PMCID
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PMC9429973
Twenty-one patients (55.3%) were stage 3b.
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PMC11680578
Hence, synergistic treatment of PTX with another molecule that can target similar or other mechanisms involved in the development of ovarian cancer is required.
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PMC11791264
Tubulin (BS1699) and GAPDH (AP0063) antibodies were obtained from Bioworld Technology.
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PMC9621239
Clones of HBV X genes harbouring mutation L123S, double mutation S101P/L123S and triple mutation T81P/S101P/L123S are displayed in the Sankey diagram. (
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PMC9429973
Results: Median follow up was 16 months (range 5-117 months).
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PMC11489351
However, MDM2, a negative regulator of NUMB, is differentially regulated with high expression at high pHi and low expression at low pHi.
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PMC11394730
By lowering tumor-promoting macrophages and inhibiting EMT and CSC development in the primary tumors, short-term emodin treatment prior to surgery prevented breast cancer post-surgery metastatic recurrence in the lungs.
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PMC10577955
Several reports have shown that treatment with betulinic acid has a prominent effect on multiple human cancer cell lines one of them is lung carcinoma .
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PMC10093184
D-17 cells fully closed the scratch within 60 h. Detailed quantification of the cell-free area in all cell lines over the tested 60 h period is summarized in Figure 4A.
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PMC11332076
The DNA sequences were obtained from GenBank (NM_010568.3 and NM_000875.2).
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PMC10170482
Fluorescence digital images were acquired via either a 20× objective LUCPLFLN20XPH NA 0.45 air lens (Olympus, Tokyo, Japan) or 100× silicone immersion objective UPLSAPO100XS NA 1.35 WD 0.2 silicone lens (Olympus, Tokyo, Japan).
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PMC11098378
C-terminal IpaH4-Flag was constructed via restriction cloning into pCDNA3.
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PMC11190238
In light of approaches that focuses on local chromatin conformation of a handful of genes, low throughput chromatin conformation capture methods could prove to be useful such as 3C, where the interaction frequency between two or more interacting regions could be quantified.
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PMC10197932
Further analysis will be conducted, such as co-culturing with AML cells after US treatments, to determine NK or CD8+ lymphocyte cytotoxicity.
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PMC8358006
However, loss-of-function mutations leading to a decrease in PRC2 activity and affecting the H3K27 substrate of PRC2 have been found in several types of cancer, resulting in a decrease in the global level of H3K27me3.
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PMC7519871
Binding site for each protein target was detected using DOG Site Scorer.
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PMC9429973
Among the treatment-emergent adverse events (TEAEs) more commonly associated with luspatercept were bone pain, arthralgia, dizziness, hypertension, and hyperuricemia.
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PMC9429973
Risk of any infection was increased even in C282Y homozygotes with normal levels of plasma iron, transferrin saturation or ferritin.
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PMC11585565
The statistical analyses were performed using GraphPad Prism version 9.0.
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PMC10734950
Following the incubation, 300 μl of PBS was added to each flow tube and read immediately via a flow cytometer.
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PMC9429973
Light chain mass spectra were obtained using EXENT software to yield immunoglobulin isotype, mass-to-charge ratio (m/z) and quantity (g/L).
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PMC11488203
The EV/exosome isolation methods from untreated MSC (control) or taxol-treated MSC (taxol) by differential ultracentrifugation (UZ) and/or SEC were compared by Western blot analysis for expression of tetraspanins.
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PMC7685376
First, we performed GO enrichment analysis on BP, MF, and CC for the 160 differentially expressed proteins.
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PMC11437637
The Shapiro-Wilk test was used to determine whether the data were normal distribution or not.
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PMC11012012
They found that SLAMF1/CD150 expression was positive in activated B cell DLBCL (ABC-DLBCL) (three out of three cases) but negative in germinal center DLBCL (GC-DLBCL) (nine out of nine).
