PMCID
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PMC10933092
In contrast to mA, the distribution of mA in mRNAs is unique in its proximity to the start codon, a pattern distinct from the 3′UTR enrichment of mA (Meyer et al., 2012).
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PMC9429973
Follow-up data were censored at February 1 2022.
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PMC10958426
DEGreg, showing low CNV-expression correlation and mainly in low CNV variation regions and regions without CNVs, enriched in cytokine signaling genes.
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PMC9429973
With a median follow-up of 80 months (17-161), 5-year Overall survival and event-free survival were respectively 67% and 65%.
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PMC11543885
Filter Data: Focus on proximity proteomes with SAINT probability >0.8 and fold change (FC-A) ≥1 in either unstimulated or stimulated samples.b.
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PMC11738017
The median progression-free survival (PFS) and overall survival in the 27 patients were 10 and 12 months, respectively (Figure 1A-C).
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PMC10582049
Illustrations are created with BioRender.com.) (
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PMC11711063
The suppression of the pro‐tumorigenic, ZG16B‐promoted matrisome and motility, along with an anti‐Warburg shift in glucose uptake in vivo may represent new hallmarks of the concerted tumor suppressive action by Bex + Carv.
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PMC11721277
The resulting mixture was stirred for 20 min at 25 °C, after which taurine (33 mg, 0.264 mmol) was added.
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PMC10667689
Significance of the differences after 24 h, 48 h, and 72 h compared to the control group and PMA group in HL-60 cells.
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PMC11721587
Data are means ± SD. *
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PMC11461874
Statistical analysis of the data was performed using a two-tailed Student’s t test (c) or one-way ANOVA followed by Dunnett’s test (d).
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PMC9479186
The Complex I consists of 45 subunits, some of which are involved in electron transport, some form the transmembrane structure, and some maintain function and stability (32, 33).
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PMC9429973
Thrombocytopenia with platelets < 100x10/l occurred in 10 of 11 patients with mean platelet of 14 x10/l.
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PMC9895440
T2 cells (ACC598, RRID:CVCL_2211) and Jurkat (ACC282, RRID:CVCL_0065) were purchased from DSMZ, Leibnitz Institute.
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PMC9927933
Supernatants were saved for direct IB after centrifugation.
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PMC11752767
Thus, adoptive cell therapy (ACT), employing immune cells with specific tumor-recognizing receptors, has emerged as a promising therapeutic approach to display on-tumor toxicity.
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PMC8992508
The compound did not behave as efflux transporter substrate and showed good in vitro ADME properties.
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PMC11727062
6-Shogaol, an alkylphenolic compound derived from Zingiberis rhizoma, exhibits a broad spectrum of pharmacological actions.
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PMC9429973
Lymphoblasts for all pts expressed CD22.
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PMC11798926
Interestingly, in native AMPAR complexes SynDIG4 associates at the interface of GluA1 and another AMPAR auxiliary factor cornichon-2 (Yu et al., 2021).
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PMC6892818
Epithelial cells were either (a) cultured in 24-well plates at 5 × 10 cells/well and treated with 10 µg/ml of cross-linked G28-5 mAb or (b) co-cultured at 5 × 10 cells/well with 6 × 10 cells/well growth-arrested 3T3CD40L and 3T3Neo in 24-well plates.
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PMC11593713
Supernatants were removed, and the obtained cells were suspended in PBS (without calcium and magnesium ions) and stored at −80 °C for further examination.
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PMC7171147
Luminescence was recorded over time using a Glomax Explorer Microplate Reader (Promega) and was normalized to total protein content.
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PMC10812665
To obtain rich data sets, we analyzed transcriptome changes, checked for alterations in the biochemical functions and checked metabolic perturbations of the neurons (Figure 1E).
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PMC9646302
GAPDH was used as an internal control.
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PMC2614416
Similarly, for the set of bioinformatics calculations whose BLAST searches are carried out using BLASTP 2.2.15 [Oct-15-2006], oxidoreductases are down-regulated in HepG2 cells by 56%.
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PMC9454178
A Dose–response curves of GIST-T1 and GIST-882 cells after treatment with THZ1 for 72 h and 144 h, respectively.
