PMCID
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PMC5308810
After continuous culturing for 21 days, their morphology became similar, presumably because OVC prefers to maintain an equilibrium state between the two cell populations. (
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PMC11682525
Additionally, these stem cells have shown the capacity to secrete various neurotrophic factors ( 32 , 33 ), further enhancing their therapeutic potential.
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PMC9454178
Consistent with this, we validated with tissue microarrays (TMAs) that CDK7 overexpression in GISTs was correlated with tumour progression and an unfavourable prognosis.
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PMC11001582
f, Representative EDL MUNE traces from 12-month-old WT (left panel), (GR)400 (center panel) and (PR)400 (right panel) mice.
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PMC11706776
As expected, DiBAC4(3) fluorescence measurements showed the ability of compound GW542573X, an activator of the SK1 channel to hyperpolarize the cell membrane (Fig. 5C).Fig.
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PMC7452526
Caco-2 cells were treated with CN–Ag at different concentrations 5, 10 and 30 μgmL and evaluated for cell viability after various exposure times 24, 48 and 72 hrs (see Figure 5).Figure 5MTT assay for cytotoxicity investigation of colloidal nanosilver against Caco-2 cells at different concentrations 5, 10 and 30 μgmL an...
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PMC9318744
USA-WA1/2020 strain (BEI#NR-52281) was propagated by infecting Vero E6 cells at a low multiplicity of infection (MOI) and then maintaining the infected cells in Eagle’s Minimal Essential Medium (EMEM, Millipore Sigma, Burlington, MA, USA) with 2% fetal bovine serum (FBS, Atlas Biologicals, Fort Collins, CO, USA).
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PMC9509578
Mammalian P-gp is the most widely studied transporter and it plays a significant role in MDR .
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PMC4355729
The number of tumor spheres was counted 14 days after seeding.
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PMC11697703
Corticospinal projection neurons and interhemispheric callosal projection neurons are among the best-characterized neuron subtypes representing the corticofugal and corticocortical broad neuron classes.
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PMC9587866
The active site residues predicted by Prank-Web (Jendele et al., 2019) was specified to allow site specific interaction of ligands.
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PMC3019555
The number of Ago2 foci per cell was quantified (100 cells/condition) and plotted as percentage of cells with either three or less or more than three P-bodies (mean ± SEM; n = 3; **, P < 0.01). (
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PMC11605551
Thus, elucidating the mechanisms of invasion and leptomeningeal metastasis is important to improve the prognosis of malignant brain tumors.
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PMC10926885
Consensus normalised expression (NX) levels of NDE1 in dozens of normal tissue types and blood cell types generated from three transcriptome datasets (GTEx, HPA and FANTOM5); (B) Pan‐cancer differential expression of NDE1 in paired tumour and adjacent normal tissues in indicated tumour types from TCGA database; (C) NDE...
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PMC4360730
Treatments that can be applied to a given biosample may be chemical or biological.
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PMC10547921
Chemical structures of tubulysin analogs 28–34; (B) Binding mode of compound 30 in complex with tubulin (PDB code 6Y4N).
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PMC11365427
Mouse weight was recorded and showed weight loss after phenylalanine deprivation (Fig. 8E).
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PMC11502327
Studies have shown that Fisetin can play an anti-OC role in various ways such as anti-inflammatory, anti-proliferation and apoptosis-inducing (213).
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PMC11089031
E the KD of ZNF692 inhibited the migration of Caki-1 cells.
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PMC10968586
The ingestion of 40 mL of high (366 mg/kg)-phenolic-compound-content olive oil led to a plasma HT concentration peak of ≈15 μM (mean time to peak ± S.D. = 0.91 ± 0.84 h, mean estimated elimination half-life ± S.D. = 3.00 ± 1.46 h), whereas the same amount of low- (2.7 mg/kg) and medium (164 mg/kg)-phenolic-compound-con...
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PMC11594074
In silico methods might not necessarily reflect the full reality, but they are valuable in offering initial insights into molecular interactions.
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PMC9985266
DNMT1 is associated with the development of PF.
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PMC11629461
CHO‐K1 cells were transfected in this work with plasmid pcDNA /NOX5.
