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A PLCdelta1-binding protein, p122RhoGAP, is localized in focal adhesions.
|
We have investigated the cellular distribution of p122RhoGAP, a GTPase-activating protein of Rho small GTPase and an activator of phospholipase C-delta(1). Immunofluorescence studies demonstrated that endogenous p122 is localized at the tips of actin stress fibres and co-localizes with vinculin in normal rat kidney cells. In immunoprecipitation studies, p122 co-precipitated with vinculin, indicating that p122 is localized at the sites of focal adhesion. We have also shown that the N-terminal half of p122 is responsible for this localization. It is conceivable, therefore, that p122 is involved in the reorganization of the actin cytoskeleton and focal adhesions that regulate cell-substratum adhesion and cell migration.
|
['Animals', 'Binding Sites', 'Cell Adhesion', 'GTPase-Activating Proteins', 'Isoenzymes', 'Kidney', 'Models, Biological', 'Phospholipase C delta', 'Protein Binding', 'Rats', 'Type C Phospholipases', 'Vinculin']
| 15,506,980
|
[['B01.050'], ['G02.111.570.120'], ['G04.022'], ['D12.644.360.325.150', 'D12.776.476.325.150'], ['D08.811.348', 'D12.776.800.300'], ['A05.810.453'], ['E05.599.395'], ['D08.811.277.352.640.700.700.562.625', 'D12.644.360.571.625', 'D12.776.476.556.625'], ['G02.111.679', 'G03.808'], ['B01.050.150.900.649.313.992.635.505.700'], ['D08.811.277.352.640.700.700'], ['D12.776.220.990']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
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| 0
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| 0
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| 0
|
The oxidative refolding of hen lysozyme and its catalysis by protein disulfide isomerase.
|
The oxidative refolding of hen lysozyme has been studied by a variety of time-resolved biophysical methods in conjunction with analysis of folding intermediates using reverse-phase HPLC. In order to achieve this, refolding conditions were designed to reduce aggregation during the early stages of the folding reaction. A complex ensemble of relatively unstructured intermediates with on average two disulfide bonds is formed rapidly from the fully reduced protein after initiation of folding. Following structural collapse, the majority of molecules slowly form the four-disulfide-containing fully native protein via rearrangement of a highly native-like, kinetically trapped intermediate, des-[76-94], although a significant population (approximately 30%) appears to fold more quickly via other three-disulfide intermediates. The folding catalyst PDI increases dramatically both yields and rates of lysozyme refolding, largely by facilitating the conversion of des-[76-94] to the native state. This suggests that acceleration of the folding rate may be an important factor in avoiding aggregation in the intracellular environment.
|
['Animals', 'Catalysis', 'Chickens', 'Chromatography, High Pressure Liquid', 'Circular Dichroism', 'Disulfides', 'Kinetics', 'Magnetic Resonance Spectroscopy', 'Models, Chemical', 'Models, Molecular', 'Muramidase', 'Oxidation-Reduction', 'Protein Disulfide-Isomerases', 'Protein Folding', 'Spectrometry, Fluorescence', 'Time Factors']
| 10,469,657
|
[['B01.050'], ['G02.130'], ['B01.050.150.900.248.350.150', 'B01.050.150.900.248.690.192'], ['E05.196.181.400.300'], ['E05.196.867.151'], ['D01.248.497.158.874.390', 'D01.875.350.850.150', 'D02.886.520.150'], ['G01.374.661', 'G02.111.490'], ['E05.196.867.519'], ['E05.599.495'], ['E05.599.595'], ['D08.811.277.450.642'], ['G02.700', 'G03.295.531'], ['D08.811.399.475.800.550'], ['G01.154.651', 'G02.111.688'], ['E05.196.712.516.600.676', 'E05.196.867.726'], ['G01.910.857']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Correlated slow fluctuations in respiration, EEG, and BOLD fMRI.