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PMC11045125
The same extracts used in Fig 5A were used for this blot and the Myc, LMP1, and actin blots from Fig 5A are reproduced here to show levels of expression in each condition.
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PMC8097906
HA-OH and A204 cell migration was impaired to the same extent as is evident for Rh30 (Fig. 6g).
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PMC11301242
Data were log2 converted. (
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PMC7887261
Genetic ancestry analysis shows 90% TCGA-LCs were inferred as genetic European ancestry.
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PMC10317042
Data in A, D, and E are means ± SDs. *,
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PMC10582049
The experimental protocols were approved by the Vanderbilt University Medical Center Institutional Review Board (IRB protocol #000616).
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PMC11589157
For transcriptome sequencing, lymphoblastoid cells and liver tissues from male N. leucogenys, frozen in liquid nitrogen, were obtained from the tissue archive of Kunming Cell Bank.
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PMC9587298
Fig. 3, shows the negative ion MS spectra of the main flavonol standards well know from elderberry (Fabre, Rustan, de Hoffmann, & Quetin-Leclercq, 2001), namely quercetin-3-glucoside ([M – H] at m/z = 463), quercetin-3-rutinoside ([M – H] at m/z = 609) and quercetin ([M – H] at m/z = 301).
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PMC11536589
The BxPC-3 cells, with wild type KRAS, and the MIA PaCa-2 cells, which harbors KRAS mutation, exhibited comparative resistance to MRTX1133.
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PMC9429973
This was most pronounced in B-CLL patients who had an age and sex adjusted risk ratio (RR) of 12.25 (95% CI: 6.42-23.38, p< 0.001) of humoral non-response compared to healthy controls.
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PMC11585254
For example, dileucine motifs in chemokine receptors CXCR2 and CXCR4 are required for their internalization following agonist stimulation (Fan et al., 2001; Orsini et al., 1999).
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PMC7185206
In brief, paired mouse IgG2 monoclonal antibody clones H1A and B11 against extracellular human PD-L1 were utilized in a capture-detection plate assay using biotinylation and HRP-streptavidin detection.
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PMC11087301
P123 as a new peptide agonist acting like BMP signaling can suppress transforming growth factor-β (TGF-β) and facilitated EMT in primary malignant cells.
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PMC11731161
p<0.05, **p<0.01,*** p<0.001, and **** p<0.0001 USP7 deubiquitinates and stabilizes XPO1 protein in MM cells.
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PMC11506670
To achieve 80–90% transfection, HCT116 cells were seeded into 12-well plates at 150,000 cells/well, while NIH3T3 cells were seeded in a separate set of 12-well plates at 100,000 cells/well.
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PMC11244823
All fragments generate significantly (p-value <0.05) lower RLU values for the negative control than the positive control.
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PMC11011837
For polar metabolite analyses, from each cell line, 5 experimental replicates were prepared in 5 different T-75 flasks.
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PMC10114490
For instance, they are not suitable in drug screening for hepatic inborn diseases such as glycogen storage or alpha-1 antitrypsin deficiencies.
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PMC11486946
The difference in the brightness between these regions was calculated for each time point and normalized to the value at time 0 (Fig. 4e).
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PMC11106977
Both isomers contain the same N-terminal catalytic structural domain (Catalytic) (orange) and stalk structural domain (Stalk) (green), but the C-terminal HAT-like structural domain (pHAT) (blue) is absent in sOGA O‑GlcNAcase (OGA) is the only enzyme capable of hydrolyzing O-GlcNAcylation modifications from various glyc...
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PMC11786767
D: Confocal images of PicoGreen signals in entire Post-Ex cells and enlarged chromosome segments.
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PMC7452526
Statistical significance was assessed by a one way ANOVA: ∗∗p < 0.05 was considered significant; ∗∗∗p < 0.001 very highly significant compared with the control.
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PMC6364197
The prognosis of many hematological malignancies has greatly improved because of the new drugs (30).
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PMC9429973
These finding open the field for well-designed RCT to develop novel treatment options for CIPN targeting CIED.
