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OTAR3088
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BioData-CellLine_annotated

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PMC11802929
Notably, our analysis reveals that the anchor and exposed residues of the antigen exert different influences on T cell phenotype.
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PMC9429973
In pre-clinical models, these peptides can generate strong immune responses and specifically stimulate cross-reactive cytotoxic CD8 T cells to recognize the chosen B cell targets.
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PMC11680562
Initially, 1 gm of 0.1% (w/w) PXFCu gel (1 mg/gm of 5-Fu) was placed on the dialysis membrane bag and immersed in a beaker with 100 mL of acetate buffer solution (pH 4.2) at 37 ± 1 °C, stirring consistently at 100 rpm.
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PMC10654497
We then performed functional enrichment analyses on these DEGs using the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases.
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PMC11679033
Final concentrations from 0.05 to 30 μM were prepared in a growth medium without supplementation.
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PMC11514418
Among them, 41 patients received APS combined with GS chemotherapy, and 56 patients received GS chemotherapy only.
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PMC11705862
Treatment of the SH-SY5Y cells with BDNF and ATRA differentiates the cells into mature, neuronal phenotype .
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PMC4270159
These experiments were performed in 8505C cells, whereas M14 cells were used in the study being replicated.
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PMC8540692
The nasal PAMPA plate was incubated for 1 h, while PAMPA BBB was incubated for 4 h in a humidity-saturated atmosphere at the temperature set at 37 °C.
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PMC9429973
The real time PCR showed that the mRNA expression of CD47 in U937 and HL-60 cells of hypoxia group were upregulated (P<0.05).
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PMC10926885
Figure 8D).
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PMC11786767
After gelation was completed, the hydrogel was immersed in trypsin digestion solution (0.05% volume dilution: 2 μl trypsin-EDTA solution in 4 ml low salt digestion buffer), and then incubated at room temperature for at least 20 h with gentle shaking.
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PMC11365427
Primary antibodies used were as follows: anti-β-actin (Cell Signaling Technology, 4967, 1:3000), anti-p-PERK (Proteintech, 29546-1-AP, 1:1000), anti-PERK (Proteintech, 24390-1-AP, 1:1000), anti-p-eIF2α (Proteintech, 28740-1-AP, 1:1000), anti-eIF2α (Proteintech, 11170-1-AP, 1:1000), anti-ATF4 (Proteintech, 10835-1-AP, 1...
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PMC9429973
However, immune dysfunction in cancer is multifactorial requiring multiple points of action, especially in immunologically-cold tumors Aims: The aim of TEM-GBM study is to evaluate the safety and the efficacy of Temferon in up to 21 newly diagnosed patients with glioblastoma and unmethylated MGMT promoter Methods: We h...
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PMC9739791
The toxicity of the Pt(IV) prodrug 27 was low in the absence of irradiation and a 10–25-fold increase was observed after irradiation with white light (400–700 nm, 13 mW/cm, 30 min) (Table 8).
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PMC8389560
Total RNA from A-172 cells after 24 h of treatment with various concentrations of SeNP was isolated using ExtractRNA reagent according to the manufacturer’s instructions (Evrogene, Russia).
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PMC11541409
Approved TKIs for GIST treatment are categorized into two groups: type I and type II kinase inhibitors.
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PMC10994876
Phosphorylation of YAP, recognized as a central element in the Hippo pathway, induces its confinement within the cytoplasm and subsequent breakdown through its interaction with 14–3-3 proteins and b-TrCP, respectively.
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PMC11281366
CD4 T cells were isolated from PBMCs via negative depletion using the human CD4 Isolation Kit II (Miltenyi, San Diego, CA, USA, #130-096-533) according to manufacturer’s instructions.
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PMC11607638
KillerRed: λ ex, 561 nm, λ em 618 nm; DAPI—λ ex 405 nm, λ em 477 nm.
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PMC11772585
After 48 h of transfection, the cells were transferred to 96-well plates and the luciferase activity was measured with the dual-luciferase reporter assay system, following manufacturer’s instructions.
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PMC9429973
Ecuador is a low-income country so access to Tocilizumab was delayed, that is why the goverment approved the use of convalescent plasma on april 2020 as an alternative of therapy specially with critically ill patients.
