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Embryonic stem cells improve skeletal muscle recovery after extreme atrophy in mice.
|
INTRODUCTION: We injected embryonic stem cells into mouse tibialis anterior muscles subjected to botulinum toxin injections as a model for reversible neurogenic atrophy.METHODS: Muscles were exposed to botulinum toxin for 4 weeks and allowed to recover for up to 6 weeks. At the onset of recovery, a single muscle injection of embryonic stem cells was administered. The myofiber cross-sectional area, single twitch force, peak tetanic force, time-to-peak force, and half-relaxation time were determined.RESULTS: Although the stem cell injection did not affect the myofiber cross-sectional area gain in recovering muscles, most functional parameters improved significantly compared with those of recovering muscles that did not receive the stem cell injection.CONCLUSIONS: Muscle function recovery was accelerated by embryonic stem cell delivery in this durable neurogenic atrophy model. We conclude that stem cells should be considered a potential therapeutic tool for recovery after extreme skeletal muscle atrophy.
|
['Animals', 'Botulinum Toxins', 'Embryonic Stem Cells', 'Humans', 'Male', 'Mice', 'Muscle, Skeletal', 'Muscular Atrophy', 'Recovery of Function', 'Stem Cell Transplantation']
| 24,934,406
|
[['B01.050'], ['D08.811.277.656.300.480.153', 'D08.811.277.656.675.374.153', 'D12.776.097.156', 'D23.946.123.179'], ['A11.872.700.250'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['B01.050.150.900.649.313.992.635.505.500'], ['A02.633.567', 'A10.690.552.500'], ['C10.597.613.612', 'C23.300.070.500', 'C23.888.592.608.612'], ['G16.757'], ['E02.095.147.500.500', 'E04.936.225.687']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
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[Experimental study on adenosine triphosphate combining bone marrow mesenchymal stem cells transplantation in treatment of spinal cord injury in rats].
|
OBJECTIVE: Adenosine triphosphate (ATP) can promote the repair of spinal cord injury (SCI). To investigate the effect of ATP combined with bone marrow mesenchymal stem cells (BMSCs) transplantation on SCI, and to evaluate the synergistic action of ATP and BMSCs in the repair of SCI and the feasibility of the combined transplantation in the treatment of SCI.METHODS: BMSCs were isolated from the marrow of the tibia and the femur of a male SD rat (weighing 120 g), the 3rd generation BMSCs were labeled with BrdU, then BMSCs suspension of 5.0 x 10(7) cell/mL were prepared. Forty-eight adult female SD rats (weighing 240-260 g) were made SCI models at T2 levels according to the improved Allen's method, and were randomly divided into 4 groups (groups A, B, C, and D, n = 12). In group A, ATP (40 mg/kg) and BMSCs (6 microL) were injected to the central point and the other 2 points which were 1 mm from the each side of head and tail of the injured spinal cord; after blending the BMSCs suspension, the cells amount was about 3.0 x 10(5). In groups B, C, and D, the BMSCs suspension (6 microL), ATP (40 mg/kg), and PBS (40 mg/kg) were injected to the points by the same method as group A, respectively. The general conditions of the rats were observed after operation. The nerve function of low extremities was evaluated using the improved Tarlov scale and the Rivlin inclined plane test at 1, 3, 7, 14, 21, and 28 days after operation. At 28 days after operation, the reparative effect of SCI was observed using histological and immunohistochemical staining.RESULTS: One rat of group A, 2 of group B, 2 of group C, and 3 of group D died of infection and anorexic, the others survived to the end of the experiment. Paralysis symptom in low extremities occurred in all rats after operation and was improved at 2-3 weeks postoperatively, the improvement of group A was the best, groups B and C were better, group D was the worst. There was no significant difference in the Tarlov scale and the Rivlin inclined plane test among 4 groups at 1 and 3 days after operation and between groups B and C at 7, 14, 21, and 28 days after operation (P > 0.05), but there were significant differences among other groups at 7, 14, 21, and 28 days after operation (P < 0.05). At 28 days after operation, HE staining demonstrated that the injured region in group A was finely restored, without obvious scar tissue and cavity, and there existed clear stem cell differentiation characters; there was small amount of scar tissue and cavity in the injury site of groups B and C; and there was great deal of scar tissue in the injury site of group D, in which there were numerous inflammatory cells and fibroblasts infiltration and bigger cavity. Immunohistochemical staining showed that BrdU-positive BMSCs were seen in groups A and B, and positive cells of group A was significantly more than that of group B (P < 0.05). The expressions of neurofilament protein 200 and glial fibrillary acidic protein in group A were significantly higher than those in groups B, C, and D, and groups B and C were significantly higher than group D (P < 0.05).CONCLUSION: ATP has protective effects on injured spinal cord, a combination of ATP and BMSCs can synergistically promote the reparation of SCI.
|
['Adenosine Triphosphate', 'Animals', 'Bone Marrow Cells', 'Cell Differentiation', 'Disease Models, Animal', 'Female', 'Male', 'Mesenchymal Stem Cell Transplantation', 'Rats', 'Rats, Sprague-Dawley', 'Spinal Cord Injuries']
| 21,046,813
|
[['D03.633.100.759.646.138.236', 'D13.695.667.138.236', 'D13.695.827.068.236'], ['B01.050'], ['A11.148', 'A15.378.316'], ['G04.152'], ['C22.232', 'E05.598.500', 'E05.599.395.080'], ['E02.095.147.500.500.625', 'E04.936.225.687.625'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.750'], ['C10.228.854.763', 'C10.900.850', 'C26.819']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
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Morphological transformation and DNA adduct formation by dibenz[a,h]anthracene and its metabolites in C3H10T1/2CL8 cells.
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The major routes of metabolic activation of dibenz[a,h]-anthracene (DBA) have been studied in transformable C3H10T1/2CL8 (C3H10T1/2) mouse embryo fibroblasts in culture. The morphological transforming activities of three potential intermediates formed by metabolism of DBA by C3H10T1/2 cells, trans-3,4-dihydroxy-3,4-dihydro-DBA-(DBA-3,4-diol), trans-dihydroxy-3,4-dihydro-DBA-anti-1,2-oxide (DBA-3,4-diol-1,2-oxide) and DBA-5,6-oxide were determined. DBA-3,4-diol-1,2-oxide was a strong morphological transforming agent giving a mean of 73% dishes with Type II or III foci and 1.63 Type II and III foci per dish at 0.5 microgram/ml. DBA-3,4-diol produced a mean of 42% dishes with Type II or III foci and 0.81 Type II and III foci per dish at 2.5 micrograms/ml. DBA gave a mean of 24% dishes with Type II or III foci and 0.29 Type II and III foci per dish at 2.5 micrograms/ml. DBA-5,6-oxide was found to be inactive. DNA adducts of DBA, DBA-3,4-diol, DBA-3,4-diol-1,2-oxide, DBA-1,4/2,3-tetrol and DBA-5,6-oxide in C3H10T1/2 cells were analyzed by 32P-postlabeling method. DBA gave 11 adducts, nine of which were observed in the DNA of cells treated with DBA-3,4-diol and seven from cells treated with DBA-3,4-diol-1,2-oxide. Two of these adducts that appear in each of the treatment groups have been identified as the product of the interaction of DBA-3,4-diol-1,2-oxide with 2'-deoxyguanosine. Furthermore, there is evidence for DBA-DNA adducts in cells treated with DBA, DBA-3,4-diol and DBA-3,4-diol-1,2-oxide arising from metabolism to (+,-)-trans,trans-3,4,10,11-tetrahydroxy-3,4,10,11-tetrahydro-DBA (DBA-3,4,10,11-bis-diol). These results are based on co-migration of C3H10T1/2 DNA adducts with skin DNA adducts formed after topical treatment of mice with DBA-3,4,10,11-bis-diol. In C3H10T1/2 cells, DBA is metabolically activated through DBA-3,4-diol, which is further activated via the DBA-3,4-diol-1,2-oxide and DBA-3,4,10,11-bis-diol pathways. No evidence is provided for the metabolism of DBA by the K-region pathway.
|
['Animals', 'Benz(a)Anthracenes', 'Biotransformation', 'Cell Transformation, Neoplastic', 'DNA Adducts', 'Fibroblasts', 'Isotope Labeling', 'Mice', 'Mice, Inbred C3H', 'Oxidation-Reduction', 'Phosphorus Radioisotopes', 'Structure-Activity Relationship']
| 7,955,058
|
[['B01.050'], ['D02.455.426.559.847.149', 'D04.615.149'], ['G03.171', 'G03.787.225', 'G07.690.725.225'], ['C04.697.098.500', 'C23.550.727.098.500'], ['D13.444.308.135', 'G05.200.104'], ['A11.329.228'], ['E05.522'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.388', 'B01.050.150.900.649.313.992.635.505.500.400.388'], ['G02.700', 'G03.295.531'], ['D01.268.666.500.604', 'D01.496.669.604', 'D01.496.749.658'], ['G02.111.830', 'G07.690.773.997']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Gene expression profiles in a porcine model of infarction: differential expression after intracoronary injection of heterologous bone marrow mesenchymal cells.
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Myocardial infarction is one of the main causes of mortality in developed countries. Injection of bone marrow mesenchymal stem cells (BMMSC) with the ability to regenerate lost cardiomyocytes is a promising therapy for heart failure. To evaluate this strategy, an in vivo porcine model of infarction was used. Gene expression profiles of 3 groups of pigs (n = 5 each) were analyzed and compared by real-time reverse transcription-polymerase chain reaction (RT-PCR). One of the groups underwent anterior descending coronary occlusion followed by BMMSC injection; a placebo group was injected with culture medium without cells after infarction; and a third group was formed by healthy pigs. Four weeks later, cells or medium was administered by intracoronary injection and, a month later, animals were sacrificed and samples collected. Genes related to cardiomyogenesis (Mef2C, Gata4, Nkx2.5), mobilization and homing of resident or circulating stem cells (Sdf1, Cxcr4, c-Kit), contractibility (Serca2a), and fibrosis (CollA1) were analyzed. Gene expression profiles changed in various heart areas in the 3 groups. Expression of genes related to cardiomyogenesis decreased in infarcted zones compared with homologous regions of healthy hearts. Sdf1 expression increased in the apex of infarcted hearts. Serca2a expression was reduced in the ventricles and atria of infarcted hearts. Also, increases in Cxcr4 and CollA1 expression were observed in infarcted hearts of cell-treated pigs compared with the placebo group. In conclusion, infarction induced changes in genes involved in various biological processes. Intracoronary injection of heterologous BMMSC resulted in localized changes in the expression of Cxcr4 and Col1A1.
|
['Animals', 'Collagen Type I', 'Disease Models, Animal', 'Gene Expression Profiling', 'Mesenchymal Stem Cell Transplantation', 'Myocardial Infarction', 'Swine']
| 19,715,896
|
[['B01.050'], ['D05.750.078.280.300.100', 'D12.776.860.300.250.300.100'], ['C22.232', 'E05.598.500', 'E05.599.395.080'], ['E05.393.332'], ['E02.095.147.500.500.625', 'E04.936.225.687.625'], ['C14.280.647.500', 'C14.907.585.500', 'C23.550.513.355.750', 'C23.550.717.489.750'], ['B01.050.150.900.649.313.500.880']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 1
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| 1
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Stigma never dies: Mourning a spouse who died of AIDS in China.
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Stigma towards people with HIV (PHIV) can affect their family members. In this study of 68 HIV seronegative participants in China whose spouse died of AIDS, 35.3% reported prolonged grief. Stigma beliefs towards PHIV (i.e., belief that PHIV's death leaves the deceased, the family and society better off) predicted grief symptoms. Social campaigns to combat stigma and grief therapy to reconstruct the meaning of HIV-related death may be helpful to reduce suffering in HIV bereaved.
|
['Acquired Immunodeficiency Syndrome', 'Adult', 'Bereavement', 'China', 'Cross-Sectional Studies', 'Death', 'Female', 'Humans', 'Male', 'Middle Aged', 'Social Stigma', 'Spouses', 'Suicide']
| 26,553,145
|
[['C01.221.250.875.040', 'C01.221.812.640.400.040', 'C01.778.640.400.040', 'C01.925.782.815.616.400.040', 'C01.925.813.400.040', 'C01.925.839.040', 'C20.673.480.040'], ['M01.060.116'], ['F01.470.142'], ['Z01.252.474.164'], ['E05.318.372.500.875', 'N05.715.360.330.500.875', 'N06.850.520.450.500.875'], ['C23.550.260'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['F01.145.813.840'], ['F01.829.263.500.660', 'I01.880.853.150.500.670', 'M01.816'], ['F01.145.126.980.875', 'I01.880.735.856']]
|
['Diseases [C]', 'Named Groups [M]', 'Psychiatry and Psychology [F]', 'Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Anthropology, Education, Sociology, and Social Phenomena [I]']
| 0
| 1
| 1
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| 1
| 1
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Design of switchable wettability sensor for paraquat based on clicking calix[4]arene.
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A calix[4]arene acetylene (C4AE)-modified gold surface is successfully constructed in situ via click chemistry. The functionalized surface is used for selective recognition of paraquat by a wettability switch. Impedance measurements showed that the surface also expresses recognition for paraquat with a high sensitivity of 10 pM. The recognition mode, based on host-guest inclusion, is studied by computational calculations and the possible mechanism is analyzed.
|
['Acetylene', 'Calixarenes', 'Click Chemistry', 'Electric Impedance', 'Electrochemical Techniques', 'Gold', 'Herbicides', 'Paraquat', 'Phenols', 'Sensitivity and Specificity', 'Surface Properties', 'Wettability']
| 22,407,032
|
[['D02.455.326.397.259'], ['D04.345.025'], ['E05.197.124', 'J01.897.836.249.124'], ['G01.358.500.249.277.350'], ['E05.301'], ['D01.268.556.322', 'D01.268.956.186', 'D01.552.544.322'], ['D27.720.031.700.366', 'D27.888.723.366'], ['D03.383.725.762.621'], ['D02.455.426.559.389.657'], ['E05.318.370.800', 'E05.318.740.872', 'G17.800', 'N05.715.360.325.700', 'N05.715.360.750.725', 'N06.850.520.445.800', 'N06.850.520.830.872'], ['G02.860'], ['G02.409.500', 'G02.860.908']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Technology, Industry, and Agriculture [J]', 'Phenomena and Processes [G]', 'Health Care [N]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
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Incidence, pathology and prevention of keel bone deformities in the laying hen.
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1. As a baseline study of the nature and incidence of keel deformities in laying hens, keel condition was examined in three different strains of hen from a total of 4 different caged environments (two commercial farms and two experimental farms). Incidence of keel deformity on farms in end of lay hens ranged from 2.6 to 16.7%. Only 0.8% of younger 15-week-old pullets had deformed keels. 2. Incidence of keel deformities was unchanged in 100 birds sampled from a free-range system compared to conventional caged siblings at the same farm. 3. Keel condition was also examined in 5 selected generations of a study involving the use of a body-weight-restricted selection index for skeletal improvement. Divergent selection for skeletal characteristics decreased incidence of keel deformity and improved radiographic density (RD) in high bone index (BI) hens compared to low BI hens in all selected generations. Male high BI keels were also improved compared to low BI. Shear strength measured in normal keels in generation 6 (G6) of the genetic study was improved in high BI hens compared to low BI hens. For all hens in the genetic study, those with normal keels had stronger tibiotarsus and humerus breaking strengths than hens with deformed keels. 4. Histopathology of keels representative of different deformities showed the presence of fracture callus material and new bone in all cases. This establishes that deformities are a result of trauma and are not developmental in origin. 5. Ash contents of keels, tibiae and humeri showed no differences between hens with normal and deformed keels. There were no differences in indicators of collagen cross-linkage in other bones between hens with normal keels and those with deformed keels. 6. It is concluded that lack of bone mass is the underlying cause of keel fracture and deformity in laying hens, rather than qualitative changes in bone, and that genetic selection can improve keel quality and prevent deformity.
|
['Animals', 'Biomechanical Phenomena', 'Bone Density', 'Bone and Bones', 'Chickens', 'Female', 'Fractures, Bone', 'Male', 'Minerals', 'Osteoporosis', 'Oviposition', 'Poultry Diseases', 'Selection, Genetic']
| 15,327,118
|
[['B01.050'], ['G01.154.090', 'G01.374.089'], ['G11.427.100'], ['A02.835.232', 'A10.165.265'], ['B01.050.150.900.248.350.150', 'B01.050.150.900.248.690.192'], ['C26.404'], ['D01.578'], ['C05.116.198.579', 'C18.452.104.579'], ['G08.686.784.480'], ['C22.131.728'], ['G05.783']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Diseases [C]', 'Chemicals and Drugs [D]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
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Anaerobic metabolism induces greater total energy expenditure during exercise with blood flow restriction.
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PURPOSE: We investigated the energy system contributions and total energy expenditure during low intensity endurance exercise associated with blood flow restriction (LIE-BFR) and without blood flow restriction (LIE).METHODS: Twelve males participated in a contra-balanced, cross-over design in which subjects completed a bout of low-intensity endurance exercise (30min cycling at 40% of [Formula: see text]) with or without BFR, separated by at least 72 hours of recovery. Blood lactate accumulation and oxygen uptake during and after exercise were used to estimate the anaerobic lactic metabolism, aerobic metabolism, and anaerobic alactic metabolism contributions, respectively.RESULTS: There were significant increases in the anaerobic lactic metabolism (P = 0.008), aerobic metabolism (P = 0.020), and total energy expenditure (P = 0.008) in the LIE-BFR. No significant differences between conditions for the anaerobic alactic metabolism were found (P = 0.582). Plasma lactate concentration was significantly higher in the LIE-BFR at 15min and peak post-exercise (all P?0.008). Heart rate was significantly higher in the LIE-BFR at 10, 15, 20, 25, and 30min during exercise, and 5, 10, and 15min after exercise (all P?0.03). Ventilation was significantly higher in the LIE-BFR at 10, 15, and 20min during exercise (all P?0.003).CONCLUSION: Low-intensity endurance exercise performed with blood flow restriction increases the anaerobic lactic and aerobic metabolisms, total energy expenditure, and cardiorespiratory responses.
|
['Adolescent', 'Adult', 'Anaerobiosis', 'Blood Circulation', 'Exercise', 'Female', 'Humans', 'Lactic Acid', 'Male', 'Oxygen Consumption', 'Young Adult']
| 29,596,452
|
[['M01.060.057'], ['M01.060.116'], ['G02.111.062', 'G03.078'], ['G09.330.100'], ['G11.427.410.698.277', 'I03.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D02.241.511.459.450'], ['G03.680'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Phenomena and Processes [G]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Organisms [B]', 'Chemicals and Drugs [D]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
|
[Foreign physicians practicing in France (problems posed by the application of the law of July 13, 1972)].
|
The 13 July 1972 law defined a procedure which applies to physicians wishing to practise in France but who don't meet the double requirement: having the French nationality (or being a citizen of a member state of the European Union) and holding the national diploma of Doctor of Medicine (or a diploma of the European Community). The Minister, in agreement with a commission in charge of examining the applications one by one, determines each year the number of authorizations to be possibly granted. The applications mainly come from physicians of the Mediterranean area who came to France to follow a specialized training and who, sometimes, have been practising in French hospitals for many years. Many of them have acquired french nationality. Since 1986, the Commission has been facing difficult problems due to a regularly increasing number of applications, meanwhile the number of authorizations, at least until 1991, has been going down on account of restricted intake. The "stock control" cannot be considers without parallel measures, especially for the recruitment of hospital practitioners. A deep legislative reshuffle should also be considered.
|
['Foreign Medical Graduates', 'France', 'Humans']
| 7,895,113
|
[['M01.526.407.435', 'M01.526.485.810.390', 'N02.360.810.390'], ['Z01.542.286'], ['B01.050.150.900.649.313.988.400.112.400.400']]
|
['Named Groups [M]', 'Health Care [N]', 'Geographicals [Z]', 'Organisms [B]']
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
The effect of age on the pharmacokinetics and pharmacodynamics of midazolam.
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OBJECTIVE: We investigated the pharmacologic properties of midazolam with special regard to age using the electroencephalogram (EEG) as a measure of the hypnotic-sedative effect.METHODS: Nine younger (24 to 28 years) and nine elderly (67 to 81 years) male volunteers received midazolam by a computer-controlled device. Two infusion cycles with linearly increasing target plasma levels (slope, 40 ng/mL/min for the younger subjects; 20 ng/mL/min for the elderly subjects) were administered until defined end points were attained (median EEG frequency <4 Hz and loss of responsiveness to acoustic stimuli). An EEG was recorded to quantitate the hypnotic effect, relating the median frequency of the power spectrum to the plasma level by a sigmoid Emax model, including an effect compartment. Pharmacokinetic data were derived from arterial blood samples with use of a three-compartment model.RESULTS: The total doses needed to reach the defined end points were 71+/-9 mg and 35+/-6 mg for the younger and elderly subjects, respectively (P < .001). Pharmacokinetic parameters were similar in both groups (clearance, 399+/-91 and 388+/-97 mL/min; steady-state volume of distribution, 85+/-22 and 104 +/-11 L in young and elderly subjects, respectively). Pharmacodynamic data showed a large difference in half-maximum concentration (EC50; young subjects, 522+/-236 ng/mL; elderly subjects, 223+/-56 ng/mL; P < .05), a steep concentration-response curve, and distinct hysteresis. We found much interindividual variability in the plasma concentrations necessary to achieve the clinical end points, regardless of age.CONCLUSIONS: These results suggest that the lower doses needed to reach sedation in the elderly subjects were attributable to a 50% decrease in EC50, not to changes in pharmacokinetics.
|
['Adult', 'Aged', 'Aged, 80 and over', 'Aging', 'Blood Pressure', 'Dose-Response Relationship, Drug', 'Electroencephalography', 'Female', 'Heart Rate', 'Humans', 'Hypnotics and Sedatives', 'Infusions, Intravenous', 'Male', 'Midazolam', 'Respiration', 'Volunteers']
| 10,391,668
|
[['M01.060.116'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['G07.345.124'], ['E01.370.600.875.249', 'G09.330.380.076'], ['G07.690.773.875', 'G07.690.936.500'], ['E01.370.376.300', 'E01.370.405.245'], ['E01.370.600.875.500', 'G09.330.380.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D27.505.696.277.350', 'D27.505.954.427.210.350'], ['E02.319.267.082.500', 'E02.319.267.510.590'], ['D03.633.100.079.080.575'], ['G09.772.705'], ['M01.955']]
|
['Named Groups [M]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Chemicals and Drugs [D]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Immune responses in normal Indian langur monkeys (Presbytis entellus)--a primate model for visceral leishmaniasis.
|
The Indian langur monkey (Presbytis entellus) is an experimental host for a range of human diseases and for the assessment of vaccine candidate antigens to some common parasitic infections. This experimental host is particularly suitable for the follow-up of immunological responses. To understand some of the mechanism that underlies the defense against experimental pathogens there is a need of the basic knowledge on antibody and cell mediated immune responses. In the present study 25 na?ve monkeys were subjected to for assessment of their antibody responses to various human parasitic antigens as well as mitogen induced cellular responses. Only few monkeys were found to have low titer of antiparasitic antibodies. There was compressive dose dependent proliferative response of peripheral blood mononuclear cells. Unlike humans, the blastogenic as well as cytokine responses (IFN-gamma, IL-2 and IL-4) to Con A was considerably higher as compared to PHA. These findings are similar to what have been reported in other non-human primates, confirming the appropriateness of Indian langurs for pre-clinical trials.
|
['Animals', 'Antibodies, Helminth', 'Antibodies, Monoclonal', 'Antibodies, Protozoan', 'Cercopithecidae', 'Cytokines', 'Disease Models, Animal', 'Enzyme-Linked Immunosorbent Assay', 'Immunity, Cellular', 'India', 'Leishmaniasis, Visceral', 'Leukocytes, Mononuclear', 'Male', 'Mitogens']
| 15,061,718
|
[['B01.050'], ['D12.776.124.486.485.114.185', 'D12.776.124.790.651.114.185', 'D12.776.377.715.548.114.185'], ['D12.776.124.486.485.114.224', 'D12.776.124.790.651.114.224', 'D12.776.377.715.548.114.224'], ['D12.776.124.486.485.114.252', 'D12.776.124.790.651.114.252', 'D12.776.377.715.548.114.252'], ['B01.050.150.900.649.313.988.400.112.199'], ['D12.644.276.374', 'D12.776.467.374', 'D23.529.374'], ['C22.232', 'E05.598.500', 'E05.599.395.080'], ['E05.478.566.350.170', 'E05.478.566.380.360', 'E05.478.583.400.170', 'E05.601.470.350.170', 'E05.601.470.380.360'], ['G12.450.050.400'], ['Z01.252.245.393'], ['C01.610.752.300.500.510', 'C01.920.813.510'], ['A11.118.637.555', 'A15.145.229.637.555', 'A15.382.490.555'], ['D27.505.519.593.624']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Geographicals [Z]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
|
Khellin, a naturally occurring furochromone, used for the photochemotherapy of skin diseases: mechanism of action.
|
Khellin, a naturally occurring furochromone, used in the past as a coronary vasodilator, has recently been used in the photochemotherapeutic treatment of vitiligo and psoriasis. With the aim of elucidating its mechanism of action, the interactions both in ground and excited states between the drug and DNA were studied in vitro. Khellin forms in the dark a molecular complex with DNA. By subsequent irradiation (365 nm) the drug photoconjugates covalently with the macromolecule, although the rate of photobinding is rather low. The in vivo photobinding of khellin to the DNA of Ehrlich ascites tumor cells is also low. In photoaddition with the macromolecule the drug forms inter-strand cross-links, although again in small amounts. The furan side monoadduct between khellin and thymine, formed in the photoreaction between the drug and DNA, was isolated and characterized, and shows a cis-syn configuration.
|
['Animals', 'Carcinoma, Ehrlich Tumor', 'Chemical Phenomena', 'Chemistry', 'Cross-Linking Reagents', 'DNA', 'Dialysis', 'Khellin', 'Methoxsalen', 'Photochemistry', 'Skin Diseases', 'Solubility', 'Spectrophotometry, Ultraviolet']
| 3,203,737
|
[['B01.050'], ['C04.557.470.200.200', 'C04.619.169'], ['G02'], ['H01.181'], ['D27.720.470.410.210'], ['D13.444.308'], ['E05.196.353', 'G02.186'], ['D03.383.663.283.446.794.300', 'D03.633.100.150.446.794.300', 'D03.633.300.770.300'], ['D03.383.663.283.446.794.500', 'D03.633.100.150.446.794.500', 'D03.633.300.770.500'], ['H01.181.529.711'], ['C17.800'], ['G02.805'], ['E05.196.712.726.802', 'E05.196.867.826.802']]
|
['Organisms [B]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Disciplines and Occupations [H]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
Prevalence of microsporidiosis due to Enterocytozoon bieneusi and Encephalitozoon (Septata) intestinalis among patients with AIDS-related diarrhea: determination by polymerase chain reaction to the microsporidian small-subunit rRNA gene.
