PMCID
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PMC11350568
Comparison of the binding ability of aptamers against MV-4–11 cells.
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PMC11040965
P < .05, Wilcoxon signed-rank test We used the same assay to assess whether CD28 engagement influences levels of miR29b and its target DNMT3B in U266 and RPMI 8226 myeloma cells (Fig. 1C-E).
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PMC8708099
The symptoms of the disease can be reduced by using appropriate pharmacological agents, but IBD is not fully curable .
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PMC9847912
Titers produced by transfecting the M3 clone into 293T/17 cells (as measured by plating 293T/17 supernatants onto MAGIC-5A cells) were 25% of those produced by the parental HIV-2ROD9 plasmid, indicating a substantial fitness cost.
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PMC11577421
in handling more heterogeneous size distributions.
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PMC11574271
In summary, the current research has revealed that decreased expression of has-mir-659-3p plays a significant role in the upregulation of RON in bladder cancer.
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PMC11536589
Then, we utilized the R package “monocle” to compute and visualize the pseudo-time trajectories of all KPC cells.
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PMC5916734
The copy numbers of the auxiliary genes in all the generated clonal cell lines were significantly lower than that of the FIX gene, because cell clones were not selected in accordance with the maximum activity of the vkorc1 gene.
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PMC11607638
The animal protocol used in this work to isolate pancreatic acini from male rats was approved by The Animal Use and Ethics Committee, College for Life Sciences, Beijing Normal University (No. CLS‐EAW‐2017‐015).
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PMC11607638
All experimental data were processed and analyzed with GraphPad Prism (RRID:SCR‐002798) 8.4.0.
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PMC9268620
The same observations were also found in the treatments with 1 and 3 μg/mL of nordamnacanthal, showing that the compound at low concentrations did not affect the cells.
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PMC11747949
Gathered supernatant was centrifuged again 1000 × g for 5 min, and prepared for ELISA analysis.
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PMC9429973
N. Jain, P. Thompson, J. Burger, A. Ferrajoli, K. Takahashi, Z. Estrov, G. Borthakur, P. Bose, T. Kadia, N. Pemmaraju, K. Sasaki, M. Konopleva, E. Jabbour, N. Garg, X. Wang, R. Kanagal-Shamanna, K. Patel, W. Wang, S. Wang, J. Jorgensen, W. Lopez, A. Ayala, W. Plunkett, V. Gandhi, H. Kantarjian, S. O’Brien, M. Keating, ...
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PMC11629143
ChIP‐qPCR and RT‐qPCR primer sequences.
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PMC11487720
In contrast, when WWOX-deficient or -dysfunctional (WWOXd) cells are exposed to UV and/or cold shock, the cells undergo nuclear pop-out explosion death (POD).
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PMC8068766
Therefore, it is essential to determine the stress response gene expression profiles of these cells due to their involvement in hepatocarcinogenesis (Di Maso et al., 2015).
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PMC10094712
hSELENOM penetrates into cells, most likely via actin-dependent endocytosis as shown in this work (Figure 8), and interacts with reduced glutathione due to the presence of the CXCS active center (where C is cysteine and X is any amino acid).
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PMC11684269
PD-1 facilitates the metabolic rewiring of activated T cells from glycolysis to FAO by upregulating CPT1A, thus exerting the immunosuppressive capacity (54).
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PMC11116779
Particles sized less than 10 μm enter the lungs and can reach the alveolar cells, which accumulate these air pollutants over a lifetime (19).
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PMC9813422
The HPV E2 protein binds viral episomes to mitotic host chromosomes during cell division for partitioning and maintenance.
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PMC9429973
Our nomogram may help clinicians in clinical decision making.
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PMC11740399
Lipids are transported from the liver to peripheral tissues or back to the liver through lipoproteins .
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PMC9926039
P < 0.05 (P < 0.05) was considered statistically significant.
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PMC9119314
However, when compared with its cytotoxicity to Hep G2 cells, 6 exhibits better selective toxicity against gametocytes than 2 (SI = 13 and 2.5, respectively, Table 1).