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PMC9429973
However, MYC is not therapeutically targetable due to its nuclear localization and the short half-life of the protein.
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PMC10761571
At first, the cells were treated with the nanoparticles for 24h, and then, were washed with PBS, and lysed.
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PMC11622247
In addition to their established role as hybridization reporters, the fluorescent base surrogates were hypothesized to function as smart acceptors of Förster resonance energy transfer (FRET) from bright Alexa Fluor (AF) dyes (568 or 488, Fig. 1B right).
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PMC4839679
Cells were lysed in cold Radio-Immunoprecipitation assay (RIPA) buffer containing freshly added protease inhibitor.
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PMC11700165
Based on cell lines and transgenic animal studies, the ITK-SYK chimera is known to be non-auto inhibited and therefore constitutively active.
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PMC9895440
b IFNγ secretion by ELISA of Teff and Tex in response to re-stimulation.
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PMC9429973
Aims: The aim of this study is to explore the role of DNMT3B in MM cell biology and drug response.
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PMC11507371
This anti-proliferative effect of CAP has been shown in multiple previous studies .
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PMC9429973
L. Busque, M. Harnois, N. Szuber, R. Delage, L. Mollica, H. Olney, P. Laneuville, S. Sirhan, G. Cournoyer, I. Chamakhi, M. Lalancette, D. Talbot, V. Éthier, P. Desjardins, S. Assouline Hematology, Hôpital Maisonneuve-Rosemont, Université de Montréal; Groupe Québécois de Recherche en LMC-NMP, GQR-LMC-NMP, Montreal; Hema...
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PMC9429973
Given it’s rarety, there is no specific protocol for staging and treatment.
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PMC11792888
As shown in Figure 7 A , the A549 cells incubated with etoposide showed a dose-dependent decrease in cell viability after 72 h of treatment.
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PMC7553912
The full-length blots are presented in Supplementary Fig. S6. (
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PMC11792090
The stability observed during scale-up production further supports their potential for clinical application.
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PMC11574271
Furthermore, our findings indicate that MSP activation of RON in 5637 cells leads to an upregulation of MMP12 expression (Fig. S1A), thereby enhancing cell migration and invasion activity.
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PMC9429973
The effect of GCs and glycolysis inhibitors on activated T cells was further explored in vitro and in a humanized murine model of aGvHD and graft versus leukaemia (GVL).
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PMC3681794
In p53 wild type U2OS cells p53 protein was readily detectable in untreated cells, suggesting protein stabilisation.
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PMC11711871
They exhibited increased adrenal soluble epoxide hydroxylase (sEH), favoring an increase in less active polyunsaturated fatty acids.
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PMC11015054
Subcutaneous tumorigenesis model using HepG2 cell line: (E, H) tumor volume, (F) tumor weight, and (G) tumor growth curve. (
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PMC10605143
Cells were cultured in Matrigel, as previously reported .
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PMC11001582
The presence of the targeted allele in the F1 generation was confirmed at the DNA level by the above PCR and Sanger sequencing.
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PMC11337594
Moreover, the percentage of apoptotic cells (Annexin V-positive) was reduced after CASP3 knockout (Fig. 5B and C).
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PMC11806818
These signaling molecules may not only be involved in the malignant transformation of hepatocytes, but may also be early diagnostic indicators of hepatocarcinogenesis or specific targeting molecules for hepatocellular carcinoma treatment.
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PMC11767817
However, it was observed that only compound 2a significantly increased the levels of proteins SIRT2 and HIF-1α, while compound 2a-B increased the expression of p38, p21, and HIF-2α.
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PMC8009078
Molecular docking is extremely successful to screen larger datasets of small molecular to achieve potential lead-like molecules for a specific target.
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PMC11453018
In conclusion, we have identified a novel mechanism by which EWS::FLI1 evades cellular senescence, pinpointing EWS:FLI1/P300/CBP as a vulnerable target in ES (Fig. 8).