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PMC10847511
The HRD score and signature 3 were positively correlated in the CCLE dataset (n = 914, Spearman r = 0.475, p = 1.25 × 10, Fig. 2a).
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PMC11337594
A375 control and p53-KO cell lines were treated with different doses (0, 1.0, 2.0 and 3.0 µg/ml) of TER, and the image shows the expression of cell cycle-related proteins (Cyclin A2, Cyclin D1, Cyclin E1, CDK2, and p21). (
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PMC10218459
The local treatment of ES involves surgical excision in combination with preoperative or postoperative radiotherapy to eliminate possible tumor residues at the margin of resection .
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PMC9596868
Ekta Khattar: Data curation, Writing – review & editing, Funding acquisition.
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PMC7917457
A549 cells were infected with VALO-D102 at a MOI of 10.
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PMC11469524
Regarding the perturbation coefficients we distinguish between stimulation and inhibition.
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PMC9844987
Although the shape and distinct biological mechanisms of these promoter classes, for example, housekeeping activities of broad promoters, are conserved across species (Carninci et al., 2006; Hoskins et al., 2011), the necessity for positional dispersion of TSSs and its association with promoter variability are poorly u...
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PMC11730311
Vessels were then allowed to equilibrate for approximately 30 min before being exposed twice to a bicarbonate buffer containing 60 mM K to induce contraction of arteries, thus confirming their viability.
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PMC9429973
This study confirms recent findings suggesting a prognostic role of this mutation type.
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PMC11751675
They serve as key precursors for the generation of various SLs with different polycyclic skeletons, such as guaianolides, eudesmanolides, and other sesquiterpenenoids.
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PMC9919300
Notably, the treatment with curcumin and andrographolide reduced the number and the size of LDs in the steatosis model alone and in association (Figure 3a).
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PMC8526701
Left, representative TUNEL staining pictures; Right, TUNEL-positive cells were counted and plotted, Scale bar: 25 μm.
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PMC10048572
Here we report the association of increased aberrational load with the slowly growing subclones.
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PMC9429973
By univariate analysis, morphologic subtype, AML genetic classification, fibrinogen, d-dimer, and DIC score were not associated with thrombosis during induction in AML.
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PMC11612315
Detection of the interaction between exendin 9–39 and TRPV1 via western blotting with an anti-human TRPV1 antibody.
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PMC11367146
The binding energy of the BCL2-gigantol complex was −9.5 kcal/mol, with hydrogen bonds forming interactions with ASP 107, LEU 108, SER 106, GLU 129, and SER 145 amino acid residues.
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PMC10419319
Isoalantolactone treatment induced increased phosphorylation of p38 MAPK and ERK1/2, whereas cisplatin treatment decreased levels of these proteins in SNU-8 cells.
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PMC11271278
We found that the same oncogene hijacks different enhancers from chromosome 8 in the breast cancer cell line ZR751 (Fig. 3C).
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PMC11612626
Treatment of RPMI-8226 cells with compound A increased HLA-B presentation by ∼2-fold (P = 0.01), the HLA-A × 30 subtype by 1.5-fold (P = 0.0001), and the HLA-B15 subtype by 1.8-fold (P = 0.01).
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PMC11066331
In summary, the intact cell MALDI-TOF MS combined with proper biostatistical tools can offer a versatile tool for quality control in preclinical or clinical-grade cell cultures, cancer cells discrimination, or gamete phenotyping .
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PMC9926039
The cells were incubated in a humidified atmosphere containing 5% CO2 (at 37°C), and harvested at 70%–80% confluency with a culture medium renewal every 3 days.
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PMC11194678
Upper: Representative Western blotting images of mTOR, p-mTOR, p62, and LC3B-II levels in SH-SY5Y cells expressing PEGFP-C3, GFP-STAU1 WT, or 5KE.
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PMC9429973
Further analysis will unravel the impact of high FGF23 levels on other BM niche populations involved in the crosstalk with HSC.
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PMC11394112
Cells were harvested, washed with cold PBS, and lysed on ice with RIPA buffer (Boston BioProducts, Milford, MA, USA) and 1× HALT protease/phosphatase inhibitor (ThermoFisher, Waltham, MA, USA) for 30 min.
