PMCID
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PMC10748106
The 3D structures of the iridoid glycosides, showing the dipole vector, are displayed in Figure S3.
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PMC11661040
siRNA, lentivirus, and plasmid technologies were applied to modulate gene expression.
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PMC8584666
Therefore, GPI-80 is considered to be a marker for hematopoietic stem cells.
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PMC11471157
This advancement is expected to make substantial contributions to the field of industrial food production.
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PMC10998613
After correlation curves have been fit, initial correlation function amplitudes are used to calculate the fraction of correlated proteins (fc) as shown blow.fc=Gx0maxGG0,GR0, where , , and represent the initial correlation function values for green autocorrelation functions, red autocorrelation functions, and cross-cor...
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PMC9429973
Aims: To characterize second line (2L) treatment patterns and outcomes in patients with R/R MCL initially diagnosed in the rituximab era.
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PMC11367146
Next, the energy minimization process was done with a 10000 steps steepest descent algorithm.
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PMC11721064
The analysis showed that, in addition to the typical expression of Krt10, skin epithelial cells also expressed high levels of stemness-associated genes, whereas corneal epithelial cells exhibited elevated expression of Krt12 (Additional file 1: Fig. S3C).
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PMC11402217
Its antitumor effect is apoptosis-driven, engaging mitochondrial and ER pathways.
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PMC8658661
Thus, a study comparing HepG2 and HepaRG showed that the HB cell line has a greater affinity with normal hepatocytes .
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PMC11678448
In contrast, the levels of the anti-inflammatory cytokine IL-10 were found to be reduced.
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PMC5941560
Instead, they localize to the mitochondria, where they activate a pro-tumorigenic cellular program by regulating mitochondrial transition pore permeability through interaction with cyclophilin D .
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PMC11622247
Fluorogenic hybridization probes are essential tools in modern molecular biology techniques.
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PMC10237474
E-F) Zoom-in spectra of (D) from a deconvoluted mass range of 58600 to 59900 Da shows the full-length protein 4 (E) and 23700–35800 Da shows the clipped species (F). (
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PMC10329237
Resultant ROS can activate transforming growth factor-β (TGF-β), triggering epithelial-mesenchymal transition (EMT) as the main pathological process in IPF (4).
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PMC11301242
These results suggest that genes involved in ecDNA formation may serve as potential therapeutic targets for patients with ecDNA.
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PMC11795610
Ca-free DMEM medium was obtained from Yuchun biology (Shanghai, China).
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PMC11697189
In order to analyze the prediction effect of signature, ROC curve was constructed and the AUC was computed.
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PMC11621493
Experiments were performed with constructs encoding for active proteins or the catalytically dead mS370A mutant as a control.
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PMC11610461
CTLA4 is associated with regulatory T cells (Pedros et al., 2017).
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PMC11615743
To investigate whether the logic gate device allows the identification of target cells from the cell mixture, CCRF‐CEM cells were first stained with Hoechst at 37 °C for 15 min.
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PMC10968586
In ovarian carcinoma HEY cells, 100 μM OLE was not able to affect cell viability, but acted as an antioxidant, decreasing endogenous and erastin-dependent LIP and ROS levels, preventing erastin-dependent ROS accumulation in mytochondria and increasing glutathione peroxidase 4 (GPX4) levels that were reduced by erastin ...
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PMC10523483
We further examined the correlation between H1C and GCLC, GPX2, or GSTO1 in NSCLC samples.
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PMC11714165
After ten days, D‐luciferin was administered intraperitoneally to each mouse, and the luminescence of the tibia was observed using IVIS.
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PMC11413393
PRMT1 is the canonical member of the type I family responsible for the majority of all aDMA species produced in the cell.
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PMC7840816
Data represent mean ± SD from three independent experiments. *
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PMC11549062
During the previous studies, researches about PTP1B mainly focused on diabetes mellitus, because PTP1B is a negative insulin signaling pathway regulator; hence inhibiting PTP1B increases insulin sensitivity and effectively controls diabetes .
