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Invasion of minor veins of tobacco leaves inoculated with tobacco mosaic virus mutants defective in phloem-dependent movement.
|
To fully understand vascular transport of plant viruses, the viral and host proteins, their structures and functions, and the specific vascular cells in which these factors function must be determined. We report here on the ability of various cDNA-derived coat protein (CP) mutants of tobacco mosaic virus (TMV) to invade vascular cells in minor veins of Nicotiana tabacum L. cv. Xanthi nn. The mutant viruses we studied, TMV CP-O, U1mCP15-17, and SNC015, respectively, encode a CP from a different tobamovirus (i.e., from odontoglossum ringspot virus) resulting in the formation of non-native capsids, a mutant CP that accumulates in aggregates but does not encapsidate the viral RNA, or no CP. TMV CP-O is impaired in phloem-dependent movement, whereas U1mCP15-17 and SNC015 do not accumulate by phloem-dependent movement. In developmentally-defined studies using immunocytochemical analyses we determined that all of these mutants invaded vascular parenchyma cells within minor veins in inoculated leaves. In addition, we determined that the CPs of TMV CP-O and U1mCP15-17 were present in companion (C) cells of minor veins in inoculated leaves, although more rarely than CP of wild-type virus. These results indicate that the movement of TMV into minor veins does not require the CP, and an encapsidation-competent CP is not required for, but may increase the efficiency of, movement into the conducting complex of the phloem (i.e., the C cell/sieve element complex). Also, a host factor(s) functions at or beyond the C cell/sieve element interface with other cells to allow efficient phloem-dependent accumulation of TMV CP-O.
|
['Capsid', 'Genes, Viral', 'Immunohistochemistry', 'Mutagenesis', 'Plant Diseases', 'Plants, Toxic', 'Tobacco', 'Tobacco Mosaic Virus', 'Viral Structural Proteins']
| 8,855,325
|
[['A21.249.500.250'], ['G05.360.340.024.340.364.875', 'G05.360.340.358.024.875', 'G05.360.340.358.840.500'], ['E01.370.225.500.607.512', 'E01.370.225.750.551.512', 'E05.200.500.607.512', 'E05.200.750.551.512', 'E05.478.583', 'H01.158.100.656.234.512', 'H01.158.201.344.512', 'H01.158.201.486.512', 'H01.181.122.573.512', 'H01.181.122.605.512'], ['G05.558'], ['G15.610'], ['B01.650.660'], ['B01.650.940.800.575.912.250.908.500.900'], ['B04.715.464.725.800', 'B04.820.578.844.800'], ['D12.776.964.970']]
|
['Anatomy [A]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Organisms [B]', 'Chemicals and Drugs [D]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
[Liver transplantation in hepatocellular carcinoma: new developments].
|
Today, liver transplantation is the only simultaneous treatment of cirrhosis as well as of HCC. A crucial factor for outcome after liver transplantation is the appropriate selection of patients. Tumor diameter and node number in correlation with the histopathological grading are used as surrogate markers to predict the prognostically relevant vascular invasion. Many centers accept patients suffering from a HCC with a maximum diameter of 5 cm and up to 3 tumor nodes. Total hepatectomy and liver transplantation for patients suffering from small hepatocellular carcinoma in cirrhosis result in 5- and 10-year-survival rates of 70% and 60%, respectively. This result reflects low rates of postoperative mortality and tumor recurrence as well as a decreased mortality due to the underlying cirrhosis. In multivariate analyses, vascular invasion and histopathological grading were significant risk factors for patient survival. Histopathological grading was also identified as most important risk factor for vascular invasion.
|
['Carcinoma, Hepatocellular', 'Humans', 'Liver Neoplasms', 'Liver Transplantation', 'Lymphatic Metastasis', 'Neoplasm Staging', 'Prognosis', 'Survival Rate']
| 12,233,282
|
[['C04.557.470.200.025.255', 'C04.588.274.623.160', 'C06.301.623.160', 'C06.552.697.160'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C04.588.274.623', 'C06.301.623', 'C06.552.697'], ['E02.095.147.725.490', 'E04.210.650', 'E04.936.450.490', 'E04.936.580.490'], ['C04.697.650.560', 'C23.550.727.650.560'], ['E01.789.625'], ['E01.789'], ['E05.318.308.985.550.900', 'N01.224.935.698.826', 'N06.850.505.400.975.550.900', 'N06.850.520.308.985.550.900']]
|
['Diseases [C]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Comparison of Laryngeal Mask Airway Supreme and Laryngeal Mask Airway Proseal with respect to oropharyngeal leak pressure during laparoscopic cholecystectomy: a randomised controlled trial.
|
CONTEXT: A comparison of the efficacy and safety of the Laryngeal Mask Airway (LMA) Supreme (LMAS) versus the LMA Proseal (LMAP) in elective laparoscopic cholecystectomy.OBJECTIVES: To compare the LMAS with LMAP in terms of ventilatory efficacy, airway leak pressure (airway protection), ease-of-use and complications.DESIGN: Prospective, single-blind, randomised, controlled study.SETTING: The Hospital del Sureste and Hospital Ramon y Cajal, Madrid, between May 2009 and March 2011. The Hospital del Sureste is a secondary hospital and Hospital Ramon y Cajal is a tertiary hospital.PATIENTS: Patients undergoing elective laparoscopic cholecystectomy were studied following informed consent. Inclusion criteria were American Society of Anesthesiologists physical status I to III and age 18 or more. Exclusion criteria were BMI more than 40 kg m, symptomatic hiatus hernia or severe gastro-oesophageal reflux.INTERVENTIONS: Anaesthesiologists experienced in the use of LMAP and LMAS participated in the trial. One hundred twenty-two patients were randomly allocated to LMAS or LMAP.MAIN OUTCOME MEASURES: Our primary outcome measure was the oropharyngeal leak pressure (OLP). Secondary outcomes were the time and number of attempts for insertion, ease of insertion of the drain tube, adequacy of ventilation and the incidence of complication. Patients were interviewed postoperatively to evaluate the presence of sore throat, dysphagia or dysphonia.RESULTS: Two patients were excluded when surgery changed from laparoscopic to open. A total of 120 patients were finally included in the analysis. The mean OLP in the LMAP group was significantly higher than that in the LMAS group (30.7 ± 6.2 versus 26.8 ± 4.1 cmH2O;P < 0.01). This was consistent with a higher maximum tidal volume achieved with the LMAP compared to the LMAS (511 ± 68 versus 475 ± 55 ml; P = 0.04). The success rate of the first attempt insertion was higher for the LMAS group than the LMAP group (96.7 and 71.2%, respectively; P < 0.01). The time taken for insertion, ease of insertion of the drain tube, complications and postoperative pharyngolaryngeal adverse events were similar in both groups.CONCLUSION: The LMAP has a higher OLP and achieves a higher maximum tidal volume compared to the LMAS, in patients undergoing elective laparoscopic cholecystectomy. The success of the first attempt insertion was higher for the LMAS.
|
['Adult', 'Aged', 'Anesthesia, General', 'Anesthesiology', 'Cholecystectomy, Laparoscopic', 'Equipment Design', 'Female', 'Humans', 'Intubation, Intratracheal', 'Laryngeal Masks', 'Male', 'Middle Aged', 'Pressure', 'Prospective Studies', 'Single-Blind Method', 'Tidal Volume', 'Treatment Outcome']
| 23,318,811
|
[['M01.060.116'], ['M01.060.116.100'], ['E03.155.197'], ['H02.403.066'], ['E04.210.120.172.140', 'E04.502.250.520.160'], ['E05.320'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.041.500', 'E02.585.578', 'E05.497.578'], ['E02.041.500.475', 'E02.585.578.475', 'E05.497.578.475', 'E07.700.500.450', 'J01.637.708.560.782.450'], ['M01.060.116.630'], ['G01.374.715'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['E05.318.370.850', 'N05.715.360.325.730', 'N06.850.520.445.850'], ['E01.370.386.700.485.750.900.350.750', 'G09.772.850.970.500.700'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Organisms [B]', 'Technology, Industry, and Agriculture [J]', 'Phenomena and Processes [G]', 'Health Care [N]']
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 1
| 0
|
Racial disparity in stroke risk factors: the Berlin-Ibadan experience; a retrospective study.
|
BACKGROUND: Different workers have reported racial disparities in the distribution of risk factors for stroke and stroke subtype (ischemic vs hemorrhagic). No transcultural transnational studies have been conducted to confirm and relate these disparities to one another. Our objective was to identify differences in the distribution of risk factors for stroke and stroke subtypes among urban-dwelling stroke patients in Nigeria, a developing country, and Germany, an industrialized country.METHODS: Consecutive stroke patients in Ibadan (100) and Berlin (103) were studied. Their hospital records were screened to identify documented vascular risk factors and stroke subtype.RESULTS: The stroke patients in Ibadan were younger than those in Berlin (t = 4.940, P = 0.000). Hypertension was significantly more common in Ibadan while cigarette smoking, dyslipidemia, atherosclerosis, and cardiac factors were significantly more frequent in Berlin. Cerebral infarction was more common in Berlin (80%) than in Ibadan (63%).CONCLUSION: The risk factors associated with cerebral infarction were more frequent in Berlin. We suspect that racial disparity in risk factors for stroke may account for the difference in proportions of stroke subtype in black and white populations. Larger prospective community-based multinational multiracial studies are required to confirm these disparities and identify possible underlying genetic, dietary, and socio-economic factors.
|
['Adult', 'African Continental Ancestry Group', 'Aged', 'Aged, 80 and over', 'Atherosclerosis', 'Berlin', 'Cerebral Infarction', 'Demography', 'Dyslipidemias', 'European Continental Ancestry Group', 'Female', 'Humans', 'Hypertension', 'Male', 'Middle Aged', 'Nigeria', 'Pilot Projects', 'Retrospective Studies', 'Risk Factors', 'Smoking', 'Stroke']
| 18,638,038
|
[['M01.060.116'], ['M01.686.508.100'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['C14.907.137.126.307'], ['Z01.433.128', 'Z01.542.315.182'], ['C10.228.140.300.150.477.200', 'C10.228.140.300.775.200.200', 'C14.907.253.092.477.200', 'C14.907.253.855.200.200', 'C23.550.513.355.250.200', 'C23.550.717.489.250.200'], ['I01.240', 'N01.224', 'N06.850.505.400'], ['C18.452.584.500'], ['M01.686.508.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C14.907.489'], ['M01.060.116.630'], ['Z01.058.290.190.565'], ['E05.318.372.750', 'E05.337.737', 'N05.715.360.330.720', 'N06.850.520.450.720'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['F01.145.805'], ['C10.228.140.300.775', 'C14.907.253.855']]
|
['Named Groups [M]', 'Diseases [C]', 'Geographicals [Z]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Psychiatry and Psychology [F]']
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 1
|
Selective brain hypothermia suppresses noxious-evoked movement in canines.
|
Systemic hypothermia suppresses noxious-evoked movement, but its main site of action is unknown. We examined the effect of hypothermia in the brain on noxious-evoked movement by selectively cooling the brain. Sixteen beagles were randomly divided into two groups and anesthetized with isoflurane/oxygen. After being deeply anesthetized, the dogs' lungs were artificially ventilated, and several major vessels were cannulated for perfusion and monitoring. Cold lactate Ringer's solution was infused into the right vertebral artery to cool the brain while maintaining the trunk temperature relatively warmer. When the brain temperature decreased to 20 degrees C or 25 degrees C, isoflurane administration was discontinued; the trunk temperatures at this stage were approximately 34.7 degrees C and 34.6 degrees C, respectively. After the end-tidal isoflurane concentration reached 0%, the base of the tail was stimulated with an electric current through 2 25-gauge needles. None of the dogs reacted to tail stimulation when the brain temperature was at 20 degrees C, whereas 7 of 8 reacted at 25 degrees C. These results indicate that selective brain hypothermia (20 degrees C) results in suppressing noxious-evoked movement in canines.
|
['Animals', 'Blood Pressure', 'Body Temperature', 'Brain', 'Dogs', 'Electroencephalography', 'Female', 'Hypothermia, Induced', 'Movement', 'Pain']
| 15,845,705
|
[['B01.050'], ['E01.370.600.875.249', 'G09.330.380.076'], ['E01.370.600.875.374', 'G07.110'], ['A08.186.211'], ['B01.050.150.900.649.313.750.250.216.200'], ['E01.370.376.300', 'E01.370.405.245'], ['E02.258.750'], ['G07.568', 'G11.427.410'], ['C23.888.592.612', 'F02.830.816.444', 'G11.561.790.444']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Diseases [C]', 'Psychiatry and Psychology [F]']
| 1
| 1
| 1
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Examination of IMPA1 and IMPA2 genes in manic-depressive patients: association between IMPA2 promoter polymorphisms and bipolar disorder.
|
Manic-depressive (bipolar) illness is a serious psychiatric disorder with a strong genetic predisposition. The disorder is likely to be multifactorial and etiologically complex, and the causes of genetic susceptibility have been difficult to unveil. Lithium therapy is a widely used pharmacological treatment of manic-depressive illness, which both stabilizes the ongoing episodes and prevents relapses. A putative target of lithium treatment has been the inhibition of the myo-inositol monophosphatase (IMPase) enzyme, which dephosphorylates myo-inositol monophosphate in the phosphatidylinositol signaling system. Two genes encoding human IMPases have so far been isolated, namely myo-inositol monophosphatase 1 (IMPA1) on chromosome 8q21.13-21.3 and myo-inositol monophosphatase 2 (IMPA2) on chromosome 18p11.2. In the present study, we have scanned for DNA variants in the human IMPA1 and IMPA2 genes in a pilot sample of Norwegian manic-depressive patients, followed by examination of selected polymorphisms and haplotypes in a family-based bipolar sample of Palestinian Arab proband-parent trios. Intriguingly, two frequent single-nucleotide polymorphisms (-461C>T and -207T>C) in the IMPA2 promoter sequence and their corresponding haplotypes showed transmission disequilibrium in the Palestinian Arab trios. No association was found between the IMPA1 polymorphisms and bipolar disorder, neither with respect to disease susceptibility nor with variation in lithium treatment response. The association between manic-depressive illness and IMPA2 variants supports several reports on the linkage of bipolar disorder to chromosome 18p11.2, and sustains the possible role of IMPA2 as a susceptibility gene in bipolar disorder.
|
['Base Sequence', 'Bipolar Disorder', 'Chromosome Mapping', 'Chromosomes, Human, Pair 18', 'Chromosomes, Human, Pair 8', 'DNA Primers', 'Humans', 'Norway', 'Phosphoric Monoester Hydrolases', 'Polymorphism, Single Nucleotide', 'Promoter Regions, Genetic']
| 14,699,425
|
[['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['F03.084.500'], ['E05.393.183'], ['A11.284.187.520.300.415.430', 'G05.360.162.520.300.415.430'], ['A11.284.187.520.300.325.340', 'G05.360.162.520.300.325.340'], ['D13.695.578.424.450.275', 'D27.720.470.530.600.223.600'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['Z01.542.816.374'], ['D08.811.277.352.650'], ['G05.365.795.598'], ['G02.111.570.080.689.675', 'G05.360.080.689.675', 'G05.360.340.024.340.137.750.680']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Psychiatry and Psychology [F]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Geographicals [Z]']
| 1
| 1
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 1
|
Effects of barium concentration on the radiopacity and biomechanics of bone cement: experimental study.
|
PURPOSE: This study was undertaken to evaluate the changes in the radiopacity and mechanics of polymethylmethacrylate (PMMA) bone cement with the addition of barium.MATERIALS AND METHODS: Barium sulfate powder was added to a PMMA bone cement with an initial 10% barium concentration. The changes in radiopacity and strength were evaluated by testing cement blocks containing four barium concentrations (10%, 20%, 30%, 40%). Radiopacity was evaluated by measuring the computed tomography (CT) values of the bone cement, and strength was evaluated by compressive, three-point bending, and impact load tests.RESULTS: CT values increased in proportion to the barium concentration. The compressive load test showed that cement with a 40% barium concentration was significantly more fragile than cement with lower barium concentrations. The three-point bending load test showed that the cement became more fragile in proportion to the barium concentration. The impact load test showed that cement with 30% and 40% barium concentrations was significantly more fragile than cement with 10% and 20% barium concentrations.CONCLUSION: Radiopacity is increased and strength is reduced by adding increasing concentrations of barium powder to bone cement. The results of the present study suggest that adding barium permits the radiopacity and strength of bone cement to be adjusted in clinical practice.
|
['Barium Sulfate', 'Biomechanical Phenomena', 'Bone Cements', 'Compressive Strength', 'Image Processing, Computer-Assisted', 'Materials Testing', 'Polymethyl Methacrylate', 'Radiographic Image Enhancement', 'Stress, Mechanical', 'Tensile Strength', 'Tomography Scanners, X-Ray Computed']
| 19,030,961
|
[['D01.103.075', 'D01.875.800.800.850.075'], ['G01.154.090', 'G01.374.089'], ['D05.750.716.822.300', 'D25.720.716.822.300', 'D27.720.102.158', 'J01.637.051.720.716.822.300'], ['G01.374.180'], ['L01.224.308'], ['E05.570'], ['D02.241.081.069.800.550.500', 'D05.750.716.822.111.650.605.500', 'D25.720.716.822.111.650.605.500', 'J01.637.051.720.716.822.111.650.605.500'], ['E01.370.350.600.350.700', 'E01.370.350.700.700', 'L01.224.308.380.600'], ['G01.374.835'], ['G01.374.850'], ['E07.913']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Technology, Industry, and Agriculture [J]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 0
|
Some results for an SEIR epidemic model with density dependence in the death rate.
|
This paper deals with an SEIR epidemic model for an infectious disease where the death rate depends on the number of individuals in the population. It is also assumed that there is an additional death rate suffered by infected individuals. It is found that there are three steady-state values: one where the population is extinct, one where the population maintains itself at a constant level and the disease is extinct, and one where there is a unique equilibrium with disease present. An interesting and unusual feature is that it is possible for this third equilibrium to exist and be locally unstable. Numerical work and simulation show that we can have cycles of disease incidence with increasing amplitude, a constant amplitude, and a decreasing amplitude, depending on the parameter values of the model.
|
['Animals', 'Communicable Diseases', 'Disease Outbreaks', 'Humans', 'Mathematics', 'Models, Biological', 'Mortality', 'Population Density']
| 1,517,675
|
[['B01.050'], ['C01.221', 'C23.550.291.531'], ['N06.850.290'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['H01.548'], ['E05.599.395'], ['E05.318.308.985.550', 'N01.224.935.698', 'N06.850.505.400.975.550', 'N06.850.520.308.985.550'], ['N01.224.600', 'N06.850.505.400.600']]
|
['Organisms [B]', 'Diseases [C]', 'Health Care [N]', 'Disciplines and Occupations [H]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
|
Adult rhabdomyoma of the extremity.
|
Adult rhabdomyoma is a rare benign tumor. It mainly occurs in the head and neck region and rarely occurs outside the head and neck region. We present an extremely rare case of the adult rhabdomyoma arising in the left foot in a 46-year-old male. Microscopically, large polygonal cells and large strap-shaped cells were observed. This is the third case of adult rhabdomyoma arising in an extremity.
|
['Foot Diseases', 'Humans', 'Male', 'Middle Aged', 'Neoplasm Recurrence, Local', 'Rhabdomyoma']
| 24,021,898
|
[['C05.360', 'C17.800.321'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['C04.697.655', 'C23.550.727.655'], ['C04.557.450.590.540.700']]
|
['Diseases [C]', 'Organisms [B]', 'Named Groups [M]']
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Relationship between photosensitizing activities and chemical structure of hypocrellin A and B.
|
Hypocrellins A (HA) and B (HB) are two main pigments isolated from the parasitic fungi Hypocrella bambusae (B. et Br) Sacc. and Shiraia bambusicola P. Heen found in China. These pigments have a long history as traditional medicinal agents. Although HA and HB have the same perylene quinonoid structure, there are different side rings. The different photosensitizing activities of HA and HB due to their different side rings were studied by electron paramagnetic resonance (EPR), spin trapping and spin counteraction techniques. It is demonstrated that the ability to generate active oxygen (1O2, O2.- and .OH) is stronger during HA photosensitization than during HB photosensitization. Under anaerobic conditions, the ability to generate HA.- during HA photosensitization is stronger than the ability to generate HB.- during HB photosensitization. There is a relationship between photosensitizing ability and chemical structure.
|
['Cyclic N-Oxides', 'Drugs, Chinese Herbal', 'Electron Spin Resonance Spectroscopy', 'Free Radicals', 'Light', 'Oxygen', 'Perylene', 'Phenol', 'Quinones', 'Radiation-Sensitizing Agents', 'Spin Labels', 'Structure-Activity Relationship']
| 1,331,387
|
[['D03.661.243'], ['D20.215.784.500.350', 'D26.335'], ['E05.196.867.519.274'], ['D01.339', 'D02.389'], ['G01.358.500.505.650', 'G01.590.540', 'G01.750.250.650', 'G01.750.770.578'], ['D01.268.185.550', 'D01.362.670'], ['D02.455.426.559.847.149.700', 'D02.455.426.559.847.680.500', 'D04.615.149.700', 'D04.615.680.500'], ['D02.455.426.559.389.657.595'], ['D02.806'], ['D27.505.954.600'], ['D02.389.678'], ['G02.111.830', 'G07.690.773.997']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Adenosarcoma of the uterus: ultrastructural observations.
|
M?llerian adenosarcoma has been recently described as a distinctive biphasic uterine tumor with benign epithelial and malignant stromal components. The lesion belongs generally to the group of mixed m?llerian tumors, but the benignity of the epithelium separates it from the other neoplasms of this type. This entity usually affects postmenopausal patients who follow a protracted course. This paper reports a case fulfilling the original clinical and pathologic description of Clement and Scully and adds ultrastructural observations. Electron microscopic examination confirmed the optical impression of a biphasic neoplasm with benign epithelial and malignant stromal components. The epithelium resembled proliferative endometrium, and the stroma had features of endometrial stromal sarcoma or mixed m?llerian tumor. The findings of stromal resemblance to endometrial stromal sarcoma or mixed m?llerian tumor and the presence of a variety of m?llerian epithelia support the proposed m?llerian derivation of this lesion.
|
['Aged', 'Female', 'Humans', 'Microscopy, Electron', 'Uterine Neoplasms', 'Wilms Tumor']
| 217,264
|
[['M01.060.116.100'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.350.515.402', 'E05.595.402'], ['C04.588.945.418.948', 'C13.351.500.852.762', 'C13.351.937.418.875'], ['C04.557.435.595', 'C04.588.945.947.535.585', 'C04.700.900', 'C12.758.820.750.585', 'C12.777.419.473.585', 'C13.351.937.820.535.585', 'C13.351.968.419.473.585', 'C16.320.700.900']]
|
['Named Groups [M]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Erythropoietin ameliorates diabetes-associated cognitive dysfunction in vitro and in vivo.
|
Several studies indicate that erythropoietin (EPO) has remarkable neuroprotective effects in various central nervous system disorders, while little is known about the effects of EPO in diabetes-associated cognitive dysfunction. Therefore, the present study aimed to investigate whether EPO ameliorates diabetes-associated cognitive dysfunction in vivo and in vitro. We investigated the protective effects of EPO on high-glucose (HG)-induced PC12 cell death and oxidative stress. The effects of EPO (300 U/kg administered three times a week for 4 weeks) on diabetes-associated cognitive decline were investigated in diabetic rats. EPO significantly increased cell viability, increased the activity of superoxide dismutase, decreased the production of malondialdehyde and reactive oxygen species, and decreased the apoptosis rate. Additionally, LY294002, a phosphatidylinositol 3-kinase (PI3K) inhibitor, abolished the protective effects of EPO in HG-treated PC12 cells. In diabetic rats, EPO prevented deficits in spatial learning and memory in the Morris water maze test. The results of real-time PCR and Western blotting showed that EPO upregulated EPO receptor, PI3K, and phosphorylated Akt2 relative to unphosphorylated Akt2 (p-Akt2/Akt2) and downregulated glycogen synthase kinase-3â (GSK-3â). These studies demonstrate that EPO is an effective neuroprotective agent in the context of diabetes-associated cognitive dysfunction and show that this effect involves the PI3K/Akt/GSK-3â pathway.
|
['Animals', 'Apoptosis', 'Cell Survival', 'Cognitive Dysfunction', 'Diabetes Complications', 'Diabetes Mellitus, Experimental', 'Disease Models, Animal', 'Erythropoietin', 'Glucose', 'Glycogen Synthase Kinase 3 beta', 'Models, Biological', 'Neuroprotective Agents', 'Oxidative Stress', 'PC12 Cells', 'Phosphatidylinositol 3-Kinases', 'Proto-Oncogene Proteins c-akt', 'Rats', 'Reactive Oxygen Species', 'Receptors, Erythropoietin', 'Signal Transduction']
| 28,584,284
|
[['B01.050'], ['G04.146.954.035'], ['G04.346'], ['F03.615.250.700'], ['C19.246.099'], ['C18.452.394.750.074', 'C19.246.240', 'E05.598.500.374'], ['C22.232', 'E05.598.500', 'E05.599.395.080'], ['D12.644.276.374.410.240.150', 'D12.776.395.240.150', 'D12.776.467.374.410.240.150', 'D23.529.374.410.240.150'], ['D09.947.875.359.448'], ['D05.500.117.875.500', 'D08.811.913.696.620.682.700.429.500.500', 'D08.811.913.696.620.682.700.646.625.500', 'D12.644.360.300.500.500', 'D12.776.476.081.875.500', 'D12.776.476.300.500.500'], ['E05.599.395'], ['D27.505.696.706.548', 'D27.505.954.427.575'], ['G03.673', 'G07.775.750'], ['A11.251.210.190.750', 'A11.251.860.180.750', 'A11.299.500'], ['D08.811.913.696.620.500'], ['D08.811.913.696.620.682.700.755', 'D12.776.476.565', 'D12.776.624.664.700.168'], ['B01.050.150.900.649.313.992.635.505.700'], ['D01.339.431', 'D01.650.775'], ['D12.776.543.750.705.852.150.200', 'D12.776.543.750.750.400.200.340'], ['G02.111.820', 'G04.835']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Psychiatry and Psychology [F]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Mortality in patients with Crohn's disease.
|
BACKGROUND: No data on mortality for Crohn's disease are available from southern Europe.METHODS: Five hundred and thirty-one patients with Crohn's disease were observed in our unit between 1973 and 1993. In 325 patients the first diagnosis was made in our hospital. In this consecutive incidence series, in which the follow-up was 99% complete, the standardized mortality rate (SMR) was calculated.RESULTS: Nine deaths were observed, against 9.25 expected. The SMR was 0.97 (95% confidence interval (CI), 0.4-1.8). The relative risk of dying was significantly higher in the female group in the first 5 years after diagnosis (SMR, 10.3; 95% CI, 2.30-30.2). There was an excess of deaths from tumors of the digestive organs (1 observed, 0.37 expected).CONCLUSIONS: These results show that in our geographic area the mortality from Crohn's disease was not increased as shown in other community studies.
|
['Adolescent', 'Adult', 'Aged', 'Cause of Death', 'Child', 'Crohn Disease', 'Europe', 'Female', 'Humans', 'Male', 'Middle Aged', 'Survival Rate']
| 8,726,306
|
[['M01.060.057'], ['M01.060.116'], ['M01.060.116.100'], ['E05.318.308.985.550.250', 'N01.224.935.698.100', 'N06.850.505.400.975.550.250', 'N06.850.520.308.985.550.250'], ['M01.060.406'], ['C06.405.205.731.500', 'C06.405.469.432.500'], ['Z01.542'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['E05.318.308.985.550.900', 'N01.224.935.698.826', 'N06.850.505.400.975.550.900', 'N06.850.520.308.985.550.900']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Diseases [C]', 'Geographicals [Z]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
[Changes in the transaldolase activity in the liver, spleen and bone marrow of rats after total X-ray irradiation].
|
The paper deals with changes in the activity of transaldolase in tissues of the liver, spleen and bone marrow of rats 4.24 h and on the 3 d, 7th, 18th, 30th, 45th, and 60th day after total X-ray irradiation in a dose of 600 R. The ionizing irradiation causes a stable inhibition of the enzyme activity in All tissues. The observed changes are homogeneous in their direction but have their own peculiarities for each tissue. Differences in the degree and extent of the enzyme activity changes at various stages of the X-ray lesion development in the tissues under study may be explained by their different sensitivity to the penetrating radiation.
|
['Animals', 'Bone Marrow', 'Kinetics', 'Liver', 'Male', 'Rats', 'Spleen', 'Transaldolase', 'Transferases', 'Whole-Body Irradiation']
| 7,036,489
|
[['B01.050'], ['A15.382.216'], ['G01.374.661', 'G02.111.490'], ['A03.620'], ['B01.050.150.900.649.313.992.635.505.700'], ['A10.549.700', 'A15.382.520.604.700'], ['D08.811.913.200.650'], ['D08.811.913'], ['E02.815.814', 'E05.980']]
|
['Organisms [B]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Development and testing of the autobiographical memory coding tool.