|
Microsporidia are emerging as opportunistic pathogens in patients with AIDS. Enterocytozoon bieneusi and Encephalitozoon (Septata) intestinalis have been implicated in enteric infections in AIDS patients with chronic diarrhea, a wasting syndrome, and malabsorption. We used the polymerase chain reaction (PCR) and primers that amplify the conserved regions of the small-subunit rRNA (SSU-rRNA) gene of E. bieneusi and E. intestinalis in tissue specimens from HIV-infected patients with and without diarrhea to examine the association between microsporidia and diarrhea in patients with AIDS. Tissue specimens were obtained from 68 patients with AIDS and diarrhea (mean CD4 lymphocyte count, 21/mm3) and 43 AIDS patients without diarrhea (mean CD4 lymphocyte count, 60/mm3). By means of PCR with use of the SSU-rRNA primers specific for E. bieneusi and E. intestinalis, we found that 44% of patients with diarrhea were infected with microsporidia, whereas only 2.3% of the patients without diarrhea were infected with microsporidia (P < .001). There was a clear association between the presence of microsporidia and diarrhea. In addition, the SSU-rRNA primers proved to be sensitive and specific when used in this clinical setting.
|
['AIDS-Related Opportunistic Infections', 'Animals', 'Diarrhea', 'Encephalitozoon', 'Encephalitozoonosis', 'Humans', 'Microsporida', 'Microsporidiosis', 'Polymerase Chain Reaction', 'Prevalence', 'RNA, Protozoan', 'RNA, Ribosomal']
| 8,922,793
|
[['C01.221.250.875.100', 'C01.597.050', 'C01.610.684.050', 'C01.925.597.050', 'C01.925.782.815.616.400.100', 'C20.673.480.100'], ['B01.050'], ['C23.888.821.214'], ['B01.300.360.500.500.100.175'], ['C01.150.703.617.300'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['B01.300.360.500.500'], ['C01.150.703.617'], ['E05.393.620.500'], ['E05.318.308.985.525.750', 'N01.224.935.597.750', 'N06.850.505.400.975.525.750', 'N06.850.520.308.985.525.750'], ['D13.444.735.650'], ['D13.444.735.686']]
|
['Diseases [C]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Chemicals and Drugs [D]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Stages of delay in oral cancer care evaluated at a tertiary health centre.
|
BACKGROUND: To examine the stages of delay in presentation and management of oral cancer patients at University College Hospital Ibadan and compare findings with previous studies.METHODS: A 20-year retrospective analysis of the delay stages among oral cancer patients that utilized patient's biodata and clinical data.RESULT: 169 oral cancer cases consisting of 127 carcinomas, 25 sarcomas and 17 lymphomas were analyzed. There was significant difference in the mean evolution time (ET) according to histological type (oral carcinoma = 282.8 +/- 414, oral sarcomas = 219.2 +/- 247.3 and oral lymphomas 105.5 +/- 115 days; p = 0.001). Patient's delay was more than professional delay for all cancer types (65.9%, 59.1% and 60.1% for carcinomas, sarcomas and lymphomas respectively). There was a significant difference in the mean ET of the early stage cancers compared with the late stage cancers (mean = 137.2 +/- 99 and 266.4 +/- 355; p = 0.010).CONCLUSION: A combination of patients and professional delay negatively influenced the management of oral cancer patients but the patient's delay formed the bulk of this combination in our center.
|
['Adolescent', 'Adult', 'Aged', 'Aged, 80 and over', 'Analysis of Variance', 'Child', 'Female', 'Humans', 'Kaplan-Meier Estimate', 'Male', 'Middle Aged', 'Mouth Neoplasms', 'Nigeria', 'Retrospective Studies', 'Tertiary Care Centers', 'Time Factors', 'Time-to-Treatment', 'Young Adult']
| 24,839,739
|
[['M01.060.057'], ['M01.060.116'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['E05.318.740.150', 'N05.715.360.750.125', 'N06.850.520.830.150'], ['M01.060.406'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.740.998.650', 'N05.715.360.750.795.650', 'N06.850.520.830.998.650'], ['M01.060.116.630'], ['C04.588.443.591', 'C07.465.530'], ['Z01.058.290.190.565'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['N02.278.421.830'], ['G01.910.857'], ['E02.760.928', 'N02.421.585.928'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Diseases [C]', 'Geographicals [Z]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Increasing breast milk production for premature infants with a relaxation/imagery audiotape.
|
Many women whose premature infants are hospitalized in a newborn intensive care unit choose to express breast milk for their babies. Yet anxiety, fatigue, and emotional stress are powerful inhibitors of lactation. To facilitate the breast-feeding experience, intervention mothers were given a 20-minute audio cassette tape based on relaxation and visual imagery techniques. At a single follow-up expression of milk at the hospital approximately 1 week after enrollment, they expressed 63% more breast milk than a randomized group of control mothers. The fat content of the breast milk in the two groups was not significantly different. Among a small group of mothers whose infants were receiving mechanical ventilation, the increase in milk volume compared with that of control mothers was 121%. Longer-term effects of the relaxation/imagery approach (such as extending the duration of breast-feeding or reducing parental stress after hospital discharge) and the physiologic basis for the increased volume of expressed milk (improved milk production v more efficient milk ejection) are appropriate topics for future research.
|
['Female', 'Humans', 'Imagination', 'Infant, Newborn', 'Infant, Premature', 'Lactation', 'Milk, Human', 'Pregnancy', 'Relaxation Therapy', 'Tape Recording']
| 2,642,620
|
[['B01.050.150.900.649.313.988.400.112.400.400'], ['F02.463.188.634'], ['M01.060.703.520'], ['M01.060.703.520.520'], ['G08.686.523', 'G08.686.702.500'], ['A12.200.467', 'A12.790.500', 'G07.203.100.700.500', 'G07.203.300.350.525.500', 'J02.200.700.500', 'J02.500.350.525.500'], ['G08.686.784.769'], ['E02.190.525.875', 'F04.754.137.750'], ['J01.897.280.500.846', 'L01.178.820.090.846', 'L01.280.940']]
|
['Organisms [B]', 'Psychiatry and Psychology [F]', 'Named Groups [M]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Technology, Industry, and Agriculture [J]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Information Science [L]']
| 1
| 1
| 0
| 0
| 1
| 1
| 1
| 0
| 0
| 1
| 1
| 1
| 0
| 0
|
Persulfonylation of amines applied to the synthesis of higher generation dendrimers.
|
Systematic analysis of the persulfonylation of branched aromatic oligoamines with different arylsulfonyl chlorides allowed optimization of the repetitive steps involved in the synthesis of the sulfonimide-based dendrimers. The optimized procedures afforded the fourth generation N- and pentaphenylene-centered dendrimers with 16 and 32 peripheral groups, respectively. Analysis of products of incomplete substitution showed that the amino groups in aromatic oligoamines are persulfonylated consecutively.
|
['Amines', 'Dendrimers', 'Molecular Structure', 'Sulfur Compounds']
| 18,358,000
|
[['D02.092'], ['D05.750.327', 'E02.319.300.380.200', 'J01.637.512.600.200'], ['G02.111.570', 'G02.466'], ['D01.875', 'D02.886']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Technology, Industry, and Agriculture [J]', 'Phenomena and Processes [G]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Normotension, ventricular tachycardia and palpitation: an unusual presentation of phaeochromocytoma.
|
Phaeochromocytomas are rare catecholamine-producing tumours arising from the cells of the sympathetic nervous system. They account for less than one per cent of hypertension. Undiagnosed sufferers are usually hypertensive and experience episodes of hypertensive crisis. Patients presenting with hypertensive attacks only at intervals and who are at other times normotensive account for only one to five per cent of cases. These subjects have been found to predominantly secrete adrenaline from their tumours. We report here such a case in a 43-year-old man who initially presented to the Coronary Care Unit.
|
['Adrenal Gland Neoplasms', 'Adult', 'Arrhythmias, Cardiac', 'Blood Pressure', 'Humans', 'Male', 'Pheochromocytoma', 'Tachycardia']
| 2,590,629
|
[['C04.588.322.078', 'C19.053.347', 'C19.344.078'], ['M01.060.116'], ['C14.280.067', 'C23.550.073'], ['E01.370.600.875.249', 'G09.330.380.076'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C04.557.465.625.650.700.725', 'C04.557.580.625.650.700.725'], ['C14.280.067.845', 'C14.280.123.875', 'C23.550.073.845']]
|
['Diseases [C]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Ethics of esthetic dentistry.
|
Patient demand for esthetics has increased globally, and often for reasons of patient self-esteem. However, important ethical issues encompass treatment for purely esthetic reasons. Also, perceptions of what is esthetic differ among patients and clinicians. Therefore, the aim of this article is to make suggestions regarding some of the issues surrounding the ethical, esthetic treatment of patients, as well as present three cases illustrating the different meanings of esthetic health to different people.
|
['Adult', 'Esthetics, Dental', 'Ethics, Dental', 'Female', 'Humans', 'Informed Consent', 'Insurance, Dental', 'Retreatment', 'Self Concept', 'Terminology as Topic']
| 15,202,591
|
[['M01.060.116'], ['E06.420'], ['K01.752.566.479.171.132.500', 'K01.752.622.416', 'N05.350.340.162.249'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['I01.880.604.473.650.718', 'I01.880.604.583.427', 'N03.706.437.650.312', 'N03.706.535.489'], ['N03.219.521.576.343.450'], ['E02.887'], ['F01.752.747.792'], ['L01.559.598.400']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Humanities [K]', 'Health Care [N]', 'Organisms [B]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Psychiatry and Psychology [F]', 'Information Science [L]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 1
| 1
| 0
|
Are physiotherapists comfortable with person-centred practice? An autoethnographic insight.
|
PURPOSE: This study aimed to understand our shared conflicting response and discomfort to person-centred rehabilitation within the context of our physiotherapy rehabilitation culture by reflecting on our own experiences as research physiotherapists and clinicians.METHOD: This study used autoethnographical methods to explore the personal and professional experiences of two physiotherapists in neurological rehabilitation. Data were collected through ten written reflections and five joint discussions. The data were analysed collaboratively through focused conversations and writing. We looked for patterns in our data and the literature to triangulate our findings. Joint narratives were structured based on three headings: Where we have come from, Challenges to our position and Where we are now.RESULTS: The four main topics of discussion were goal setting, hope, the physiotherapy paradigm and person-centred practice. Physiotherapy practice is typically underpinned by a biomechanical discourse, which separates the mind and the body. This paradigm limits our ability to manage aspects of person-centred practice, such as valuing patient preferences, fostering hope, managing expectation and building a positive therapeutic relationship.CONCLUSION: Awareness of existing influences on theory and practice is necessary to move the physiotherapy profession towards a greater degree of understanding and application of the principles of person-centred practice. Implications for Rehabilitation Physiotherapists need to recognise that our clinical practice is currently dominated by a biomechanical perspective, which limits our adoption of person-centred practice. Our usual way of working as an expert focuses on our own perspective that makes it difficult to work in a person-centred way. Strategies to incorporate a more person-centred approach include using communication strategies that help us actively seek patients' perspectives.
|
['Attitude of Health Personnel', 'Data Collection', 'Goals', 'Humans', 'Organizational Culture', 'Patient-Centered Care', 'Physical Therapists']
| 23,713,969
|
[['F01.100.050', 'N05.300.100'], ['E05.318.308', 'L01.399.250', 'N05.715.360.300', 'N06.850.520.308'], ['F01.658.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['N04.452.606'], ['N04.590.233.727.407'], ['M01.526.485.790', 'N02.360.790']]
|
['Psychiatry and Psychology [F]', 'Health Care [N]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Information Science [L]', 'Organisms [B]', 'Named Groups [M]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
| 0
|
Dermatoglyphics in Cuban mongols.
|
Finger and palm prints of 220 Cuban mongols, all trisomic 21, were statistically compared with those of a group of 400 normal Cuban individuals. The most important dermatoglyphic findings in the patients were: an excess of ulnar loops on the 2nd and 3rd fingers, and radial loops on the 4th and 5th fingers; a paucity of patterns in the thenar/I area; more hypothenar true patterns, specially ulnar loops, higher percentages of patterns in the II and III interdigital spaces with less true patterns in the IV interdigital space; distal position t" of the axial triradius and large atd angles of more than 70 degrees; high values of the main-line index; predominance of radial type of the C line and higher frequencies of type 11 of the D line. The simian crease, especially the complete variety, had more diagnostic value than the Sydney line. Parathenar patterns which did not seem to have been described previously in mongols, were significantly more frequent in the patients than in controls. The results are very similar to those observed in other countries and it seems that the peculiar dermatoglyphic pattern of patients with Down syndrome is not affected by ethnic influences.
|
['Adolescent', 'Adult', 'Child', 'Child, Preschool', 'Cuba', 'Dermatoglyphics', 'Down Syndrome', 'Female', 'Humans', 'Infant', 'Male', 'Sex Factors']
| 6,451,140
|
[]
|
[]
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Remissions in hairy-cell leukemia with pentostatin (2'-deoxycoformycin).
|
The Eastern Cooperative Oncology Group conducted a study of pentostatin (2'-deoxycoformycin) in 37 patients with hairy-cell leukemia. Among the 27 patients who met all the study's entry criteria, the response rate was 96 percent, with 16 patients (59 percent) entering complete remission and 10 patients (37 percent) partial remission. In one patient no response was observed. These results were not significantly changed by the inclusion of nine additional patients who were found retrospectively not to have fulfilled the entry criteria. When complete remission was attained, maintenance therapy was not given. Despite this, no patient has had a relapse, and the duration of complete remission ranges from 1 to 375 days. Pentostatin appears to be equally effective in untreated patients and in those who have progressive disease after splenectomy or after both splenectomy and treatment with interferon. Whether pentostatin is superior to splenectomy or interferon as therapy for hairy-cell leukemia will have to be assessed by direct comparison in randomized studies. Lengthy follow-up will be required to determine a median duration for the responses of hairy-cell leukemia to pentostatin.
|
['Antineoplastic Agents', 'Coformycin', 'Drug Evaluation', 'Female', 'Humans', 'Interferons', 'Leukemia, Hairy Cell', 'Male', 'Pentostatin', 'Remission Induction', 'Ribonucleosides', 'Splenectomy']
| 2,434,850
|
[['D27.505.954.248'], ['D03.383.742.680.350.200', 'D13.570.685.350.200', 'D13.570.800.410.200'], ['E05.290.625', 'E05.337.425'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.644.276.374.440', 'D12.776.467.374.440', 'D23.529.374.440'], ['C04.557.337.415', 'C15.604.515.553', 'C20.683.515.517'], ['D03.383.742.680.350.200.700', 'D13.570.230.677', 'D13.570.685.350.200.700'], ['E02.860'], ['D13.570.800'], ['E04.726']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Trade-offs among catch, bycatch, and landed value in the American Samoa longline fishery.
|
The interspecific preferences of fishes for different depths and habitats suggest fishers could avoid unwanted catches of some species while still effectively targeting other species. In pelagic longline fisheries, albacore (Thunnus alalunga) are often caught in relatively cooler, deeper water (>100 m) than many species of conservation concern (e.g., sea turtles, billfishes, and some sharks) that are caught in shallower water (<100 m). From 2007 to 2011, we examined the depth distributions of hooks for 1154 longline sets (3,406,946 hooks) and recorded captures by hook position on 2642 sets (7,829,498 hooks) in the American Samoa longline fishery. Twenty-three percent of hooks had a settled depth <100 m. Individuals captured in the 3 shallowest hook positions accounted for 18.3% of all bycatch. We analyzed hypothetical impacts for 25 of the most abundant species caught in the fishery by eliminating the 3 shallowest hook positions under scenarios with and without redistribution of these hooks to deeper depths. Distributions varied by species: 45.5% (n = 10) of green sea turtle (Chelonia mydas), 59.5% (n = 626) of shortbill spearfish (Tetrapturus angustirostris), 37.3% (n = 435) of silky shark (Carcharhinus falciformis), and 42.6% (n = 150) of oceanic whitetip shark (C. longimanus) were caught on the 3 shallowest hooks. Eleven percent (n = 20,435) of all tuna and 8.5% (n = 10,374) of albacore were caught on the 3 shallowest hooks. Hook elimination reduced landed value by 1.6-9.2%, and redistribution of hooks increased average annual landed value relative to the status quo by 5-11.7%. Based on these scenarios, redistribution of hooks to deeper depths may provide an economically feasible modification to longline gear that could substantially reduce bycatch for a suite of vulnerable species. Our results suggest that this method may be applicable to deep-set pelagic longline fisheries worldwide.
|
['American Samoa', 'Animals', 'Conservation of Natural Resources', 'Endangered Species', 'Fisheries', 'Fishes', 'Species Specificity', 'Tuna']
| 24,628,499
|
[['Z01.639.760.815.800.100'], ['B01.050'], ['J01.256', 'N06.230.080'], ['B01.050.050.565', 'G16.500.275.157.049.250', 'N06.230.080.200', 'N06.230.124.049.250'], ['J01.040.168.300', 'J03.540.280'], ['B01.050.150.900.493'], ['G16.824'], ['B01.050.150.900.493.602.825']]
|
['Geographicals [Z]', 'Organisms [B]', 'Technology, Industry, and Agriculture [J]', 'Health Care [N]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
|
N-acetylcysteine ameliorates carbofuran-induced alterations in lipid composition and activity of membrane bound enzymes.
|
The present work investigates the protective effects of N-acetylcysteine (NAC) on carbofuran-induced alterations in lipid composition and activity of membrane bound enzymes (Na+-K+-ATPase and Ca2+-ATPase) in the rat brain. Animals were exposed to carbofuran at a dose of 1 mg/kg body weight, orally, for a period of 28 days. A significant increase in lipid peroxidation in terms of TBARS was observed in brain after carbofuran exposure. NAC administration (200 mg/kg body weight) on the other hand lowered the carbofuran-induced lipid peroxidation to near normal. The increased lipid peroxidation following carbofuran exposure was accompanied by a significant decrease in the levels of total lipids, which is attributed to the reduction in phospholipid levels. Furthermore, NAC administration had a beneficial effect on carbofuran-induced alterations in lipid composition. The ratio of cholesterol to phospholipid, a major determinant of membrane fluidity, was increased in response to carbofuran exposure. This was associated with decreased activity of Na+-K+-ATPase and Ca2+-ATPase. NAC was observed to offer protection by restoring the cholesterol to phospholipid ratio along with the activity of Na+-K+-ATPase and Ca2+-ATPase. The results clearly suggest that carbofuran exerts its neurotoxic effects by increasing lipid peroxidation, altering lipid composition and activity of membrane bound enzymes. NAC administration ameliorated the effects of carbofuran suggesting its potential therapeutic effects in carbofuran neurotoxicity.
|
['Acetylcysteine', 'Animals', 'Body Weight', 'Calcium-Transporting ATPases', 'Carbofuran', 'Cerebral Cortex', 'Cholesterol', 'Free Radical Scavengers', 'Insecticides', 'Lipid Metabolism', 'Lipid Peroxidation', 'Male', 'Phospholipids', 'Rats', 'Rats, Wistar', 'Sodium-Potassium-Exchanging ATPase']
| 16,496,214
|
[['D02.886.030.230.259', 'D12.125.166.230.259'], ['B01.050'], ['C23.888.144', 'E01.370.600.115.100.160.120', 'E05.041.124.160.750', 'G07.100.100.160.120', 'G07.345.249.314.120'], ['D08.811.277.040.025.314.250', 'D12.776.157.530.450.250.500', 'D12.776.157.530.813.250', 'D12.776.543.585.450.250.500', 'D12.776.543.585.813.250'], ['D02.241.081.251.583.166'], ['A08.186.211.200.885.287.500'], ['D04.210.500.247.222.284', 'D04.210.500.247.808.197', 'D10.570.938.208'], ['D27.505.519.217.500'], ['D27.720.031.700.491', 'D27.888.723.491'], ['G03.458'], ['G02.111.515', 'G03.295.531.587'], ['D10.570.755'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.900'], ['D08.811.277.040.025.314.750', 'D12.776.157.530.450.162.780', 'D12.776.157.530.450.250.880', 'D12.776.157.530.813.750', 'D12.776.543.585.450.162.800', 'D12.776.543.585.450.250.890', 'D12.776.543.585.813.750']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Neurexin 3 polymorphisms are associated with alcohol dependence and altered expression of specific isoforms.
|
Neurexins are cell adhesion molecules that help to specify and stabilize synapses and provide receptors for neuroligins, neurexophilins, dystroglycans and alpha-latrotoxins. We previously reported significant allele frequency differences for single nucleotide polymorphisms (SNPs) in the neurexin 3 (NRXN3) gene in each of two comparisons between individuals who were dependent on illegal substances and controls. We now report work clarifying details of NRXN3's gene structure and variants and documenting association of NRXN3 SNPs with alcohol dependence. We localize this association signal with the vicinity of the NRXN3 splicing site 5 (SS#5). A splicing site SNP, rs8019381, that is located 23 bp from the SS#5 exon 23 donor site displays association with P = 0.0007 (odds ratio = 2.46). Including or excluding exon 23 at SS#5 produces soluble or transmembrane NRXN3 isoforms. We thus examined expression of these NRXN3 isoforms in postmortem human cerebral cortical brain samples from individuals with varying rs8019381 genotypes. Two of the splice variants that encode transmembrane NRXN3 isoforms were expressed at significantly lower levels in individuals with the addiction-associated rs8019381 'T' allele than in CC homozygotes. Taken together with recent reports of NRXN3 association with nicotine dependence and linkage with opiate dependence, these data support roles for NRXN3 haplotypes that alter expression of specific NRXN3 isoforms in genetic vulnerabilities to dependence on a variety of addictive substances.
|
['Adult', 'Aged', 'Aged, 80 and over', 'Alcoholism', 'Alleles', 'Alternative Splicing', 'Base Sequence', 'Case-Control Studies', 'Cell Adhesion Molecules, Neuronal', 'Cerebral Cortex', 'DNA Primers', 'Exons', 'Female', 'Gene Expression', 'Genotype', 'Haplotypes', 'Humans', 'Introns', 'Male', 'Middle Aged', 'Nerve Tissue Proteins', 'Polymorphism, Single Nucleotide', 'Protein Isoforms', 'RNA, Messenger']
| 17,804,423
|
[['M01.060.116'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['C25.775.100.250', 'F03.900.100.350'], ['G05.360.340.024.340.030'], ['G02.111.760.700.100', 'G03.839.700.100', 'G05.308.700.700.100'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['D12.776.395.550.200.250', 'D12.776.543.550.200.250', 'D23.050.301.350.250'], ['A08.186.211.200.885.287.500'], ['D13.695.578.424.450.275', 'D27.720.470.530.600.223.600'], ['G05.360.340.024.340.137.232'], ['G05.297'], ['G05.380'], ['G05.380.360'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G05.360.340.024.220.400', 'G05.360.340.024.340.137.515'], ['M01.060.116.630'], ['D12.776.631'], ['G05.365.795.598'], ['D12.776.800'], ['D13.444.735.544']]
|
['Named Groups [M]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Organisms [B]']
| 1
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 1
| 1
| 1
| 0
|
Vagus nerve stimulation blocks vascular permeability following burn in both local and distal sites.
|
Recent studies have shown that vagus nerve stimulation (VNS) can block the burn-induced systemic inflammatory response (SIRS). In this study we examined the potential for VNS to modulate vascular permeability (VP) in local sites (i.e. skin) and in secondary sites (i.e. lung) following burn. In a 30% total body surface area burn model, VP was measured using intravascular fluorescent dextran for quantification of the VP response in skin and lung. A peak in VP of the skin was observed 24h post-burn injury, that was blocked by VNS. Moreover, in the lung, VNS led to a reduction in burn-induced VP compared to sham-treated animals subjected to burn alone. The protective effects of VNS in this model were independent of the spleen, suggesting that the spleen was not a direct mediator of VNS. These studies identify a role for VNS in the regulation of VP in burns, with the translational potential of attenuating lung complications following burn.
|
['Animals', 'Burns', 'Capillary Permeability', 'Dextrans', 'Disease Models, Animal', 'Fluorescent Dyes', 'Lung', 'Mice', 'Mice, Inbred BALB C', 'Skin', 'Vagus Nerve Stimulation']
| 22,694,873
|
[['B01.050'], ['C26.200'], ['G03.143.330', 'G09.330.165'], ['D05.750.078.562.272', 'D09.698.365.272'], ['C22.232', 'E05.598.500', 'E05.599.395.080'], ['D27.720.233.348', 'D27.720.470.410.505.500'], ['A04.411'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.338', 'B01.050.150.900.649.313.992.635.505.500.400.338'], ['A17.815'], ['E02.331.900']]
|
['Organisms [B]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Influence of tetraazamacrocyclic ligands on the nitric oxide reactivity of their cobalt(II) complexes.
|
The reactions of cobalt(II) complexes of tetraazamacrocyclic tropocoronand (TC) ligands with nitric oxide (NO) were investigated. When [Co(TC-5,5)] was allowed to react with NO(g), the {CoNO}(8) mononitrosyl [Co(NO)(TC-5,5)] was isolated and structurally characterized. In contrast, a {Co(NO)(2)}(10) species formed when [Co(TC-6,6)] was exposed to NO(g), and the nitrito [Co(NO(2))(TC-6,6)] complex was structurally and spectroscopically characterized from the reaction mixture. The {Co(NO)(2)}(10) species was assigned as the bis(cobalt dinitrosyl) complex [Co(2)(NO)(4)(TC-6,6)] by spectroscopic comparison with independently synthesized and characterized material. These results provide the first evidence for the influence of tropocoronand ring size on the nitric oxide reactivity of the cobalt(II) complexes.
|
['Aza Compounds', 'Cobalt', 'Coordination Complexes', 'Ligands', 'Macrocyclic Compounds', 'Models, Molecular', 'Nitric Oxide', 'Spectrophotometry, Infrared']
| 22,742,582
|
[['D02.145'], ['D01.268.556.185', 'D01.268.956.155', 'D01.552.544.185'], ['D01.234', 'D02.257'], ['D27.720.470.480'], ['D04.345'], ['E05.599.595'], ['D01.339.387', 'D01.625.550.500', 'D01.625.700.500', 'D01.650.550.587.600'], ['E05.196.712.726.676', 'E05.196.867.826.676']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Toward an understanding of anticipatory pleasure deficits in schizophrenia: Memory, prospection, and emotion experience.
