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PMC9429973
Results: As of Jan 15, 2021, the median (95% confidence interval [CI]) DOT was 11.70 (8.97–16.33) mo for luspatercept pts and 5.52 (5.52–5.59) mo for placebo pts.
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PMC9429973
The work was financially supported by project NU20-05-00282 of the Czech Republic Ministry of Health M. Kusterer, M. Lahnalampi, M. Voutilainen, G. Gentile, S. Pennisi, J. Norona, G. Greve, M. Lübbert, R. Sankowski, M. Prinz, S. Killmer, M. Salvat Lago, B. Bengsch, M. Cleary, V. Zachariadis, M. Enge, O. Lohi, M. Heinän...
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PMC7724160
3/4).
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PMC11711127
The interpretation of the kinapse mode of interaction must be considered in a functional context as kinapses can be part of a mitogenic/activatory programme, or anergy.
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PMC11534035
The juxtaposition of our results with those of Whitley et al. highlights the complexity and variability between different cancer entities and could be proof that PAI1 plays varying roles depending on protein expression and type of cancer.
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PMC10729469
To confirm Aβ specificity of the engineered Tregs, tetramers of Aβ1-42 T cell epitopes presented by H-2 haplotypes were constructed with I-A and the Aβ amino acid 15–30 (MHCII-IA–KLVFFAEDVGSNKGA) conjugated to fluorophore BV421 (National Institute of Health (NIH) Tetramer Core Facility, Emory University, Atlanta, GA).
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PMC11547972
However, others assume that SFN may impair the anticancer effect of ICI on gastric cancer cells .
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PMC9429973
Treatment was discontinued due to AEs in 5% and 21% of pts, respectively.
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PMC11441402
This assay is more difficult to perform with suspension or semi-adherent cells as viable cells can be lost during wash steps.
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PMC11787651
The five histone proteins (H1, H2A, H2B, H3, and H4) are extensively modified, with over 75 distinct modification types spanning more than 200 sites.
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PMC9429973
In contrast, several European consortia reported inferior real-world response rates and survival.
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PMC11200978
SytoxRed was then added to each sample and incubated for an additional 5 min at 37 °C.
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PMC8633974
To further survey payload delivery to the nucleus, parallel experiments were performed with fluorescently tagged nucleic acid.
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PMC11392912
Briefly, 200 µl plasma was mixed with 1 ml of QIAzol lysis reagent, incubated for 5 min at RT, and 200 µl of chloroform was added, followed by centrifugation at 12000 × g for 15 min.
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PMC11144382
Moreover, RA has been reported to significantly increase VCAM1 antigen expression in human neuroblastoma cells.
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PMC11779605
Data were analyzed using the ROSALIND online software (https://rosalind.bio/), with a HyperScale architecture developed by ROSALIND, Inc. (San Diego, CA).
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PMC10671321
He signed a written informed consent for his participation in a research program that was approved by a scientific ethical committee (authorization AC-2018-3156).
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PMC11496728
P < 0.05 and **P < 0.01 compared to the control group.
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PMC11597291
PEG chains probably generate a hindrance to the efficient approach of PCT on the nCGO surface, thus reducing drug loading capacity, and the longer the chains of PEG, the more pronounced this effect is.
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PMC10926885
A, B) The correlation between NDE1 expression and overall survival (OS) in various cancers in TCGA database; (C, D) The correlation between NDE1 expression and recurrence free survival (RFS) in various cancers in TCGA database.
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PMC11672881
Some agents may complement each other by increasing their effectiveness, thus inhibiting tumor growth, proliferation, and metastasis more effectively than a single agent alone.
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PMC11306331
E Schematic of approach for chemogenetic dissolution.
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PMC11682224
They were then incubated for 24 h at 37 ℃ with 5% CO2.
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PMC11024707
The tube, in which the blood was collected, was kept upright at room temperature for 30 minutes without mixing or touching.
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PMC9429973
Nor were clinically significant results obtained between the EMEA and US cohorts.
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PMC9429973
And we compared them to a control group with 485 NDMM patients.
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PMC11522251
4By inhibiting STAT3 signaling, HOXB8 knockdown increases the susceptibility of HCT116 and CACO2 cells to cetuximab (A-B) HCT116 and CACO2 cells with knockdown of HOXB8 were handled with varying dosages of cetuximab (0, 100 μg/ml).
