PMCID
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PMC9429973
Factors influencing OS in univariate analyses were Sorror comorbidity score ≥3 at 1 Allo-SCT (1 year OS 17% vs 32%, p=0.036), time to relapse after Allo-SCT < 6months (1 year OS 16% vs 35%, p=0.020), and intensive salvage treatment (1 year OS 33% vs 24%, p=0.019).
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PMC9847912
GSK232 was highly active against HIV-1 isolates from group M subtypes A, B, C, D, F, and group O, with IC50 values ranging from 0.25–0.92 nM in spreading (multi-cycle) assays and 1.5–2.8 nM in a single cycle of infection.
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PMC9672323
The graphs show the mean ± SD.
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PMC11578343
The signals are aligned in time; resistive pulses cascade (starting from the sizing pore to zone 1, zone 2, etc.)
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PMC11726183
This indicates the possibility of the formation of tautomer in some of the synthesized compounds.
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PMC11471176
Amounts ≤10 mg of Carnosine were dissolved in water, dimethyl sulfoxide (DMSO, 11 %) (CAS 67-68-5, Santa Cruz Biotechnology, Inc., Dallas, Texas, USA), appropriate media as detailed in sections below, and a mixture of DMSO - media.
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PMC9429973
Lonca-R treatment consists of R intravenously [IV] 375 mg/m on day 1 of cycles 1-4 (subcutaneously allowed starting at C2), Lonca 150 µg/kg IV on day 2 of cycle 1 and day 1 of cycle 2, and Lonca 75 µg/kg IV on day 1 of cycles 3 and 4.
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PMC10329074
After the cell suspension was collected, the cells were washed with PBS, subsequently pre-cooled 75% ethanol was added in a vortex mixer, and the cells were fixed at 4 °C for 2 h. Finally, cells were stained with propidium iodide for 30 min in the dark.
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PMC10587429
For comparisons between two independent groups with approximately normally distributed variables, unpaired, parametric two group t-tests were used.
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PMC10656496
Under coculture conditions, when 143B was given an advantage in numbers (90% 143B vs. 10% SAOS2, the 90/10 coculture), 143B was able to outgrow and therefore outcompete SAOS2, rendering it undetectable even when deprived of nutrients (Fig. 1).
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PMC10944868
Finally, the Lipinski’s Rule of Five, a widely used rule in drug discovery to assess the drug-like properties of a compound, was performed to study our compounds.
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PMC9429973
Patients with IE significantly differed from the group with known causes in haematocrit levels (p=0.0219) with lower levels in IE patients.
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PMC6133382
Glycosylation heterogeneity is known to result from a variety of variables, including cell type, protein expression levels, cell culture conditions, monosaccharide donor availability, and protein structure [30,33–36].
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PMC11741577
Transwell assays of HaCaT migration and invasion after incubation for 24 and 48 h. Upper chambers contained HMC-1 CM (n = 3). (
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PMC11119602
The primer sequences used for the RT-PCR of GAPDH and TG2 are shown in Table 1.
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PMC9429973
The terminal elimination half-life was approximately 32 days.
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PMC9429973
We observed a higher proportion of patients with AML and a lower proportion of patients with CCUS in patients with a RUNX1 variant compared to patients without a RUNX1 variant, and this tendency was enhanced if the RUNX1 variants were present at VAF > 40%.
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PMC9065483
The above experiment was then repeated using vanadate-inhibitory concentration ranges of gefitinib and UO126, and infected cell supernatants were titered for viral output assessment by standard plaque assay (Figures 2D and S7).
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PMC11693599
A positive correlation was found between plasma CD109 levels and FIGO staging in ovarian cancer patients (data not shown).
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PMC9429973
The risk of total infection (CTCAE any grade) was significantly lower (pooled RR 0.92; 95%CI 0.85-0.99; P=0.03) in patients on acalabrutinib containing regimens; however, there was no difference between CTCAE grade 3+ infections between the two groups (Figure).
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PMC9822769
Furthermore, in monochalcoplatin, the presence of the two equatorial chlorido ligands appears to be essential for its striking uptake since the oxaliplatin-based Pt(IV) analogue appears to be deprived of any transport mechanism .