|
Development and testing of the autobiographical memory coding tool (AMCT) is detailed. The tool uses quantitative content analysis procedures to code interpretations of autobiographical memories as validating or lamenting. Development of a system of measuring autobiographical memories from transcribed reminiscence interviews involved defining the units of analysis, defining the categories and themes, constructing a codebook, assessing content validity, assessing reliability and making revisions. Thirty-nine transcripts of reminiscence interviews were used for tool development and testing. The AMCT contains a series of step-by-step guidelines for conducting the analysis and includes an autobiographical memory thematic dictionary and descriptions of range and variations in each theme to assist with coding data. Intercoder reliability estimates were 0.93, 0.93 and 0.95. Test-retest reliability was 1.00.
|
['Abstracting and Indexing', 'Aged', 'Aged, 80 and over', 'Autobiographies as Topic', 'Choice Behavior', 'Female', 'Geriatric Nursing', 'Grief', 'Happiness', 'Humans', 'Life Change Events', 'Male', 'Nursing Methodology Research', 'Personal Satisfaction', 'Reproducibility of Results', 'Self Concept', 'Social Support']
| 8,496,515
|
[['L01.453.245.100'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['K01.517.211.215'], ['F02.463.785.373.346'], ['H02.478.676.236', 'N02.421.533.245'], ['F01.470.142.110'], ['F01.470.516'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F01.829.458.410'], ['H01.770.644.145.390.634', 'H02.478.395.634', 'N04.590.233.508.613.634'], ['F01.145.677'], ['E05.318.370.725', 'E05.337.851', 'N05.715.360.325.685', 'N06.850.520.445.725'], ['F01.752.747.792'], ['I01.880.853.500.600']]
|
['Information Science [L]', 'Named Groups [M]', 'Humanities [K]', 'Psychiatry and Psychology [F]', 'Disciplines and Occupations [H]', 'Health Care [N]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anthropology, Education, Sociology, and Social Phenomena [I]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 1
| 0
|
Children's adjustment and prosocial behaviour in step-, single-parent, and non-stepfamily settings: findings from a community study. ALSPAC Study Team. Avon Longitudinal Study of Pregnancy and Childhood.
|
The adjustment and prosocial behaviour of 4-year-old children and their older siblings growing up in step-parent or single-parent families, or with two biological parents, was investigated within a longitudinal community study, the Avon Longitudinal Study of Pregnancy and Childhood (ALSPAC). Mean differences in mothers' perception of adjustment were found for children in different family settings, with higher levels of problems and lower prosocial scores reported for those in single- and step-parent families than those in non-stepfamilies. Individual differences within each family setting were marked. With the exception of single parenthood, which remained a risk indicator for the 4-year-olds, the contribution of family type to differences in adjustment and prosocial behaviour largely disappeared when account was also taken of negativity in family relationships, maternal age, education level, depressive symptomatology, and history of previous live-in relationships, mothers' support networks, and the family's current financial and housing circumstances. Boys remained more at risk for adjustment difficulties than girls when this range of factors was taken into account. The limitations and implications of these findings on a community sample, a first step in a programme of research into family processes in children's adjustment, are discussed.
|
['Adolescent', 'Adult', 'Child', 'Child Behavior Disorders', 'Child, Preschool', 'Family', 'Family Characteristics', 'Family Relations', 'Female', 'Humans', 'Longitudinal Studies', 'Male', 'Parent-Child Relations', 'Parents', 'Regression Analysis', 'Sampling Studies', 'Sex Characteristics', 'Single Parent', 'Social Adjustment', 'Social Behavior', 'Socioeconomic Factors']
| 9,844,979
|
[['M01.060.057'], ['M01.060.116'], ['M01.060.406'], ['F03.625.141'], ['M01.060.406.448'], ['F01.829.263', 'I01.880.853.150'], ['F01.829.263.315', 'I01.240.361', 'I01.880.853.150.423', 'N01.224.361', 'N01.824.308', 'N06.850.505.400.400'], ['F01.829.263.370', 'I01.880.853.150.439'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.372.500.750.500', 'N05.715.360.330.500.750.500', 'N06.850.520.450.500.750.500'], ['F01.829.263.370.290'], ['F01.829.263.500.320', 'I01.880.853.150.500.340', 'M01.620'], ['E05.318.740.750', 'N05.715.360.750.695', 'N06.850.520.830.750'], ['E05.318.372.875', 'N05.715.360.330.875', 'N06.850.520.450.875'], ['G08.686.815'], ['F01.829.263.315.500.725.700', 'F01.829.263.500.320.785', 'I01.240.361.500.725.700', 'I01.880.853.150.423.500.725.700', 'I01.880.853.150.500.340.785', 'M01.620.785', 'N01.224.361.500.725.700', 'N01.824.308.500.725.700'], ['F01.145.813.621'], ['F01.145.813'], ['I01.880.853.996', 'N01.824']]
|
['Named Groups [M]', 'Psychiatry and Psychology [F]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 0
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 0
|
[An unusual case of cystic nephroma protruding into the renal pelvis].
|
Cystic nephroma is a relatively rare and benign renal lesion of uncertain etiology. Approximately 200 cases have been described, but only a few cases of cystic nephroma with prominent renal pelvis involvement have been reported. Here, we report an unusual case of cystic nephroma that protruded into the renal pelvis. A 45-year-old woman hospitalized because of a right renal mass detected on clinical examination. The diagnosis of a malignant or benign lesion was not clear. A right nephroureterectomy was performed. The characteristics of the resected specimen were consistent with those of cystic nephroma. Macroscopic examination revealed that the giant lesion originated from the upper renal pelvis and extended into the renal pelvis. The patient is currently free from disease at eight months after the surgery. In general, this tumor arises from the renal parenchyma. To our knowledge, this is the fifth case report on cystic nephroma with prominent renal pelvis involvement.
|
['Female', 'Humans', 'Kidney Diseases, Cystic', 'Kidney Pelvis', 'Middle Aged']
| 21,063,163
|
[['B01.050.150.900.649.313.988.400.112.400.400'], ['C12.777.419.403', 'C13.351.968.419.403'], ['A05.810.453.537'], ['M01.060.116.630']]
|
['Organisms [B]', 'Diseases [C]', 'Anatomy [A]', 'Named Groups [M]']
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Characterization of 58-kilodalton human neutrophil collagenase: comparison with human fibroblast collagenase.
|
A series of experiments has been carried out to characterize 58-kDa human neutrophil collagenase (HNC) and compare it with human fibroblast collagenase (HFC). N-Terminal sequencing of latent and spontaneously activated HNC shows that it is a distinct collagenase that is homologous to HFC and other members of the matrix metalloproteinase gene family. Activation occurs autolytically by hydrolysis of an M-L bond at a locus homologous to the Q80-F81-V82-L83 autolytic activation site of HFC. This releases a 16-residue propeptide believed to contain the "cysteine switch" residue required for latency. Polyclonal antibody raised against HNC cross-reacts with HFC but with none of the other major human matrix metalloproteinases examined. Treatment of HNC with endoglycosidase F or N-glycosidase F indicates that it is glycosylated at multiple sites. The deglycosylated latent and spontaneously activated enzymes have molecular weights of approximately 44K and 42K, respectively. Differences in the carbohydrate processing of HFC and HNC may determine why HFC is a secreted protein while HNC is stored in intracellular granules. The kinetic parameters kcat and KM for the hydrolysis of the interstitial collagen types I, II, and III in solution by both collagenases have been determined. The strong preferences of HNC for type I collagen and of HFC for type III collagen found in earlier studies have been confirmed. The preference of HNC for type I over type III collagen is almost abolished when fibrillar collagens are used as substrates, but the preference for HFC for type III over type I collagen is only partially decreased.(ABSTRACT TRUNCATED AT 250 WORDS)
|
['Amino Acid Sequence', 'Blotting, Western', 'Cross Reactions', 'Fibroblasts', 'Glycoproteins', 'Humans', 'Microbial Collagenase', 'Molecular Sequence Data', 'Molecular Weight', 'Neutrophils', 'Protein Processing, Post-Translational', 'Substrate Specificity']
| 2,176,876
|
[['G02.111.570.060', 'L01.453.245.667.060'], ['E05.196.401.143', 'E05.301.300.096', 'E05.478.566.320.200', 'E05.601.262', 'E05.601.470.320.200'], ['G12.122.281'], ['A11.329.228'], ['D09.400.430', 'D12.776.395'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D08.811.277.656.300.480.205.500', 'D08.811.277.656.675.374.205.500'], ['L01.453.245.667'], ['G02.494'], ['A11.118.637.415.583', 'A11.627.340.583', 'A11.733.689', 'A15.145.229.637.415.583', 'A15.382.490.315.583', 'A15.382.680.689'], ['G02.111.660.871.790.600', 'G02.111.691.600', 'G03.734.871.790.600', 'G05.308.670.600'], ['G02.111.835']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Organisms [B]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
[7-alpha-Hydroxylation of 5 alpha-androstane-3 beta, 17 beta-diol in human normal and hyperplastic prostates].
|
Incubation of 4-14C-5 alpha-androstane-3 beta, 17 beta-diol with human normal and hyperplastic prostate minces led to the NADPH-dependent production of a major polar radiometabolite which was identified as 5 alpha-androstane-3 beta, 7 alpha, 17 beta-triol and proved the presence of a 3 beta-hydroxysteroid-7 alpha-hydroxylase in prostate. Such evidences may bring new leads for the study of the mechanisms of androgenic steroid action in the prostate gland.
|
['Adolescent', 'Androstane-3,17-diol', 'Androstanes', 'Humans', 'Male', 'Prostate', 'Prostatic Hyperplasia', 'Steroid Hydroxylases']
| 71,955
|
[['M01.060.057'], ['D04.210.500.054.040.080', 'D06.472.334.851.968.500'], ['D04.210.500.054'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A05.360.444.575', 'A10.336.707'], ['C12.294.565.500'], ['D08.244.453.915', 'D08.811.682.690.708.170.915', 'D12.776.422.220.453.915']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Anatomy [A]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Consecutive melatonin circadian rhythms in normal volunteers.
|
Circadian plasma melatonin secretion patterns were determined in 12 healthy men over 3 consecutive 24-hour periods. The rhythms showed marked intra-individual stability, but pronounced interindividual variation. Plasma melatonin may serve as a reliable biological marker. Plasma melatonin assayed between 23h00 and 01h00 gave a reliable indication of an individual's circadian melatonin rhythm.
|
['Adult', 'Circadian Rhythm', 'Humans', 'Light', 'Male', 'Melatonin', 'Reference Values']
| 2,919,339
|
[['M01.060.116'], ['G07.180.562.190'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G01.358.500.505.650', 'G01.590.540', 'G01.750.250.650', 'G01.750.770.578'], ['D03.633.100.473.914.481', 'D06.472.506'], ['E05.978.810']]
|
['Named Groups [M]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Design, synthesis, and evaluation of hydroxamic acid derivatives as promising agents for the management of Chagas disease.
|
Today, there are approximately 8 million cases of Chagas disease in the southern cone of South America alone, and about 100 million people are living with the risk of becoming infected. The present pharmacotherapy is sometimes ineffective and has serious side effects. Here, we report a series of 4,5-dihydroisoxazoles incorporating hydroxamate moieties, which act as effective inhibitors of the carbonic anhydrase (CA) from Trypanosoma cruzi (TcCA). One compound (5g) was evaluated in detail and shows promising features as an antitrypanosomal agent. Excellent values for the inhibition of growth for all three developmental forms of the parasite were observed at low concentrations of 5g (IC50 values from 7.0 to <1 ìM). The compound has a selectivity index (SI) of 6.7 and no cytotoxicity to macrophage cells. Preliminary in vivo data showed that 5g reduces bloodstream parasites and that all treated mice survived; it was also more effective than the standard drug benznidazole.
|
['Animals', 'Carbonic Anhydrase Inhibitors', 'Carbonic Anhydrases', 'Cell Line, Tumor', 'Chagas Disease', 'Drug Design', 'Humans', 'Hydroxamic Acids', 'Isoenzymes', 'Isoxazoles', 'Macrophages, Peritoneal', 'Mice', 'Mice, Inbred BALB C', 'Peptide Hydrolases', 'Protease Inhibitors', 'Structure-Activity Relationship', 'Trypanocidal Agents', 'Trypanosoma cruzi']
| 24,299,463
|
[['B01.050'], ['D27.505.519.389.200'], ['D08.811.520.241.300.150'], ['A11.251.210.190', 'A11.251.860.180'], ['C01.610.752.300.900.200', 'C01.920.625'], ['E05.290.500', 'H01.158.703.007.338.500', 'H01.181.466.338.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D02.092.570.394', 'D02.241.511.372'], ['D08.811.348', 'D12.776.800.300'], ['D03.383.129.385'], ['A11.329.372.630', 'A11.627.482.630', 'A11.733.397.630', 'A15.382.670.522.630', 'A15.382.680.397.630'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.338', 'B01.050.150.900.649.313.992.635.505.500.400.338'], ['D08.811.277.656'], ['D27.505.519.389.745'], ['G02.111.830', 'G07.690.773.997'], ['D27.505.954.122.250.100.875'], ['B01.268.475.868.887.140']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
Biotypes and ScM types of isolates of Streptococcus canis
|
Lancefield group G Streptococcus canis is a component of the normal urogenital and pharyngeal flora of the cat. It is also frequently implicated in epizootics of severe disease in closed cat colonies and animal shelters. Given the importance of S canis as a feline pathogen and relative lack of published information on characteristics potentially associated with virulence, the authors have compared isolates from healthy and diseased cats in New York and California using fermentation profiles (biotype) and ScM sequences. With few exceptions, isolates associated with disease were biotype 1. Four alleles of scm were identified of which type 1 dominated in diseased cats. Type 4 allelic variants were found only in healthy cats and all but one were biotype 2. Type 2 and 3 alleles showed extensive N-terminal variation suggesting a plasminogen-binding site as found on the type 1 allele was absent. Cat antisera to ScM were opsonobactericidal, and these potentially protective antibodies increased during convalescence.
|
['Animals', 'Bacterial Typing Techniques', 'Cat Diseases', 'Cats', 'Streptococcal Infections', 'Streptococcus']
| 28,077,757
|
[['B01.050'], ['E01.370.225.875.150.125', 'E05.200.875.150.125'], ['C22.180'], ['B01.050.150.900.649.313.750.377.750.250.125'], ['C01.150.252.410.890'], ['B03.353.750.737.872', 'B03.510.400.800.872', 'B03.510.550.737.872']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Effect of trypan blue staining on the density and viability of lens epithelial cells in white cataract.
|
PURPOSE: To assess the effect of anterior capsule staining with trypan blue 0.0125% on the density and viability of the lens epithelial cells (LECs).SETTING: Iladevi Cataract and IOL Research Center, Ahmedabad, India.METHODS: This randomized prospective study comprised 40 eyes having phacoemulsification for white mature cataract. Patients were randomized to have the anterior capsule stained with 0.1 mL trypan blue 0.0125% solution before the anterior capsulorhexis (Group 1, n = 20) or to have no anterior capsule staining before the anterior capsulorhexis (Group 2, n = 20). The density and viability of LECs were analyzed. The first 20 samples (10 from Group 1, 10 from Group 2) were stained with hematoxylin-eosin for cell density analysis using image-analysis software. The remaining 20 samples (10 from each group) were stained with a solution containing 0.5 microM calcein AM and 5 microM propidium iodide for 10 minutes at 37 degrees C for viability assay.RESULTS: The mean cell density in Group 1 (with staining) was 3533.15 cells/mm(2) +/- 664.01 (SD). This was significantly lower than the mean of 4235.59 +/- 414.93 cells/mm(2) in Group 2 (no staining) (P = .01). The mean capsule area covered with LECs (80.75% +/- 8.04% versus 94.63% +/- 3.78%) and the mean live LECs in the covered area (51.69% +/- 11.54% versus 68.67% +/- 9.15%) were also significantly lower in Group 1 (P = .002).CONCLUSION: Staining the anterior capsule with trypan blue affected the density and viability of LECs.
|
['Capsulorhexis', 'Cataract', 'Cell Count', 'Cell Survival', 'Coloring Agents', 'Epithelial Cells', 'Female', 'Humans', 'Lens Capsule, Crystalline', 'Lens, Crystalline', 'Male', 'Middle Aged', 'Phacoemulsification', 'Prospective Studies', 'Staining and Labeling', 'Trypan Blue']
| 16,931,259
|
[['E04.540.825.249.352'], ['C11.510.245'], ['E01.370.225.500.195', 'E05.200.500.195', 'E05.242.195', 'G04.140'], ['G04.346'], ['D27.720.233'], ['A11.436'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A09.371.060.500.155'], ['A09.371.060.500'], ['M01.060.116.630'], ['E04.540.825.249.704', 'E04.943.875'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['E01.370.225.500.620.670', 'E01.370.225.750.600.670', 'E05.200.500.620.670', 'E05.200.750.600.670'], ['D02.172.975', 'D02.455.426.559.847.638.555.875', 'D02.886.645.600.080.050.650.875', 'D04.615.638.555.875']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Organisms [B]', 'Named Groups [M]', 'Health Care [N]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Adaptation of a trap door technique for the recovery of ovarian cortical biopsies from Cebus apella (capuchin monkey).
|
There is a paucity of efficient cryopreservation protocols for primordial follicles enclosed in the ovarian tissue from non-human primates (NHP), in special New World primates. Our objective was to establish an optimal procedure for the recovery of ovarian biopsies from capuchin monkeys. To this end, we adapted a trap door biopsy method. Follicular density and quality of the biopsies were evaluated and ultrasound analysis was performed before and continuously after surgery to assess ovarian structure. Ovarian tissue biopsies recovered by the trap door technique allowed the successful harvesting of primordial follicles from capuchin monkeys, and no complication was recorded. The female cycle was not affected by surgery and no adherence was found thereafter. In conclusion, the adaptation of a trap door biopsy method is a safe procedure and allows recovery of healthy primordial follicles.
|
['Animals', 'Biopsy', 'Cebus', 'Female', 'Humans', 'Microscopy', 'Ovarian Follicle', 'Reconstructive Surgical Procedures', 'Ultrasonography, Doppler, Duplex']
| 22,475,413
|
[['B01.050'], ['E01.370.225.500.384.100', 'E01.370.225.998.054', 'E01.370.388.100', 'E04.074', 'E05.200.500.384.100', 'E05.200.998.054', 'E05.242.384.100'], ['B01.050.150.900.649.313.988.400.600.150.170.120'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.350.515', 'E05.595', 'H01.671.617.562'], ['A05.360.319.114.630.535', 'A06.300.312.497.535'], ['E04.680'], ['E01.370.350.850.850.850']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Anatomy [A]']
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
Particle bombardment-mediated transient expression of a Brazil nut methionine-rich albumin in bean (Phaseolus vulgaris L.).
|
Bean (Phaseolus vulgaris L.) mature embryos were transformed using biolistic methods with a plasmid containing 2S albumin and beta-glucuronidase structural sequences, both under the control of the 35S CaMV promoter. We have shown that chimaeric tissues could be obtained and that both structural sequences were expressed to similar levels.
|
['2S Albumins, Plant', 'Antigens, Plant', 'Blotting, Western', 'Cloning, Molecular', 'Electrophoresis, Polyacrylamide Gel', 'Fabaceae', 'Nuts', 'Plant Proteins', 'Plants, Medicinal', 'Recombinant Fusion Proteins']
| 1,391,783
|
[['D12.776.765.725.249'], ['D23.050.291'], ['E05.196.401.143', 'E05.301.300.096', 'E05.478.566.320.200', 'E05.601.262', 'E05.601.470.320.200'], ['E05.393.220'], ['E05.196.401.402', 'E05.301.300.319'], ['B01.650.940.800.575.912.250.401'], ['A18.024.500.500', 'G07.203.300.700', 'J02.500.700'], ['D12.776.765'], ['B01.650.560'], ['D12.776.828.300']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Technology, Industry, and Agriculture [J]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Analysis of factors affecting failure of glass cermet tunnel restorations in a multi-center study.
|
The aim of this study was to analyze factors influencing the failures of tunnel restorations performed with a glass cermet cement (Ketac Silver). Caries activity, lesion size, tunnel cavity opening size, partial or total tunnel, composite lamination or operating time showed no significant correlation to failure rate. Twelve dentists in eight clinics clinically experienced and familiar with the tunnel technique placed 374 restorations. The occlusal sections of fifty percent of the restorations were laminated with hybrid resin composite. The results of the yearly clinical and radiographic evaluations over the course of 3 years were correlated to factors that could influence the failure rate using logistic regression analysis. At the 3-year recall a cumulative number of 305 restorations were available. The cumulative replacement rate was 20%. The main reasons for replacement were marginal ridge fracture (14%) and dentin caries (3%). Another 7% of the restorations which had not been replaced were classified as failures because of untreated dentin caries. The only significant variable observed was the individual failure rate of the participating dentists varying between 9 and 50% (p=0.013).
|
['Adolescent', 'Adult', 'Bicuspid', 'Cermet Cements', 'Child', 'Composite Resins', 'Dental Caries', 'Dental Caries Susceptibility', 'Dental Cavity Preparation', 'Dental Restoration Failure', 'Dental Restoration, Permanent', 'Dentin', 'Female', 'Follow-Up Studies', 'Humans', 'Logistic Models', 'Male', 'Molar', 'Radiography', 'Recurrence', 'Retreatment', 'Surface Properties', 'Time Factors', 'Tooth']
| 11,480,816
|
[['M01.060.057'], ['M01.060.116'], ['A14.549.167.860.150'], ['D01.552.033.690.250', 'D25.058.520.250', 'D25.339.208.720.250', 'D25.339.291.402.120', 'J01.637.051.058.520.250', 'J01.637.051.339.208.720.250', 'J01.637.051.339.291.402.120'], ['M01.060.406'], ['D05.750.716.822.308', 'D25.339.816.500', 'D25.720.716.822.308', 'J01.637.051.339.816.500', 'J01.637.051.720.716.822.308'], ['C07.793.720.210'], ['G10.549.140'], ['E06.931.325'], ['E06.323.400', 'E06.780.346.725'], ['E06.323.428', 'E06.780.346.737', 'E07.695.190.190'], ['A14.549.167.900.280'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.740.500.525', 'E05.318.740.600.800.450', 'E05.318.740.750.450', 'E05.599.835.875', 'N05.715.360.750.530.480', 'N05.715.360.750.625.700.450', 'N05.715.360.750.695.470', 'N06.850.520.830.500.525', 'N06.850.520.830.600.800.450', 'N06.850.520.830.750.450'], ['A14.549.167.860.525'], ['E01.370.350.700'], ['C23.550.291.937'], ['E02.887'], ['G02.860'], ['G01.910.857'], ['A14.549.167.860']]
|
['Named Groups [M]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Technology, Industry, and Agriculture [J]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 1
| 0
|
The boron-neutron capture agent beta-D-5-o-carboranyl-2'-deoxyuridine accumulates preferentially in dividing brain tumor cells.
|
Boron-neutron capture therapy (BNCT) is based on the preferential targeting of tumor cells with (10)B and subsequent irradiation with epithermal neutrons to produce a highly localized field of lethal alpha particles, while sparing neighboring non-targeted cells. BNCT treatment of 9L brain tumors in a rat model using beta-D-5-o-carboranyl-2'-deoxyuridine (D-CDU) resulted in greater efficacy than predicted based on the assumption of a uniform tumor distribution of (10)B. Thus, the geometric heterogeneity of dividing cells in brain tumors warranted studies on the cell cycle dependency of D-CDU accumulation, metabolism and entrapment in a relevant brain tumor cell system. U-271 human glioma cells were synchronized in G(1) or S-phases of the cell cycle. The cellular accumulation and phosphorylation of D-CDU was measured in the G(1) and S-phase cells using high-performance liquid chromatography (HPLC). Cells synchronized in the S-phase accumulated significantly higher amounts of D-CDU and produced larger amounts of negatively charged D-CDU monophosphate (D-CDU-MP) and nido-CDU metabolites than resting cells. Since brain tumors contain a larger proportion of cycling cells than neighboring tissue, these results support the hypothesis that in addition to breakdown of the blood-brain-barrier (BBB) in tumors, the preferential phosphorylation of D-CDU in cycling cells may further enrich the distribution of (10)B in dividing cells. Therefore, dosimetry calculations that include the spatial distribution of cycling cells may be warranted for D-CDU.
|
['Boron Neutron Capture Therapy', 'Brain Neoplasms', 'Cell Cycle', 'Cell Line, Tumor', 'Cell Proliferation', 'Chromatography, High Pressure Liquid', 'Deoxyuridine', 'Flow Cytometry', 'Glioma', 'Humans', 'Radiation-Sensitizing Agents']
| 16,132,519
|
[['E02.815.722.500.100'], ['C04.588.614.250.195', 'C10.228.140.211', 'C10.551.240.250'], ['G04.144'], ['A11.251.210.190', 'A11.251.860.180'], ['G04.161.750', 'G07.345.249.410.750'], ['E05.196.181.400.300'], ['D03.383.742.680.852.300', 'D13.570.230.430', 'D13.570.685.852.300'], ['E01.370.225.500.363.342', 'E01.370.225.500.386.350', 'E05.196.712.516.600.240.350', 'E05.200.500.363.342', 'E05.200.500.386.350', 'E05.242.363.342', 'E05.242.386.350'], ['C04.557.465.625.600.380', 'C04.557.470.670.380', 'C04.557.580.625.600.380'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D27.505.954.600']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Organisms [B]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Body burdens of mercury, lead, selenium and copper among Baltimore newborns.
|
Umbilical cord blood or serum concentrations of mercury, lead, selenium and copper were measured with inductively coupled plasma mass spectrometry in a population of 300 infants born in Baltimore, Maryland. Geometric mean values were 1.37 ìg/L (95% confidence interval: 1.27, 1.48) for mercury; 0.66 ìg/dL (95% CI: 0.61, 0.71) for lead; and 38.62 ìg/dL (95% CI: 36.73, 40.61) for copper. Mean selenium was 70.10 ìg/L (95% CI: 68.69, 70.52). Mercury, selenium and copper levels were within exposure ranges reported among similar populations, whereas the distribution of lead levels was lower than prior reports; only one infant had a cord blood lead above 10 ìg/dL. Levels of selenium were significantly correlated with concentrations of lead (Spearman's ñ=0.20) and copper (Spearman's ñ=0.51). Multivariable analyses identified a number of factors associated with one of more of these exposures. These included: increase in maternal age (increased lead); Asian mothers (increased mercury and lead, decreased selenium and copper); higher umbilical cord serum n-3 fatty acids (increased mercury, selenium and copper), mothers using Medicaid (increased lead); increasing gestational age (increased copper); increasing birthweight (increased selenium); older neighborhood housing stock (increased lead and selenium); and maternal smoking (increased lead). This work provides additional information about contemporary prenatal element exposures and can help identify groups at risk of atypical exposures.
|
['Adolescent', 'Adult', 'Baltimore', 'Body Burden', 'Copper', 'Cross-Sectional Studies', 'Fatty Acids, Omega-3', 'Female', 'Fetal Blood', 'Humans', 'Infant, Newborn', 'Lead', 'Maternal Exposure', 'Mercury', 'Pregnancy', 'Selenium', 'Socioeconomic Factors', 'Statistics, Nonparametric', 'Urban Population', 'Young Adult']
| 21,277,575
|
[['M01.060.057'], ['M01.060.116'], ['Z01.107.567.875.500.500.100', 'Z01.433.100'], ['E05.799.638.231', 'N06.850.460.200'], ['D01.268.556.195', 'D01.268.956.170', 'D01.552.544.195'], ['E05.318.372.500.875', 'N05.715.360.330.500.875', 'N06.850.520.450.500.875'], ['D10.212.302.380.410', 'D10.251.355.337', 'D10.627.430.450'], ['A12.207.152.200', 'A15.145.300', 'A16.378.200'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703.520'], ['D01.268.556.435', 'D01.552.544.435'], ['N06.850.460.350.145'], ['D01.268.556.504', 'D01.268.956.437', 'D01.552.544.504'], ['G08.686.784.769'], ['D01.268.185.850', 'D01.578.700'], ['I01.880.853.996', 'N01.824'], ['E05.318.740.995', 'N05.715.360.750.760', 'N06.850.520.830.995'], ['N01.600.900'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Anthropology, Education, Sociology, and Social Phenomena [I]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 1
|
Fruit flies (Diptera, Tephritidae) and their associations with native host plants in a remnant area of the highly endangered Atlantic Rain Forest in the State of Esp?rito Santo, Brazil.
