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cc-by | CONCLUSIONS Our analysis of the FIDELIS project data in China provides limited evidence that the project might have had a role in contributing to the increase in rates of detection of new sputum smear-positive cases of TB in certain project areas in China. In future these types of projects should perhaps be designed in... | 10.5588/pha.16.0007 | article | es | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Acknowledgments This study was supported by Ipsen Biopharmaceuticals, Inc. , Basking Ridge , NJ. S. Tanik, PhD , of ClinicalThinking, Hamilton, NJ and Debika Chatterjea, PhD , of MedVal Scientific Information Services, Skillman, NJ provided medical writing assistance which was funded by Ipsen. | 10.1007/s12029-016-9866-9 | review | en | 2,016 | false | false | true | false | Medicine | https://openalex.org/fields/27 |
cc-by | Acknowledgments This study was supported by Ipsen Biopharmaceuticals, Inc. , Basking Ridge , NJ. S. Tanik, PhD , of ClinicalThinking, Hamilton, NJ and Debika Chatterjea, PhD , of MedVal Scientific Information Services, Skillman, NJ provided medical writing assistance which was funded by Ipsen. | 10.1007/s12029-016-9866-9 | review | en | 2,016 | false | false | true | false | Medicine | https://openalex.org/fields/27 |
cc-by | Calves were separated from their dams immediately after birth, so that no suckling occurred. If a calf was suspected to have suckled, the dam data was removed from the final analysis. Cows were milked within the first 2 h post-calving. All information on calves at birth (date and time of birth, sex, weight, health stat... | 10.1017/s1751731115001962 | article | en | 2,015 | true | false | false | false | Veterinary | https://openalex.org/fields/34 |
cc-by | Colostral IgG1 concentration was determined via a semi-automated single radial immunodiffusion technique (Mancini et al. , 1965; Levieux and Ollier, 1999) using commercial kits (ID Ring BOV IgG; ID Biotech, Issoire, France). Sample dilution was adjusted to meet the manufacturer's recommendations (1000 times). Between... | 10.1017/s1751731115001962 | article | en | 2,015 | false | true | false | false | Veterinary | https://openalex.org/fields/34 |
cc-by | Distributions of continuous variables were examined and, if necessary, log-transformed (SCC to somatic cell score (SCS), but only SCC data are reported and discussed). To study IgG1 concentration and its average variation between quarters, ANOVA model was used including the effect of quarter and cow. In this approach, ... | 10.1017/s1751731115001962 | article | en | 2,015 | true | false | false | false | Veterinary | https://openalex.org/fields/34 |
cc-by | Acknowledgments The authors thank the technical staff at the Dairy Research Station at Méjusseaume , Le Rheu for taking care of the animals and running the colostrum and serum samplings. They also thank C. Doré for running the laboratory analysis, M. Kloareg for running the statistical analysis and A-T-T Scienti... | 10.1017/s1751731115001962 | article | en | 2,015 | false | false | true | false | Veterinary | https://openalex.org/fields/34 |
cc-by | ACKNOWLEDGMENTS The authors are supported by the French Ligue contre le Cancer (équipe labellisée); Agence National de la Recherche (ANR) -Projets blancs ; ANR under the frame of E-Rare-2, the ERA-Net for Research on Rare Diseases; Association pour la recherche sur le cancer (ARC) ; Cancéropôle Ile-de-France ; ... | 10.18632/oncotarget.13892 | article | fr | 2,016 | false | false | true | false | Medicine | https://openalex.org/fields/27 |
cc-by | Background: Most estimates of postpartum hemorrhage (PPH) are calculated from studies that use administrative or medical birth databases, and only a few from prospective observational studies. Our principal objective was to estimate the incidence of PPH according to their severity (mild or severe) in vaginal deliveries... | 10.1186/s12884-016-1008-7 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | The prevalence of PPH (defined as ≥500 mL blood loss) and severe PPH (defined as ≥1 000 mL blood loss) are reported to be around 6. 0 and 1. 86 % of all deliveries, respectively, and vary widely throughout the world [23]. Most estimates are calculated from observational studies that use administrative or medical birth... | 10.1186/s12884-016-1008-7 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Data source We contacted 43 French perinatal networks to request their support for this study of their maternity units. Twenty perinatal networks, covering 231 eligible maternity units, agreed to support this 6-month project (1 February 2011 to 31 July 2011). Finally, 182 maternity units participated, for a participati... | 10.1186/s12884-016-1008-7 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Blood loss was to be estimated visually with the habitual method used in each unit and specified in each woman's records. Professionals in each unit collected data prospectively for 6 months, entering it onto electronic case report forms via a secure website. The principal endpoint was the incidence of PPH. Quality con... | 10.1186/s12884-016-1008-7 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Oxytocin