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This study was supported by the "Hepatibivax" grant from the "Emergence" program of the Agence Nationale pour la Recherche (ANR, France , grant ANR-11-EMMA-026 ), the Agence Nationale pour la Recherche sur le SIDA et les hépatites virales (ANRS, France , grant 2014-1 ), the Ligue contre le Cancer ( Comités de l...
10.1371/journal.pone.0151626
article
en
2,016
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Medicine
https://openalex.org/fields/27
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Background: Interleukin 6 (IL-6) signaling plays a key role in the pathophysiology of rheumatoid arthritis (RA) and is inhibited by sarilumab, a human monoclonal antibody blocking the IL-6 receptor alpha (IL-6Rα). The effects of sarilumab plus methotrexate (MTX) on serum biomarkers of joint damage and bone resorption w...
10.1186/s13075-016-1132-9
article
en
2,016
false
false
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Medicine
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The efficacy and safety of sarilumab in combination with methotrexate (MTX) in patients with moderate-to-severe RA and inadequate response to MTX (MTX-IR) were evaluated in the two-part (phase II (part A) and phase III (part B)) MOBILITY trial (NCT01061736) [24, 26]. In MOBILITY A and B, patients treated with sariluma...
10.1186/s13075-016-1132-9
article
en
2,016
false
false
false
true
Medicine
https://openalex.org/fields/27
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Study design The results of MOBILITY (NCT01061736), a two-part (phase II (part A) and phase III (part B)), randomized, double-blind, placebo-controlled, multicenter study that evaluated the efficacy and safety of subcutaneous sarilumab in combination with MTX in patients with active RA and MTX-IR have previously been d...
10.1186/s13075-016-1132-9
article
en
2,016
false
false
false
true
Medicine
https://openalex.org/fields/27
cc-by
Sarilumab 200 mg q2w was chosen for these analyses because this dose demonstrated better efficacy compared with sarilumab 150 mg q2w with respect to the bone and joint x-ray outcomes (i. e. , mTSS, ES, and JSN) in MOBILITY part B, and the additional biomarkers measured reflect pathological processes associated with the...
10.1186/s13075-016-1132-9
article
en
2,016
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false
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false
Medicine
https://openalex.org/fields/27
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An unstructured covariance structure was assumed in all models. The Bonferroni correction was used to adjust P values for multiplicity. A P value <0. 05 after adjustment was considered significant. For exploratory purposes, percent changes from baseline in biomarkers and sRANKL/OPG were also compared between responders...
10.1186/s13075-016-1132-9
article
en
2,016
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true
false
false
Medicine
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Acknowledgements This study was sponsored by Sanofi and Regeneron Pharmaceuticals, Inc. Editorial assistance was provided under the direction of the authors by Gretchen Chidester, PhD , MedThink SciCom , and funded by Sanofi and Regeneron Pharmaceuticals, Inc. The authors thank Dr Emmanuelle Cousin , Ling Cai ,...
10.1186/s13075-016-1132-9
article
en
2,016
false
false
true
false
Medicine
https://openalex.org/fields/27
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Tolerability was also assessed in a phase I trial in patients with advanced solid tumors and hematological neoplasms (NCT00559533), and results are pending. Based on their mechanisms of action, RG7112 and other agents in this class might be effective in patients with MDM2 amplification and wild-type TP53 tumor. However...
10.1158/1078-0432.ccr-15-1015
article
en
2,015
false
false
false
true
Medicine
https://openalex.org/fields/27
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Statistical analysis All statistical data were collected using GraphPad Prism software (San Diego, CA). For protein and RNA level comparisons, p-values were calculated by comparing data measured from 3 independent experiments from each individual cell line with data from all the other cell lines. Parametric analysis wa...
10.1158/1078-0432.ccr-15-1015
article
en
2,015
true
true
false
false
Medicine
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MDM2 amplification does not impact prognosis in newly diagnosed GBM patients and is maintained at tumor recurrence Data sets from the 2013 GBM TCGA database [4] (publically available on cBio portal; MSKCC) were extracted and analyzed. TP53, MDM2 and MDM4 were found to be genetically altered (i. e. gene copy number or...
10.1158/1078-0432.ccr-15-1015
article
en
2,015
true
false
false
false
Medicine
https://openalex.org/fields/27
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PDCLs maintain genetic TP53 status heterogeneity observed in human GBM tumors Thirty-six GBM cell lines including 32 PDCLs from two sets (cohort #1 and cohort #2, 4/32 PDCLs were used in both cohorts) and 4 commercially available cell lines were used in the present study. Data acquired from both cohorts were obtained f...
10.1158/1078-0432.ccr-15-1015
article
en
2,015
true
false
false
false
Medicine
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Finally, RG7112 appeared to be inactive in a TP53 mutant model (4339). Taken together, these observations correlate with the data observed in vitro. The efficacy of RG7112 (100 mg/kg) was then assessed in the MDM2-amplified 3731 orthotopic GBM in vivo model. RG7112 decreased tumor progression rate as shown by biolumine...
