PMCID string | Sentences string | ner list |
|---|---|---|
PMC11701801 | Data are presented as the mean ± SD; n = 3 independent experiments. | [
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PMC11607321 | Multi-omic profiling is both costly and labor-intensive, thus data-driven generative models are key to prioritizing the design of the most informative experiments. | [
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PMC11224020 | Data were analyzed by Kruskal–Wallis test; ****P < 0.0001; NS, P > 0.999. | [
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PMC7504302 | Six kinase genes, namely, WEE1, AURKA, MPP3, MAP3K12, DGKD, and SPEG, scored for the most significant screening criteria. | [
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PMC11772585 | After allowing the cells to adhere to the culture surface and incubating for 24 h, 2 × 10 cells were collected and centrifuged at 1000 rpm. | [
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PMC11127909 | qRT-PCR was carried out by using SYBR Premix Ex Taq II reagents (TaKaRa), and the results were calculated based on the 2 method. | [
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PMC11409031 | It is essential to perform further in vitro and in vivo studies to explore and validate the mechanism of action of the potential biomarker PFN1 in ovarian cancer. | [
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PMC10761571 | Table 1PrimerSequence (5′-3′)Product size (bp)ReferenceBax-forwardTTGCTTCAGGGTTTCATCCA113Bax-reverseAGACACTCGCTCAGCTTCTTGCASP8-forwardGACTGGATTTGCTGATTACCTACCTAA143CASP8-reverseCCTCAATTCTGATCTGCTCACTTCTBcl2-forwardTGGCCAGGGTCAGAGTTAAA147Bcl2-reverseTGGCCTCTCTTGCGGAGTAGAPDH-forwardCCCACTCCTCCACCTTTGAC75GAPDH-reverseCATA... | [
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PMC11766332 | Recent classification has accounted for 189 CDG-related gene defects resulting in almost 200 distinct phenotypes . | [
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PMC10936243 | This mixed-methods study used an explanatory sequential design that firstly involved using quantitative methods, followed by qualitative methods (see Figure 1). | [
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PMC6181431 | Furthermore, tumor heterogeneity is well-known in RCC [12,25–27], and complicates interpretation of genetic differences seen in the two paired tumor samples. | [
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PMC11470381 | Utilize Fill Holes and Analyze Particles while specifying the maximum and minimum cell area to generate outlines of ROIs corresponding to each cell mask. | [
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PMC6450504 | The PTX IC50 of 0.5%PEIPOS was 2.4 nM compared to 2.5 nM for free PTX. | [
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PMC11738017 | To evaluate whether sBCMA affects the cytotoxic activity of anti-BCMA CAR T therapy, we generated anti-BCMA CAR Ts with 3 distinct BCMA binding domains, namely CAR T, CAR T, and CAR T. CAR T and CAR T were generated by cloning the anti-BCMA single-chain variable fragment (scFv) sequences of idecabtagene vicleucel and c... | [
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PMC9429973 | HU-induced citrulline levels can be reduced by treatment with the NOS inhibitor L-NAME, indicating that NO is produced de novo by the NOS enzyme rather than HU degradation. | [
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PMC10761218 | The IC50 values for the methanolic extracts of W. somnifera leaf and stem were determined to be 43.06 ± 0.615 μg/mL and 45.60 ± 0.3 μg/mL, respectively, in HepG2 cell lines. | [
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PMC11640419 | The cytotoxic effect of the synthesized compounds and the original oleanolic acid on the selected cell lines, with a few exceptions, increased in a predictable manner. | [
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PMC11477621 | Compound 5i was prepared using similar procedures as for 3a from compound 4i (499 mg, 1.71 mmol). | [
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PMC11422497 | P < 0.05, **P < 0.01. | [
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PMC11661024 | Finally, the outcomes were reported as the half-maximal inhibitory concentration (IC50) values and the viability percentage. | [
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PMC11754436 | The Tunel assay was used to successfully detect apoptosis in MG63 cells after co-transfection with miR-362-5p mimetics and NPEPPS. ( | [
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PMC11742290 | Trichosanthin (TCS), a bioactive protein extracted and purified from the tuberous root of Trichosanthes kirilowii (a well‑known traditional Chinese medicinal plant), induced apoptosis also by targeting LGR5 and repressing the WNT/β‑catenin signaling pathway in glioma cells . | [
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PMC9429973 | NK cells expressing the CAR containing the shorter linker between the scFv and the transmembrane domain exhibited higher activation levels. | [
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PMC8409415 | B, IC50 values for cisplatin were determined in A2780cisR and SKOV3cisR cells. | [
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PMC9429973 | The association between blood counts and TEs was assessed using a time-dependent covariate Cox proportional hazards model. | [
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PMC9429973 | Results: The expression of SHP-1 was reduced by 30% at the RNA level and 50% at the protein level in UT-7 cells transduced with JAK2V617F compared to JAK2wt -transduced cells. | [