is the agent used most frequently, even after cesareans. The percentage of active management of the third stage of labor has increased in France since 2006 [45]. It is probable that the national rate in 2015 is still better, after the 2014 publication of the updated French guidelines. Our results are consiste... | 10.1186/s12884-016-1008-7 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Acknowledgements ' HERA GROUP : Bernard Branger, MD , is a pediatrician; Laure Connan, MD, is an obstetrician-gynecologist; Catherine Crenn-Hébert, MD, is an obstetriciangynecologist; Caroline Da Costa Correia is a midwife; Michel Dreyfus, MD, PhD , is a professor in obstetrics and gynecology; Paul Lefèvre, MD , i... | 10.1186/s12884-016-1008-7 | article | en | 2,016 | false | false | true | false | Medicine | https://openalex.org/fields/27 |
cc-by | Availability of date and material The datasets generated and analyzed during the current study are not publicly available because they belong to the institutional promotor of the Hera study (Centre Hospitalier Universitaire de Clermont-Ferrand), but are available from the corresponding author on reasonable request. | 10.1186/s12884-016-1008-7 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Statistical analysis The different analyses were performed using the StatView 1 software. Qualitative variables were reported as absolute and relative frequencies (expressed in %) and compared using Fisher's exact test. Quantitative variables were described by mean and standard deviation and median and interquartile ra... | 10.1371/journal.pone.0156593 | article | en | 2,016 | false | true | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Acknowledgements We thank Ms Jane Stock for preparing summaries of meeting discussions. Ciber Fisiopatologı ´a de la Obesidad y Nutricion is an initiative of Instituto de Salud Carlos III, Spain. | 10.1373/clinchem.2016.258897 | article | en | 2,016 | false | false | true | false | Medicine | https://openalex.org/fields/27 |
cc-by | Methods Isolate details. Six MTBC isolates were cultivated from sputum samples collected from newly diagnosed pulmonary TB (PTB) patients attending selected health centres and hospitals in various districts of Amhara Region, Ethiopia. The isolates were part of 237 Mtb isolates collected between 2008 and 2010 for the TB... | 10.1099/mgen.0.000063 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | , 2013). The International Spoligotype Database (SpolDB4, 2011) was used to assign the spoligotyping patterns to major genetic lineages and sub-lineages (Brudey et al. , 2006). New spoligo patterns that were not described by SpolDB4 were assigned to Mtb families by SpolDB3 and a randomly initialized model (Run SPOT-CL... | 10.1099/mgen.0.000063 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Sequencing, assembly and variant calling. Among the six isolates transferred to RIVM for whole-genome sequencing, one (Solo 80) could not be subcultured despite repeated attempts. DNA was thus extracted from five isolates using standard methods. Whole-genome sequencing was successfully performed by BaseClear BV (Leiden... | 10.1099/mgen.0.000063 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | 10. 0. The quality of the FASTQ sequences was enhanced by trimming off low-quality bases using the 'Trim sequences' option of the CLC Genomics Workbench version 6. 5. The quality-filtered FASTQ sequences were assembled using Ray, and the resulting contigs were mapped to both Mycobacterium tuberculosis H37Rv (GenBank ac... | 10.1099/mgen.0.000063 | article | en | 2,016 | true | true | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Phylogenetic tree reconstruction and comparison of sequences. A parsimony-based phylogeny (Phylip, version 3. 695) of the MTBC was reconstructed using 78 613 concatenated variable positions (SNPs) that were found in at least one of 100 previously characterized RIVM strains of different lineages and the five strains fro... | 10.1099/mgen.0.000063 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Discussion Mycobacterium tuberculosis, a member of the MTBC, has been a significant cause of morbidity and mortality among humans for thousands of years. Phylogenetic studies have shown that the MTBC comprises at least seven distinct lineages distributed in different geographical locations around the world. Current evi... | 10.1099/mgen.0.000063 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | (2013). The inclusion of the phylogenetically most distant M. canettii strain STB-K (CIPT 1400700100) (Supply et al. , 2013; Blouin et al. , 2014; Boritsch et al. , 2016) as the outgroup has reinforced the clusters observed among the different Mtb. We found four newly identified deletions characteristic of all five l... | 10.1099/mgen.0.000063 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Acknowledgements This work was supported by the Research Council of Norway, GLOBVAC programme (grant no. 192468/S50 ). R. B. acknowledges support from the Fondation pour la Recherche Médicale (FRM; grant no. DEQ20130326471 ). | 10.1099/mgen.0.000063 | article | en | 2,016 | false | false | true | false | Medicine | https://openalex.org/fields/27 |