10.1158/1078-0432.ccr-15-1015
article
en
2,015
true
false
false
false
Medicine
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In the clinic, RG7112 treatment of MDM2-amplified liposarcoma patients resulted in a stable disease (14 out of 20 patients) or a partial response (1 out of 20 patients) [11]. The data reported here in the orthotopic model is coherent with this observation, as tumor growth was inhibited by the treatment, but seemed to ...
10.1158/1078-0432.ccr-15-1015
article
en
2,015
true
false
false
false
Medicine
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Acknowledgments Funding information: This work is part of the GlioTex (i. e. , Glioblastoma and Experimental Therapeutics) project funded by La Fondation ARC pour la Recherche sur le Cancer and supported by L'Institut Universitaire de Cancérologie (IUC). Additional support for this work was received from the DFCI...
10.1158/1078-0432.ccr-15-1015
article
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2,015
false
false
true
false
Medicine
https://openalex.org/fields/27
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We thank the Harvard ICCB-Longwood Screening Facility for assistance with screening. MRI data were acquired by the Center for Neuroimaging Research (CENIR) who has received funding from the program " Investissements d'avenir " ANR-10-IAIHU-06 , from the Ile-de-France Region ( DIM Cerveau et Pensée ), and from I...
10.1158/1078-0432.ccr-15-1015
article
en
2,015
false
false
true
false
Medicine
https://openalex.org/fields/27
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In addition, serum 25(OH)D concentrations or HOMA-IR were included in the models, as covariates, according to the dependent variable. The effects of the covariates were assessed by the values of the regression coefficients corresponding to the non-standardized regression coefficients (nSRC). IBM SPSS Statistics, versio...
10.3889/oamjms.2016.131
article
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Acknowledgements We would like to acknowledge all children and parents who participated in the study. Great thanks to the nurses and physicians of the AGREXAM Health Centre , to the teachers of physical education, to Mrs L Nesty , the headmaster of the middle school named " College Saint John Perse " located in Guade...
10.3889/oamjms.2016.131
article
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2,016
false
false
true
false
Medicine
https://openalex.org/fields/27
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The jury remains out on the most appropriate ICD, algorithm and programming for ICD patients, including the most appropriate boundaries of the VT zone. In the initially published OPTION trial (ClinicalTrials. gov-NCT00729703, date of registration: August 4, 2008) 7 therapy with DC settings for ICD discrimination comb...
10.1038/srep21748
article
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2,016
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false
false
true
Medicine
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Methods The design and main results of the OPTION trial have been published 7, 8. In brief, this prospective, randomised (1:1), multicentre, single-blinded (patients), parallel-group trial enrolled 462 patients eligible for ICD therapy for primary or secondary prevention of sudden cardiac death (left ventricular eject...
10.1038/srep21748
article
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2,016
false
true
false
false
Medicine
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Rates of patients with appropriate and inappropriate shocks at two years of follow-up were obtained from the OPTION database. All reported shocks in the OPTION trial were validated by a blinded events committee of 5 experts who analysed the electrographic recordings in the device memories 7. Data on shocks were obtain...
10.1038/srep21748
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Medicine
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Sorin, St. Jude Medical. Dr. D. Babuty has received travel support from Biotronik, Boston Scientific, Medtronic, Sorin and St. Jude Medical; he has performed clinical studies supported by Biotronik, Medtronic, Sorin and St. Jude Medical. Dr. P. Sick has received a research grant from Sorin. Dr. J. M. Davy has received ...
10.1038/srep21748
article
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2,016
false
false
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false
Medicine
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Acknowledgements The authors are indebted to Pelle Stolt PhD for editorial assistance in the preparation of this manuscript. The Option study was supported by Sorin CRM, Clamart, France.
10.1038/srep21748
article
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2,016
false
false
true
false
Medicine
https://openalex.org/fields/27
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Method Participants. Fifteen observers (eight female) participated in Experiment 1. 1 Participants were recruited from Utrecht University. All participants were between 18 and 23 years of age and reported normal or corrected-to-normal vision. All participants signed informed consent before participating and received m...
10.1167/16.12.363
article
en
2,016
false
true
false
false
Neuroscience
https://openalex.org/fields/28
cc-by
This research was funded by VIDI Grant 452-13-008 from the Netherlands Organization for Scientific Research to Stefan Van der Stigchel , by European Research Council Consolidator Grant 615423 awarded to CNLO, and by the People Programme ( Marie Curie Actions ) of the European Union's Seventh Framework Pro...