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PMC8984067
Regarding IR spectrum for UCNPs, the peaks occurring at 3427, 2957, 1470, and 1314 cm correspond to N-H stretching vibrations, C-H stretching/bending vibrations, and C-N stretching vibrations, respectively, which all originated from PEI, indicating that PEI successfully modified NaYF4:20%Yb, 2%Er nanoparticle surfaces,...
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PMC9429973
Baseline characteristics of prognostic significance (such as cytogenetic profile, International Staging System stage, presence of extramedullary disease, number of prior lines of therapy, and refractory status) were adjusted for the analysis.
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PMC11742290
Two recent ovarian cancer studies also reported that high LGR5 mRNA expression was significantly associated with increased progression-free survival (PFS) in high grade serous ovarian carcinoma .
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PMC11794568
The overall survival (A), disease-specific survival (B) and progression-free interval (C) of melanoma patients with low CRIP1 expression were shorter than those with high CRIP1 expression.
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PMC11066331
Josef Havel: Writing – review & editing, Validation, Funding acquisition, Formal analysis, Conceptualization.
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PMC11064533
Upon adhesion to the 5637 cell line, ΔflgH did not develop a clumpy phenotype and had lost the ability to adhere.
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PMC11732635
In situ hybridization showed positivity for EBV-encoded small RNA (EBER), and the lymphocyte subtypes of EBV infection were determined by fluorescence quantitative polymerase chain reaction at Shanghai Children’s Medical Center (SCMC) (Figure 1).
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PMC11052306
10 μM .
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PMC5716223
d Representative western blot of αB-crystallin and Hsp27 expression.
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PMC8567602
As the hSS2 allele in our mouse model resides at the Rosa26 locus, both Ss18 loci are retained in their native state—unperturbed by chromosomal translocation—likely contributing more SS18 to compete with the fusion for occupancy in BAF-family complexes.
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PMC11139449
Based on HITI, the ORANGE platform facilitates precise tagging of neuronal proteins at nanoscale resolution ( 24 ).
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PMC7602170
According to this study, RAD001 has effects on suppressing cell proliferation .
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PMC8143558
Third, using soapy plants may reduce the amount of water that is required for disinfection since aqueous extractions of saponins may not require large amounts of water.
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PMC10759659
However, it has several limitations in clinical practice.
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PMC10048572
Not all the newly acquired mutations may relate to the emergence of resistance or metastasis.
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PMC9429973
Methods: Association of molecular profiles with responses to RVU120 has been performed on genetically annotated AML PDCs, followed by flow cytometry and bioinformatic analysis.
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PMC11684269
Conversely, deficiency of FASN in Treg cells alleviates tumor growth (49).
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PMC11490153
The percentages for each group are as follows: control: 7 ± 2%, AlgNPs: 9 ± 2%, C. citratus EO: 10 ± 4%, citral: 15 ± 3%, C. citratus-AlgNPs: 20 ± 3%, and citral-AlgNPs: 16 ± 2% Flow cytometry results of the A-375 cell line treated with samples under normoxic conditions, showing the percentage of apoptotic cells (early...
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PMC9429973
It consists in an immune response of the mother against non-self antigens expressed by the fetus, which derive from the male partner, and its manifestation is an hemolytic disease of the fetus and newborn (HDFN).
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PMC11641630
Three hours later, the cells were washed five times with warm PBS and detached with 0.2 mL of trypsin (Biowest, Nuaillé, France).
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PMC9429973
Despite this, there is no data about this alteration from MM patients in Latin America (LA).
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PMC11678368
Such a band is not observed for the cis conformer of the 4ClL ligand , nor for its complex trans-[PdCl2(4ClL)2] (this work).
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PMC4360730
Transitive relationships remain true across multiple links of the relationships.
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PMC7039683
PWM, position weighted matrix.
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PMC4355729
To determine the function of AKT in the mutated cells, depletion of AKT were performed in 786-mut1 and A-704-mut2 cells.
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PMC11718031
Figure 2D shows a heatmap illustrating the amino acid distribution in all media tested.
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PMC11754094
Another complicated step in the workflow is sequence alignment of short-read NGS data.