cc-by | Data Summary 1. Reads and assembled scaffolds have been deposited in the European Nucleotide Archive (study accession number PRJEB8432; http://www. ebi. ac. uk/ena/data/ view/PRJEB8432). | 10.1099/mgen.0.000063 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Data Summary 1. Reads and assembled scaffolds have been deposited in the European Nucleotide Archive (study accession number PRJEB8432; http://www. ebi. ac. uk/ena/data/ view/PRJEB8432). | 10.1099/mgen.0.000063 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Panobinostat is an oral pan-histone deacetylase inhibitor developed by Novartis. Panobinostat acts via epigenetic modification and inhibition of the aggresome pathway. In August 2015, the European Commission authorized panobinostat for use in combination with bortezomib and dexamethasone for the treatment of relapsed o... | 10.1007/s12325-016-0413-7 | review | en | 2,016 | false | false | false | true | Medicine | https://openalex.org/fields/27 |
cc-by | Bortezomib Bortezomib was the first PI to be authorized for the treatment of MM by the EC (Table 1 ). Bortezomib is a boronic acid analog that reversibly binds and inhibits the chymotryptic activity of the 20S proteasome subunit [14]. In the phase 3 APEX trial (NCT00048230), bortezomib was superior to dexamethasone f... | 10.1007/s12325-016-0413-7 | review | en | 2,016 | false | false | false | true | Medicine | https://openalex.org/fields/27 |
cc-by | One study showed that a DACi (panobinostat) reactivated genes repressed by the hypermethylation of H3K27 by the Polycomb group proteins and reduced proliferation and increased apoptosis in human MM cell lines [64]. Additionally, panobinostat led to gene upregulation, reduction of tumor load, and increased OS in preclin... | 10.1007/s12325-016-0413-7 | review | en | 2,016 | false | false | false | true | Medicine | https://openalex.org/fields/27 |
cc-by | 0001 NR vs 7. 3 P\0. 0001 BTZ bortezomib, CFZ carfilzomib, DARA daratumumab, Dex dexamethasone, ELO elotuzumab, NA not available, NR MM multiple myeloma, LEN lenalidomide, not reached, TTP time to treatment POM pomalidomide, ORR overall response rate, OS overall survival, PAN panobinostat, PFS progression-free survival... | 10.1007/s12325-016-0413-7 | review | en | 2,016 | false | false | false | true | Medicine | https://openalex.org/fields/27 |
cc-by | However, in the phase 3 ELOQUENT-2 trial (NCT01239797), the addition of elotuzumab to the backbone of lenalidomide and dexamethasone in patients with 1-3 prior therapies led to a prolonged median PFS [19. 4 vs 14. 9 months; HR, 0. 70 (95% CI, 0. 57-0. 85); P\0. 001] and increased ORR (79% vs 66%; P\0. 001) compared wit... | 10.1007/s12325-016-0413-7 | review | en | 2,016 | false | false | false | true | Medicine | https://openalex.org/fields/27 |
cc-by | 5 months (Table 2) [46]. In the dose-escalation phase 1/2 study GEN 501 (NCT00574288), daratumumab demonstrated clinical efficacy in patients with RRMM who were refractory to C2 prior lines of therapy. In the dose-expansion part of the study, patients received 8 mg/kg (n = 30) or 16 mg/kg (n = 42) of daratumumab. The O... | 10.1007/s12325-016-0413-7 | review | en | 2,016 | false | false | false | true | Medicine | https://openalex.org/fields/27 |
cc-by | ACKNOWLEDGMENTS No funding or sponsorship was received for this study, publication of this article or the versus high-dose dexamethasone alone for patients with relapsed and refractory multiple myeloma (MM-003): a randomised, open-label, phase 3 trial. Lancet Oncol. 2013;14:1055-66. 32. Dimopoulos MA, Weisel KC, Cavo M... | 10.1007/s12325-016-0413-7 | review | en | 2,016 | false | false | true | false | Medicine | https://openalex.org/fields/27 |
cc-by | Patients Patients were followed in the French Reference Center for Lysosomal Diseases. A total of 35 type 1 GD patients were recruited prospectively between June 2012 and July 2015. Patients were considered anemic when the hemoglobin (Hb) levels were strictly below 11. 5 g/dL for children between 2 and 12 years old, 12... | 10.3324/haematol.2016.147546 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | GD erythroid progenitors exhibit decreased proliferative capacity Cell expansion and viability were measured during the two culture phases using trypan blue staining. The cell proliferation capacity is reported as the absolute number of viable cells at days 0 and 15 of culture. Our results showed a decrease of the prol... | 10.3324/haematol.2016.147546 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Acknowledgments The authors thank Monia Bengherbia and Karima Yousfi for their assistance in collecting GD patient data, Emmanuel Collec for his contribution to the collection of patient samples and Geneviève Courtois and Cyril Mignot for their helpful discussions and advice. | 10.3324/haematol.2016.147546 | article | en | 2,016 | false | false | true | false | Medicine | https://openalex.org/fields/27 |
cc-by | Funding This study was supported by a grant from Shire. N. R. was supported by a Labex GR-Ex fellowship. The Labex GR-Ex , reference ANR-11-LABX-0051 , is funded by the " Investissements d'avenir" program of the French National Research Agency , reference ANR-11-IDEX-0005-02. | 10.3324/haematol.2016.147546 | article | en | 2,016 | false | false | true | false | Medicine | https://openalex.org/fields/27 |