10.1167/16.12.363
article
en
2,016
false
false
true
false
Neuroscience
https://openalex.org/fields/28
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Participant data, as well as experimental and analyses scripts, are available from https://bitbucket. org/smathot/p0014. 3-pupil-size-and-working memorysame-different/ This document is copyrighted by the American Psychological Association or one of its allied publishers. This article is intended solely for the personal...
10.1167/16.12.363
article
en
2,016
false
true
false
false
Neuroscience
https://openalex.org/fields/28
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Epidemiological assumptions and data Survival (S) was modeled based upon the Collaboration of Observational HIV Epidemiological Research Europe (COHERE) study 4 and extrapolated to a 5-year time (t) horizon as a constant hazard. 5 No ethical permission was required for this study, as the COHERE study data was previou...
10.2147/hiv.s96713
article
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2,016
true
false
false
false
Medicine
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Older adults (. = 50 years) diagnosed late were 14 times more likely to die within a first year of diagnosis compared to older adults diagnosed early. 18 he assumptions that 14% of all newly-diagnosed HIV infections occur in individuals aged over 50 years and that 64% of these are males were combined with life expectan...
10.2147/hiv.s96713
article
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2,016
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false
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false
Medicine
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Cost impact calculations In order to calculate the total medical care costs incurred for the cohort under the current and future scenarios, each year of survival of an individual was multiplied by the appropriate annual cost. The annual cost is the sum of all categories of HIV clinical care from a payer perspective and...
10.2147/hiv.s96713
article
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2,016
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false
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false
Medicine
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Results We modeled large-scale resting state brain dynamics using simple linear diffusion of noise over the anatomical structure. This is the well known Ornstein-Uhlenbeck model as described for example in [35] , where Galán applied this model to investigate micro-scale networks. To test the results against empirical ...
10.1371/journal.pone.0157292
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Data collection Structural data from DTI and resting-state BOLD signal time series were acquired for 14 healthy subjects (age between 25 and 33 years old, 6 females). Data can be found in S1 Folder. A detailed description of the generation of SC and FC matrices from those data can be found in [46]. Empirical data were...
10.1371/journal.pone.0157292
article
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mgz). 3. WM and subcortical segmentation, cortical tessellation generating GM-WM and GM-pia interface surface-triangulations, probabilistic atlas based cortical parcellation, e. g. , using Desikan-Killany (DK) atlas [56]. This generates aparc+aseg. mgz volumes that contain all parcellated regions (cortical and subcort...
10.1371/journal.pone.0157292
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2,016
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After this step, FSL's FEAT pipeline is used to perform the following operations: deleting the first five images of the series to exclude possible saturation effects in the images, high-pass temporal filtering (100 seconds high-pass filter), motion correction, brain extraction and a 6 DOF linear registration to the MNI...
10.1371/journal.pone.0157292
article
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Medicine
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Any pre-processing technique, which implies a normalization of data must be avoided when using our analytic operation, for this reason it is important to stress that we did not perform global signal regression on data. Global regression, in fact, changes the distribution of the eigenvalues of the FC and, in particular,...
10.1371/journal.pone.0157292
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Medicine
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Acknowledgments We wish to thank the anonymous reviewers for providing helpful comments and references which helped to improve the manuscript. We are also grateful to Demian Battaglia and Tommaso De Lorenzo for helpful discussion and to Enrique Hansen for providing the code for the non-linear model.
10.1371/journal.pone.0157292
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Since the first phase was used for identification, this correction was applied to the number of variants tested in the meta-analysis. When several SNPs located on the same gene were significantly associated with ACPA or erosion, haplotypes were built and analysed. Haplotypes were assigned to each individual using PLINK...
10.1136/rmdopen-2015-000226
article
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Medicine
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Furthermore, an SNP located on RANKL (rs7325635) had a trend for significant association with erosions with a p=0. 06 on the χ 2 test and a p value of 0. 05 on the Cochran-Armitage test. Thus, it was also added to the meta-analysis. Association between the SNPs located on RANK, RANKL and OPG and ACPA or erosions in the...
10.1136/rmdopen-2015-000226
article
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05). Interactions between the SNPs and between SNPs and HLADRB1*SE on ACPA presence and erosions were tested. No interaction was significant after using three different methods (data not shown).
10.1136/rmdopen-2015-000226
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Tan et al 10 further showed that rs7984870 located on the promoter of RANKL modulated the expression of RANKL via activated T cells only in rheumatic factor positive patients with RA. In a recent study, Knevel et al 9 identified an SNP (rs1485305) on OPG associated with radiographic progression in a meta-analysis of 4 ...
10.1136/rmdopen-2015-000226
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Medicine
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Acknowledgements The authors thank Nathalie Rincheval for expert monitoring and data management; S Martin who did all the central dosages of CRP, IgA and IgM rheumatoid factor and anti-CCP antibodies; the Biological Resources Centre (Paris-Bichat , J Benessiano ) which was in charge of centralising and managing b...