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PMC10887351
The antioxidant role of β3-ARs through the UCP2 protein could control the redox homeostasis in ES cells.
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PMC11575040
In this study, LINC00094 was selected as a metformin-suppressed lncRNA to investigate its specific role in melanoma.
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PMC8992508
Unusually, the substitution of the amide function with the ester one led to derivatives that showed low activities on BCRP (EC50 values between 5.9 and 17 μM).
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PMC11687167
Other studies have indicated a possible association between PCSK9 and T-cell function via several mediators.
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PMC10890307
Accordingly, acidosis allowed the remaining cells to survive for more time, potentially allowing for survival until there is a change in the tumor microenvironment.
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PMC6617630
Targeting MYC in the DHL/THL DLBCLs through bromodomain extra-terminal inhibitors (BETi) (JQ1, I-BET, and OTX015) significantly (p < 0.05) reduced proliferation, similar to WT-MYC cells, accompanied by decreased MYC but not BCL2 protein.
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PMC6799808
Cell viability was assessed with trypan blue exclusion assay, and cell proliferation was evaluated with one-solution assay and by cell enumeration, as previously described.
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PMC9429973
Secondary outcomes included initial DI; relative dose intensity (RDI) (i.e., regular-dose drugs delivered six cycles with just 21 days intervals equals 100%); and incidence of unplanned hospitalization, febrile neutropenia (FN), and treatment-related mortality (TRM).
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PMC11545737
Virulence factors that are involved in the activation or regulation of pyroptosis pathways. *
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PMC9429973
The validation cohort of 103 AML patients was obtained from Xuzhou Central Hospital.
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PMC11637276
Scale bar, 200 μm.
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PMC11696576
The TC20 automated cell counter (BIO-RAD, Hercules, California, USA) was used to count the number of cells and check the viability by staining with trypan blue (0.4%) (NanoEnTek, Waltham, MA, USA).
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PMC6487404
REG have a large number of these characteristics; however our data suggest that REG seems not to be a P-gp substrate.
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PMC11064533
Extracellular appendages and adhesins play a critical role in the specific binding of the bacterial cell to receptors on the host cell (6).
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PMC10538569
In contrast, co-culturing iDCs infected with either HIV-1 Mj4 or Yu-2 and CEMss-CCR5-GPI-scFv X5 resulted in decreasing HIV-1 production.
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PMC11718109
The Andor Dragon Fly 200 (Oxford Instruments) inverted microscope equipped with a Zyla cMOS 5.5 camera and controlled by Fusion (Oxford Instruments) software was used for imaging of COS7 cells.
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PMC11697703
Since Fezf2 is essential for specification of corticospinal projection neurons, but not for specification of callosal projection neurons, we compared this putative regulatory region to the Fezf2 chromatin immunoprecipitation sequencing (ChIP-seq) dataset from Lodato and colleagues obtained from cortical progenitors tra...
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PMC11752740
Notably, in SK-BR3 cells, a fraction affected (Fa) of 0.5 indicates a more favorable synergistic interaction between the two treatments (Fig. 5).
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PMC3035438
We have designed gene knock-in constructs to generate DT40 strains that coexpress chimeric human IgG and chicken IgM via B-cell-specific RNA alternative splicing.
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PMC11544122
The purity and concentration of the proteins were verified by SDS page gel stained with Coomassie blue.
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PMC11252553
As the radiation dose escalates from 4 to 12 Gy, the percentages of apoptotic lymphocytes gradually increase in the irradiated samples at both time points.
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PMC11703992
J The percentage of apoptotic cells in Blank-PMA and DDIT4-OE-PMA induced Jurkat cells, evaluated by flow cytometry analysis with Annexin V-FITC/PI staining (n = 3) Comprehensive exploration of the regulation of DDIT4 on apoptosis of T cells.
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PMC11694346
Thus, their evaluation holds significant promise for proactive healthcare management.
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PMC11534035
Treatment with G36 increased expression of PAI1 and increased migration and colony size in CAL39 cells.