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PMC9436459
Activity (IC50 in μM); Ki value; Inhibition typePositive control (IC50 in μM)Mulberrofuran J (1)0.60 3.31 (OA)Albasin A (3)1.59 3.31 (OA)Chalcomoracin (5)1.9 3.0 (OA)Mulberrofuran C (6)4.9 4.5 (RK)0.72 3.31 (OA)Albasin B (8)5.12 3.31 (OA)2.80; Ki = 1.00 μM; Noncompetitive 9.47 (UA)Morusalisin C (10)1.58 1.70 (CCF)Morbi...
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PMC5415764
At 1, 2, and 4 h post-incubation at 37 °C, the cells were washed twice with cold PBS to remove drugs from the cell surface, collected and resuspended in 0.2 mL of RIPA lysis buffer (Beyotime, China).
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PMC11271278
Several analysis approaches have been developed to identify enhancer hijacking.
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PMC11706776
Knowing that ion channels are involved in tumorigenesis, this work aimed to study the involvement of the KCNN1 gene, which encodes the SK1 potassium channel, in ES development.
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PMC11629143
Although it is a powerful expression system, we found suboptimal histone acetylation at the CMV promoter in CHO cells.
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PMC11106977
Yang et al. reported that nanocarriers allow selective delivery of OGT inhibitors or other compounds to target cells, thereby improving the efficiency of O-GlcNAcylation targeting .
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PMC9966931
The use of hybrids (4) and (6) at 0.5% completely prevented the growth of fungi.
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PMC11707832
Reduced α-mannosidase activity affects not only the nervous and immune systems but also involves other organs, leading to liver or spleen enlargement, lung issues and kidney complications .
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PMC10440586
Univariate Cox regression analysis of eight cohorts confirmed that CD99 upregulation is a risk factor for patient survival, with six of the eight datasets showing a significant P-value and all hazard ratios over 1 (Fig. 4E).
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PMC11001582
NS denotes P > 0.05.
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PMC9429973
No difference was found in relapse rate or DFS.
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PMC8389560
We used connexin mimetic peptide (Gap26) to inhibit Cx43.
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PMC6379402
Early integration approaches first combine all datasets into a single dataset from which the model is built.
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PMC11786767
The sample sizes “N” and “n” indicate the number of examined cells and chromosomes, respectively.
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PMC9895440
e Average expression of selected marker genes (color).
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PMC11471176
Nevertheless, confirming that carnosine and LMS1007 both have breast cancer and COVID-19 activities in the same ACE2 inhibition range adds more robustness to the findings of this study.
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PMC11806818
Yang et al. also proposed a portable photothermal cell sensor for high-sensitivity detection of MCF-7 CTCs.
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PMC11697703
Also, layer II-III neurons expressing both miR-193b-365 in S1 showed decreased MAPK8 compared to non-electroporated layer II-III neurons in either Contra-S1 or Ipsi-M areas (Figure 2J).
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PMC11599565
This finding suggests that some resident cells in the normal ovary may possess neuronal or neuroepithelial characteristics, which persist in ovarian carcinoma tissues.
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PMC11514418
Comparison of short-term efficacy, n (%) APS: Astragalus polysaccharide; GS: Gemcitabine plus tegafur gimeracil oteracil potassium capsule (S-1); ORR: Overall response rate; DCR: Disease control rate.
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PMC10553757
H. Rostom: None.
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PMC11271278
Source data are provided as a Source Data file.
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PMC8658661
As a result, metabolic activity, including cytochromes, is higher in 3D spheroidal HepG2 models than in 2D cells , bringing it closer to normal hepatocytes.
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PMC11126803
In this study, we further confirmed that ERβ can promote the invasion and metastasis of ccRCC, whether in vivo or in vitro experiments.
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PMC9429973
Summary/Conclusion: GVHD of any type or grade is not associated with reduced relapse rate after haplo-identical SCT with PTCy in ALL.
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PMC8427838
Image magnification, 50 µM. White arrow points to filopodia-like protrusions.
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PMC11594074
Two compounds were from the ZINC15 subset, and two were from the FDA-approved drug library.
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PMC11528603
All these results strongly suggested the anticancer property of SA-EA.