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PMC11593713
Each virus was cultivated on its default cell line.
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PMC10890055
In vitro testing is the first important step in the development of new biomaterials.
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PMC11713679
The oxygen consumption rate, Total Reactive Oxygen Species (ROS), and 2',7'‐dichlorofluorescin diacetate (DCFDA) assays were conducted to evaluate cellular responses.
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PMC11021472
To conclude, our results show that circZDHHC11 is a crucial factor supporting BL cell growth independent of its ability to sponge miR-150.In our previous work, we have shown expression of a circular RNA (circZDHHC11) derived from the ZDHHC11 locus in BL.
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PMC7642379
In the differential expression analysis between adherent and suspension cells, all suspension cell-lines were compared to all adherent cell-lines, and additionally, all pairwise combinations between suspension and adherent cell-lines were evaluated.
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PMC11297139
The representative images supported by the relevant statistics have been chosen upon three independent preparations with similar outcomes.
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PMC9429973
A. Meloni, L. Pistoia, C. Vassalle, S. Maffei, I. Fotzi, R. Rosso, M. Serra, A. Quota, P. P. Bitti, V. Carrai, V. Positano, F. Cademartiri Department of Radiology; Medicina di laboratorio; Endocrinologia Cardiovascolare Ginecologica ed Osteoporosi, Fondazione G. Monasterio CNR-Regione Toscana, Pisa; Centro Talassemie e...
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PMC6222635
Therefore, DWDOP1 and FWDOP1 were further analyzed for structural elucidation.
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PMC8633974
They also had a high encapsulation efficiency of 92.9%.
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PMC7171147
N = 3.
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PMC11783017
In the animal models, the Cis/Mn/NH@PGP hydrogels underwent in situ gelation upon injection and degrades within the tumor microenvironment over time, continuously releasing NHWD-870 and embedded nanoparticles for cisplatin release, thus inhibiting OS tumor growth and metastasis by degrading MYC and modulating the immun...
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PMC11703896
Unfortunately, higher doses can lead to adverse side effects, including immunosuppression, which limits its safe use in clinical settings (Arriola Apelo & Lamming, 2016).
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PMC11612315
For example, research has shown that the GLP-1 receptor agonist liraglutide can alleviate pain hypersensitivity in chronic migraine models by stimulating interleukin-10, suggesting its potential to modulate central sensitization—a crucial mechanism in chronic pain.
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PMC11045125
We next injected NSG mice subcutaneously in the flank with 10 million CB cells (suspended in Matrigel) infected with ΔEBNA2 EBV alone, or the combination of ΔEBNA2 EBV and a Myc expressing vector.
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PMC8711270
Stable LV producer cell line are challenging to develop due to the cytotoxicity of the genes required.
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PMC7022782
The different features observed may be due to a whole homogeneous content for NP constituents for the second type, and to different degrees of diffusion of the negative stain agent inside NPs for the other NP ultrastructures, which could be attributed to a dissimilar distribution of ICG into the polymeric matrix.
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PMC9878562
Removing the C-terminus of ACKR3 causes its plasma membrane localization on all cells, but the truncation renders the receptor incapable to enhance CXCR4-dependent chemotaxis.
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PMC9429973
Here we present a rare and severe variant of Guillain-Barre syndrome that complicated the disease in recovery phase.
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PMC3164356
For each GOslim biological process category, the fraction of all GO terms in that category is shown for human, mouse, rat and CHO genomes.
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PMC11036064
.
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PMC9429973
Anti-tumor efficacy of CYT-338 was evaluated in NSG-mice injected with PB-NK cells and MM cell line MM1S expressing luciferase.
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PMC11435360
We targeted the H2S-producing enzyme, cystathionine -lyase, in 5637 cells using propargylglycine (H2S inhibitor) and performed cytofluorimetric analysis to evaluate cell viability.
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PMC10988555
Herein, we report the unprecedented synthesis and structural characterization of a new family of organometallic Co(III)(η-C5H5) complexes of general formula [CoCp(PPh3)(NN)][(CF3SO3)2], where NN represents 2,2′-bipyridine, 4,4′-dimethyl-2,2′-bipyridine, 1,10′-phenanthroline, and 5-amino-1,10′-phenanthroline ligands.