|
The results presented in this paper refer to a host survey, lasting approximately three and a half years (February 2003-July 2006), undertaken in the Vale do Rio Doce Natural Reserve, a remnant area of the highly endangered Atlantic Rain Forest located in Linhares County, State of Esp?rito Santo, Brazil. A total of 330 fruit samples were collected from native plants, representing 248 species and 51 plant families. Myrtaceae was the most diverse family with 54 sampled species. Twenty-eight plant species, from ten families, are hosts of ten Anastrepha species and of Ceratitis capitata (Wiedemann). Among 33 associations between host plants and fruit flies, 20 constitute new records, including the records of host plants for A. fumipennis Lima and A. nascimentoi Zucchi. The findings were discussed in the light of their implications for rain forest conservation efforts and the study of evolutionary relationships between fruit flies and their hosts.
|
['Animals', 'Biological Evolution', 'Brazil', 'Conservation of Natural Resources', 'Feeding Behavior', 'Fruit', 'Tephritidae', 'Tropical Climate']
| 18,439,337
|
[['B01.050'], ['G05.045', 'G16.075'], ['Z01.107.757.176'], ['J01.256', 'N06.230.080'], ['F01.145.113.547', 'F01.145.407', 'G07.203.650.353'], ['A18.024.500', 'G07.203.300.562', 'J02.500.562'], ['B01.050.500.131.617.720.500.500.750.850'], ['G16.500.275.071.600', 'N06.230.300.100.250.600']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Geographicals [Z]', 'Technology, Industry, and Agriculture [J]', 'Health Care [N]', 'Psychiatry and Psychology [F]', 'Anatomy [A]']
| 1
| 1
| 0
| 0
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
|
Identifying dynamic functional connectivity biomarkers using GIG-ICA: Application to schizophrenia, schizoaffective disorder, and psychotic bipolar disorder.
|
Functional magnetic resonance imaging (fMRI) studies have shown altered brain dynamic functional connectivity (DFC) in mental disorders. Here, we aim to explore DFC across a spectrum of symptomatically-related disorders including bipolar disorder with psychosis (BPP), schizoaffective disorder (SAD), and schizophrenia (SZ). We introduce a group information guided independent component analysis procedure to estimate both group-level and subject-specific connectivity states from DFC. Using resting-state fMRI data of 238 healthy controls (HCs), 140 BPP, 132 SAD, and 113 SZ patients, we identified measures differentiating groups from the whole-brain DFC and traditional static functional connectivity (SFC), separately. Results show that DFC provided more informative measures than SFC. Diagnosis-related connectivity states were evident using DFC analysis. For the dominant state consistent across groups, we found 22 instances of hypoconnectivity (with decreasing trends from HC to BPP to SAD to SZ) mainly involving post-central, frontal, and cerebellar cortices as well as 34 examples of hyperconnectivity (with increasing trends HC through SZ) primarily involving thalamus and temporal cortices. Hypoconnectivities/hyperconnectivities also showed negative/positive correlations, respectively, with clinical symptom scores. Specifically, hypoconnectivities linking postcentral and frontal gyri were significantly negatively correlated with the PANSS positive/negative scores. For frontal connectivities, BPP resembled HC while SAD and SZ were more similar. Three connectivities involving the left cerebellar crus differentiated SZ from other groups and one connection linking frontal and fusiform cortices showed a SAD-unique change. In summary, our method is promising for assessing DFC and may yield imaging biomarkers for quantifying the dimension of psychosis. Hum Brain Mapp 38:2683-2708, 2017. © 2017 Wiley Periodicals, Inc.
|
['Adult', 'Bipolar Disorder', 'Brain', 'Brain Mapping', 'Female', 'Humans', 'Image Processing, Computer-Assisted', 'Magnetic Resonance Imaging', 'Male', 'Middle Aged', 'Nonlinear Dynamics', 'Oxygen', 'Principal Component Analysis', 'Psychotic Disorders', 'Schizophrenia', 'Time Factors', 'Young Adult']
| 28,294,459
|
[['M01.060.116'], ['F03.084.500'], ['A08.186.211'], ['E01.370.350.578.875.500', 'E01.370.376.537.625.500', 'E05.629.875.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['L01.224.308'], ['E01.370.350.825.500'], ['M01.060.116.630'], ['E05.599.850', 'H01.548.675'], ['D01.268.185.550', 'D01.362.670'], ['E05.318.740.562'], ['F03.700.675'], ['F03.700.750'], ['G01.910.857'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Psychiatry and Psychology [F]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Information Science [L]', 'Disciplines and Occupations [H]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 1
| 0
| 0
|
The Impact of Plasmacytoid Variant Histology on the Survival of Patients with Urothelial Carcinoma of Bladder after Radical Cystectomy.
|
BACKGROUND: The clinical significance of the plasmacytoid variant (PCV) in urothelial carcinoma (UC) is currently lacking.OBJECTIVE: To compare clinical outcomes of patients with any PCV with that of patients with pure UC treated with radical cystectomy (RC).DESIGN, SETTING, AND PARTICIPANTS: We identified 98 patients who had pathologically confirmed PCV UC and 1312 patients with pure UC and no variant history who underwent RC at our institution between 1995 and 2014.OUTCOME MEASUREMENTS AND STATISTICAL ANALYSIS: Univariable and multivariable Cox regression and Cox proportional hazards regression to determine if PCV was associated with overall survival (OS).RESULTS AND LIMITATIONS: Patients with PCV UC were more likely to have advanced tumor stage (p=0.001), positive lymph nodes (p=0.038), and receive neoadjuvant chemotherapy than those with pure UC (46% vs 22%, p<0.0001). The rate of positive soft tissue surgical margins was over five times greater in the PCV UC group compared with the pure UC group (21% vs 4.1%, respectively, p<0.0001). Median OS for the pure UC versus the PCV patients were 8 yr and 3.8 yr, respectively. On univariable analysis, PCV was associated with an increased risk of overall mortality (hazard ratio=1.34, 95% confidence interval: 1.02-1.78, p=0.039). However, on multivariable analysis adjusted for age, sex, neoadjuvant chemotherapy received, lymph node status, pathologic stage, and soft margin status, the association between PCV and OS was no longer significant (hazard ratio=1.06, 95% confidence interval: 0.78, 1.43, p=0.7). This retrospective study is limited by the lack of pathological reanalysis, and the impact of other concurrent mixed histology cannot be determined in this study.CONCLUSIONS: Patients with PCV features have a higher disease burden at RC compared with those with pure UC. However, PCV was not an independent predictor of survival after RC on multivariable analysis, suggesting that PCV histology should not be used as an independent prognostic factor.PATIENT SUMMARY: Plasmacytoid urothelial carcinoma is a rare and aggressive form of bladder cancer. Patients with plasmacytoid urothelial carcinoma had worse adverse pathologic features, but this was not associated with worse overall mortality when compared with patients with pure urothelial carcinoma.
|
['Aged', 'Carcinoma, Transitional Cell', 'Cystectomy', 'Female', 'Humans', 'Kaplan-Meier Estimate', 'Male', 'Middle Aged', 'Proportional Hazards Models', 'Retrospective Studies', 'Survival Rate', 'Treatment Outcome', 'Urinary Bladder Neoplasms']
| 28,753,857
|
[['M01.060.116.100'], ['C04.557.470.200.430'], ['E04.950.774.150'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.740.998.650', 'N05.715.360.750.795.650', 'N06.850.520.830.998.650'], ['M01.060.116.630'], ['E05.318.740.500.700', 'E05.318.740.600.700', 'E05.318.740.750.725', 'E05.318.740.998.825', 'E05.599.835.900', 'N05.715.360.750.530.650', 'N05.715.360.750.625.650', 'N05.715.360.750.695.650', 'N05.715.360.750.795.825', 'N06.850.520.830.500.700', 'N06.850.520.830.600.700', 'N06.850.520.830.750.725', 'N06.850.520.830.998.912'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E05.318.308.985.550.900', 'N01.224.935.698.826', 'N06.850.505.400.975.550.900', 'N06.850.520.308.985.550.900'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['C04.588.945.947.960', 'C12.758.820.968', 'C12.777.829.813', 'C13.351.937.820.945', 'C13.351.968.829.707']]
|
['Named Groups [M]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Health Care [N]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
SSWAP: A Simple Semantic Web Architecture and Protocol for semantic web services.
|
BACKGROUND: SSWAP (Simple Semantic Web Architecture and Protocol; pronounced "swap") is an architecture, protocol, and platform for using reasoning to semantically integrate heterogeneous disparate data and services on the web. SSWAP was developed as a hybrid semantic web services technology to overcome limitations found in both pure web service technologies and pure semantic web technologies.RESULTS: There are currently over 2400 resources published in SSWAP. Approximately two dozen are custom-written services for QTL (Quantitative Trait Loci) and mapping data for legumes and grasses (grains). The remaining are wrappers to Nucleic Acids Research Database and Web Server entries. As an architecture, SSWAP establishes how clients (users of data, services, and ontologies), providers (suppliers of data, services, and ontologies), and discovery servers (semantic search engines) interact to allow for the description, querying, discovery, invocation, and response of semantic web services. As a protocol, SSWAP provides the vocabulary and semantics to allow clients, providers, and discovery servers to engage in semantic web services. The protocol is based on the W3C-sanctioned first-order description logic language OWL DL. As an open source platform, a discovery server running at http://sswap.info (as in to "swap info") uses the description logic reasoner Pellet to integrate semantic resources. The platform hosts an interactive guide to the protocol at http://sswap.info/protocol.jsp, developer tools at http://sswap.info/developer.jsp, and a portal to third-party ontologies at http://sswapmeet.sswap.info (a "swap meet").CONCLUSION: SSWAP addresses the three basic requirements of a semantic web services architecture (i.e., a common syntax, shared semantic, and semantic discovery) while addressing three technology limitations common in distributed service systems: i.e., i) the fatal mutability of traditional interfaces, ii) the rigidity and fragility of static subsumption hierarchies, and iii) the confounding of content, structure, and presentation. SSWAP is novel by establishing the concept of a canonical yet mutable OWL DL graph that allows data and service providers to describe their resources, to allow discovery servers to offer semantically rich search engines, to allow clients to discover and invoke those resources, and to allow providers to respond with semantically tagged data. SSWAP allows for a mix-and-match of terms from both new and legacy third-party ontologies in these graphs.
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['Computational Biology', 'Databases, Factual', 'Information Dissemination', 'Information Storage and Retrieval', 'Internet', 'Semantics', 'Software', 'User-Computer Interface']
| 19,775,460
|
[['H01.158.273.180', 'L01.313.124'], ['L01.313.500.750.300.188.400', 'L01.470.750.750'], ['L01.143.443'], ['L01.313.500.750.280', 'L01.470'], ['L01.224.230.110.500'], ['L01.559.598.745'], ['L01.224.900'], ['L01.224.900.910']]
|
['Disciplines and Occupations [H]', 'Information Science [L]']
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
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Hydrazone- and hydrazide-containing N-substituted glycines as peptoid surrogates for expedited library synthesis: application to the preparation of Tsg101-directed HIV-1 budding antagonists.
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Replacing the Pro6 in the p6(Gag)-derived 9-mer "P-E-P-T-A-P-P-E-E" with N-substituted glycine (NSG) residues is problematic. However, incorporation of hydrazone amides ("peptoid hydrazones") can be readily achieved in library fashion. Furthermore, reduction of these hydrazones to N-substituted "peptoid hydrazides" affords a facile route to library diversification. This approach is demonstrated by application to Tsg101-binding compounds designed as potential HIV budding antagonists. [reaction: see text]
|
['Amino Acid Sequence', 'Anti-HIV Agents', 'DNA-Binding Proteins', 'Endosomal Sorting Complexes Required for Transport', 'Glycine', 'HIV-1', 'Hydrazines', 'Hydrazones', 'Oligopeptides', 'Peptide Library', 'Peptoids', 'Proline', 'Transcription Factors']
| 17,048,869
|
[['G02.111.570.060', 'L01.453.245.667.060'], ['D27.505.954.122.388.077.088'], ['D12.776.260'], ['D05.500.199', 'D12.776.543.990.493'], ['D12.125.481'], ['B04.820.650.589.650.350.400'], ['D02.442'], ['D02.442.288'], ['D12.644.456'], ['D12.644.555', 'G02.111.570.060.620', 'G05.360.325.640'], ['D12.644.679'], ['D12.125.072.401.623'], ['D12.776.930']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Chemicals and Drugs [D]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
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An oral administration of a recombinant anti-TNF fusion protein is biologically active in the gut promoting regulatory T cells: Results of a phase I clinical trial using a novel oral anti-TNF alpha-based therapy.
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BACKGROUND: An orally administered BY-2 plant cell-expressed recombinant anti-TNF fusion protein (PRX-106) consists of the soluble form of the human TNF receptor (TNFR) fused to the Fc component of a human IgG1 domain. Aim This study aim at determining the safety and the immune modulatory effect of an oral administration of PRX-106 in humans.METHODS: Three different doses (2, 8 or 16mg/day) of PRX-106 were orally administered for five consecutive days in 14 healthy volunteered participants. Subjects were followed for safety parameters and for an effect on T lymphocytes subsets and cytokine levels.RESULTS: An oral administration of PRX-106 was safe and well tolerated. The PK study showed that PRX106 is not absorbed. No effect on white blood cells and lymphocytes counts were noted. A dose dependent effect was noted on systemic lymphocytes. The oral administration of all three dosages was associated with an increase in CD4+CD25+ and CD8+CD25+ subset of suppressor lymphocytes. A marked increase in CD4+CD25+FoxP3 regulatory T cells was noted in the 8mg treated group. In addition, NKT regulatory cells, CD3+CD69+ and CD4+CD62 lymphocyte subsets increased with treatment. No changes in serum TNF alpha were observed.CONCLUSION: An oral administration of the non-absorbable recombinant anti-TNF fusion protein, PRX-106, is safe, not associated with immune suppression, while inducing a favorable anti-inflammatory immune modulation. The PRX-106 may provide a safe orally administered effective anti-TNF alpha-based immune therapy for inflammatory bowel diseases and non-alcoholic steatohepatitis, as well as other autoimmune, TNF-mediated diseases.
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['Administration, Oral', 'Adolescent', 'Adult', 'Cytokines', 'Etanercept', 'Humans', 'Immunomodulation', 'Immunotherapy', 'Inflammatory Bowel Diseases', 'Lymphocyte Activation', 'Male', 'Middle Aged', 'T-Lymphocytes, Regulatory', 'Tumor Necrosis Factor-alpha', 'Young Adult']
| 28,392,436
|
[['E02.319.267.100'], ['M01.060.057'], ['M01.060.116'], ['D12.644.276.374', 'D12.776.467.374', 'D23.529.374'], ['D12.644.541.500.697.624', 'D12.776.124.486.485.538.500.624', 'D12.776.124.486.485.680.697.624', 'D12.776.124.790.651.538.500.624', 'D12.776.124.790.651.680.660.624', 'D12.776.377.715.548.538.500.624', 'D12.776.377.715.548.680.660.624', 'D12.776.543.750.705.852.760.232'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.095.465', 'G12.535'], ['E02.095.465.425'], ['C06.405.205.731', 'C06.405.469.432'], ['E01.370.225.812.482', 'E05.200.812.482', 'E05.478.594.530', 'G12.450.050.400.545', 'G12.565'], ['M01.060.116.630'], ['A11.118.637.555.567.550.500.700', 'A11.118.637.555.567.569.200.700', 'A11.118.637.555.567.569.500.700', 'A15.145.229.637.555.567.550.500.700', 'A15.145.229.637.555.567.569.200.700', 'A15.145.229.637.555.567.569.500.700', 'A15.382.490.555.567.550.500.700', 'A15.382.490.555.567.569.200.700', 'A15.382.490.555.567.569.500.700'], ['D12.644.276.374.500.800', 'D12.644.276.374.750.626', 'D12.776.124.900', 'D12.776.395.930', 'D12.776.467.374.500.800', 'D12.776.467.374.750.626', 'D23.529.374.500.800', 'D23.529.374.750.626'], ['M01.060.116.815']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Named Groups [M]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
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Photothermal studies of CO photodissociation from mixed valence Escherichia coli cytochrome bo3.
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Here, we report the volume and enthalpy changes accompanying CO photodissociation from the mixed valence form of cytochrome bo3 oxidase from Escherichia coli. The results of photoacoustic calorimetry indicate two kinetic phases with distinct volume and enthalpy changes accompanying CO photodissociation from heme o3 and its transfer to CuB. The first phase occurring on a timescale of <50 ns is characterized by a volume decrease of -1.3+/-0.3 mL mol-1 and enthalpy change of 32+/-1.6 kcal mol-1. Subsequently, a volume increase of 2.9 mL mol-1 with an enthalpy change of -5.3+/-2.5 kcal mol-1 is observed with the lifetime of approximately 250 ns (this phase has not been detected in previous optical studies). These volume and enthalpy changes differ from the volume and enthalpy changes observed for CO dissociation from fully reduced cytochrome bo3 oxidase indicating that the heme o3/CuB active site dynamics are affected by the redox state of heme b.
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['Carbon Monoxide', 'Cytochrome b Group', 'Cytochromes', 'Escherichia coli', 'Escherichia coli Proteins', 'Kinetics', 'Ligands', 'Molecular Conformation', 'Oxidation-Reduction', 'Photolysis', 'Spectroscopy, Fourier Transform Infrared', 'Temperature', 'Thermodynamics']
| 15,896,790
|
[['D01.200.250', 'D01.362.200', 'D01.650.550.250'], ['D08.244.187', 'D12.776.422.220.187'], ['D08.244', 'D12.776.422.220'], ['B03.440.450.425.325.300', 'B03.660.250.150.180.100'], ['D12.776.097.275'], ['G01.374.661', 'G02.111.490'], ['D27.720.470.480'], ['G02.111.570.820'], ['G02.700', 'G03.295.531'], ['G02.740.685'], ['E05.196.712.726.676.700', 'E05.196.867.826.676.700'], ['G01.906.595', 'G16.500.275.063.725.710', 'G16.500.750.775.710', 'N06.230.150.450', 'N06.230.300.100.725.710'], ['G01.906']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
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Pre-procedural bioimpedance vectorial analysis of fluid status and prediction of contrast-induced acute kidney injury.
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OBJECTIVES: The aim of this study was to evaluate the relationship between pre-procedural fluid status assessed by bioimpedance vector analysis (BIVA) and development of contrast-induced acute kidney injury (CI-AKI).BACKGROUND: Accurate fluid management in patients undergoing angiographic procedures is of critical importance in limiting the risk of CI-AKI. Therefore, establishing peri-procedural fluid volume related to increased risk of CI-AKI development is essential.METHODS: We evaluated the fluid status by BIVA of 900 consecutive patients with stable coronary artery disease (CAD) immediately before coronary angiography, measuring the resistance/height (R/H) ratio and impedance/height (Z/H) vector. CI-AKI was defined as an increase in serum creatinine ?0.5 mg/dl above baseline within 3 days after contrast administration (iodixanol).RESULTS: CI-AKI occurred in 54 patients (6.0%). Pre-procedural R/H ratios were significantly higher in patients with CI-AKI than without CI-AKI (395 ± 71 Ohm/m vs. 352 ± 58 Ohm/m, p = 0.001 for women; 303 ± 59 Ohm/m vs. 279 ± 45 Ohm/m, p = 0.009 for men), indicating lower fluid volume in the patients with CI-AKI. When patients were stratified according to R/H ratio, there was an almost 3-fold higher risk in patients with higher values (odds ratio [OR]: 2.9; 95% confidence interval [CI]: 1.5 to 5.5; p = 0.002). The optimal receiver-operating characteristic curve analysis threshold values of R/H ratio for predicting CI-AKI were 380 Ohm/m for women and 315 Ohm/m for men. R/H ratio above these thresholds was found to be a significant and independent predictor of CI-AKI (OR: 3.1; 95% CI: 1.8 to 5.5; p = 0.001).CONCLUSIONS: Lower fluid status evaluated by BIVA immediately before contrast medium administration resulted in a significant and independent predictor of CI-AKI in patients with stable CAD. This simple noninvasive analysis should be tested in guiding tailored volume repletion.
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['Acute Kidney Injury', 'Aged', 'Cohort Studies', 'Contrast Media', 'Coronary Angiography', 'Coronary Artery Disease', 'Electric Impedance', 'Female', 'Humans', 'Hydrodynamics', 'Male', 'Middle Aged', 'Odds Ratio', 'Retrospective Studies', 'Triiodobenzoic Acids', 'Water-Electrolyte Imbalance']
| 24,530,668
|
[['C12.777.419.780.050', 'C13.351.968.419.780.050'], ['M01.060.116.100'], ['E05.318.372.500.750', 'N05.715.360.330.500.750', 'N06.850.520.450.500.750'], ['D27.505.259.500', 'D27.720.259'], ['E01.370.350.130.625', 'E01.370.350.700.060.200', 'E01.370.370.050.200', 'E01.370.370.380.200'], ['C14.280.647.250.260', 'C14.907.137.126.339', 'C14.907.585.250.260'], ['G01.358.500.249.277.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G01.342'], ['M01.060.116.630'], ['E05.318.740.600.600', 'G17.680.500', 'N05.715.360.750.625.590', 'N06.850.520.830.600.600'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['D02.241.223.100.400.880', 'D02.455.426.559.389.127.375.880'], ['C18.452.950']]
|
['Diseases [C]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 1
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| 1
| 0
| 0
| 0
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| 1
| 1
| 0
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In vitro reendothelialization. Microfilament bundle reorganization in migrating porcine endothelial cells.
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An experimentally induced wound made in a confluent monolayer culture of porcine thoracic aortic endothelial cells (ECs) was studied 22 hours after wounding using 7-nitrobenz-2-oxa-1,3-diazole (NBD) phallacidin and immunofluorescence microscopy to localize actin and myosin containing microfilament (MF) bundles. ECs extending from the wound edge back toward the confluent monolayer showed a specific change in cell shape and in MF bundle distribution and orientation, which correlated with the cell migration behavior observed using time-lapse cinemicrophotography. The migrating ECs in the first zone, the leading zone, were polygonal to partially elongated in shape, and contained distinct central MF bundles oriented both parallel and perpendicular to the wound edge. The second zone, the elongated zone, was characterized by elongated cells with central MF bundles oriented parallel to the direction of migration. A third zone, the transitional zone, showed nonmigrating polygonal ECs containing prominent central and dense peripheral bands (DPB) of MF bundles. The central MF bundles were oriented randomly with respect to the wound edge. The MF bundles of the confluent resting monolayer were both centrally and peripherally located with the latter being more prominent. The results indicate that the reorientation of central MF bundles and reduction in the peripheral MF bundles are probably important in the reorganization of the cytoskeletal system during the conversion of stationary cells to migrating cells.
|
['Actins', 'Amanitins', 'Animals', 'Aorta, Thoracic', 'Cell Movement', 'Cytoskeleton', 'Endothelium', 'Fluorescent Antibody Technique', 'Myosins', 'Swine', 'Time Factors', 'Wound Healing']
| 6,367,729
|
[['D05.750.078.730.250', 'D12.776.210.500.100', 'D12.776.220.525.255'], ['D04.345.566.050', 'D12.644.456.050', 'D12.644.641.050', 'D23.946.587.175'], ['B01.050'], ['A07.015.114.056.372'], ['G04.198', 'G07.568.500.180'], ['A11.284.430.214.190.750'], ['A10.272.491'], ['E01.370.225.500.607.512.240', 'E01.370.225.750.551.512.240', 'E05.200.500.607.512.240', 'E05.200.750.551.512.240', 'E05.478.583.375'], ['D05.750.078.730.475', 'D08.811.277.040.025.193.750', 'D12.776.210.500.600', 'D12.776.220.525.475'], ['B01.050.150.900.649.313.500.880'], ['G01.910.857'], ['G16.762.891']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
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[Craniofacial augmentation with porous polyethylene implants (Medpor: first clinical results].
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SUBJECT: Porous polyethylene (Medpor) is an alloplastic material commonly used in craniofacial reconstruction. We report about our first clinical experiences with Medpor for facial augmentation procedures.PATIENTS AND METHODS: We treated 27 patients between 2001 and 2005 (11 female, 16 male) with 48 Medpor-implants. The indications for application of porous polyethylene implants in our clinic were congenital malformations (15), post-traumatic defects (10), and reconstructions after tumor resection (2). The implants were used for nasal/paranasal augmentations (16), for zygomatico-orbital augmentations (18), and for augmentations of the chin and malar region (11). The procedures were performed in a standardized manner. We used prefabricated, self-contoured implants and fixed them subperiosteally with titanium osteosynthesis screws. All operations were performed under general anesthesia. We evaluated the aesthetic results and the ingrowth behavior clinically and histologically.RESULTS: We achieved good aesthetic results and the patients showed no signs of discomfort or rejection. Four patients required a second intervention. These revision surgeries included two cases of local infections and two for aesthetic contouring. The necessary reduction of the implants allowed the harvesting of tissue and implant samples for microscopy.CONCLUSION: Porous polyethylene implants showed a good fibrovascular integration without encapsulation under the light microscope. Giant cells were detected on the surface of the implants. Besides this there was evidence for resorption of the implant material. Fixation with titanium screws is very effective. No implant dislocation or implant fracture occurred. The implants showed high volume stability and were easily handled and contoured. It is not possible to visualize Medpor implants with current imaging techniques, because polyethylene shows no contrast.
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['Adolescent', 'Adult', 'Biocompatible Materials', 'Bone Screws', 'Child', 'Craniofacial Abnormalities', 'Device Removal', 'Esthetics', 'Facial Injuries', 'Facial Neoplasms', 'Female', 'Follow-Up Studies', 'Humans', 'Male', 'Polyethylenes', 'Prostheses and Implants', 'Prosthesis Design', 'Prosthesis-Related Infections', 'Reoperation', 'Surface Properties']
| 16,685,567
|
[['M01.060.057'], ['M01.060.116'], ['D25.130', 'D27.720.102.130', 'J01.637.051.130'], ['E07.695.370.437', 'E07.858.442.660.460.437', 'E07.858.690.725.460.437'], ['M01.060.406'], ['C05.660.207', 'C16.131.621.207'], ['E04.199'], ['F02.463.785.477', 'K01.752.210'], ['C10.900.300.284', 'C26.915.300.425'], ['C04.588.443.392'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D02.455.326.271.665.550', 'D05.750.716.507', 'D25.720.716.507', 'J01.637.051.720.716.507'], ['E07.695'], ['E05.320.550', 'E07.695.680'], ['C01.685', 'C23.550.767.868'], ['E04.690'], ['G02.860']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Technology, Industry, and Agriculture [J]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Humanities [K]', 'Health Care [N]', 'Organisms [B]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 1
| 0
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Negative and helpful statements in a patient only or therapist guided internet forum in the aftercare for psychosomatic inpatients.
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BACKGROUND: The aftercare of patients in the wake of inpatient treatment is a therapeutic challenge. Aim of the present study was to investigate the utilization, and positive or negative interactions in a patient only (PF) and a therapist guided internet forum (TF) in the follow up of psychosomatic inpatient treatment.SUBJECTS AND METHODS: After discharge from hospital, patients were offered to participate in a PF or TF. The rate and duration of participation was monitored and the content of communication classified by content analysis.RESULTS: In the PF 144 (8.0%) and in TF 76 (8.5%) of invited patients registered. Participants were somewhat younger, more often male, and professionally better qualified. Time of participation was 51 (sd 90) days in the PF and 59 (sd 69) in the TF. 32% of the registered patients wrote messages in the PF and 43% in the TF, with 7 (sd 10) comments on average in the PF and 15 (sd 26) in the TF. Most comments were health related and positive, with a higher rate in the TF. There were still 8 destructive comments in both forums.CONCLUSIONS: The rate of participation in the aftercare internet forum is low. Therapeutic guidance increases the rate of patient contributions. Most comments are supportive, but negative interactions occur even in the presence of a moderator.
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['Adult', 'Aftercare', 'Female', 'Helping Behavior', 'Humans', 'Internet', 'Male', 'Middle Aged', 'Patient Discharge', 'Pessimism', 'Physician-Patient Relations', 'Psychophysiologic Disorders', 'Psychotherapy, Group', 'Self Care', 'Therapy, Computer-Assisted']
| 29,197,201
|
[['M01.060.116'], ['E02.760.169.063', 'N02.421.585.169.063', 'N04.590.233.727.210.063'], ['F01.145.813.225'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['L01.224.230.110.500'], ['M01.060.116.630'], ['E02.760.169.125', 'E02.760.400.610', 'N02.421.585.169.125', 'N02.421.585.400.610', 'N04.590.233.727.210.125'], ['F01.100.893'], ['F01.829.401.650.675', 'N05.300.660.625'], ['C23.888.592.700'], ['F04.754.864.581'], ['E02.900', 'I03.050.563', 'N02.421.784.680'], ['E02.950', 'L01.313.500.750.100.710']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Psychiatry and Psychology [F]', 'Organisms [B]', 'Information Science [L]', 'Diseases [C]', 'Anthropology, Education, Sociology, and Social Phenomena [I]']
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 1
| 1
| 0
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Simultaneous patellar tendon avulsion fracture from both patella and tibial tuberosity: a case report.