cc-by | Funding This study was supported by a grant from Shire. N. R. was supported by a Labex GR-Ex fellowship. The Labex GR-Ex , reference ANR-11-LABX-0051 , is funded by the " Investissements d'avenir" program of the French National Research Agency , reference ANR-11-IDEX-0005-02. | 10.3324/haematol.2016.147546 | article | en | 2,016 | false | false | true | false | Medicine | https://openalex.org/fields/27 |
cc-by | For each SNP bin (r 2 > 0. 5), SNP with the most significant evidence for association was included in the multivariate model. Multivariate analyses were done by stepwise conditional logistic regression (SAS v. 9. 3). Logistic regression was also used to estimate the odds ratio for each individual SNP in the T1R-affecte... | 10.1371/journal.pntd.0004412 | article | en | 2,016 | false | true | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Parkinson's disease and inflammatory bowel disease data Genotype associations of LRRK2 and PD were obtained from the PDgene database (www. pdgene. org). LRRK2 variants associated with PD were obtained from a GWAS meta-analysis of 14 studies with a total population sample of 12,771 PD cases and 93,386 controls [32]. Ge... | 10.1371/journal.pntd.0004412 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Acknowledgments We thank all leprosy patients and their family who participated in this study. We thank all CLINT ( Canadian LRRK2 in Inflammation Team ) researchers, the members of the Schurr lab and the members of the laboratory for Human Genetics of Infectious Diseases in Paris and New York City for useful discu... | 10.1371/journal.pntd.0004412 | article | en | 2,016 | false | false | true | false | Medicine | https://openalex.org/fields/27 |
cc-by | 5-conjugated anti-DC-SIGN (DC-specific ICAM-3 grabbing-nonintegrin or CD209, member of the CLR family, specific marker of immature DCs), Alexa Fluor Ý 488-conjugated anti-MR (Mannose Receptor or CD206, member of the CLR family, specific marker of immature DCs and macrophages), and streptavidin APC-conjugated anti-TLR4 ... | 10.1155/2016/9747480 | article | en | 2,016 | false | true | false | false | Immunology and Microbiology | https://openalex.org/fields/24 |
cc-by | Flow Cytometric Analysis of Microbial Effects on DC Phenotype. During the immune response, bacterium internalization can then induce DC activation and maturation, resulting in modifications detectable by cytometry of numerous membrane markers and permitting to distinguish immature DCs from mature ones. In our study, DC... | 10.1155/2016/9747480 | article | en | 2,016 | true | false | false | false | Immunology and Microbiology | https://openalex.org/fields/24 |
cc-by | Acknowledgments The authors thank the CICS of Clermont-Ferrand and are very grateful to Christelle Blavignac , Claire Szczepaniak , and. | 10.1155/2016/9747480 | article | en | 2,016 | false | false | true | false | Immunology and Microbiology | https://openalex.org/fields/24 |
cc-by | Acknowledgments The authors thank the CICS of Clermont-Ferrand and are very grateful to Christelle Blavignac , Claire Szczepaniak , and. | 10.1155/2016/9747480 | article | en | 2,016 | false | false | true | false | Immunology and Microbiology | https://openalex.org/fields/24 |
cc-by | Source of data This was a prospective observational cohort study using data from the Pharmacoepidemiologic General Research eXtension (PGRx) information system which is a set of population-based registries including case-patients (cases) with specific diseases recruited by their specialist physician and referent-patien... | 10.3324/haematol.2016.146373 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Measures All patient data were collected through dedicated case report forms (CRFs), similar in cases and controls regarding socio-demographics, personal medical history and family history of autoimmune disorder (in first-degree relatives): multiple sclerosis, lupus erythematosus, rheumatoid arthritis, Crohn disease, c... | 10.3324/haematol.2016.146373 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Statistical analysis Statistical analysis was performed with SAS software version 9. 18 and all comparative tests were two-sided with a type-1 error set at α=0. 05. Bleeding symptoms were categorized into: a) no bleeding; b) cutaneous bleeding alone; and c) severe bleeding defined as mucocutaneous and/or visceral bleed... | 10.3324/haematol.2016.146373 | article | en | 2,016 | false | true | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Ethics approval This research was approved by the consultative committee for non-interventional research (Comité Consultatif sur le Traitement de l'Information en Matière de Recherche) and the French Data Protection Authority (Commission Nationale de l'Informatique et des Libertés) was informed that data were being col... | 10.3324/haematol.2016.146373 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Cytoplasmic localization of RAP1 has also been reported, as well as its involvement in NF-B signalling (35, 36). A recent study shows that