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PMC11779605
Higher expression of FGF7 in tumor compared to ascites cells was validated at the protein level in two replicates of the orthotopic model and in an independent immunocompetent mouse model using the syngeneic murine ID8 Trp53 luc cell line.
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PMC11321678
These results demonstrate that inhibiting subgroup‐specific NTR genes may represent a potential therapeutic target for the treatment of MB.
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PMC7961460
Moreover, the treatment of MCF-7 cells with NHDF CM did not alter neither HAS2 nor HAS3 expression (Figure 6D,E), suggesting that the HA measured in Figure 6A is predominantly produced by NHDF.
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PMC5916734
Production of the cell lines secreting biologically active FIX FIX secreted by the 3B12 clonal cell line was almost completely inactive.
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PMC11413393
For inducible cell line studies, dox treatment was initiated once tumors reached between 100-200 mm and was delivered into the drinking water at 1 mg/ml.
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PMC3915447
C. Western blot analysis for the determination of caspase-3, bax, bcl-2 and survivin expression Our results have shown that the caspase-3, bax, bcl-2 and survivin proteins, in the CCRF CEM cells, treated with 50 µM FA had no significant changes as compared to control group (p<0.05).
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PMC11045125
This result suggests that high level Myb expression in established human BLs contributes to loss of LMP1 expression by increasing DNA methylation of the LMP1 promoters via DNMT3B and UHRF1 activation.
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PMC11335267
Although Alx3 expression in adult tissues appears to be relatively restricted to discrete locations, the use of a constitutive knock out model represents a limitation, and the confirmation of a specific role for Alx3 in the regulation of Agrp and Pomc neurons in the arcuate nucleus awaits neuron-specific inactivation o...
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PMC9429973
Due to dizziness, one patient had a dose interruption and after 5 months of therapy discontinued treatment from new onset of atrial fibrillation and pulmonary hypertension (unrelated to SEL and RUX).
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PMC11730305
It can induce apoptosis and death of cancer cells by activation of apoptosis mediators, including caspases, the ratio of BAX/Bcl-2, and p53.
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PMC11792888
Other natural products have been shown to heighten the effectiveness of etoposide .
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PMC9429973
These findings warrant to be further investigated in larger series.
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PMC11218145
As a matter of fact, it has been shown that GGOH is an inhibitor of the 3-hydroxy-3-methylglutaryl-CoA reductase for the upstream production of mevalonate acid .
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PMC11695623
qRT-PCR to detect the knockdown efficiency of DCUN1D5 (B) Effect of down-regulation of DCUN1D5 on cell proliferation ability. (
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PMC9019892
GIST‐T1, GIST‐882, GIST‐5R and GIST‐48B cells were treated with DMSO, serial dilutions of nintedanib (0.03–1 μm), 1 μm imatinib or 1 μm sunitinib for 24 h and 72 h. The cells were then collected and analysed by western blotting using PARP, β‐actin and caspase3 antibody as described previously .
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PMC11194678
All statistic data are presented as mean ± SD; *P < 0.05, **P < 0.01 using one-way ANOVA with Tukey’s multiple comparison test.
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PMC11711127
Due to membrane damage and release of damage associated molecular patterns, ferroptosis is immunogenic.
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PMC11715644
Naturally, the traversal of cell collective through confining environments involves crowding due to narrowing spaces, which seems tenuous given the conventional inverse relationship between cell density and migration.
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PMC8711270
This was achieved by generating Sf9 cell lines stably expressing the AAV rep and cap genes (Mietzsch et al., 2014, 2015, 2017).
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PMC11718031
In comparison, the cell culture using CD-CHO produced only 0.25 g/L mAb.
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PMC11597167
BACE1-mediated cleavage of APP is considered the rate-limiting step in the production of Aβ peptides.
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PMC11730311
Data point represent mean and range of n = 2, except for C20DA-γGlu protraction at position 17 where n = 1.
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PMC11139449
The clonal selection of EGFP expressing CHO- K1 cells.
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PMC11461874
The exon 4 is skipped in TRAF7 KO cells by using CRISPR-Cas9 genome editing.
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PMC11509224
They share a trans-fused [4.4.0]-bicyclodecane system with a hemi-acetal functional group bridging C-8 and C-10.