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We present a simultaneous patella tendon avulsion fracture from both sides of its attachment in an ectopic ossified patellar tendon. Medially two thirds of the patellar tendon was detached from the inferior patella, and laterally one third of that from anterior tibial tuberosity with the patellar tendon split. Osteosynthesis was used to restore the extensor mechanism of the knee. The result at 46 months was good, even though there were some remaining ectopic ossifications in the patellar tendon.
|
['Aged', 'Humans', 'Knee Injuries', 'Male', 'Orthopedic Procedures', 'Ossification, Heterotopic', 'Patellar Ligament', 'Radiography']
| 16,897,070
|
[['M01.060.116.100'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C26.558.554'], ['E02.718', 'E04.555'], ['C23.550.751'], ['A02.513.514.475', 'A02.835.583.512.475', 'A02.880.438', 'A10.165.669.514.475'], ['E01.370.350.700']]
|
['Named Groups [M]', 'Organisms [B]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
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Angiogenesis and vascular endothelial growth factor-/receptor expression in myeloproliferative neoplasms: correlation with clinical parameters and JAK2-V617F mutational status.
|
Data on angiogenesis in the bone marrow of BCR-ABL1-negative myeloproliferative neoplasm (MPN) patients suggest an increase of the microvessel density (MVD) and vascular endothelial growth factor (VEGF) expression, but relations to the JAK2-V617F status remain controversial. We performed immunohistochemical studies of MVD and VEGF-expression in 100 MPN, including 24 essential thrombocythemia- (ET), 46 polycythemia vera- (PV), 26 primary myelofibrosis- (PMF), four myelodysplastic (MDS)/MPN- and 20 control reactive bone marrow cases, and correlated these findings with biological and clinical key data and the JAK2-V617F status. We found significantly increased MVD, particularly that assessed by CD105, and VEGF expression in MPN compared to controls (PMF > PV > MDS/MPN > ET). We observed stronger association between CD105-MVD and VEGF expression, fibrosis, and JAK2-V617F mutant allele burden, compared to CD34-MVD. MVD was strongly increased in MPN with high JAK2-V617F mutant allele burden. Our study highlights the importance of newly formed CD105+ vessels in the bone marrow of MPN patients, and indicates that assessment of CD105-MVD better reflects angiogenic activity in MPN. In addition, it provides evidence that despite the fact that angiogenesis is generally independent of the JAK2-V617F status in MPN, new vessel formation might be linked to Jak2 effects in some cases with high JAK2-V617F mutant allele burden.
|
['Bone Marrow', 'Case-Control Studies', 'Female', 'Humans', 'Immunohistochemistry', 'Janus Kinase 2', 'Male', 'Microvessels', 'Middle Aged', 'Mutation', 'Myeloproliferative Disorders', 'Neovascularization, Pathologic', 'Receptors, Vascular Endothelial Growth Factor', 'Vascular Endothelial Growth Factor A']
| 19,466,975
|
[['A15.382.216'], ['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.225.500.607.512', 'E01.370.225.750.551.512', 'E05.200.500.607.512', 'E05.200.750.551.512', 'E05.478.583', 'H01.158.100.656.234.512', 'H01.158.201.344.512', 'H01.158.201.486.512', 'H01.181.122.573.512', 'H01.181.122.605.512'], ['D08.811.913.696.620.682.725.124.200', 'D12.776.476.393.200', 'D12.776.624.664.700.117'], ['A07.015.461'], ['M01.060.116.630'], ['G05.365.590'], ['C15.378.190.636'], ['C23.550.589.500'], ['D08.811.913.696.620.682.725.400.950', 'D12.776.543.750.630.750', 'D12.776.543.750.750.400.910'], ['D12.644.276.100.800.200', 'D12.776.467.100.800.200', 'D23.529.100.800.200']]
|
['Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Disciplines and Occupations [H]', 'Chemicals and Drugs [D]', 'Named Groups [M]', 'Phenomena and Processes [G]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 1
| 1
| 0
|
Laboratory reactivity models as predictors of ambulatory blood pressure and heart rate.
|
We attempted to validate the usefulness of the laboratory reactivity paradigm by examining responses to three distinct lab tasks and an overall lab average as predictors for ambulatory blood pressure. Subjects were 126 university students. We assessed ambulatory blood pressure, heart rate and perceived distress during an 8-hr working day. In the lab, all subjects responded to three standardized 5-min tasks: isometric handgrip, mental arithmetic, and a structured discussion of a recent interpersonal conflict situation. The analyses used absolute task levels, change scores, a composite index of reactivity, and a mean value for all obtained lab measures. Task levels correlated significantly with means of the ambulatory cardiovascular indices; the scores ranged from 0.35 to 0.49 for SBP, from 0.45 to 0.58 for DBP, and from 0.57 to 0.65 for HR. Only the DBP change score associated with the discussion task correlated with the ambulatory mean. The correlations between lab task levels and ambulatory measures were strongest for the discussion task and the composite score; the task level for the math task was consistently the weakest predictor of ambulatory activity. When lab resting values were forced into a step-wise multiple regression as step one, only the response to the discussion added significantly (3% each for SBP and DBP) to the variance in ambulatory pressure that was not already explained by resting values. The overall lab mean for SBP was a better predictor of the ambulatory mean than was any combination of resting values and reactivity indices.
|
['Adolescent', 'Adult', 'Blood Pressure', 'Blood Pressure Determination', 'Female', 'Heart Rate', 'Humans', 'Laboratories', 'Male', 'Monitoring, Physiologic', 'Sex Factors', 'Stress, Psychological', 'Task Performance and Analysis']
| 8,027,961
|
[['M01.060.057'], ['M01.060.116'], ['E01.370.600.875.249', 'G09.330.380.076'], ['E01.370.370.140', 'E01.370.600.100'], ['E01.370.600.875.500', 'G09.330.380.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['J03.520', 'N02.278.487'], ['E01.370.520'], ['N05.715.350.675', 'N06.850.490.875'], ['F01.145.126.990', 'F02.830.900'], ['F02.784.412.846', 'F02.784.692.746', 'F02.808.600']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Technology, Industry, and Agriculture [J]', 'Health Care [N]', 'Psychiatry and Psychology [F]']
| 0
| 1
| 0
| 0
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 1
| 0
|
Surgery for diverticulitis in renal failure.
|
Twenty-five patients were operated on at the Brigham and Women's Hospital for colonic diverticulitis complicating treated renal failure during the period 1951 to 1983. Twelve patients had functioning renal allografts (eight cadaver, four living-related); 13 were on dialysis therapy. Six patients had polycystic kidney disease. The majority of patients had acute abdominal pain. Four had histories of chronic abdominal pain; nondiagnostic exploratory laparotomies were performed on two of these patients, who developed localized tenderness. The overall mortality in this series was 28 percent, with sepsis being the most common cause of death. Six of seven patients who died had free colonic perforations at surgery. Mortality correlated with age, with six of 14 patients (43 percent) over age 50 dying, as compared with one of 11 patients (9 percent) under age 50. There was no correlation between survival rate and type of surgery performed, dose of prednisone or azathioprine used, or type of treatment received for renal failure.
|
['Aging', 'Diverticulitis, Colonic', 'Female', 'Humans', 'Kidney Failure, Chronic', 'Kidney Transplantation', 'Male', 'Middle Aged', 'Postoperative Complications', 'Renal Dialysis', 'Retrospective Studies', 'Time Factors']
| 3,902,414
|
[['G07.345.124'], ['C06.405.205.282.500.250', 'C06.405.469.158.587.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C12.777.419.780.750.500', 'C13.351.968.419.780.750.500'], ['E02.870.500', 'E04.936.450.485', 'E04.950.774.400'], ['M01.060.116.630'], ['C23.550.767'], ['E02.870.300', 'E02.912.800'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['G01.910.857']]
|
['Phenomena and Processes [G]', 'Diseases [C]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Named Groups [M]', 'Health Care [N]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Daclatasvir plus asunaprevir for HCV genotype 1b infection in patients with or without compensated cirrhosis: a pooled analysis.
|
BACKGROUND & AIMS: We compared outcomes by cirrhosis status across studies of the all-oral combination of daclatasvir (DCV) plus asunaprevir (ASV).METHODS: Outcomes from global and Japanese phase 2 and 3 clinical studies of DCV+ASV in patients with genotype (GT) 1b infection were assessed by cirrhosis status. Sustained virological response (SVR) was assessed in individual phase 3 studies; a pooled analysis was carried out for safety outcomes.RESULTS: In the Japanese phase 3 study, SVR12 was achieved by 91% of patients with cirrhosis (n = 22) and 84% of patients without cirrhosis (n = 200); in the global phase 3 study, SVR12 was achieved by 84% of patients with cirrhosis (n = 206) and by 85% of patients without cirrhosis (n = 437). The frequency of serious adverse events, adverse events leading to treatment discontinuation and treatment-emergent grade 3/4 laboratory abnormalities was low (<10%) and similar among patients with (n = 229) or without (n = 689) compensated cirrhosis receiving DCV+ASV. Grade 3/4 reductions in platelets and neutrophils were more common among patients with cirrhosis (1.3 and 2.2%, respectively) compared with those without cirrhosis (both 0.6%). Grade 3/4 liver function test abnormalities were less common among patients with cirrhosis (1.8%) compared with those without cirrhosis (3.5-4.7%). Alanine aminotransferase elevations were not associated with hepatic decompensation.CONCLUSIONS: The safety and efficacy of DCV+ASV were similar in patients with or without compensated cirrhosis. This all-oral, interferon- and ribavirin-free combination is an effective and well-tolerated treatment option for patients with HCV GT1b infection and cirrhosis. Trial registrations numbers: Clinicaltrials.gov identifiers: NCT01012895; NCT01051414; NCT01581203; NCT01497834.
|
['Alanine Transaminase', 'Antiviral Agents', 'Drug Administration Schedule', 'Drug Therapy, Combination', 'Female', 'Hepacivirus', 'Hepatitis C, Chronic', 'Humans', 'Imidazoles', 'International Cooperation', 'Isoquinolines', 'Liver', 'Liver Cirrhosis', 'Male', 'Middle Aged', 'Sulfonamides', 'Sustained Virologic Response']
| 26,683,763
|
[['D08.811.913.477.700.100'], ['D27.505.954.122.388'], ['E02.319.283'], ['E02.319.310'], ['B04.450.380', 'B04.820.578.344.475'], ['C01.221.250.750.120', 'C01.925.440.440.120', 'C01.925.782.350.350.120', 'C06.552.380.350.120', 'C06.552.380.705.440.120'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D03.383.129.308'], ['I01.615.500'], ['D03.633.100.531'], ['A03.620'], ['C06.552.630', 'C23.550.355.412'], ['M01.060.116.630'], ['D02.065.884', 'D02.886.590.700'], ['E01.789.800.570', 'N04.761.559.590.800.665', 'N05.715.360.575.575.800.665']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Diseases [C]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Anatomy [A]', 'Named Groups [M]', 'Health Care [N]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 0
|
The relationship between basketball shooting kinematics, distance and playing position.
|
Three-dimensional cinematography (100 Hz) was used to establish the relationship between distance and the kinematics of shooting with respect to playing position in basketball. Fifteen subjects, divided into guards, forwards and centres (all n = 5), performed jump shots from each of three distances: 2.74, 4.57 and 6.40 m from the basket. Increases in mean release speed were found as shooting distance increased for all groups. This was due to increased angular velocities of both shoulder flexion and elbow extension and an increased speed of the centre of mass in the direction of the basket. Release angles for the two shorter distances (52-55 degrees) tended to provide the advantage of a steep angle of entry into the basket, whereas those at the longest distance (48-50 degrees) were closer to those requiring the minimum possible release speed. All groups exhibited an earlier timing of release as shooting distance increased, which gave rise to an earlier rotation of the shoulder axis. The more consistent changes in kinematic patterns with changes in shooting distance exhibited by guards as compared to centres would suggest that such adjustments are easier to make for those players who regularly shoot from long range.
|
['Adult', 'Analysis of Variance', 'Basketball', 'Biomechanical Phenomena', 'Humans', 'Kinetics', 'Male', 'Sports']
| 8,809,716
|
[['M01.060.116'], ['E05.318.740.150', 'N05.715.360.750.125', 'N06.850.520.830.150'], ['I03.450.642.845.117'], ['G01.154.090', 'G01.374.089'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G01.374.661', 'G02.111.490'], ['I03.450.642.845']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 0
|
Socioeconomic inequalities in mental well-being among Hungarian adolescents: a cross-sectional study.
|
INTRODUCTION: According to several empirical studies, mental well-being is significant in adolescence; adolescent's social network is undergoing radical changes while at the same time depression is increasing. The primary goal of our study is to determine whether socioeconomic status (SES) is associated with mental health status of Hungarian adolescents and the strength and nature of this association.METHODS: Our sample was comprised of three high schools of Debrecen (the second largest city of Hungary). Data were collected in January 2013. In all, 471 students filled out the questionnaire from 22 classes (14-18 years old). 'Absolute' (education and occupational status of the parents, assessed by the adolescent) and 'subjective' (self-assessment of family's social class) SES measures and five mental health indicators (shyness, loneliness, need to belong, psychosomatic symptoms, self-esteem) were involved. Descriptive statistics and binary logistic regression analyses were used to examine the relationships between family SES and mental health indicators.RESULTS: Our results indicate that association between adolescents' 'subjective' SES and mental well-being is not gradient-like. Manual employment and unemployment status of both parents also proved to be significant determinants of mental health status.CONCLUSIONS: According to our results, professionals of school-based mental health programs should consider students whose parents are unemployed or have manual occupational status as a high risk group in terms of mental well-being.
|
['Adolescent', 'Female', 'Health Status Disparities', 'Humans', 'Hungary', 'Male', 'Mental Health', 'Psychology, Adolescent', 'Social Class', 'Social Determinants of Health', 'Surveys and Questionnaires']
| 25,348,821
|
[['M01.060.057'], ['I01.240.425.675', 'N01.224.425.437', 'N06.850.505.400.425.675'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['Z01.542.248.495'], ['F02.418', 'N01.400.500'], ['F04.096.628.065'], ['I01.880.853.996.755', 'N01.824.782'], ['N01.224.425.762', 'N01.400.675'], ['E05.318.308.980', 'N05.715.360.300.800', 'N06.850.520.308.980']]
|
['Named Groups [M]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]', 'Organisms [B]', 'Geographicals [Z]', 'Psychiatry and Psychology [F]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 1
|
[Basal and stress-induced adrenocortical activity in young pigeons during pre- and post-hatching periods (author's transl)].
|
The functioning of the hypothalamic pituitary-adrenocortical system was tested in young pigeons before and after hatching, by radiocompetitive assay of plasma corticosterone (B) at rest and under stress. 1 degree B was expressed in ng/ml. Resting adult values (8-10) could not be observed before the birds were 6 week-old. B levels were very low 36 h before and 24 and 48 h after hatching (3-5). Then, they progressively increased. 2 degrees B concentrations soared during the 24 h immediately preceding hatching to rise to 45 ng/ml at the time of hatching. After that, corticosteronemia rapidly decreased. 3 degrees Neurogenic stress was applied by means of electrical stimulation of the led skin for 15 sec. Ten to 12 min later, B rose markedly (30 ng/ml) in 6 week-old pigeons as well as in adult ones. No response could be observed from 36 h before to 24 h after hatching. Significant adrenocortical activation was obtained in 3-7 day old squabs (18 ng/ml). 4 degrees Systemic stress (ether vapor) led to significant hypercorticosteronemia (30-40 ng) in pre-hatching embryos. No response could be observed for 24 h after hatching. Ether-induced adrenocortical activation appeared again in 2-3 day-old pigeons. 5 degrees The development of both resting and stress-altered corticosteronemia did not seem to be related to the increase in body weight of young birds. One can present the hypothesis that a strongly stressfull situation occurring during the period which immediately precedes hatching, results in a rapid activation of the adrenocortex. The period following hatching corresponds to the "non stress responsive period" of mammals and could be explained by some feedback mechanisms.
|
['Animals', 'Columbidae', 'Corticosterone', 'Female', 'Male', 'Pituitary-Adrenal System', 'Stress, Physiological']
| 7,411,484
|
[['B01.050'], ['B01.050.150.900.248.165.150'], ['D04.210.500.745.745.654.237', 'D06.472.040.585.353.237'], ['A06.300.691'], ['G07.775']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Exercise training and myocardial remodeling in patients with reduced ventricular function: one-year follow-up with magnetic resonance imaging.
|
BACKGROUND: Exercise training is now an accepted therapeutic intervention in patients with reduced ventricular function after a myocardial infarction. However, there are conflicting reports on the effects of training on the remodeling process of the heart, and previous studies have only assessed short-term effects of training.METHODS AND RESULTS: Twenty-five patients with reduced ventricular function after myocardial infarction were randomly assigned to an intensive 2-month exercise training program or to a control group (control group: n = 13, aged 55 +/- 7 years, ejection fraction 33.3% +/- 6%; exercise group: n = 12, aged 56 +/- 5 years, ejection fraction 31.5% +/- 7%) and followed up for 1 year. Measures of left ventricular size, function, and wall thickness in the infarct and noninfarct areas were made by magnetic resonance imaging at baseline, after the 2-month training period, and 1 year later. Maximal oxygen uptake increased in the trained group, from 19.7 +/- 3 mL/kg per minute at baseline to 25.1 +/- 5 and 24.2 +/- 5 mL/kg per minute after 2 months and 1 year, respectively (P <.05 vs baseline for both), whereas the control group did not change significantly. Ejection fraction, end-diastolic volumes, and end-systolic volumes did not change at any measurement point throughout the study period in either the trained or control groups. Myocardial wall thickness measurements at end-diastole and end-systole and their differences determined by magnetic resonance imaging yielded no significant interactions between groups. When myocardial wall thickness measurements were classified by infarct or noninfarct areas, no differences were observed between groups over the study period.CONCLUSIONS: Intensive exercise training in patients with reduced ventricular function resulted in a significant improvement in exercise capacity after 2 months, and this improvement was sustained over 1 year. In contrast to some recent reports, training had no deleterious effects on left ventricular volume, function, or wall thickness regardless of infarct area.
|
['Exercise Test', 'Exercise Therapy', 'Follow-Up Studies', 'Hemodynamics', 'Humans', 'Magnetic Resonance Imaging, Cine', 'Male', 'Middle Aged', 'Myocardial Infarction', 'Myocardium', 'Randomized Controlled Trials as Topic', 'Treatment Outcome']
| 10,650,298
|
[['E01.370.370.380.250', 'E01.370.386.700.250', 'E05.333.250'], ['E02.760.169.063.500.387', 'E02.779.483', 'E02.831.535.483'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['G09.330.380'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.350.825.500.510'], ['M01.060.116.630'], ['C14.280.647.500', 'C14.907.585.500', 'C23.550.513.355.750', 'C23.550.717.489.750'], ['A02.633.580', 'A07.541.704', 'A10.690.552.750'], ['E05.318.372.250.250.365.500', 'N05.715.360.330.250.250.365.500', 'N06.850.520.450.250.250.365.500'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Named Groups [M]', 'Diseases [C]', 'Anatomy [A]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Evidence for a deployment-related Gulf War syndrome by factor analysis.
|
To identify a syndrome unique to Gulf War veterans, the authors applied an exploratory factor analysis to the 47-symptom correlation matrix of 10,423 Gulf War and 8,960 non-Gulf War veteran respondents. A separate factor analysis was performed for Gulf War and non-Gulf War veterans, and the resulting 6 factors were compared between the 2 groups. Five of the factors were very similar in the 2 groups; however, 1 of the factors in the Gulf War group, but not the non-Gulf War group, contained a cluster of symptoms consistent with neurological impairment. Symptoms specific to this factor were blurred vision, loss of balance/dizziness, tremors/shaking, and speech difficulty. The Gulf War veterans who had all of the aforementioned symptoms (n = 277) also reported exposures to several putative risk factors at a rate 3 or more times higher than other Gulf War veterans. This finding suggests a possible syndrome related to Gulf War deployment, which requires objective supporting clinical evidence.
|
['Adult', 'Chi-Square Distribution', 'Factor Analysis, Statistical', 'Female', 'Health Surveys', 'Humans', 'Male', 'Military Medicine', 'Nervous System Diseases', 'Persian Gulf Syndrome', 'Surveys and Questionnaires', 'Veterans']
| 12,071,362
|
[['M01.060.116'], ['E05.318.740.994.300', 'G17.820.300', 'N05.715.360.750.750.200', 'N06.850.520.830.994.300'], ['E05.318.740.400', 'N05.715.360.750.350', 'N06.850.520.830.400'], ['E05.318.308.980.438', 'N05.715.360.300.800.438', 'N06.850.520.308.980.438'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['H02.403.500'], ['C10'], ['C24.653'], ['E05.318.308.980', 'N05.715.360.300.800', 'N06.850.520.308.980'], ['M01.930']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Organisms [B]', 'Disciplines and Occupations [H]', 'Diseases [C]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 1
| 1
| 0
|
Optimized preparation of vinpocetine proliposomes by a novel method and in vivo evaluation of its pharmacokinetics in New Zealand rabbits.
|
Free-flowing proliposomes which contained vinpocetine were prepared successfully to increase the oral bioavailability of vinpocetine. In this study the proliposomes were prepared by a novel method which was reported for the first time and the formulation was optimized using the centre composite design (CCD). The optimized formulation was Soybean phosphatidylcholine: 860 mg; cholesterol: 95 mg and sorbitol: 8000 mg. After the proliposomes were contacted with water, the suspension of vinpocetine liposomes formed automatically and the entrapment efficiency was approximately 86.3% with an average particle size of about 300 nm. The physicochemical properties of the proliposomes including SEM, TEM, XRD and FTIR were also detected. HPLC system was applied to study the concentration of vinpocetine in the plasma of the New Zealand rabbits after oral administration of vinpocetine proliposomes and vinpocetine suspension. The pharmacokinetic parameters were calculated by the software program DAS2.0. The concentration-time curves of vinpocetine suspension and vinpocetine proliposomes were much more different. There were two absorption peaks on the concentration-time curves of the vinpocetine proliposomes. The pharmacokinetic parameters of vinpocetine and vinpocetine proliposomes in New Zealand rabbits were T(max) 1 h and 3 h (there was also an absorption peak at 1 h); C(max) 163.82+/-12.28 ng/ml and 166.43+/-21.04 ng/ml; AUC(0-infinity) 1479.70+/-68.51 ng/ml h and 420.70+/-35.86 ng/ml h, respectively. The bioavailability of vinpocetine in proliposomes was more than 3.5 times higher than the vinpocetine suspension. The optimized vinpocetine proliposomes did improve the oral bioavailability of vinpocetine in New Zealand rabbits and offer a new approach to enhance the gastrointestinal absorption of poorly water soluble drugs.
|
['Administration, Oral', 'Animals', 'Area Under Curve', 'Biological Availability', 'Drug Carriers', 'Drug Delivery Systems', 'Drug Evaluation, Preclinical', 'Drug Stability', 'Liposomes', 'Male', 'Nootropic Agents', 'Particle Size', 'Rabbits', 'Technology, Pharmaceutical', 'Temperature', 'Time Factors', 'Vinca Alkaloids']
| 19,651,165
|
[['E02.319.267.100'], ['B01.050'], ['E05.318.740.200', 'G03.787.101', 'G07.690.725.064', 'N06.850.520.830.200'], ['G03.787.151', 'G07.690.725.129'], ['D26.255.260', 'E02.319.300.380'], ['E02.319.300'], ['E05.290.750', 'E05.337.550'], ['E05.916.330'], ['D25.479.517', 'D26.255.260.517', 'J01.637.051.479.517', 'J01.637.087.500.517'], ['D27.505.954.427.637'], ['G02.712'], ['B01.050.150.900.649.313.968.700'], ['E05.916', 'J01.897.836'], ['G01.906.595', 'G16.500.275.063.725.710', 'G16.500.750.775.710', 'N06.230.150.450', 'N06.230.300.100.725.710'], ['G01.910.857'], ['D03.132.436.681.827', 'D03.633.100.473.402.681.827', 'D03.633.100.496.500.500.681.827']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Chemicals and Drugs [D]', 'Technology, Industry, and Agriculture [J]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
|
Patient-level Predictors of Extent of Exposure to a Community Health Worker Intervention in a Randomized Controlled Trial.
|
Objective: Community health worker (CHW) interventions have been cited as a best practice for reducing health disparities, but patient-level attributes may contribute to differential uptake. We examined patient characteristics associated with the extent of exposure to a CHW coaching intervention among a predominantly low-income, African American population participating in a randomized controlled trial of hypertension interventions.Design: We conducted a within-group longitudinal analysis of those receiving a CHW intervention from a study conducted between September 2003 and August 2005. We employed mixed effects models to ascertain relationships between patients' characteristics, length of time spent with the CHW, and the number of topics discussed during the intervention.Setting: Baltimore, MD.Participants: 140 patients with a diagnosis of hypertension in the CHW intervention arm.Results: Marital status, stress, depression symptomology, and having multiple comorbid conditions were each independently and positively related to the length of time patients spent with CHWs. An indirect relationship between higher perceived physical health and time spent with the CHW was observed. Patients with multiple comorbid conditions discussed more intervention-related topics, while patients who perceived themselves as being healthier discussed fewer topics. Marital status and extreme poverty were the strongest predictors of the length of time spent with the CHW, while having multiple comorbid conditions was the strongest predictor of the number of coaching topics discussed.Conclusions: Differential exposure to a CHW intervention is influenced by patients' physical, psychosocial, and sociodemographic characteristics.
|
['Adult', 'African Americans', 'Baltimore', 'Community Health Workers', 'Female', 'Health Education', 'Humans', 'Hypertension', 'Male', 'Middle Aged', 'Minority Health', 'Poverty']
| 31,057,311
|
[['M01.060.116'], ['M01.686.508.100.100', 'M01.686.754.100'], ['Z01.107.567.875.500.500.100', 'Z01.433.100'], ['M01.526.485.067.080', 'N02.360.067.080'], ['I02.233.332', 'N02.421.726.407'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C14.907.489'], ['M01.060.116.630'], ['N01.400.512'], ['I01.880.735.634', 'I01.880.853.996.535', 'N01.824.600']]
|
['Named Groups [M]', 'Geographicals [Z]', 'Health Care [N]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 1
|
Metastatic involvement of parotid from carcinoma of the breast--a case report.
|
INTRODUCTION: Involvement of parotid gland by metastases from distant primary malignant tumors is rare.CASE REPORT: A 42-year-old female having right breast cancer had reported to the Department of Oral Diagnosis and Medicine with a chief complaint of a swelling in the left parotid gland region for 2 months. On the basis of clinical findings, the primary malignancy of parotid gland was provisionally suspected.DISCUSSION: Microscopic examination of parotid and breast revealed that parotid gland was involved as a distant metastases from a breast carcinoma, indicating that a swelling in the parotid needs to be assessed and investigated very carefully.
|
['Adult', 'Biopsy', 'Breast', 'Breast Neoplasms', 'Carcinoma, Ductal, Breast', 'Diagnosis, Differential', 'Female', 'Humans', 'India', 'Neck Dissection', 'Parotid Gland', 'Parotid Neoplasms']
| 19,190,940
|
[['M01.060.116'], ['E01.370.225.500.384.100', 'E01.370.225.998.054', 'E01.370.388.100', 'E04.074', 'E05.200.500.384.100', 'E05.200.998.054', 'E05.242.384.100'], ['A01.236'], ['C04.588.180', 'C17.800.090.500'], ['C04.557.470.200.025.232.500', 'C04.557.470.615.132.500', 'C04.588.180.390', 'C17.800.090.500.390'], ['E01.171'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['Z01.252.245.393'], ['E04.446.318', 'E04.580.411'], ['A03.556.500.760.464', 'A10.336.779.464', 'A14.549.760.464'], ['C04.588.443.591.824.695', 'C07.465.530.824.695', 'C07.465.815.470.770', 'C07.465.815.718.589']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Diseases [C]', 'Organisms [B]', 'Geographicals [Z]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 1
|
Lactoferrin works as a new LPS-binding protein in inflammatory activation of macrophages.
|
Though lactoferrin (LF) is a glycoprotein that is involved in immunomodulation, its action mechanism has not been fully elucidated. Previous studies have suggested that lipopolysaccharide (LPS) activity is inhibited by direct binding between LPS and LF. However, here we show that when LPS and purified LF was mixed, and formed a complex (termed as LF-LPS), it was found to induce production of inflammatory mediators in macrophages to some extent, rather than inhibit LPS activity. Moreover, when macrophages were pretreated with LF-LPS, cells were rendered a tolerant state to LPS challenge. These macrophage-activating effects were mediated by Toll-like receptor 4 (TLR4)-NF-kappaB pathway. Comparative studies with C3H/HeN and C3H/HeJ mice demonstrated the strong dependency of the LF-LPS signal on TLR4. These findings suggest that the immunomodulatory properties of LF could be due, in part, to LPS binding.
