PMCID
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PMC11701801
Data are presented as the mean ± SD; n = 3 independent experiments.
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PMC11607321
Multi-omic profiling is both costly and labor-intensive, thus data-driven generative models are key to prioritizing the design of the most informative experiments.
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PMC11224020
Data were analyzed by Kruskal–Wallis test; ****P < 0.0001; NS, P > 0.999.
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PMC7504302
Six kinase genes, namely, WEE1, AURKA, MPP3, MAP3K12, DGKD, and SPEG, scored for the most significant screening criteria.
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PMC11772585
After allowing the cells to adhere to the culture surface and incubating for 24 h, 2 × 10 cells were collected and centrifuged at 1000 rpm.
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PMC11127909
qRT-PCR was carried out by using SYBR Premix Ex Taq II reagents (TaKaRa), and the results were calculated based on the 2 method.
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PMC11409031
It is essential to perform further in vitro and in vivo studies to explore and validate the mechanism of action of the potential biomarker PFN1 in ovarian cancer.
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PMC10761571
Table 1PrimerSequence (5′-3′)Product size (bp)ReferenceBax-forwardTTGCTTCAGGGTTTCATCCA113Bax-reverseAGACACTCGCTCAGCTTCTTGCASP8-forwardGACTGGATTTGCTGATTACCTACCTAA143CASP8-reverseCCTCAATTCTGATCTGCTCACTTCTBcl2-forwardTGGCCAGGGTCAGAGTTAAA147Bcl2-reverseTGGCCTCTCTTGCGGAGTAGAPDH-forwardCCCACTCCTCCACCTTTGAC75GAPDH-reverseCATA...
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PMC11766332
Recent classification has accounted for 189 CDG-related gene defects resulting in almost 200 distinct phenotypes .
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PMC10936243
This mixed-methods study used an explanatory sequential design that firstly involved using quantitative methods, followed by qualitative methods (see Figure 1).
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PMC6181431
Furthermore, tumor heterogeneity is well-known in RCC [12,25–27], and complicates interpretation of genetic differences seen in the two paired tumor samples.
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PMC11470381
Utilize Fill Holes and Analyze Particles while specifying the maximum and minimum cell area to generate outlines of ROIs corresponding to each cell mask.
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PMC6450504
The PTX IC50 of 0.5%PEIPOS was 2.4 nM compared to 2.5 nM for free PTX.
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PMC11738017
To evaluate whether sBCMA affects the cytotoxic activity of anti-BCMA CAR T therapy, we generated anti-BCMA CAR Ts with 3 distinct BCMA binding domains, namely CAR T, CAR T, and CAR T. CAR T and CAR T were generated by cloning the anti-BCMA single-chain variable fragment (scFv) sequences of idecabtagene vicleucel and c...
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PMC9429973
HU-induced citrulline levels can be reduced by treatment with the NOS inhibitor L-NAME, indicating that NO is produced de novo by the NOS enzyme rather than HU degradation.
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PMC10761218
The IC50 values for the methanolic extracts of W. somnifera leaf and stem were determined to be 43.06 ± 0.615 μg/mL and 45.60 ± 0.3 μg/mL, respectively, in HepG2 cell lines.
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PMC11640419
The cytotoxic effect of the synthesized compounds and the original oleanolic acid on the selected cell lines, with a few exceptions, increased in a predictable manner.
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PMC11477621
Compound 5i was prepared using similar procedures as for 3a from compound 4i (499 mg, 1.71 mmol).
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PMC11422497
P < 0.05, **P < 0.01.
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PMC11661024
Finally, the outcomes were reported as the half-maximal inhibitory concentration (IC50) values and the viability percentage.
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PMC11754436
The Tunel assay was used to successfully detect apoptosis in MG63 cells after co-transfection with miR-362-5p mimetics and NPEPPS. (
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PMC11742290
Trichosanthin (TCS), a bioactive protein extracted and purified from the tuberous root of Trichosanthes kirilowii (a well‑known traditional Chinese medicinal plant), induced apoptosis also by targeting LGR5 and repressing the WNT/β‑catenin signaling pathway in glioma cells .
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PMC9429973
NK cells expressing the CAR containing the shorter linker between the scFv and the transmembrane domain exhibited higher activation levels.
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PMC8409415
B, IC50 values for cisplatin were determined in A2780cisR and SKOV3cisR cells.