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PMC10887351
NB patients present high levels of CA that reflect the severity of the malignancy .
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PMC11394112
In NSG-IL6 mice, systemic engraftments of both U2932 activated B cell-like- and VAL germinal B cell-like-DLBCL (engraftment rate; 75% and 82%, respectively) were detected within 2nd-week post-injection.
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PMC11409031
To illustrate the similarity in gene expression, Pearson correlation coefficients were utilized to generate a topological overlap matrix (TOM).
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PMC8097906
The two most common subtypes are the embryonal (RME; 70%) and the alveolar (RMA; 20%) rhabdomyosarcomas .
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PMC11143482
Approximately 68% of patients with localised OS live for 5 years or more, but regrettably, 20–30% of these patients have metastatic or recurrent malignancy and lung metastasis is the most prevalent type of metastatic dissemination .
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PMC7504302
High rates of gene fusions; splicing defects; and frameshift, missense, and nonsense mutations that result in low expression or absence of the 70 kDa tumor suppressor protein Merlin (moesin-ezrin-radixin-like protein), the protein encoded by the NF2 gene, have also been reported .
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PMC10530622
Concentrations above 300–500 µM might be reached at the cortical collecting duct and even higher concentrations can be found in PH or after bowel resection as reviewed by John Asplin in 2002, evidencing genetic and non-genetic factors underlying variable oxalate handling among individuals .
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PMC9429973
SCTC-DI was calculated as previously reported.
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PMC11757230
Data are expressed as the mean ± standard deviation (n = 6).
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PMC9429973
Median age was 8.5 y (3-19).
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PMC11656657
In line with this observation, it has been shown that several tumors expressing high levels of G-CSF are aggressive, more difficult to treat, and present with poor prognosis and high mortality rates .
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PMC11322866
P<0.001.
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PMC9024365
Neurons were treated with 200 μM glycine or vehicle in aCSF without magnesium at 37C for 5 min to induce chemical-LTP.
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PMC11762280
We hypothesized that, unlike atopic dermatitis, the effects of keratinocyte PANoptosis in acute UVB-induced skin injury may be limited to mediating keratinocyte death, yet interplay with immune cells may not be significant.
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PMC11599565
SKOV3 cells were exposed to cisplatin for 72 h to study the first biological response to this agent.
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PMC11794847
Finally, we also predicted GATA1 as a transcription factor of HVEM.
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PMC11387401
This study has some limitations.
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PMC11322866
EZH2 is a histone methyltransferase involved in the development of MM by trimethylating histone H3 at Lys27, thereby silencing target genes.
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PMC4659567
The complexes 1–8 were obtained in the yields of ca 60–80% (related to the starting Ru(II) dimer) and their composition and chemical purity were proved by a combination of elemental analysis, H NMR spectroscopy in CDCl3 (Fig 2) and a single-crystal X-ray structural analysis.
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PMC10551595
AO/EB staining and flow cytometry showed the apoptotic nature of the CCRF‐CEM cells followed by their treatment with GinA. Results indicated that 10 μM GinA may increase apoptosis in CCRF‐CEM cells by 18.37‐fold (mean ± SEM, p < 0.0001), but not necrosis (p = ns).
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PMC11703992
The result confirmed the causal role of DDIT4 and ARHGAP15 to GCA risk.
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PMC11269792
CircAPP activates GIST proliferation and the Warburg effect by regulating the miR-6838-5p/CDV3 axis.
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PMC7987994
Sole extraction medium served as a control (unaffected cells which metabolic activity was taken as 100%).
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PMC11180402
For cDNA synthesis, 5 mg of RNA was processed with oligo(dT) primers utilizing the SuperScript First-Strand Synthesis System (Invitrogen).
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PMC7642379
Along with changes in extracellular matrix genes, there is also a consensus amongst progeny cell lines compared to HEK293 of differential expression of genes involved in other types of cellular component organization such as cell morphogenesis, cytoskeleton-, membrane- and cell junction organization.
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PMC10652261
Mp 145–146 °C.
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PMC11171319
Among the viral proteins, the immediate early protein 1 (IE1) favors stemness and EMT in glioblastoma cells .