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PMC11172027
NASeLM showed a caspase-3 activity of 29.1 ± 3.1% (n = 3) already after 24 h with a concentration of 250 µg/mL and was significantly higher than the 24 h baseline control (14.3 ± 2.4%; n = 3; p < 0.01) and the 24 h 250 µg/mL NALM sample (14.7 ± 2.5%; n = 3; p < 0.01).
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PMC10831439
After transduction with a retroviral SFG.CD19-CAR construct (figure 1A), iNKT cells were expanded during two stimulation rounds (weekly) with feeder cells, IL-2 and α-GalCer.
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PMC9351137
B Histograms showing the quantitative analysis of mislocalizations of cytokinesis regulators at the midbody in mocks and BAF53a or Tip60 depleted cells.
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PMC8873393
1The ability of human fibroblasts MRC5_VA cells to repair UV-induced DNA lesions.
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PMC9429973
During neutropenia after first cycle, Filgrastim (5 μgr/kg/day for 3 days) was given if neutrophils count was <1500 x 10^9 cells/L. Results: Median number of filgrastim administrations was 4.6 (r. 3-6); nadir neutropenia was registered after a median of 10.4 days (r. 7-14); median of nadir neutrophil count was 1.13 x 1...
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PMC9429973
Overall, 38% of patients achieved a complete response with a decrease in response in later lines (2L: 70%, 3L: 58%, 4L: 29%, 5L+: 33%).
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PMC11781181
Quality control (QC) sample was obtained by pooling all prepared samples and served to equilibrate chromatographic system and correct variations during lipidomics instrumental analysis.
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PMC11658074
f CD151 expression in CD151( +) AT-EVs and CD151(-) AT-EVs.
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PMC11472639
In the CD3CD56 NK-cell population: CD56 and CD56 NK-cells were identified in a FSC-A × CD56 plot; NK-cells capable of, and susceptible daratumumab-mediated ADCC were identified in a CD38 × CD16 plot; and CD57 and CD57 NK-cells were identified using a histogram.
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PMC9219615
Samples were stained with a nuclear isolation medium containing the fluorophore DAPI (NIM-DAPI) post-incubation.
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PMC11543853
A selected empty particle is indicated by a red arrowhead.
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PMC7305184
Electroporation pulses alone do not cause/influence cells to develop adaptive resistance to cell permeabilization caused by electroporation.
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PMC10507284
p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, not significant.
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PMC11754094
However, the frequency of large deletions due to AYBE at the target sites without TC motif was substantially higher than that of ABE.
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PMC11676169
T-ALL cells were seeded on Shi-fix coverslips (SB-Shifix50PLUS; Shikhar Biotech, Nepal) according to the manufacturer’s instructions.
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PMC11730311
Inhibition of hαCGRP-stimulated cAMP generation in CHO-K1 expressing the hCGRP-R. Concentration–response curves were generated in the absence or presence of (a) hαCGRP8-37 or (b) hαCGRP8-37N25K-C20DA-γGlu. (
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PMC10729469
These results fulfil an unmet need as recent FDA-approved antibody therapies for AD have met with mixed success .
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PMC11772449
However, with the prolongation of the administration time, in the later stage of the administration stage, it can be seen that the combination group had significant cumulative toxicity, and the weight loss of mice was more obvious (Fig. 7 D), suggesting further in-depth studies are warranted before clinical application...
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PMC9429973
Furthemore, we found that five out of ten drugs enhance antibody-dependent cytotoxicity of rituximab, in functional assay.
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PMC11745600
Blood glucose levels of engrafted Ins1 NOG mice started to decrease from 1 wk, reaching below 200 mg/dL by 6–10 wk posttransplantation (Figure 6A).
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PMC11611077
Bands at around 100 kDa were observed from Lanes 2 and 3 (samples; CHO cells transfected with His-tagged prestin (EF1α3C8)) while it was not detected from Lane 1 (negative control; CHO cells transfected with FLAG-tagged prestin).
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PMC11621565
Real-time cell monitoring of SH-SY5Y cells in the proliferative state using growth medium (supplemented with 10% fetal bovine serum) vs. SH-SY5Y cells that follow the dopaminergic differentiation protocol.
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PMC11670954
Relative mRNA expression levels were normalized and are expressed as the fold change relative to the expression level in the control group.