|
Anticipatory pleasure deficits have been observed in people with schizophrenia. Less is known about the extent to which interrelated processes that comprise anticipatory pleasure, including memory, prospection, and emotion experience are disrupted. We asked people with (n = 32) and without (n = 29) schizophrenia or schizoaffective disorder to provide memory and prospection narratives in response to specific cues. Half of the prospections followed a memory task, and half followed a control task. People with schizophrenia generated memories similar in content and experience as controls even as they described them less clearly. However, people with schizophrenia were less likely to explicitly reference the past in their prospections, and their prospections were less detailed and richly experienced than controls, regardless of the task completed before prospection. People with schizophrenia reported similar levels of positive emotion (current and predicted) in positive prospections that followed the memory task, but less positive emotion than controls in positive prospections that followed the control task. Taken together, these results suggest that people with schizophrenia experience difficulties drawing from past experiences and generating detailed prospections. However, asking people with schizophrenia to recall and describe memories prior to prospection may increase the likelihood of drawing from the past in prospections, and may help boost current and predicted pleasure.
|
['Adult', 'Anhedonia', 'Female', 'Humans', 'Male', 'Memory', 'Memory, Episodic', 'Middle Aged', 'Neuropsychological Tests', 'Pleasure', 'Schizophrenic Psychology']
| 26,950,753
|
[['M01.060.116'], ['C10.597.606.057', 'C23.888.592.604.039', 'F01.700.039'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F02.463.425.540'], ['F02.463.425.540.254'], ['M01.060.116.630'], ['F04.711.513'], ['F01.470.867', 'F02.830.816.492'], ['F04.824']]
|
['Named Groups [M]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
A direct binding assay for the vascular cell adhesion molecule-1 (VCAM1) interaction with alpha 4 integrins.
|
Vascular cell adhesion molecule-1 (VCAM1) is a member of the immunoglobulin (Ig) superfamily which interacts with the alpha 4 integrins alpha 4 beta 1 (very late antigen 4: VLA4) and alpha 4 beta 7, which are constitutively expressed on many leukocyte subsets and play a key role in cell trafficking and activation. Using a recombinant VCAM-IgG fusion protein (VCAM-Ig) as a soluble ligand for alpha 4 beta 1 we directly demonstrated by fluorescence analysis that the alpha 4 beta 1 receptor can exist in different affinity states on the cell surface, and that a high affinity state is induced by manganese ions or certain activating anti-beta 1 monoclonal antibodies (Jakubowski et al., 1995b). Here we have extended these observations by developing a rapid and reproducible assay using alkaline phosphatase (AP)-coupled VCAM-Ig (VCAM-Ig-AP) which measures the interaction between VCAM1 and alpha 4 integrins in a microtiter plate format. This assay has allowed us to evaluate directly the effects of metal ions, anti-beta 1 mAbs, and different cell types and species on the VCAM1/alpha 4 integrin interaction. Most importantly, the assay system provides a means to rapidly evaluate alpha 4 integrin-directed inhibitors without the complication of post-ligand binding events inherent in adhesion assays.
|
['Animals', 'Antibodies, Monoclonal', 'Antigens, CD', 'Binding, Competitive', 'Cell Adhesion', 'Fluorescent Antibody Technique', 'Humans', 'Integrin alpha4', 'Integrins', 'Leukemia', 'Magnesium', 'Mice', 'Protein Binding', 'Rats', 'Tumor Cells, Cultured', 'Vascular Cell Adhesion Molecule-1']
| 8,640,376
|
[['B01.050'], ['D12.776.124.486.485.114.224', 'D12.776.124.790.651.114.224', 'D12.776.377.715.548.114.224'], ['D23.050.301.264.035', 'D23.101.100.110'], ['E05.196.080', 'G02.111.084', 'G02.111.570.120.309'], ['G04.022'], ['E01.370.225.500.607.512.240', 'E01.370.225.750.551.512.240', 'E05.200.500.607.512.240', 'E05.200.750.551.512.240', 'E05.478.583.375'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.776.543.750.705.408.100.450'], ['D12.776.543.750.705.408'], ['C04.557.337'], ['D01.268.552.437', 'D01.268.557.500', 'D01.552.547.500'], ['B01.050.150.900.649.313.992.635.505.500'], ['G02.111.679', 'G03.808'], ['B01.050.150.900.649.313.992.635.505.700'], ['A11.251.860'], ['D12.776.395.550.200.920', 'D12.776.543.550.200.920', 'D23.050.301.350.920']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Comparison of the lateral flow immunoassays (LFIA) for the diagnosis of Helicobacter pylori infection.
|
Helicobacter pylori infection is the most common human infection where approximately 50% of the world populations are infected. The diagnosis of such infection is mainly done by endoscopy where gastric biopsies are examined for the presence of H. pylori. Such invasive approach is costly, time consuming and generally requires more than one test to confirm the infection. Serology on the other hand is a non-invasive approach that can detect H. pylori exposure. The lateral flow immunoassays (LFIA) support the serological approach and have the advantage of being fast, economic and require no additional equipment or experience. In this review the principles, components of the LFIA, sensitivities and specificities of the commercially available H. pylori test strips were compared and discussed.
|
['Antibodies', 'Feces', 'Gastrointestinal Diseases', 'Gastroscopy', 'Helicobacter Infections', 'Helicobacter pylori', 'Humans', 'Immunoassay', 'Sensitivity and Specificity', 'Urea']
| 23,994,110
|
[['D12.776.124.486.485.114', 'D12.776.124.790.651.114', 'D12.776.377.715.548.114'], ['A12.459'], ['C06.405'], ['E01.370.372.250.250.325', 'E01.370.388.250.250.250.320', 'E04.210.240.250.320', 'E04.502.250.250.250.320'], ['C01.150.252.400.466'], ['B03.440.500.550', 'B03.660.150.235.500.250.550'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.478.566', 'E05.601.470'], ['E05.318.370.800', 'E05.318.740.872', 'G17.800', 'N05.715.360.325.700', 'N05.715.360.750.725', 'N06.850.520.445.800', 'N06.850.520.830.872'], ['D02.065.950']]
|
['Chemicals and Drugs [D]', 'Anatomy [A]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Health Care [N]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
A study of the mechanisms of cytotoxicity of Ara-C on three human leukemic cell lines.
|
The main biochemical determinants involved in cytosine arabinoside (Ara-C) metabolism were studied in one lymphoblastic (Reh) and two myeloid (HL60 and K562) human leukemic cell lines exhibiting various sensitivities to Ara-C, Reh being the most and HL60 the least sensitive. The level of intracellular Ara-C accumulation and Ara-CTP formation was far more important in Reh cells than in myeloid cell lines but was not closely related to deoxycytidine kinase activity or to deoxycytidine triphosphate pool size. The level of Ara-C incorporated into DNA was similar in the three cell lines. Ara-CTP formation correlated better with the cytotoxicity to clonogenic cells than did Ara-C incorporation into DNA. DNA polymerase alpha was moderately inhibited to various degrees, depending on the cell line; this moderate inhibition does not seem sufficient to explain the inhibition of DNA synthesis. The activity of DNA ligase, the enzyme joining the Okazaki fragments, which was not detected in Reh cells, was strongly inhibited by Ara-C in HL60 and to a lesser degree, in K562 cells. The inhibition of DNA ligase probably also contributes to the inhibition of DNA synthesis and, thus, to the cytotoxic effect of Ara-C and may explain the smaller size of DNA fragments observed following Ara-C treatment. The variations in each critical determinant observed in these three cell lines increase the complexity and plurality of the mechanisms of Ara-C action.
|
['Arabinofuranosylcytosine Triphosphate', 'Cytarabine', 'DNA', 'DNA Ligases', 'DNA Polymerase II', 'DNA, Neoplasm', 'Deoxycytidine Kinase', 'Deoxycytosine Nucleotides', 'Humans', 'Leukemia', 'Leukemia, Myelogenous, Chronic, BCR-ABL Positive', 'Leukemia, Promyelocytic, Acute', 'Precursor Cell Lymphoblastic Leukemia-Lymphoma', 'Tumor Cells, Cultured', 'Tumor Stem Cell Assay']
| 2,752,506
|
[['D03.383.742.686.246.050', 'D13.695.065.200', 'D13.695.740.246.050'], ['D03.383.742.680.245.453', 'D13.570.065.300', 'D13.570.685.245.453'], ['D13.444.308'], ['D08.811.074.500', 'D08.811.464.754.600'], ['D08.811.913.696.445.308.300.230'], ['D13.444.308.425'], ['D08.811.913.696.620.175'], ['D03.383.742.686.246.425', 'D13.695.201.150', 'D13.695.740.246.425'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C04.557.337'], ['C04.557.337.539.250', 'C15.378.190.636.370'], ['C04.557.337.539.275.700'], ['C04.557.337.428.600', 'C15.604.515.560.600', 'C20.683.515.528.600'], ['A11.251.860'], ['E01.370.225.500.383.910', 'E01.370.225.500.388.930', 'E05.200.500.383.910', 'E05.200.500.388.930', 'E05.242.383.910', 'E05.242.417.500', 'E05.337.550.200.800']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Diseases [C]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
ferT encodes a meiosis-specific nuclear tyrosine kinase.
|
ferT is a mouse testis-specific mRNA, shown previously to potentially encode a 51 kilodalton tyrosine kinase termed p51ferT. The accumulation of ferT RNA is restricted to primary spermatocytes that are at the prophase stage of the first spermatogenic meiotic division. By using antibodies raised against a synthetic peptide which was designed according to a putative p51ferT unique amino acid sequence, we have shown that testicular cells indeed contain a 51 kilodalton protein that is recognized by the anti-p51ferT antibodies. The protein was not detected in six nontesticular mouse tissues, nor was it detected, like the ferT RNA, in the testes of 14-day-old mice. These findings strongly suggest that the 51 kilodalton protein is p51ferT. Immunohistochemical staining localized p51ferT to meiotically dividing spermatocytes. Transfection experiments in CHO cells confirmed the nuclear localization of p51ferT in eukaryotic cells. p51ferT seems thus to be the first meiosis-specific nuclear tyrosine kinase described to date.
|
['Animals', 'CHO Cells', 'Cricetinae', 'Male', 'Meiosis', 'Mice', 'Nuclear Matrix', 'Nuclear Proteins', 'Organ Specificity', 'Peptide Fragments', 'Prophase', 'Protein-Tyrosine Kinases', 'Recombinant Fusion Proteins', 'Spermatocytes', 'Transfection']
| 8,373,729
|
[['B01.050'], ['A11.251.210.200', 'A11.436.155'], ['B01.050.150.900.649.313.992.635.075.250'], ['G04.144.220.220.687', 'G05.113.220.687'], ['B01.050.150.900.649.313.992.635.505.500'], ['A11.284.430.106.279.345.700'], ['D12.776.660'], ['G07.650'], ['D12.644.541'], ['G04.144.220.220.687.883', 'G04.144.220.220.781.906', 'G05.113.220.687.883', 'G05.113.220.781.906'], ['D08.811.913.696.620.682.725'], ['D12.776.828.300'], ['A05.360.490.890.880', 'A11.497.760.700'], ['E05.393.350.810', 'G05.728.860']]
|
['Organisms [B]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Reduction in parenteral nutrition related complications in the newborn.
|
A comparison of total parenteral nutrition (TPN) related complication in newborns was made between two study periods, namely, 1986 (Study A) and 1989-90 (Study B). A significant reduction was seen in all complications in Study B. Local complications (thrombophlebitis, gangrene, abscess) reduced from 80.0 to 29.4%, septicemia from 52.0 to 11.7% and metabolic complications from a computed mean of 1.6 episode per baby to 0.88 episode per baby. The reduction in these complications has been attributed to the following additional inputs in the recent study (i) Additional staff (research officers, nurses, biochemist); (ii) Better training of resident staff; (iii) Use of a laminar flow system for mixing solutions; (iv) Specially designed locally manufactured intravenous sets and accessories; and (v) Use of well balanced nutrient solutions. Outstanding problems perceived are--high incidence of TPN-related cholestasis (14.7%), azotemia (26.4%), central catheter-related sepsis (75.0%) and the falling, but yet high cost of the technique (Rs. 650 per day).
|
['Humans', 'India', 'Infant Nutritional Physiological Phenomena', 'Infant, Newborn', 'Infusions, Intravenous', 'Intensive Care Units, Neonatal', 'Intensive Care, Neonatal', 'Parenteral Nutrition', 'Skin', 'Skin Diseases, Infectious', 'Thrombophlebitis', 'Water-Electrolyte Imbalance']
| 1,752,674
|
[['B01.050.150.900.649.313.988.400.112.400.400'], ['Z01.252.245.393'], ['G07.203.650.220.500'], ['M01.060.703.520'], ['E02.319.267.082.500', 'E02.319.267.510.590'], ['N02.278.388.493.390.380'], ['E02.760.190.405', 'N02.421.585.190.500'], ['E02.421.505', 'E02.642.500.505'], ['A17.815'], ['C01.800', 'C17.800.838'], ['C14.907.355.830.925.770', 'C14.907.617.718.788', 'C14.907.940.740.910'], ['C18.452.950']]
|
['Organisms [B]', 'Geographicals [Z]', 'Phenomena and Processes [G]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Anatomy [A]', 'Diseases [C]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Conjoined lumbosacral nerve roots. Management of herniated discs and lateral recess stenosis in patients with this anomaly.
|
Anomalous L-5 and S-1 nerve roots occur infrequently. If not properly recognized, surgery for entrapment disorders may result in serious neural injury because of an improper surgical approach in exposure and in removing the underlying herniated discs. The diagnosis has been made preoperatively since the introduction of water-soluble myelography because of improved filling of the nerve roots. A herniated disc beneath the bifid root causes extreme pain and disability with marked signs of entrapment because of firm fixation of the conjoined root in the lateral recess between the two pedicles. An underlying herniated disc may not be recognized because of the unique anatomical changes. To properly identify the nature of the lesion, wide exposure by hemilaminectomy is preferred, with unroofing of the lateral recesses and wide foraminal decompression. Eight such patients are reported: seven had herniated discs, and one had lateral recess stenosis with superior facet entrapment. With adequate decompression, all patients made a rapid, uneventful recovery.
|
['Adult', 'Female', 'Humans', 'Intervertebral Disc Displacement', 'Laminectomy', 'Lumbosacral Region', 'Male', 'Metrizamide', 'Middle Aged', 'Myelography', 'Nerve Compression Syndromes', 'Spinal Nerve Roots']
| 7,277,006
|
[['M01.060.116'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C05.116.900.307', 'C23.300.707.952'], ['E02.718.563', 'E04.188.400', 'E04.525.450', 'E04.555.350'], ['A01.923.176.519'], ['D02.241.223.100.400.880.520', 'D02.455.426.559.389.127.375.880.520', 'D09.408.051.545'], ['M01.060.116.630'], ['E01.370.350.578.937.505', 'E01.370.350.700.560.505', 'E01.370.376.537.750.505', 'E05.629.937.505'], ['C10.668.829.550'], ['A08.800.800.720.725']]
|
['Named Groups [M]', 'Organisms [B]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Chemicals and Drugs [D]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Plasma brain natriuretic peptide is a biochemical marker for the prediction of progressive ventricular remodeling after acute myocardial infarction.
|
To investigate the relation between plasma brain natriuretic peptide (BNP) and progressive ventricular remodeling, we measured plasma BNP and atrial natriuretic peptide (ANP) in 30 patients with acute myocardial infarction on days 2, 7, 14, and 30 after the onset. Left ventricular end-diastolic volume index (EDVI), end-systolic volume index (ESVI), and ejection fraction (EF) on admission and 1 month after the onset were assessed by left ventriculography. Changes in EDVI (deltaEDVI), ESVI (deltaESVI), and EF (deltaEF) were obtained by subtracting respective acute-phase values from corresponding chronic-phase values. Plasma ANP on days 2 and 7 showed only weak correlations with deltaEDVI (r = 0.48 and 0.54; both p < 0.01), whereas plasma BNP on day 7 more closely correlated with deltaEDVI (r = 0.77; p < 0.001). When study patients were divided into two groups according to plasma BNP on day 7, the group with BNP higher than 100 pg/ml showed greater increases in left ventricular volume and less improvement in EF compared with the other group with BNP lower than 100 pg/ml (deltaEDVI = 10.4 +/- 8 vs -3.4 +/- 9 ml/m2, deltaESVI = 6.2 +/- 7 vs -4.9 +/- 5 ml/m2, and deltaEF = 1.0% +/- 4% vs 4.9% +/- 5%; p < 0.05, respectively). Multiple regression analysis revealed that only plasma BNP on day 7, but not ANP, peak creatine phosphokinase level, left ventricular end-diastolic pressure, or acute-phase EF, correlated independently with deltaEDVI (p < 0.01). These results suggest that plasma BNP may be a simple and useful biochemical marker for the prediction of progressive ventricular remodeling within the first 30 days of acute myocardial infarction.
|
['Adult', 'Aged', 'Aged, 80 and over', 'Atrial Natriuretic Factor', 'Biomarkers', 'Female', 'Hemodynamics', 'Humans', 'Hypertrophy, Left Ventricular', 'Male', 'Middle Aged', 'Myocardial Infarction', 'Natriuretic Peptide, Brain', 'Nerve Tissue Proteins', 'Predictive Value of Tests', 'Ventricular Function, Left']
| 9,453,517
|
[['M01.060.116'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['D06.472.699.584.500', 'D12.644.548.585.500'], ['D23.101'], ['G09.330.380'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C14.280.195.400', 'C23.300.775.250.400'], ['M01.060.116.630'], ['C14.280.647.500', 'C14.907.585.500', 'C23.550.513.355.750', 'C23.550.717.489.750'], ['D06.472.699.584.625', 'D12.644.548.585.625', 'D12.776.631.590'], ['D12.776.631'], ['E05.318.370.800.650', 'N05.715.360.325.700.640', 'N06.850.520.445.800.650'], ['G09.330.955.800']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
The clinical significance of hemoglobinopathies in the Hamilton region: a twenty-year review.
|
Between 1970-1990, the Laboratory tested 38,391 specimens for hemoglobinopathies, of which 7,935 were positive. The major abnormalities detected were beta thalassemia trait (4,688), alpha thalassemia trait (1,248) and sickle cell trait (847). Clinically significant hemoglobinopathies detected were Hemoglobin H disease (100), sickle cell disease (67) and sickle cell Hemoglobin C disease (79). Hemoglobinopathies are therefore common in the Hamilton area as a reflection of the cultural diversity of area citizens. Of the 49 patients with thalassemia without documented iron deficiency, 8 (16%) received iron therapy for a variable period of time and 3 were investigated for gastrointestinal blood loss. Hemoglobin abnormalities cause or have the potential to cause clinical disease and they can, if not detected, result in unnecessary iron therapy or gastrointestinal investigation.
|
['Hemoglobin C Disease', 'Hemoglobin E', 'Hemoglobin SC Disease', 'Hemoglobinopathies', 'Hemoglobins, Abnormal', 'Humans', 'Ontario', 'Sickle Cell Trait', 'alpha-Thalassemia', 'beta-Thalassemia']
| 1,458,712
|
[['C15.378.071.141.150.490', 'C15.378.420.463', 'C16.320.070.490', 'C16.320.365.463'], ['D12.776.124.400.463.375', 'D12.776.422.316.762.426.375'], ['C15.378.071.141.150.150.440', 'C15.378.420.155.440', 'C16.320.070.150.440', 'C16.320.365.155.440'], ['C15.378.420', 'C16.320.365'], ['D12.776.124.400.463', 'D12.776.422.316.762.426'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['Z01.107.567.176.639'], ['C15.378.071.141.150.150.670', 'C15.378.420.155.668', 'C16.320.070.150.670', 'C16.320.365.155.668'], ['C15.378.071.141.150.875.100', 'C15.378.420.826.100', 'C16.320.070.875.100', 'C16.320.365.826.100'], ['C15.378.071.141.150.875.150', 'C15.378.420.826.150', 'C16.320.070.875.150', 'C16.320.365.826.150']]
|
['Diseases [C]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Geographicals [Z]']
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
|
Evidence for theory of mind deficits in euthymic patients with bipolar disorder.
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OBJECTIVE: i) To investigate the subtle ToM (theory of mind) deficits in euthymic patients with bipolar disorder. ii) To investigate the impact of non-ToM cognitive deficits on ToM abilities.METHOD: Forty-three euthymic patients with bipolar disorder and 30 healthy control subjects were involved in this study. ToM was assessed by the Eyes test and the Hinting task. Both groups were also evaluated with a comprehensive neuropsychological battery including tasks for basic emotion and face recognition.RESULTS: The patient group was impaired on both of the ToM tasks. The patient group also showed impairment in many cognitive tasks including tasks related to sustained attention.CONCLUSION: Even euthymic patients with bipolar disorder may be impaired in advanced ToM tasks. Executive dysfunction and some other cognitives deficits such as basic emotion recognition may be at least partly responsible for this result.
|
['Adult', 'Analysis of Variance', 'Attention', 'Bipolar Disorder', 'Cognition Disorders', 'Emotions', 'Face', 'Female', 'Humans', 'Male', 'Neuropsychological Tests', 'Pattern Recognition, Visual', 'Reference Values', 'Social Perception']
| 15,992,392
|
[['M01.060.116'], ['E05.318.740.150', 'N05.715.360.750.125', 'N06.850.520.830.150'], ['F02.830.104.214'], ['F03.084.500'], ['F03.615.250'], ['F01.470'], ['A01.456.505'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F04.711.513'], ['F02.463.593.524.500', 'F02.463.593.932.622'], ['E05.978.810'], ['F02.463.593.752']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Psychiatry and Psychology [F]', 'Anatomy [A]', 'Organisms [B]']
| 1
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
The Kansas LIFE project-living initiatives for end-of-life care.
|
BACKGROUND: Numerous studies continue to demonstrate the needs for improved palliative and end-of-life care.OBJECTIVE: Kansas stakeholders joined together, under the leadership of the Kansas Hospice and Palliative Care Organization (formerly the Association of Kansas Hospices) in 1997, to improve palliative and end-of-life care for Kansans living with advanced chronic and terminal illnesses.DESIGN: The Kansas LIFE Project (Living Initiatives for End-of-Life Care) plans and implements strategic initiatives and projects that engage public policy leaders, healthcare professionals and Kansas citizens.SETTING: Grassroots community coalitions, LIFE Project Caring Communities, have been developed, supported, resourced, and maintained as a core strategic and action tool. for assuring that local and state progress is achieved and meaningful and relevant to Kansas citizens.CONCLUSION: Local community coalitions have the best understanding of citizen needs and are important to include in state-level planning and action in ways that lead to successful, meaningful and relevant outcomes for improved care for citizens living with advanced chronic and terminal illnesses. With adequate support and strong leadership, local coalitions can effectively leverage their presence and "ownership" of communities to drive change among health care professionals, policy leaders, and citizens.
|
['Communication', 'Community Health Planning', 'Cooperative Behavior', 'Health Care Coalitions', 'Humans', 'Kansas', 'Palliative Care', 'Program Evaluation', 'Quality Assurance, Health Care', 'Terminal Care']
| 15,684,852
|
[['F01.145.209', 'L01.143'], ['N03.349.650.185'], ['F01.145.813.115'], ['N03.540.452.188'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['Z01.107.567.875.510.390'], ['E02.760.666', 'N02.421.585.666'], ['E05.337.820', 'N04.761.685', 'N05.715.360.650'], ['N04.761.700', 'N05.700'], ['E02.760.905', 'N02.421.585.905']]
|
['Psychiatry and Psychology [F]', 'Information Science [L]', 'Health Care [N]', 'Organisms [B]', 'Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 1
| 1
|
Effect of premolar and molar occlusal angle on feed digestibility, water balance, and fecal particle size in horses.