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PMC7887261
OncoArray genotyping data for 17,878 LCs vs. 13,425 controls; (6) Affymetrix exome array data for 5364 LCs vs. 5724 controls; (7) UK Biobank (UKB) genotyping data for 2166 LCs vs. 401,453 controls.
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PMC11361748
Keratins behave as insoluble proteins in aqueous salt solutions but are readily solubilized in buffers containing a high content of strong denaturing agents such as urea .
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PMC11457557
Hemodynamic alterations, such as increased intra glomerular pressure and hyper filtration, have already been recognized as effective mechanisms in the initiation and development of DKD .
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PMC11746948
GPX4 protein levels detected by immunoblots (m) and relative mRNA levels by RT-qPCR (n) in A375 cells infected with various GPX4 constructs.
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PMC11206251
First, the compounds of the petroleum ether fraction of Illicium simonsii Maxim (PEIM) were identified using LC-MS/MS analysis.
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PMC11576293
Yield 85%; m. p. 228–230°C; H NMR (300 MHz, CDCl3) δ (ppm) = 8.11 (overlap d.d.,
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PMC11335267
Levels of αMSH in arcuate nucleus homogenates from wild type or Alx3-deficient mice (n = 4 per genotype). *
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PMC11727145
Liu et al. demonstrated the importance of insulin in stimulating keratinocyte migration and promoting the formation of epidermal reticular ridges and dermal papillae, although insulin’s effect on keratinocyte migration was found to be independent of EGF presence.
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PMC9577200
Therefore, in this experiment, chitosan, graphene oxide, and GO:CS composite were used to estimate the antibacterial effect against BC and PAO1 by three different methods (turbidity measurement, agar diffusion method, and cell viability loss determination).
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PMC9019892
Data are shown as mean ± SD (n = 2, independent experiments).
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PMC11155445
Delocalization in complexes compared to ligands was proposed based on the increase in bond lengths of thiocarbonyl and carbonyl and the decrease in the bond length of C–N bond.
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PMC11680049
Generally, the callus tissue was of medium density and had a white-yellow color.
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PMC11758416
Incubate culture plate at 37°C for 5 min.g.
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PMC9429973
As all responses were ongoing and early, and MRD analysis was not yet performed.
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PMC8584319
As the eIF4E/4E-BP ratio is an important determinant of the sensitivity to mTOR inhibitors , restoration of 4E-BP1 mRNA expression by refametinib or SB203580 treatment is indicative of the restoration of sensitivity to the mTORC1/2 inhibitor (Figure 6b).
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PMC8662250
Although our data refer to an in vitro model, and further in vivo investigations are needed, they indicate additional potential beneficial effects by CBD against COVID‐19 that integrate with previously established lung protection.
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PMC9250505
F The drug response evaluation: waterfall plot of responses to the vehicle (V), NPB (N), Olaparib (O), or a combined NPB-Olaparib are described by xenograft volume changes (%) from Best Average Response (The minimum value of the average of ΔVolt for t ≥ 10 d); each bar represents an individual PDX.
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PMC8469018
Next, cells were washed once with PBS, resuspended in 100 μL of lysis buffer (Solution 10) enriched with 10 μg/mL DAPI and incubated for 5 min at 37 °C.
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PMC9100622
To further confirm these results, we synchronized cells at the G1/S boundary with double thymidine treatment (Figure S4C).
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PMC11281366
Importantly, viruses produced from miRNA-26a mimic-treated cells displayed an enhanced susceptibility to PGT-121-dependent CML (NHS conditions) as compared to the control (3.1-fold, p = 0.0022) (Figure 6C).
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PMC11295144
The synaptic GABAARs, those more closely associated with NL2 in vivo, show a significantly stronger synergism with NL2 in synaptic initiation and pre-and post-synaptic coupling leading to the formation of fully functional synapses.
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PMC11687167
PCSK9 plays an important role in the proliferation of CD8 + T cells through the MAPK signaling pathway and is involved in the apoptosis of endothelial cells.
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PMC9429973
S. M. Krauß, E. Telzerow, A. S. Moret, D. Richter, M. Rothenberg-Thurley, D. Görlich, M. C. Sauerland, W. E. Berdel, B. Wörmann, U. Krug, J. Braess, P. Heussner, W. Enard, W. Hiddemann, K. Spiekermann, U. Platzbecker, K. H. Metzeler Dept.