RAP1 can affect TRF2 interaction properties toward duplex DNA (37). In TeloPIN database (5) , RAP1 and TRF2 interact respectively with 131 and 247 partners, among which 32 are co... | 10.1093/nar/gkv1531 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | HJ protection A total of 5 nM of telomeric HJ (tHJ) labelled on one strand was incubated with different concentrations of proteins in 10 l of binding buffer containing 10 mM MgAc 2 and digested for 60 min using 2 nM of T7 Endonuclease I, MBP-CceI and MBP-RusA or 50 nM of GEN1 1 -527. Reactions were performed at 4 • C f... | 10.1093/nar/gkv1531 | article | en | 2,016 | false | true | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Raw scanning force microscopy images were flattened using the manufacturer's software and converted into TIF files. Contour lengths (CLs) were measured by the read-through length method using SigmaScan Pro software (SPSS Inc, Chicago, IL, USA). Volumes calculated as half-ellipsoids as published (38). Between 150 and 2... | 10.1093/nar/gkv1531 | article | en | 2,016 | true | true | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | SAXS SAXS experiments were performed at SWING beamline, synchrotron SOLEIL. TRF2, RAP1 and RAP1-RCT were set in the buffer containing 20 mM Tris pH = 7. 5, 150 mM NaCl, 5 mM 2-mercaptoethanol and 0. 2 mM PMSF. TRF2 was concentrated to 215 M (monomers), RAP1 to 326. 7 M, RAP1-RCT to 1015. 7M, TRF2 and RAP1 were mixed to... | 10.1093/nar/gkv1531 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Crystallization and structure determination Crystals of TRFH in complex with peptide RAP1-YLP 89 ENRERLELEAYRLGPASA 106 were obtained by hanging drop vapour diffusion at room temperature, with a concentration of TRFH of 34 mg/ml and a molar ratio of TRFH/Rap1-YLP of 1:3. 5 in a buffer containing 12. 5 mM Tris pH 8. 0, ... | 10.1093/nar/gkv1531 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | SEC-MALS TRF2 protein at a concentration of 100 M (in a buffer containing 50 mM Tris, pH 7. 5, 150 mM NaCl, 10 mM 2-mercaptoethanol) was applied to Bio SEC-3 column (Agilent) on a HPLC Shimadzu system coupled to MALS/QELS/UV/RI (Wyatt Technology). Data were analysed using ASTRA software after calibration with a bovine ... | 10.1093/nar/gkv1531 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Model building and conservation analysis The BRCT domain of RAP1 was modelled using MOD-ELLER software (44) and the structure of the BRCT domain of Rap1 from S. cerevisiae (45, PDB entry 2L42). The resulting model and Myb domain of RAP1 (46) were then manually positioned along the TRFH domain of TRF2 with Pymol (47... | 10.1093/nar/gkv1531 | article | en | 2,016 | true | true | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | In order to delineate the structural determinants of this interaction we solved the crystal structure of the RAP1-YLP/TRFH complex at 2. 4 Å resolution (Figure 3A and B ). The superimposition of our structure with this of TRFH in complex with the Apollo-YLP peptide (PDB entry: 3BUA, 15) shows that the structures are ... | 10.1093/nar/gkv1531 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Looking from the top of the TRFH domain, we observe that this interaction of the N-terminal region of RAP1 induces an unexpected dimerization of the BRCT domain (Figure 4C , right). The high conservation among vertebrates of the known YXLXP binding site of TRF2, of the YRLXP motif of RAP1 and of the surface of RAP1 in... | 10.1093/nar/gkv1531 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | (A) TRFH/RAP1-YLP peptide crystal structure; (B) Juxtaposition of RAP1[1-121] 3D model calculated with Modeller (41), and RAP1-Myb nuclear magnetic resonance structure (PDB entry: 1FEX). (C) Positioning of RAP1 BRCT and Myb domains on TRFH surface based on combination of the crystal structure and footprinting experimen... | 10.1093/nar/gkv1531 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | ACKNOWLEDGEMENTS The authors wish to thank Stephen CY Ip , Stephen C West , Anne-Cécile Déclais and David MJ Lilley for kindly providing resolving enzymes or plasmids to produce them. We thank Dr Carl Mann for his help with cell culture and for the HeLa cells he provided, Magali Aumont-Nicaise for her support... | 10.1093/nar/gkv1531 | article | en | 2,016 | false | false | true | false | Medicine | https://openalex.org/fields/27 |
cc-by | Author contributions: G. G. , S. M. , S. P. , Y-VLB, R. T. , M-. J. G-. P. , E. G. and M-. H. L. D. conceived and designed the experiments. G. G. , S. M. , S. P. , R. B. , Y-. V. L. B. , C. T. L. , W. M. , P. R. , B. G. , R. T. , M-. J. G-. P. and M-. H. L. D. performed the experiments. G. G. , S. M. , S. P. , R. B. , ... | 10.1093/nar/gkv1531 | article | en | 2,016 | false | false | true | false | Medicine | https://openalex.org/fields/27 |