10.1136/rmdopen-2015-000226
article
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2,016
false
false
true
false
Medicine
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Funding This work received a grant from the French Society of Rheumatology. An unrestricted grant from Merck Sharp and Dohme (MSD) was allocated for the first 5 years of the ESPOIR cohort. Two additional grants from INSERM were obtained to support part of the biological database. The French Society of Rheumatolog...
10.1136/rmdopen-2015-000226
article
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2,016
true
false
false
false
Medicine
https://openalex.org/fields/27
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e. large nanoparticles while the second component is a priori assigned to slowly relaxing nanoparticles, i. e. with intermediate size. But at this stage, different scenarios can be considered and it remains difficult to model the hyperfine structure from experimental data coming from the measurements obtained at one te...
10.1038/srep26550
article
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2,016
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false
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false
Nursing
https://openalex.org/fields/29
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Purified ferritin-M6A was crystallised using the sitting-drop vapour-diffusion method at 293 K. 0. 5 mL ferritin-M6A (20 mg mL -1 ) and 0. 5 mL reservoir solution (0. 1 M Na-cacodylate pH 6. 5, 0. 2 M magnesium acetate and 30% MPD) were mixed to form the drop. Crystals were harvested and flash-cooled in liquid nitrogen...
10.1038/srep26550
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Nursing
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The final model was built by Coot 31 and refined in REFMAC 32. For Rfree calculation, 5% of the data were excluded. Structural figures were prepared with PyMOL 33. RMS calculations were performed with SwissPDB viewer 34 over all Cα atoms using the domain alternate fit to align structures on the basis of the conserv...
10.1038/srep26550
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Nursing
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The sample-to-detector (Aviex CCD) distance was set to 1892 mm, covering a q-range of 0. 004-0. 7 Ǻ -1. All experiments were temperature controlled at 25 °C. Typically, 55 successive frames of 0. 5 s each were recorded for both protein solution and its corresponding buffer. Each frame was first angularly averaged and t...
10.1038/srep26550
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Nursing
https://openalex.org/fields/29
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5 μ g mL -1 ) was added to the cell culture medium to select for stably transduced cells. The cells were incubated at 37 °C in a humidified atmosphere of 95% air and 5% CO 2. Construction of C6-pEIRES-HA-ferritin and C6-pEIRES-HA-ferritin-M6A cells. The murine ferritin h-chain cDNA (GenBank accession no. NM-010239) wit...
10.1038/srep26550
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Nursing
https://openalex.org/fields/29
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Acknowledgements We thank ESRF (Grenoble, France ) beamline ID14-4 and SOLEIL beamline SWING for assistance during data collection. We also thank Boaz Shaanan and Anat Shahar ( MCRC unit ) for their advice during X-ray data collection and processing, and to Ben A. Friesem , Yael Kalisman , Sharon G. Wolf and ...
10.1038/srep26550
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Nursing
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2 ). We also attempted to transfect into the mosquito-transmissible NF54 wild type background on three separate occasions without success (data not shown). Proceeding with the two lines in the D10 background, we observed both Pf apiG3PAT (À) lines were viable and grew normally as determined by frequency of subculturing...
10.1111/cmi.12633
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2,016
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false
Medicine
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Pb apiG3PAT is targeted to the apicoplast and expressed in liver stage parasites Pb apiG3PAT shares strong sequence similarity with Pf apiG3PAT and is likewise predicted to possess an apicoplast targeting sequence at its N-terminus (Supplementary Fig. 1 ). To verify that Pb apiG3PAT is targeted to the apicoplast, we us...
10.1111/cmi.12633
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2,016
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Medicine
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1% polyethyleneimine. Images were acquired using a Leica SP2 inverted confocal microscope at ambient temperature, and merged and contrast adjusted using ImageJ software (NCBI). Immunofluorescence microscopy of Pf apiG3PAT 1-70 gfp parasites was performed by fixing in paraformaldehyde/ glutaraldehyde and permeabilizing ...
10.1111/cmi.12633
article
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2,016
false
true
false
false
Medicine
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, 2012). Briefly, PCR-free libraries were prepared using NEBNext DNA Library reagents (NEB) and NEXTflex DNA Barcodes (Bioo Scientific). Eight libraries were multiplexed with 8% PhiX control and run across two lanes on the Illumina HiSeq 2500 system using single-end sequencing. Sequencing data was analysed using Galaxy...
10.1111/cmi.12633
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2,016
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Pellets were re-extracted using methanol:water (2:1 v/v), and the second supernatants were then combined with the first extract and dried then stored at À80 C. Prior to GC-MS, extracts were partitioned into chloroform:methanol:water (1:3:3 v/v) and the apolar (lipid) phase was dried, derivatized to fatty acid methyl es...