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PMC6379402
Note that none of our data networks are well fitted by any of the tested random models (all MWU p-values were lower than 5%, as the two distributions do not overlap, e.g., Fig. 2d in the main document).
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PMC9429973
Six (7%) pts, 3 MPLS505N, 2 JAK2V617F, and one wild type, without thrombophilic abnormalities, experienced a thrombotic event.
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PMC11640555
Western blot analysis was performed to investigate CK2 levels in hCMEC/D3 cell lysates after treatment with CX-4945 at concentrations of 5 μM, 10 μM, and 15 μM. The results demonstrated that CX-4945 significantly reduced the p-CK2 levels at higher concentrations of 10 μM and 15 μM compared to untreated hCMEC/D3 cells (...
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PMC11395280
The cells were induced to migrate for 3 h at 37 °C in a 5% CO2 humidified incubator.
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PMC10237474
Therefore, our study strongly suggests that the growth advantages of Expi293F GnTI coupled with the robustness and simplicity of the PB transposon system makes Expi293F GnTI stable pools superior for producing high-quality soluble glycosylated proteins.
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PMC11774112
The iScript cDNA synthesis kit and SYBR master mix were purchased from Bio-Rad (Hercules, CA, USA).
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PMC9386809
However, further experimentation work is needed to establish the precise mechanism responsible for the anticancer activity of the synthesized complex.
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PMC11422497
RT-qPCR, reverse transcription- quantitative PCR.
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PMC9429973
All pts provided informed consent.
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PMC11593713
In the presented study OSI values were significantly increased in A549 cells after viral infections in all studied groups when compared to the control but not in MCR-5 cell lines.
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PMC11411004
ns, the difference was not significant. **
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PMC5941560
This fundamental TCR signaling defect in Jurkat led many researchers to question its validity as a model system for T-cell studies .
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PMC11394227
After that, the cells were analyzed by flow cytometry (Beckman Coulter, Brea, CA, USA).
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PMC11733919
Samples were prepared at a concentration of 0.02 mg of Fe/mL in Milli-Q water and sonicated 10 s before measurement.
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PMC11200978
In the case of LC, while numerous studies have demonstrated that CA can be used to effectively target NSCLC and support lethality in the −20 °C to −35 °C range , the definition of the MLT for LC remains unclear.
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PMC5035325
A p value, 0.05 was considered statistically significant.
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PMC10495912
Also, the Kollman bar was used to attribute the bar to the protein.
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PMC4270159
Each cohort will consist of cells treated with media alone, with vehicle alone, with the primary kinase inhibitor, with primary kinase inhibitor and the rescuing ligand and with the primary kinase inhibitor, the rescuing ligand and the secondary kinase inhibitor.
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PMC11021472
Mean ± SD of two independent experiments is shown. (
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PMC11746948
To elucidate whether 2-BP regulates ferroptosis at the transcriptional level, we performed RNA sequencing analysis on A375 cells with 2-BP treatment or not.
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PMC11700340
The study has found that linagliptin successfully reduces the growth of cervical cancer cells with a time-dependent cytotoxic pattern.
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PMC10882807
In this study, hsa_circ_0005397 was found to be significantly upregulated in HCC, and the good diagnostic sensitivity and specificity shown a potential diagnostic capability.
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PMC11762280
Similar results were observed in histological features; with increasing irradiation dose, the epidermal gradually thickened, the degree of epidermal necrosis and inflammatory cell infiltration gradually intensified, and the TUNEL positive staining in epidermis gradually increased (Fig. 1D).
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PMC11604768
Each TRE unit was examined for compatibility with our restriction enzyme strategy and modified as necessary to remove unwanted recognition sites.
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PMC9429973
The estimated median OS by treatment group (Figure 1C) was 8.4 mo with intensive CIT + ven, 10.6 mo with BTKi + ven +/- CD20 antibody and not reached with R-CHOP + ven.