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OBJECTIVE: To determine whether occlusal angle of the premolar and molar teeth (ie, molar occlusal angle) was associated with feed digestibility, water balance, or fecal particle size in adult horses.DESIGN: Observational study.ANIMALS: 40 pregnant mares ranging from 3 to 19 years old.PROCEDURE: The horses were randomly allocated to 1 of 5 feeding groups with 8 horses/group. Horses were sedated, and molar occlusal angle was measured with 2 methods. An oral examination was performed, and total number of dental abnormalities was recorded. Feed digestibility, water balance, and fecal particle size were measured 7 and 16 weeks later.RESULTS: Molar occlusal angle ranged from 6.3 degrees to 19.3 degrees and was not significantly associated with feed digestibility, water balance, or fecal particle size. The number of dental abnormalities was not associated with feed digestibility. Molar occlusal angle did not vary significantly with horse age.CONCLUSIONS AND CLINICAL RELEVANCE: Results indicate that molar occlusal angles between 6 degrees and 19 degrees do not adversely affect feed digestibility, water balance, or fecal particle size in adult horses. Additionally, there was no association between age and molar occlusal angle.
|
['Animals', 'Dental Occlusion', 'Dentistry', 'Digestion', 'Feces', 'Female', 'Horses', 'Malocclusion', 'Mastication', 'Molar', 'Particle Size', 'Pregnancy', 'Random Allocation', 'Water-Electrolyte Balance']
| 16,013,545
|
[['B01.050'], ['E06.276', 'G10.549.208'], ['E06', 'H02.163'], ['G07.203.650.250', 'G10.261.190'], ['A12.459'], ['B01.050.150.900.649.313.984.235.472'], ['C07.793.494'], ['G07.203.650.283.500', 'G10.261.330.500'], ['A14.549.167.860.525'], ['G02.712'], ['G08.686.784.769'], ['E05.318.370.700', 'E05.581.500.805', 'N05.715.360.325.675', 'N06.850.520.445.700'], ['G02.111.635.500', 'G03.615.500', 'G07.410.810.500']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Disciplines and Occupations [H]', 'Anatomy [A]', 'Diseases [C]', 'Health Care [N]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
|
Furosemide reveals heterogeneous GABA(A) receptor expression at adult rat Golgi cell to granule cell synapses.
|
The contribution that alpha6 subunit-containing GABA(A) receptors make to inhibitory synaptic transmission to granule cells was investigated by making whole-cell patch clamp recordings from granule cells in adult rat cerebellar slices and applying furosemide, the specific alpha6 subunit-containing GABA(A) receptor antagonist. Endogenous, extracellular GABA continually activated GABA(A) receptors producing a tonic current. Since this current was markedly reduced by furosemide it was probably produced by alpha6 subunit-containing receptors. In contrast, furosemide had little effect on the amplitude or kinetics of fast spontaneous inhibitory postsynaptic currents (sIPSCs), although such sIPSCs were abolished by bicuculline and SR95331. However, the amplitude of evoked IPSCs with a very slow rise and decay were markedly reduced by furosemide. These IPSCs probably resulted from the spillover of GABA from neighbouring synapses activating high affinity alpha6 subunit-containing receptors. In the rest of the cells (40 out of 46), evoked IPSCs had rise and decay kinetics that lay in-between fast sIPSCs and slow 'spillover' IPSCs. Such IPSCs had variable kinetics and also exhibited considerable variation in the magnitude of furosemide block. Thus the GABA(A) receptors present at adult Golgi cell-granule cell synapses, at a developmental stage where receptor expression is complete, are highly heterogeneous.
|
['Animals', 'Cerebellum', 'Cytoplasmic Granules', 'Diuretics', 'Electrophysiology', 'Excitatory Postsynaptic Potentials', 'Furosemide', 'In Vitro Techniques', 'Male', 'Membrane Potentials', 'Patch-Clamp Techniques', 'Rats', 'Rats, Wistar', 'Receptors, GABA-A', 'Synapses', 'Synaptic Transmission', 'gamma-Aminobutyric Acid']
| 12,367,619
|
[['B01.050'], ['A08.186.211.132.810.428.200'], ['A11.284.430.214.190.500', 'A11.284.430.214.190.875.190.190'], ['D27.505.696.560.500'], ['H01.158.344.528', 'H01.158.782.236'], ['G04.580.887.249', 'G07.265.675.887.249', 'G07.265.880.750.199', 'G11.561.570.918.249', 'G11.561.830.750.199'], ['D02.065.884.725.300', 'D02.092.146.807.300', 'D02.886.590.700.725.300'], ['E05.481'], ['G01.154.535', 'G04.580', 'G07.265.675', 'G11.561.570'], ['E05.200.500.905', 'E05.242.800'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.900'], ['D12.776.157.530.400.175.562', 'D12.776.157.530.400.400.100.100', 'D12.776.543.550.450.175.562', 'D12.776.543.550.450.500.100.100', 'D12.776.543.585.400.175.562', 'D12.776.543.585.400.500.100.100', 'D12.776.543.750.130.500', 'D12.776.543.750.720.200.300.300'], ['A08.850', 'A11.284.149.165.420.780'], ['G02.111.820.850', 'G04.835.850', 'G07.265.880', 'G11.561.830'], ['D02.241.081.114.500.350', 'D12.125.190.350']]
|
['Organisms [B]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Disciplines and Occupations [H]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
Trifactorial classification system for osteotome sinus floor elevation based on an observational retrospective analysis of 926 implants followed up to 10 years.
|
OBJECTIVE: A novel osteotome trifactorial classification system is proposed for transcrestal osteotome-mediated sinus floor elevation (OSFE) sites that includes residual bone height (RBH), sinus floor anatomy (contour), and multiple versus single sites OSFE (tenting).METHOD AND MATERIALS: An analysis of RBH, contour, and tenting was retrospectively applied to a cohort of 926 implants placed using OSFE without added bone graft and followed up to 10 years. RBH was divided into three groups: high (RBH > 6 mm), mid (RBH = 4.1 to 6 mm), and low (RBH = 2 to 4 mm). The sinus "contour" was divided into four groups: flat, concave, angle, and septa. For "tenting", single versus multiple adjacent OSFE sites were compared.RESULTS: The prevalence of flat sinus floors increased as RBH decreased. RBH was a significant predictor of failure with rates as follows: low- RBH = 5.1%, mid-RBH = 1.5%, and high-RBH = 0.4%. Flat sinus floors and single sites as compared to multiple sites had higher observed failure rates but neither achieved statistical significance; however, the power of the study was limited by low numbers of failures.CONCLUSION: The osteotome trifactorial classification system as proposed can assist planning OSFE cases and may allow better comparison of future OSFE studies.
|
['Adult', 'Dental Implantation, Endosseous', 'Dental Implants', 'Female', 'Follow-Up Studies', 'Humans', 'Male', 'Osteotomy', 'Outcome and Process Assessment, Health Care', 'Retrospective Studies', 'Sinus Floor Augmentation']
| 25,918,758
|
[['M01.060.116'], ['E04.545.550.280.280', 'E04.650.230.500', 'E06.645.550.280.280', 'E06.780.314.310'], ['D25.339.312', 'E06.780.346.593', 'E07.695.185', 'J01.637.051.339.312'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E04.555.580'], ['N04.761.559', 'N05.715.360.575'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E04.545.668', 'E04.555.130.550', 'E06.645.668']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Technology, Industry, and Agriculture [J]', 'Health Care [N]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 1
| 1
| 0
|
Alpha(1) adrenoceptor subtype selectivity. 3D-QSAR models for a new class of alpha(1) adrenoceptor antagonists derived from the novel antipsychotic sertindole.
|
Receptor-binding affinities for the alpha(1) adrenoceptor subtypes alpha(1a), alpha(1b) and alpha(1d) for a series of 39 alpha(1) adrenoceptor antagonists derived from the antipsychotic sertindole are reported. The SAR of the compounds with respect to affinity for the alpha(1a), alpha(1b) and alpha(1d) adrenoceptor subtypes as well as affinity obtained by an alpha(1) assay (rat brain membranes) were investigated using a 3D-QSAR approach based on the GRID/GOLPE methodology. Good statistics (r(2)=0.91-0.96; q(2)=0.65-0.73) were obtained with the combination of the water (OH2) and methyl (C3) probes. The combination of steric repulsion and electrostatic attractions explain the affinities of the included molecules. The adrenergic alpha(1a) receptor seems to be more tolerant to large substituents in the area between the indole 5- and 6-positions compared to the adrenergic alpha(1b) and alpha(1d) receptor subtypes. There seems to be minor differences in the position of areas in the alpha(1b) receptor compared to alpha(1a) and alpha(1d) receptors where electrostatic interaction between the molecules and the receptor (OH2 probe) contribute to increased affinity. These observations may be used in the design of new subtype selective compounds. In addition, the model based on biological data from an alpha(1) assay (rat brain membranes) resembles the model for the alpha(1b) adrenoceptor subtype.
|
['Adrenergic alpha-1 Receptor Antagonists', 'Drug Design', 'Imidazoles', 'Indoles', 'Models, Molecular', 'Quantitative Structure-Activity Relationship']
| 12,676,239
|
[['D27.505.519.625.050.200.100.100', 'D27.505.696.577.050.200.100.100'], ['E05.290.500', 'H01.158.703.007.338.500', 'H01.181.466.338.500'], ['D03.383.129.308'], ['D03.633.100.473'], ['E05.599.595'], ['G02.111.830.500', 'G07.690.773.997.500']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Phenomena and Processes [G]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
Essential and nonessential metal effects on extracellular Leishmania amazonensis in vitro.
|
Protozoan parasites like Leishmania amazonensis are excellent models to test the effects of new drugs against a functional molecular arsenal used to establish successfully an infection in the vertebrate host, where they invade the cells of the monocytic system. However, little is known about the influence of metal ions on the cellular functionality of the infective forms of L. amazonensis. In the present work, we show that ZnCl2 (an essential metal to cellular metabolism) did not induce drastic effects on the survival of the promastigote under the conditions tested. However, incubation of ZnCl2 prior to subsequent treatment with CdCl2 and HgCl2 led to a drastic toxic effect on parasite survival in vitro. Nonessential metals such as CdCl2 and HgCl2 promoted a drastic effect on parasite survival progressively with increasing dose and time of exposure. Notably, HgCl2 produced an effective elimination of the parasite in doses/time smaller than the CdCl2. This toxic action induced in the parasite a high condensation of the nuclear heterochromatin, besides the absence or de-structuring of functional organelles such as glycosomes, acidocalcisomes, and mitochondria in the cytoplasm. Our results suggest that promastigotes of L. amazonensis are sensitive to the toxic activity of nonessential metals, and that this activity increases when parasites are previously exposed to Zn. To summarize, toxic effects of the tested metals are dose and time dependent and can be used as a study model to better understand the functionality of the molecular arsenal responsible for the parasitism.
|
['Cadmium Chloride', 'Chlorides', 'Humans', 'Inhibitory Concentration 50', 'Leishmania mexicana', 'Mercuric Chloride', 'Microscopy, Electron, Transmission', 'Zinc Compounds']
| 31,881,207
|
[['D01.142.175', 'D01.210.450.150.125'], ['D01.210.450.150', 'D01.248.497.158.215'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.940.350', 'G07.690.936.563'], ['B01.268.475.868.488.410'], ['D01.210.450.150.525', 'D01.538.500'], ['E01.370.350.515.402.580', 'E05.595.402.580'], ['D01.975']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
A novel de novo 1.8 Mb microdeletion of 17q21.33 associated with intellectual disability and dysmorphic features.
|
We report on a de novo 17q21.33 microdeletion, 1.8 Mb in size, detected in a patient with mild intellectual disability, growth retardation, poor weight gain, microcephaly, long face, large beaked nose, thick lower lip, micrognathia and other dysmorphic features. The deletion was detected by whole-genome genotyping BeadChip assay and involves the genomic region between 45,682,246 and 47,544,816 bp on chromosome 17. Among the 24 RefSeq genes included in this deletion are the CA10 and CACNA1G genes that are involved in brain development and neurological processes. A possible candidate gene for the prenatal and postnatal growth retardation is the CHAD gene, which product chondroadherin is a cartilage protein with cell binding properties. These three genes may be responsible for the patient's phenotype.
|
['Abnormalities, Multiple', 'Adolescent', 'Calcium Channels, T-Type', 'Chromosome Deletion', 'Chromosomes, Human, Pair 17', 'Craniofacial Abnormalities', 'Extracellular Matrix Proteins', 'Facies', 'Humans', 'Intellectual Disability', 'Male', 'Microcephaly', 'Muscular Atrophy', 'Nerve Tissue Proteins', 'Smith-Magenis Syndrome', 'Syndrome']
| 22,842,074
|
[['C16.131.077'], ['M01.060.057'], ['D12.776.157.530.400.150.720', 'D12.776.543.550.450.150.720', 'D12.776.543.585.400.150.720'], ['C23.550.210.050.500.500', 'G05.365.590.029.530.175', 'G05.365.590.175.050.500.500', 'G05.365.590.762.180', 'G05.558.800.180', 'G05.700.131.500.500'], ['A11.284.187.520.300.415.425', 'G05.360.162.520.300.415.425'], ['C05.660.207', 'C16.131.621.207'], ['D12.776.860.300'], ['C23.550.291.812', 'E01.370.600.230'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C10.597.606.360', 'C23.888.592.604.646', 'F01.700.687', 'F03.625.539'], ['C05.660.207.620', 'C10.500.507.400.500', 'C16.131.621.207.620', 'C16.131.666.507.400.500'], ['C10.597.613.612', 'C23.300.070.500', 'C23.888.592.608.612'], ['D12.776.631'], ['C10.281.900', 'C16.131.077.879', 'C16.131.260.887', 'C16.320.180.887'], ['C23.550.288.500']]
|
['Diseases [C]', 'Named Groups [M]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Psychiatry and Psychology [F]']
| 1
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Effects of metoclopramide on the growth hormone response to galanin in normal man.
|
One of the most prominent metabolic effects of the systemic administration of the synthetic neuropeptide galanin in man is the increase in growth hormone (GH) secretion. This stimulating action of galanin is thought to occur directly at the hypothalamic level through the release of GHRH. Recently, it has been shown that also dopaminergic drugs may elicit GH secretion through an increase in hypothalamic GHRH secretion. The aim of this study was to investigate if the action of galanin on GHRH and consequently on GH release may be mediated via dopaminergic pathways evaluating the effects of a potent central dopaminergic receptor blocker, metoclopramide (MCP), on the galanin-induced growth hormone (GH) secretion in normal subjects. We studied seven young non obese healthy subjects (three females and four males). GH secretion was evaluated after 45 min iv infusion of porcine galanin (0.5 mg in 100 ml of saline) from 0 to 45 min combined with a 60 min iv infusion of a) saline (100 ml) or b) MCP (10 mg in 100 ml of saline) from -15 to 45 min. In all the seven subjects, during galanin infusion, GH values increased with respect to baseline with peaks occurring between 30 and 60 min after the beginning of galanin infusion. Peak GH values ranged between 3.5 and 15.4 micrograms/l (mean 10.4 +/- 1.6 micrograms/l). During MCP infusion no significant differences in the GH response to galanin with respect to saline were observed both when absolute GH levels and GH AUC were examined.(ABSTRACT TRUNCATED AT 250 WORDS)
|
['Adult', 'Animals', 'Dopamine', 'Female', 'Galanin', 'Growth Hormone', 'Humans', 'Immunoradiometric Assay', 'Male', 'Metoclopramide', 'Neuropeptides', 'Peptides', 'Swine']
| 7,693,566
|
[['M01.060.116'], ['B01.050'], ['D02.092.211.215.406', 'D02.092.311.342', 'D02.455.426.559.389.657.166.175.342'], ['D12.644.400.250', 'D12.776.631.650.250'], ['D06.472.699.631.525.425', 'D12.644.548.691.525.425'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.478.566.639.405', 'E05.601.470.639.405'], ['D02.065.277.573', 'D02.241.223.100.050.500.647', 'D02.241.223.100.100.510', 'D02.241.223.100.200.750', 'D02.241.223.100.300.350.625', 'D02.241.511.390.350.625', 'D02.455.426.559.389.127.020.937.647', 'D02.455.426.559.389.127.085.510', 'D02.455.426.559.389.127.250.750', 'D02.455.426.559.389.127.281.350.625', 'D02.455.426.559.389.657.654.638.625'], ['D12.644.400', 'D12.776.631.650'], ['D12.644'], ['B01.050.150.900.649.313.500.880']]
|
['Named Groups [M]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
A comparative study of the effect of the intrusion arch and straight wire mechanics on incisor root resorption: A randomized, controlled trial.
|
OBJECTIVE: To analyze and compare external apical root resorption (EARR) of maxillary incisors treated by intrusion arch or continuous archwire mechanics.MATERIALS AND METHODS: This cone-beam computed tomography (CBCT) study analyzed 28 deep bite patients in the permanent dentition who were randomly divided into two groups: Group 1, 12 patients with initial mean age of 15.1 ± 1.6 years and mean overbite of 4.6 ± 1.2 mm treated with the Connecticut intrusion arch (CIA) in the upper arch (Ortho Organizers, Carlsbad, Calif) for a mean period of 5.8 ± 1.27 months. Group 2, 16 patients with initial mean age of 22.1 ± 5.7 years and mean overbite of 4.1 ± 1.1 mm treated with conventional leveling and alignment using continuous archwire mechanics for 6.1 ± 0.81 months. The degree of EARR was detected in 112 maxillary incisors by using CBCT scans and a three-dimensional program (Dolphin 11.7, Dolphin Imaging & Management Solutions, Chatsworth, Calif). The CBCT scans were obtained before (T1) and 6 months after initiation of treatment (T2). Differences between and within groups were assessed by nonpaired and paired t-tests, respectively, with a 5% significance level.RESULTS: Significant differences were found for both groups between T1 and T2 ( P < .05) indicating that EARR occurred in both groups. However, there were no significant differences when EARR was compared between group 1 (-0.76 mm) and group 2 (-0.59 mm).CONCLUSIONS: The Connecticut intrusion arch did not lead to greater EARR of maxillary incisors when compared with conventional orthodontic mechanics.
|
['Adolescent', 'Biomechanical Phenomena', 'Cone-Beam Computed Tomography', 'Female', 'Humans', 'Incisor', 'Male', 'Maxilla', 'Overbite', 'Prospective Studies', 'Radiographic Image Interpretation, Computer-Assisted', 'Root Resorption', 'Software', 'Tooth Movement Techniques', 'Treatment Outcome', 'Young Adult']
| 28,985,106
|
[['M01.060.057'], ['G01.154.090', 'G01.374.089'], ['E01.370.350.700.810.810.490', 'E01.370.350.825.810.810.399'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A14.549.167.860.425'], ['A02.835.232.781.324.502.645', 'A14.521.645'], ['C07.793.494.630.500'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['E01.158.600.680', 'E01.370.350.350.700', 'E01.370.350.700.705', 'L01.313.500.750.100.158.600.680'], ['C07.793.901.653', 'G10.549.855.653'], ['L01.224.900'], ['E06.658.578.836'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Anatomy [A]', 'Diseases [C]', 'Health Care [N]', 'Information Science [L]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 1
| 1
| 0
|
Mutational alterations of translational coupling in the L11 ribosomal protein operon of Escherichia coli.
|
The L11 operon in Escherichia coli consists of the genes coding for ribosomal proteins L11 and L1. It is known that translation of L1 does not take place unless the preceding L11 cistron is translated, that is, the two cistrons are translationally coupled, and this is the basis of coregulation of the translation of the two cistrons by a single repressor, L1. Several mutational analyses were carried out to define the region responsible for coupling L1 translation with L11 translation. First, by introducing several amber mutations into the L11 gene by a site-directed mutagenesis technique, it was shown that translation by ribosomes down to a position 21 nucleotides upstream, but not to a position 45 nucleotides upstream, from the end of the L11 cistron allowed the initiation of L11 translation. Second, deletion analysis indicated that a region located 23 to 20 nucleotides from the end of the L11 gene was involved in preventing independent initiation from L1 translation. Third, five different mutations obtained by screening for activation of the masked L1 initiation site were found to be clustered in a small region immediately upstream from the Shine-Dalgarno sequence of L1, and all of them were G-to-A transitions. These results, together with some additional experiments with oligonucleotide-directed mutagenesis, defined the region involved in the coupling and suggest that some special feature of this region, probably different from simple masking of the initiation site by base pairing, is responsible for translational coupling. The present results also suggest that there might be specific differences in the primary nucleotide sequence that distinguish independent translational initiation sites from translationally coupled (i.e., masked) initiation sites.
|
['Bacterial Proteins', 'Chromosome Deletion', 'Escherichia coli', 'Mutation', 'Operon', 'Plasmids', 'Protein Biosynthesis', 'Ribosomal Proteins']
| 3,301,803
|
[['D12.776.097'], ['C23.550.210.050.500.500', 'G05.365.590.029.530.175', 'G05.365.590.175.050.500.500', 'G05.365.590.762.180', 'G05.558.800.180', 'G05.700.131.500.500'], ['B03.440.450.425.325.300', 'B03.660.250.150.180.100'], ['G05.365.590'], ['G05.360.340.024.686', 'G05.360.340.358.207.500'], ['G05.360.600'], ['G02.111.660.871', 'G03.734.871', 'G05.297.670'], ['D12.776.835']]
|
['Chemicals and Drugs [D]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Workplace bullying in health care affects the meaning of work.
|
Our purpose in this grounded theory study was to explore the impact of workplace bullying (WPB) on women working in health care. We analyzed interviews with 21 women, professionals and nonprofessionals. The women experienced a change in their meaning of work (MOW) when they had experienced WPB, and they addressed this change through a process we called the shifting meaning of work. This process has three stages. The first, developing insight, involves recognizing causes of changed MOW as external. In the second stage, resisting, women defend against changed MOW by sustaining acceptable MOW and work performances, and by confronting causes. In the final stage, rebuilding, women try to adapt and modify approaches to work by coming to terms, adjusting work attitudes, and investing in self. We identified implications of this process for managing health and work issues with women, health care providers, and employers.
|
['Adaptation, Psychological', 'Adult', 'Aggression', 'Female', 'Health Personnel', 'Humans', 'Job Satisfaction', 'Knowledge', 'Middle Aged', 'Philosophy', 'Stress, Psychological', 'Violence', "Women's Health", 'Young Adult']
| 20,463,362
|
[['F01.058'], ['M01.060.116'], ['F01.145.126.125', 'F01.145.813.045'], ['M01.526.485', 'N02.360'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F02.784.692.425'], ['K01.468'], ['M01.060.116.630'], ['K01.752'], ['F01.145.126.990', 'F02.830.900'], ['I01.198.240.856', 'I01.880.735.900'], ['N01.400.900'], ['M01.060.116.815']]
|
['Psychiatry and Psychology [F]', 'Named Groups [M]', 'Health Care [N]', 'Organisms [B]', 'Humanities [K]', 'Anthropology, Education, Sociology, and Social Phenomena [I]']
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 0
|
Advantages of sentence-level fMRI language tasks in presurgical language mapping for temporal lobe epilepsy.
|
fMRI language mapping has become increasingly utilized for determining language dominance before surgical intervention for temporal lobe epilepsy (TLE). This study aimed to examine the differences between two classes of fMRI word generation tasks used in our clinic: tasks using a single word cue, referred to as simple generative tasks (SGTs), and tasks also involving sentence-level processing, referred to as sentence-level language tasks (SLTs). Specifically, we aimed to investigate the extent and laterality of activation and frontal-temporal connectivity during these language tasks and their relationship to clinical language measures. Thirty-one patients with TLE (18 patients with left TLE and 13 patients with right TLE) performed four language tasks during an fMRI scan, two SGTs and two SLTs. We found significantly greater activity for SLTs over SGTs in bilateral inferior frontal and middle temporal gyri and the left temporal pole. Sentence-level language tasks also showed greater lateralization compared with SGTs. Finally, we found that while activation extent did not correlate with clinical language tests, the degree of left frontal-temporal connectivity was significantly correlated with naming and semantic fluency performance. These correlations also were more robust for SLTs than for SGTs. Taken together, these results provide a compelling argument for including some form of SLTs in fMRI language lateralization protocols for TLE as they allow for better characterization of language networks, particularly in the temporal lobes which are at risk in surgery.
|
['Adult', 'Brain Mapping', 'Epilepsy', 'Epilepsy, Temporal Lobe', 'Female', 'Functional Laterality', 'Humans', 'Language', 'Language Tests', 'Magnetic Resonance Imaging', 'Male', 'Middle Aged', 'Neuropsychological Tests', 'Temporal Lobe']
| 24,534,479
|
[['M01.060.116'], ['E01.370.350.578.875.500', 'E01.370.376.537.625.500', 'E05.629.875.500'], ['C10.228.140.490'], ['C10.228.140.490.360.290', 'C10.228.140.490.493.375'], ['F02.830.297.425', 'G11.561.225.425'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F01.145.209.399', 'L01.559'], ['F04.711.513.240'], ['E01.370.350.825.500'], ['M01.060.116.630'], ['F04.711.513'], ['A08.186.211.200.885.287.500.863']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Information Science [L]', 'Anatomy [A]']
| 1
| 1
| 1
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 1
| 1
| 0
| 0
|
Combined effects of caffeine and alcohol on hemodynamics and coronary artery blood flow in dogs.
|
Alcohol intake is often followed by coffee drinking because of the universal acceptance of its sobering effect. Such effects were found inconsistent on motor functions. However, it is common belief that caffeine will antagonize the intoxicating effects of alcohol. The independent actions of caffeine and alcohol are well documented, but combined effects of short-term administration are unknown. This experimental work was designed to study the effects due to short-term administration of caffeine and alcohol on the cardiovascular system. In phase I, 30 experiments were performed in our laboratories to study the dose-response curves of both the drugs. In phases II and III, 15 dogs were subjected to 30 experiments. In phase II, caffeine, 5 mg/kg, was given i.v., followed by ethanol, 400 mg/kg i.v., and in phase III, sequence of drug administration was reversed to study the effects on hemodynamics and coronary artery blood flow. Caffeine did not show significant changes in all the cardiovascular parameters, and ethanol administration caused nonsignificant increase in heart rate, mean arterial pressure, left ventricular systolic pressure, and left ventricular (LV) mechanical work and decrease in the maximal rate of first derivative of LV pressure, stroke volume, and systemic vascular resistance. Left ventricular end-diastolic, pulmonary artery mean and right atrial pressures, pulmonary vascular resistance, myocardial oxygen consumption, and as coronary flow reserve increased as compared with controls. Combined caffeine and alcohol had synergistic effects, but when the order of drug administration was reversed (i.e., alcohol was followed by caffeine), the effect was antagonistic.
|
['Animals', 'Arrhythmias, Cardiac', 'Arteries', 'Blood Flow Velocity', 'Caffeine', 'Central Nervous System Depressants', 'Central Nervous System Stimulants', 'Coronary Circulation', 'Dogs', 'Drug Administration Schedule', 'Drug Interactions', 'Ethanol', 'Hemodynamics']
| 9,890,396
|
[['B01.050'], ['C14.280.067', 'C23.550.073'], ['A07.015.114'], ['E01.370.370.130', 'G09.330.380.630.080'], ['D03.132.960.175', 'D03.633.100.759.758.824.175'], ['D27.505.696.277', 'D27.505.954.427.210'], ['D27.505.696.282', 'D27.505.954.427.220'], ['G09.330.100.324'], ['B01.050.150.900.649.313.750.250.216.200'], ['E02.319.283'], ['G07.690.773.968'], ['D02.033.375'], ['G09.330.380']]
|
['Organisms [B]', 'Diseases [C]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Integrated microHall magnetometer to measure the magnetic properties of nanoparticles.
|
Magnetic nanoparticles (MNPs) are widely used in biomedical and clinical applications, including medical imaging, therapeutics, and biological sample processing. Rapid characterization of MNPs, notably their magnetic moments, should facilitate optimization of particle synthesis and accelerate assay development. Here, we report a compact and low-cost magnetometer for fast, on-site MNP characterization. Termed integrated microHall magnetometer (iHM), our device was fabricated using standard semiconductor processes: an array of Hall sensors, transistor switches, and amplifiers were integrated into a single chip, thus improving the detection sensitivity and facilitating chip operation. By applying the iHM, we demonstrate versatile magnetic assays. We measured the magnetic susceptibility and moments of MNPs using small sample amounts (?10 pL), identified different MNP compositions in mixtures, and detected MNP-labeled single cells.
|
['Cell Line, Tumor', 'Equipment Design', 'Humans', 'Lab-On-A-Chip Devices', 'Magnetite Nanoparticles', 'Magnetometry', 'Metals']
| 29,067,383
|
[['A11.251.210.190', 'A11.251.860.180'], ['E05.320'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E07.305.343.500'], ['J01.637.512.600.500.144.500'], ['E05.540'], ['D01.552']]
|
['Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Technology, Industry, and Agriculture [J]', 'Chemicals and Drugs [D]']
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
The tumor suppressor CYLD interacts with TRIP and regulates negatively nuclear factor kappaB activation by tumor necrosis factor.
|
Cylindromas are benign adnexal skin tumors caused by germline mutations in the CYLD gene. In most cases the second wild-type allele is lost in tumor tissue, suggesting that CYLD functions as tumor suppressor. CYLD is a protein of 956 amino acids harboring a functional deubiquitinating domain at the COOH-terminal end. To shed more light on the function of CYLD, we have performed a yeast two hybrid screen using an HaCaT cDNA library that identified the RING finger protein TRIP (TRAF-interacting protein) as interactor with full-length CYLD. Mapping of the interacting domains revealed that the central domain of CYLD binds to the COOH-terminal end of TRIP. Far Western analysis and coimmunoprecipitations in mammalian cells confirmed that full-length CYLD binds to the COOH-terminal domain of TRIP. Because TRIP is an inhibitor of nuclear factor (NF)-kappaB activation by tumor necrosis factor (TNF), the effect of CYLD on NF-kappaB activation was investigated in HeLa cells. The results established that CYLD down-regulates NF-kappaB activation by TNF-alpha. The inhibition by CYLD depends on the presence of the central domain interacting with TRIP and its deubiquitinating activity. These findings indicate that cylindromas arise through constitutive NF-kappaB activation leading to hyperproliferation and tumor growth.