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PMC11578343
The working principle behind single‐cell viscoelastic measurements using an electronics‐based node‐pore‐sensor was previously described by J. Kim, et al. Fundamentally, the electric resistance across a microfluidic channel was proportional to a transiting cell's diameter—a larger cell will partially block more current ...
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PMC11695623
Different immune cell infiltrations in DCUN1D5 high and low expression groups in the TCGA-LUAD dataset. (
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PMC11748618
In the context of HIV disease, where the burden of illness is predominately in African populations and approximately 25% of variance of HIV spVL is attributable to host genetics, there is an opportunity to identify new HIV host dependency and restriction factors in underrepresented populations.
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PMC9429973
Pirtobrutinib is a highly selective, non-covalent (reversible) BTKi that inhibits both wild type (WT) and C481-mutated BTK with equal low nM potency.
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PMC9429973
No serious adverse reaction was reported among patients with further treatment.
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PMC11533140
Thus, we selected it as a candidate for further research and have filed a patent covering these structures (Li et al., 2019).
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PMC10994876
A substantial increase in the S phase was observed in OE-YAP5SA cells compared to Mock and NT.
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PMC11695546
BChE is primarily expressed in white matter, glia, and different populations of neurons.
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PMC11790971
In addition, the ridgeline plots in Fig. 4g–i show the distribution of features in high-to-low feature ranking groups for each feature category.
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PMC9429973
One of the most important pathogenetic mechanisms of the development of PMBCLs is their genetic heterogenety.
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PMC11295144
Cells were blocked in 1% bovine serum albumin (BSA, BP9704-100, Fisher Scientific) in HBSS for 30 min at room temperature and subsequently incubated with anti-HA tag primary antibody (1,10,000 in blocking solution, ab184643, Abcam) for 2 h at room temperature or overnight at 4°C.
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PMC5673060
This study provides a useful resource for future studies investigating the determinants of pazopanib and dasatinib resistance; and identifies a new therapeutic strategy of inhibiting HSP90 function for further evaluation as a means of overcoming pazopanib and dasatinib resistance and tumour recurrence in multiple cance...
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PMC11694625
Results of the apoptosis study showed that compounds 3b and 3c increased the number of late apoptotic and early apoptotic cells.
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PMC11705901
For rs1042778, there are no SNPs with D’ over 0.12, suggesting it is in low linkage with other OXTR SNPs.
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PMC2614415
The sub G0/G1 population represents apoptotic cells.
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PMC10452486
Examining the immunoprecipitates of IFN-γ-treated and untreated cells by SDS-PAGE, they found a greater increase in the synthesis of HLA B2m-free HCs (Face-2) in IFN-γ-treated cells than in untreated cells; a similar greater increase in treated cells was not found for intact HLA (Face-1).
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PMC11585565
The full length CNNM4 cDNA was amplified using Q5 High-Fidelity DNA polymerase (New England BioLabs) and the primers are shown in Supplementary Table S7.
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PMC11699178
Initially, we identified 9 genes directly interacting with HIF-1α among the DEGs (Fig. 3A).
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PMC11536589
Heatmap of several critical gene expressions of the Wnt/β-catenin signaling pathway in AsPC1 cells and AsPC1-MR cells. (
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PMC9429973
Containers with cell suspension were stored in liquid nitrogen.
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PMC11751675
For instance, centaurepensin contains two chlorinated groups, while chlorojanerin has one.
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PMC11528603
Moreover, p21 is also involved in initiating apoptosis.
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PMC11291697
A Array-CGH (180 K and 1 M platforms) profile showing the de novo 2q22.3 microdeletion removing exons 5 and 6 of GTDC1.
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PMC10900886
The detection of VP1 and the absence of new infectious virus particles in PBMC B cells can be explained by several potential mechanisms: (1) B cells are present in low number in the circulation (~4%–14%) (32).
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PMC11394730
During the same investigation, growth factor-reduced matrigel and A549 cancer cells were administered subcutaneously into BALB/c nu/nu nude mice.
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PMC11469524
Since inhibitor dosages were always the same but the response to the inhibitors varied from cell line to cell line, the actual difference is thought to happen on the internal wiring not on the inhibitor perturbation strength coefficient.
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PMC10506676
The final results were calculated based on the average of corresponding experiments for each group.
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PMC11365427
We discovered that BCH effectively suppressed the survival of MM cells.
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