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PMC8345486
Only a slight but significant increase in pSer15-p53 expression was observed under the influence of compound 2 in A2780 cells (p < 0.05, Figure 8).
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PMC11357960
In this study, we focused attention on the mRNA expression of 15 SUMO machinery components, including four SUMO genes (SUMO1~4), two SUMO activating enzyme genes (SAE1 and UBA2), a SUMO conjugating enzyme gene (UBE2I), two SUMO ligase genes (PIAS1 and PIAS2) and six SUMO specific protease genes (SENP 1,2,3,5,6,7), in p...
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PMC8873393
The experimental strategy described in this study is critical in its ability to simultaneously provide information about the action of transcripts (both mRNA and noncoding RNAs) that remain associated with chromatin and the changes the transcripts proceed to the cytoplasm.
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PMC9429973
Female SCD patients and patients with HbSS/HbSβ genotype were at significant lower risk of developing SCR than other patients.
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PMC10092581
Despite the considerable number of tested complexes, the investigation of their biological and chemico‐physical properties did not allow the elucidation of structure‐activity relationships.
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PMC11775197
Subsequently, this results in an increase in glycolysis and the regulation of enterocyte differentiation .
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PMC11411004
a I-4 molecular structure formula.
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PMC11752767
A clinical study of TIL therapy over a 62-month follow-up period reported a 56% (52 in 93) objective response rate, among whom 20 patients underwent complete remission.
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PMC11767817
Finally, 100 µL of Assay Buffer was added to all wells, and fluorescence was measured using a Tecan plate reader (Infinite M Plex, Männedorf, Switzerland).
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PMC11754094
However, we also observed that the frequency of large deletions was decreased in the RAD52 KO cell line, opposite to Cas9 nucleases (Fig. 5e), possibly implying a difference in DNA repair processes between Cas9- and PE-driven DSBs.
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PMC11575040
In summary, we identified two miR-1270 target genes, CD276 and CENPM, which may contribute to metformin-induced growth suppression via modulation of the LINC00094/miR-1270 axis in melanoma.
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PMC11650848
By altering DNA methylation levels, we demonstrate that this in turn controls the expression of the ZFP57 isoforms.
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PMC11064533
Sequences of all the deletion mutants for this study are available in the NCBI BioProject database under accession number PRJNA880721.
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PMC4270159
Serum starve = DMEM + 0% FBS.ii.
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PMC10734950
This study observed hypophosphorylation in JAK 1 and JAK 2, suggesting that F8 inhibits this pathway reducing proliferation and angiogenesis and promoting apoptosis.
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PMC7823217
Figure A is reproduced from with the permission of Springer Nature.
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PMC11200978
The combination of gemcitabine pretreatment and a −20 °C single freeze as well as combination treatment with a −15 °C repeat freeze both resulted in complete cell death.
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PMC10820104
For analysis of the cell cycles, 9 × 10 cells/mL of PANC-1 were cultured in 6-well plates (Greiner Bio-One).
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PMC11721064
A Representative images of KPCs cultured in KSFM, LN-KS medium, and LN-SH medium.
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PMC11594074
Molecular dynamics (MD) simulations were performed to evaluate the stability and flexibility profiles of the compounds in the DNMT1 binding site.
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PMC11453018
In contrast, the viability of the non-sarcoma human myoblast LHCN cell line and the osteosarcoma cell line remained unaffected at this concentration (Fig. 2A and Supplementary Fig. 1A).
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PMC9429973
Belamaf PK profile was similar to that in pts with RRMM, accounting for baseline characteristics.
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PMC11451940
ICP-MS analyses were performed using an Agilent 7700x inductively-coupled plasma mass spectrometer (ICP)-MS (Agilent, Santa Clara, CA, USA).
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PMC8701144
The adenoviral E1B-55k is a multifunctional protein that interacts with numerous viral and cellular factors .
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PMC9429973
Every clone was analyzed as an event, comparing its DNA ratio with its cDNA ratio.
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PMC9429973
Part A explored the continuous daily dosing of IOA-244 at 10, 20, 40 and 80 mg in pts with solid malignancies and at 20 mg and 80 mg in pts with FL.
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PMC11644761
A growing body of research is exploring the role of these compounds in reversing or delaying the onset of age-related diseases .
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