cc-by | (A) TRFH/RAP1-YLP peptide crystal structure; (B) Juxtaposition of RAP1[1-121] 3D model calculated with Modeller (41), and RAP1-Myb nuclear magnetic resonance structure (PDB entry: 1FEX). (C) Positioning of RAP1 BRCT and Myb domains on TRFH surface based on combination of the crystal structure and footprinting experimen... | 10.1093/nar/gkv1531 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Sources of data All laboratory-confirmed human cases of avian influenza A(H7N9) virus infection were notified to the Chinese Center for Disease Control and Prevention (China CDC) and relevant clinical and epidemiological data was recorded in an electronic database [2, 3]. Data extracted for this study included age, se... | 10.1371/journal.pone.0148506 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | In this analysis and the analyses described below, we specified flat priors for each parameter, and drew 10,000 samples from the posterior distributions after a burn-in of 5,000 iterations. Analyses were conducted using R version 3. 2. 2 (R Foundation for Statistical Computing, Vienna, Austria). The raw data and R synt... | 10.1371/journal.pone.0148506 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Results There were 438 confirmed H7N9 cases included in the China CDC database as of the 5th August 2014, and 43 of these were recorded as "mild" cases and were excluded from the analyses here because they form only a small sample of mild H7N9 infections. Among the 395 patients included in this study who all required h... | 10.1371/journal.pone.0148506 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | 8-2. 0), which could reflect differences regarding pathogenesis between these different virus strains [20]. We also observed a significant increased risk of death among older patients. To assess the effect of this potential cofounding factor, we applied a stratified analysis using two age groups (0-59 years old and >6... | 10.1371/journal.pone.0148506 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Acknowledgments We thank staff members of the Bureau of Disease Control and Prevention and Health Emergency Response Office of the National Health and Family Planning Commission and provincial and local departments of health for providing assistance with administration and data collection; staff members at county-,... | 10.1371/journal.pone.0148506 | article | en | 2,016 | false | false | true | false | Medicine | https://openalex.org/fields/27 |
cc-by | The data can be located through the following link at the Dryad Digital Repository: http://dx. doi. org/10. 5061/dryad. g2j62. | 10.1371/journal.pone.0148506 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Acknowledgments This work was supported in part by grants from the French National Agency for Research called BInvestissements d' AvenirL abex IRON no. ANR-11-LABX-0018-01 and Equipex ArronaxPlus no. ANR-11-EQPX-0004. Open Access This article is distributed under the terms of the Creative Comm ons Attribution 4. 0... | 10.1007/s00259-016-3458-6 | editorial | en | 2,016 | false | false | true | false | Medicine | https://openalex.org/fields/27 |
cc-by | 47) and 48 (33-91; P = 0. 15) days among the MAC recipients. With a median follow-up of 5. 6 years, 41 relapses (30%) and 57 deaths (42%) were recorded. The cumulative incidence of relapse (CIR) at 10 years was 15% (95% CI 7-23) in the group of patients who experienced viral reactivations compared with 54% (95% CI 41-7... | 10.1097/md.0000000000005356 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Acknowledgments The authors thank all the members of the G. A. E. L ( Gaël Adolescent Espoir Leucémie ) and A. G. M. O. M. P ( Association des Greffés de Moelle Osseuse de Midi-Pyrénées ) associations for their kind support to patients. They would also like to thank the data management unit of Toulouse University Ho... | 10.1097/md.0000000000005356 | article | en | 2,016 | false | false | true | false | Medicine | https://openalex.org/fields/27 |
cc-by | interpreted data, and wrote the manuscript. Funding: CR has received commercial research grants from Amgen , Celgene , and Chugai and is a consultant/advisory board member of Celgene and Sunesis Laboratories. This work has been granted by granted by the French government under the " Investissement d'avenir" pr... | 10.1097/md.0000000000005356 | article | en | 2,016 | false | false | true | false | Medicine | https://openalex.org/fields/27 |
cc-by | Study type In 2013, a study using data from all the cases of dengue seen in Cayenne hospital was collected. The patients were seen between 1 and 15 times between their first consultation and the end of the illness thus the study was longitudinal and allowed the calculation of incidence rates and survival analysis. The ... | 10.1371/journal.pone.0150828 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | For Cayenne alone, there were an estimated 7231 cases, with 2672 probable or confirmed cases, 340 of whom were hospitalized (4. 70%) 46 being severe (0. 64%), with 2 deaths(2. 