells per concentration curve × 3 concentration curves = 18 wells + 3 wells = 21 wells per cohort.
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PMC9429973
sBCMA was detected in 100% of patients (n=18).
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PMC10810426
There is still debate regarding the timing and cellular location of HIV’s uncoating and reverse transcription and whether it occurs solely in the cytoplasm, nucleus or both.
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PMC8996378
FAR = 12.1, 23.1 at 20 μM, and FAR = 72.9, 82.2 at 60 μM respectively, on MDR mouse lymphoma cells; FAR = 5.1 and 5.5 at 20 μM on human colon adenocarcinoma cells) (Valente et al., 2012).
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PMC9268620
The microvilli were completely vanished, and blebs started to develop on the surfaces of some cells treated with 10 μg/mL of the compound.
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PMC11597291
Details for the successful synthesis and characterization of GO and CGO are reported in Supplementary Information (SI).
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PMC9429973
The most common reason for not performing a repeat biopsy was having stable radiological findings and other diagnoses deemed unlikely, as depicted in Figure 1.
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PMC11721587
The resultant cell lysates were incubated with Flag M2 (MilliporeSigma) or HA (Pierce) affinity agarose and eluted with corresponding synthetic peptides.
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PMC11512230
The majority of A673 cells after incubation with AltMV SPV were positioned further from the control sample on the chart compared to TMV SP.
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PMC8984067
After the reaction, the product was centrifuged (14,000 rpm), and the mother liquor was removed, with crude solid product washed using 5 mL DMSO thrice and subsequently freeze dried.
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PMC9429973
Since studies to date have had contradictory results, this work aims to assess the risk of developing secondary solid neoplasms (NSS) in patients with CML.
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PMC7709199
This raises the question whether the truncated glycan stub may create artificial protein–protein interfaces.
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PMC10748106
Genipin, on the other hand, shows a divided electrostatic distribution on its substituent in C4 (partially over the sp2 and sp3 oxygens) and a greater electrostatic distribution in the pyran ring, along with the hydroxyl at C1 and C10.
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PMC11285179
Each panel is a representative experiment of at least three independent biological replicates. *, **, ***
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PMC11612794
After blocking with 0.5% BSA in PBS for 5 min, the cells were incubated with primary antibodies diluted in 0.5% BSA in PBS at 37°C for 1 h. The cells were washed three times with 0.5% BSA in PBS, incubated with secondary antibodies diluted in 0.5% BSA in PBS at 37°C for 1 h, and washed three times with 0.5% BSA in PBS.
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PMC11365427
In theory, it is also known that amino acid starvation can provoke ERS, causing cells to activate compensatory mechanisms like UPR.
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PMC9910796
A scheme of the ASFV recombinant, vEP84Ri, in which the expression of the EP84R gene is under the control of the E coli lac operator/repressor system is shown in S3 Fig. Recombinant virus vEP84Ri was generated as described previously .
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PMC11448304
Both upregulated and downregulated modification groups in RA549 were significantly enriched in NHEJ-related processes, and TCA cycle and several amino acids metabolism related processes were also enriched in both groups.
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PMC10870498
ATC is undifferentiated, highly aggressive and mostly chemo-resistant.
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PMC11216402
Gastric cancer (GC) is the fifth most common cancer worldwide and is more prevalent in Asia as well as among Asian Americans in the United States (Lui et al. 2014; Taylor et al. 2015; Ajani et al. 2017).
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PMC11721295
The complete R script, including figure-specific visualization methods, is publicly available on GitHub (https://github.com/Galban-Lab).
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PMC9250505
D pBADS99 levels in EOC cell lines: association between pBADS99 and BRCA-deficiency or platinum-resistance in EOC cells.
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PMC6617630
These results suggest that BET protein regulates the expression of both MYC and BCL6 and the impact of BET inhibition on proliferation (Fig. 2) of DHL and THL cells may be manifested by the coordinated loss of MYC and BCL6.Fig.
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PMC11791203
The pharmacokinetic properties of the compounds identified through GC-MS analysis were evaluated using Lipinski’s rule of five, a set of guidelines that predict the drug-likeness of a compound based on its chemical structure and properties.
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PMC9429973
Image: Summary/Conclusion: The 2-step dose reduction of TKIs before TFR phase is a promising approach for CML pts.
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PMC7090062
GIST-T1 and GIST882 cells were transfected with miR-30a mimic, miR-30a inhibitor or negative controls, then cells were incubated with imatinib (IC50) for 48 h. a, b The efficiency of transfection was validated by qRT-PCR. *
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PMC11576293
However, in this study, we presented the lipophilicity of our novel 2-TPs, which was assessed by both theoretical and experimental methods.
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PMC11536589
The MGST1 mRNA expression levels in tumor group (n = 179) and normal samples (n = 171) of PDAC patients using TCGA databases. (
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PMC11351758
Interestingly, by employing this technique, Ca could not independently induce any significant changes in both TMRM and DCF levels in both the 143B cells and NARP cells, which indicates that for Ca to induce any significant toxicity, ROS presence is a critical factor.
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PMC11739504
White arrows indicate IFT components located in the cilia axoneme.
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PMC11707577
These results demonstrated that, i) the phage‐templated synthesis of TNP preserves the recognition ability of the phage, ii) the M13EGFR(TNP) is efficiently internalized by EGFR‐overexpressing cells, iii) the presence of M13EGFR(TNP) at the cell surface argues in favour of a receptor‐mediated internalization of M13EGFR...
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PMC11476222
As shown in Figure 6C, in this cell line, GM3 was the most abundant sphingolipid with a percentage relative abundance level of 58.1 ± 0.9, followed by CerG3GNAc2, which was 33.6 ± 0.8, and then GD2, GM1, and GM2 with a percentage relative abundance level of 4.90 ± 0.01, 1.60 ± 0.09, and 0.40 ± 0.07, respectively.
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PMC11388384
Cell debris was removed by centrifugation.
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PMC11240052
A diagrammatic sketch of the potential binding sites obtained from the bioinformatics analysis of miR-211-5p in circ_ 0008285 is shown in Table 2.
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PMC11721277
6-(2-(tert-Butoxycarbonyl)hydrazinyl)nicotinic acid (2) (94.6 mg, 0.374 mmol), DIPEA (135 µL, 0.775 mmol), and and HBTU (141.4 mg, 0.373 mmol) were dissolved in DMF (800 µL).
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PMC11496491
After the hybridized chip is scanned by the scanner, the scan map is obtained (Fig. 1A).
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PMC9847912
After a 2-h incubation at 37°C in 5% CO2, 300 μl of complete DMEM was added to each well, and the plates were returned to the incubator for 40 to 44 h. Infected monolayers were treated with fixative, washed twice D-PBS, and stained with 5-bromo-4-chloro-3-indolyl- β-D-galactopyranoside (X-gal) as previously described ;...
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PMC4270159
Lapatinib blocks NRG1-induced resistance to PLX4032, restoring sensitivity. •
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PMC9684669
GAPDH was used as a loading control. (
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PMC2944824
Several studies have suggested that this maturational change in the mechanism of cell death is regulated, in part, by thyroid hormone.
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PMC10047551
The expression of PD-L1 by the NSCLC cells determines treatment effectiveness, but the relationship between PD-L1 DNA methylation and expression has not been clearly described.
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