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PMC11577421
CHO-K1 and pgsA-745 cells were incubated with NPs (3 nM) for 4 h in culture medium, washed to remove excess particles, then analyzed by flow cytometry.
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PMC11597291
To further investigate the anticancer ability of nCGO-PEG/PCT particles in relation to free PCT, the apoptotic effect against A549 cells was examined (Figure 5F).
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PMC8170755
VPA increased and reduced the expression of Nm23H1 and CD44v6 protein in A549 after 72 h, respectively Primer sequences were used in RT-PCR
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PMC11566417
n = 3, p < 0.05.
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PMC10728535
Table 2 shows brief information of cell line samples used in this meta‐analysis.
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PMC11474209
Tumorigenesis36HL−77025 or 10 µMHSP27, F-actin, α-tubulinPP2A, MAPKs, ERK1/2, phosphorylated JNK and phosphorylated p38 MAPKMicrotubule and actin filament reorganization, cytoskeleton modifications, and minor changes in cellular morphologyReorganization of the cytoskeletal structure37The human amniotic epithelial cells...
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PMC8708099
The cell culture plates were centrifuged at 4 °C, 800× g for 10 min, and post-culture media were collected in tubes and stored immediately at −80 °C for further analysis.
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PMC11401358
AFM characterization of SF-95 °C, revealing dense nanofibres. (
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PMC11264242
Therefore, at least in part, by blocking FYN activity, PP2 synergizes with FK228 to reduce synovial sarcoma cell migration.
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PMC11172027
It was an immortalized line of human T-lymphocyte cells derived from acute T-cell leukemia.
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PMC10745221
These results suggest that protein arginine methylation may not be important in altering the osteosarcoma cells’ methionine addiction.
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PMC10820104
There is also an evidence that this fungus is able to synthesize prenyltransferases.
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PMC11759751
Furthermore, after co-culture with CD19 CAR T cells, CD19 CAR T cells were stimulated to secrete more cytokines (IL-2, TNF-α, and IFN-γ) (Fig. 4E).
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PMC10850553
Because the selective mTOR inhibitor, Torin1, could mimic the effect of the starvation on the expression of C5ar1 at the dose- and the duration-dependent manner (Fig. 3B) and also on the phosphorylation of the downstream factor of mTOR signaling, p70 S6 kinase (S6), in western blot analyses in PDGF-BB pre-treated cells...
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PMC11297139
Our findings revealed that, while the condensates detected in osteosarcoma patient tissue and the osteosarcoma cell line SAOS-2 appeared morphologically smaller and less defined compared to those in Ewing’s sarcoma, a distinct punctate pattern was clearly observed in the nuclei (Supplementary Fig. 4a, b).
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PMC11591052
While several studies have explored the anabolic activity of 20E in muscle cells, they have concentrated on its effects on myofibril size, protein biosynthesis intensity, and myostatin expression, without assessing energy metabolism.
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PMC9429973
Results: The percentage of NK cells among lymphocytes was comparable in patients and HC.
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PMC11342785
C, schematic overview and quantification of light-induced cluster formation for different TPPP variants in cells.
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PMC9621239
To obtain HCC cell lines stably expressing wild-type and mutated HBx, Huh 7 and Bel-7404 cells were infected with the Lv-HBx-WT, Lv-HBx-L123S, Lv-HBx-S101P/L123S, Lv-HBx-T81P/S101P/L123S and lentivirus empty vector (GenePharma, China).
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PMC11696659
The samples from healthy subjects that were used as controls were collected at the Centre for blood transfusion of the Autonomous Community of Madrid (Madrid, Spain) from subjects aged >18 and < 65 years, and at the Hospital Príncipe de Asturias Hospital (Alcalá de Henares, Spain) from subjects aged >65 years.
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PMC6267596
This set up can image live cells for 6-day periods without a reduction in the growth rate or viability of cells .
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PMC11395280
To examine the effect of CCL198-83 on survival, we used the well-characterized PRDM14-inducible model.
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PMC9429973
Median ferritin level was 77 ng/mL, with more than 400 ng/mL in 10 patients.
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PMC9817179
We show the percentage change of LLPS parameters after 10 min of fixation.