|
['Acute-Phase Proteins', 'Animals', 'Blotting, Western', 'Carrier Proteins', 'Cells, Cultured', 'Electrophoretic Mobility Shift Assay', 'Immunoassay', 'Inflammation', 'Lactoferrin', 'Lipopolysaccharides', 'Macrophage Activation', 'Membrane Glycoproteins', 'Mice', 'Mice, Inbred C3H', 'NF-kappa B', 'Nitrites', 'Receptors, Cell Surface', 'Reverse Transcriptase Polymerase Chain Reaction', 'Signal Transduction', 'Toll-Like Receptor 4', 'Toll-Like Receptors', 'Tumor Necrosis Factor-alpha']
| 15,251,114
|
[['D12.776.124.050'], ['B01.050'], ['E05.196.401.143', 'E05.301.300.096', 'E05.478.566.320.200', 'E05.601.262', 'E05.601.470.320.200'], ['D12.776.157'], ['A11.251'], ['E05.196.401.500'], ['E05.478.566', 'E05.601.470'], ['C23.550.470'], ['D08.811.277.656.300.760.471', 'D08.811.277.656.959.350.471', 'D12.776.157.427.750.249', 'D12.776.256.159.750.816.500.507', 'D12.776.377.457.507', 'D12.776.395.507', 'D12.776.556.579.750.249'], ['D09.400.500', 'D09.698.718.450', 'D10.494', 'D23.050.161.616.525', 'D23.946.123.329.500'], ['G12.287.500'], ['D12.776.395.550', 'D12.776.543.550'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.388', 'B01.050.150.900.649.313.992.635.505.500.400.388'], ['D05.500.672', 'D12.776.260.600', 'D12.776.660.600', 'D12.776.930.600'], ['D01.248.497.158.635', 'D01.625.600.600', 'D02.633'], ['D12.776.543.750'], ['E05.393.620.500.725'], ['G02.111.820', 'G04.835'], ['D12.776.543.750.705.910.500.400'], ['D12.776.543.750.705.910.500'], ['D12.644.276.374.500.800', 'D12.644.276.374.750.626', 'D12.776.124.900', 'D12.776.395.930', 'D12.776.467.374.500.800', 'D12.776.467.374.750.626', 'D23.529.374.500.800', 'D23.529.374.750.626']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Diseases [C]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Choosing the best needle for diagnostic lumbar puncture.
|
A new generation of Atraumatic (blunt tipped) needles now exists in addition to the traditional Quincke (cutting tip) needles for LP. We wished to identify the optimal size and type of needle that should be used for this technique. Requirements include rapid and accurate transduction of CSF pressure and adequate flow rate for CSF collection purposes while minimizing headache and other neurologic sequelae. Different types of available Atraumatic and Quincke needles were tested in two models that simulated normal and raised CSF pressures. The flow rates and the time required for an accurate transduction of CSF pressure onto a standard spinal manometer through each needle type was measured. Overall, Atraumatic-tipped needles compared favorably with similarly sized Quincke needles tested. The 20-gauge needles displayed suitable flow and pressure transduction characteristics. Some of the 22-gauge Atraumatic needles rapidly measured CSF pressure, but their flow rates were only suitable for small volume CSF collection. There is extensive literature to support that neurologic sequelae are reduced using Atraumatic needles. Diagnostic LP can be easily and accurately performed by using a large Atraumatic needle with the potential for considerable reduction in post-LP headache and related neurologic sequelae.
|
['Humans', 'Needles', 'Spinal Puncture']
| 8,710,120
|
[['B01.050.150.900.649.313.988.400.112.400.400'], ['E07.612'], ['E01.370.225.998.054.790', 'E01.370.376.700', 'E02.800.779', 'E04.074.790', 'E04.665.700', 'E05.200.998.054.790']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Feature-specific terrain park-injury rates and risk factors in snowboarders: a case-control study.
|
BACKGROUND: Snowboarding is a popular albeit risky sport and terrain park (TP) injuries are more severe than regular slope injuries. TPs contain man-made features that facilitate aerial manoeuvres. The objectives of this study were to determine overall and feature-specific injury rates and the potential risk factors for TP injuries.METHODS: Case-control study with exposure estimation, conducted in an Alberta TP during two ski seasons. Cases were snowboarders injured in the TP who presented to ski patrol and/or local emergency departments. Controls were uninjured snowboarders in the same TP. ê Statistics were used to measure the reliability of reported risk factor information. Injury rates were calculated and adjusted logistic regression was used to calculate the feature-specific odds of injury.RESULTS: Overall, 333 cases and 1261 controls were enrolled. Reliability of risk factor information was ê>0.60 for 21/24 variables. The overall injury rate was 0.75/1000 runs. Rates were highest for jumps and half-pipe (both 2.56/1000 runs) and lowest for rails (0.43/1000 runs) and quarter-pipes (0.24/1000 runs). Compared with rails, there were increased odds of injury for half-pipe (OR 9.63; 95% CI 4.80 to 19.32), jumps (OR 4.29; 95% CI 2.72 to 6.76), mushroom (OR 2.30; 95% CI 1.20 to 4.41) and kickers (OR 1.99; 95% CI 1.27 to 3.12).CONCLUSIONS: Higher feature-specific injury rates and increased odds of injury were associated with features that promote aerial manoeuvres or a large drop to the ground. Further research is required to determine ways to increase snowboarder safety in the TP.
|
['Adolescent', 'Adult', 'Alberta', 'Case-Control Studies', 'Child', 'Female', 'Humans', 'Incidence', 'Injury Severity Score', 'Male', 'Risk Factors', 'Skiing']
| 24,184,587
|
[['M01.060.057'], ['M01.060.116'], ['Z01.107.567.176.064'], ['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['M01.060.406'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.308.985.525.375', 'N01.224.935.597.500', 'N06.850.505.400.975.525.375', 'N06.850.520.308.985.525.375'], ['E05.318.308.940.968.875.500', 'E05.944.600', 'N04.452.859.564.800.500', 'N05.715.360.300.715.500.800.400'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['I03.450.642.845.787.500']]
|
['Named Groups [M]', 'Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Anthropology, Education, Sociology, and Social Phenomena [I]']
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 1
|
Phase II study of denileukin diftitox for relapsed/refractory B-Cell non-Hodgkin's lymphoma.
|
PURPOSE: Denileukin diftitox is a fusion protein combining diphtheria toxin and interleukin-2 (IL-2) that targets tumor cells expressing the IL-2 receptor. Its efficacy has been shown in CD25+ cutaneous T-cell lymphoma, but not in B-cell non-Hodgkin's lymphoma (NHL). A phase II study was performed to evaluate the efficacy and tolerability of denileukin diftitox for relapsed or refractory B-cell NHL.PATIENTS AND METHODS: Patients with relapsed or refractory B-cell NHL were eligible. Tumor CD25 expression was determined by immunohistochemistry or flow cytometry. Denileukin diftitox was administered intravenously at a dose of 18 microg/kg once daily for 5 days every 3 weeks, up to eight cycles.RESULTS: Of the 45 patients assessable for response, 32 (71%) were refractory to the last chemotherapy treatment, and all were previously treated with rituximab. Three complete responses (6.7%) and eight partial responses (17.8%) were observed, for an overall response rate of 24.5%. Nine patients (20%) had stable disease. Objective response rates were similar in CD25+ (22%) and CD25- histologies (29%), as were stable disease rates (22% and 18%, respectively). For responding patients, the median time to treatment failure was 7 months, with a median follow-up in survivors of 18 months (range, 9 to 28 months), and the projected progression-free survival at 20 months was 24% (95% CI, 0% to 60%). Most toxicities were low-grade and transient.CONCLUSION: Denileukin diftitox seems to be effective in relapsed or refractory, CD25+ and CD25- B-cell NHL and is well-tolerated at the dosage evaluated. Evaluation of denileukin diftitox in combination with other agents may be warranted.
|
['Adult', 'Aged', 'Antineoplastic Agents', 'CD4 Antigens', 'CD8 Antigens', 'Diphtheria Toxin', 'Disease-Free Survival', 'Drug Administration Schedule', 'Female', 'Humans', 'Hypoalbuminemia', 'Interleukin-2', 'Lymphoma, B-Cell', 'Male', 'Middle Aged', 'Neoplasm Recurrence, Local', 'Receptors, Interleukin-2', 'Recombinant Fusion Proteins', 'Treatment Outcome']
| 15,353,540
|
[['M01.060.116'], ['M01.060.116.100'], ['D27.505.954.248'], ['D12.776.543.750.705.852.420.810.500', 'D12.776.543.750.830.700.025', 'D23.050.301.264.894.100', 'D23.101.100.894.100'], ['D23.050.301.264.894.108', 'D23.101.100.894.108'], ['D08.811.913.400.725.115.220', 'D23.946.123.305'], ['E01.789.800.190', 'E05.318.740.998.300', 'N04.761.559.590.800.190', 'N05.715.360.575.575.800.190', 'N05.715.360.750.795.300', 'N06.850.520.830.998.300'], ['E02.319.283'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C15.378.147.607.500'], ['D12.644.276.374.465.021', 'D12.644.276.374.480.372', 'D12.776.467.374.465.021', 'D12.776.467.374.480.372', 'D23.529.374.465.155', 'D23.529.374.480.372'], ['C04.557.386.480.150', 'C15.604.515.569.480.150', 'C20.683.515.761.480.150'], ['M01.060.116.630'], ['C04.697.655', 'C23.550.727.655'], ['D12.776.543.750.705.852.420.320'], ['D12.776.828.300'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Cloning and sequencing of the genes encoding the light-harvesting B806-866 polypeptides and initial studies on the transcriptional organization of puf2B, puf2A and puf2C in Chloroflexus aurantiacus.
|
The genes encoding the alpha- and beta-polypeptide subunits of the B806-866 membrane-bound light-harvesting complex of Chloroflexus aurantiacus have been cloned and the nucleotide sequences determined. The gene puf2A, which encodes the B806-866 alpha-polypeptide, began 28 bases downstream of the stop codon of puf2B, which encodes the B806-866 beta gene. The gene-encoding cytochrome c-554, puf2C, was found about 250 bp downstream of puf2A. puf2A encoded a 13 amino acid extension at the C-terminus of the B806-866 alpha-polypeptide that was not present in the mature protein. These genes, unlike those of purple nonsulfur bacteria, did not form a contiguous operon with puf1L or puf1M, the genes encoding the L and M subunits of the photochemical reaction center. The occurrence of the two latter genes and of puf2B and puf2A in two separate operons has not been observed in purple bacteria. Under photoheterotrophic growth conditions, puf2B and puf2A were encoded on an abundant mRNA that was 0.5 kb long. Two monocistronic transcripts for puf2C were observed that had different 5'-ends. One transcript encoding all three genes was also detected. Nucleotide sequences very similar to the consensus promoter sequence of the Escherichia coli RNA polymerase sigma 70 subunit were found seven and eight bases upstream of the 5'-end of mRNA encoding puf2B and for one of the monocistronic mRNA encoding puf2C, respectively.
|
['Amino Acid Sequence', 'Bacteria', 'Bacterial Proteins', 'Base Sequence', 'Cloning, Molecular', 'Cytochrome c Group', 'Genes, Bacterial', 'Molecular Sequence Data', 'Photosynthetic Reaction Center Complex Proteins', 'Pigments, Biological', 'RNA, Bacterial', 'RNA, Messenger', 'Sequence Analysis, DNA', 'Sequence Homology, Nucleic Acid', 'Transcription, Genetic']
| 7,535,995
|
[['G02.111.570.060', 'L01.453.245.667.060'], ['B03'], ['D12.776.097'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['E05.393.220'], ['D08.244.286', 'D12.776.422.220.286'], ['G05.360.340.024.340.364.249', 'G05.360.340.358.024.249', 'G05.360.340.358.207.249'], ['L01.453.245.667'], ['D05.500.562.488', 'D08.811.600.710', 'D12.776.543.930.500', 'D12.776.765.199.750.750'], ['D23.767'], ['D13.444.735.473'], ['D13.444.735.544'], ['E05.393.760.700'], ['G02.111.810.550', 'G05.810.550'], ['G02.111.873', 'G05.297.700']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Determination of the secondary structure of Kluyveromyces lactis beta-galactosidase by circular dichroism and its structure-activity relationship as a function of the pH.
|
The secondary structure of Kluyveromyces lactis beta-galactosidase was determined by circular dichroism. It is mainly a beta-type protein, having 22% beta-turns, 14% parallel beta-sheet, 25% antiparallel beta-sheet, 34% unordered structure, and only 5% alpha-helix. The structure-activity relationship as a function of the pH was also studied. The pH conditions leading to the highest secondary structure content (100% ellipticity) of the enzyme was found at pH 7.0; at pH 6.5-7.0, the percent ellipticity decreased slightly, suggesting little structural change, but the activity decreased significantly, probably because of variations in critical residues. On the other hand, at pH's above 7.0, a more noticeable change in ellipticity was observed due to structural changes; the CD analysis showed a small increase in the helical content toward higher pH, whereas the maximum activity was found at pH 7.5, meaning that the changes produced in the secondary structure at this pH favored the interaction between the enzyme and the substrate.
|
['Chromatography, Gel', 'Circular Dichroism', 'Electrophoresis, Polyacrylamide Gel', 'Fungal Proteins', 'Hydrogen-Ion Concentration', 'Kluyveromyces', 'Lactase', 'Protein Conformation', 'Structure-Activity Relationship', 'beta-Galactosidase']
| 16,366,715
|
[['E05.196.181.400.250'], ['E05.196.867.151'], ['E05.196.401.402', 'E05.301.300.319'], ['D12.776.354'], ['G02.300'], ['B01.300.107.795.536', 'B01.300.930.536'], ['D08.811.277.450.410.100.500'], ['G02.111.570.820.709'], ['G02.111.830', 'G07.690.773.997'], ['D08.811.277.450.410.100']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Glucocorticoid-induced alternative promoter usage for a novel 5' variant of granzyme A.
|
Glucocorticoids exert diverse physiological functions through transcriptional regulation of genes including granzyme A (GZMA). GZMA is one of the apoptotic effectors localized in cytotoxic T lymphocytes and is considered to mediate glucocorticoid-induced apoptosis of human leukemia 697 cells. In the present study, we identified a novel 5' variant transcript of GZMA in dexamethasone (DEX)-treated 697 cells. We designated this novel transcript as GZMAbeta. The transcription of GZMAbeta starts at 290 bp downstream of the first intronic glucocorticoid response element (GRE). Chromatin immunoprecipitation assay showed that glucocorticoid receptor (GR) binds to the intronic GRE in a DEX-dependent manner. Luciferase assay and RT-PCR also showed that DEX induces GZMAbeta transcription mediated by GR binding to the intronic GRE. Our results show that there exist at least two transcripts in human GZMA, whose expression is differentially regulated by glucocorticoid.
|
["5' Untranslated Regions", 'Alternative Splicing', 'Base Sequence', 'Chromatin Immunoprecipitation', 'Dexamethasone', 'Gene Expression Regulation', 'Genetic Variation', 'Granzymes', 'Humans', 'Introns', 'Luciferases', 'Molecular Sequence Data', 'Promoter Regions, Genetic', 'Receptors, Glucocorticoid', 'Regulatory Sequences, Nucleic Acid', 'Response Elements', 'Transcription, Genetic', 'Transcriptional Activation']
| 17,180,578
|
[['D13.444.735.544.875.885', 'D13.444.735.790.878.885', 'G05.360.340.024.220.880.885', 'G05.360.340.024.340.137.910.885'], ['G02.111.760.700.100', 'G03.839.700.100', 'G05.308.700.700.100'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['E05.393.170', 'E05.478.605.160'], ['D04.210.500.745.432.769.344', 'D04.210.500.908.238'], ['G05.308'], ['G05.365'], ['D08.811.277.656.300.760.397', 'D08.811.277.656.959.350.397'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G05.360.340.024.220.400', 'G05.360.340.024.340.137.515'], ['D08.811.682.517', 'D12.776.532.510'], ['L01.453.245.667'], ['G02.111.570.080.689.675', 'G05.360.080.689.675', 'G05.360.340.024.340.137.750.680'], ['D12.776.826.750.430'], ['G02.111.570.080.689', 'G05.360.080.689'], ['G02.111.570.080.689.330.700', 'G02.111.570.080.689.675.700', 'G05.360.080.689.330.700', 'G05.360.080.689.675.700', 'G05.360.340.024.340.137.750.249.765', 'G05.360.340.024.340.137.750.680.765'], ['G02.111.873', 'G05.297.700'], ['G05.308.800']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Intravenous feeding in a gastroenterological unit: a prospective study of infective complications.
|
We have assessed the bacteriological safety of a system of intravenous feeding by culturing catheters on removal, swabs taken from the catheter's skin entry sites, and samples of infusion fluid. Among 38 treatment periods using 51 catheters over 1551 patient days, septicaemia due to Staphylococcus aureus was observed in one treatment period and bacteraemias due to Staphylococcus albus and Diphtheroid species in two others. The Staph. aureus and the diphtheroids probably gained access via the skin entry site along the outside of the catheter. The origin of the Staph. albus was uncertain. Parenteral nutrition over extended periods can be a safe procedure if aseptic precautions are taken. The importance of the catheter's skin entry site as a source of contaiminating organisms is emphasised.
|
['Adult', 'Asepsis', 'Bacteria', 'Bacterial Infections', 'Catheterization', 'Female', 'Humans', 'Male', 'Middle Aged', 'Parenteral Nutrition', 'Prospective Studies', 'Sepsis', 'Skin', 'Staphylococcal Infections']
| 112,124
|
[]
|
[]
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[Factors affecting exercise capacity after coronary bypass grafting].
|
In order to determine the contribution of cardiac reserve and the peripheral muscle to exercise capacity in patients after Coronary Artery Bypass Grafting (CABG), 19 patients (18 males, 1 female, mean age 63.3 +/- 7.1 years, mean numbers of grafting 2.5 +/- 0.8) performed exercise tests at 1 week, 3 weeks, and 3 months after CABG. Ventilatory gas was analyzed throughout the testing and anaerobic threshold (AT) and peak oxygen uptake (peak VO2) was determined. During exercise testing, the cardiac index (CI) was measured, and the change in CI during exercise, delta CI = (CI at peak exercise) (CI at rest), was calculated. O2 delivery was derived from the product of CO and the oxygen content of arterial blood at peak exercise. The sectional area of the thigh muscles at the level of 10 cm above the patella was measured using a computed tomography before each test. Average peak VO2 at 1 week after CABG was 867 +/- 171 ml/min and it increased to 1,214 +/- 246 ml/min at 6 months. Average AT did not change from 1 week to 3 weeks, however, it increased significantly from 665 +/- 122 ml/min at 3 weeks to 873 +/- 181 ml/min at 6 months. The muscle area of the thigh increased significantly from 170 +/- 24 cm2 at 3 weeks after CABG to 186 +/- 27 cm2 at 3 months. delta CI showed a tendency to increase from 6.6 +/- 1.2 l/min/m2 at 1 week after CABG to 7.3 +/- 1.3 l/min/m2 at 3 weeks, and also showed a tendency to increase from 3 weeks to 6 months. Peak VO2 and AT correlated to delta CI at 1 weeks and also it correlated significantly to both the muscle area of the thigh and delta CI at 3 weeks, 3 months, and 6 months after CABG. The delta value of peak VO2 from 1 week to 3 weeks showed a significant correlation to those of delta CI and O2 delivery. Moreover, the delta values of peak VO2 and AT from 3 weeks to 3 months showed a correlation to those of delta CI and O2 delivery. These results suggest that both cardiac reserve and peripheral factors contribute to the exercise capacity up to 3 months after CABG, and, in particular, O2 delivery are important to increase exercise capacity.
|
['Cardiac Output', 'Coronary Artery Bypass', 'Exercise Test', 'Exercise Tolerance', 'Female', 'Hemodynamics', 'Humans', 'Male', 'Middle Aged', 'Muscle, Skeletal', 'Myocardial Ischemia', 'Oxygen Consumption', 'Postoperative Period']
| 9,185,439
|
[['E01.370.370.380.150', 'G09.330.380.124'], ['E04.100.376.719.332', 'E04.100.814.868.750', 'E04.928.220.520.220'], ['E01.370.370.380.250', 'E01.370.386.700.250', 'E05.333.250'], ['G11.427.680.270'], ['G09.330.380'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['A02.633.567', 'A10.690.552.500'], ['C14.280.647', 'C14.907.585'], ['G03.680'], ['E04.614.750', 'N02.421.585.753.750']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Named Groups [M]', 'Anatomy [A]', 'Diseases [C]', 'Health Care [N]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
[Volatile oil of Anethum Graveolens L. as an inhibitor of yeast and lactic acid bacteria].
|
The antimicrobial activity of 25 volatile oils from aerial parts and seeds of dill (Anethum graveolens L.) of different geographical origin towards yeast Saccharomyces vini and lactic acid bacteria Lactobacterium buchneri was measured by serial dilutions. Volatile oils from mature seeds and green parts of the plants harvested at late vegetation phases showed the highest activity. The geographical origin of plants influenced insignificantly the antimicrobial activity of volatile oil.
|
['Cell Division', 'Lactobacillus', 'Oils, Volatile', 'Plants', 'Saccharomyces', 'Seeds', 'Species Specificity']
| 1,208,405
|
[['G04.144.220', 'G04.161.750.500', 'G05.113', 'G07.345.249.410.750.500'], ['B03.353.750.450.475', 'B03.510.460.400.410.475.475', 'B03.510.550.450.475'], ['D10.627.675'], ['B01.650'], ['B01.300.107.795.785', 'B01.300.930.705'], ['A18.024.500.750', 'G07.203.300.775', 'J02.500.775'], ['G16.824']]
|
['Phenomena and Processes [G]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Technology, Industry, and Agriculture [J]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
[Role and mechanism of mitophagy in ventilator-induced lung injury in rats].
|
OBJECTIVE: To explore the role and mechanism of mitophagy in ventilator-induced lung injury (VILI) in rats.METHODS: Thirty-six adult Sprague-Dawley (SD) rats were randomly divided into three groups (each n = 12): spontaneous breathing group (CON group), normal tidal volume (VT) group (NVT group, VT was 8 mL/kg) and high VT group (HVT group, VT was 40 mL/kg). All rats received endotracheal tube by tracheostomy. The rats in CON group were maintained spontaneous breathing, while those in NVT and HVT groups received mechanical ventilation with corresponding VT. After 4 hours of ventilation, the serum, bronchoalveolar lavage fluid (BALF) and lung tissues were harvested. The lung wet/dry weight (W/D) ratio was assessed, and the histopathology changes were observed by light microscopy, and the ultra structure changes in type II alveolar epithelial cell (AEC II) were observed by electron microscopy. The levels of interleukin (IL-1â and IL-6) and tumor necrosis factor-á (TNF-á) in serum and BALF were determined by enzyme linked immunosorbent assay (ELISA). The total protein in BALF was measured by bicinchoninic acid methods, and the infiltrated cells in BALF were counted. The mRNA expressions and protein levels of microtube associated light chain 3B (LC3B), mitochondrial DNA coded cytochrome C oxidase IV (COX-IV) and nuclear factor-KB p65 (NF-KB p65) in lung tissues were determined by real-time fluorescent quantitation reverse transcription-polymerase chain reaction (RT-qPCR) and Western Blot.RESULTS: The histopathology of lung tissue and the ultra structure of AEC II were normal in CON group and NVT group, and the obvious inflammatory changes and pathological injury were found in HVT group. Compared with CON and NVT groups, the W/D ratio in HVT group was significantly increased (8.53±1.05 vs. 5.12±0.65, 5.57±0.55, both P < 0.05), and total protein, infiltrated cells, and IL-1â, IL-6 and TNF-á in BALF were significantly increased [total protein (g/L): 2.35±0.45 vs. 1.46±0.34, 1.76±0.51; infiltrated cells (?105/mL): 2.05±0.48 vs. 0.40±0.08, 0.60±0.23; IL-1â (ng/L): 119.82±6.56 vs. 76.15±3.32, 79.39±4.44; IL-6 (ìg/L): 4.10±0.52 vs. 1.97±0.40, 2.27±0.36; TNF-á (mg/L): 1.49±0.28 vs. 0.43±0.23, 0.61±0.24; all P < 0.05], IL-1â, IL-6 and TNF-á levels in serum were also elevated [IL-1â (ng/L): 127.53±7.10 vs. 79.40±2.80, 82.95±2.25; IL-6 (ìg/L): 6.28±0.82 vs. 2.96±0.35, 3.36±0.72; TNF-á (mg/L): 1.59±0.42 vs. 0.53±0.22, 0.78±0.25; all P < 0.05]. The mRNA expressions and protein levels of LC3B, COX-IV and NF-KB p65 in lung tissue of HVT group were significantly higher than those of CON group and NVT group, the mRNA expressions of LC3B-II, COX-IV and NF-KB p65 were (3.52±0.90), (3.76±1.16) and (9.54±2.06) folds of those in CON group, and the protein expressions were (1.76±0.24), (1.65±0.20) and (1.91±0.12) folds of those in CON group, with significantly statistical differences (all P < 0.05). There were no significant differences in the parameters mentioned above between NVT group and CON group.CONCLUSIONS: Mitophagy may be associated with VILI resulting from escaped mitochondrial DNA for activation of inflammation.
|
['Animals', 'Bronchoalveolar Lavage Fluid', 'Inflammation', 'Interleukin-1beta', 'Interleukin-6', 'Lung', 'Mitophagy', 'NF-kappa B', 'Rats', 'Rats, Sprague-Dawley', 'Respiration, Artificial', 'Tidal Volume', 'Tumor Necrosis Factor-alpha', 'Ventilator-Induced Lung Injury']
| 28,459,395
|
[['B01.050'], ['E05.927.100.500'], ['C23.550.470'], ['D12.644.276.374.465.010.600', 'D12.644.276.374.500.400.600', 'D12.776.467.374.465.010.600', 'D12.776.467.374.500.400.600', 'D23.529.374.465.131.600', 'D23.529.374.500.400.600'], ['D12.644.276.374.465.224', 'D12.776.467.374.465.202', 'D23.529.374.465.224'], ['A04.411'], ['G04.011.937', 'G04.599.500'], ['D05.500.672', 'D12.776.260.600', 'D12.776.660.600', 'D12.776.930.600'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.750'], ['E02.041.625', 'E02.365.647.729', 'E02.880.820'], ['E01.370.386.700.485.750.900.350.750', 'G09.772.850.970.500.700'], ['D12.644.276.374.500.800', 'D12.644.276.374.750.626', 'D12.776.124.900', 'D12.776.395.930', 'D12.776.467.374.500.800', 'D12.776.467.374.750.626', 'D23.529.374.500.800', 'D23.529.374.750.626'], ['C08.381.520.750']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Cellular interplay via cytokine hierarchy causes pathological cardiac hypertrophy in RAF1-mutant Noonan syndrome.
|
Noonan syndrome (NS) is caused by mutations in RAS/ERK pathway genes, and is characterized by craniofacial, growth, cognitive and cardiac defects. NS patients with kinase-activating RAF1 alleles typically develop pathological left ventricular hypertrophy (LVH), which is reproduced in Raf1L613V/+ knock-in mice. Here, using inducible Raf1L613V expression, we show that LVH results from the interplay of cardiac cell types. Cardiomyocyte Raf1L613V enhances Ca2+ sensitivity and cardiac contractility without causing hypertrophy. Raf1L613V expression in cardiomyocytes or activated fibroblasts exacerbates pressure overload-evoked fibrosis. Endothelial/endocardial (EC) Raf1L613V causes cardiac hypertrophy without affecting contractility. Co-culture and neutralizing antibody experiments reveal a cytokine (TNF/IL6) hierarchy in Raf1L613V-expressing ECs that drives cardiomyocyte hypertrophy in vitro. Furthermore, postnatal TNF inhibition normalizes the increased wall thickness and cardiomyocyte hypertrophy in vivo. We conclude that NS-cardiomyopathy involves cardiomyocytes, ECs and fibroblasts, TNF/IL6 signalling components represent potential therapeutic targets, and abnormal EC signalling might contribute to other forms of LVH.