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PMC9954564
The model selection and evaluation follow a similar strategy in the MDR v3.0.2 and GMDR v.0.9 methods.
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PMC11323699
TAX2-Cy5 concentration was calculated as described above.
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PMC11335267
Data represent mean ± SEM. *
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PMC11185260
Log-rank analysis demonstrated a statistically significant difference between the survival curves.
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PMC11047729
A study on the human leukemia cells Hl-60, K562, U937, and THP-1 confirmed direct LAPA-induced cytotoxicity.
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PMC11473843
The expression profile of the genes at the top 5% of standard deviation (SD) revealed a clearcut separation between A549 cells treated with XMD8-92 and Seliciclib and the single treatments or control group (Fig. 4A).
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PMC11607764
The associations between PTBP1 expression level and patient survival.
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PMC11592837
Our results with ETP and DOX point to a much faster DDR in Jurkat cells compared to PBMCs.
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PMC11347429
In addition, exploring whole-blood gene expression provides a valuable avenue for developing clinical tests for point-of-care use, enabling a more personalized and effective approach to patient management.
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PMC11574271
E A diagram illustrating the specific binding site of miR-659-3p within the 3′UTR region of RON mRNA, along with a mutated version of the RON 3′UTR, is presented.
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PMC10667689
Also, G6PD showed increased expression at the transcriptional level in both cell lines compared to the control and PMA group (G, H) (*p < 0.05, **p < 0.01, ***p < 0.001).
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PMC11721096
The methanol extract of A. sparsifolia (83 g) was applied to a silica gel column (100–200 mesh) eluted by dichloromethane–methanol (from 50:1 to 0:1) to afford eight fractions.
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PMC11666785
An additional image is reconstructed from all the single molecules collected in 2-4 consecutive frames.
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PMC11687663
It is noteworthy to mention that, the presence of carbonyl group (C = O, stretching) is supported by the strong absorption peak in the range of 1703 to 1627 cm.
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PMC11066331
6 Three-dimensional visualization of PCA of 175 mass spectra recorded from 7 binary cell population mixtures (A).
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PMC8711270
Simple retroviruses contain two separate populations of viral RNAs, where viral genomic RNA is distinct from mRNA.
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PMC11075223
Yixue Guangbiji and Mobao Zhaiji Yanfang both mentioned that the prescriptions containing Cimicifugae Rhizoma had the advantage on dysentery and metrorrhagia during the Ming Dynasty .
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PMC3681794
All graphs show the mean absorbance relative to the untreated controls against log drug concentration and are the mean of 3 independent experiments ± SEM.
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PMC6600592
Colorless crystal, yield 89.5%.
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PMC11045125
Of note, the P493-6 cell line expresses much lower levels of some BL-associated (and GC B cell expressed) cellular proteins in comparison to the N1, N3 and N4 stable cell lines derived from the ΔEBNA2 + Myc lymphomas (Fig 8B), including CD10, GCSAM, BACH2, and CD179B (IGLL1).
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PMC11659921
Prognostic models were significantly associated with the immune microenvironment of OV. (
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PMC11682172
The stimulating effect of T lymphocyte conditioned medium (CM) was grossly similar for all three cell lines and less than the effect of direct T cell contact.
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PMC11694625
Then, the appropriate boxes for the interactions of ligand and receptor were set and the set parameters were saved as conf files.
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PMC11301242
Among them, 6 lncRNAs were found to be amplified in all 23 AML patients with MYC-containing amplicons and in the AML cell line HL60.
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PMC8414691
Scale bar = 10 μm.
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PMC11804588
The authors also developed single-cell FOODIE (scFOODIE) to enable TF footprint analysis in heterogeneous cell populations.
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PMC7294030
Initiator caspases, such as caspase-1, -2, -8, -9, and -10, initiate a proteolytic cascade while activated caspases, such as caspase-3, -6, and -7, are associated with cleavage of specific intracellular substrates (focal adhesion kinase, poly-ADP-ribose polymerase) (Salvesen and Dixit, 1997; Nicholson and Thornberry, 1...