10.1111/cmi.12633
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false
Medicine
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Live imaging of liver stage parasites was performed 48 h post-infection. Cells were stained briefly with 5 μg ml À1 Hoechst 33342 (Life Technologies), then imaged using a Leica SP5 inverted laser scanning confocal microscope in a 37 C temperature controlled chamber. Images were taken sequentially for the two channels a...
10.1111/cmi.12633
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Cells were fixed as above and labelled with mouse anti-HSP70 (gift from Moriya Tsuji), goat antimouse Alexa Fluor 488 (Molecular Probes) and DAPI, before mounting in Fluorescence Mounting Medium (DAKO). Imaging was performed using an OMX V4 Blaze in wide field deconvolution mode, with z-slices acquired across the entir...
10.1111/cmi.12633
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Acknowledgements MJS was supported by an Australian Postgraduate Award. GIM gratefully acknowledges a Program Grant from the National Health and Medical Research Council (Australia) and a Discovery Grant from Australian Research Council. CYB was supported by ANR-RPDOC/ Atip-Avenir CNRS/ Finovi Grant/Labex Parafr...
10.1111/cmi.12633
article
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2,016
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false
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Medicine
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Methods We performed a systematic review of the relevant literature, in accordance with the PRISMA guidelines. In PubMed database we used the following terms: "macrocephalic sperm", "enlarged head spermatozoa", macronuclear spermatozoa", "sperm head measurements", large-head spermatozoa". Only studies with a fully desc...
10.1186/s12610-016-0035-6
review
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The 4 geomorphologic landscapes were labelled l = 1 (Coastal Plain), l = 2 (Northern Chain), l = 3 (Central Massif) and l = 4 (Inini-Camopi chain) to indicate their position on the North-South gradient that spread from the Atlantic coast to the Amazonian frontier with Brazil. Insects were captured using light traps lo...
10.1371/journal.pntd.0004427
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The sets of parameters of all models used to analyse SD and ITc data were identified by applying the equal/unequal relationships in the exact same way as for the A data. A one by one description of the 15 hypotheses and models applied to A, SD and ITc data can be found in Table A in S2 Table. Fitting the models to dat...
10.1371/journal.pntd.0004427
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Infection by T. cruzi (ITc). We fitted the models to the ITc data for each triatomine species. The vector X l then contained a unique statistical variable I s,l corresponding to the expected number of infected individuals of species s in landscape l, O l contained the observed number i s, l of infected individuals of s...
10.1371/journal.pntd.0004427
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The second step in the model selection approach consists of comparing the 15 models according to their ability to fit to the data (as measured by the LLH obtained for the best fits) and with respect to the number of their parameters. Such comparisons can be made using the standard Akaike Information Criterion (AIC) tha...
10.1371/journal.pntd.0004427
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Geographical distribution of triatomines and their infection by T. cruzi The distribution of triatomines in French Guiana was investigated by collecting insects in 22 sites covering most of this territory (Fig 1A ). A total of 731 adult specimens were captured during 256 nights of trapping. About 68. 7% were males and ...
10.1371/journal.pntd.0004427
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This work has benefited from an " Investissements d'Avenir " grant managed by Agence Nationale de la Recherche (CEBA, ref. ANR-10-LABX-25-01 ). This work was performed within the framework of the LABEX ECOFECT ( ANR-11-LABX-0048 ) of Université de Lyon , within the program " Investissements d'Avenir" ( ANR-11-IDE...
10.1371/journal.pntd.0004427
article
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false
Medicine
https://openalex.org/fields/27
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ACKNOWLEDGMENTS The publication fee was supported by the Région de Bourgogne (Contract 9201AAO050S02953 ) and the Fonds Européen de Développement Régional (FEDER).
10.3389/fphys.2016.00613
review
en
2,016
false
false
true
false
Medicine
https://openalex.org/fields/27
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Structural modeling and molecular dynamics simulation studies of MCs The three-dimensional (3D) structures of the four MC constructs (MC75, MC86, MC75S and MC86S) were predicted using the internal coordinate program JUMNA (47). In particular, a closed, planar circular DNA molecule was created using the standard value ...
10.1093/nar/gkw651
article
en
2,016
false
true
false
false
Immunology and Microbiology
https://openalex.org/fields/24
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Analysis of minicircle curvature In order to define whether the major groove a given location along the MC is facing toward the inside or the outside of the MC, we studied the local curvature of the predicted MC structures, using a procedure that uses the helical axis calculated by the Curves+ program (62, 63). First,...
10.1093/nar/gkw651
article
en
2,016
false
true
false
false
Immunology and Microbiology
https://openalex.org/fields/24
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Mapping of integration sites and correlative studies In order to identify the absolute position of the integration sites for each sequence of the 24 libraries, a custom python script was implemented to read these sequences and count, for each position within the region of interest, the number of integrations achieved. ...