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PMC11301242
Concurrently, the transcription levels of oncogenes within these regions also increased (Fig. 4A).Fig.
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PMC7504302
In MPM, Hoda et al. studied the dependency of MPM cell lines to activin receptor activity as well as the activins, members of TGF-β superfamily of secreted polypeptide growth factors.
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PMC10530622
Studies conducted on GO-deficient (Hao1 knockout) mouse models have demonstrated that elevated levels of glycolate do not cause any adverse effects.
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PMC9429973
In addition to the A, the number of P were significantly different between groups (p<0.01).
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PMC9920575
In the intrinsic pathway, p53 is related to cytochrome-c release from mitochondria.
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PMC10507284
Tropinin‐associated protein (TROAP), also known as tastin, is a cytoplasmic protein discovered in human epithelial cells by Fukuda et al. in 1995, which consists of 778 amino acid residues and contains potential protein kinase phosphorylation sites.
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PMC8998437
For copy number quantification, we applied standard curves based on serial dilutions of the plasmids with the inserted CCR1 or TBP transcripts derived from the established LCL-2mon reference line.
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PMC9429973
Patients with severe aGVRD presented more reactivation (73.5%/p-value 0.005) and also the unrelated transplant with MAC regimen (66.5%) or haploidentical (RIC 63%/MAC 59 %) with a low incidence in related transplant with RIC regimen (19.5%/p-value 0.001).
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PMC10523483
Histones are involved in regulating antioxidant factors that contribute to cell homeostasis and tumor progression; however, whether a major histone variant H1.2 plays roles in the development of NSCLC remains unclear.
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PMC11194678
Gels were stained by SYBR Gold (Life Technologies) and scanned by ChemiDoc XRS+ Gel Imaging System (Bio-Rad).
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PMC10523483
In Kras NSCLC model (31), knockout of H1.2 suppressed NSCLC progression and increased oxidative stress.
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PMC10577955
Apoptosis (programmed cell death) may be observed in both physiologically and pathologically mediated through two major pathways intrinsic and extrinsic; where the intrinsic pathway is specifically linked to mitochondrial outer membrane permeabilization and is activated by DNA damage, the mitochondrial pathway is based...
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PMC11637276
MDA-MB-231 cells, MDA-MB-231 cells overexpressing PPP2R1A-GFP, MDA-MB-231 cells expressing GFP, Telomerase immortalised normal fibroblasts (TIF) and SW1990 cells were cultured in high glucose Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS).
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PMC11033180
Loading were 15 μg of proteins for colon and brain tissues and 1/10 of culture well for cell cultures and gut explants.
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PMC11252033
The carbonyl oxygen atoms were found establishing a hydrogen bond with NH Val161.
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PMC10858941
In addition, hypoxia induces the activation of autophagy, which allows tumour cells to resist oxygen starvation stress .
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PMC9429973
MSI2 represents a potential novel target for therapeutic interventions in MCL.
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PMC8345486
Moreover, compound 23 enhanced the cisplatin effect in the spheroids, but this effect was additive rather than synergistic, indicating independent pathways of action of both compounds.
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PMC7154001
Metabolite profiling and identification studies of these compounds and currently used drugs are therefore essential.
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PMC10165258
On the right side of each plot, a boxplot represents the viability of the control group, subjected to either DMSO or NaCl.(D) Corresponding drug response curves obtained on spheroids in 96 well low-attachment plates.
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PMC9429973
Downstream ALK signaling cascades modulate both anti apoptotic signals and growth patterns fostering lymphoma proliferation and promoting an aggressive clinical behavior.
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PMC8735881
To facilitate the monitoring of transduced cells, GPI-10E8 was genetically linked to green fluorescent protein (GFP) through an internal 2A protein splicing signal in the lentiviral transfer vector pRRLsin-18.PPT.EF1α.
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PMC11139449
To overcome these challenges, we introduced a simple and cost-effective protocol based on the HITI-joined CRISPR strategy for genome editing of the CHO cell line.