|
Low-frequency temporal fluctuations of physiological signals (<0.1 Hz), such as the respiration and cardiac pulse rate, occur naturally during rest and have been shown to be correlated with blood-oxygenation-level-dependent (BOLD) signal fluctuation. Such physiological signal modulations have been considered as sources of noise and their effects on BOLD signal are commonly removed in functional magnetic resonance imaging (fMRI) studies. However, possible neural correlates of the physiological fluctuations have not been considered nor examined in detail. In the present study we investigated this possibility by simultaneously acquiring electroencephalogram (EEG) with BOLD fMRI data, respiratory and cardiac waveforms in healthy human subjects at eyes-closed and eyes-open resting. We quantified the concurrent changes of the EEG power in the alpha frequency band, the respiration volume, and the cardiac pulse rate, then assessed the temporal correlations between alpha EEG power and physiological signal fluctuations. In addition, time-shifted time courses of alpha EEG power or physiological data were included as regressors to examine their correlations with the whole-brain BOLD fMRI signals. We observed a significant correlation between alpha EEG global field power and respiration, particularly at eyes-closed resting condition. Similar spatial patterns were observed between the correlation maps of BOLD with alpha EEG power and respiration, with negative correlations coinciding in the visual cortex, superior/middle temporal gyrus, inferior frontal gyrus, and inferior parietal lobule and positive correlations in the thalamus and caudate. Regressing out the physiological variations in the BOLD signal resulted in reduced correlation between BOLD and alpha EEG power. These results suggest a mutual link of neuronal origin between alpha EEG power, respiration, and BOLD signals.
|
['Adult', 'Biological Clocks', 'Brain', 'Brain Mapping', 'Electroencephalography', 'Female', 'Heart Rate', 'Humans', 'Magnetic Resonance Imaging', 'Male', 'Reproducibility of Results', 'Respiratory Rate', 'Sensitivity and Specificity', 'Statistics as Topic']
| 23,631,982
|
[['M01.060.116'], ['G07.180.562.094'], ['A08.186.211'], ['E01.370.350.578.875.500', 'E01.370.376.537.625.500', 'E05.629.875.500'], ['E01.370.376.300', 'E01.370.405.245'], ['E01.370.600.875.500', 'G09.330.380.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.350.825.500'], ['E05.318.370.725', 'E05.337.851', 'N05.715.360.325.685', 'N06.850.520.445.725'], ['E01.370.600.875.875', 'G09.772.705.730'], ['E05.318.370.800', 'E05.318.740.872', 'G17.800', 'N05.715.360.325.700', 'N05.715.360.750.725', 'N06.850.520.445.800', 'N06.850.520.830.872'], ['E05.318.740', 'H01.548.832', 'N05.715.360.750', 'N06.850.520.830']]
|
['Named Groups [M]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Health Care [N]', 'Disciplines and Occupations [H]']
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 1
| 1
| 0
|
Piagetian conservation and response to cognitive therapy in attention deficit disordered children.
|
Cognitive therapy is often used in treating attention-deficit-disordered (ADD) children because of its purported ability to address this population's attentional deficits and behavioral difficulties and to create durable therapeutic effects. Nonetheless, research findings on these treatments have been inconsistent. This study sought to explain these inconsistencies by examining the influence of cognitive development on children's ability to benefit from such treatment. The ability to conserve number and substance was shown to influence treatment outcome, as measured by laboratory tasks of cognitive style and impulsivity. No effects were found on standardized academic achievement measures or on behavioral ratings by teachers or parents. Further study of individual development will be important in understanding the outcomes of cognitive therapy.
|
['Attention', 'Attention Deficit Disorder with Hyperactivity', 'Child', 'Child Development', 'Cognition Disorders', 'Concept Formation', 'Education, Special', 'Female', 'Humans', 'Male', 'Problem Solving']
| 3,667,738
|
[['F02.830.104.214'], ['F03.625.094.150'], ['M01.060.406'], ['F01.525.200', 'G07.345.374.750'], ['F03.615.250'], ['F02.463.785.233'], ['I02.233.213'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F02.463.425.725', 'F02.463.785.810']]
|
['Psychiatry and Psychology [F]', 'Named Groups [M]', 'Phenomena and Processes [G]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Organisms [B]']
| 0
| 1
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
|
Protective Role of Glutathione against Peroxynitrite-Mediated DNA Damage During Acute Inflammation.
|
Inflammation is an immune response to protect against various types of infections. When unchecked, acute inflammation can be life-threatening, as seen with the current coronavirus pandemic. Strong oxidants, such as peroxynitrite produced by immune cells, are major mediators of the inflammation-associated pathogenesis. Cellular thiols play important roles in mitigating inflammation-associated macromolecular damage including DNA. Herein, we have demonstrated a role of glutathione (GSH) and other thiols in neutralizing the effect of peroxynitrite-mediated DNA damage through stable GSH-DNA adduct formation. Our observation supports the use of thiol supplements as a potential therapeutic strategy against severe COVID-19 cases and a Phase II (NCT04374461) open-label clinical trial launched in early May 2020 by the Memorial Sloan Kettering Cancer Center.