10.1093/nar/gkw651
article
en
2,016
false
true
false
false
Immunology and Microbiology
https://openalex.org/fields/24
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ACKNOWLEDGEMENTS We thank M. Naughtin , F. Di Nunzio , P. Charneau and Céline Ralec for scientific discussions and L. Ma and C. Calmels for technical help. High-throughput sequencing has been performed on the Genomics Platform, member of 'France Génomique' consortium. We acknowledge GENCI for computer time...
10.1093/nar/gkw651
article
en
2,016
false
false
true
false
Immunology and Microbiology
https://openalex.org/fields/24
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FUNDING ANRS [ AO2014-02 to V. P. , M. L. ] and [ AO2012-02 to M. R. and J. B. ]; SIDACTION [AI25-1-02332 to M. P. , A. J. , M. R. , R. L. , M. L. ] and to M. R. and J. B. ; ANR, Genomics Platform, member of 'France Génomique' consortium [ ANR10-INBS-09-08 to C. B. ]; French Infrastructure for Integrated Stru...
10.1093/nar/gkw651
article
en
2,016
false
false
true
false
Immunology and Microbiology
https://openalex.org/fields/24
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abscessus (27). Further study found that some STB isolates lacked the insertion element IS1081, while a new ISMycA1 (GenBank accession number AJ619854) was discovered in the "M. canettii" CIPT140010059 genome (12). ISMycA1 encodes a transposase which, surprisingly, shares 48% amino acid sequence identity with IS-enco...
10.3389/fpubh.2015.00283
review
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true
false
false
false
Medicine
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ACKnOwLeDGMenTS This study was financially supported by URMITE, IHU Méditerranée Infection, Marseille, France.
10.3389/fpubh.2015.00283
review
en
2,016
false
false
true
false
Medicine
https://openalex.org/fields/27
cc-by
A, mc 2 or mc 2 ::pKirT-pstP cultures were initiated at an A 600 of 0. 1 and grown in the absence or the presence of 50 ng/ml ATc for 12 h. Whole cell lysates (WCLs) were prepared, and 35 g (for PstP) and 20 g (for GroEL1) of lysates were resolved on SDS-PAGE and probed with ␣-PstP and ␣-GroEL1 antibodies. B, mc 2 or m...
10.1074/jbc.m116.754531
article
en
2,016
false
true
false
false
Biochemistry, Genetics and Molecular Biology
https://openalex.org/fields/13
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The data were plotted using GraphPad Prism version 6 software. To enumerate cfu at 0 and 30 h, cultures were harvested, washed twice with PBST (PBS containing 0. 05% Tween 80), resuspended, and serially diluted, and different dilutions were plated on 7H10 agar plates containing OADC. In the case of M. tuberculosis (Rv)...
10.1074/jbc.m116.754531
article
en
2,016
true
true
false
false
Biochemistry, Genetics and Molecular Biology
https://openalex.org/fields/13
cc-by
Acknowledgments-We thank the Tuberculosis Aerosol Challenge Facility (TACF) of International Center for Genetic Engineering and Biotechnology, New Delhi (ICGEB) for support in all animal experiments. We thank Dr. Dhiraj Kumar for helpful discussions and for allowing use of the TACF facility. We thank the scanning e...
10.1074/jbc.m116.754531
article
en
2,016
false
false
true
false
Biochemistry, Genetics and Molecular Biology
https://openalex.org/fields/13
cc-by
* This work was supported by Indo French Centre for the Promotion of Advanced Research Grant 5303-1 (to V. K. N. , Y. S. , and V. M. ) and BSC0104 (CSIR) (to Y. S. ).
10.1074/jbc.m116.754531
article
en
2,016
false
false
true
false
Biochemistry, Genetics and Molecular Biology
https://openalex.org/fields/13
cc-by
Study Design and Setting Data from an international, multicenter, and cross-sectional MusiQoL validation study were used. 20 Patients were recruited between January 2004 and February 2005 at neurological departments in 15 countries: Argentina, Canada, France, Germany, Greece, Israel, Italy, Lebanon, Norway, Russia, Sou...
10.1097/md.0000000000003068
article
en
2,016
true
false
false
false
Medicine
https://openalex.org/fields/27
cc-by
Strengths and Limitations A limitation in our study is that we used the entire sample only for the MIRT model calibration. MCAT simulations were performed using only the complete response patterns. To overcome this issue, it should be possible to use a well-known data imputation method, such as the multiple imputations...
10.1097/md.0000000000003068
article
en
2,016
true
false
false
false
Medicine
https://openalex.org/fields/27
cc-by
ACKNOWLEDGMENTS We thank A. Bendelac , J. Y. Bertrand , and D. Guy-Grand for critical reading. We are thankful to C. Possot to have started the breeding of the different mouse lines. We acknowledge the Center for Human Immunology and Cytometry platform at Institut Pasteur for support. This work was supported by...