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PMC9429973
The association between blood counts and TEs was assessed using a time-dependent covariate Cox proportional hazards model.
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PMC9429973
Results: The expression of SHP-1 was reduced by 30% at the RNA level and 50% at the protein level in UT-7 cells transduced with JAK2V617F compared to JAK2wt -transduced cells.
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PMC9429973
Trogocytosis assay: ICTs were co-cultured with stained additional autologous AML cells for 1 h and T cell populations of interest were isolated using FACS.
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PMC11502327
Summary of experimental data on the mechanism of action of natural drug ingredients on OC.
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PMC11798926
The surface localization of the mutant SynDIG4 enabled us to visualize co-localization of SynDIG4 and ionotropic glutamate receptors on the surface of transfected cells (Figure 4).
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PMC7840816
It has been reported that SOX9 regulated cell proliferation and cell invasion by the Wnt/β-catenin signaling pathway, so we investigated the effects of SOX9 on the downstream target genes of the Wnt pathway, β-catenin, cyclin D1, and c-Myc.
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PMC9429973
This can be explained by IM inhibition of non-mutated BCR::ABL1 in KCL-22IR carrying 2 Ph chromosomes.
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PMC11792888
No. 9212, 1:1000), phospho-p38 MAPK (Thr180/Tyr182) (CST, Cat.
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PMC7802142
Fig. 7ReN-NV promote β-catenin signaling pathway through miR-100.
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PMC9429973
Overall 279 neutropenic patients were enrolled in the studies and written informed consent has been given.
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PMC11610461
Next, 1 mmol and 10 mmol of metformin media were added to the wells, and the wells without metformin medium served as controls.
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PMC10135767
The cells were resuspended in 2% BSA (w/v) (Sigma, Burlington, MA, USA) and incubated for 30 min.
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PMC11672617
The data were processed using the NovoExpress software (version 1.6.2) (ACEA (Agilent, Santa Clara, CA, USA)).
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PMC9985266
Del Principe et al. found that autophagy deficiency promotes ECM deposition in lung fibroblasts and accelerates the fibrosis process.
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PMC10218459
The plasma flame was guided over a 24-well plate containing 2 × 10 cells per 200 µL of full medium for 5, 10, and 20 s, while a control treatment with argon was also performed.
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PMC11599565
However, this “neuronal” background and the more defined neuron-like signature caused by cisplatin exposure probably do not render in functional neurons at this stage, since preliminary experiments for the excitatory physiology of mature neurons were unsuccessful (data not shown).
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PMC10142392
Most cancer cells are found in an acidic environment, which stimulates the development of pH-sensitive drug delivery systems.
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PMC8389560
This leads to the activation of vesicular release of ATP through connexin hemichannels (Cx43) and paracrine cell activation through purinergic receptors (mainly P2Y).
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PMC11098378
C. Schematic outlining UBAIT protocol .
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PMC3765139
Tavaluc and Zhou suggest that CSCs have a higher ability to survive under hypoxic conditions than normal cancer cells.
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PMC11604015
A: Knocking out target antigens (such as CD7) through CRISPR/Cas9 or base editing in CAR-T cells can prevent fratricide maximally.
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PMC11448304
Table S4 lists the amino acid residues from -10 to + 10 surrounding the Khib site, and the Top 5 overrepresented patterns are shown in Fig. 5.Fig.
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PMC10253553
The cells (786-0) were transfected with siRNAs (100 nM) and plated in 96-well plates at 5000 cells/well.
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PMC9727330
Then, 100 µl of the lysed cells were transferred into the ELISA plate reader and incubated for 2 hrs R.T. with 100 µl control solution and 50µl 1X biotinylated antibody.
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PMC7917457
The luciferase activity was determined with Varioskan Flash (Thermo Labsystems).
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PMC11634027
Examination of Ds:V5 protein expressed by ds revealed a distribution similar to that previously described for Ds, including a proximal-to-distal gradient with relatively high levels in the wing hinge, low levels in the proximal wing pouch, and barely detectable levels in the distal wing pouch (Fig. 3A).
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PMC11634027
Clustal Omega (https://www.ebi.ac.uk/jdispatcher/msa/clustalo) was used to identify sequence conservation among Dachsous ICDs from different species (Madeira et al., 2022), which was then displayed using Jalview (https://www.jalview.org) (Waterhouse et al., 2009).