|
['Acute Disease', 'Animals', 'Betacoronavirus', 'COVID-19', 'Cattle', 'Coronavirus Infections', 'DNA', 'DNA Adducts', 'DNA Damage', 'Glutathione', 'HEK293 Cells', 'Humans', 'Inflammation', 'Mutagens', 'Pandemics', 'Peroxynitrous Acid', 'Pneumonia, Viral', 'SARS-CoV-2', 'Salmonella typhimurium']
| 32,894,672
|
[['C23.550.291.125'], ['B01.050'], ['B04.820.578.500.540.150.113'], ['C01.748.214', 'C01.748.610.763.500', 'C01.925.705.500', 'C01.925.782.600.550.200.163', 'C08.381.677.807.500', 'C08.730.214', 'C08.730.610.763.500'], ['B01.050.150.900.649.313.500.380.271'], ['C01.925.782.600.550.200'], ['D13.444.308'], ['D13.444.308.135', 'G05.200.104'], ['G05.200'], ['D12.644.456.448'], ['A11.251.210.172.750', 'A11.436.334'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C23.550.470'], ['D27.888.569.468'], ['N06.850.290.200.600'], ['D01.625.600.800', 'D01.625.700.750'], ['C01.748.610.763', 'C01.925.705', 'C08.381.677.807', 'C08.730.610.763'], ['B04.820.578.500.540.150.113.968'], ['B03.440.450.425.800.200.825', 'B03.660.250.150.710.160.760']]
|
['Diseases [C]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Health Care [N]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Structural changes of the carpal tunnel, median nerve and flexor tendons in MRI before and after endoscopic carpal tunnel release.
|
The purpose of this study is to investigate the structural changes of the carpal tunnel, median nerve, and flexor tendons in magnetic resonance imaging (MRI) before and after endoscopic carpal tunnel release (ECTR). We studied 36 hands undergoing ECTR. In MRI, the cross-sectional area of the carpal tunnel and the median nerve at the hamate and the pisiform levels were measured. The distance from the volar side of carpal bone to the median nerve or tendons and the volar displacement were measured. In post-operative MRI, the transverse carpal ligament could not be well delineated and the carpal tunnel was significantly enlarged both at the hamate and pisiform levels. The median nerve was enlarged at the hamate level. The median nerve and flexor tendons significantly moved to the volar side. The volar displacement of the median nerve and flexor digitorum superficialis in the long and ring fingers was greater than the other tendons.
|
['Aged', 'Aged, 80 and over', 'Carpal Tunnel Syndrome', 'Female', 'Humans', 'Magnetic Resonance Imaging', 'Male', 'Median Nerve', 'Middle Aged', 'Postoperative Care', 'Preoperative Care', 'Tendons', 'Wrist']
| 24,875,502
|
[['M01.060.116.100'], ['M01.060.116.100.080'], ['C10.668.829.500.500.200', 'C10.668.829.550.200', 'C26.844.150.206'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.350.825.500'], ['A08.800.800.720.050.500'], ['M01.060.116.630'], ['E02.760.731.700', 'E04.604.500', 'N02.421.585.722.700'], ['E02.760.795', 'E04.604.750', 'N02.421.585.795'], ['A02.880'], ['A01.378.800.875']]
|
['Named Groups [M]', 'Diseases [C]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Health Care [N]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
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| 0
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|
[Causes of inadequate vision in drivers and their significance in traffic].
|
From an analysis of the data of 462 automobile drivers with inadequate visual acuity it was established that the frequency of visits to the ophthalmologist by drivers with inadequate vision is determined less by age than by the cause of the visual deficiency. Here, considerable differences appear in the duration of inadequate vision before it is detected by the ophthalmologist and in the frequency of visits to the ophthalmologist. These have the effect that the refractive error, the most common visual deficiency among younger drivers, is considerably more important as an accident risk in road traffic than one might assume from the number of those affected. A precautionary elimination of visual deficiencies as cause of accidents therefore necessitates an examination of visual acuity which includes all age groups. In order to eliminate visual deficiencies in road traffic as a cause of accidents as a precaution, the officials responsible must find a practicable way of detecting and eliminating these deficiencies so early as possible. On the other hand, ophthalmologists must make it clear to their visually unsuited patients that in their own and the general interest they should give up driving.