|
['Animals', 'COS Cells', 'Deubiquitinating Enzyme CYLD', 'HeLa Cells', 'Humans', 'NF-kappa B', 'RNA-Binding Proteins', 'Receptors, Tumor Necrosis Factor', 'Signal Transduction', 'Tumor Necrosis Factor Receptor-Associated Peptides and Proteins', 'Tumor Necrosis Factor-alpha', 'Tumor Suppressor Proteins']
| 14,676,304
|
[['B01.050'], ['A11.251.210.172.500', 'A11.329.228.220'], ['D08.811.037.375', 'D12.776.624.776.482'], ['A11.251.210.190.400', 'A11.251.860.180.400', 'A11.436.340'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D05.500.672', 'D12.776.260.600', 'D12.776.660.600', 'D12.776.930.600'], ['D12.776.157.725', 'D12.776.664.962'], ['D12.776.543.750.705.852.760'], ['G02.111.820', 'G04.835'], ['D12.644.360.024.500', 'D12.776.157.057.500', 'D12.776.476.024.500'], ['D12.644.276.374.500.800', 'D12.644.276.374.750.626', 'D12.776.124.900', 'D12.776.395.930', 'D12.776.467.374.500.800', 'D12.776.467.374.750.626', 'D23.529.374.500.800', 'D23.529.374.750.626'], ['D12.776.624.776']]
|
['Organisms [B]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Thyroid stimulating hormone assay as the first line biochemical parameter to determine thyroid gland abnormalities.
|
Increased cellular catabolic activities observed in hyperthyroid state had been established. This is consequent to excessive hormones secreted by the thyroid gland during this condition. A total of 60 subjects comprising of 45 females mean age 43.02 +/- 1.90 (range 22-70 years) and 15 males mean age 50.40 +/- 3.59 (range 25-68) and 60 controls comprising of 45 females mean age 41.18 +/- 1.68 (range 22-68 years) and 15 males mean age 40.53 +/- 2.88 (range 25-65) were recruited for the study. The plasma level of T4, T3 and TSH were determined in both the experiment group and the controls. A significant increase (p < 0.05) in plasma T4, T3 and a significant decrease (p < 0.05) in plasma TSH were observed in the experiment group in comparison to the controls. Also, an inverse relationship was noted to exist between the plasma T4 and T3; and TSH in primary hyperthyroid state.
|
['Adult', 'Aged', 'Blood Chemical Analysis', 'Female', 'Humans', 'Hyperthyroidism', 'Male', 'Middle Aged', 'Sex Factors', 'Thyrotropin', 'Young Adult']
| 24,783,789
|
[['M01.060.116'], ['M01.060.116.100'], ['E01.370.225.124.100', 'E05.200.124.100'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C19.874.397'], ['M01.060.116.630'], ['N05.715.350.675', 'N06.850.490.875'], ['D06.472.699.631.525.883', 'D12.644.548.691.525.883'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Diseases [C]', 'Health Care [N]', 'Chemicals and Drugs [D]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Kinematics after tear in the anterior cruciate ligament: dynamic bilateral radiostereometric studies in 11 patients.
|
We studied the kinematics of both knees using radiostereometry in 11 patients with unilateral injury of the anterior cruciate ligament and normal contralateral knee. Continuous radiostereometric exposures at a speed of 24 exposures a second were performed, when the patients ascended an 8 cm high platform. The tibial center was more dorsally displaced and the tibia more externally rotated on the injured side. This increasing external tibial rotation was associated with increased anterior displacement of the lateral femoral condyle. The latter also displayed less anterior-posterior translations during continuous extension. The anterior-posterior translation of the medial condyle was about the same as on the uninjured side. Changes in the kinematics of the knee joint due to rupture of the anterior cruciate ligament can result in an abnormal joint load, which may increase the risk of damage to the cartilage and the menisci.
|
['Adolescent', 'Adult', 'Anterior Cruciate Ligament', 'Anterior Cruciate Ligament Injuries', 'Chronic Disease', 'Female', 'Femur', 'Humans', 'Joint Instability', 'Knee Joint', 'Male', 'Photogrammetry', 'Radiography', 'Range of Motion, Articular', 'Risk Factors', 'Rotation', 'Rupture', 'Tibia', 'Weight-Bearing']
| 11,580,126
|
[['M01.060.057'], ['M01.060.116'], ['A02.513.514.100', 'A02.835.583.512.100', 'A10.165.669.514.100'], ['C26.558.554.213'], ['C23.550.291.500'], ['A02.835.232.043.150'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C05.550.521'], ['A02.835.583.475'], ['E01.370.350.600.630'], ['E01.370.350.700'], ['E01.370.600.700', 'G11.427.760'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['G01.482.703'], ['C26.761'], ['A02.835.232.043.650.883'], ['G01.374.965']]
|
['Named Groups [M]', 'Anatomy [A]', 'Diseases [C]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
[Low-dose CT perfusion imaging based on pre-scan regulation and on reconstruction with sparsity constraints].
|
The long-period CT perfusion imaging leads to an excess amount of radiation dose to the patient. However, the radiation dose could be significantly reduced if a previous normal-dose image is acquired before a set of low-dose scans of perfusion, and a filtering processing is performed on the differences between the current low-dose images and the previous normal-dose image, then the results are added to the previous image. But the selection of plenty of parameters makes the algorithm complicated. This paper proposes an innovative approach performed in sinogram domain instead of in image domain. First a normal-dose image and a set of low-dose projection data are acquired before the perfusion. Second the perfusion information is commendably reconstructed with sparsity constraints of the differences between current low-dose perfusion sinograms and previous low-dose sinogram. Finally, the reconstructed perfusion information is added to the previous normal-dose image. The proposed method was validated by simulated experiments with a set of brain CT perfusion images, which showed that the new method provided more accurate perfusion information; the time-attenuation curve was more close to that for normal-dose scan and the mean transit time more repeatable.
|
['Algorithms', 'Brain', 'Humans', 'Perfusion Imaging', 'Radiation Dosage', 'Radiation Injuries', 'Radiographic Image Interpretation, Computer-Assisted', 'Subtraction Technique', 'Tomography, X-Ray Computed']
| 22,403,999
|
[['G17.035', 'L01.224.050'], ['A08.186.211'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.350.710.600', 'E01.370.384.730.354'], ['E05.799.513', 'G01.750.740', 'N06.850.810.250'], ['C26.733', 'G01.750.748.500', 'N06.850.460.350.850.500', 'N06.850.810.300.360'], ['E01.158.600.680', 'E01.370.350.350.700', 'E01.370.350.700.705', 'L01.313.500.750.100.158.600.680'], ['E01.370.350.760'], ['E01.370.350.350.810', 'E01.370.350.600.350.700.810', 'E01.370.350.700.700.810', 'E01.370.350.700.810.810', 'E01.370.350.825.810.810']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Anatomy [A]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Diseases [C]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 1
| 0
|
In-gel microwave-assisted acid hydrolysis of proteins combined with liquid chromatography tandem mass spectrometry for mapping protein sequences.
|
We report an enabling method for mapping the protein sequence with high sequence coverage. This method combines the high separation power of gel electrophoresis for protein separation with the high sequence coverage capability of microwave-assisted acid hydrolysis (MAAH) mass spectrometry (MS). In-gel MAAH using 25% trifluoroacetic acid was developed and optimized for degrading the gel-separated protein into small peptides suitable for tandem MS sequencing. For bovine serum albumin (BSA) (?67 kDa), with 4 ìg of protein loading onto a gel for separation, followed by excising the protein gel band for in-gel MAAH and then injecting ?2 ìg of the resultant peptides into a liquid chromatography quadrupole time-of-flight mass spectrometer for analysis, 689 ± 54 (n = 3) unique peptides were identified with a protein sequence coverage of 99 ± 1%. Both the number of peptides detected and sequence coverage decreased as the sample amount decreased, mainly due to background interference: 316 ± 59 peptides and 94 ± 3% coverage for 2 ìg loading, 136 ± 19 and 76 ± 5% for 1 ìg loading, and 30 ± 2 and 32 ± 2% for 0.5 ìg loading. To demonstrate the general applicability of the method, 10 gel bands from gel electrophoresis of an albumin-depleted human plasma sample were excised for in-gel MAAH LC-MS analysis. In total, 19 relatively high abundance proteins with molecular weights ranging from ?8 to ?160 kD could be mapped with coverage of 100% for six proteins (MW 8759 to 68 425 Da), 96-98% for five proteins (MW 11 458 to 36 431 Da), 92% for three proteins (MW 15 971 to 36 431 Da), 80-87% for four proteins (MW 42 287 to 162 134 Da), and 56% for one protein (MW 51 358 Da). Finally, to demonstrate the applicability of the method for more detailed analysis of complex protein mixtures, two-dimensional (2D) gel electrophoresis was combined with in-gel MAAH, affinity purification, and LC-MS/MS to characterize six bovine alpha-S1-casein phosphoprotein isoforms. Full sequence coverage was achieved for each protein, and six new modification sites were found.
|
['Animals', 'Caseins', 'Cattle', 'Chromatography, Liquid', 'Chromosome Mapping', 'Electrophoresis, Gel, Two-Dimensional', 'Humans', 'Hydrolysis', 'Microwaves', 'Serum Albumin', 'Tandem Mass Spectrometry']
| 24,308,638
|
[['B01.050'], ['D12.776.256.159.750.207', 'D12.776.744.150'], ['B01.050.150.900.649.313.500.380.271'], ['E05.196.181.400'], ['E05.393.183'], ['E05.196.401.250', 'E05.301.300.230'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G02.380'], ['G01.358.500.505.810.500', 'G01.750.250.810.500', 'G01.750.770.721.500'], ['D12.776.034.841', 'D12.776.124.727'], ['E05.196.566.880']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Characterization of Pseudomonas stutzeri NT-I capable of removing soluble selenium from the aqueous phase under aerobic conditions.
|
Pseudomonas stutzeri strain NT-I was isolated from the drainage wastewater of a selenium refinery plant. This bacterium efficiently reduced selenate to elemental selenium without prolonged accumulation of selenite under aerobic conditions. Strain NT-I was able to reduce selenate completely at high concentrations (up to 10 mM) and selenite almost completely (up to 9 mM). In addition, higher concentrations of selenate and selenite were substantially reduced. Activity was observed under the following experimental conditions: 20-50°C, pH 7-9, and 0.05-20 g L(-1) NaCl for selenate reduction, and 20-50°C, pH 6-9, and 0.05-50 g L(-1) NaCl for selenite reduction. Under anaerobic conditions, selenate was reduced more rapidly, whereas selenite was not reduced at all. The high selenate- and selenite-reducing capability at high concentrations suggested that strain NT-I is suitable for the removal of selenium from high-strength industrial wastewater.
|
['Industrial Waste', 'Oxidation-Reduction', 'Oxidoreductases', 'Pseudomonas stutzeri', 'Selenium', 'Water Microbiology']
| 21,676,651
|
[['D20.944.420', 'N06.850.460.710.420'], ['G02.700', 'G03.295.531'], ['D08.811.682'], ['B03.440.400.425.625.625.750', 'B03.660.250.580.590.750'], ['D01.268.185.850', 'D01.578.700'], ['H01.158.273.540.274.777', 'N06.850.425.450']]
|
['Chemicals and Drugs [D]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Disciplines and Occupations [H]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
|
Amphiphilic pyridinium salts block TNF alpha/NF kappa B signaling and constitutive hypersecretion of interleukin-8 (IL-8) from cystic fibrosis lung epithelial cells.
|
Cystic fibrosis (CF) is a common, lethal genetic disease, which is due to mutations in the CFTR gene. The CF lung expresses a profoundly proinflammatory phenotype, due to constitutive hypersecretion of IL-8 from epithelial cells lining the airways. In a systematic search for candidate drugs that might be used therapeutically to suppress IL-8 secretion from these cells, we have identified a potent and efficacious series of amphiphilic pyridinium salts. The most potent of these salts is MRS2481, an (R)-1-phenylpropionic acid ester, with an IC50 of ca. 1microM. We have synthesized 21 analogues of MRS2481, which have proven sufficient to develop a preliminary structure-activity relationship (SAR). For optimal activity, we have found that the ester must be connected to the pyridinium derivative by an eight-carbon chain. An optical isomer of the lead compound, containing an (S)-1-phenylpropionic acid ester, has been found to be a much less active. The mechanism of action of MRS2481 appears to involve inhibition of signaling of the NF(kappa)B and AP-1 transcription factors to the IL-8 promoter. MRS2481 is a potent inhibitor of TNFalpha-induced phosphorylation and proteosomal destruction of I(kappa)B(alpha). Inasmuch as I(kappa)B(alpha) is the principal inhibitor of the NF(kappa)B signaling pathway, preservation of intact I(kappa)B(alpha) would serve to keep the IL-8 promoter silent. We also find that MRS2481 blocks TNF(alpha)-activated phosphorylation of JNK, the c-JUN kinase. The IL-8 promoter is also activated by an AP-1 site, which requires a phospho-c-JUN/c-FOS dimer for activity. We therefore interpret these data to suggest that the mechanism of MRS2481 action is to inhibit both NF(kappa)B and AP-1 signaling on the IL-8 promoter. Given the medicinally promising properties of water-solubility, potency in the low muM concentration range, and high efficacy, we anticipate that MRS2481, or a further optimized derivative, may find an important place in the armamentarium of pharmaceutical strategies yet to be arrayed against the inflammatory phenotype of the CF lung.
|
['Cystic Fibrosis', 'Dose-Response Relationship, Drug', 'HeLa Cells', 'Humans', 'Interleukin-8', 'NF-kappa B', 'Pyridinium Compounds', 'Respiratory Mucosa', 'Salts', 'Signal Transduction', 'Surface-Active Agents', 'Tumor Necrosis Factor-alpha']
| 15,963,954
|
[['C06.689.202', 'C08.381.187', 'C16.320.190', 'C16.614.213'], ['G07.690.773.875', 'G07.690.936.500'], ['A11.251.210.190.400', 'A11.251.860.180.400', 'A11.436.340'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.644.276.374.200.120.800', 'D12.644.276.374.465.312', 'D12.776.467.374.200.120.800', 'D12.776.467.374.465.246', 'D23.125.300.120.800', 'D23.469.200.120.800', 'D23.529.374.200.120.800', 'D23.529.374.465.312'], ['D05.500.672', 'D12.776.260.600', 'D12.776.660.600', 'D12.776.930.600'], ['D03.383.725.762'], ['A04.760', 'A10.615.550.760'], ['D01.786'], ['G02.111.820', 'G04.835'], ['D27.720.877'], ['D12.644.276.374.500.800', 'D12.644.276.374.750.626', 'D12.776.124.900', 'D12.776.395.930', 'D12.776.467.374.500.800', 'D12.776.467.374.750.626', 'D23.529.374.500.800', 'D23.529.374.750.626']]
|
['Diseases [C]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Organisms [B]', 'Chemicals and Drugs [D]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Emergency management of hypercyanotic crises in tetralogy of Fallot.
|
We report the case of a 3-month-old girl with tetralogy of Fallot who was resuscitated from a near-fatal hypercyanotic episode (Tet spell). While tetralogy of Fallot is rare (1 in 10,000 live births), failures of diagnosis or management may result in catastrophic outcomes for an eminently survivable condition. An understanding of the pathophysiology of hypercyanotic spells will allow the emergency specialist to understand and apply a treatment option--compression of the abdominal aorta--not previously reported in the emergency medicine literature.
|
['Aorta, Abdominal', 'Constriction', 'Cyanosis', 'Emergencies', 'Female', 'Humans', 'Infant', 'Oxygen', 'Tetralogy of Fallot']
| 7,832,359
|
[['A07.015.114.056.205'], ['E05.225'], ['C23.888.248'], ['C23.550.291.781', 'N06.230.100.083', 'N06.850.376'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703'], ['D01.268.185.550', 'D01.362.670'], ['C14.240.400.849', 'C14.280.400.849', 'C16.131.240.400.849']]
|
['Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Health Care [N]', 'Organisms [B]', 'Named Groups [M]', 'Chemicals and Drugs [D]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Development of a singleplex PCR assay for rapid identification and differentiation of Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, Cryptococcus gattii, and hybrids.
|
A singleplex PCR assay using a single primer pair targeting the putative sugar transporter gene was developed here to distinguish Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, and Cryptococcus gattii according to the distinct size of the amplicon. The interspecies and intravarietal hybrids were also characterized on the basis of distinct combined profiles of amplicons. This PCR assay is a rapid, simple, and reliable approach suitable for laboratory diagnoses and large-scale epidemiologic studies.
|
['Cryptococcosis', 'Cryptococcus gattii', 'Cryptococcus neoformans', 'DNA Primers', 'DNA, Fungal', 'Humans', 'Microbiological Techniques', 'Molecular Diagnostic Techniques', 'Molecular Sequence Data', 'Polymerase Chain Reaction', 'Recombination, Genetic', 'Sequence Analysis, DNA', 'Time Factors']
| 23,536,400
|
[['C01.150.703.248'], ['B01.300.381.258.300', 'B01.300.930.316.300'], ['B01.300.381.258.366', 'B01.300.930.316.366'], ['D13.695.578.424.450.275', 'D27.720.470.530.600.223.600'], ['D13.444.308.300'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.225.875', 'E05.200.875'], ['E01.370.225.880', 'E05.200.880', 'E05.393.520'], ['L01.453.245.667'], ['E05.393.620.500'], ['G05.728'], ['E05.393.760.700'], ['G01.910.857']]
|
['Diseases [C]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Information Science [L]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Fatal neuroleptic malignant syndrome in a previously long-term user of clozapine following its reintroduction in combination with paroxetine.
|
A 77-year-old patient with initial behavioral and psychological symptoms of dementia was treated with clozapine (50 mg/daily). Since no clinical benefit was apparent, clozapine was discontinued after six weeks and the patient started on paroxetine (20 mg/daily). After three weeks on paroxetine, he was given another trial of clozapine at a starting dosage of 25 mg/daily. While clozapine had previously been well tolerated, this time he rapidly developed fever, mental confusion, lethargy, muscle spasms and rigidity. The diagnosis of neuroleptic malignant syndrome was delayed, because there was no leukocytosis and serum creatine phosphokinase was initially not elevated. Subcutaneous apomorphine was then given but, after an initial improvement, the patient developed a multiple organ failure syndrome and died.
|
['Aged', 'Antipsychotic Agents', 'Clozapine', 'Drug Therapy, Combination', 'Fatal Outcome', 'Humans', 'Male', 'Neuroleptic Malignant Syndrome', 'Paroxetine', 'Serotonin Uptake Inhibitors']
| 16,804,375
|
[['M01.060.116.100'], ['D27.505.696.277.950.040', 'D27.505.954.427.210.950.040', 'D27.505.954.427.700.872.331'], ['D03.633.300.240.220'], ['E02.319.310'], ['E05.318.308.985.550.325', 'N01.224.935.698.201', 'N06.850.505.400.975.550.325', 'N06.850.520.308.985.550.325'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C10.228.140.079.737', 'C10.720.737', 'C25.723.705.600'], ['D03.383.621.600'], ['D27.505.519.562.437.850', 'D27.505.519.625.600.850', 'D27.505.519.625.850.900', 'D27.505.696.577.600.850', 'D27.505.696.577.850.900']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Phase II study of cediranib, an oral pan-vascular endothelial growth factor receptor tyrosine kinase inhibitor, in patients with recurrent glioblastoma.
|
PURPOSE: Glioblastoma is an incurable solid tumor characterized by increased expression of vascular endothelial growth factor (VEGF). We performed a phase II study of cediranib in patients with recurrent glioblastoma.METHODS: Cediranib, an oral pan-VEGF receptor tyrosine kinase inhibitor, was administered (45 mg/d) until progression or unacceptable toxicity to patients with recurrent glioblastoma. The primary end point was the proportion of patients alive and progression free at 6 months (APF6). We performed magnetic resonance imaging (MRI) and plasma and urinary biomarker evaluations at multiple time points.RESULTS: Thirty-one patients with recurrent glioblastoma were accrued. APF6 after cediranib was 25.8%. Radiographic partial responses were observed by MRI in 17 (56.7%) of 30 evaluable patients using three-dimensional measurements and in eight (27%) of 30 evaluable patients using two-dimensional measurements. For the 15 patients who entered the study taking corticosteroids, the dose was reduced (n = 10) or discontinued (n = 5). Toxicities were manageable. Grade 3/4 toxicities included hypertension (four of 31; 12.9%); diarrhea (two of 31; 6.4%); and fatigue (six of 31; 19.4%). Fifteen (48.4%) of 31 patients required at least one dose reduction and 15 patients required temporary drug interruptions due to toxicity. Drug interruptions were not associated with outcome. Changes in plasma placental growth factor, basic fibroblast growth factor, matrix metalloproteinase (MMP) -2, soluble VEGF receptor 1, stromal cell-derived factor-1alpha, and soluble Tek/Tie2 receptor and in urinary MMP-9/neutrophil gelatinase-associated lipocalin activity after cediranib were associated with radiographic response or survival.CONCLUSION: Cediranib monotherapy for recurrent glioblastoma is associated with encouraging proportions of radiographic response, 6-month progression-free survival, and a steroid-sparing effect with manageable toxicity. We identified early changes in circulating molecules as potential biomarkers of response to cediranib. The efficacy of cediranib and the predictive value of these candidate biomarkers will be explored in prospective trials.
|
['Adult', 'Aged', 'Antineoplastic Agents', 'Biomarkers, Tumor', 'Brain Neoplasms', 'Disease-Free Survival', 'Female', 'Glioblastoma', 'Humans', 'Male', 'Middle Aged', 'Neoplasm Recurrence, Local', 'Protein Kinase Inhibitors', 'Quinazolines', 'Receptors, Vascular Endothelial Growth Factor', 'Young Adult']
| 20,458,050
|
[['M01.060.116'], ['M01.060.116.100'], ['D27.505.954.248'], ['D23.101.140'], ['C04.588.614.250.195', 'C10.228.140.211', 'C10.551.240.250'], ['E01.789.800.190', 'E05.318.740.998.300', 'N04.761.559.590.800.190', 'N05.715.360.575.575.800.190', 'N05.715.360.750.795.300', 'N06.850.520.830.998.300'], ['C04.557.465.625.600.380.080.335', 'C04.557.470.670.380.080.335', 'C04.557.580.625.600.380.080.335'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['C04.697.655', 'C23.550.727.655'], ['D27.505.519.389.755'], ['D03.633.100.786'], ['D08.811.913.696.620.682.725.400.950', 'D12.776.543.750.630.750', 'D12.776.543.750.750.400.910'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Double loss-of-function mutation in EARLY FLOWERING 3 and CRYPTOCHROME 2 genes delays flowering under continuous light but accelerates it under long days and short days: an important role for Arabidopsis CRY2 to accelerate flowering time in continuous light.
|
The photoperiodic response is one of the adaptation mechanisms to seasonal changes of lengths of day and night. The circadian clock plays pivotal roles in this process. In Arabidopsis, LHY, CCA1, ELF3, and other clock proteins play major roles in maintaining circadian rhythms. lhy;cca1 double mutants with severe defects in circadian rhythms showed accelerated flowering under short days (SDs), but delayed flowering under continuous light (LL). The protein level of the floral repressor SVP increased in lhy;cca1 mutants under LL, and the late-flowering phenotype of lhy;cca1 mutants was partially suppressed by svp, flc, or elf3. ELF3 interacted with both CCA1 and SVP, and elf3 suppressed the SVP accumulation in lhy;cca1 under LL. These results suggest that the unique mechanism of the inversion of the flowering response of lhy;cca1 under LL may involve both the ELF3-SVP/FLC-dependent and -independent pathways. In this work, elf3-1 seeds were mutagenized with heavy-ion beams and used to identify mutation(s) that delayed flowering under LL but not long days (LDs) or SDs even without ELF3. In this screening, seven candidate lines named suppressor of elf3 1 (self1), sel3, sel5, sel7, sel14, sel15, and sel20 were identified. Genetic analysis indicated that sel20 was a new deletion allele of a mutation in the blue light receptor, CRY2. A late-flowering phenotype and decrease of FT expression in the elf3;sel20 double mutant was obvious under LL but not under SDs or LDs. These results indicated that the late-flowering phenotype in the double mutant elf3;sel20 as well as in lhy;cca1 was affected by the presence of darkness. The results suggest that CRY2 may play more essential roles in the acceleration of flowering under LL than LDs or SDs.
|
['Alleles', 'Arabidopsis', 'Arabidopsis Proteins', 'Cryptochromes', 'Flowers', 'Gene Expression Regulation, Plant', 'Genes, Plant', 'Genes, Suppressor', 'Hypocotyl', 'Light', 'Models, Biological', 'Mutation', 'Phenotype', 'Photoperiod', 'Repressor Proteins', 'Suppression, Genetic', 'Time Factors', 'Transcription Factors']
| 21,296,763
|
[['G05.360.340.024.340.030'], ['B01.650.940.800.575.912.250.157.100'], ['D12.776.765.149'], ['D12.644.360.138.150', 'D12.776.331.180', 'D12.776.476.156.250', 'D12.776.765.593.500'], ['A18.024.249.500'], ['G05.308.375'], ['G05.360.340.024.340.393', 'G05.360.340.365.500'], ['G05.360.340.024.340.460'], ['A18.024.875.750', 'A18.024.937.249', 'A18.550.500'], ['G01.358.500.505.650', 'G01.590.540', 'G01.750.250.650', 'G01.750.770.578'], ['E05.599.395'], ['G05.365.590'], ['G05.695'], ['G01.910.675'], ['D12.776.260.703', 'D12.776.930.780'], ['G05.365.590.835', 'G05.558.835'], ['G01.910.857'], ['D12.776.930']]
|
['Phenomena and Processes [G]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
The impact of percutaneous tracheostomy on intensive care unit practice and training.
|
We studied the impact of introducing percutaneous tracheostomy to our intensive care unit on the incidence and timing of tracheostomy and on the implications for surgical training. The proportion of patients receiving intensive care who underwent tracheostomy doubled from a median of 8.5% to 16.8% (p < 0.01) following the introduction of the percutaneous technique with the procedure being undertaken significantly earlier during the intensive care stay. The opportunity for surgical trainees to gain experience in open surgical tracheostomy has been virtually lost. The increase in tracheostomy rate may reflect a previous under-utilisation caused by the logistic problems of transferring a critically ill patient to theatre, or alternatively a relaxation of the indications for tracheostomy caused by a perceived benefit for the patient. An increased workload may also have contributed to the rise. Surgical trainees should be encouraged to learn percutaneous techniques and training opportunities in open surgical techniques should be maximised.