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PMC10421378
Additionally, brightfield digital image processing parameters: Aspect Ratio and Area were used to characterize the shape and size of the analyzed bacterial cells in combination with the discrimination of bacterial cells from non-cellular debris (particles from prebiotic component) and single cells from aggregates.
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PMC11705862
Statistics (in B, D, F, H, J, and L): two-tailed student T-test.
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PMC6742971
Six to eight weeks old NSG-A2 mice (groups of five mice) were injected subcutaneously on the right flank with 2 × 10 human Me275 melanoma cells.
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PMC9429973
The second cohort (No. 2) tested by WT1 expression detection included 23 CML patients.
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PMC11790599
Future research should focus on identifying specific molecular targets of TSL, thereby increasing the chances of discovering novel druggable proteins for breast cancer therapeutics.
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PMC10578720
Moreover, molecular docking analysis confirmed that PGC-1α could directly interact with NF-κB p65 (Supplemental Figure S5, http://links.lww.com/HC9/A587).
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PMC7987994
Mg_HF_SPS sample was prepared by chemical treatment of magnesium powder in hydrofluoric acid (HF) for 24 h, cold pressing of this powder into a green compact, and subsequent spark plasma sintering at 500 °C.
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PMC11321678
We identified a total of 4526 genes that were uniquely active in each cluster (Figure 1F; and Table S3, Supporting Information).
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PMC11742231
This allows for a more comprehensive understanding of drug–cell interactions by considering both proximal and distant relationships within the molecular graph, improving prediction accuracy.
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PMC11538390
Two-sided P values are obtained from a likelihood ratio test in regression analysis and are not corrected for multiple comparisons.
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PMC11706297
The anticancer activity of BTT-1–10 was higher against lung cancer cells (Figure 2a) compared to that of BTP-1–10 (Figure 2b).
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PMC11190062
Thus, TLR activation may render MM cells less responsive to commonly used anti-myeloma drugs.
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PMC11643361
The cytotoxicity profiles were similar for these cell lines (Figure 4E).
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PMC11764090
This method is simple and easy to use.
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PMC11638255
Scale bar, 100 µm.
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PMC4695073
Since NER pathway consists of at least other five factors except XPA, we speculate that the effect of XPA on NER activity may also be restricted by other factors, especially while overexpressing.
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PMC11377827
Source data are available online for this figure. (
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PMC7338939
H&E staining for tumors in different groups.
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PMC9429973
Results: Overall survival was 44.1%.
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PMC9429973
Tumor response was observed in various subtypes, including AITL (13/20), PTCL-NOS (5/19), ALCL ALK- (2/4) and NKTCL (1/4).
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PMC10545062
Lastly, the NE titration scaling factors were adjusted to be more granular and reduce significant fluctuations in NE dosing and subsequent effects on systemic hemodynamics (Supplemental Digital Content, Figure 1, http://links.lww.com/TA/D127 and Supplemental Digital Content, Figure 3, http://links.lww.com/TA/D122).
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PMC11489275
These results suggested that the lower the level of peripheral blood lymphocytes, the higher the inflammatory factors, the higher the proportion of RP-ILD, and the higher the short-term mortality of patients.
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PMC11683240
STED images were taken using a custom-built dual-color three-dimensional STED microscope described in Osseforth et al. (63).
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PMC9250505
The half-maximal inhibitory concentration (IC50) of Olaparib (after 6 days) in EOC cells positively correlated with the levels of pBADS99 (Fig. 1F).
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PMC7762347
It is produced by many cyanobacterial genera, with Cylindrospermopsis raciborskii and Chrysosporum ovalisporum being its main producers .
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PMC10560648
Gene expression has also been a significant issue.
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PMC11578343
Unlike current rheological measurements, mz‐visco‐NPS allowed us to measure the high level of heterogeneity in viscoelasticity among single cells in a population.
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