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PMC9429973 | Trogocytosis assay: ICTs were co-cultured with stained additional autologous AML cells for 1 h and T cell populations of interest were isolated using FACS. | [
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PMC11502327 | Summary of experimental data on the mechanism of action of natural drug ingredients on OC. | [
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PMC11798926 | The surface localization of the mutant SynDIG4 enabled us to visualize co-localization of SynDIG4 and ionotropic glutamate receptors on the surface of transfected cells (Figure 4). | [
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PMC7840816 | It has been reported that SOX9 regulated cell proliferation and cell invasion by the Wnt/β-catenin signaling pathway, so we investigated the effects of SOX9 on the downstream target genes of the Wnt pathway, β-catenin, cyclin D1, and c-Myc. | [
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PMC9429973 | This can be explained by IM inhibition of non-mutated BCR::ABL1 in KCL-22IR carrying 2 Ph chromosomes. | [
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PMC11792888 | No. 9212, 1:1000), phospho-p38 MAPK (Thr180/Tyr182) (CST, Cat. | [
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PMC7802142 | Fig. 7ReN-NV promote β-catenin signaling pathway through miR-100. | [
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PMC9429973 | Overall 279 neutropenic patients were enrolled in the studies and written informed consent has been given. | [
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PMC11610461 | Next, 1 mmol and 10 mmol of metformin media were added to the wells, and the wells without metformin medium served as controls. | [
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PMC10135767 | The cells were resuspended in 2% BSA (w/v) (Sigma, Burlington, MA, USA) and incubated for 30 min. | [
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PMC11672617 | The data were processed using the NovoExpress software (version 1.6.2) (ACEA (Agilent, Santa Clara, CA, USA)). | [
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PMC9985266 | Del Principe et al. found that autophagy deficiency promotes ECM deposition in lung fibroblasts and accelerates the fibrosis process. | [
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PMC10218459 | The plasma flame was guided over a 24-well plate containing 2 × 10 cells per 200 µL of full medium for 5, 10, and 20 s, while a control treatment with argon was also performed. | [
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PMC11599565 | However, this “neuronal” background and the more defined neuron-like signature caused by cisplatin exposure probably do not render in functional neurons at this stage, since preliminary experiments for the excitatory physiology of mature neurons were unsuccessful (data not shown). | [
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PMC10142392 | Most cancer cells are found in an acidic environment, which stimulates the development of pH-sensitive drug delivery systems. | [
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PMC8389560 | This leads to the activation of vesicular release of ATP through connexin hemichannels (Cx43) and paracrine cell activation through purinergic receptors (mainly P2Y). | [
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PMC11098378 | C. Schematic outlining UBAIT protocol . | [
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PMC3765139 | Tavaluc and Zhou suggest that CSCs have a higher ability to survive under hypoxic conditions than normal cancer cells. | [
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PMC11604015 | A: Knocking out target antigens (such as CD7) through CRISPR/Cas9 or base editing in CAR-T cells can prevent fratricide maximally. | [
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PMC11448304 | Table S4 lists the amino acid residues from -10 to + 10 surrounding the Khib site, and the Top 5 overrepresented patterns are shown in Fig. 5.Fig. | [
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PMC10253553 | The cells (786-0) were transfected with siRNAs (100 nM) and plated in 96-well plates at 5000 cells/well. | [
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PMC9727330 | Then, 100 µl of the lysed cells were transferred into the ELISA plate reader and incubated for 2 hrs R.T. with 100 µl control solution and 50µl 1X biotinylated antibody. | [
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PMC7917457 | The luciferase activity was determined with Varioskan Flash (Thermo Labsystems). | [
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PMC11634027 | Examination of Ds:V5 protein expressed by ds revealed a distribution similar to that previously described for Ds, including a proximal-to-distal gradient with relatively high levels in the wing hinge, low levels in the proximal wing pouch, and barely detectable levels in the distal wing pouch (Fig. 3A). | [
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PMC11634027 | Clustal Omega (https://www.ebi.ac.uk/jdispatcher/msa/clustalo) was used to identify sequence conservation among Dachsous ICDs from different species (Madeira et al., 2022), which was then displayed using Jalview (https://www.jalview.org) (Waterhouse et al., 2009). | [
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PMC9429973 | The disease was localized in 31%. | [
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PMC11467964 | At the same time, the current clinical research is exploring the potential clinical value of trabectedin in the treatment of STS. | [