|
['Critical Care', 'Education, Medical, Graduate', 'England', 'General Surgery', 'Humans', 'Medicine', 'Retrospective Studies', 'Specialization', 'Time Factors', 'Tracheostomy', 'Workload']
| 10,215,717
|
[['E02.760.190', 'N02.421.585.190'], ['I02.358.337.350', 'I02.358.399.350'], ['Z01.542.363.300'], ['H02.403.810.300'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['H02.403'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['H02.811'], ['G01.910.857'], ['E02.041.750', 'E04.579.935', 'E04.580.900', 'E04.928.780'], ['I03.946.225.500', 'N04.452.677.650.500']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Geographicals [Z]', 'Disciplines and Occupations [H]', 'Organisms [B]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 1
| 1
|
Developmental regulation of RNA editing and polyadenylation in four life cycle stages of Trypanosoma congolense.
|
The accumulation of many edited mRNAs is developmentally regulated in a transcript-specific fashion in Trypanosoma brucei. In addition, these transcripts are frequently present in two size classes which differ substantially in the lengths of their poly(A) tails, and poly(A) tail length is also developmentally regulated. Previously, these phenomena have only been studied in the mammalian bloodstream and insect procyclic forms (BF and PF, respectively) of T. brucei. In this paper, we examine developmental regulation of edited RNA abundance and poly(A) tail length of 3 mitochondrially encoded RNAs in mammalian BF and 3 insect stages (PF, epimastigotes, and metacyclics) of T. congolense. T. congolense BF and PF are similar, but not identical, to these stages of T. brucei with regard to edited RNA accumulation and poly(A) tail length. At the level of edited RNA, both epimastigotes and metacyclic stage parasites appear to be pre-adapted for the respiratory mechanisms of BF but not yet down-regulated from the cytochrome-based respiration of PF since edited RNAs encoding NADH dehydrogenase components are up-regulated and edited CYb RNA is abundant in these stages. Poly(A) tail lengths of mitochondrial mRNAs appear to be regulated independently of edited RNA abundance. These results indicate that multiple mechanisms for regulation of mitochondrial gene expression are active throughout the trypanosome life cycle.
|
['Animals', 'Apoproteins', 'Blotting, Northern', 'Cytochrome b Group', 'Cytochromes b', 'Gene Expression Regulation, Developmental', 'Mitochondria', 'NADH Dehydrogenase', 'Poly A', 'RNA Editing', 'RNA, Messenger', 'RNA, Protozoan', 'Trypanosoma brucei brucei', 'Trypanosoma congolense']
| 7,739,675
|
[['B01.050'], ['D12.776.070'], ['E05.196.401.095', 'E05.301.300.074', 'E05.601.100'], ['D08.244.187', 'D12.776.422.220.187'], ['D08.244.187.249', 'D12.776.422.220.187.249'], ['G05.308.310'], ['A11.284.430.214.190.875.564', 'A11.284.835.626'], ['D08.811.682.608.504.500', 'D12.776.157.427.374.375.863.500', 'D12.776.331.887', 'D12.776.556.579.374.375.140.500'], ['D13.695.578.550.500'], ['G02.111.760.250', 'G03.839.250', 'G05.308.700.250'], ['D13.444.735.544'], ['D13.444.735.650'], ['B01.268.475.868.887.080'], ['B01.268.475.868.887.128']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Sickness presenteeism predicts suboptimal self-rated health and sickness absence: a nationally representative study of the Swedish working population.
|
BACKGROUND: Earlier studies have suggested that sickness presenteeism (SP) may be a risk factor for future health problems. The purpose of the present study was to test this in a nationally representative prospective study of Swedish workers.METHODS: Prospective cohort with a representative sample of the Swedish working population surveyed in 2008 and 2010. Odds ratios (OR) with 95% confidence intervals (CI) were calculated using logistic regression.RESULTS: Those who reported more than 7 days of SP had higher risk of suboptimal SRH compared to those who reported no SP (OR = 5.95; 95% CI 4.98-7.12), also after adjustment for confounders (OR = 1.64; 95% CI 1.30-2.06). Those who reported 1-7 days of SP also had an increased risk before and after adjustments. Inclusion of self-rated physical and psychological work capacity did not attenuate the associations, whereas of emotional exhaustion attenuated the ORs to non-significance for both outcomes, indicating that the health consequences associated with SP are largely related to mental health.CONCLUSION: The results strengthen earlier findings suggesting that SP can be a risk factor for future suboptimal general health and sickness absence, particularly through mental health problems. This indicates that asking about SP could yield important information for employers, occupational health practitioners and GPs, possibly leading to more timely intervention that could decrease the risk of future sickness absence and more serious health problems, especially in the mental domain. Further studies of the possible causal pathways between SP and future health development are also warranted, especially since going to work is often seen as desirable also for those with poor health.
|
['Absenteeism', 'Adult', 'Cohort Studies', 'Female', 'Health Status', 'Humans', 'Male', 'Middle Aged', 'Occupational Health', 'Odds Ratio', 'Prospective Studies', 'Regression Analysis', 'Risk Factors', 'Sick Leave', 'Surveys and Questionnaires', 'Sweden']
| 22,984,547
|
[['F02.784.692.107'], ['M01.060.116'], ['E05.318.372.500.750', 'N05.715.360.330.500.750', 'N06.850.520.450.500.750'], ['I01.240.425', 'N01.224.425', 'N06.850.505.400.425'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['N01.400.525'], ['E05.318.740.600.600', 'G17.680.500', 'N05.715.360.750.625.590', 'N06.850.520.830.600.600'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['E05.318.740.750', 'N05.715.360.750.695', 'N06.850.520.830.750'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['N01.824.417.700.662', 'N04.452.677.800.662'], ['E05.318.308.980', 'N05.715.360.300.800', 'N06.850.520.308.980'], ['Z01.542.816.500']]
|
['Psychiatry and Psychology [F]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Geographicals [Z]']
| 0
| 1
| 0
| 0
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 1
|
Morbidity and healthcare utilisation of children in households with one adult: comparative observational study.
|
OBJECTIVE: To identify and consider differences in morbidity in children in households with one adult presenting to general practitioners compared with children in households with more than one adult.DESIGN: Observational study; data analysed with logistic regression controlling for age, sex, and practice.SUBJECTS: 93 356 children aged 0-15 years included in the fourth national study of morbidity in general practice and for whom data about household structure were available. Among them 10 983 (11.8%) were living in households with a sole adult.METHODS: Morbidity data were recorded from each consultation as the assessment diagnosis made by the general practitioner.MAIN OUTCOME MEASURES: Number of consultations and consultations per person for any illness, infections, acute respiratory infections, asthma, and accidents; number presenting and mean consultations per person for immunisation; number receiving home visits and home visits per person visited; average annual frequency of consultation among those consulting.RESULTS: Compared with children in other households, a higher proportion of children in households with one adult consulted for infections and accidents. The proportion consulting for immunisation was lower and the proportion receiving home visits greater. Mean numbers of consultations per person consulting were also generally higher for all conditions. For infections, accidents, and home visits, the differences were evident in all age groups.CONCLUSIONS: The study confirms the importance of single parent families as an indicator of deprivation. Children in such families should be targeted for immunisation and accident prevention.
|
['Adolescent', 'Adult', 'Child', 'Child Health Services', 'Child, Preschool', 'Family Practice', 'Female', 'House Calls', 'Humans', 'Infant', 'Infant, Newborn', 'Male', 'Morbidity', 'Multivariate Analysis', 'Patient Acceptance of Health Care', 'Rural Health', 'Single Parent', 'Social Class', 'United Kingdom', 'Urban Health', 'Workload']
| 9,596,597
|
[['M01.060.057'], ['M01.060.116'], ['M01.060.406'], ['N02.421.143.130'], ['M01.060.406.448'], ['H02.403.340.500'], ['N04.452.758.307'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703'], ['M01.060.703.520'], ['E05.318.308.985.525', 'N01.224.935.597', 'N06.850.505.400.975.525', 'N06.850.520.308.985.525'], ['E05.318.740.150.500', 'N05.715.360.750.125.500', 'N06.850.520.830.150.500'], ['F01.100.150.750.500', 'F01.145.488.887.500', 'N05.300.150.800.500'], ['N01.400.548.750'], ['F01.829.263.315.500.725.700', 'F01.829.263.500.320.785', 'I01.240.361.500.725.700', 'I01.880.853.150.423.500.725.700', 'I01.880.853.150.500.340.785', 'M01.620.785', 'N01.224.361.500.725.700', 'N01.824.308.500.725.700'], ['I01.880.853.996.755', 'N01.824.782'], ['Z01.542.363'], ['N01.400.548.875'], ['I03.946.225.500', 'N04.452.677.650.500']]
|
['Named Groups [M]', 'Health Care [N]', 'Disciplines and Occupations [H]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Psychiatry and Psychology [F]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Geographicals [Z]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 1
| 1
|
MYO6 is targeted by Salmonella
|
To establish infections, Salmonella injects virulence effectors that hijack the host actin cytoskeleton and phosphoinositide signaling to drive pathogen invasion. How effectors reprogram the cytoskeleton network remains unclear. By reconstituting the activities of the Salmonella effector SopE, we recapitulated Rho GTPase-driven actin polymerization at model phospholipid membrane bilayers in cell-free extracts and identified the network of Rho-recruited cytoskeleton proteins. Knockdown of network components revealed a key role for myosin VI (MYO6) in Salmonella invasion. SopE triggered MYO6 localization to invasion foci, and SopE-mediated activation of PAK recruited MYO6 to actin-rich membranes. We show that the virulence effector SopB requires MYO6 to regulate the localization of PIP3 and PI(3)P phosphoinositides and Akt activation. SopE and SopB target MYO6 to coordinate phosphoinositide production at invasion foci, facilitating the recruitment of cytoskeleton adaptor proteins to mediate pathogen uptake.
|
['Bacterial Proteins', 'Cell Membrane', 'Cytoskeleton', 'HeLa Cells', 'Host-Pathogen Interactions', 'Humans', 'Microfilament Proteins', 'Myosin Heavy Chains', 'Phosphatidylinositol 3-Kinase', 'Phosphatidylinositols', 'Salmonella typhimurium', 'Signal Transduction', 'Virulence Factors']
| 28,348,208
|
[['D12.776.097'], ['A11.284.149'], ['A11.284.430.214.190.750'], ['A11.251.210.190.400', 'A11.251.860.180.400', 'A11.436.340'], ['G06.462', 'G16.527.200'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D05.750.078.730', 'D12.776.220.525'], ['D05.750.078.730.475.100', 'D08.811.277.040.025.193.750.249', 'D12.776.210.500.600.100', 'D12.776.220.525.475.100'], ['D08.811.913.696.620.500.100'], ['D10.570.755.375.760.400.942'], ['B03.440.450.425.800.200.825', 'B03.660.250.150.710.160.760'], ['G02.111.820', 'G04.835'], ['D23.946.896']]
|
['Chemicals and Drugs [D]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Biologically active dichapetalins from Dichapetalum gelonioides.
|
A phytochemical investigation of the toxic tropical plant Dichapetalum gelonioides led to the isolation and identification of 14 new dichapetalins (1-14) and the known dichapetalins A (15) and K (16). The structures of the new compounds were determined by analyses of their NMR, MS, electronic circular dichroism, and X-ray diffraction data. The esterification at C-25 by 4-hydroxyphenylpropanoic acid and the hydroxylation at C-2' are unique in this unusual class of natural products. In addition to the known cytotoxicity, an array of biological activities, including antifeedant, nematicidal, antifungal, and NO and AChE inhibitory activities, were observed for this class of compounds. These findings suggested that dichapetalin hybrid triterpenoids as a class have broad biologically active cellular functions including defense against insect herbivores and pathogens.
|
['Antifungal Agents', 'Antinematodal Agents', 'Antineoplastic Agents', 'Cholinesterase Inhibitors', 'Feeding Behavior', 'Heterocyclic Compounds, 4 or More Rings', 'Magnoliopsida', 'Molecular Structure', 'Nitric Oxide', 'Spiro Compounds', 'Triterpenes']
| 24,597,894
|
[['D27.505.954.122.136'], ['D27.505.954.122.250.075.080'], ['D27.505.954.248'], ['D27.505.519.389.275', 'D27.505.519.625.120.300', 'D27.505.696.577.120.300'], ['F01.145.113.547', 'F01.145.407', 'G07.203.650.353'], ['D03.633.400'], ['B01.650.940.800.575.912.250'], ['G02.111.570', 'G02.466'], ['D01.339.387', 'D01.625.550.500', 'D01.625.700.500', 'D01.650.550.587.600'], ['D02.455.426.779', 'D04.711'], ['D02.455.849.919']]
|
['Chemicals and Drugs [D]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
A Case of Cap Polyposis with Epidermal Nevus in an Infant.
|
Cap polyposis is extremely rare in children. We report a case of an 11-month-old male infant who visited our hospital because of rectal prolapse and small amount of hematochezia lasting several days. He also had an epidermal nevus in the sacral area. Colonoscopy showed erythematous, multilobulated, circumferential, polypoid lesions with mucoid discharge from the rectum. He was diagnosed with cap polyposis by endoscopy and histologic examination. He was treated with surgical resection, and was closely followed up. In the relevant literature, there is no report of cap polyposis in an infant. We report the first case of cap polyposis in the youngest infant.
|
['Anti-Inflammatory Agents, Non-Steroidal', 'Colonic Polyps', 'Colonoscopy', 'Humans', 'Infant', 'Male', 'Mesalamine', 'Nevus', 'Polyps', 'Rectal Diseases', 'Rectum', 'Tomography, X-Ray Computed']
| 28,378,565
|
[['D27.505.696.663.850.014.040.500', 'D27.505.954.158.030', 'D27.505.954.329.030'], ['C23.300.825.411.235'], ['E01.370.372.250.250.200', 'E01.370.388.250.250.250.160', 'E04.210.240.250.160', 'E04.502.250.250.250.160'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703'], ['D02.241.223.100.050.300.500', 'D02.241.223.100.300.595.100.540', 'D02.241.511.390.595.100.540', 'D02.455.426.559.389.127.020.452.750', 'D02.455.426.559.389.127.281.595.100.540', 'D02.455.426.559.389.657.410.595.100.540'], ['C04.557.665.560'], ['C23.300.825'], ['C06.405.469.860'], ['A03.556.124.526.767', 'A03.556.249.249.767'], ['E01.370.350.350.810', 'E01.370.350.600.350.700.810', 'E01.370.350.700.700.810', 'E01.370.350.700.810.810', 'E01.370.350.825.810.810']]
|
['Chemicals and Drugs [D]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Named Groups [M]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
provider venture capital funds: investing in innovation.
|
As health systems continue to embrace disruptive innovation, they are increasingly likely to consider making a move into venture capital. Working in venture capital can benefit a health system in several ways, including: Allowing it to operate outside of bureaucracy and align projects with its core values. Encouraging innovation within the organization. Enabling it to respond quickly to changes in the market.
|
['Capital Financing', 'Delivery of Health Care', 'Financial Management', 'Humans', 'Organizational Innovation', 'United States']
| 27,382,708
|
[['N03.219.463.085'], ['N04.590.374', 'N05.300'], ['N03.219.463'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['N04.452.610'], ['Z01.107.567.875']]
|
['Health Care [N]', 'Organisms [B]', 'Geographicals [Z]']
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
|
[Composite-bonded bridges reinforced with Ribbond THM ribbon: an alternative of choice to other temporary prosthetic methods].
|
In the presence of anterior unitary tooth loss, the therapeutical arsenal of the practitioner remains the implant solution. But, at the young patients who have not completed their growth, it's necessary to pass by a transitional stage. The bonded bridges reinforced by a polyethylene fibbers splint such as Ribbond THM constitute an alternative of choice to solve the aesthetic damage in the short and medium term while waiting for the prosthesis with implant. It's a similar case that is reported through this article.
|
['Adolescent', 'Composite Resins', 'Dental Materials', 'Denture, Partial, Fixed, Resin-Bonded', 'Denture, Partial, Temporary', 'Esthetics, Dental', 'Female', 'Humans', 'Incisor', 'Polyethylenes', 'Tooth Loss']
| 15,074,071
|
[['M01.060.057'], ['D05.750.716.822.308', 'D25.339.816.500', 'D25.720.716.822.308', 'J01.637.051.339.816.500', 'J01.637.051.720.716.822.308'], ['D25.339', 'D27.720.102.339', 'J01.637.051.339'], ['E06.780.346.760.943.271.260', 'E07.695.190.200.220.220.220'], ['E06.780.346.760.943.484', 'E07.695.190.200.220.235'], ['E06.420'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A14.549.167.860.425'], ['D02.455.326.271.665.550', 'D05.750.716.507', 'D25.720.716.507', 'J01.637.051.720.716.507'], ['C07.465.714.804', 'C07.793.870']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Technology, Industry, and Agriculture [J]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Anatomy [A]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 1
| 0
| 0
|
Urethral advancement, glanduloplasty and preputioplasty in distal hypospadias.
|
One hundred and thirty boys with subcoronal (78) and subglandular (52) hypospadias were submitted to Urethral Advancement, Glanduloplasty and Preputioplasty (URAGP). The main steps of the operation were the following: blunt dissection and mobilization of the urethra as extensively as needed to reach the tip of the glans without tension; embedding of the urethra into a groove of the glans created by excision of tissue between two incisions converging from each side of the primary meatal location up to the tip of the glans; fixation of the meatus up to the tip of the glans and suturing of the glandular flaps over the mobilized urethra; shaping the foreskin to normal appearance. Reconstruction was uncomplicated in 117 patients (90%). Postoperative fistulae and urethral retraction to an abnormal position occurred in 7 and 6 cases respectively. All meatal retractions and 2 fistulae next to the meatus were dealt with successfully using the Mathieu procedure. On the basis of this experience we conclude that the technique is applicable to any type of distal hypospadias regardless of the severity of glandular ventriflexion, it is amenable to the Mathieu procedure in case of failure and gives good functional and cosmetic results.
|
['Child', 'Child, Preschool', 'Humans', 'Hypospadias', 'Male', 'Penis', 'Postoperative Complications', 'Suture Techniques', 'Treatment Outcome', 'Urethra']
| 8,773,225
|
[['M01.060.406'], ['M01.060.406.448'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C12.294.494.400', 'C12.706.516', 'C13.351.875.466', 'C16.131.939.516'], ['A05.360.444.492'], ['C23.550.767'], ['E04.987.775'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['A05.360.444.492.726', 'A05.810.876']]
|
['Named Groups [M]', 'Organisms [B]', 'Diseases [C]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Childhood IQ predicts age-38 oral disease experience and service-use.
|
OBJECTIVES: Given that people with higher intelligence have been shown to live longer, enjoy better health and have more favourable health behaviours, we investigated the association between childhood IQ and a range of important dental health and service-use indicators at age 38.METHODS: Long-standing prospective study of a complete birth cohort, with childhood IQ (assessed at ages 7, 9, 11 and 13 years) used to allocate participants (N = 818) to one of four ordinal categories of childhood IQ.RESULTS: There were distinct and consistent gradients by childhood IQ in almost all of the dental caries experience measures (with the exception of filled teeth) whereby each was most severe in the lowest child IQ category and least severe in the highest; the exception was the mean FT score, for which there was no discernible gradient. Indicators of self-care and periodontal disease experience showed similar gradients, and multivariate modelling using the continuous IQ score confirmed the observed patterns.CONCLUSIONS: Childhood cognitive function is a key determinant of oral health and dental service-use by midlife, with those of lower cognitive capacity as children likely to have poorer oral health, less favourable oral health-related beliefs, and more detrimental self-care and dental visiting practices by age 38. There is a need to shape dental clinical services and public health interventions so that people with the poorest cognitive function do not continue to be disadvantaged.
|
['Adolescent', 'Adult', 'Child', 'Dental Care', 'Dental Caries', 'Humans', 'Intelligence', 'Oral Health', 'Periodontal Diseases', 'Prospective Studies']
| 30,812,053
|
[['M01.060.057'], ['M01.060.116'], ['M01.060.406'], ['E06.170', 'N02.421.240.190'], ['C07.793.720.210'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F01.752.543'], ['N01.400.535'], ['C07.465.714'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Diseases [C]', 'Organisms [B]', 'Psychiatry and Psychology [F]']
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Endothelin-1 gene and endothelial nitric oxide synthase gene polymorphisms in adolescents with juvenile and obesity-associated hypertension.
|
Hypertension is an increasing public health problem all over the world. Essential hypertension accounts for more than 90% of cases of hypertension. It is a complex genetic, environmental and demographic trait. New method in molecular biology has been proposed a number of candidate genes, but the linkage or association with hypertension has been problematic (lack of gene-gene and gene-environment interaction). It is well known that genetic influences are more important in younger hypertensives, because children are relatively free from the common environmental factors contributing to essential hypertension. The association studies compare genotype ferquencies of the candidate gene between patient groups and the controls, in pathways known to be involved in blood pressure regulation. This study examined three polymorphisms of these factors encoding genes (ET-1 G+5665T (Lys198Asn), endothelial nitric oxide synthase (eNOS) T-786C promoter polymorphism and 27-bp repeat polymorphism in intron 4) in adolescents with juvenile essential and obesity-associated hypertension. Significant differences were found in the G/T genotype of the ET-1 polymorphism in the hypertensive and obese+hypertensive patients (body mass index (BMI) > 30). A strong association was detected between the BMI and the polymorphism of the ET-1 gene. It seems that ET-1 gene polymorphism plays a role in the development of juvenile hypertension associated with obesity. Although no significant differences were seen in the case of the eNOS promoter polymorphism and the eNOS 4th intron 27-bp repeat polymorphism. It seems that eNOS may play a role, but this is not the main factor in the control of blood pressure; it is rather a fine regulator in this process. This study with adolescents facilitates an understanding of the genetic factors promoting juvenile hypertension and obesity.
|
['Adolescent', 'Body Mass Index', 'Child', 'Endothelin-1', 'Gene Frequency', 'Humans', 'Hypertension', 'Male', 'Nitric Oxide Synthase Type III', 'Nitrogen Oxides', 'Obesity', 'Polymorphism, Genetic']
| 17,444,275
|
[['M01.060.057'], ['E01.370.600.115.100.125', 'E05.041.124.125', 'G07.100.100.125', 'N06.850.505.200.100.175'], ['M01.060.406'], ['D12.644.276.400.225', 'D12.776.467.400.225', 'D23.529.400.225'], ['G05.330'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C14.907.489'], ['D08.811.682.664.500.772.750'], ['D01.362.635', 'D01.625.550', 'D01.650.550.587'], ['C18.654.726.500', 'C23.888.144.699.500', 'E01.370.600.115.100.160.120.699.500', 'G07.100.100.160.120.699.500'], ['G05.365.795']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
TP53
|
Emergency (stress) granulopoiesis is an episodic process for the production of granulocytes in response to infectious challenge. We previously determined that Fanconi C, a component of the Fanconi DNA-repair pathway, is necessary for successful emergency granulopoiesis. Fanconi anemia results from mutation of any gene in this pathway and is characterized by bone marrow failure (BMF) in childhood and clonal progression in adolescence. Although murine Fanconi anemia models exhibit relatively normal steady-state hematopoiesis, FANCC-/- mice are unable to mount an emergency granulopoiesis response. Instead, these mice develop BMF and die during repeated unsuccessful emergency granulopoiesis attempts. In FANCC-/- mice, BMF is associated with extensive apoptosis of hematopoietic stem and progenitor cells through an undefined mechanism. In this study, we find that TP53 haploinsufficiency completely rescues emergency granulopoiesis in FANCC-/- mice and protects them from BMF during repeated emergency granulopoiesis episodes. Instead, such recurrent challenges accelerated clonal progression in FANCC-/-TP53+/- mice. In FANCC-/- mice, BMF during multiple emergency granulopoiesis attempts was associated with increased ataxia telangiectasia and Rad3-related protein (Atr) and p53 activation with each attempt. In contrast, we found progressive attenuation of expression and activity of Atr, and consequent p53 activation and apoptosis, in the bone marrow of FANCC-/-TP53+/- mice during this process. Therefore, activation of Atr-with consequent Fanconi-mediated DNA repair or p53-dependent apoptosis-is an essential component of emergency granulopoiesis and it protects the bone marrow from genotoxic stress during this process.
|
['Animals', 'Apoptosis', 'Ataxia Telangiectasia Mutated Proteins', 'Bone Marrow', 'DNA Damage', 'DNA Repair', 'Fanconi Anemia', 'Fanconi Anemia Complementation Group C Protein', 'Granulocytes', 'Haploinsufficiency', 'Leukopoiesis', 'Mice', 'Tumor Suppressor Protein p53']
| 29,427,417
|
[['B01.050'], ['G04.146.954.035'], ['D08.811.913.696.620.682.700.097', 'D12.776.157.687.125', 'D12.776.660.720.125'], ['A15.382.216'], ['G05.200'], ['G02.111.222', 'G05.219'], ['C15.378.071.085.080.280', 'C15.378.190.223.500.500.280', 'C16.320.077.280', 'C18.452.284.280'], ['D12.776.313.750'], ['A11.118.637.415', 'A11.148.350', 'A11.627.340', 'A15.145.229.637.415', 'A15.378.316.340', 'A15.382.490.315'], ['G05.365.590.029.530.587', 'G05.380.350.500'], ['G04.152.825.597', 'G09.188.343.597'], ['B01.050.150.900.649.313.992.635.505.500'], ['D12.776.157.687.650', 'D12.776.260.820', 'D12.776.624.776.775', 'D12.776.660.720.650', 'D12.776.744.845']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
An open-label study assessing the safety and efficacy of alitretinoin in patients with severe chronic hand eczema unresponsive to topical corticosteroids.
|
BACKGROUND: Blinded, controlled studies have found that oral alitretinoin is well tolerated and effective in the treatment of severe chronic hand eczema (CHE).AIM: To assess the safety and efficacy of oral alitretinoin in patients with severe CHE in an open-label study using flexible dosing and a new measurement of patient-relevant benefits.METHODS: In total, 249 patients aged 18-75 years with severe CHE unresponsive to treatment with topical corticosteroids received alitretinoin 30 mg once daily for up to 24 weeks. Safety assessments included adverse events (AEs) and laboratory tests. Efficacy assessments included Physician's Global Assessment (PGA), the Modified Total Lesion Symptom Score, Patient's Global Assessment and extent of disease, as well as intensity of pain and pruritus as determined by visual analogue scale (VAS) and a categorical scale for pruritus.RESULTS: Alitretinoin was well tolerated when given for up to 24 weeks. Dose reduction occurred in 16.5% of patients. Dose interruption was required for 15.7% of patients, most commonly for headache. AEs and laboratory changes comprised effects typical of the retinoid class. A PGA response of 'clear' or 'almost clear' hands was reported for 46.6% of patients, similar to the response rate seen in blinded trials. Results of VAS and categorical assessments of pruritus provided supporting evidence of efficacy, and treatment was assessed as providing meaningful benefits to patients.CONCLUSIONS: Oral alitretinoin 30 mg was well tolerated and effective, and provided distinct therapeutic benefits in severe CHE, as assessed by patients.
|
['Administration, Oral', 'Adolescent', 'Adult', 'Aged', 'Alitretinoin', 'Chronic Disease', 'Dermatologic Agents', 'Drug Administration Schedule', 'Female', 'Glucocorticoids', 'Hand Dermatoses', 'Humans', 'Male', 'Middle Aged', 'Severity of Illness Index', 'Treatment Failure', 'Treatment Outcome', 'Tretinoin', 'Young Adult']
| 21,070,335
|
[['E02.319.267.100'], ['M01.060.057'], ['M01.060.116'], ['M01.060.116.100'], ['D02.455.326.271.665.202.495.818.500.500', 'D02.455.426.392.368.367.379.249.700.860.500.500', 'D02.455.849.131.495.818.800.500', 'D02.455.849.291.925.500.500', 'D23.767.261.700.780.500'], ['C23.550.291.500'], ['D27.505.954.444'], ['E02.319.283'], ['D06.472.040.543', 'D27.505.696.399.472.488'], ['C17.800.338'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['E05.318.308.980.438.475.456.500', 'N05.715.360.300.800.438.375.364.500', 'N06.850.520.308.980.438.475.364.500'], ['E01.789.800.760', 'N04.761.559.590.800.760', 'N05.715.360.575.575.800.760'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['D02.455.326.271.665.202.495.818.500', 'D02.455.426.392.368.367.379.249.700.860.500', 'D02.455.849.131.495.818.800', 'D02.455.849.291.925.500', 'D23.767.261.700.780'], ['M01.060.116.815']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Named Groups [M]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Organisms [B]', 'Health Care [N]']
| 0
| 1
| 1
| 1
| 1
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Covalent immobilization of recombinant organophosphorus hydrolase on spores of Bacillus subtilis.