|
['Alleles', 'Animals', 'Coculture Techniques', 'Cytokines', 'Disease Models, Animal', 'Fibroblasts', 'Fibrosis', 'Gene Knock-In Techniques', 'Humans', 'Hypertrophy, Left Ventricular', 'Interleukin-6', 'MAP Kinase Signaling System', 'Male', 'Mice', 'Mice, Transgenic', 'Mutation', 'Myocardium', 'Myocytes, Cardiac', 'Noonan Syndrome', 'Primary Cell Culture', 'Proto-Oncogene Proteins c-raf', 'Tumor Necrosis Factor-alpha']
| 28,548,091
|
[['G05.360.340.024.340.030'], ['B01.050'], ['E05.481.500.374'], ['D12.644.276.374', 'D12.776.467.374', 'D23.529.374'], ['C22.232', 'E05.598.500', 'E05.599.395.080'], ['A11.329.228'], ['C23.550.355'], ['E05.393.335.249'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C14.280.195.400', 'C23.300.775.250.400'], ['D12.644.276.374.465.224', 'D12.776.467.374.465.202', 'D23.529.374.465.224'], ['G02.111.820.560', 'G03.493.560', 'G04.835.560'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.136.500', 'B01.050.150.900.649.313.992.635.505.500.800'], ['G05.365.590'], ['A02.633.580', 'A07.541.704', 'A10.690.552.750'], ['A07.541.704.570', 'A10.690.552.750.570', 'A11.620.500'], ['C05.660.207.690', 'C14.240.400.787', 'C14.280.400.787', 'C16.131.240.400.784', 'C16.131.621.207.690', 'C17.300.690'], ['E01.370.225.500.223.500', 'E05.200.500.265.500', 'E05.242.223.500', 'E05.481.500.249.500'], ['D08.811.913.696.620.682.700.559.842.500', 'D12.644.360.400.842.500', 'D12.776.476.400.842.500', 'D12.776.624.664.700.204.500'], ['D12.644.276.374.500.800', 'D12.644.276.374.750.626', 'D12.776.124.900', 'D12.776.395.930', 'D12.776.467.374.500.800', 'D12.776.467.374.750.626', 'D23.529.374.500.800', 'D23.529.374.750.626']]
|
['Phenomena and Processes [G]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Assessment of Depression and Anxiety in Breast Cancer Patients: Prevalence and Associated Factors
|
Introduction: Having breast cancer or receiving treatment has been seen as a traumatic experience for women due
to its impacts on their self-image and sexual relationship, and may lead to an psychological reactions such as denial,
anger, or intense fear toward their disease and treatment process. Also many of breast cancer patients have psychiatric
morbidities such as depression and anxiety. Purpose: The purpose of this study was to assess the prevalence and
associated factors of depression and anxiety in breast cancer patients, in order to identify independent predictors of mental
health disorders risk. Material and Methods: A cohort of 152 breast cancer patients who were attending an outpatient
oncology department was recruited. Data were collected with a structured questionnaire consisted by social, clinical
and demographic information and PHQ-2 and GAD-2 scales. Results: The mean age of the patients was 53.25 years
(SD=12.10), 69.7% of the patients underwent mastectomy and 30.3% ongectomy. Chemotherapy received 46.1% of
patients as adjuvant therapy, 15.8% radiotherapy and 38.2% received both chemotherapy and radiotherapy. A large
percentage found to be classified as depressed (38.2%) and anxious (32.2%) and factors that found to be associated
were age, marital status, educational level, stage of cancer from univariate analyses and place of residence, religion,
symptoms burden from multivariate analysis (for depression and anxiety). Conclusions: Breast cancer patients are
in high risk for developing psychiatric disorders such as depression and anxiety. Being rural resident, non-Orthodox
Christian and experiencing extend symptom burden can be predicting factors associated with depression and anxiety
in breast cancer patients.
|
['Adult', 'Aged', 'Anxiety Disorders', 'Breast Neoplasms', 'Cohort Studies', 'Combined Modality Therapy', 'Cross-Sectional Studies', 'Depressive Disorder', 'Female', 'Follow-Up Studies', 'Greece', 'Humans', 'Middle Aged', 'Prevalence', 'Prognosis', 'Stress, Psychological']
| 29,938,451
|
[['M01.060.116'], ['M01.060.116.100'], ['F03.080'], ['C04.588.180', 'C17.800.090.500'], ['E05.318.372.500.750', 'N05.715.360.330.500.750', 'N06.850.520.450.500.750'], ['E02.186'], ['E05.318.372.500.875', 'N05.715.360.330.500.875', 'N06.850.520.450.500.875'], ['F03.600.300'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['Z01.542.383'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['E05.318.308.985.525.750', 'N01.224.935.597.750', 'N06.850.505.400.975.525.750', 'N06.850.520.308.985.525.750'], ['E01.789'], ['F01.145.126.990', 'F02.830.900']]
|
['Named Groups [M]', 'Psychiatry and Psychology [F]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Geographicals [Z]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Chasing the Patagonian sun: comparative thermal biology of Liolaemus lizards.
|
The importance of the thermal environment for ectotherms and its relationship with thermal physiology and ecology is widely recognized. Several models have been proposed to explain the evolution of the thermal biology of ectotherms, but experimental studies have provided mixed support. Lizards from the Liolaemus goetschi group can be found along a wide latitudinal range across Argentina. The group is monophyletic and widely distributed, and therefore provides excellent opportunities to study the evolution of thermal biology. We studied thermal variables of 13 species of the L. goetschi group, in order to answer three questions. First, are aspects of the thermal biology of the L. goetschi group modelled by the environment or are they evolutionarily conservative? Second, have thermal characteristics of these animals co-evolved? And third, how do the patterns of co-evolution observed within the L. goetschi group compare to those in a taxonomically wider selection of species of Liolaemus? We collected data on 13 focal species and used species information of Liolaemus lizards available in the literature and additional data obtained by the authors. We tackled these questions using both conventional and phylogenetically based analyses. Our results show that lizards from the L. goetschi group and the genus Liolaemus in general vary in critical thermal minimum in relation to mean air temperature, and particularly the L. goetschi group shows that air temperature is associated with critical thermal range, as well as with body temperature. Although the effect of phylogeny cannot be ignored, our results indicate that these thermal biology aspects are modelled by cold environments of Patagonia, while other aspects (preferred body temperature and critical thermal maximum) are more conservative. We found evidence of co-evolutionary patterns between critical thermal minimum and preferred body temperature at both phylogenetic scales (the L. goetschi group and the extended sample of 68 Liolaemus species).
|
['Acclimatization', 'Animals', 'Argentina', 'Biological Evolution', 'Body Temperature', 'Body Temperature Regulation', 'Computational Biology', 'Likelihood Functions', 'Lizards', 'Models, Biological', 'Models, Genetic', 'Phylogeny', 'Species Specificity', 'Temperature']
| 23,011,849
|
[['G07.025.133', 'G16.012.500.133'], ['B01.050'], ['Z01.107.757.077'], ['G05.045', 'G16.075'], ['E01.370.600.875.374', 'G07.110'], ['G07.110.232', 'G07.410.421', 'G16.012.500.535'], ['H01.158.273.180', 'L01.313.124'], ['E05.318.740.500.475', 'E05.318.740.600.400', 'E05.599.835.500', 'N05.715.360.750.530.450', 'N05.715.360.750.625.450', 'N06.850.520.830.500.475', 'N06.850.520.830.600.400'], ['B01.050.150.900.833.393'], ['E05.599.395'], ['E05.599.395.397'], ['G05.697', 'G16.075.605', 'L01.100.697'], ['G16.824'], ['G01.906.595', 'G16.500.275.063.725.710', 'G16.500.750.775.710', 'N06.230.150.450', 'N06.230.300.100.725.710']]
|
['Phenomena and Processes [G]', 'Organisms [B]', 'Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Information Science [L]', 'Health Care [N]']
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 1
|
Decreased renal perfusion after correction of experimental coarctation.
|
Hemodynamical changes in coarctation were studied with an experimental model. Coarctation of the thoracic aorta was induced in seven puppies at the age of 8 wk. After a 7-month follow-up period a corrective operation with a venous patch was performed. Two dogs were lost a few hours after the correction operation. The remaining five dogs were followed for 12 months postoperatively. Renal perfusion was measured with a 133Xenon washout method just prior to the operation, 1 h, 6 and 12 months postoperatively. The glomerular filtration rate was measured using the 51Cr-EDTA method 1 wk before the operation, 3 wk, 2, 6, and 12 months after it. Six healthy adult dogs were used as controls for glomerular filtration rate measurements. Peripheral renin activity was measured at operation, just before the correction of aortic coarctation, 1/2, 1 h, 1, 3, 7 days, 2 and 6 months after the correction operation. Renal perfusion decreased significantly (p less than 0.05) immediately after the correction operation and rose again during the follow-up. Peripheral renin activity rose significantly (p less than 0.01) from the preoperative values and was at its greatest 1 day after the operation. Later on, peripheral renin activity values returned to normal. Coarctated dogs had significantly (p less than 0.01) lower glomerular filtration rate values than controls in each measurement except at the 2 months postoperative measurement. These results support decreased renal perfusion with resultant increased peripheral renin activity as part of the pathomechanism of the paradoxical hypertension observed after correction of coarctation.
|
['Animals', 'Aorta, Thoracic', 'Aortic Coarctation', 'Disease Models, Animal', 'Dogs', 'Follow-Up Studies', 'Glomerular Filtration Rate', 'Hypertension', 'Ligation', 'Renal Circulation', 'Renin', 'Reoperation', 'Surgical Flaps']
| 3,317,256
|
[['B01.050'], ['A07.015.114.056.372'], ['C14.240.400.090', 'C14.280.400.090', 'C16.131.240.400.090'], ['C22.232', 'E05.598.500', 'E05.599.395.080'], ['B01.050.150.900.649.313.750.250.216.200'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['E01.370.390.400.300', 'G08.852.357'], ['C14.907.489'], ['E04.426'], ['G08.852.725', 'G09.330.100.812'], ['D08.811.277.656.074.500.780', 'D08.811.277.656.300.048.780', 'D08.811.277.656.837.750'], ['E04.690'], ['A10.850.710', 'E07.862.710']]
|
['Organisms [B]', 'Anatomy [A]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Profile of a leader: Caroline Wellwood: pragmatic visionary.
|
Caroline Wellwood is representative of a group of early Canadian nurses whose missionary ideals led them to innovative careers in other countries. Wellwood's visionary leadership laid the groundwork for the development of a modern system of nursing and nursing education in southwestern China.
|
['Canada', 'China', 'Christianity', 'Education, Nursing, Diploma Programs', 'History, 20th Century', 'Hospitals, Religious', 'International Educational Exchange', 'Missionaries', 'Nurse Administrators', 'Religious Missions', 'Schools, Nursing']
| 11,094,942
|
[['Z01.107.567.176'], ['Z01.252.474.164'], ['K01.844.188'], ['I02.358.462.482'], ['K01.400.504.968'], ['N02.278.421.481.600'], ['I01.615.500.650', 'I02.581'], ['M01.427'], ['M01.526.070.670', 'M01.526.485.650.580', 'N02.360.650.580'], ['K01.844.777', 'N03.540.297.500'], ['I02.783.495.623']]
|
['Geographicals [Z]', 'Humanities [K]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]', 'Named Groups [M]']
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 1
|
Caveolin-1 Controls Hyperresponsiveness to Mechanical Stimuli and Fibrogenesis-Associated RUNX2 Activation in Keloid Fibroblasts.
|
Keloids are pathological scars characterized by excessive extracellular matrix production that are prone to form in body sites with increased skin tension. CAV1, the principal coat protein of caveolae, has been associated with the regulation of cell mechanics, including cell softening and loss of stiffness sensing ability in NIH3T3 fibroblasts. Although CAV1 is present in low amounts in keloid fibroblasts (KFs), the causal association between CAV1 down-regulation and its aberrant responses to mechanical stimuli remain unclear. In this study, atomic force microscopy showed that KFs were softer than normal fibroblasts with a loss of stiffness sensing. The decrease of CAV1 contributed to the hyperactivation of fibrogenesis-associated RUNX2, a transcription factor germane to osteogenesis/chondrogenesis, and increased migratory ability in KFs. Treatment of KFs with trichostatin A, which increased the acetylation level of histone H3, increased CAV1 and decreased RUNX2 and fibronectin. Trichostatin A treatment also resulted in cell stiffening and decreased migratory ability in KFs. Collectively, these results suggest a role for CAV1 down-regulation in linking the aberrant responsiveness to mechanical stimulation and extracellular matrix accumulation with the progression of keloids, findings that may lead to new developments in the prevention and treatment of keloid scarring.
|
['Adult', 'Biopsy', 'Caveolin 1', 'Cell Movement', 'Cells, Cultured', 'Core Binding Factor Alpha 1 Subunit', 'Down-Regulation', 'Female', 'Fibroblasts', 'Gene Knockdown Techniques', 'Humans', 'Hydroxamic Acids', 'Keloid', 'Male', 'Microscopy, Atomic Force', 'Middle Aged', 'Primary Cell Culture', 'RNA, Small Interfering', 'Skin', 'Young Adult']
| 28,899,682
|
[['M01.060.116'], ['E01.370.225.500.384.100', 'E01.370.225.998.054', 'E01.370.388.100', 'E04.074', 'E05.200.500.384.100', 'E05.200.998.054', 'E05.242.384.100'], ['D12.644.360.024.264', 'D12.776.157.057.010', 'D12.776.476.024.280', 'D12.776.543.990.100.500', 'D12.776.744.287'], ['G04.198', 'G07.568.500.180'], ['A11.251'], ['D12.776.930.155.200.100'], ['G02.111.240', 'G05.308.200', 'G07.690.773.937'], ['A11.329.228'], ['E05.393.335.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D02.092.570.394', 'D02.241.511.372'], ['A10.165.450.300.425', 'C17.300.200.425', 'C23.550.355.274.510'], ['E01.370.350.515.666.400', 'E05.595.666.400'], ['M01.060.116.630'], ['E01.370.225.500.223.500', 'E05.200.500.265.500', 'E05.242.223.500', 'E05.481.500.249.500'], ['D13.150.650.700', 'D13.444.735.150.700', 'D13.444.735.790.552.875'], ['A17.815'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Organisms [B]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Neutrophil to Lymphocyte Ratio and Cardiovascular Disease Incidence in HIV-Infected Patients: A Population-Based Cohort Study.
|
Neutrophil to lymphocyte ratio (NLR) has been shown to predict occurrence of cardiovascular events in the general population. The aim of our study was to evaluate the role of NLR to predict major cardiovascular disease (CVD) events in HIV-infected subjects. We performed a retrospective cohort study of HIV-infected patients residing in the Local Health Authority (LHA) of Brescia, northern Italy, from 2000 to 2012. The incidence of CVD events in HIV-positive patients was compared with that expected in the general population living in the same area, computing standardized incidence ratios (SIRs). To evaluate the predictive role of NLR, univariate and multivariate Cox regression models were applied, computing hazard ratios (HRs). A total of 3766 HIV-infected patients (mean age 38.1 years, 71.3% males) were included (person-years 28768.6). A total of 134 CVD events occurred in 119 HIV-infected patients. A 2-fold increased risk (SIR 2.02) of CVD was found in HIV-infected patients compared to the general population. NLR levels measured at baseline and during follow-up were independently associated with CVD incidence, when also adjusting for both traditional CVD risk factors and HIV-related factors (HR 3.05 for NLR? 1.2). The area under the receiver operating characteristics (ROC) curve showed a modest, not statistically significant, increase, from 0.81 to 0.83, with addition of NLR to Framingham risk score model covariates. In conclusion an elevated NLR is a predictor of risk CVD in HIV-infected patients, independently from the traditional CVD risk factors.
|
['Adult', 'Cardiovascular Diseases', 'Case-Control Studies', 'Female', 'HIV Infections', 'Humans', 'Incidence', 'Italy', 'Lymphocyte Count', 'Male', 'Middle Aged', 'Neutrophils', 'ROC Curve', 'Retrospective Studies', 'Risk Factors']
| 27,148,878
|
[['M01.060.116'], ['C14'], ['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['C01.221.250.875', 'C01.221.812.640.400', 'C01.778.640.400', 'C01.925.782.815.616.400', 'C01.925.813.400', 'C20.673.480'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.308.985.525.375', 'N01.224.935.597.500', 'N06.850.505.400.975.525.375', 'N06.850.520.308.985.525.375'], ['Z01.542.489'], ['E01.370.225.500.195.107.595.500', 'E01.370.225.625.107.595.500', 'E05.200.500.195.107.595.500', 'E05.200.625.107.595.500', 'E05.242.195.107.595.500', 'G04.140.107.595.500', 'G09.188.105.595.500'], ['M01.060.116.630'], ['A11.118.637.415.583', 'A11.627.340.583', 'A11.733.689', 'A15.145.229.637.415.583', 'A15.382.490.315.583', 'A15.382.680.689'], ['E05.318.370.800.750', 'E05.318.740.872.750', 'N05.715.360.325.700.680', 'N06.850.520.445.800.750'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725']]
|
['Named Groups [M]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Geographicals [Z]', 'Phenomena and Processes [G]', 'Anatomy [A]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Consultation-referral among physicians: practice and process.
|
Consultation-referral is a part of everyday family practice. Although the process is taken for granted, it is a complex phenomenon. Neither the practice nor the process always meet the expectations of the referring physician or the consultant, and the patient may be the worse because of this discrepany. Studies of the practice and the process support this view. A model of the process is elaborated which can be used for the teaching of medical students or residents and which the practicing physician may use to improve his/her consultation-referral practices.
|
['Canada', 'Family Practice', 'Follow-Up Studies', 'Humans', 'Interprofessional Relations', 'Referral and Consultation', 'United Kingdom', 'United States']
| 621,471
|
[['Z01.107.567.176'], ['H02.403.340.500'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F01.829.401.205'], ['N04.452.758.849'], ['Z01.542.363'], ['Z01.107.567.875']]
|
['Geographicals [Z]', 'Disciplines and Occupations [H]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Psychiatry and Psychology [F]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
|
Cation dependent O-methyltransferases from rice.
|
Two lower molecular mass OMT genes (ROMT-15 and -17) were cloned from rice and expressed in Escherichia coli as glutathione S-transferase fusion proteins. ROMT-15 and -17 metabolized caffeoyl-CoA, flavones and flavonols containing two vicinal hydroxyl groups, although they exhibited different substrate specificities. The position of methylation in both luteolin and quercetin was determined to be the 3' hydroxyl group and myricetin and tricetin were methylated not only at 3' but also at 5' hydroxyl groups. ROMT-15 and -17 are cation-dependent and mutation of the predicted metal binding sites resulted in the loss of the enzyme activity, indicating that the metal ion has a critical role in the enzymatic methylation.
|
['Acyl Coenzyme A', 'Binding Sites', 'Catalysis', 'Cations', 'Flavonoids', 'Gene Expression', 'Methylation', 'Methyltransferases', 'Molecular Weight', 'Oryza', 'Substrate Specificity']
| 17,943,312
|
[['D03.633.100.759.646.138.382.300', 'D08.211.211.300', 'D13.695.667.138.382.300', 'D13.695.827.068.382.300'], ['G02.111.570.120'], ['G02.130'], ['D01.248.497.300'], ['D03.383.663.283.266.450', 'D03.633.100.150.266.450'], ['G05.297'], ['G02.111.035.538', 'G02.607.094.538', 'G03.059.538'], ['D08.811.913.555.500'], ['G02.494'], ['B01.650.940.800.575.912.250.822.616'], ['G02.111.835']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
The tetratricopeptide repeat-domain of the PAS10 protein of Saccharomyces cerevisiae is essential for binding the peroxisomal targeting signal-SKL.
|
The PAS10 gene was found in a two-hybrid screen for the isolation of genes encoding proteins which interact with the C-terminal peroxisomal targeting signal -SKL. The PAS10 protein is known to be involved in import of proteins into peroxisomes and to contain a tetratricopeptide repeat (TPR) domain. All TPR-containing proteins involved in diverse processes like mitosis or RNA-synthesis share the ability to interact with other proteins. Here we show that the PAS10 protein interacts in vivo with the C-terminal peroxisomal targeting signal. The part essential for this interaction contains the complete tetratricopeptide repeat domain.
|
['Amino Acid Sequence', 'Base Sequence', 'Carrier Proteins', 'Fungal Proteins', 'Genes, Fungal', 'Membrane Transport Proteins', 'Microbodies', 'Molecular Sequence Data', 'Mutagenesis, Insertional', 'Peroxisome-Targeting Signal 1 Receptor', 'Plasmids', 'Protein Sorting Signals', 'Repetitive Sequences, Nucleic Acid', 'Saccharomyces cerevisiae', 'Saccharomyces cerevisiae Proteins']
| 7,980,572
|
[['G02.111.570.060', 'L01.453.245.667.060'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['D12.776.157'], ['D12.776.354'], ['G05.360.340.024.340.364.500', 'G05.360.340.358.024.500', 'G05.360.340.358.365.500'], ['D12.776.157.530', 'D12.776.543.585'], ['A11.284.430.214.190.500.585', 'A11.284.430.214.190.875.190.190.755'], ['L01.453.245.667'], ['E05.393.420.601.550', 'G05.365.590.575', 'G05.558.550'], ['D12.776.157.635.750'], ['G05.360.600'], ['D12.644.770', 'G02.111.570.060.670'], ['G02.111.570.080.708', 'G05.360.080.708'], ['B01.300.107.795.785.800', 'B01.300.930.705.655'], ['D12.776.354.750']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
CovR and VicRK regulate cell surface biogenesis genes required for biofilm formation in Streptococcus mutans.
|
The two-component system VicRK and the orphan regulator CovR of Streptococcus mutans co-regulate a group of virulence genes associated with the synthesis of and interaction with extracellular polysaccharides of the biofilm matrix. Knockout mutants of vicK and covR display abnormal cell division and morphology phenotypes, although the gene function defects involved are as yet unknown. Using transcriptomic comparisons between parent strain UA159 with vicK (UAvic) or covR (UAcov) deletion mutants together with electrophoretic motility shift assays (EMSA), we identified genes directly regulated by both VicR and CovR with putative functions in cell wall/surface biogenesis, including gbpB, wapE, smaA, SMU.2146c, and lysM. Deletion mutants of genes regulated by VicR and CovR (wapE, lysM, smaA), or regulated only by VicR (SMU.2146c) or CovR (epsC) promoted significant alterations in biofilm initiation, including increased fragility, defects in microcolony formation, and atypical cell morphology and/or chaining. Significant reductions in mureinolytic activity and/or increases in DNA release during growth were observed in knockout mutants of smaA, wapE, lysM, SMU.2146c and epsC, implying roles in cell wall biogenesis. WapE and lysM mutations also affected cell hydrophobicity and sensitivity to osmotic or oxidative stress. Finally, vicR, covR and VicRK/CovR-targets (gbpB, wapE, smaA, SMU.2146c, lysM, epsC) are up-regulated in UA159 during biofilm initiation, in a sucrose-dependent manner. These data support a model in which VicRK and CovR coordinate cell division and surface biogenesis with the extracellular synthesis of polysaccharides, a process apparently required for formation of structurally stable biofilms in the presence of sucrose.
|
['Bacterial Proteins', 'Biofilms', 'Gene Expression Profiling', 'Repressor Proteins', 'Streptococcus mutans', 'Sucrose']
| 23,554,881
|
[['D12.776.097'], ['A20.593', 'G06.120'], ['E05.393.332'], ['D12.776.260.703', 'D12.776.930.780'], ['B03.353.750.737.872.875.520', 'B03.510.400.800.872.875.520', 'B03.510.550.737.872.875.520'], ['D09.698.629.305.770', 'D09.947.750.770']]
|
['Chemicals and Drugs [D]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Multiple Roles for Egalitarian in Polarization of the Drosophila Egg Chamber.
|
The Drosophila egg chamber provides a useful model for examining mechanisms by which cell fates are specified and maintained in the context of a complex tissue. The egg chamber is also an excellent model for understanding the mechanism by which cytoskeletal filaments are organized and the critical interplay between cytoskeletal organization, polarity establishment, and cell fate specification. Previous work has shown that Egalitarian (Egl) is required for specification and maintenance of oocyte fate. Mutants in egl either completely fail to specify an oocyte, or if specified, the oocyte eventually reverts back to nurse cell fate. Due to this very early role for Egl in egg chamber maturation, it is unclear whether later stages of egg chamber development also require Egl function. In this report, we have depleted Egl at specific stages of egg chamber development. We demonstrate that in early-stage egg chambers, Egl has an additional role in organization of oocyte microtubules. In the absence of Egl function, oocyte microtubules completely fail to reorganize. As such, the localization of microtubule motors and their cargo is disrupted. In addition, Egl also appears to function in regulating the translation of critical polarity determining messenger RNAs (mRNAs). Finally, we demonstrate that in midstage egg chambers, Egl does not appear to be required for microtubule organization, but rather for the correct spatial localization of oskar, bicoid, and gurken mRNAs.
|
['Animals', 'Drosophila', 'Drosophila Proteins', 'Female', 'Microtubules', 'Oocytes', 'Oogenesis', 'RNA, Messenger']
| 27,017,624
|
[['B01.050'], ['B01.050.500.131.617.720.500.500.750.310.250'], ['D12.776.093.500.462'], ['A11.284.430.214.190.750.602'], ['A05.360.490.690.680', 'A11.497.497.600'], ['G04.152.650.249', 'G08.686.784.310.500'], ['D13.444.735.544']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Detection of designer steroid methylstenbolone in "nutritional supplement" using gas chromatography and tandem mass spectrometry: elucidation of its urinary metabolites.
|
The use of "nutritional supplements" containing unapproved substances has become a regular practice in amateur and professional athletes. This represents a dangerous habit for their health once no data about toxicological or pharmacological effects of these supplements are available. Most of them are freely commercialized online and any person can buy them without medical surveillance. Usually, the steroids intentionally added to the "nutritional supplements" are testosterone analogues with some structural modifications. In this study, the analyzed product was bought online and a new anabolic steroid known as methylstenbolone (2,17á-dimethyl-17â-hydroxy-5á-androst-1-en-3-one) was detected, as described on label. Generally, anabolic steroids are extensively metabolized, thus in-depth knowledge of their metabolism is mandatory for doping control purposes. For this reason, a human excretion study was carried out with four volunteers after a single oral dose to determine the urinary metabolites of the steroid. Urine samples were submitted to enzymatic hydrolysis of glucuconjugated metabolites followed by liquid-liquid extraction and analysis of the trimethylsilyl derivatives by gas chromatography coupled to tandem mass spectrometry. Mass spectrometric data allowed the proposal of two plausible metabolites: 2,17á-dimethyl-16î,17â-dihydroxy-5á-androst-1-en-3-one (S1), 2,17á-dimethyl-3á,16î,17â-trihydroxy-5á-androst-1-ene (S2). Their electron impact mass spectra are compatible with 16-hydroxylated steroids O-TMS derivatives presenting diagnostic ions such as m/z 231 and m/z 218. These metabolites were detectable after one week post administration while unchanged methylstenbolone was only detectable in a brief period of 45 h.
|
['Adult', 'Androstenols', 'Chromatography, Gas', 'Designer Drugs', 'Dietary Supplements', 'Humans', 'Kinetics', 'Male', 'Steroids', 'Tandem Mass Spectrometry', 'Time Factors']
| 23,200,734
|
[['M01.060.116'], ['D04.210.500.054.079.429'], ['E05.196.181.349'], ['D26.909.500'], ['G07.203.300.456', 'J02.500.456'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G01.374.661', 'G02.111.490'], ['D04.210.500'], ['E05.196.566.880'], ['G01.910.857']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Technology, Industry, and Agriculture [J]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
|
Association of transporter associated with antigen processing (TAP) gene polymorphisms in donors with acute cellular rejection in living donor liver transplantation.
|
BACKGROUND & AIMS: Despite improvements in immunosuppressive therapy, acute cellular rejection (ACR) remains an important cause of mortality and graft loss in patients undergoing liver transplantation. Recently, associations between gene polymorphisms and the incidence of ACR have been reported, though few studies have investigated those polymorphisms in donors. Transporter associated with antigen processing (TAP1 and TAP2) are involved in major histocompatibility complex (MHC) class I antigen-mediated processing and presentation to cytotoxic CD8+ T lymphocytes. The aim of this study was to determine whether TAP1 and TAP2 gene polymorphisms in the donor have affected on ACR incidence in living donor liver transplantation (LDLT).METHODS: We examined 155 LDLTs treated at Nagoya University or Kyoto University from 2004 to 2009 and analyzed the gene polymorphisms of TAP-1 p.Ile333Val, TAP-1 p.Asp697Gly, TAP-2 p.Arg651Cys, and TAP-2 p.Gln687Stop.RESULTS: Thirty-seven recipients developed early ACR. Of the investigated gene polymorphisms, the TAP-1 p.697Gly allele in donors was associated with incidence of early ACR (OR=2.97, 95%CI 1.33-6.63, p=0.008).CONCLUSIONS: The TAP-1 p.697Gly allele in donors was associated with increased incidence of early ACR following LDLT. The TAP-1 697 polymorphism in donors can be genotyped prior to LDLT, which may contribute to individualize immunosuppression strategies for recipients and donor selection.