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PMC4005030
It is present in products of plant origin, such as olive oil or white wine .
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PMC8345486
The OVCAR-3 cell line was cultured in high-glucose RPMI 1640 (ATCC modification medium) with 10% FBS and 100 μg/mL penicillin/streptomycin.
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PMC10748106
Geniposide exhibited no activity in these cell lines.
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PMC11653533
F. carica, traditionally used for constipation and respiratory ailments, demonstrates anti-inflammatory and neuroprotective effects, while F. exasperata has shown promising hypoglycemic and hepatoprotective activities, validating its use in traditional medicine for managing blood glucose levels and liver health (Kingsl...
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PMC11746948
g Immunoblot confirmation of APT2 and GPX4 expression in A375 cells with APT2 knockdown, infected with HA-APT2 lentivirus.
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PMC11279397
These analyses provide theoretical support for the antiviral activity of Perilla frutescens and serve as a reference for further research on its pharmacological effects and the development of related drugs.
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PMC9429973
In our study we found a median younger age of the patients at the moment of the diagnostication (median age of 63 years old) compared with the literature and a ratio male:femato 2:1.
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PMC8073654
Phenylbutenoids and curcuminoids have been mainly isolated from Z. cassumunar as a single compound, and its essential oil containing monoterpenoids, sesquiterpenoids, and phenylbutenoids has been assessed primarily for its pharmacological and cosmeceutical use.
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PMC6803987
On P5, the right lobes were fixed with 4% paraformaldehyde and sent to the pathology department of the Affiliated Hospital of Guilin Medical College for pathological section, including paraffin embedding, sectioning, staining (hematoxylin-eosin staining), and observation of pathological changes by optical microscopy.
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PMC11486946
In addition to the detection of EGFR-TKIs, our screening detected non-EGFR-TKI compounds (i.e., broxyquinoline, daunorubicin, eltrombopag, sorafenib, verteporfin, glafenine, and nilotinib), none of which are known to act on EGFR directly.
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PMC11216402
The Mes-like GC cell lines have a higher similarity to fibroblast than the primary tumors, correlations being as high as 0.8.
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PMC11529598
For cell invasion experiments, cells were seeded into the upper chamber of a Boyden chamber coated with Matrigel (BD) at a density of about 2 × 10 cells per well in serum-free DMEM.
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PMC11085211
Indeed, the cell line GIST-48b is characterized by the absence of KIT expression, supporting the hypothesis that the mechanism is independent from KIT, possibly paving the way for developing therapeutic strategies other than TKIs in imatinib resistance.
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PMC11761919
The Fiber2-expressing E. faecalis culture without any additives as a control.
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PMC11806182
MicroRNAs (miRNAs) could provide insights into these mechanisms and reveal potential therapeutic targets.
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PMC8469018
IC50 value for teriflunomide was determined using the AAT Bioquest IC50 calculator.
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PMC11763126
C KEGG pathway analysis.
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PMC11269933
All data are presented in the form of mean ± SD.
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PMC8992508
Acryloylphenylcarboxamide derivatives In the same year, the same research group, reported another series of acryloylphenylcarboxamides [Figure 6] and of acryloylphenylcarboxylates with a 4’-methoxy group on ring A of the chalcone.
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PMC11411131
FLAG-TrxR1-HA, FLAG-SelN, Tau, FLAG-SelP-HA, and TetR-Sec-Krab were inserted into the multiple cloning site in the pCI-pur vector.
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PMC11707577
Many viral structures have been used as template for material synthesis and assembly, , including cowpea chlorotic mottle virus (CCMV), , cowpea mosaic virus (CPMV), tobacco mosaic virus (TMV) and M13 bacteriophage.
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PMC11627398
Xu et al. reported that compared to sepsis patients in the survival group, whole blood copper levels were notably elevated in the death group of sepsis patients , suggesting that high copper levels are associated with the poor prognosis in sepsis patients.
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