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AIMS: Organophosphorus pesticides are widely used in agriculture. Accordingly, decontamination of these pesticides and their residual in environment is an important aim of researchers. One of the best approaches is enzymatic detoxification of these compounds with organophosphorus hydrolase (OPH). The immobilization of OPH on environmentally friendly supports is of great importance for developing stabilized enzymes for degradation of organophosphorus compounds.METHODS AND RESULTS: In this study, Bacillus subtilis spores were applied as a new matrix for immobilizing OPH for the first time; this enzyme was covalently bound to the spores by using EDC-NHS as coupling reagents and the immobilization was confirmed by enzymatic activity, Western blot, flow cytometry and fluorescence microscopic analysis. The immobilization yield was about 55% and the immobilized OPH hydrolysed paraoxon, an organophosphate substrate, without significant loss of activity was six times. The spores with immobilized OPH on their surface were successfully characterized using FT-IR analysis and SEM imaging. Thermal and pH stability was improved by immobilization of OPH on the spore surface.CONCLUSIONS: Owing to safety, environmentally friendly and low cost of spores, these spores can be employed in biosensors for monitoring and biodegradation of organophosphate contaminants in the environment and detoxification processes in bioreactors with high reusability without decrease in the activity.SIGNIFICANCE AND IMPACT OF THE STUDY: We believe that the spore, an environmentally friendly matrix, can be used for covalent immobilization of OPH efficiently and can be applied for detoxification of organophosphorus compounds under adverse environmental conditions.
|
['Aryldialkylphosphatase', 'Bacillus subtilis', 'Enzymes, Immobilized', 'Organophosphorus Compounds', 'Paraoxon', 'Pesticides', 'Recombinant Proteins', 'Spectroscopy, Fourier Transform Infrared', 'Spores, Bacterial']
| 25,565,038
|
[['D08.811.277.352.660.500'], ['B03.300.390.400.158.218.725', 'B03.353.500.100.218.725', 'B03.510.100.100.218.725', 'B03.510.415.400.158.218.725', 'B03.510.460.410.158.218.725'], ['D08.811.180', 'D12.776.463.500'], ['D02.705'], ['D02.705.400.650'], ['D27.720.031.700', 'D27.888.723'], ['D12.776.828'], ['E05.196.712.726.676.700', 'E05.196.867.826.676.700'], ['A11.870.700', 'B05.775.700']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
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Relational diagnosis: a coconstructive-developmental perspective on assessment and treatment.
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As we expand the borders of traditional diagnostic nomenclature, distinguishing health from disorder becomes complex. This is especially true when the diagnostic lens views individuals as they exist within families that reside within wider social milieus. From this viewpoint, the recursive and interdependent factors influencing how individuals, relationships, and wider collectives contribute to and construe disorder need to be determined Systemic Cognitive-Developmental Therapy is one approach to conducting viable relational diagnoses and treatment, guiding therapists to formulate an understanding of the internal cognitive-developmental resources available to clients and other relevant, interdependent systems, and to examine the forces influencing the interactive discourse across these systems. The constructs of worldview, information processing styles, power differentials, and interpersonal connectiveness are presented as conceptual tools to guide relational diagnosis. An example illustrates relational diagnosis and treatment.
|
['Adolescent', 'Adult', 'Child', 'Cognitive Behavioral Therapy', 'Family Therapy', 'Female', 'Humans', 'Interpersonal Relations', 'Male', 'Mental Disorders', 'Psychiatric Status Rating Scales', 'Psychotherapy', 'Systems Theory']
| 10,946,730
|
[['M01.060.057'], ['M01.060.116'], ['M01.060.406'], ['F04.754.137.350'], ['F04.754.864.581.273'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F01.829.401'], ['F03'], ['F04.711.513.653'], ['F04.754'], ['H01.770.808']]
|
['Named Groups [M]', 'Psychiatry and Psychology [F]', 'Organisms [B]', 'Disciplines and Occupations [H]']
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
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Subclinical hyperthyroid patients' knowledge about radioiodine therapy--the key role of medical information.
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OBJECTIVES: Many patients with a chronic disease are dissatisfied with the information they are given. A brief questionnaire completed by patients would assist health professionals to identify areas of information needed to be provided, tailored to the patient's mental condition.AIM: The aim of our study was to assess how often thyroid patients report being adequately informed about iodine treatment in connection with their real need thereof, emotional state and acceptance of the disease.METHODS: One hundred outpatients who had presented subclinical hyperthyroidism "[19 men (19%), 81 women (81%); mean (SD±) age 53±14,range 18-77 yr ] treated with radioiodine (RAI) responded to an Experimental Questionnaire, 54 of them answered to AIS, HADS-M and Beck Inventory measuring their acceptance of the illness and depressive symptoms, 37 of them answered the Patient Request Form (PRF).RESULTS: The obtained results indicated that about 50% of patients treated with 131I therapy did not receive suitable information about their treatment. Neither written information prepared by the specialist, nor verbal information given by physicians were adequate for specific problems of study group. The examined patients presented with a comparable intensity of three distinct types of requests: for explanation and reassurance, for emotional support, and for investigation and treatment. The acceptance of their disease was mediocre for most of the study group.CONCLUSION: We conclude that the reported lack of satisfaction with medical information in study group was associated with depressive symptoms influencing cognitive efficiency, patients' great need of emotional and cognitive support, influencing the acceptance of their disease, and social prejudice to radioiodine (as a method of treatment), worrying them additionally. All thyroid patients even these with subclinical symptoms of hyperthyroidism should be treated with specific attention by physicians, especially during information process.
|
['Adult', 'Aged', 'Depression', 'Female', 'Health Knowledge, Attitudes, Practice', 'Humans', 'Hyperthyroidism', 'Informed Consent', 'Iodine Radioisotopes', 'Male', 'Middle Aged', 'Patients', 'Poland', 'Surveys and Questionnaires', 'Treatment Outcome', 'Young Adult']
| 21,196,911
|
[['M01.060.116'], ['M01.060.116.100'], ['F01.145.126.350'], ['F01.100.150.500', 'N05.300.150.410'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C19.874.397'], ['I01.880.604.473.650.718', 'I01.880.604.583.427', 'N03.706.437.650.312', 'N03.706.535.489'], ['D01.268.380.400.500.496', 'D01.496.448.496', 'D01.496.749.474'], ['M01.060.116.630'], ['M01.643'], ['Z01.542.248.679'], ['E05.318.308.980', 'N05.715.360.300.800', 'N06.850.520.308.980'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Psychiatry and Psychology [F]', 'Health Care [N]', 'Organisms [B]', 'Diseases [C]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Chemicals and Drugs [D]', 'Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 1
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[An Examination of Colon Cancer Treatment Policies for the Elderly].
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Currently, the rate of aging is increasing rapidly and the number of elderly patients in hospitals is rising in Japan. Under such circumstances, we established an emergency care unit for the elderly at our hospital in July 2014 to provide acute healthcare of the community. Our unit has been active in providing treatment for acute diseases in elderly individuals in the community. We are also pursuing continuous medical care for elderly individuals in collaboration with the community comprehensive care unit at our hospital and other facilities in the community, such as care facilities for the elderly and specialist elderly nursing homes. From January to December 2015, we examined treatment policies for colon cancer at our hospital. The presence or absence of dementia and patient's age are factors considered before treatment at our hospital; however, in many cases colon cancer was treated using surgery if the patient's generalcondition permitted it. In colon cancer patients, this takes into consideration the decreased quality of life resulting from losing the ability to eat due to bleeding and intestinal obstruction. We hereby report specific cases of colon cancer treatment policies for elderly patients at our hospital, together with a discussion of the literature.
|
['Aged', 'Aged, 80 and over', 'Colectomy', 'Colonic Neoplasms', 'Dementia', 'Female', 'Humans', 'Male', 'Prognosis']
| 28,133,044
|
[['M01.060.116.100'], ['M01.060.116.100.080'], ['E04.210.219'], ['C04.588.274.476.411.307.180', 'C06.301.371.411.307.180', 'C06.405.249.411.307.180', 'C06.405.469.158.356.180', 'C06.405.469.491.307.180'], ['C10.228.140.380', 'F03.615.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.789']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
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Status of New Vaccine Introduction - Worldwide, September 2016.
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Since the global Expanded Program on Immunization (EPI) was launched in 1974, vaccination against six diseases (tuberculosis, polio, diphtheria, tetanus, pertussis, and measles) has prevented millions of deaths and disabilities (1). Significant advances have been made in the development and introduction of vaccines, and licensed vaccines are now available to prevent 25 diseases (2,3). Historically, new vaccines only became available in low-income and middle-income countries decades after being introduced in high-income countries. However, with the support of global partners, including the World Health Organization (WHO) and the United Nations Children's Fund, which assist with vaccine prequalification and procurement, as well as Gavi, the Vaccine Alliance (Gavi) (4), which provides funding and shapes vaccine markets through forecasting and assurances of demand in low-income countries in exchange for lower vaccine prices, vaccines are now introduced more rapidly. Based on data compiled in the WHO Immunization Vaccines and Biologicals Database* (5), this report describes the current status of introduction of Haemophilus influenzae type b (Hib), hepatitis B, pneumococcal conjugate, rotavirus, human papillomavirus, and rubella vaccines, and the second dose of measles vaccine. As of September 2016, a total of 191 (99%) of 194 WHO member countries had introduced Hib vaccine, 190 (98%) had introduced hepatitis B vaccine, 132 (68%) had introduced pneumococcal conjugate vaccine (PCV), and 86 (44%) had introduced rotavirus vaccine into infant vaccination schedules. Human papillomavirus vaccine (HPV) had been introduced in 67 (35%) countries, primarily targeted for routine use in adolescent girls. A second dose of measles-containing vaccine (MCV2) had been introduced in 161 (83%) countries, and rubella vaccine had been introduced in 149 (77%). These efforts support the commitment outlined in the Global Vaccine Action Plan (GVAP), 2011-2020 (2), endorsed by the World Health Assembly in 2012, to extend the full benefits of immunization to all persons.
|
['Adolescent', 'Bacterial Capsules', 'Child', 'Child, Preschool', 'Female', 'Global Health', 'Haemophilus Vaccines', 'Hepatitis B Vaccines', 'Humans', 'Immunization Programs', 'Immunization Schedule', 'Infant', 'Infant, Newborn', 'Measles Vaccine', 'Papillomavirus Vaccines', 'Pneumococcal Vaccines', 'Rotavirus Vaccines', 'Rubella Vaccine', 'Vaccination', 'Vaccines, Conjugate']
| 27,764,083
|
[['M01.060.057'], ['A20.186'], ['M01.060.406'], ['M01.060.406.448'], ['H02.403.371', 'N01.400.337'], ['D20.215.894.135.450'], ['D20.215.894.899.955.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['N02.421.726.608'], ['E02.095.465.425.400.470', 'E05.478.550.545'], ['M01.060.703'], ['M01.060.703.520'], ['D20.215.894.899.404'], ['D20.215.894.899.498'], ['D20.215.894.135.750.600'], ['D20.215.894.899.760'], ['D20.215.894.899.779'], ['E02.095.465.425.400.530.890', 'E05.478.550.600.890', 'N02.421.726.758.310.890', 'N06.850.780.200.425.900', 'N06.850.780.680.310.890'], ['D20.215.894.865.900', 'D23.050.865.900']]
|
['Named Groups [M]', 'Anatomy [A]', 'Disciplines and Occupations [H]', 'Health Care [N]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 1
| 0
|
Vulnerability to AIDS among the elderly in an urban center in central Brazil.
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OBJECTIVE: As the world population ages with an improved quality of life and sexual longevity, the prevalence of AIDS is rising among the elderly. The purpose of this study was to estimate the vulnerability to AIDS among individuals attending senior community centers in Campo Grande, Mato Grosso do Sul, Brazil.METHOD: This descriptive, exploratory investigation included 329 subjects selected in a probabilistic manner. Individuals with scores on the Mini-Mental State Examination indicating cognitive impairment were excluded from the analyses. Barthel's and Lawton's functional assessment scales were applied. Interviews were conducted to evaluate the presence of cognitive and behavioral factors associated with HIV transmission.RESULTS: Most subjects were non-dependent, fell within the 60- to 69-year age bracket and were female. A majority of individuals reported having knowledge about AIDS and were aware that the elderly are vulnerable to the disease. More than a quarter (26.9%) of the sample reported previous HIV testing. No participants reported drug use, homosexual orientation, or alcohol abuse. A minority of participants reported using medication for erectile dysfunction. Casual and multiple partners accounted for 12% and 34% of reported intercourse experiences, respectively. Condom use was reported by 14% of respondents.CONCLUSION: Unprotected sex was the primary factor accounting for vulnerability to AIDS among the elderly.
|
['Aged', 'Brazil', 'Chi-Square Distribution', 'Condoms', 'Cross-Sectional Studies', 'Female', 'Geriatric Assessment', 'HIV Infections', 'Health Knowledge, Attitudes, Practice', 'Humans', 'Male', 'Middle Aged', 'Risk Factors', 'Sex Distribution', 'Sexual Behavior', 'Unsafe Sex', 'Urban Population', 'Vulnerable Populations']
| 22,249,476
|
[['M01.060.116.100'], ['Z01.107.757.176'], ['E05.318.740.994.300', 'G17.820.300', 'N05.715.360.750.750.200', 'N06.850.520.830.994.300'], ['E07.190.270.150'], ['E05.318.372.500.875', 'N05.715.360.330.500.875', 'N06.850.520.450.500.875'], ['E05.318.308.225', 'I01.240.425.350', 'N01.224.425.350', 'N05.715.360.300.360', 'N06.850.505.400.425.350', 'N06.850.520.308.225'], ['C01.221.250.875', 'C01.221.812.640.400', 'C01.778.640.400', 'C01.925.782.815.616.400', 'C01.925.813.400', 'C20.673.480'], ['F01.100.150.500', 'N05.300.150.410'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['I01.240.800', 'N01.224.803', 'N06.850.505.400.850'], ['F01.145.802'], ['F01.145.802.987'], ['N01.600.900'], ['M01.965']]
|
['Named Groups [M]', 'Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 1
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Development-Dependent Changes in the NR2 Subtype of the N-Methyl-D-Aspartate Receptor in the Suprachiasmatic Nucleus of the Rat.
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The suprachiasmatic nucleus (SCN) is the main brain clock that regulates circadian rhythms in mammals. The SCN synchronizes to the LD cycle through the retinohypothalamic tract (RHT), which projects to ventral SCN neurons via glutamatergic synapses. Released glutamate activates N-methyl-D-aspartate (NMDA) receptors, which play a critical role in the activation of signaling cascades to enable phase shifts. Previous evidence indicates that presynaptic changes during postnatal development consist of an increase in RHT fibers impinging on SCN neurons between postnatal day (P) 1 to 4 and P15. The aim of this study was to evaluate postsynaptic developmental changes in the NR2 subunits that determine the pharmacological and biophysical properties of the neuronal NMDA receptors in the ventral SCN. To identify the expression of NR2 subtypes, we utilized RT-PCR, immunohistochemical fluorescence, and electrophysiological recordings of synaptic activity. We identified development-dependent changes in NR2A, C, and D subtypes in mRNA and protein expression, whereas NR2B protein was equally present at all analyzed postnatal ages. The NR2A antagonist PEAQX (100 nM) reduced the frequency of NMDA excitatory postsynaptic currents (EPSCs) at P8 significantly more than at P34, but the antagonists for NR2B (3 ìM Ro 25-6981) and NR2C/D (150 nM PPDA) did not influence NMDA EPSCs differently at the 2 analyzed postnatal ages. Our results point to P8 as the earliest analyzed postnatal age that shows mRNA and protein expression similar to those found at the juvenile stage P34. Taken together, our findings indicate that postsynaptic development-dependent modifications in the NR2 subtypes of the NMDA receptor could be important for the synchronization of ventral SCN neurons to the LD cycle at adult stages.
|
['Aging', 'Animals', 'Brain', 'Circadian Rhythm', 'Rats', 'Rats, Wistar', 'Receptors, N-Methyl-D-Aspartate', 'Suprachiasmatic Nucleus Neurons', 'Synapses']
| 30,654,688
|
[['G07.345.124'], ['B01.050'], ['A08.186.211'], ['G07.180.562.190'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.900'], ['D12.776.157.530.400.400.500.500', 'D12.776.543.550.450.500.200.500', 'D12.776.543.585.400.500.200.500', 'D12.776.543.750.720.200.450.400.500'], ['A08.186.211.180.497.342.625.500', 'A08.186.211.200.317.357.342.625.500', 'A08.675.947', 'A11.671.947'], ['A08.850', 'A11.284.149.165.420.780']]
|
['Phenomena and Processes [G]', 'Organisms [B]', 'Anatomy [A]', 'Chemicals and Drugs [D]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Application of strain and calibration of F?rster Resonance Energy Transfer (FRET) emission for in vitro live cell response to cytoskeletal deformation.
|
Cellular mechanotransduction is an integral part of many crucial physiological processes, but non-invasive tools for quantifying intracellular strain in vivo are not available for complex tissues such as bone. As a first step to address this gap, we have utilized a novel, non-invasive approach to quantify cellular strain in vitro by employing a transfected alpha-actinin F?rster Resonance Energy Transfer (FRET) sensor. Following validation experiments, mouse fibroblasts transfected to express FRET sensors were seeded to a silicone membrane and subjected to up to 10% tensile strain mounted on a multi-photon microscope. During tensile strain, fluorescent emission of acceptor (YFP) and donor (CFP) proteins was quantified. YFP/CFP ratio was normalized to the initial baseline (unstretched) ratio for each cell which demonstrates a negative linear correlation between the relative proximity ratio of emission spectra and cell strain, with a mean decrease of 1.017% normalized ratio for every percent strain experienced by the cell. The exciting implications of our findings are that the discovery of the stable correlation between loss of FRET and experimentally applied strain opens intriguing possibilities for future use of this technology with in vivo research, leading to discoveries improving disease treatments in mechanically sensitive tissues such as bone.
|
['Actinin', 'Animals', 'Biomechanical Phenomena', 'Calibration', 'Cell Survival', 'Cells, Cultured', 'Cytoskeleton', 'Female', 'Fluorescence Resonance Energy Transfer', 'Luminescent Proteins', 'Mechanotransduction, Cellular', 'Mice', 'Stress, Mechanical']
| 27,589,930
|
[['D05.750.078.730.248', 'D12.776.210.500.095', 'D12.776.220.525.250'], ['B01.050'], ['G01.154.090', 'G01.374.089'], ['E05.978.155'], ['G04.346'], ['A11.251'], ['A11.284.430.214.190.750'], ['E05.196.712.516.600.676.500', 'G01.154.240.280', 'G02.111.255.280'], ['D12.776.532'], ['G01.154.090.500', 'G02.111.820.580', 'G04.835.580'], ['B01.050.150.900.649.313.992.635.505.500'], ['G01.374.835']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Combination of topotecan and cisplatin in relapsed patients with small cell lung cancer: a phase II study of the hellenic cooperative oncology group (HeCOG).
|
PURPOSE: To assess the safety and efficacy of a 3-day schedule of cisplatin and topotecan in patients with recurrent small-cell lung cancer (SCLC).METHODS: Thirty-four relapsed patients were treated with cisplatin 20 mg/m2 and topotecan 0.9 mg/m2, both given on days 1-3 every 3 weeks, in a phase II study.RESULTS: Complete response (CR) was achieved in two patients (6%), partial response (PR) in 4 (12%), stable disease in 6 (18%) and progressive disease in 14 (41%). Eight patients (23%) were non-evaluable for response. Among 21 sensitive patients, 2 (9.5%) achieved CR and 3 (14%) PR. Among 13 refractory patients, none achieved CR and only 1 (8%) PR. Median survival was 6.5 months for all patients, 7.8 for sensitive and 6.2 for refractory. Median time to progression (TTP) was 4.4 months for all patients, 5.9 for sensitive and 3.2 for refractory. Grade 3-4 toxicities included anemia (15%), thrombocytopenia (15%), neutropenia (42%), nausea/vomiting (3%), and alopecia (6%). No toxic death occurred.CONCLUSIONS: This 3-day schedule was well tolerated, produced modest response rates but good survival and TTP both in sensitive and refractory patients with relapsed SCLC.
|
['Adult', 'Aged', 'Antineoplastic Combined Chemotherapy Protocols', 'Carcinoma, Small Cell', 'Cisplatin', 'Drug Administration Schedule', 'Female', 'Humans', 'Infusions, Intravenous', 'Lung Neoplasms', 'Male', 'Middle Aged', 'Survival Analysis', 'Topotecan', 'Treatment Outcome']
| 16,028,103
|
[['M01.060.116'], ['M01.060.116.100'], ['E02.183.750.500', 'E02.319.077.500', 'E02.319.310.037'], ['C04.557.470.200.380'], ['D01.210.375', 'D01.625.125', 'D01.710.100'], ['E02.319.283'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.319.267.082.500', 'E02.319.267.510.590'], ['C04.588.894.797.520', 'C08.381.540', 'C08.785.520'], ['M01.060.116.630'], ['E05.318.740.998', 'N05.715.360.750.795', 'N06.850.520.830.998'], ['D03.132.151.850'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Health Care [N]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Structurally related Spc1p and Spc2p of yeast signal peptidase complex are functionally distinct.
|
Two subunits of the mammalian signal peptidase complex, SPC12 and SPC25, share similar membrane topologies with the majority of each protein oriented toward the cytoplasm. Such similarities may suggest that these proteins perform redundant functions in signal peptidase activity. In the present study, we addressed this issue through analysis of the yeast homologs to SPC12 and SPC25, Spc1p and Spc2p. We show that both Spc1p and Spc2p are nonessential for signal peptidase activity and growth of yeast cells and that null mutations in the genes encoding Spc1p and Spc2p are synthetically lethal with a conditional mutation affecting Sec11p, an essential subunit of yeast signal peptidase. However, a high copy plasmid encoding Spc1p suppresses the conditional sec11 mutation, whereas the corresponding plasmid encoding Spc2p does not suppress sec11. Moreover, Spc2p, but not Spc1p, is important for signal peptidase activity and cell viability at high temperatures. These results indicate that although both Spc1p and Spc2p are noncatalytic, they are functionally distinct. Evidence is also presented that a double mutant lacking Spc1p and Spc2p grows well relative to wild type yeast cells, indicating that the signal peptidase complex missing at least two of its subunits is sufficient for signal peptidase activity in vivo.
|
['Amino Acid Sequence', 'Animals', 'Calcium-Calmodulin-Dependent Protein Kinases', 'Cloning, Molecular', 'Dogs', 'Fungal Proteins', 'Hot Temperature', 'Membrane Proteins', 'Mitogen-Activated Protein Kinases', 'Molecular Sequence Data', 'Mutation', 'Protein Precursors', 'Saccharomyces cerevisiae', 'Saccharomyces cerevisiae Proteins', 'Schizosaccharomyces pombe Proteins', 'Sequence Homology, Amino Acid', 'Serine Endopeptidases']
| 8,910,564
|
[['G02.111.570.060', 'L01.453.245.667.060'], ['B01.050'], ['D08.811.913.696.620.682.700.125', 'D12.644.360.100', 'D12.776.476.100'], ['E05.393.220'], ['B01.050.150.900.649.313.750.250.216.200'], ['D12.776.354'], ['G01.906.595.543', 'G16.500.275.063.725.710.380', 'G16.500.750.775.710.380', 'N06.230.300.100.725.232', 'N06.230.300.100.725.710.380'], ['D12.776.543'], ['D08.811.913.696.620.682.700.567', 'D12.644.360.450', 'D12.776.476.450'], ['L01.453.245.667'], ['G05.365.590'], ['D12.776.811'], ['B01.300.107.795.785.800', 'B01.300.930.705.655'], ['D12.776.354.750'], ['D12.776.354.875'], ['G02.111.810.200', 'G05.810.200'], ['D08.811.277.656.300.760', 'D08.811.277.656.959.350']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 1
| 0
|
Malinowski goes to college: factors influencing students' use of ritual and superstition.
|
Anthropologists have long noted that the use of ritual and magic is linked to conditions of risk and uncertainty. In this study, the authors examined how perceived task difficulty, participants' level of preparation, and the value of the outcome interact to influence the self-reporting of superstition and ritual. College students rated the likelihood of their using charms or rituals for various scenarios involving academic, artistic, and athletic performances. Reports of use of ritual increased as the stakes of the event increased and decreased with perceived expertise or level of preparation. Additional findings included participants' reporting frequent use of ritual while denying any causal effectiveness. The authors discuss results in terms of the rituals providing participants with an illusion of control.