10.1016/j.celrep.2016.01.015
article
en
2,016
false
false
true
false
Immunology and Microbiology
https://openalex.org/fields/24
cc-by
Developing eCASH The guiding principles of eCASH are clear and components of the eCASH concept are supported by current guidelines; details of this philosophy of care can be expected to evolve in response to clinical trial data. Among trials currently in progress that may be expected to influence the final form of eCAS...
10.1007/s00134-016-4297-4
review
en
2,016
false
false
false
true
Medicine
https://openalex.org/fields/27
cc-by
Acknowledgments Orion Pharma (Espoo, Finland) initiated and supported the round-table meeting from which this work emerged, but had no influence on the development of the views expressed. Writing and editorial assistance was provided by Hughes associates, Oxford, UK.
10.1007/s00134-016-4297-4
review
en
2,016
false
false
true
false
Medicine
https://openalex.org/fields/27
cc-by
Acknowledgements S. Mirrahimi has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (grant agreement No 639638 ), and from the french ANR projects KIBORD ANR-13-BS01-0004 and MODEVOL ANR-13-JS01-0009.
10.1016/j.crma.2016.12.001
article
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2,016
false
false
true
false
Biochemistry, Genetics and Molecular Biology
https://openalex.org/fields/13
cc-by
Acknowledgments: We would like to thank the Ebola-Tx consortium and all those (authorities, CNTS staff, ETC staff, all MSF staff, laboratory staff, communities, and Ebola survivors) that have contributed to the implementation of the Ebola-Tx. Our gratitude goes to the Clinical Trials Unit of ITM who supported th...
10.4269/ajtmh.15-0890
article
en
2,016
false
false
true
false
Medicine
https://openalex.org/fields/27
cc-by
1%) members of which are capable to also degrade petroleum hydrocarbons 29. In addition to Desulfonauticus mentioned above, we identified in moderate amounts some OTUs that are related to anaerobic mesophilic sulfate and/or sulfur reducers such as Desulfovibrio (0. 8%), order Desulfovibrionales (2. 9%), Pelobacter (0....
10.1038/srep22960
article
en
2,016
true
false
false
false
Environmental Science
https://openalex.org/fields/23
cc-by
As suggested in Head et al. 45 , a cryptic community using oxygen, possibly provided by water radiolysis may be present in some oil reservoirs. Despite, the in situ role of recovered aerobic microbes and especially the hyperhalophilic archaeons in the Rabi core is questionable; it is known that some of them are able t...
10.1038/srep22960
article
en
2,016
true
false
false
false
Environmental Science
https://openalex.org/fields/23
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Dna extraction. A portion of the sediment was washed with the aim to remove hydrocarbons and PCR inhibitory material and improve DNA recovery and efficiency of PCR amplification 62. Washing was carried out by re-suspension in wash solution (2 mL per g of sample), vortexing for 2 min, followed by centrifugation at full...
10.1038/srep22960
article
en
2,016
true
false
false
false
Environmental Science
https://openalex.org/fields/23
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Sequence analyses. Sequence manipulations, analyses and alignments were performed using the BioEdit program (http://www. mbio. ncsu. edu/BioEdit/bioedit. html). Similarities of concensus 16S rRNA gene nucleotide sequences with sequences available in the Genbank database were determined using the BLASTN program (www3. n...
10.1038/srep22960
article
en
2,016
true
true
false
false
Environmental Science
https://openalex.org/fields/23
cc-by
8. 0 package 64. OTUs were picked using the uclast method 65 at the 97% threshold. Taxonomic assignments were performed against the Greengenes database (May 2013 version).
10.1038/srep22960
article
en
2,016
true
false
false
false
Environmental Science
https://openalex.org/fields/23
cc-by
Microscopic and SEM observations of the core did not show any physical evidence (disruption, mineralogical contamination) of penetration of the drilling fluid into the core. As the clays from core samples were green and not oxidized, it seems that coring processes did not affect physico-chemical conditions of the reser...
10.1038/srep22960
article
en
2,016
true
false
false
false
Environmental Science
https://openalex.org/fields/23
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Characterization Electron Microscopy TEM was performed by depositing 10 µL of the colloidal suspension on a carbon coated copper grid which is left to to dry in air for a few hours. The nanoparticles were imaged with a Tecnai G2 FEI microscope operated at 120kV. AgNWs, as well as the oriented monolayer thin films were ...
10.1039/c6fd00017g
article
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2,016
false
true
false
false
Materials Science
https://openalex.org/fields/25
cc-by
Acknowledgements The authors thank Marc Schmutz and Christian Blanck ( ICS, France ) for TEM measurements, Cedric Leuvrey ( IPCMS, France ) for SEM measurements. The sheet resistance measurements were done in the STnano cleanroom facility in Strasbourg, France. S. S. thanks the IDEX program of the Strasbourg U...