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PMC11741577
To this end, a 6 mm surgical incision was made on the dorsal areas of nine-week-old female C57BL/6J mice; then, serum-free IMDM, HMC-1 CM, FSL-1-HMC-1 CM, and FSL-1 (60 μl; 100 μg/ml) were applied after 1 and 3 days.
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PMC8358176
Three weeks before experiments, animals were randomly allotted to three equal groups.
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PMC7917457
The numbers of mice in each group were 8−10.
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PMC11687663
Despite the higher IC50 of A. helferiana extract, its moderate cytotoxicity suggests the presence of bioactive compounds with potential anticancer properties, warranting further investigation.
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PMC11742670
Several libraries and tools are used to develop DL models, including Numpy for numerical computations and array manipulation, Pandas for data handling and analysis, TensorFlow for DL model construction and training, Scikit-Learn for feature scaling, LIME for interpreting and explaining model predictions, and RDKit for ...
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PMC10758402
.
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PMC6771295
In U2OS cells, SIRT3 silencing increased SIRT5 protein levels in untreated and rotenone treated cells (Figure 3Aiii, compare bars 1 and 2 to 5 and 6).
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PMC11190062
Primary cells and cell lines were cultured at 37°C in a humidified atmosphere with 5% CO2.
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PMC11155445
The effect of several substituents on the central thioureide core, including 1-benzoyl, 1-(2-furoyl), 3,3-diethyl, and 3,3-diphenyl, on the electrode performance was analyzed.
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PMC11733919
We have included the senolysis-promoting concentration (100 nM) of drugs to reveal their effects on nonsenescent cells (Figure 3a).
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PMC11310713
The estrous cycle was checked daily for 2 weeks, starting at 10 weeks after HRT.
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PMC11506670
Further, cells expressing G48C, Q43K, and E37K produced spheroids with a larger diameter than wild-type and vector-only controls.
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PMC2944824
Hr has separate domains that enable it to physically interact with receptors for retinoic acid orphan, thyroid hormone, vitamin D, and possibly other receptors , , .
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PMC8750422
Public microarray data containing clinical information were downloaded from the Oncomine database (http://www.oncomine.org, accessed on 3 April 2021).
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PMC10443665
Cytotoxicity of APS, bassorin, and tragacanthin on CCRF-CEM, CCRF-CEM/MTX (cells treated with MTX at IC50), and CCRF-CEM/R cells (CCRF-CEM cells resistant to MTX) was evaluated by MTT assay.
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PMC9848491
The results showed that, compared with the low-risk group, the IC50 of JNK inhibitor VIII, Z.LLNle.
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PMC11453018
Note that iP300w and EWS::FLI1 knockdown both affect the cell cycle and induce cell senescence P300/CBP inhibition and EWS::FLI1 knockdown yield comparable transcriptional changes in ES.
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PMC8903741
Human matrix metalloproteinase 9 (hMMP9) is a biomarker in several diseases, including cancer, and the need for developing detectors and inhibitors of hMMP9 is increasing.
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PMC9429973
In 22 samples with a full dataset, median CSF white cell count was 25/ul (1-233), CSF protein 1.69g/l (0.35-6), CSF IgM 9.49mg/l (1.07-61.5).
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PMC11764090
The SIMYC2 (Table 2) transcription factor may be involved in the cold tolerance induced by MeJA, possibly by improving the antioxidant enzyme system and increasing the levels of proline and lycopene in fruits .
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PMC11610461
Whether PSENEN expression was an independent prognostic target in KIRC patients was examined using a univariate Cox regression model (Dai et al., 2021).
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PMC10538569
Human CD4 T cells transduced with pRRL-GPI-scFv AB65 and cocultured with iDC-HIV-1 AD8 (lower right panel).
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PMC11783017
Subsequently, the cell viability of L929 cells treated with different hydrogel formulations was evaluated by the CCK-8 assay.
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PMC9100622
These data therefore suggest that GGpp is more efficient in complementing Fpp deficiency than the reverse.
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PMC9429973
In particular, the JAK2 V617F ASA-treated platelets presented significant enrichment in platelet activation metabolic and mitochondrial proteins in contrast to the CALR Type I and Type II ET platelets.
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PMC11237030
AQP5 encounters dynamic regulations including trafficking from the cytoplasm to the plasma membrane.
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PMC11536589
The graphs showed the average scores of MGST1 calculated from the group of mice (n = 3). (
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