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PMC10291556
Extensive studies on unconventional platinum(II) complexes of general formula [Pt(PL)(AL)], where PL is a polyaromatic ligand, and AL is an ancillary ligand, have yielded some promising results.
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PMC11534377
Pathogen-free eight-week-old female immunodeficient athymic nude mice (n=10) purchased from (Charles River, Hungary) were included in the study.
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PMC11806106
The opioid receptor family represents important drug targets, particularly in the management of severe pain which is mainly treated through the stimulation of µ opioid receptors.
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PMC11525028
Scale bars: 50 μm (left), 25 μm (right) (n = 5 per group).
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PMC11683240
Effect of the serpin proteins α1AT and antithrombin on intoxication of CHO-K1 cells with PT.A–C, PT (10 ng/ml) and α1AT (100 μM), antithrombin (AT) (100 μM), and fondaparinux (Fon) (1 g/l) or the respective amount of solvent (H2O) were added directly to CHO-K1 cells in FCS-free medium and incubated for 4 h at 37 °C.
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PMC9621239
In fact, patients with genotype C had a relatively poorer response to IFN than those with genotype B. Moreover, previous studies show that patients with genotype C harboured more rtA181T/V mutations than those with genotype B after antiviral treatment, and rtA181T/V was reported to reduce HBV susceptibility to LMV.
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PMC9429973
We found an increased risk of CHF for AML-LTS who have had AML relapse (OR, 3.16; 95% CI: 1.46 – 6.83; P=0.004) and, in trend, for patients with sAML or tAML (OR 2.19; 95%CI: 0.92 – 5.22, P=0.076).
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PMC9429973
The median OS and PFS time were not reached for the total population.
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PMC9878278
Therefore, we next assessed the gene expression of two key BMPs (BMP2 and BMP6) at different time points after infection.
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PMC11711063
The upregulation of laminins, fibrillins, and collagen IV indicated a change toward matrix deposition and increased density.
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PMC10820104
Consequently, there is an immense need to find new drugs or drug leads for curing this disease .
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PMC11593713
Based on the available literature, it was determined that each of the tested viruses replicated efficiently in the chosen cell lines .
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PMC11770199
The sizes of isolated tumor mass in the mMSCs-p14 group were significantly smaller compared to the saline group and the mMSCs-GFP group (Fig. 4G and H).
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PMC7553912
The apparent molecular weights are indicated on the right. (
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PMC5925824
Human complement was added into the assay plates and incubated for 1 h at 37 °C.
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PMC11541409
42(3_suppl): p. 748-748.This reference is of outstanding importance because it reports that ripretinib has the similar effcicay with sunitinib as a second line therapy drug for GIST patients after imatinib failure.
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PMC10538569
To obtain confocal images of GPI-GFP transfer to iDCs, 5×10 iDCs were resuspended in 300 µl of complete DMEM and seeded in 8 well chamber slides (IBIDI) 24 hours before imaging.
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PMC10142392
Click" reactions such as alkyne-azide and esterification have been particularly popular in this approach.
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PMC11730000
Specifically, it was noted that a concentration of 200 µM EGCG increased oxidative DNA damage in human lymphocyte DNA induced by H2O2.
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PMC11285179
Our work might provide a prospective molecular marker for guiding the clinical arrangement of combination therapy between anti-angiogenesis treatment and other therapies.
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PMC11772585
The reagents were added sequentially according to the manufacturer’s instructions, and a microplate reader was used to measure the glucose content in the culture supernatant.
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PMC9429973
Heterochromatin protein-1 alpha (HP1-α) IF (Immunofluorescence) staining was used to show the distribution of heterochromatin.
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PMC11155445
Sashankh et al. synthesized thirteen novel acyl thiourea derivatives via reaction of aliphatic/aromatic isothiocyanates and 4-(4-phenylpiperazin-1-yl)aniline in dry acetone.
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PMC10387886
Our results indicating the induction of EMT in SKOV3 cells upon TGFβ1 addition are consistent with the study carried out by Hou et al. .