10.1039/c6fd00017g
article
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2,016
false
false
true
false
Materials Science
https://openalex.org/fields/25
cc-by
Acknowledgements We acknowledge financial support from CONICET ( PIP 291 ), PICT "MeMO" ( 0788 ) and CIC-Buenos Aires. We thank Dr. D. Vega , from the Laboratory of X-ray Diffraction ( GIA, GAIyANN, CAC, CNEA ), for the XRD measurements.
10.1016/j.physleta.2016.06.023
article
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2,016
false
false
true
false
Engineering
https://openalex.org/fields/22
cc-by
However, the SIRENE file did not supply numbers of employees but only workforces by size category, meaning on the one hand that no non-salaried employees were included in the file (it should be noted that 47% of establishments had no employee in 1994), and, on the other, that the number of salaried employees was known ...
10.1016/j.trpro.2016.02.057
article
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2,016
false
true
false
false
Engineering
https://openalex.org/fields/22
cc-by
Size effects due to differences in BSA were removed. Results were visualised by deforming the computed mean LV shape along the shape vector from low values for the control group towards high values for the ASO group. Differences were assessed using non-parametric Mann-Whitney-U test. Correlations between shape features...
10.1186/1532-429x-18-s1-q2
article
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2,016
true
false
false
false
Medicine
https://openalex.org/fields/27
cc-by
Acknowledgements This work was supported principally by the Michelin ® International Corporation and partly by the National Science Foundation award CMMI-1538002. We acknowledge several useful discussions with Dr. C. Khripin and Mr. Tianshu Liu.
10.1038/srep26867
article
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2,016
false
false
true
false
Engineering
https://openalex.org/fields/22
cc-by
Acknowledgements We thank the ESRF (Grenoble) for beamtime allocation at beamline ID26 in the frame of proposal MA 1530 and the nancial support.
10.1039/c5ra26302f
article
en
2,016
false
false
true
false
Engineering
https://openalex.org/fields/22
cc-by
These studies contain useful information about response time. Another challenge is the multi-objective optimization of mechatronic systems. Two techniques are currently used, the first is by aggregating all the criteria in one multi-criteria indicator [8]. The second method is to keep the criteria independent and try ...
10.1109/mecatronics.2016.7547164
preprint
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2,016
false
true
false
false
Computer Science
https://openalex.org/fields/17
cc-by
B. Modelica model Several multi-domain modeling tools as Bond-Graphs, Matlab/Simulink and Modelica are used for preliminary design of a mechatronic system [10]. If we use a software based on transfer functions we will lose the link with the physical parameters. And one of our objectives is to consider the physical tec...
10.1109/mecatronics.2016.7547164
preprint
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2,016
false
true
false
false
Computer Science
https://openalex.org/fields/17
cc-by
A. Problem description We are searching for the system configuration that respects the following specifications:  The rise time (0° to 90°) should be lower than 150 ms (12V supply voltage)  The return time with the failsafe system (90° to 10°) should be lower than 300ms (0V supply voltage and open circuit)  The maxi...
10.1109/mecatronics.2016.7547164
preprint
en
2,016
false
true
false
false
Computer Science
https://openalex.org/fields/17
cc-by
B. Results To carry out the optimization, Modelica was combined with ModelCenter software. And from the algorithms available in its library, we have chosen the NSGA II algorithm (Nondominating Sorting Genetic Algorithm) [11]. Since the problem is multi-objective, the solution is not unique but we obtain a set of Paret...
10.1109/mecatronics.2016.7547164
preprint
en
2,016
false
true
false
false
Computer Science
https://openalex.org/fields/17
cc-by
V. CONCLUSION This article has presented a novel approach of preliminary design and optimization of a mechatronic system. This approach was applied successfully to an Electronic Throttle. We first integrated the technology of the system in the preliminary design thanks to Modelica language. Then, a multiobjective optim...
10.1109/mecatronics.2016.7547164
preprint
en
2,016
false
true
false
false
Computer Science
https://openalex.org/fields/17
cc-by
ACKNOWLEDGMENT The presented work has been supported by the research project AAP FUI 19 -MIMe. The author would like to thank the members of this project who contributed to this work by their suggestions and help.
10.1109/mecatronics.2016.7547164
preprint
en
2,016
false
false
true
false
Computer Science
https://openalex.org/fields/17
cc-by
For example, Wang and Yu [15] used the semantic description of colours to associate an emotional semantic to an image. Concerning textures, the orientation of the different lines contained in the images is sometimes considered. According to Liu et al. [1] , oblique lines could be associated with dynamism and action; ...
10.1016/j.jvcir.2016.03.009
article
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2,016
true
false
false
false
Computer Science
https://openalex.org/fields/17