|
['Adolescent', 'Adult', 'Age Factors', 'Aged', 'Automobile Driving', 'Cataract', 'Disability Evaluation', 'Female', 'Glaucoma', 'Humans', 'Macular Degeneration', 'Male', 'Middle Aged', 'Refractive Errors', 'Vision Disorders', 'Visual Acuity']
| 3,573,652
|
[['M01.060.057'], ['M01.060.116'], ['N05.715.350.075', 'N06.850.490.250'], ['M01.060.116.100'], ['I03.125'], ['C11.510.245'], ['E01.370.400'], ['C11.525.381'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C11.768.585.439'], ['M01.060.116.630'], ['C11.744'], ['C10.597.751.941', 'C11.966', 'C23.888.592.763.941'], ['E01.370.380.850.950', 'F02.463.593.932.901', 'G14.940']]
|
['Named Groups [M]', 'Health Care [N]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 0
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 0
|
Exogenous surfactant application in respiratory failure due to chronic obstructive pulmonary disease.
|
Recent results of basic research on regulation of surfactant secretion and surfactant physiology not only in the alveolus but also in peripheral small airways allow the conclusion that disorders in surfactant homeostasis may contribute to the pathophysiology of airway obstruction and hyperinflation. We therefore hypothesized that patients with respiratory failure due to obstructive lung disease may benefit from exogenous surfactant. Here we report a case that indicates the clinical situation to be considered for treatment with exogenous surfactant. The benefit for the patient was successful weaning from the ventilator. Improvements in effective compliance, resistance and blood gas parameters were observed following surfactant application.
|
['Aged', 'Biological Products', 'Female', 'Humans', 'Lung Diseases, Obstructive', 'Pulmonary Surfactants', 'Respiration', 'Respiratory Insufficiency', 'Smoking', 'Time Factors']
| 7,569,338
|
[['M01.060.116.100'], ['D20.215'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C08.381.495'], ['D27.505.954.796.600'], ['G09.772.705'], ['C08.618.846'], ['F01.145.805'], ['G01.910.857']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Psychiatry and Psychology [F]']
| 0
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Variations of Runoff and Sediment Load in the Middle and Lower Reaches of the Yangtze River, China (1950-2013).
|
On the basis of monthly runoff series obtained in 1950-2013 and annual sediment load measured in 1956--2013 at five key hydrological stations in the middle and lower reaches of the Yangtze River basin, this study used the Mann-Kendall methods to identify trend and abrupt changes of runoff and sediment load in relation to human activities. The results were as follows: (1) The annual and flood season runoffs showed significant decreasing trends at Yichang station, and showed slight downward trends at Hankou and Datong stations, while the abrupt changes of dry season runoff at Yichang, Hankou and Datong stations occurred in about 2007 and the change points were followed by significant increasing trends. The construction of the Three Gorges Dam, which began to operate in 2003, influenced the variations of runoff in the mainstream of Yangtze River, but the effect weakened with the distance along the downstream direction from TGD. (2) Since the 1990s, annual sediment loads at Yichang, Hankou, and Datong stations have been decreasing significantly, and after 2002, the annual sediment load at Yichang dropped below that of Hankou and Datong. The dams and deforestation/forestation contributed to the significant decreasing trend of the sediment load. In addition, the Three Gorges Dam aggravated the downward trend and caused the erosion of the riverbed and riverbanks in the middle and lower reaches. (3) The runoff and sediment load flowing from Dongting Lake into the mainstream of the Yangtze River showed significant decreasing trends at Chenglingji station after 1970s, and in contrast, slight increase in the sediment flow from Poyang Lake to the mainstream of the Yangtze River at Hukou station were detected over the post-TGD period (2003-2013). The result of the study will be an important foundation for watershed sustainable development of the Yangtze River under the human activities.
|
['Algorithms', 'China', 'Environmental Monitoring', 'Geologic Sediments', 'Rivers', 'Seasons']
| 27,479,591
|
[['G17.035', 'L01.224.050'], ['Z01.252.474.164'], ['N06.850.460.350.080', 'N06.850.780.375'], ['G01.311.330', 'G16.500.320'], ['G01.311.750', 'G16.500.275.280.650', 'N06.230.232.650'], ['G01.910.645.661', 'G16.500.275.071.590', 'N06.230.300.100.250.525']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Geographicals [Z]', 'Health Care [N]']
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 1
| 1
|
Scanning Electron Microscopy of Sand Flies of the Chagasi Series, Psychodopygus (Diptera: Psychodidae) Genus, Focusing on the Genitalia.