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PMC9429973
Median age was 76 (range 47–87) years, 10 (67%) patients had an IPI score of 3–5, 7 (47%) had ≥2 prior therapy lines, and 8 (53%) were refractory to last treatment.
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PMC11190538
Crystal violet was used in this study to produce more objective findings.
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PMC11774251
As a widespread cutaneous disorder, psoriasis manifests as persistent inflammatory lesions characterized by abnormal keratinocyte proliferation and the infiltration of various immune cells.4,17 Psoriasis treatment focuses on disease management, slowing progression, and reducing symptoms and recurrence.6,18 Recent advan...
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PMC11269933
The PK activity assay (C–E) (n = 3; C), PEP content ratio (n = 3; D) and PA content ratio (n = 3; E) of C. gigas and C. angulata reared at northern (Qingdao, 35°44′ N) and southern (Xiamen, 24°33′ N) sampling sites.
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PMC11155445
The highest activity of compound (76b) was due to the presence of a stable C–Cl bond, which interacts with biomolecules and the presence of σ-hole interactions in complexes with biomolecules (Fig. 20).
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PMC9429973
VITT has raised concerns regarding the possibilities of increased thrombotic risk and thrombocytopenia after anti-COVID-19 vaccines.
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PMC11352666
Downstream signaling was also inhibited in cells treated with MRX-2843.
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PMC11026382
In the laboratory, strains with reduced TYMS activity could provide a less stringent context for testing designed sequences or evolving new DHFR function.
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PMC10713411
Finally, we illustrated that TRIB3 might be a protumor factor responsible for the elevated proliferation and invasion capacities of renal cell carcinoma (RCC) cells.
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PMC11506827
Thus, combining GEF with TRAIL is a promising strategy for TRAIL-resistant cancers .
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PMC9429973
Sequencing was performed using a MiSeq (Illumina) with the 30-gene panel Myeloid Solution (SOPHiA Genetics).
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PMC9429973
Aims: The aim of the study is to investigate the factors associated with the long-term survival of R/R B-ALL patients after CAR-T therapy.
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PMC7351993
Good agreement between the theoretical estimate and experimental results firmly demonstrated the real-time sorting capability with the Raman image contrast (see “evaluation of the sort purity and yield” in the Methods section for details).
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PMC9878278
Further, TLR3 activation was found to be associated with the depletion of neural progenitor cells in ZIKV-infected human cerebral organoids (Dang et al., 2016).
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PMC11768281
This figure illustrates the pre-activation phase of T cell responses during Plasmodium infection.
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PMC11576293
2H, SCH2CH = CH2), 3.89 (s, 3H, OCH3), 1.55 (d, 6H, SCH(CH3)2, J = 6.6 Hz); C NMR (75 MHz, CDCl3) δ (ppm) = 171.7, 164.4, 163.2, 162.2, 137, 135.4, 134, 129.1, 128.8, 128.5, 117.5, 114.3, 106.8, 55.4, 34; LRMS (ESI+) m/z (%) 371 (M + H+2, 38), 369 (M + H, 100).
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PMC11545868
Prunetin, a flavonoid, is an O-methylated isoflavone with a structure based on a 2-phenylchromene-4-skeleton.
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PMC11772449
Fig. 7In vivo experiments confirmed the synergistic anti-tumor effect of PFD and Sora.
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PMC11786704
In vivo experiments showed that the PC capsule-treated group exhibited significantly accelerated wound healing and tissue regeneration, along with reduced levels of inflammatory factors compared to the control and PC solution groups.
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PMC11592837
We also noticed a decline in foci number for both ETP and DOX treated cells after more than 1 h of incubation.
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PMC11686380
The association between GAP43 and neuropsychiatric disorders has also been reported to date.
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PMC11594588
Increased calcium levels have been found in mitochondria, which also correlate with ROS imbalance in mitochondria and activation of the MPT pore.
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PMC7504302
The authors further evaluated the role of CDK1 (cyclin dependent kinase 1), NDC80 (kinetochore complex component), and PLK1 (polo like kinase 1) proteins in cell survival since they could be targeted by small-molecule inhibitors and have not been linked to MPM before.
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