|
['ATP Binding Cassette Transporter, Subfamily B, Member 2', 'ATP Binding Cassette Transporter, Subfamily B, Member 3', 'ATP-Binding Cassette Transporters', 'Acute Disease', 'Adolescent', 'Adult', 'Aged', 'Chi-Square Distribution', 'Child', 'Child, Preschool', 'Female', 'Gene Frequency', 'Genetic Predisposition to Disease', 'Graft Rejection', 'Humans', 'Immunity, Cellular', 'Immunosuppressive Agents', 'Incidence', 'Infant', 'Japan', 'Liver Transplantation', 'Living Donors', 'Logistic Models', 'Male', 'Middle Aged', 'Odds Ratio', 'Phenotype', 'Polymorphism, Genetic', 'Risk Factors', 'Treatment Outcome', 'Young Adult']
| 23,799,215
|
[['D12.776.157.530.100.075.250', 'D12.776.157.530.450.074.500.500.250.250', 'D12.776.395.550.020.400.305', 'D12.776.543.550.192.400.305', 'D12.776.543.585.100.200.250', 'D12.776.543.585.450.074.500.500.250.250'], ['D12.776.157.530.100.075.500', 'D12.776.157.530.450.074.500.500.250.375', 'D12.776.395.550.020.400.458', 'D12.776.543.550.192.400.458', 'D12.776.543.585.100.200.375', 'D12.776.543.585.450.074.500.500.250.375'], ['D12.776.157.530.100', 'D12.776.395.550.020', 'D12.776.543.550.192', 'D12.776.543.585.100'], ['C23.550.291.125'], ['M01.060.057'], ['M01.060.116'], ['M01.060.116.100'], ['E05.318.740.994.300', 'G17.820.300', 'N05.715.360.750.750.200', 'N06.850.520.830.994.300'], ['M01.060.406'], ['M01.060.406.448'], ['G05.330'], ['C23.550.291.687.500', 'G05.380.355'], ['G12.875.545.328'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G12.450.050.400'], ['D27.505.696.477.656'], ['E05.318.308.985.525.375', 'N01.224.935.597.500', 'N06.850.505.400.975.525.375', 'N06.850.520.308.985.525.375'], ['M01.060.703'], ['Z01.252.474.463', 'Z01.639.595'], ['E02.095.147.725.490', 'E04.210.650', 'E04.936.450.490', 'E04.936.580.490'], ['M01.898.656'], ['E05.318.740.500.525', 'E05.318.740.600.800.450', 'E05.318.740.750.450', 'E05.599.835.875', 'N05.715.360.750.530.480', 'N05.715.360.750.625.700.450', 'N05.715.360.750.695.470', 'N06.850.520.830.500.525', 'N06.850.520.830.600.800.450', 'N06.850.520.830.750.450'], ['M01.060.116.630'], ['E05.318.740.600.600', 'G17.680.500', 'N05.715.360.750.625.590', 'N06.850.520.830.600.600'], ['G05.695'], ['G05.365.795'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['M01.060.116.815']]
|
['Chemicals and Drugs [D]', 'Diseases [C]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Organisms [B]', 'Geographicals [Z]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Predictors of cardiovascular response to smoked cocaine in humans.
|
The purpose of this study was to examine the predictors of heart rate and blood pressure changes following cocaine administration. Sixty-two smoked cocaine users received a single 0.4 mg/kg dose of smoked cocaine. Male sex, African American race, higher body weight and current marijuana use predicted a greater cardiovascular response to cocaine. In contrast, higher baseline blood pressure, heart rate, amount and frequency of current cocaine use and presence of current cocaine snorting predicted a diminished cardiovascular response to cocaine. Whether these predictors of the cardiovascular response to smoked cocaine in the laboratory also predict cardiovascular complications from long-term cocaine use needs to be studied further.
|
['Administration, Inhalation', 'Adult', 'Arousal', 'Blood Pressure', 'Cocaine-Related Disorders', 'Crack Cocaine', 'Female', 'Heart Rate', 'Humans', 'Male']
| 10,661,674
|
[['E02.319.267.050'], ['M01.060.116'], ['F02.830.104', 'G11.561.035'], ['E01.370.600.875.249', 'G09.330.380.076'], ['C25.775.300', 'F03.900.300'], ['D02.145.074.722.388.250', 'D03.132.889.354.250', 'D03.605.084.500.722.388.250', 'D03.605.869.388.250', 'D26.878.250'], ['E01.370.600.875.500', 'G09.330.380.500'], ['B01.050.150.900.649.313.988.400.112.400.400']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Named Groups [M]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
POMC mRNA levels in individual melanotropes and GFAP in glial-like cells in rat pituitary.
|
In this study we demonstrated that individual melanotropes expressed differing levels of POMC mRNA. Interspersed among the melanotropes was a small population of process-bearing cells expressing GFAP. We compared cellular resolution and feasibility for quantitation of hybrids formed by oligonucleotide probes labeled with two different markers using in situ hybridization histochemistry. In hybridizations using [35S]-labeled probes the signal could not easily be assigned to individual melanotropes, although digoxigenin-labeled probes provided good cellular resolution. Heterogeneous staining intensities of individual melanotropes for POMC mRNA were measured, and relative quantitation of changes in POMC mRNA levels following interactions with the D2 dopamine receptor was performed. We combined in situ hybridization histochemistry with immunohistochemistry to demonstrate that cells within the lobe parenchyma not expressing POMC mRNA were immunoreactive for GFAP.
|
['Animals', 'Base Sequence', 'Bromocriptine', 'Glial Fibrillary Acidic Protein', 'Haloperidol', 'Histocytochemistry', 'Image Processing, Computer-Assisted', 'Immunohistochemistry', 'In Situ Hybridization', 'Male', 'Melanins', 'Molecular Sequence Data', 'Neuroglia', 'Pituitary Gland', 'Pro-Opiomelanocortin', 'RNA, Messenger', 'Rats', 'Rats, Sprague-Dawley']
| 8,008,629
|
[['B01.050'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['D03.132.327.412.100', 'D03.633.400.439.131', 'D03.633.400.562.100'], ['D05.750.078.593.400', 'D12.776.220.475.400'], ['D02.522.352.506'], ['E01.370.225.500.607', 'E01.370.225.750.551', 'E05.200.500.607', 'E05.200.750.551', 'H01.158.100.656.234', 'H01.158.201.344', 'H01.181.122.573'], ['L01.224.308'], ['E01.370.225.500.607.512', 'E01.370.225.750.551.512', 'E05.200.500.607.512', 'E05.200.750.551.512', 'E05.478.583', 'H01.158.100.656.234.512', 'H01.158.201.344.512', 'H01.158.201.486.512', 'H01.181.122.573.512', 'H01.181.122.605.512'], ['E01.370.225.500.620.670.325', 'E01.370.225.750.600.670.325', 'E05.200.500.620.670.325', 'E05.200.750.600.670.325', 'E05.393.661.475'], ['D12.125.072.050.875.379', 'D23.767.620'], ['L01.453.245.667'], ['A08.637', 'A11.650'], ['A06.300.747', 'A06.688.357.750', 'A08.186.211.180.497.352.435.500', 'A08.186.211.200.317.357.352.435.500', 'A08.713.357.750'], ['D06.472.699.327.935', 'D06.472.699.631.525.600', 'D12.644.400.400.935', 'D12.644.548.365.935', 'D12.644.548.691.525.690', 'D12.776.631.650.405.935', 'D12.776.811.800'], ['D13.444.735.544'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.750']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
|
Analysis of vasoreactivity of isolated human radial artery.
|
Radial artery (RA) is increasingly used as graft for coronary artery bypass grafting due to its good long-term patency. However, the mechanism of peri- and post-operative spasm is still unclear. Because of that, the aim of our study is to analyze the contractility of RA and to determine whether the presence of functional endothelium alters its contractile properties. Contractions of isolated RA rings were provoked by exogenously applied vasoconstrictors or by electrical field stimulation (EFS, 20 Hz). The order of vasoconstrictors potency based on their EC50 values was as follows: angiotensin II > phenylephrine > 5-hydroxytriptamine. Presence of endothelium increased both EC50 and maximal contraction to phenylephrine and angiotensin II, but inhibited reactivity of RA to 5-hydroxytriptamine. Spontaneous rhythmic contractions (SRC, <4 mHz) and EFS-induced contractions of RA are endothelium-independent and weaker than contractions induced by exogenously applied vasoconstrictors. Our study concludes that RA shows marked sensitivity and reactivity to angiotensin II, phenylephrine, and 5-hydroxytriptamine. Further investigations are necessary to answer why angiotensin II and phenylepehrine induce stronger contractions in the presence of endothelium. In addition, SRC as well as contractions of neurogenic origin may take part in developing vascular spasm of RA.
|
['Angiotensin II', 'Dose-Response Relationship, Drug', 'Electric Stimulation', 'Endothelium, Vascular', 'Humans', 'In Vitro Techniques', 'Muscle, Smooth, Vascular', 'Phenylephrine', 'Radial Artery', 'Serotonin', 'Time Factors', 'Vasoconstriction', 'Vasoconstrictor Agents']
| 16,404,133
|
[['D06.472.699.094.078', 'D12.644.400.070.078', 'D12.644.456.073.041', 'D12.644.548.058.078', 'D12.776.631.650.070.078', 'D23.469.050.050.050'], ['G07.690.773.875', 'G07.690.936.500'], ['E05.723.402'], ['A07.015.700.500', 'A10.272.491.355'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.481'], ['A02.633.570.491', 'A07.015.733.500', 'A10.690.467.491'], ['D02.033.100.291.617', 'D02.092.063.291.617'], ['A07.015.114.740'], ['D02.092.211.215.801.852', 'D03.633.100.473.914.814', 'D23.469.050.650'], ['G01.910.857'], ['G09.330.380.925'], ['D27.505.954.411.793']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Organisms [B]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Essential regions for antiviral activities of actinohivin, a sugar-binding anti-human immunodeficiency virus protein from an actinomycete.
|
Actinohivin (AH) is a potent anti-human immunodeficiency virus (HIV) protein that consists of highly conserved three-tandem repeats (segments 1, 2, and 3). The molecular target of AH in its anti-HIV activity is high-mannose-type saccharide chains of HIV gp120. This article deals with sequence requirements for the anti-HIV activity of AH. The deleted or substituted DNAs encoding AH or His-AH were prepared using mutagenic oligonucleotide primers in PCR. The mutant constructs were expressed in Escherichia coli, and the activities of the recombinant protein products were examined by a syncytium-formation assay system that mimics anti-HIV activity. The single segment mutant His-AHs showed no anti-syncytium-formation activity, but the mutant His-AHs, which consists of 2 or 3 segments, retained reduced activities. His-AH(6-114) dramatically reduced the anti-syncytium-formation activity to that of His-AH(36-114) or His-AH(I5A). Furthermore, His-AH(Q33A), His-AH(Q71A), and His-AH(Q109A) in which glutamine residues were substituted into alanine showed reduced activities of 1/20, 1/10, and 1/30, respectively, in anti-syncytium formation compared with His-AH. These results indicate that three segments of AH are necessary for potent anti-syncytium-formation activity-that is, for potent anti-HIV activity and the cooperated involvement of each segment of AH increased the AH-gp120 interaction.
|
['Actinobacteria', 'Amino Acid Sequence', 'Amino Acid Substitution', 'Anti-HIV Agents', 'Bacterial Proteins', 'Carbohydrate Metabolism', 'Conserved Sequence', 'Cysteine', 'Enzyme-Linked Immunosorbent Assay', 'Giant Cells', 'HIV', 'HeLa Cells', 'Humans', 'Inhibitory Concentration 50', 'Molecular Sequence Data', 'Sequence Deletion']
| 15,850,563
|
[['B03.510.024', 'B03.510.460.400.400.049'], ['G02.111.570.060', 'L01.453.245.667.060'], ['E05.393.420.601.035', 'G05.558.109'], ['D27.505.954.122.388.077.088'], ['D12.776.097'], ['G02.111.158', 'G03.191'], ['G02.111.570.580'], ['D02.886.030.230', 'D02.886.489.155', 'D12.125.154.299', 'D12.125.166.230'], ['E05.478.566.350.170', 'E05.478.566.380.360', 'E05.478.583.400.170', 'E05.601.470.350.170', 'E05.601.470.380.360'], ['A11.500'], ['B04.820.650.589.650.350'], ['A11.251.210.190.400', 'A11.251.860.180.400', 'A11.436.340'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.940.350', 'G07.690.936.563'], ['L01.453.245.667'], ['G05.365.590.762', 'G05.558.800']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Inositol trisphosphate and diacylglycerol can differentially modulate gene expression in Dictyostelium.
|
We have previously shown that several genes expressed during Dictyostelium development could be induced in shaking culture by exogenous cAMP, even though the accumulation of intracellular cAMP was inhibited. The use of selected cAMP analogs indicated that the exogenous cAMP functioned by activating the cell surface cAMP receptor and not by interacting with the regulatory subunit of the intracellular cAMP-dependent protein kinase. Although some genes in Dictyostelium appear to be regulated by intracellular cAMP, these data suggest that this is not the case for all genes regulated by cAMP. Intracellular second messengers other than cAMP may, therefore, promote the expression of these other genes. Here, we have examined inositol trisphosphate and diacylglycerol as candidates for such mediators of signal transduction. We have studied three genes that exhibit disparate modes of temporal and spatial expression during development of Dictyostelium. In shaking cultures, maximal levels of expression of each are dependent on the accumulation of or exposure to extracellular cAMP. We show that the addition of inositol trisphosphate and/or diacylglycerol to cells in shaking culture has distinct effects on the expression of each gene and, under specific conditions, can bypass the requirement for extracellular cAMP. These data suggest that extracellular cAMP interacting with its cell surface receptor may promote synthesis of inositol trisphosphate and diacylglycerol to regulate gene expression and aspects of differentiation in Dictyostelium.
|
['Cyclic AMP', 'Dictyostelium', 'Diglycerides', 'Gene Expression', 'Gene Expression Regulation, Fungal', 'Genes, Fungal', 'Glycerides', 'Inositol 1,4,5-Trisphosphate', 'RNA, Fungal']
| 2,556,709
|
[['D03.633.100.759.646.138.395', 'D13.695.462.200', 'D13.695.667.138.395', 'D13.695.827.068.395'], ['B01.046.550.200.300'], ['D10.351.303'], ['G05.297'], ['G05.308.330'], ['G05.360.340.024.340.364.500', 'G05.360.340.358.024.500', 'G05.360.340.358.365.500'], ['D10.351'], ['D02.033.800.519.400.350', 'D09.853.519.400.350', 'D09.894.480.350'], ['D13.444.735.500']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Caspase-12 modulates NOD signaling and regulates antimicrobial peptide production and mucosal immunity.
|
Bacterial sensing by intracellular Nod proteins and other Nod-like receptors (NLRs) activates signaling pathways that mediate inflammation and pathogen clearance. Nod1 and Nod2 associate with the kinase Rip2 to stimulate NF-kappaB signaling. Other cytosolic NLRs assemble caspase-1-activating multiprotein complexes termed inflammasomes. Caspase-12 modulates the caspase-1 inflammasome, but unlike other NLRs, Nod1 and Nod2 have not been linked to caspases, and mechanisms regulating the Nod-Rip2 complex are less clear. We report that caspase-12 dampens mucosal immunity to bacterial infection independent of its effects on caspase-1. Caspase-12 deficiency enhances production of antimicrobial peptides, cytokines, and chemokines to entric pathogens, an effect dependent on bacterial type III secretion and the Nod pathway. Mechanistically, caspase-12 binds to Rip2, displacing Traf6 from the signaling complex, inhibiting its ubiquitin ligase activity, and blunting NF-kappaB activation. Nod activation and resulting antimicrobial peptide production constitute an early innate defense mechanism, and caspase-12 inhibits this mucosal antimicrobial response.
|
['Adaptor Proteins, Signal Transducing', 'Animals', 'Antimicrobial Cationic Peptides', 'Caspase 12', 'Citrobacter rodentium', 'Cytokines', 'Enterobacteriaceae Infections', 'Epithelial Cells', 'Female', 'Humans', 'Immunity, Mucosal', 'Mice', 'Mice, Inbred C57BL', 'Mice, Knockout', 'NF-kappa B', 'Nod1 Signaling Adaptor Protein', 'Nod2 Signaling Adaptor Protein', 'Protein Binding', 'Receptor-Interacting Protein Serine-Threonine Kinase 2', 'Receptor-Interacting Protein Serine-Threonine Kinases', 'TNF Receptor-Associated Factor 6']
| 18,329,614
|
[['D12.644.360.024', 'D12.776.157.057', 'D12.776.476.024'], ['B01.050'], ['D12.644.050', 'D12.776.543.695.054'], ['D08.811.277.656.262.500.126.550.920', 'D08.811.277.656.300.200.126.550.920', 'D12.644.360.075.405.550.920', 'D12.776.476.075.405.550.920'], ['B03.440.450.425.200.737', 'B03.660.250.150.100.737'], ['D12.644.276.374', 'D12.776.467.374', 'D23.529.374'], ['C01.150.252.400.310'], ['A11.436'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G12.450.573'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.420', 'B01.050.150.900.649.313.992.635.505.500.400.420'], ['B01.050.050.136.500.500', 'B01.050.150.900.649.313.992.635.505.500.550.455', 'B01.050.150.900.649.313.992.635.505.500.800.500'], ['D05.500.672', 'D12.776.260.600', 'D12.776.660.600', 'D12.776.930.600'], ['D12.644.360.024.131.249', 'D12.644.360.024.313.249', 'D12.644.360.075.358.249', 'D12.644.360.539.500.249', 'D12.776.157.057.006.249', 'D12.776.157.057.078.249', 'D12.776.476.024.139.249', 'D12.776.476.024.391.249', 'D12.776.476.075.358.249'], ['D12.644.360.024.131.500', 'D12.644.360.024.313.500', 'D12.644.360.075.358.500', 'D12.644.360.539.500.500', 'D12.776.157.057.006.500', 'D12.776.157.057.078.500', 'D12.776.476.024.139.500', 'D12.776.476.024.391.500', 'D12.776.476.075.358.500'], ['G02.111.679', 'G03.808'], ['D08.811.913.696.620.682.700.801.500', 'D12.644.360.024.131.750', 'D12.644.360.024.500.186.500', 'D12.644.360.075.358.750', 'D12.776.157.057.006.750', 'D12.776.157.057.500.186.500', 'D12.776.476.024.139.750', 'D12.776.476.024.500.186.500', 'D12.776.476.075.358.750'], ['D08.811.913.696.620.682.700.801', 'D12.644.360.024.285.400', 'D12.644.360.024.500.186', 'D12.644.360.075.421.400', 'D12.776.157.057.018.400', 'D12.776.157.057.500.186', 'D12.776.476.024.320.662', 'D12.776.476.024.500.186', 'D12.776.476.075.421.400'], ['D12.644.360.024.500.968', 'D12.776.157.057.500.968', 'D12.776.476.024.500.968']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Diseases [C]', 'Anatomy [A]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Molecular cloning and functional expression of the bumetanide-sensitive Na-K-Cl cotransporter.
|
By mediating the coupled movement of Na, K, and Cl ions across the plasma membrane of most animal cells, the bumetanide-sensitive Na-K-Cl cotransporter (NKCC) plays a vital role in the regulation of ionic balance and cell volume. The transporter is a central element in the process of vectorial salt transport in secretory and absorptive epithelia. A cDNA encoding a Na-K-Cl cotransport protein was isolated from a shark rectal gland library by screening with monoclonal antibodies to the native shark cotransporter. The 1191-residue protein predicted from the cDNA sequence has 12 putative transmembrane domains flanked by large cytoplasmic N and C termini. Regulatory phosphoacceptor residues in isolated peptides are identified as Thr-189 and Thr-1114 in the predicted sequence. Northern blot analysis identified a 7.4-kb mRNA in rectal gland and most other shark tissues; a 5.2-kb mRNA was restricted to shark kidney. Homology with an uncharacterized gene from Caenorhabditis elegans and with the thiazide-sensitive Na-Cl cotransporter of flounder urinary bladder was found over most of the coding region; shorter stretches of homology were found with a C. elegans cDNA and with an uncharacterized gene of cyanobacterium. Human HEK-293 cells have been stably transfected with the shark cDNA and shown to express Na-K-Cl cotransport activity with the bumetanide sensitivity of the shark protein. The expressed transporter is functionally quiescent in the host cells and can be activated by depleting the cells of chloride.
|
['Amino Acid Sequence', 'Animals', 'Base Sequence', 'Blotting, Northern', 'Bumetanide', 'Carrier Proteins', 'Cell Line', 'Chlorides', 'Cloning, Molecular', 'DNA', 'Gene Expression Regulation', 'Humans', 'Molecular Sequence Data', 'Osmolar Concentration', 'Potassium', 'Sharks', 'Sodium', 'Sodium-Potassium-Chloride Symporters']
| 8,134,373
|
[['G02.111.570.060', 'L01.453.245.667.060'], ['B01.050'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['E05.196.401.095', 'E05.301.300.074', 'E05.601.100'], ['D02.065.884.150', 'D02.241.223.100.050.300.200', 'D02.455.426.559.389.127.020.452.500', 'D02.886.590.700.150'], ['D12.776.157'], ['A11.251.210'], ['D01.210.450.150', 'D01.248.497.158.215'], ['E05.393.220'], ['D13.444.308'], ['G05.308'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['L01.453.245.667'], ['G02.640'], ['D01.268.549.550', 'D01.268.557.575', 'D01.552.528.652', 'D01.552.547.650'], ['B01.050.150.900.493.370.853'], ['D01.268.549.750', 'D01.268.557.650', 'D01.552.528.850', 'D01.552.547.725'], ['D12.776.157.530.450.625.750', 'D12.776.157.530.937.750', 'D12.776.543.585.450.625.750', 'D12.776.543.585.937.875']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Light-induced vasodilation of coronary arteries and its possible clinical implication.
|
BACKGROUND: Low-level laser therapy and light-emitting diodes (LED) are increasingly used in phototherapy. Their therapeutic effects are at least partly mediated by light-induced vasodilation. The aim of this study was to determine the effect of different light sources on coronary arteries.METHODS: Porcine left coronary arteries were cut into 4-mm rings that were irradiated either by a semiconductor nonthermal gallium-arsenide diode laser or a noncoherent athermic red light source both with the same energy density up to 16 J/cm(2). After precontraction with 9, 11-dideoxy-11á, 9á-epoxymethano-prostaglandin F(2)á, respective relaxation responses were evaluated. The role of endothelium as well as intracellular pathways was investigated.RESULTS: Maximum vasodilation after exposure to laser was observed at 10 J/cm(2) (56.8% ± 1.2%) and decreased to 43.9% ± 2.8% at 16 J/cm(2) (p < 0.003). After adjusting exposure time to achieve equivalent energy densities in the LED group, vessel segments revealed photorelaxation of 52.9% ± 6.5% and 47.5% ± 0.6%, respectively. Vasodilations achieved by either light source were comparable at 10 J/cm(2) (p < 0.574) and 16 J/cm(2) (p < 0.322). Furthermore, vasodilation could be inhibited by administration of 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (nitric oxide scavenger) and 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (guanocyclase inhibitor) but not with L-nitro-arginine methyl ester or deendothelialization.CONCLUSIONS: Vessels exposed to either light source showed a remarkable as well as comparable photorelaxation at definite energy densities. This effect is mediated by an intracellular nitric oxide-dependent mechanism. As LED sources are of small size, simple, and inexpensive build-up, they may be used during routine coronary artery bypass surgery to ease suturing of anastomosis by target vessel vasodilation.
|
['Animals', 'Coronary Vessels', 'Light', 'Low-Level Light Therapy', 'Models, Animal', 'Phototherapy', 'Swine', 'Vasodilation']
| 22,381,453
|
[['B01.050'], ['A07.015.114.269', 'A07.015.908.194'], ['G01.358.500.505.650', 'G01.590.540', 'G01.750.250.650', 'G01.750.770.578'], ['E02.594.540', 'E02.774.500'], ['E05.598'], ['E02.774'], ['B01.050.150.900.649.313.500.880'], ['G09.330.380.928']]
|
['Organisms [B]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Different binding capacities of influenza A and Sendai viruses to gangliosides from human granulocytes.
|
The structures of gangliosides from human granulocytes were elucidated by fast atom bombardment mass spectrometry and by gas chromatography/mass spectrometry as their partially methylated alditol acetates. In human granulocytes besides GM3 (II3Neu5Ac-LacCer), neolacto-series gangliosides (IV3Neu5Ac-nLcOse4Cer, IV6Neu5Ac-nLcOse4Cer and VI3Neu5Ac-nLcOse6Cer) containing C24:1, and to some extent C22:0; and C16:0 fatty acid in their respective ceramide portions, were identified as major components. In this study we demonstrate that gangliosides from human granulocytes, the second most abundant cells in peripheral blood, can serve as receptors for influenza viruses A/PR/8/34 (H1N1), A/X-31 (H3N2), and a parainfluenza virus Sendai virus (HNF1, Z-strain). Viruses were found to exhibit specific adhesion to terminal Neu5Ac alpha 2-3Gal and/or Neu5Ac alpha 2-6Gal sequences as well as depending on the chain length of ganglioside carbohydrate backbones from human granulocytes, these important effector cells which represent the first line of defence in immunologically mediated reactions.
|
['Carbohydrate Sequence', 'Chromatography, Gas', 'Chromatography, Thin Layer', 'Gangliosides', 'Granulocytes', 'Humans', 'Influenza A virus', 'Molecular Sequence Data', 'Parainfluenza Virus 1, Human', 'Receptors, Virus', 'Spectrometry, Mass, Fast Atom Bombardment', 'Sulfur Radioisotopes']
| 8,395,268
|
[['G02.111.570.160', 'L01.453.245.667.160'], ['E05.196.181.349'], ['E05.196.181.400.537'], ['D09.400.410.420.025.475', 'D10.390.470.025.475', 'D10.570.877.360.025.475'], ['A11.118.637.415', 'A11.148.350', 'A11.627.340', 'A15.145.229.637.415', 'A15.378.316.340', 'A15.382.490.315'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['B04.820.480.968.405.400'], ['L01.453.245.667'], ['B04.820.480.937.600.650.700.715'], ['D12.776.543.750.830'], ['E05.196.566.750'], ['D01.268.185.900.500.690', 'D01.496.749.858', 'D01.496.868.690']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Organisms [B]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Bone tunnel enlargement after anterior cruciate ligament reconstruction using hamstring tendons.
|
We retrospectively reviewed 87 anterior cruciate ligament reconstructions using autogenous hamstring tendons with the Endobutton technique to investigate the relationship between bone tunnel enlargement and clinical outcome and to identify factors that contribute to the enlargement. The clinical outcome was evaluated using the Lysholm score and KT-1000 arthrometer. The location of the femoral tunnel with respect to Blumensaat's line, the tibial tunnel with respect to the tibial plateau, and the angle between the femoral tunnel and Blumensaat's line (femoral tunnel angle) were measured. Bone tunnel enlargement was observed in 32 patients (37%). Enlargement occurred in 22 of the femoral tunnels and 26 of the tibial tunnels. Enlargement of both tunnels occurred in 16 knees. There was no statistical difference in Lysholm scores or KT-1000 arthrometer measurements between the enlarged group and the unenlarged group. The femoral tunnel was placed more anteriorly in the enlarged femoral tunnel group than in the unenlarged femoral tunnel group. The tibial tunnel was placed more anteriorly in the enlarged tibial tunnel group than in the unenlarged tibial tunnel group. The femoral tunnel angle was significantly smaller in the enlarged femoral tunnel group than in the femoral unenlarged group. Gender, patient age, intraoperative isometricity, and graft size were not significant factors. Bone tunnel enlargement was not correlated with the clinical outcome measures. We conclude that the main factor associated with tunnel enlargement are the locations and angles of the tunnels. The windshield-wiper motion of the graft may be enhanced by changing tension in the graft due to tunnel malposition. An acute femoral tunnel angle may increase the mechanical stress on the anterior margin of the femoral tunnel.
|
['Adolescent', 'Adult', 'Anterior Cruciate Ligament Injuries', 'Biomechanical Phenomena', 'Female', 'Humans', 'Knee Injuries', 'Male', 'Middle Aged', 'Orthopedic Procedures', 'Reconstructive Surgical Procedures', 'Rupture', 'Tendons']
| 11,522,075
|
[['M01.060.057'], ['M01.060.116'], ['C26.558.554.213'], ['G01.154.090', 'G01.374.089'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C26.558.554'], ['M01.060.116.630'], ['E02.718', 'E04.555'], ['E04.680'], ['C26.761'], ['A02.880']]
|
['Named Groups [M]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Risperidone and haloperidol promote survival of stem cells in the rat hippocampus.