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PMC11532793
The time spent by Salmon to draw bootstrap resamples represented 84% and 76% of the total quantification time for libraries with 25 and 100 million reads pairs, respectively.
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PMC11507371
Thus, the results of this work align with the current understanding of IKKß’s role in cancer biology.
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PMC2944824
The granule layer of the cerebellum is shown (B).
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PMC11335267
Since Cart mRNA expression is regulated by circulating leptin , we measured the levels of this hormone in the blood and found that they were not significantly different in Alx3-deficient and control mice (Supplementary Fig. 12A).
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PMC11574271
Tumor growth was monitored every 2 days by measuring individual tumor volumes using the formula: V = L × W2 × π/6 (V, volume; L, length; and W, width of tumor) as previously described .
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PMC10994876
In comparison, the OE-YAP5SA group showed a significantly lower mean percentage of apoptotic cells, measuring 3.83% ± 0.77% (mean ± SD).
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PMC9429973
Combination therapy was approved for clinical use by the European Medicines Agency in 2016; additionally, a recent British Society for Haematology guideline states that DFP+DFX is a highly effective combination therapy, although the safety and efficacy of DFX in combination with other iron chelators is not well establi...
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PMC10978090
HIT antibody testing was performed on citrated plasma samples using a commercial enzyme-immunoassay (EIA) specific for PF4/H IgG (Zymutest HIA IgG, Hyphen BioMed, France).
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PMC11774112
However, as this study was preliminary and conducted in melanoma cell lines, it has certain limitations.
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PMC9429973
Results: Aggregate data for 2,600 SCD patients in BHR, 2,000 in OMN and 350 in UAE were collected from hospital records data bases.
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PMC11703992
Additionally, the supernatant of cell culture medium was also collected for detection of secreted inflammatory factors.
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PMC11342785
Scale bar represents 10 μm. (
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PMC10743717
to mitochondria and then published in the Science journal as a new discovery in 2004 by omitting all previous literature already showing the intra-mitochondrial localization of the human , mouse and rat HSD17B10 (HADII) gene product—17β-HSD10 or SCHAD.
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PMC7171147
GAPDH was used as a loading control.
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PMC8170755
Western blotting The cells seeded in 6-well plates were scratched and transferred to a microtube.
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PMC11684297
Moreover, ThT has been known to bind with the side chain channels that run along the amyloid fibril’s long axis .
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PMC9429973
In acute myeloid leukemia (AML) patients, STAT3 has been reported to be constitutively activated thereby promoting proliferation and blast survival.
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PMC11615828
Afterward, cells were incubated with Alexa Fluor 647 goat anti‐human IgG (H+L) antibody (1:1000; Invitrogen) for 1 h at room temperature to label the SARS‐CoV‐2 RBD protein within the entire cells.
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PMC2196094
The screening of the large unamplified cDNA library showed that serosal MCs are highly specialized cells which most likely continuously produce large amounts of the various secretory granule proteases, and with mRNA frequencies for these proteases in the range of several percent of the total mRNA pool.
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PMC11754094
NS, not significant. *
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PMC9429973
Eight (16%) pts had additional chromosomal abnormalities (ACAs).
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PMC8164677
As far as the question of origin of EOC is concerned, the ovarian surface epithelium itself, its cysts and non-ovarian structures (including peritoneum, endometriotic lesions and fallopian tubes) have all been hypothesized as being its source, although knowledge of its etiology remains poor.
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PMC9429973
She had no bleeding complication during tooth extractions or vaccination.
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PMC11544771
As shown in Figure 5B,C, 72 h treatment of A549 cells with CPX (5 μM) or PRE (20 nM) alone increased the percentage of apoptotic cells by approximately 3.4-fold and 3.6-fold, respectively, and the combined treatment further increased the percentage of apoptosis cells by about 6-fold, compared with the control.
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PMC11641532
For knockdown experiments, siRNA targeting HSD3B7 was transfected into 769-P cells using Lipofectamine 3000 (Thermo Fisher Scientific, Cleveland, OH, USA) following the manufacturer’s protocol.
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PMC11794588
Fig. 10Mass spectra of Compound 2 (Cedryl Acetate) and its structure.
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