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PMC9275501 | In vitro and in vivo experiments showed (DOX + CUR)-FA-NPs not only had remarkable tumor targeting and anticancer efficacy, but also had less side effects on normal tissues (Guo et al., 2021). | [
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PMC11705862 | Before assessing the binding, the cells were stimulated with high potassium concentrations, mimicking the evoked secretory state. | [
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PMC11063646 | The obtained non-significant lack of fit was good for the model to fit in the design space (Table 4 &Table 5) Quadratic/ Linear equations were generated by Design expert software, based on the responses obtained (Table 7) for the prepared emulgels (ANG1-ANG 11). | [
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PMC8584666 | To estimate the effect of oxidation induced by GPI-80 expression, the authors investigated whether GPI-80 expression is involved in NF-κB activation. | [
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PMC11451940 | IR (ATR; cm): 3068, 3021, 2958, 2909, 2852, 1647, 1588, 1541, 1424, 1369, 1350, 1269, 1238, 1221, 951, 880, 807, 731, 698, 664, 552, 436. | [
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PMC11188874 | Cells were transfected with a plasmid that expressed full-length, WT FLAG-tagged HDAC6, treated with HDA6 inhibitors (1 µmol/L) for 24 hours, lysates prepared and separated by SDS-PAGE. | [
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PMC10761218 | The seeds were sown and matured under natural soil and light conditions at the Department of Genetics and Biotechnology, Osmania University, Hyderabad. | [
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PMC11727145 | Laminin 1, primarily located in the skin basement membrane, plays a crucial role in cellular adhesion and migration . | [
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PMC9429973 | Through cycle 9, the completion rate among patients treated at each cycle was ≥ 92% for EQ-5D and ≥ 88% for FACT-Lym. | [
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PMC11718817 | We hypothesize that molecular interventions such as treatment of macrophages with lipopolysaccharide (LPS) and nigericin for NLRP3 activation significantly affect T cell migration within spheroids. | [
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PMC9960565 | The solution was left for 40 h at r.t. | [
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PMC9429973 | Physicians highlight an urgent need for well-tolerated therapies that can tackle organ involvement, with less frequent adverse effects and less severe toxicity. | [
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PMC11590870 | The farm has sandy loam soil, which was irrigated at regular intervals as required. | [
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PMC11352746 | Compared to other tissues and cells, the regenerative capacity of neurons in the central nervous system is restricted; hence, their difficulty in repairing and regenerating after damage . | [
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PMC5417661 | Cells were then seeded into the drug-prepared plates that were placed in an incubator for 72 h. On the last day of incubation, the plates were washed, and a buffer containing fluorescein diacetate was added and incubated with the cells for 45 min. | [
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PMC4485786 | Multinucleate, senescent A204 cells and expression of p21 and myogenic markers in native and cytokine-treated A204 cells. ( | [
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PMC10965680 | The Xiangdong goat has a moderate level of genetic diversity, and the decreased LD decay and inbreeding levels revealed by ROH and effective population size make it a viable genetic resource. | [
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PMC11400680 | For the nucleus segmentation (Table 5), our mAP50 score for easy test set (68.04%) is lower than that of DeepCeNS (74.43%). | [
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PMC11718274 | The results of the regression analysis shown in Fig. 10 evidenced the strong dependence of gene expression on Y2O3NPs concentrations where a strong negative correlation (r= -0.98) was detected between Bcl2 gene expression and the concentrations of Y2O3NPs tested, and a strong positive correlation was reported between Y... | [
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PMC10033453 | We hypothesize that variability in viral distribution within the tumor, model heterogeneity, immune infiltration, and IFN sensitivity of the model could all contribute to the varied responses, among others. | [
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PMC9429973 | Methods: This is a retrospective unicenter study that included all adult patients with hematologic diseases that required a PICC insertion between June 2019 and December 2021. | [
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PMC11295144 | Quantification of the % area of co-localized VGAT and GFP pixels that represent contacts showed potentiation of NL2 effects by α2β2γ2-GABAARs irrespective of whether the cultures were incubated with DMSO (control HEK293/NL2 cells: median = 1.68%; IQR = 0.54 – 2.57%; n = 20; α2β2γ2-GABAAR/NL2 cells: median = 4.21%; IQR ... | [
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PMC11746942 | The animal experiment was approved by Nanjing University Ethics Committee. | [