|
Altered neuroplasticity contributes to the pathophysiology of schizophrenia. However, the idea that antipsychotics may act, at least in part, by normalizing neurogenesis has not been consistently supported. Our study seeks to determine whether hippocampal cell proliferation is altered in adult rats pretreated with ketamine, a validated model of schizophrenia, and whether chronic administration with neuroleptic drugs (haloperidol and risperidone) affect changes of cell genesis/survival. Ketamine per se has no effect on cell proliferation. Its withdrawal, however, significantly induced cell proliferation/survival in the hippocampus. Risperidone and haloperidol supported cell genesis/survival as well. During ketamine withdrawal, however, their application did not affect cell proliferation/survival additionally. TUNEL staining indicated a cell-protective potency of both neuroleptics with respect to a ketamine-induced cell death. As RT-PCR and Western blot revealed that the treatment effects of risperidone and haloperidol seemed to be mediated through activation of VEGF and MMP2. The mRNA expression of NGF, BDNF, and NT3 was unaffected. From the respective receptors, only TrkA was enhanced when ketamine withdrawal was combined with risperidone or haloperidol. Risperidone also induced BCL-2. Ketamine withdrawal has no effect on the expression of VEGF, MMP2, or BCL-2. It activated the expression of BDNF. This effect was normalized by risperidone or haloperidol. The findings indicate a promoting effect of risperidone and haloperidol on survival of young neurons in the hippocampus by enhancing the expression of the anti-apoptotic protein BCL-2 and by activation of VEGF/MMP2, whereby an interference with ketamine and thus a priority role of the NMDA system was not evident.
|
['Adult Stem Cells', 'Animals', 'Antipsychotic Agents', 'Bromodeoxyuridine', 'Cell Proliferation', 'Cell Survival', 'Gene Expression Regulation', 'Haloperidol', 'Hippocampus', 'In Situ Nick-End Labeling', 'Male', 'Models, Animal', 'Nerve Tissue Proteins', 'RNA, Messenger', 'Rats', 'Rats, Sprague-Dawley', 'Risperidone', 'Time Factors']
| 19,579,000
|
[['A11.872.040'], ['B01.050'], ['D27.505.696.277.950.040', 'D27.505.954.427.210.950.040', 'D27.505.954.427.700.872.331'], ['D03.383.742.680.852.300.150', 'D13.570.230.430.196', 'D13.570.685.852.300.150'], ['G04.161.750', 'G07.345.249.410.750'], ['G04.346'], ['G05.308'], ['D02.522.352.506'], ['A08.186.211.180.405', 'A08.186.211.200.885.287.500.345'], ['E05.393.475'], ['E05.598'], ['D12.776.631'], ['D13.444.735.544'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.750'], ['D03.383.742.698.685'], ['G01.910.857']]
|
['Anatomy [A]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Does familiarity affect the enjoyment of touchscreen games for people with dementia?
|
INTRODUCTION: Previous research has indicated that people living with dementia are able to use touchscreen technology, which presents an opportunity to deliver meaningful and engaging activities for people to pass the time independently. The challenge is to identify suitable applications from the thousands that are currently available, and familiarity, where an app is a digital version of an existing real world game, may be one solution.OBJECTIVES: To evaluate the concept of familiarity in gameplay with people living with dementia by comparing a known game with a novel game and measuring whether users are able to play these games independently and whether they enjoy doing so.METHODS: Thirty older adults living with dementia were recruited from local care services. Each participant was assigned to one of two groups. Group 1 played a familiar game (Solitaire) and Group 2 played a novel game (Bubble Xplode). Each participant played the same game on three separate occasions within one week. Number of gameplay attempts, whether a checkpoint was reached and how much time to reach the checkpoint were measured. A brief post-session interview was conducted to assess the participants' enjoyment.RESULTS: Ninety percent of participants attempted gameplay independently with 17% of participants in the familiar group reaching the checkpoint compared with 93% playing the novel game. Regardless of which game was played or whether the checkpoint was reached, 88% of all participants reported enjoyment of the gaming sessions.DISCUSSION: People living with dementia can play touchscreen games independently, but familiarity does not ensure successful gameplay. Enjoyment appears to be independent of progression through a game. The potential of novel and unfamiliar games as meaningful activities that people with dementia can engage with independently should be further explored.
|
['Aged', 'Aged, 80 and over', 'Dementia', 'Female', 'Humans', 'Male', 'Memory', 'Pleasure', 'Recognition, Psychology', 'Touch', 'User-Computer Interface', 'Video Games']
| 26,897,552
|
[['M01.060.116.100'], ['M01.060.116.100.080'], ['C10.228.140.380', 'F03.615.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F02.463.425.540'], ['F01.470.867', 'F02.830.816.492'], ['F02.463.425.540.706'], ['F02.830.816.850', 'G11.561.790.850'], ['L01.224.900.910'], ['I03.450.642.693.930', 'L01.224.900.930']]
|
['Named Groups [M]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Anthropology, Education, Sociology, and Social Phenomena [I]']
| 0
| 1
| 1
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 1
| 0
| 0
|
Modeling early stage atherosclerosis in a primary human vascular microphysiological system.
|
Novel atherosclerosis models are needed to guide clinical therapy. Here, we report an in vitro model of early atherosclerosis by fabricating and perfusing multi-layer arteriole-scale human tissue-engineered blood vessels (TEBVs) by plastic compression. TEBVs maintain mechanical strength, vasoactivity, and nitric oxide (NO) production for at least 4 weeks. Perfusion of TEBVs at a physiological shear stress with enzyme-modified low-density-lipoprotein (eLDL) with or without TNFá promotes monocyte accumulation, reduces vasoactivity, alters NO production, which leads to endothelial cell activation, monocyte accumulation, foam cell formation and expression of pro-inflammatory cytokines. Removing eLDL leads to recovery of vasoactivity, but not loss of foam cells or recovery of permeability, while pretreatment with lovastatin or the P2Y11 inhibitor NF157 reduces monocyte accumulation and blocks foam cell formation. Perfusion with blood leads to increased monocyte adhesion. This atherosclerosis model can identify the role of drugs on specific vascular functions that cannot be assessed in vivo.
|
['Arterioles', 'Atherosclerosis', 'Biomechanical Phenomena', 'Cell Adhesion', 'Cell Proliferation', 'Cells, Cultured', 'Foam Cells', 'Humans', 'Lipoproteins, LDL', 'Models, Biological', 'Monocytes', 'Nitric Oxide', 'Tissue Engineering', 'Tumor Necrosis Factor-alpha']
| 33,110,060
|
[['A07.015.114.060', 'A07.015.461.080'], ['C14.907.137.126.307'], ['G01.154.090', 'G01.374.089'], ['G04.022'], ['G04.161.750', 'G07.345.249.410.750'], ['A11.251'], ['A11.329.372.368', 'A11.627.482.368', 'A11.733.397.368', 'A15.382.670.522.368', 'A15.382.680.397.368'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D10.532.515', 'D12.776.521.550'], ['E05.599.395'], ['A11.118.637.555.652', 'A11.148.580', 'A11.627.624', 'A11.733.547', 'A15.145.229.637.555.652', 'A15.378.316.580', 'A15.382.490.555.652', 'A15.382.670.547', 'A15.382.680.547'], ['D01.339.387', 'D01.625.550.500', 'D01.625.700.500', 'D01.650.550.587.600'], ['E05.481.500.311.500', 'J01.293.069.249.500'], ['D12.644.276.374.500.800', 'D12.644.276.374.750.626', 'D12.776.124.900', 'D12.776.395.930', 'D12.776.467.374.500.800', 'D12.776.467.374.750.626', 'D23.529.374.500.800', 'D23.529.374.750.626']]
|
['Anatomy [A]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Technology, Industry, and Agriculture [J]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Effects of Long-Term Denosumab on Bone Histomorphometry and Mineralization in Women With Postmenopausal Osteoporosis.
|
Context: Denosumab is a potent antiresorptive agent that reduces fractures in postmenopausal women with osteoporosis.Objective: Determine effects of up to 10 years of denosumab on bone histology, remodeling, and matrix mineralization characteristics.Design and Setting: International, multicenter, randomized, double-blind trial [Fracture Reduction Evaluation of Denosumab in Osteoporosis Every 6 Months (FREEDOM)] with a long-term open-label extension.Patients: Postmenopausal women with osteoporosis (92 women in FREEDOM, 46 in extension) who provided iliac bone biopsies, including 11 who provided biopsies at multiple time points.Interventions: FREEDOM subjects were randomized 1:1 to subcutaneous denosumab 60 mg or placebo every 6 months for 3 years. Long-term extension subjects continued receiving denosumab, open-label, for 7 additional years.Outcomes: Bone histology, histomorphometry, matrix mineralization.Results: Ten-year denosumab biopsies showed normal histology. Bone histomorphometry indicated normal bone structure and reduced bone remodeling after 10 years of denosumab, similar to levels after 2 and/or 3 and 5 years of denosumab. The degree of mineralization of bone was increased and mineralization heterogeneity was reduced in the denosumab years 2/3 group vs placebo. Changes in these mineralization variables progressed from years 2/3 to year 5 of denosumab, but not thereafter.Conclusions: Denosumab for 2/3, 5, and 10 years was associated with normal histology, low bone remodeling rate, increased matrix mineralization, and lower mineralization heterogeneity compared with placebo. These variables were unchanged from year 5 to year 10. These data, in combination with the maintenance of low fracture rates for up to 10 years as previously reported with denosumab therapy, suggest that strong, prolonged remodeling inhibition does not impair bone strength.
|
['Aged', 'Bone Density Conservation Agents', 'Bone Remodeling', 'Calcification, Physiologic', 'Denosumab', 'Double-Blind Method', 'Drug Administration Schedule', 'Female', 'Humans', 'Middle Aged', 'Osteoporosis, Postmenopausal', 'Time Factors', 'Treatment Outcome']
| 29,672,714
|
[['M01.060.116.100'], ['D27.505.696.242'], ['G11.427.213', 'G16.762.150'], ['G07.345.155.500', 'G07.345.500.325.377.625.050.500.175', 'G11.427.578.050.500.175'], ['D12.776.124.486.485.114.224.060.782', 'D12.776.124.790.651.114.224.060.782', 'D12.776.377.715.548.114.224.200.782'], ['E05.318.370.300', 'E05.581.500.300', 'N05.715.360.325.320', 'N06.850.520.445.300'], ['E02.319.283'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['C05.116.198.579.610', 'C18.452.104.579.610'], ['G01.910.857'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Illicit methadone injecting during methadone maintenance treatment in a specialised out-patient clinic.
|
AIM: The injection of non-sterile methadone designed for oral consumption is associated with serious health risks. There is only a small number of studies on this topic, with divergent results. The main aim of the present study was to obtain data on the frequency of methadone injecting in a state out-patient clinic specialised in substance use disorders.METHODS: Eighty patients in methadone maintenance treatment were interviewed with a short questionnaire. Mean age was 32 years, 76% were male, mean methadone dose was 55 mg.RESULTS: Twenty-six patients (32%) indicated having injected methadone at least once in their life. Only four patients (5%) reported having injected methadone within the preceding month (mean dose 56 mg). All four had injected additional substances during this month.CONCLUSIONS: Frequency rates appeared low compared with other studies despite a generous take-away policy. The results suggest an association between methadone injecting and a more general tendency to inject substances.
|
['Adult', 'Ambulatory Care Facilities', 'Cross-Sectional Studies', 'Female', 'Humans', 'Injections, Intravenous', 'Male', 'Methadone', 'Substance-Related Disorders', 'Surveys and Questionnaires', 'Switzerland']
| 16,380,851
|
[['M01.060.116'], ['N02.278.035'], ['E05.318.372.500.875', 'N05.715.360.330.500.875', 'N06.850.520.450.500.875'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.319.267.082.750', 'E02.319.267.530.540'], ['D02.522.675'], ['C25.775', 'F03.900'], ['E05.318.308.980', 'N05.715.360.300.800', 'N06.850.520.308.980'], ['Z01.542.883']]
|
['Named Groups [M]', 'Health Care [N]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Geographicals [Z]']
| 0
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
[Influence of blood pressure control on maintenance of residual function in patients treated by haemodialysis].
|
INTRODUCTION: Residual renal function (RRF) in the patients treated by haemodialysis (HD) is associated not only with better volume and blood pressure control but also with better metabolic control.The condition of the cardiovascular system significantly affects RRF.OBJECTIVE: The aim of the study was to find if there was any association between blood pressure regulation and the achieved HD ultrafiltration in the first year of haemodialysis treatment and the maintenance of RRF.METHODS: In this retrospective study, 53 patients were analyzed in the period 1994-2002. Residual clearance of urea (RCU) was measured for the first time at the beginning of HD treatment, and for the second time one year later. Laboratory data and values of blood pressure as well as the achieved HD ultrafiltration were taken from the electronic database of the Nephrology Hospital.RESULTS: The value of RCU less than 1 ml/min was considered as the loss of RRF and, at the beginning of HD treatment, 14 patients (26.4%) had that result. The rise of mean arterial pressure (MAP) was associated in linear regression analysis with a drop of residual diuresis volume (beta = -0.28; p = 0.04), but there was no association with RCU. The patients with MAP > 105 mm Hg had RKU less than the patients with MAP < 105 mm Hg (t = 2.23; p = 0.03). The rise of the HD ultrafiiltration significantly affected the loss of RRF obtained by the linear regression analysis (beta = -0.44; p = 0.0001).CONCLUSION: The greater HD ultrafiltration is related to a drop of RCU values. Only prospective randomised trials with the use of multiple regression analysis could define a more precise association between hypertension and RKU.
|
['Blood Pressure', 'Female', 'Humans', 'Kidney', 'Kidney Failure, Chronic', 'Male', 'Middle Aged', 'Renal Dialysis', 'Urea']
| 19,459,562
|
[['E01.370.600.875.249', 'G09.330.380.076'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A05.810.453'], ['C12.777.419.780.750.500', 'C13.351.968.419.780.750.500'], ['M01.060.116.630'], ['E02.870.300', 'E02.912.800'], ['D02.065.950']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Anatomy [A]', 'Diseases [C]', 'Named Groups [M]', 'Chemicals and Drugs [D]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Cancer. A potentially preventable disease.
|
Current epidemiologic and carcinogenesis data are lending credence to the hypothesis that individual cancers have specific causes. Because of this, most cancers are at least potentially preventable. Primary prevention, with removal of specific etiological agents, is the best means of prevention, such as elimination of tobacco smoke. Short of knowing the specific etiology, new chemo-prevention techniques are now being studied evaluating hormonal agents, anti-inflammatory drugs, and the structural analogs of vitamin A.
|
['Aspirin', 'Environmental Exposure', 'Female', 'Finasteride', 'Humans', 'Isotretinoin', 'Life Style', 'Male', 'Middle Aged', 'Neoplasms', 'Risk Factors', 'Tamoxifen']
| 7,775,840
|
[['D02.455.426.559.389.657.410.595.176'], ['N06.850.460.350'], ['D04.210.500.054.079.500', 'D04.210.500.925.100.250'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D02.455.326.271.665.202.495.325', 'D02.455.426.392.368.367.379.249.700.325', 'D02.455.849.131.495.325', 'D23.767.261.700.325'], ['F01.829.458'], ['M01.060.116.630'], ['C04'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['D02.455.426.559.389.150.700.900']]
|
['Chemicals and Drugs [D]', 'Health Care [N]', 'Organisms [B]', 'Psychiatry and Psychology [F]', 'Named Groups [M]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
BrainLab VectorVision Neuronavigation System: technology and clinical experiences in 131 cases.
|
OBJECTIVE: The BrainLab VectorVision neuronavigation system was used in 131 cases of different brain pathological conditions. The neuronavigation system was used without problems in 125 cases. These cases included 114 microsurgical operations, 4 endoscopic procedures, 4 frameless stereotactic biopsies, and 3 catheter placements.METHODS: The BrainLab VectorVision neuronavigation system is an intraoperative, image-guided, frameless, localization system. The system consists of a computer workstation for registration of images and physical spaces, an intraoperative localization device, and a computer image display. The system provides real-time responses regarding the locations of surgical instruments. VectorVision is based on passive reflections of infrared flashes. Universal adapters with reflective markers for surgical instruments, endoscopes, and the operating microscope are used.RESULTS: In six cases, the system could not be used because of system failure or mishandling. In 125 neurosurgical cases, the neuronavigation system was useful, with a target-localizing accuracy of 4+/-1.4 mm (mean+/-standard deviation). For small cerebral lesions, we never performed an exploration with negative results.CONCLUSION: The BrainLab neuronavigation system has been shown to be very helpful and user-friendly for routine neurosurgical interventions. Its advantage lies in its mobility, based on wireless reflective adapters for surgical instruments, endoscopes, and the operating microscope.
|
['Biopsy', 'Brain', 'Brain Diseases', 'Brain Neoplasms', 'Computer Systems', 'Endoscopes', 'Equipment Failure Analysis', 'Humans', 'Image Processing, Computer-Assisted', 'Microsurgery', 'Robotics', 'Stereotaxic Techniques', 'Surgical Equipment', 'Surgical Instruments', 'Video Recording']
| 9,894,969
|
[['E01.370.225.500.384.100', 'E01.370.225.998.054', 'E01.370.388.100', 'E04.074', 'E05.200.500.384.100', 'E05.200.998.054', 'E05.242.384.100'], ['A08.186.211'], ['C10.228.140'], ['C04.588.614.250.195', 'C10.228.140.211', 'C10.551.240.250'], ['L01.224.230'], ['E07.230.220', 'E07.858.240'], ['E05.325.192'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['L01.224.308'], ['E04.494', 'E05.591.580'], ['H01.671.293.643', 'J01.897.104.834', 'L01.224.050.375.630'], ['E04.525.800', 'E05.873'], ['E07.858'], ['E07.858.700'], ['L01.280.960']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Diseases [C]', 'Information Science [L]', 'Organisms [B]', 'Disciplines and Occupations [H]', 'Technology, Industry, and Agriculture [J]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 0
|
Pharmacodynamics of T cell function for monitoring pharmacologic immunosuppression after allogeneic hematopoietic stem cell transplantation.
|
Information on pharmacodynamic monitoring after allogeneic hematopoietic cell transplantation (allo-SCT) to evaluate individual responses to immunosuppressive drugs is scarce. We studied the relationship between a panel of pharmacodynamic markers monitored during the first 3 months after transplant and the occurrence of graft-versus-host disease (GVHD). Lymphocyte activation assessed by intracellular ATP concentration in CD4+ T cells, a high percentage of CD8+ effector T cells, and a low percentage of CD4+ regulatory T (Treg) cells correlated significantly with GVHD. A cutoff value of 0.5 for the CD8+ effector T/Treg ratio provided the most accurate diagnosis of GVHD (sensitivity 58.8%, specificity 91%). These pharmacodynamic markers may provide an efficient complement to standard pharmacokinetic monitoring of immunosuppressive drugs after allo-SCT.
|
['Adult', 'Aged', 'Allografts', 'Biomarkers', 'CD4-CD8 Ratio', 'Drug Monitoring', 'Female', 'Graft vs Host Disease', 'Hematopoietic Stem Cell Transplantation', 'Humans', 'Immunosuppression', 'Immunosuppressive Agents', 'Lymphocyte Activation', 'Male', 'Middle Aged', 'Pilot Projects', 'T-Lymphocytes']
| 27,882,485
|
[['M01.060.116'], ['M01.060.116.100'], ['A01.941.500'], ['D23.101'], ['E01.370.225.500.195.107.595.500.150.160', 'E01.370.225.625.107.595.500.150.160', 'E05.200.500.195.107.595.500.150.160', 'E05.200.625.107.595.500.150.160', 'E05.242.195.107.595.500.150.160', 'G04.140.107.595.500.150.160', 'G09.188.105.595.500.150.160', 'G12.248'], ['E01.370.520.200'], ['C20.452'], ['E02.095.147.500.500.500', 'E04.936.225.687.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.095.465.425.450', 'E05.478.610'], ['D27.505.696.477.656'], ['E01.370.225.812.482', 'E05.200.812.482', 'E05.478.594.530', 'G12.450.050.400.545', 'G12.565'], ['M01.060.116.630'], ['E05.318.372.750', 'E05.337.737', 'N05.715.360.330.720', 'N06.850.520.450.720'], ['A11.118.637.555.567.569', 'A15.145.229.637.555.567.569', 'A15.382.490.555.567.569']]
|
['Named Groups [M]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Organisms [B]', 'Health Care [N]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
[Focusing and targeting gallstones during extracorporeal shock waves lithotripsy].
|
The gallbladder lies at the junction of the medial segment of the left and right hepatic lobes. Besides the anatomic location, other factors may interfere with an ideal targeting of gallstones during extracorporeal shock waves treatment. Obesity, malnourishment, the respiratory cycle, the presence of intestinal gas, and colonic content may interfere with an adequate focalization. The use of a multipurpose device able to couple with the anterior abdominal wall like the Lithostar Plus permits to perform the procedure with the patient in supine, oblique or intercostal position. Varying the position permits to avoid interferences due to intestinal gas or intestinal content. The movements of the patients which produce a missing image can be easily refocussed. Technical ability, experience and a good machine permits a successful rate of gallstone fragmentation.
|
['Cholelithiasis', 'Gallbladder', 'Gallstones', 'Gases', 'Gastrointestinal Contents', 'Humans', 'Intestines', 'Lithotripsy', 'Obesity', 'Posture', 'Ultrasonography']
| 19,256,141
|
[['C06.130.409'], ['A03.159.439'], ['C06.130.409.633', 'C06.130.564.332.500', 'C23.300.175.525'], ['D01.362'], ['A12.519'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A03.556.124'], ['E02.600', 'E04.943.500'], ['C18.654.726.500', 'C23.888.144.699.500', 'E01.370.600.115.100.160.120.699.500', 'G07.100.100.160.120.699.500'], ['G11.427.695'], ['E01.370.350.850']]
|
['Diseases [C]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Leg amputation accelerates senescence of rat lumbar intervertebral discs.
|
STUDY DESIGN: Several senescence biomarkers were observed to investigate cell senescence in degenerative intervertebral lumbar discs of foreleg-amputated rats.OBJECTIVE: To determine if cell senescence is accelerated in degenerative intervertebral lumbar disc cells in an upright-rat model.SUMMARY OF BACKGROUND DATA: Cellular senescence was accelerated in human and sand rat degenerative intervertebral disc (IVD) cells. Repeated use of upright posture by rats contributed to degenerative disc changes. No convincing evidence of cell senescence was observed in the lumbar disc of the foreleg amputated rat.METHODS: The forelimbs of 20 rats were amputated at 1 month of age such that they maintained an upright stance; rats were housed in custom-made cages. Nonamputated rats, also 1 month of age, were kept in regular cages and served as a control group. The lumbar IVDs were harvested from rats in 2 groups, at 5 or 9 months following amputation. Senescence-associated-â-galactosidase-positive staining was used to detect cell senescence. p16INK4a and p27KIP were assessed by immunohistochemistry analysis. Total RNA isolated from these samples was used to measure the gene expression of p16INK4a, RB, cyclin D1, CDK4, PTEN, p27KIP, p19ARF, p21, TERT, and RAGE by real-time polymerase chain reaction assay.RESULTS: The highest levels of SA-â-GAL activity were detected in 9-month amputated rats. Quantitative immunohistochemical analysis showed that there were highest rates of p16INK4a and p27KIP protein expression in the cartilage endplate and anulus fibrosus of 9-month amputated rats. The mRNA levels of p16INK4a, RB, PTEN, p27KIP, p19ARF, and RAGE were upregulated. The increased cyclin D1 mRNA level was statistically significant only at the ninth month following amputation; CDK4 and TERT mRNA levels were downregulated to a similar extent at both points compared with nonamputated controls. mRNA expression of p21 was significantly downregulated.CONCLUSION: Accelerated cell senescence was associated with forelimb amputation that causes abnormal loading in rat lumbar IVDs.
|
['Amputation', 'Analysis of Variance', 'Animals', 'Biomarkers', 'Cellular Senescence', 'Cyclin-Dependent Kinase Inhibitor p27', 'Forelimb', 'Gene Expression', 'Genes, p16', 'Immunohistochemistry', 'Intervertebral Disc', 'Lumbar Vertebrae', 'Male', 'RNA, Messenger', 'Random Allocation', 'Rats', 'Rats, Sprague-Dawley', 'Reverse Transcriptase Polymerase Chain Reaction', 'beta-Galactosidase']
| 20,938,386
|
[['E04.555.080'], ['E05.318.740.150', 'N05.715.360.750.125', 'N06.850.520.830.150'], ['B01.050'], ['D23.101'], ['G04.043'], ['D12.644.360.225.600', 'D12.776.167.187.600', 'D12.776.476.225.600', 'D12.776.624.776.355.600'], ['A13.395'], ['G05.297'], ['G05.360.340.024.340.375.249.375', 'G05.360.340.024.340.415.400.375'], ['E01.370.225.500.607.512', 'E01.370.225.750.551.512', 'E05.200.500.607.512', 'E05.200.750.551.512', 'E05.478.583', 'H01.158.100.656.234.512', 'H01.158.201.344.512', 'H01.158.201.486.512', 'H01.181.122.573.512', 'H01.181.122.605.512'], ['A02.165.308.410', 'A02.835.232.834.432', 'A10.165.382.350.050'], ['A02.835.232.834.519'], ['D13.444.735.544'], ['E05.318.370.700', 'E05.581.500.805', 'N05.715.360.325.675', 'N06.850.520.445.700'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.750'], ['E05.393.620.500.725'], ['D08.811.277.450.410.100']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Disciplines and Occupations [H]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
|
13
|
The Y145Stop prion protein (PrP23-144), which has been linked to the development of a heritable prionopathy in humans, is a valuable in vitro model for elucidating the structural and molecular basis of amyloid seeding specificities. Here we report the sequential backbone and side-chain 13C and 15N assignments of mouse and Syrian hamster PrP23-144 amyloid fibrils determined by using 2D and 3D magic-angle spinning solid-state NMR. The assigned chemical shifts were used to predict the secondary structures for the core regions of the mouse and Syrian hamster PrP23-144 amyloids, and the results compared to those for human PrP23-144 amyloid, which has previously been analyzed by solid-state NMR techniques.
|
['Amino Acid Sequence', 'Amyloid', 'Animals', 'Cricetinae', 'Mice', 'Nuclear Magnetic Resonance, Biomolecular', 'Peptide Fragments', 'Prion Proteins', 'Protein Multimerization', 'Protein Structure, Secondary']
| 28,004,358
|
[['G02.111.570.060', 'L01.453.245.667.060'], ['D05.500.049', 'D12.776.049'], ['B01.050'], ['B01.050.150.900.649.313.992.635.075.250'], ['B01.050.150.900.649.313.992.635.505.500'], ['E05.196.867.519.550'], ['D12.644.541'], ['D12.776.395.550.448.600', 'D12.776.543.484.500.625', 'D12.776.543.550.418.600', 'D12.776.785.340'], ['G02.111.694'], ['G02.111.570.820.709.600']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Production of interleukin-12 is under the control of endogenous interleukin-10 in myocardial ischemia-reperfusion.
|
Interleukin (IL)-12 is a heterodimeric cytokine that is secreted mainly by antigen-presenting cells and plays a key role in determining the nature of immune response to exogenous or endogenous antigens. Negative regulators of IL-12 production include IL-10. With use of wild-type and IL-10-deficient mice, the aim of the current investigation was to determine whether IL-12 is produced in myocardial reperfusion injury and whether endogenous IL-10 modulates its production. IL-10 levels were significantly higher than baseline at both 2 h and 6 h after the start of the reperfusion. In the IL-10-deficient animals, no IL-12 could be detected in the plasma. In the wild-type animals, at baseline, and at 1-6 h after myocardial ischemia-reperfusion, no detectable increases in IL-12 were measured. However, in the IL-10-deficient mice, a significant and pronounced increase in IL-12 was detected. IL-10-deficient mice also exhibited significantly higher mortality during reperfusion than wild-type animals. We conclude that the production of IL-12 in myocardial reperfusion injury is dramatically affected by the levels of endogenous IL-10.
|
['Animals', 'Interleukin-10', 'Interleukin-12', 'Male', 'Mice', 'Mice, Inbred C57BL', 'Mice, Knockout', 'Myocardial Ischemia', 'Myocardial Reperfusion Injury']
| 11,198,362
|
[['B01.050'], ['D12.644.276.374.465.510', 'D12.776.467.374.465.510', 'D23.529.374.465.510'], ['D12.644.276.374.465.512', 'D12.776.467.374.465.512', 'D23.529.374.465.512'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.420', 'B01.050.150.900.649.313.992.635.505.500.400.420'], ['B01.050.050.136.500.500', 'B01.050.150.900.649.313.992.635.505.500.550.455', 'B01.050.150.900.649.313.992.635.505.500.800.500'], ['C14.280.647', 'C14.907.585'], ['C14.280.238.615', 'C14.280.647.625', 'C14.907.585.625', 'C14.907.725.600', 'C23.550.767.877.500']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
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