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PMC9429973
The disease was localized in 31%.
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PMC11467964
At the same time, the current clinical research is exploring the potential clinical value of trabectedin in the treatment of STS.
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PMC9275501
In vitro and in vivo experiments showed (DOX + CUR)-FA-NPs not only had remarkable tumor targeting and anticancer efficacy, but also had less side effects on normal tissues (Guo et al., 2021).
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PMC11705862
Before assessing the binding, the cells were stimulated with high potassium concentrations, mimicking the evoked secretory state.
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PMC11063646
The obtained non-significant lack of fit was good for the model to fit in the design space (Table 4 &Table 5) Quadratic/ Linear equations were generated by Design expert software, based on the responses obtained (Table 7) for the prepared emulgels (ANG1-ANG 11).
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PMC8584666
To estimate the effect of oxidation induced by GPI-80 expression, the authors investigated whether GPI-80 expression is involved in NF-κB activation.
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PMC11451940
IR (ATR; cm): 3068, 3021, 2958, 2909, 2852, 1647, 1588, 1541, 1424, 1369, 1350, 1269, 1238, 1221, 951, 880, 807, 731, 698, 664, 552, 436.
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PMC11188874
Cells were transfected with a plasmid that expressed full-length, WT FLAG-tagged HDAC6, treated with HDA6 inhibitors (1 µmol/L) for 24 hours, lysates prepared and separated by SDS-PAGE.
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PMC10761218
The seeds were sown and matured under natural soil and light conditions at the Department of Genetics and Biotechnology, Osmania University, Hyderabad.
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PMC11727145
Laminin 1, primarily located in the skin basement membrane, plays a crucial role in cellular adhesion and migration .
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PMC9429973
Through cycle 9, the completion rate among patients treated at each cycle was ≥ 92% for EQ-5D and ≥ 88% for FACT-Lym.
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PMC11718817
We hypothesize that molecular interventions such as treatment of macrophages with lipopolysaccharide (LPS) and nigericin for NLRP3 activation significantly affect T cell migration within spheroids.
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PMC9960565
The solution was left for 40 h at r.t.
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PMC9429973
Physicians highlight an urgent need for well-tolerated therapies that can tackle organ involvement, with less frequent adverse effects and less severe toxicity.
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PMC11590870
The farm has sandy loam soil, which was irrigated at regular intervals as required.
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PMC11352746
Compared to other tissues and cells, the regenerative capacity of neurons in the central nervous system is restricted; hence, their difficulty in repairing and regenerating after damage .
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PMC5417661
Cells were then seeded into the drug-prepared plates that were placed in an incubator for 72 h. On the last day of incubation, the plates were washed, and a buffer containing fluorescein diacetate was added and incubated with the cells for 45 min.
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PMC4485786
Multinucleate, senescent A204 cells and expression of p21 and myogenic markers in native and cytokine-treated A204 cells. (
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PMC10965680
The Xiangdong goat has a moderate level of genetic diversity, and the decreased LD decay and inbreeding levels revealed by ROH and effective population size make it a viable genetic resource.
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PMC11400680
For the nucleus segmentation (Table 5), our mAP50 score for easy test set (68.04%) is lower than that of DeepCeNS (74.43%).
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PMC11718274
The results of the regression analysis shown in Fig. 10 evidenced the strong dependence of gene expression on Y2O3NPs concentrations where a strong negative correlation (r= -0.98) was detected between Bcl2 gene expression and the concentrations of Y2O3NPs tested, and a strong positive correlation was reported between Y...
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PMC10033453
We hypothesize that variability in viral distribution within the tumor, model heterogeneity, immune infiltration, and IFN sensitivity of the model could all contribute to the varied responses, among others.
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PMC9429973
Methods: This is a retrospective unicenter study that included all adult patients with hematologic diseases that required a PICC insertion between June 2019 and December 2021.
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PMC11295144
Quantification of the % area of co-localized VGAT and GFP pixels that represent contacts showed potentiation of NL2 effects by α2β2γ2-GABAARs irrespective of whether the cultures were incubated with DMSO (control HEK293/NL2 cells: median = 1.68%; IQR = 0.54 – 2.57%; n = 20; α2β2γ2-GABAAR/NL2 cells: median = 4.21%; IQR ...