|
Males of cryptic or closely related species present great morphological variation in their genitalia, whereas females, such as those of the Chagasi Series of the Psychodopygus Mangabeira, 1941 genus, are more similar. Therefore, our aim was to study the fine structure of the male genitalia of five species of the Chagasi Series to better understand the variation in their morphology and its influence on the copulatory process. The sand fly species were captured in the following Brazilian states: Psychodopygus chagasi (Costa Lima, 1941) (Rond?nia), Psychodopygus complexus (Mangabeira, 1941) (Tocantins), Psychodopygus squamiventris maripaensis (Floch & Abonnenc, 1946) (Amap?), Psychodopygus squamiventris squamiventris (Lutz & Neiva, 1912) (Amazonas), and Psychodopygus wellcomei Fraiha, Shaw & Lainson, 1971 (Par? and Cear?). Insects were stored in ethanol 70% (then dehydrated) and dry after they were sputtered with gold. The samples were observed under a scanning electron microscope. Microtrichiae, two types of trichoid sensilla, coeloconic and chaetic sensillae, were observed on the antenna of all species, with no difference between them. Only on the anepimeron of P. squamiventris squamiventris a modified 'racket'-like scale was observed. As for the male genitalia, the setae and structures of each species were fully described, such as the small setae on the paramere apex of the P. squamiventris subspecies, and the grooves present in this region and on the paramere lobe of P. complexus and P. wellcomei, which are impossible to observe with optic microscopy. New information is thus provided on the male genitalia, which can contribute to future bionomic studies of these species.
|
['Animals', 'Brazil', 'Genitalia, Male', 'Insect Vectors', 'Leishmaniasis, Cutaneous', 'Male', 'Psychodidae']
| 31,576,409
|
[['B01.050'], ['Z01.107.757.176'], ['A05.360.444'], ['N06.850.335.188.100.500', 'N06.850.520.203.375.100.500'], ['C01.610.752.300.500.400', 'C01.610.858.560.400', 'C01.920.813.400', 'C17.800.838.775.560.400'], ['B01.050.500.131.617.720.500.500.750.781']]
|
['Organisms [B]', 'Geographicals [Z]', 'Anatomy [A]', 'Health Care [N]', 'Diseases [C]']
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
|
A simplified method for separate measurements of lung and chest wall mechanics in ventilator-treated patients.
|
BACKGROUND: Airway pressure measurements above the endotracheal tube will be distorted because of endotracheal tube resistance. To separate lung and chest wall compliance, esophageal pressure is conventionally measured with an air-filled balloon catheter, which is difficult to insert in unconscious patients. We have developed a methodology with fluid-filled catheters for intratracheal and esophageal pressure measurements.METHODS: Twelve anesthetized patients were studied. Tidal volumes were measured by side-stream spirometry. Airway pressures were measured at the Y-piece and in the trachea with fluid-filled pressure lines. Esophageal pressure was measured via the narrow lumen in a fluid-filled Salem double-lumen stomach tube, which was slowly retracted from the stomach up through the esophagus until maximal respiratory pressure readings and minimal cardiac artifacts were obtained. Lung mechanics were measured at different tidal volumes (TV) and positive end-expiratory pressure (PEEP).RESULTS: There was a significant difference between airway pressures at the Y-piece and in the trachea. Total compliance significantly increased with increasing TV and decreased with increasing PEEP. Chest wall compliance increased significantly with increasing TV, while lung compliance did not change significantly. Two patients showed repeatedly marked increase in lung compliance at one specific ventilatory setting, an increase the proportion of which was not reflected by changes in total compliance.CONCLUSIONS: Y-piece pressures are not representative of intratracheal pressures, which can be measured by inserting a fluid-filled pressure line through the tube. Esophageal pressure is easily recorded with a fluid-filled Salem double-lumen catheter. Large changes in lung compliance may pass unnoticed when only total compliance is monitored. Monitoring of lung compliance may offer an improved basis for decisions about ventilator settings.