7 per 10 000). The hospitalization rate was thus the highest to date. Patients that were not hospitalized were followed up every day at the infe... | 10.1371/journal.pone.0150828 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Data analysis Data analysis was performed using Stata 13. Quantitative variables were categorized according to statistical criteria using the 10th and 90th percentiles, the first and third quartiles and the median. The failure event was the first central nervous system dysfunction reported (defined as decreased Glasgow... | 10.1371/journal.pone.0150828 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Discussion Here in a small city with easy access to emergency care, quality care and a policy of hospitalizing patients with any clinical, biological or social warning sign, over 20% of the patients seen for dengue fever had central nervous system dysfunction mostly around the 5th or 6th day of fever. This proportion i... | 10.1371/journal.pone.0150828 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | This study was supported by the grant " Investissement d'Avenir " from the Agence Nationale de la Recherche (CEBA reference no. ANR-10-LABX-0025 ) which provides yearly funding to the EA3593 laboratory. CI = 1. | 10.1371/journal.pone.0150828 | article | en | 2,016 | false | false | true | false | Medicine | https://openalex.org/fields/27 |
cc-by | Genome Sequencing, Assembly and Annotation The genome of BrMV was extracted using the automated EZ1 Virus Mini-Kit v. 2 (Qiagen GmbH, Hilden, Germany) according to the manufacturer's instructions. DNA quality and concentration were checked using a nanodrop spectrophotometer (Thermo Scientific, Waltham, MA, USA). Sequen... | 10.3390/v8030076 | article | en | 2,016 | true | true | false | false | Environmental Science | https://openalex.org/fields/23 |
cc-by | Finally, the genome annotation was manually revised and curated. The predicted proteins that were smaller than 100 amino acids and had no hit in any database were ruled out. Those larger than 100 amino acids without hit in any database (so-called ORFans) were kept and analyzed using the PSI-BLAST tool to detect distant... | 10.3390/v8030076 | article | en | 2,016 | true | false | false | false | Environmental Science | https://openalex.org/fields/23 |
cc-by | Comparative Genomic and Pan-Genome Analysis The genome synteny between the BrMV and other marseilleviruses was checked using the MAUVE program [37]. The Proteinortho tool [38] was used to define the strict core of bona fide orthologs shared among BrMV and amoebal marseilleviruses from lineages A-C, using the recipro... | 10.3390/v8030076 | article | en | 2,016 | false | true | false | false | Environmental Science | https://openalex.org/fields/23 |
cc-by | To estimate the size of the pan-genome of the Marseilleviridae family, their predicted proteins was clustered using the BLASTclust program [42] using an amino acid sequence identity of 30% and sequence coverage of 50% as thresholds. We also described pan-genome size variation by stepwise inclusion of each new virus a... | 10.3390/v8030076 | article | en | 2,016 | false | true | false | false | Environmental Science | https://openalex.org/fields/23 |
cc-by | Phylogeny We performed a hierarchical-clustering based on the gene presence/absence pattern of 5443 NCVOGs, using the MeV tool [43] with Pearson correlation as distance metric. The phylogenetic tree was visualized using the FigTree v1. 4. 1 tool [44]. In addition, the five Megavirales core genes, namely the family B... | 10.3390/v8030076 | article | en | 2,016 | false | true | false | false | Environmental Science | https://openalex.org/fields/23 |
cc-by | Brazilian Marseillevirus Genome and Annotation The BrMV genome (GenBank accession No. : KT752522) is a circular, double-stranded DNA molecule composed of 362,276 bp (Supplementary Figure S2 ). This is compatible with the genome sizes of the other marseilleviruses, which range from 346,754 bp (Lausannevirus; NC_015326.... | 10.3390/v8030076 | article | en | 2,016 | true | false | false | false | Environmental Science | https://openalex.org/fields/23 |
cc-by | Viruses 2016, 8, 76 5 of 16 A total of 446/491 ORFs (90. 8%) from the BrMV had significant BLASTp matches (coverage ě 50%; similarity ě 50%; e-value ď 10 ´6) to marseillevirus protein sequences available in the NCBI nr database, with a mean identity of 77. 2%. The greatest number of best hits was with the Tunisvirus is... | 10.3390/v8030076 | article | en | 2,016 | true | false | false | false | Environmental Science | https://openalex.org/fields/23 |
cc-by | Moreover, only two ORFs (ORF364, a hypothetical protein; ORF381, a ubiquinone) predicted in the BrMV genome showed 100% identity with another marseillevirus, Lausannevirus (group B). A BLAST search against the NCBI nr database identified one sequence (ORF258-181 aa), with 50% coverage and 34% identity (e-value: 3 ˆ10 ´... | 10.3390/v8030076 | article | en | 2,016 | true | false | false | false | Environmental Science | https://openalex.org/fields/23 |
cc-by | 8% of hypothetical proteins (294 of the 491 predicted proteins). Table 1 describes the PSI-BLAST predictions for 23/29 ORFs tentatively classified as ORFans (79. 3%) and highlights the presence of atypical viral proteins such as ORF 337, which was predicted to encode a cytochrome C-like protein, previously described ... | 10.3390/v8030076 | article | en | 2,016 | false | true | false | false | Environmental Science | https://openalex.org/fields/23 |