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PMC11746942
The animal experiment was approved by Nanjing University Ethics Committee.
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PMC11310713
Our study demonstrates the efficacy of an injectable cell-based HRT using isolated ovarian cell-embedded hydrogel beads, which eliminates the need for transplantation.
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PMC7348793
The third conclusion is that a comparison of the spectra of PCR products generated of the total DNA isolated from the cell lines (Figure 1, lanes 2–4 and 9–11) with those generated on chromosomal/plasmid DNA mixtures (Figure 1, lanes 5–7 and 12–14) clearly indicate the mtDNA control region length heterogeneity in all t...
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PMC4839679
Using a ccRCC with a loss-of-function PBRM1 mutation, we found that re-expression of PBRM1 reverses some of the metabolic effects characteristic of ccRCC, including increased glucose uptake, shifts in cholesterol esters, and increased insulin/PI3K signaling.
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PMC8875242
Values differing from controls at the p < 0.05, p < 0.01, and p < 0.001 levels are indicated with the labels *, **, and ***, respectively.
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PMC10582049
Second, the possible biological mechanisms that determine the presence of different CAFs subpopulations in the LUAD tissue were not investigated.
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PMC10671321
The hiPSC line was named REGUi009-A in agreement with the European nomenclature of the human pluripotent stem cell registry (hPSCreg).
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PMC7215722
The ER is the most critical organelle in which protein trafficking is regulated in its early stages.
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PMC9739791
Interestingly, the Pt(IV) prodrug 23 with 3 freely rotating CH2 links showed the highest activation rate, while both Pt(IV) prodrugs with two freely rotating CH2 bonds 17 and 18 demonstrated the slowest reduction rate.
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PMC10243817
The diagnosis of CRSsNP and CRSwNP was made according to the European Position Paper on Rhinosinusitis and Nasal Polyps 2020 (14).
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PMC9541067
a Transwell cell migration assay was performed after the overexpression in OSRC-2 and A498 cells.
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PMC11805741
Generally, the effect of the imbalanced expression of proapoptotic and pro survival modulator of BCL-2 family proteins on tumor development and the resistance of malignant cells to anti-cancer agents is well studied.
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PMC11791203
For calculation of percentage mortality, the viability of cells grown in medium containing vehicle alone (0.1% DMSO) was considered as 100%.
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PMC11040965
Given that abnormal miR29b expression patterns have been reported in various tumor cells (reviewed in ), miR29b is considered to be an epigenetic controller (epi-miRNA) that is involved in tumor development and progression .
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PMC9739791
Thus, under green light irradiation, BODI-Pt could induce cell death via oncosis through the release of Pt(II) complex that binds with DNA and ROS generation.
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PMC11615218
A study utilizing TCR sequencing and CITE-seq/transcriptome analysis in MM showed that greater baseline TCR diversity was associated with longer PFS, while increased clonal expansion of terminally differentiated T cell clones correlated with shorter PFS .
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PMC11045125
Briefly, tumor cell lysates were prepared in TRIzol (Invitrogen) after powdering the tissue in liquid Nitrogen from tissue preserved on dry ice/stored at -80°C.
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PMC11730305
Cobalt oxide nanoparticles have been used in various applications such as energy storage, photocatalysis, rechargeable ion batteries, electrochromic sensors, and solar cells.
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PMC11019355
Further, nearly half of trans elements carry a footprint of TFs differentially expressed in human and rhesus lymphoblastoid cell lines, revealing only a handful of key immune regulators as driving a majority of trans species differences.
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PMC11252553
A significance level of P<0.005 was used to determine statistical significance in this analysis.
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PMC9105697
The sequence reads of cfDNA samples containing UMIs were uploaded to the Curio Genomics web platform (https://curiogenomics.com, (accessed on 13 January 2020)) and aligned to GRChg38 using Bowtie2.
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PMC9429973
However, it is not clear to what degree this T cell response targets AML neo-antigens.
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PMC4485648
This pathway is usually downregulated by hypoxia (41,42), so it is plausible that it would be upregulated with the decrease of the hypoxic signaling.
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PMC10329237
More importantly, this drug is widely used to treat the current global SARS-CoV-2 pandemic (7).
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PMC11703992
Notably, CD4+ Memory T cells showed an individual differentiation branch.
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