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PMC11310713 | Our study demonstrates the efficacy of an injectable cell-based HRT using isolated ovarian cell-embedded hydrogel beads, which eliminates the need for transplantation. | [
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PMC7348793 | The third conclusion is that a comparison of the spectra of PCR products generated of the total DNA isolated from the cell lines (Figure 1, lanes 2–4 and 9–11) with those generated on chromosomal/plasmid DNA mixtures (Figure 1, lanes 5–7 and 12–14) clearly indicate the mtDNA control region length heterogeneity in all t... | [
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PMC4839679 | Using a ccRCC with a loss-of-function PBRM1 mutation, we found that re-expression of PBRM1 reverses some of the metabolic effects characteristic of ccRCC, including increased glucose uptake, shifts in cholesterol esters, and increased insulin/PI3K signaling. | [
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PMC8875242 | Values differing from controls at the p < 0.05, p < 0.01, and p < 0.001 levels are indicated with the labels *, **, and ***, respectively. | [
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PMC10582049 | Second, the possible biological mechanisms that determine the presence of different CAFs subpopulations in the LUAD tissue were not investigated. | [
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PMC10671321 | The hiPSC line was named REGUi009-A in agreement with the European nomenclature of the human pluripotent stem cell registry (hPSCreg). | [
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PMC7215722 | The ER is the most critical organelle in which protein trafficking is regulated in its early stages. | [
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PMC9739791 | Interestingly, the Pt(IV) prodrug 23 with 3 freely rotating CH2 links showed the highest activation rate, while both Pt(IV) prodrugs with two freely rotating CH2 bonds 17 and 18 demonstrated the slowest reduction rate. | [
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PMC10243817 | The diagnosis of CRSsNP and CRSwNP was made according to the European Position Paper on Rhinosinusitis and Nasal Polyps 2020 (14). | [
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PMC9541067 | a Transwell cell migration assay was performed after the overexpression in OSRC-2 and A498 cells. | [
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PMC11805741 | Generally, the effect of the imbalanced expression of proapoptotic and pro survival modulator of BCL-2 family proteins on tumor development and the resistance of malignant cells to anti-cancer agents is well studied. | [
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PMC11791203 | For calculation of percentage mortality, the viability of cells grown in medium containing vehicle alone (0.1% DMSO) was considered as 100%. | [
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PMC11040965 | Given that abnormal miR29b expression patterns have been reported in various tumor cells (reviewed in ), miR29b is considered to be an epigenetic controller (epi-miRNA) that is involved in tumor development and progression . | [
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PMC9739791 | Thus, under green light irradiation, BODI-Pt could induce cell death via oncosis through the release of Pt(II) complex that binds with DNA and ROS generation. | [
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PMC11615218 | A study utilizing TCR sequencing and CITE-seq/transcriptome analysis in MM showed that greater baseline TCR diversity was associated with longer PFS, while increased clonal expansion of terminally differentiated T cell clones correlated with shorter PFS . | [
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PMC11045125 | Briefly, tumor cell lysates were prepared in TRIzol (Invitrogen) after powdering the tissue in liquid Nitrogen from tissue preserved on dry ice/stored at -80°C. | [
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PMC11730305 | Cobalt oxide nanoparticles have been used in various applications such as energy storage, photocatalysis, rechargeable ion batteries, electrochromic sensors, and solar cells. | [
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PMC11019355 | Further, nearly half of trans elements carry a footprint of TFs differentially expressed in human and rhesus lymphoblastoid cell lines, revealing only a handful of key immune regulators as driving a majority of trans species differences. | [
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PMC11252553 | A significance level of P<0.005 was used to determine statistical significance in this analysis. | [
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PMC9105697 | The sequence reads of cfDNA samples containing UMIs were uploaded to the Curio Genomics web platform (https://curiogenomics.com, (accessed on 13 January 2020)) and aligned to GRChg38 using Bowtie2. | [
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... |
PMC9429973 | However, it is not clear to what degree this T cell response targets AML neo-antigens. | [
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PMC4485648 | This pathway is usually downregulated by hypoxia (41,42), so it is plausible that it would be upregulated with the decrease of the hypoxic signaling. | [
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PMC10329237 | More importantly, this drug is widely used to treat the current global SARS-CoV-2 pandemic (7). | [
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PMC11703992 | Notably, CD4+ Memory T cells showed an individual differentiation branch. | [
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