cc-by | polyphaga but can replicate in A. castellanii. In contrast, replication of BrMV is restricted to the tested A. castellanii strains and no cytopathic effects or viral titers were detected in the A. polyphaga cells infected with this virus. When comparing the replication profile of BrMV and the Marseillevirus prototype i... | 10.3390/v8030076 | article | en | 2,016 | true | false | false | false | Environmental Science | https://openalex.org/fields/23 |
cc-by | The presence of such short pseudoORF may be a mere annotation artefact, or may represent novel short transcripts. We had identified 47 short ORFs encoding less than 100 amino acids with some correspondence in the NCBI nr database. It is worth mentioning that pseudoORF BrMV sequences were not identified in other marseil... | 10.3390/v8030076 | article | en | 2,016 | true | false | false | false | Environmental Science | https://openalex.org/fields/23 |
cc-by | polyphaga but can replicate in A. castellanii. In contrast, replication of BrMV is restricted to the tested A. castellanii strains and no cytopathic effects or viral titers were detected in the A. polyphaga cells infected with this virus. When comparing the replication profile of BrMV and the Marseillevirus prototype i... | 10.3390/v8030076 | article | en | 2,016 | true | false | false | false | Environmental Science | https://openalex.org/fields/23 |
cc-by | The presence of such short pseudoORF may be a mere annotation artefact, or may represent novel short transcripts. We had identified 47 short ORFs encoding less than 100 amino acids with some correspondence in the NCBI nr database. It is worth mentioning that pseudoORF BrMV sequences were not identified in other marseil... | 10.3390/v8030076 | article | en | 2,016 | true | false | false | false | Environmental Science | https://openalex.org/fields/23 |
cc-by | Acknowledgments: We would like to thank our colleagues from GEPVIG (Grupo de Estudo e Prospecção de Vírus Gigantes) and Laboratório de Vírus of Universidade Federal de Minas Gerais. In addition, CNPq ( Conselho Nacional de Desenvolvimento Científico e Tecnológico) , CAPES ( Coordenação de Aperfeiçoamento de Pess... | 10.3390/v8030076 | article | en | 2,016 | false | false | true | false | Environmental Science | https://openalex.org/fields/23 |
cc-by | Methods This is a phase III, multicenter, placebo-controlled, double-blind, randomized (1:1), two parallel arm clinical trial conducted in 17 public hospitals in Thailand (see Fig. 1 ). The list of locations is available at Clini-calTrials. gov Identifier NCT01745822. The primary objective is to assess the efficacy of ... | 10.1186/s12879-016-1734-5 | article | en | 2,016 | false | false | false | true | Medicine | https://openalex.org/fields/27 |
cc-by | Discussion This phase III randomized placebo-controlled doubleblind trial will assess the efficacy and safety of a short course antiviral treatment to prevent mother-to-child transmission of HBV. Enrollment was opened in January 2013 and accrual was completed on August 19, 2015 with no significant operational issues. T... | 10.1186/s12879-016-1734-5 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
cc-by | Acknowledgements We are grateful to Amy Sandul , Centers for Disease Control and Prevention , for careful and helpful editing of the manuscript. | 10.1186/s12879-016-1734-5 | article | en | 2,016 | false | false | true | false | Medicine | https://openalex.org/fields/27 |
cc-by | Acknowledgements We are grateful to Amy Sandul , Centers for Disease Control and Prevention , for careful and helpful editing of the manuscript. | 10.1186/s12879-016-1734-5 | article | en | 2,016 | false | false | true | false | Medicine | https://openalex.org/fields/27 |
cc-by | Flow cytometry and transcriptional analyses Cell suspensions were incubated for 40 min with cold PBS (Life Technologies) containing 10% FCS, FcR block (2. 4G2), a viability dye (eFluor 506; eBioscience #65-0866-14) and fluorochrome-bound Abs. To detect intracellular Ags, cells were fixed for 40 min with 2% paraformalde... | 10.4049/jimmunol.1502673 | article | en | 2,016 | true | false | false | false | Immunology and Microbiology | https://openalex.org/fields/24 |
cc-by | Statistical analysis Data were compiled and analyzed with GraphPad Prism software (version 6). Paired and unpaired Student t tests were used to determine statistical differences between groups. All graphs show mean + SEM. The p values , 0. 05 were considered significant. | 10.4049/jimmunol.1502673 | article | en | 2,016 | false | true | false | false | Immunology and Microbiology | https://openalex.org/fields/24 |
cc-by | Acknowledgments We thank Ana Cumano for the gift of OP9 stromal cells, Ofer Mandelboim for Ncr1 GFP mice, and Hans-Jorg Fehling for Rosa26 RFP mice. | 10.4049/jimmunol.1502673 | article | en | 2,016 | false | false | true | false | Immunology and Microbiology | https://openalex.org/fields/24 |
cc-by | Purpose: This retrospective review examined visual acuity (VA) in subjects with neovascular age-related macular degeneration and identified early and delayed response to ranibizumab. Procedures: MARINA, ANCHOR, HARBOR, and CATT published data were examined for response with monthly versus individualized dosing and pred... | 10.1159/000451065 | article | en | 2,016 | true | false | false | false | Medicine | https://openalex.org/fields/27 |
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