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PMC11464982
This was done for all individuals across all surveys, resulting in distributions of individual average exposure.
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PMC10965680
Many of the genes found through signal analysis, such as CHRM2, SDC1, ITCH, and FGF12, are related to hair follicle growth.
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PMC9444294
After washing off the unbound primary antibody the next day, cells were incubated with PLA probes at 37°C for 1 h. Subsequently, they were incubated with ligation reagent for 1 h, followed by the amplification reagent for 100 min.
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PMC7685376
Then, liquid chromatography and tandem mass spectrometry (LC-MS/MS) analysis was performed according to the manufacturer’s protocol.
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PMC9503541
Molecular networking works by either matching experimental spectra against reference spectra (targeted metabolomics) or by aligning experimental spectra against one another and connecting related molecules based on spectral similarity (untargeted metabolomics) to find candidate metabolites directly from fractionated bi...
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PMC11489351
Gene names and GO terms were identified using the Ensembl version of BioMart (100).
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PMC6835428
Only a reduced amount of siRNA can be internalized via endocytosis.
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PMC9429973
Aims: 1.
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PMC11768927
Conversely, silencing HIF-1α expression using RNA interference significantly improved tumor susceptibility to VSV-mediated oncolysis.
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PMC11225860
During G1, red fluorescent cdt1-mKO is expressed which transitions to green as Geminin-clover is expressed during S-G2.
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PMC9874064
The green fluorescence comes from the cytoskeleton stained with Alexa-488.
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PMC11248911
Prior to production simulation, energy minimization of the system was carried out by using steepest descent algorithm (5000 steps).
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PMC11155445
The docking study also supported the above results and it was found that the compounds (151c and 151g) showed strong binding of −32.18 and −30.09 kJ mol, respectively (Fig. 78).
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PMC11767817
The most potent effect was observed for compound 2a at concentrations of 8 µM and 10 µM after 20 h of incubation.
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PMC9429973
The role of common functional innate immunology genes in the incidence in patients with AML remains unclear as previous studies provide conflict results.
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PMC11001582
Medium was exchanged daily for a total of 7 d to facilitate the removal of iPSNs that failed to recover postnucleofection.
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PMC6684588
Stop solution was used to halt the colorimetric reaction, which was read at a wavelength of 450 nm using a VersaMax microplate reader (Molecular Devices, San Jose, CA).
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PMC9065483
This previously unappreciated mechanistic insight adds to our understanding of key signaling pathways that contribute to successful OV therapy regimens and will facilitate pre-clinical and clinical development of vanadium compounds as enhancers of OV therapy.
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PMC11476837
Additionally, the expression of NRF2-regulated antioxidant and detoxification genes including SOD2, HMOX2, and GSS was maintained upon ML-385 treatment.
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PMC11092418
In addition, whereas treatment with all agents resulted in upregulation of the cells in the S phase of cell cycle in A172, the same treatments reduced the percentage of cells in the S phase in U118MG cells, highlighting the heterogenous nature of brain cancer cells.
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PMC11359735
We performed metabolic labeling experiments to assess the incorporation of radiolabeled GPI precursors into CSP.
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PMC11706776
RNA-seq was performed for ES and osteosarcoma cells and gene-level quantification was realized as previously described .
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PMC11711935
Furthermore, it can suppress Peroxisome Activator Receptor (PAR)‐2 expression in keratinocytes thus reducing melanin transfer.
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PMC11310713
At 12 weeks after HRT, uterine tissues were harvested from rats, and gross images were obtained.
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PMC11655498
XBP-1 can activate Rp independently, whereas the activation of Zp by XBP-1 requires cooperation with PKD (Bhende et al., 2007).
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PMC8557138
Emerging evidences have surfaced the role of the nervous system in regulating tumor angiogenesis (Jiang et al. 2020).
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PMC11564322
In the group co-treated with the VGVAPG peptide and the c-SRC inhibitor I, a 43.86% increase in the PPARγ protein expression was observed, compared to the control (Fig. 8E).
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