|
['Adolescent', 'Adult', 'Anthropology', 'Ceremonial Behavior', 'Female', 'Humans', 'Male', 'Psychological Theory', 'Students', 'Superstitions', 'Teaching', 'Universities']
| 18,183,736
|
[['M01.060.057'], ['M01.060.116'], ['F04.096.879.201', 'I01.076'], ['F01.145.813.097', 'I01.076.201.450.170'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F02.739'], ['M01.848'], ['I01.076.201.450.897'], ['I02.903'], ['I02.783.830', 'J03.832.830']]
|
['Named Groups [M]', 'Psychiatry and Psychology [F]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Organisms [B]', 'Technology, Industry, and Agriculture [J]']
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
|
Citrobacter rodentium, the causative agent of transmissible murine colonic hyperplasia, exhibits clonality: synonymy of C. rodentium and mouse-pathogenic Escherichia coli.
|
Citrobacter rodentium (formerly Citrobacter freundii biotype 4280 and Citrobacter genomospecies 9) was described on the basis of biochemical characterization and DNA-DNA hybridization data and is the only Citrobacter species known to possess virulence factors homologous to those of the human pathogens enteropathogenic Escherichia coli and enterohemorrhagic E. coli. These virulence factors are encoded on the locus of enterocyte effacement (LEE), a pathogenicity island required for the characteristic attaching and effacing (AE) pathology seen in infection with these three pathogens. C. rodentium, which apparently infects only mice, provides a useful animal model for studying the molecular basis of AE pathology. No work has been done to assess differences in pathogenicity between C. rodentium isolates from diverse sources. Here, we report the examination of 15 C. rodentium isolates using a battery of genetic and biochemical approaches. No differences were observed between the isolates by repetitive-element sequence-based PCR analysis, biochemical analysis, and possession of LEE-specific virulence factors. These data suggest that members of the species are clonal. We further characterized an atypical E. coli strain from Japan called mouse-pathogenic E. coli (MPEC) that, in our hands, caused the same disease as C. rodentium. Applying the same battery of tests, we found that MPEC possesses LEE-encoded virulence factors and is indistinguishable from the previously characterized C. rodentium isolate DBS100. These results demonstrate that MPEC is a misclassified C. rodentium isolate and that members of this species are clonal and represent the only known attaching and effacing bacterial pathogen of mice.
|
['Animals', 'Chromosome Mapping', 'Citrobacter freundii', 'Colonic Diseases', 'Escherichia coli', 'Female', 'Hyperplasia', 'Male', 'Mice', 'Polymerase Chain Reaction', 'Rodent Diseases', 'Virulence']
| 11,101,562
|
[['B01.050'], ['E05.393.183'], ['B03.440.450.425.200.275', 'B03.660.250.150.100.210'], ['C06.405.469.158'], ['B03.440.450.425.325.300', 'B03.660.250.150.180.100'], ['C23.550.444'], ['B01.050.150.900.649.313.992.635.505.500'], ['E05.393.620.500'], ['C22.795'], ['G06.930']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Prolongation of the prothrombin time after organophosphate poisoning.
|
Prolongation of the prothrombin time owing to a transient reduction in factor VII activity is described in a 14-month-old child with organophosphate poisoning. Correction after vitamin K administration suggested an organophosphate-related effect on vitamin K-dependent factor VII activity. Historically, coagulation screening has not been routinely recommended after organophosphate intoxications. We suggest, however, that routine screening in such patients may be important. A brief review of organophosphate poisoning and the unique features of our case are presented.
|
['Acetylcholinesterase', 'Factor VII', 'Female', 'Humans', 'Infant', 'Organophosphate Poisoning', 'Poisoning', 'Prothrombin Time', 'Vitamin K']
| 7,845,858
|
[['D08.811.277.352.100.170.176'], ['D08.622.432', 'D12.776.124.125.325', 'D12.776.811.243.432', 'D23.119.325'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703'], ['C25.723.717'], ['C25.723'], ['E01.370.225.625.115.610', 'E05.200.625.115.610', 'G09.188.680'], ['D02.455.426.559.847.638.721.374', 'D02.455.849.291.523.500', 'D04.615.638.721.374']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Named Groups [M]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Dexamethasone, cerebrospinal fluid matrix metalloproteinase concentrations and clinical outcomes in tuberculous meningitis.
|
BACKGROUND: Adjunctive dexamethasone reduces mortality from tuberculous meningitis, but how it produces this effect is not known. Matrix metalloproteinases (MMPs) are important in the immunopathology of many inflammatory CNS diseases thus we hypothesized that that their secretion is important in TBM and might be influenced by dexamethasone.METHODOLOGY/PRINCIPAL FINDINGS: The kinetics of cerebrospinal fluid (CSF) MMP and tissue inhibitors of MMPs (TIMPs) concentrations were studied in a subset of HIV uninfected adults (n = 37) with TBM recruited to a randomized, placebo-controlled trial of adjuvant dexamethasone. Analysis followed a pre-defined plan. Dexamethasone significantly reduced CSF MMP-9 concentrations in early follow up samples (median 5 days (range 3-8) of treatment), but had no significant influence on other MMPs/TIMPs. Additionally CSF MMP-9 concentration was strongly correlated to concomitant CSF neutrophil count.CONCLUSIONS/SIGNIFICANCE: Dexamethasone decreased CSF MMP-9 concentrations early in treatment and this may represent one mechanism by which corticosteroids improve outcome in TBM. The strong correlation between CSF MMP-9 and neutrophil count suggests that polymorphonuclear leukocytes may play a central role in the early pathogenesis of TBM.
|
['Adult', 'Cerebrospinal Fluid', 'Chemotherapy, Adjuvant', 'Dexamethasone', 'Female', 'Glucocorticoids', 'Humans', 'Kinetics', 'Male', 'Matrix Metalloproteinase 9', 'Matrix Metalloproteinases', 'Middle Aged', 'Neutrophils', 'Placebos', 'Treatment Outcome', 'Tuberculosis, Meningeal']
| 19,789,647
|
[['M01.060.116'], ['A12.207.270.210'], ['E02.186.170', 'E02.319.170'], ['D04.210.500.745.432.769.344', 'D04.210.500.908.238'], ['D06.472.040.543', 'D27.505.696.399.472.488'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G01.374.661', 'G02.111.490'], ['D08.811.277.656.300.480.205.360', 'D08.811.277.656.300.480.252.445', 'D08.811.277.656.300.480.525.700.350', 'D08.811.277.656.675.374.205.360', 'D08.811.277.656.675.374.252.445', 'D08.811.277.656.675.374.525.700.350', 'D12.644.276.848.350', 'D12.776.467.836.350'], ['D08.811.277.656.300.480.525', 'D08.811.277.656.675.374.525'], ['M01.060.116.630'], ['A11.118.637.415.583', 'A11.627.340.583', 'A11.733.689', 'A15.145.229.637.415.583', 'A15.382.490.315.583', 'A15.382.680.689'], ['D26.660', 'E02.785'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['C01.150.252.223.500.937', 'C01.150.252.223.850.800', 'C01.150.252.410.040.552.846.570.600', 'C01.207.180.500.937', 'C01.207.180.850.800', 'C10.228.228.180.500.937', 'C10.228.228.180.850.800', 'C10.228.614.280.915']]
|
['Named Groups [M]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Fully intact contact heat evoked potentials in patients with amyotrophic lateral sclerosis.
|
Amyotrophic lateral sclerosis (ALS) is typically considered to be a disease of motor, not sensory, neurons. However, reports exist of sensory system involvement in ALS. In this study we aimed to study the characteristic of contact heat-evoked potentials (CHEPs) in patients with ALS and to evaluate the nociceptive pathway in these patients. Sixty patients with ALS and 60 controls had pain elicited by a CHEP stimulator with an accelerated velocity of 70 degrees C/s. Thermal stimuli were sent at 54.5 degrees C to three body sites: the dorsum of the hand, the proximal volar forearm, and the skin near C7. CHEPs were recorded from Cz and Pz. The onset negative peak latencies were 561.2 +/- 28.6 ms, 540.1 +/- 39.2 ms, and 502.4 +/- 26.2 ms when the dorsum of the hand, the proximal volar forearm, and skin near C7 were stimulated, respectively. There were no significant differences between the ALS patients and the controls with CHEP (P > 0.05). Our results suggest that the nociceptive pathway is intact and support the idea that small fibers and their sensory pathway are spared in ALS.
|
['Adult', 'Afferent Pathways', 'Aged', 'Amyotrophic Lateral Sclerosis', 'Evoked Potentials, Somatosensory', 'Female', 'Humans', 'Male', 'Middle Aged', 'Nerve Fibers, Unmyelinated', 'Neural Conduction', 'Nociceptors', 'Pain', 'Pain Measurement', 'Physical Stimulation', 'Reaction Time', 'Sensation Disorders', 'Sensory Receptor Cells', 'Thermosensing']
| 19,260,053
|
[['M01.060.116'], ['A08.612.220'], ['M01.060.116.100'], ['C10.228.854.139', 'C10.574.562.250', 'C10.574.950.050', 'C10.668.467.250', 'C18.452.845.800.050'], ['G07.265.216.500.400', 'G11.561.200.500.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['A08.675.542.756', 'A11.671.501.756'], ['G07.265.753', 'G11.561.601'], ['A08.675.650.915.875', 'A08.800.950.875', 'A11.671.650.915.875'], ['C23.888.592.612', 'F02.830.816.444', 'G11.561.790.444'], ['E01.370.600.550.324'], ['E05.723'], ['E05.796.817', 'F02.830.650', 'F04.669.817', 'G11.561.677'], ['C10.597.751', 'C23.888.592.763'], ['A08.675.650.915', 'A08.800.950', 'A11.671.650.915'], ['F02.830.816.781', 'G07.850', 'G11.561.790.781']]
|
['Named Groups [M]', 'Anatomy [A]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Psychiatry and Psychology [F]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 1
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Chemical studies on the structure of mucopeptide isolated from Streptococcus bovis.
|
Mucopeptides isolated from Streptococcus bovis cell walls were found to be composed of alanine, glutamic acid, lysine, and threonine in a mole ratio of 3:1:1:1. A dipeptide, N(epsilon)-lysylthreonine, was isolated from S. bovis mucopeptide by ion-exchange chromatography. This finding suggests that threonine is associated with the bridge which cross-links adjacent tetrapeptides by connecting the epsilon-amino group of lysine of one tetrapeptide to the carboxyl group of d-alanine of another to form the mucopeptide matrix.
|
['Alanine', 'Cell Wall', 'Chromatography, Ion Exchange', 'Glucosamine', 'Glutamates', 'Lysine', 'Mucoproteins', 'Streptococcus', 'Threonine']
| 5,802,603
|
[['D12.125.042'], ['A11.284.183'], ['E05.196.181.400.383'], ['D09.067.342.531'], ['D12.125.067.625', 'D12.125.119.409'], ['D12.125.068.555', 'D12.125.095.647', 'D12.125.142.497'], ['D12.776.395.560'], ['B03.353.750.737.872', 'B03.510.400.800.872', 'B03.510.550.737.872'], ['D12.125.142.815', 'D12.125.154.900']]
|
['Chemicals and Drugs [D]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]']
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Three-dimensional CT modeling versus traditional radiology techniques in treatment of acetabular fractures.
|
Recently, the authors have used the computer-generated three-dimensional (3-D) CT moving images for preoperative planning and screw/pin insertion in more than 28 cases involving plate and screw fixation of complex acetabular fractures. The authors also used stereolithography (wax or plastic 3-D model of bony anatomy) to develop a computer-generated "clip on" interpositioning template for accurate placement of plate and screws. Application of these new technologies gives the surgeon precise information about the fracture patterns and provides an effective means for preoperative planning and accurate fixation of acetabular fractures. The accuracy of the 3-D virtual presentation of the anatomy is impressive and was substantiated by phantom studies. Postoperation CT revealed no case of screw penetration in the joint. Among other benefits over conventional surgical technique, the computer-assisted surgery provided decreased operative time and morbidity, decreased radiation exposure, and obviated the need for oblique, inlet and outlet roentgen views of the pelvis for preoperative planning. A case report specifically demonstrates preoperative planning for reconstruction of acetabular fracture.
|
['Acetabulum', 'Adult', 'Fractures, Bone', 'Humans', 'Imaging, Three-Dimensional', 'Male', 'Tomography, X-Ray Computed']
| 11,813,946
|
[['A02.835.232.043.825.108'], ['M01.060.116'], ['C26.404'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.350.400', 'L01.224.308.410'], ['E01.370.350.350.810', 'E01.370.350.600.350.700.810', 'E01.370.350.700.700.810', 'E01.370.350.700.810.810', 'E01.370.350.825.810.810']]
|
['Anatomy [A]', 'Named Groups [M]', 'Diseases [C]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Information Science [L]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
| 0
|
Early pregnancy testing and its relationship to abortion.
|
A total of 487 Standardbred and Thoroughbred mares at two studs were manually tested for pregnancy at 20 to 24, 30 to 34, and greater than 42 days after service and the abortion rate compared to that obtained in previous years when only the greater than 42-day test was performed. The results indicated that early manual pregnancy testing does not increase the abortion rate if undertaken carefully and enables non-pregnant mares to be re-mated earlier in the same season.
|
['Abortion, Veterinary', 'Animals', 'Australia', 'Female', 'Horses', 'Palpation', 'Pregnancy', 'Pregnancy Tests', 'Pregnancy, Animal', 'Time Factors']
| 1,060,828
|
[['C13.703.039.422', 'C22.021'], ['B01.050'], ['Z01.639.100', 'Z01.678.100.373'], ['B01.050.150.900.649.313.984.235.472'], ['E01.370.600.600'], ['G08.686.784.769'], ['E01.370.225.970', 'E01.370.378.620', 'E05.200.970'], ['G08.686.784.769.498'], ['G01.910.857']]
|
['Diseases [C]', 'Organisms [B]', 'Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
|
Macrophages as delivery vehicles for anticancer agents.
|
The delivery of anticancer agents via passive approaches such as the enhanced permeability and retention effect is unlikely to achieve sufficient concentrations throughout the tumor volume for effective treatment. Cell-based delivery approaches using tumor tropic cells have the potential to overcome the limitations of passive approaches. Specifically, this review focuses on the use of monocytes/macrophages for the delivery of a variety of anticancer agents, including nanoparticles, chemotherapeutics and gene constructs. The efficacy of this delivery approach, both as monotherapy and in combination with light-based phototherapy modalities, has been demonstrated in numerous in vitro and animal studies, however, its clinical potential remains to be determined.
|
['Animals', 'Antigen-Presenting Cells', 'Antineoplastic Agents', 'Cytosine Deaminase', 'Humans', 'Macrophages', 'Nanoparticles', 'Neoplasms', 'Photosensitizing Agents', 'Phototherapy']
| 30,909,858
|
[['B01.050'], ['A11.066', 'A15.382.066'], ['D27.505.954.248'], ['D08.811.277.151.486.625'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A11.329.372', 'A11.627.482', 'A11.733.397', 'A15.382.670.522', 'A15.382.680.397'], ['J01.637.512.600'], ['C04'], ['D27.505.954.444.600', 'D27.505.954.600.710'], ['E02.774']]
|
['Organisms [B]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Technology, Industry, and Agriculture [J]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Smoking Lung Cancer Patients and Tobacco Cessation - Is the Current Treatment in Germany Sufficient?
|
Lung cancer is the most preventable neoplastic disease for men and women. The incidence rate per year is 14.000 in Germany. Smoking is the main risk factor for the onset of lung cancer and for a share of 90% of cases, lung cancer is associated with smoking. Recent studies have shown that the time slot of diagnosing lung cancer is a teachable moment for tobacco cessation interventions. The therapy that was rated most effective was a combination of cognitive behavioral therapy and pharmacotherapy (e. g. NRT, Bupropion, Varenicline). We examined the smoking status of all patients undergoing lung cancer surgery in 2011, 2012 and 2013 in this study. A retrospective semi structured interview via telephone was conducted regarding smoking habits and current quality of life. 131 patients (36.6% female, average age of 68.7 years) of an urban German hospital were included.Results showed a relapse rate of 22.3%, while 86.2% used to be highly addicted smokers; A multivariate analysis of covariance (MANCOVA) indicated a significant overall impact of smoking status on quality of life with a medium effect size, controlled for age, gender, living conditions, tumor stage, duration of smoking abstinence, type of cancer therapy, type of resection method, and the time period between the date of surgery and of the survey. Two thirds of all smokers did not see an association between their habit and their disease.So far motivation to quit and long term abstinence rates are not sufficiently established even among seriously sick patients in Germany; further initiatives should focus on new and more intense interventions and educational strategies.
|
['Adolescent', 'Adult', 'Age Distribution', 'Aged', 'Comorbidity', 'Female', 'Germany', 'Health Care Surveys', 'Humans', 'Lung Neoplasms', 'Male', 'Middle Aged', 'Prevalence', 'Quality of Life', 'Retrospective Studies', 'Risk Factors', 'Sex Distribution', 'Smoking', 'Smoking Prevention', 'Tobacco Use Cessation', 'Treatment Outcome', 'Utilization Review', 'Young Adult']
| 26,398,407
|
[['M01.060.057'], ['M01.060.116'], ['I01.240.050', 'N01.224.033', 'N06.850.505.400.050'], ['M01.060.116.100'], ['N05.715.350.225', 'N06.850.490.687'], ['Z01.542.315'], ['E05.318.308.980.344', 'N03.349.380.210', 'N05.425.210', 'N05.715.360.300.800.344', 'N06.850.520.308.980.344'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C04.588.894.797.520', 'C08.381.540', 'C08.785.520'], ['M01.060.116.630'], ['E05.318.308.985.525.750', 'N01.224.935.597.750', 'N06.850.505.400.975.525.750', 'N06.850.520.308.985.525.750'], ['I01.800', 'K01.752.400.750', 'N06.850.505.400.425.837'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['I01.240.800', 'N01.224.803', 'N06.850.505.400.850'], ['F01.145.805'], ['I02.233.332.812', 'N02.421.726.407.840'], ['F01.145.488.750'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['N04.761.879', 'N05.700.900'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]', 'Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Diseases [C]', 'Humanities [K]', 'Psychiatry and Psychology [F]']
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 1
|
Lipid and lipoprotein parameters for detection of familial hypercholesterolemia in childhood. The DECOPIN Project.
|
BACKGROUND: Familial hypercholesterolaemia (FH) in children is under-detected and is difficult to diagnose in clinical practice. The aim of this study was to evaluate clinical, biochemical and vascular imaging variables in order to detect children and adolescents with FH.METHODS: A total of 222 children aged 4-18 years old were recruited to participate in a project for the early detection of FH (The DECOPIN Project). They were distributed into 3groups: FH, if genetic study or clinical criteria were positive (n=91); Polygenic hypercholesterolaemia (PH) if LDL-Cholesterol >135mg/dL without FH criteria (n=23), and Control group (CG) if LDL-C <135mg/dL (n=108). Data were collected from family history, anthropometric data, and clinical variables. The usual biochemical parameters, including a complete lipid profile were analysed. The carotid intima-media thickness (cIMT) and thickness of Achilles tendons were determined using ultrasound in all participants.RESULTS: A total of 91 children had a diagnosis of FH, 23 with PH, and 108 with CG. Children with FH had higher concentrations of total cholesterol, LDL-C, ApoB/ApoA1 ratio, and cholesterol-year score, than the other groups. HDL-C was lower in the FH group than in the CG. Thickness of the Achilles tendon and cIMT did not show any differences between groups, although a greater cIMT trend was observed in the FH group. ApoB/ApoA1 ratio >0.82 was the parameter with the highest sensitivity and specificity to predict the presence of mutation in children with FH.CONCLUSIONS: Although LDL-C is the main biochemical parameter used to define FH, the ApoB/ApoA1 ratio (>0.82) may be a useful tool to identify children with FH and a positive mutation.
|
['Achilles Tendon', 'Adolescent', 'Apolipoprotein A-I', 'Apolipoprotein B-100', 'Carotid Intima-Media Thickness', 'Case-Control Studies', 'Child', 'Child, Preschool', 'Cholesterol, HDL', 'Cholesterol, LDL', 'Female', 'Humans', 'Hypercholesterolemia', 'Hyperlipoproteinemia Type II', 'Lipids', 'Male', 'Mutation', 'Sensitivity and Specificity']
| 29,602,595
|
[['A02.880.176'], ['M01.060.057'], ['D10.532.091.200.100', 'D12.776.070.400.200.100', 'D12.776.521.120.200.100'], ['D10.532.091.300.249', 'D12.776.070.400.300.249', 'D12.776.521.120.300.249'], ['E01.370.350.850.150', 'E01.370.370.180', 'G09.330.210'], ['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['M01.060.406'], ['M01.060.406.448'], ['D04.210.500.247.808.197.238', 'D10.532.432.400', 'D10.570.938.208.270', 'D12.776.521.479.470'], ['D04.210.500.247.808.197.244', 'D10.532.515.500', 'D10.570.938.208.275', 'D12.776.521.550.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C18.452.584.500.500.396'], ['C16.320.565.398.481', 'C18.452.584.500.500.644.475', 'C18.452.648.398.481'], ['D10'], ['G05.365.590'], ['E05.318.370.800', 'E05.318.740.872', 'G17.800', 'N05.715.360.325.700', 'N05.715.360.750.725', 'N06.850.520.445.800', 'N06.850.520.830.872']]
|
['Anatomy [A]', 'Named Groups [M]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Organisms [B]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Preparation of folate-modified pullulan acetate nanoparticles for tumor-targeted drug delivery.
|
The purpose of this work was to develop a novel nano-carrier with targeting property to tumor. In this study, pullulan acetate (PA) was synthesized by the acetylation of pullulan to simplify the preparation technique of nanoparticles. Folic acid (FA) was conjugated to PA in order to improve the cancer-targeting activity. The products were characterized by proton nuclear magnetic resonance (?H NMR) spectroscopy. Epirubicin-loaded nanoparticles were prepared by a solvent diffusion method. The loading efficiencies and EPI content increased with the amount of triethylamine (TEA) increasing in some degree. FPA nanoparticles could incorporate more epirubicin than PA nanoparticles. The folate-modified PA nanoparticles (FPA/EPI NPs) exhibited faster drug release than PA nanoparticles (PA/EPI NPs) in vitro. Confocal image analysis and flow cytometry test revealed that FPA/EPI NPs exhibited a greater extent of cellular uptake than PA/EPI NPs against KB cells over-expressing folate receptors on the surface. FPA/EPI NPs also showed higher cytotoxicity than PA/EPI NPs. The cytotoxic effect of FPA/EPI NPs to KB cells was inhibited by an excess amount of folic acid, suggesting that the binding and/or uptake were mediated by the folate receptor.
|
['Acetylation', 'Antibiotics, Antineoplastic', 'Cell Culture Techniques', 'Cell Survival', 'Drug Carriers', 'Drug Compounding', 'Endocytosis', 'Epirubicin', 'Flow Cytometry', 'Folic Acid', 'Glucans', 'Humans', 'KB Cells', 'Microscopy, Confocal', 'Microscopy, Electron, Transmission', 'Nanoparticles', 'Particle Size', 'Solubility', 'Surface Properties']
| 22,747,075
|
[['G02.111.012.052', 'G02.607.063.052', 'G03.040.052'], ['D27.505.954.248.106'], ['E01.370.225.500.223', 'E05.200.500.265', 'E05.242.223', 'E05.481.500.249'], ['G04.346'], ['D26.255.260', 'E02.319.300.380'], ['E05.916.270'], ['G04.417'], ['D02.455.426.559.847.562.050.200.175.200', 'D04.615.562.050.200.175.200', 'D09.408.051.059.200.175.200'], ['E01.370.225.500.363.342', 'E01.370.225.500.386.350', 'E05.196.712.516.600.240.350', 'E05.200.500.363.342', 'E05.200.500.386.350', 'E05.242.363.342', 'E05.242.386.350'], ['D03.633.100.733.631.400'], ['D05.750.078.562', 'D09.698.365'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A11.251.210.190.400.500', 'A11.251.860.180.400.500', 'A11.436.340.500'], ['E01.370.350.515.395', 'E05.595.395'], ['E01.370.350.515.402.580', 'E05.595.402.580'], ['J01.637.512.600'], ['G02.712'], ['G02.805'], ['G02.860']]
|
['Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Anatomy [A]', 'Technology, Industry, and Agriculture [J]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Fashion advertisements: a comparison of viewers' perceptual and affective responses to illustrated and photographed stimuli.
|
The perceptual and affective responses of 44 women to actual illustrated and photographed fashion advertisements during focused interviews were explored. Content analysis methods identified categories of response; frequency of response categories for the two media were compared using Fisher's z tests. Significant differences in perceptual responses included greater visual interest created by the use of color in photographs, greater interest in layout and design features of the illustrations, and interest in characteristics of the models in the photographs. Affective response differences included greater preference for photographic advertisements and the garments in them. Contrary to suggestions from professionals in fashion advertising, no significant differences were found in viewers' perceptions of information about the products in the advertisements or perceptions of meaning and aesthetic response.
|
['Adult', 'Advertising', 'Affect', 'Aged', 'Art', 'Female', 'Humans', 'Middle Aged', 'Perception', 'Periodicals as Topic', 'Photography']
| 1,484,770
|
[['M01.060.116'], ['J01.219.687.274', 'L01.143.050'], ['F01.470.047'], ['M01.060.116.100'], ['K01.093'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['F02.463.593'], ['L01.178.682.829.678'], ['E01.370.350.600', 'E05.712']]
|
['Named Groups [M]', 'Technology, Industry, and Agriculture [J]', 'Information Science [L]', 'Psychiatry and Psychology [F]', 'Humanities [K]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 1
| 1
| 1
| 0
| 0
|
[Hypotension in Austria (author's transl)].
|
A representative group of the Austrian population was surveyed to gain informations about various aspects of the blood pressure problem. 22% of the persons surveyed (16-70 years) reported hypotension and 14% hypertension. Especially women very often (29%) consider their blood pressure rather low. 17% of the respondents consider hypotension "very dangerous", 47% "dangerous under certain conditions"; 30% prefer the statement "rather not dangerous". Women are less concerned about hypotension than men. Although hypotension very often is not recognized as a serious health problem this issue has implication on morbidity statistics and other aspects of public health, e. g. frequency of medical consultations, drug treatment, health economy. The population shows a high degree of awareness of this question of health.
|
['Adolescent', 'Adult', 'Age Factors', 'Aged', 'Attitude to Health', 'Austria', 'Female', 'Humans', 'Hypertension', 'Hypotension', 'Male', 'Middle Aged', 'Sex Factors']
| 7,257,424
|
[['M01.060.057'], ['M01.060.116'], ['N05.715.350.075', 'N06.850.490.250'], ['M01.060.116.100'], ['F01.100.150', 'N05.300.150'], ['Z01.542.088'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C14.907.489'], ['C14.907.514'], ['M01.060.116.630'], ['N05.715.350.675', 'N06.850.490.875']]
|
['Named Groups [M]', 'Health Care [N]', 'Psychiatry and Psychology [F]', 'Geographicals [Z]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
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