|
['Adult', 'Aged', 'Airway Resistance', 'Anesthesia', 'Compliance', 'Esophagus', 'Female', 'Humans', 'Intubation', 'Lung Compliance', 'Male', 'Middle Aged', 'Positive-Pressure Respiration', 'Pressure', 'Respiration, Artificial', 'Respiratory Mechanics', 'Thorax', 'Tidal Volume', 'Trachea']
| 10,081,537
|
[['M01.060.116'], ['M01.060.116.100'], ['E01.370.386.700.050', 'G09.772.060'], ['E03.155'], ['G01.374.590.210'], ['A03.556.875.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.585', 'E05.497'], ['E01.370.386.700.475', 'G09.772.540'], ['M01.060.116.630'], ['E02.041.625.790', 'E02.880.820.790'], ['G01.374.715'], ['E02.041.625', 'E02.365.647.729', 'E02.880.820'], ['G09.772.705.700'], ['A01.923.761'], ['E01.370.386.700.485.750.900.350.750', 'G09.772.850.970.500.700'], ['A04.889']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Organisms [B]']
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Intraoral vertical subcondylar osteotomy: a national survey.
|
The results of a national survey pertaining to the IVSO are presented and the findings are contrasted with the literature on the procedure. It was found that the IVSO is a popular procedure for mandibular setback, that the value of determining the mandibular divergence angle is equivocal, that the Stryker oscillating saw is the most popular instrument for performing the osteotomy, that most operators leave the segments unfixed, and that the intraoral approach to the correction of mandibular prognathism is preferred. There were no geographic trends noted.
|
['Humans', 'Immobilization', 'Mandible', 'Mandibular Condyle', 'Osteotomy', 'Prognathism', 'Surgery, Oral', 'United States']
| 3,858,482
|
[['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.472'], ['A02.835.232.781.324.502.632', 'A14.521.632'], ['A02.835.232.781.324.502.632.600', 'A14.521.632.600'], ['E04.555.580'], ['C05.500.460.655', 'C05.500.607.655', 'C05.660.207.540.460.655', 'C07.320.440.655', 'C07.320.610.655', 'C07.650.500.460.655', 'C16.131.621.207.540.460.655', 'C16.131.850.500.460.655'], ['E06.892', 'H02.163.876.886'], ['Z01.107.567.875']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Diseases [C]', 'Disciplines and Occupations [H]', 'Geographicals [Z]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
|
[Part of the matrix on the 5' side from codon in the E-segment is close to protein S26 on the human 80S ribosome].
|
Positioning of mRNA on the 80S ribosome upstream the E site bound codon was studied using derivatives of nona- and dodecaribonucleotides containing the triplet UUU coding for Phe at the 3'-terminus, and a perfluorophenylazide cross-linker on either the first or the third nucleotide. Two sets of the mRNA analogues were used, with the photoactivatable groups on either the C5 atom of the uridine or the N7 atom of the guanosine. The modified nucleotides were directed to positions from -4 to -9 with respect to the first nucleotide of the P site bound codon by tRNA(Phe) cognate to the triplet UUU targeted to the P site. Mild UV-irradiation of ribosomecomplexes with tRNA(Phe) and mRNA analogues resulted in the cross-linking to the 40S subunits preferentially, mainly to the proteins. The principal target for the cross-linking was protein S26 in all cases. Location of the photoactivatable group on the nucleotide at position -4 lead also to the minor cross-linking to protein S3, and at position -6 to protein S14. In the absence of tRNA, all mRNA analogues cross-linked to protein S3.
|
['Codon', 'Electrophoresis, Gel, Two-Dimensional', 'Humans', 'RNA, Messenger', 'Ribosomal Proteins', 'Ribosomes']
| 15,612,591
|
[['D13.444.735.544.355', 'G05.360.335.355', 'G05.360.340.024.340.137.190'], ['E05.196.401.250', 'E05.301.300.230'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D13.444.735.544'], ['D12.776.835'], ['A11.284.430.214.190.875.811']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[The Freiburg Scarlet Fever Scheme, a guide for clinical practice in frequent occurrence of Streptococcus-A infections in schools and other community facilities according to paragraphs 44-48 of the Federal Infection Legislation].
|
In the past years, the endemic occurrence of A-streptococcal infections in communal institutions for children and other reasons called for an increased number of activities to combat such diseases. In view of the multitude of different epidemiological situations, differentiated suggestions favouring a holistic strategy to combat such infections were elaborated, e.g. with regard to therapy, prophylaxis and the practice of readmission to community institutions. The methods of application have still to be improved upon.
|
['Adolescent', 'Child', 'Communicable Disease Control', 'Germany', 'Humans', 'Public Health', 'Scarlet Fever', 'Schools']
| 2,146,558
|
[['M01.060.057'], ['M01.060.406'], ['N06.850.780.200'], ['Z01.542.315'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['H02.403.720', 'N01.400.550', 'N06.850'], ['C01.150.252.410.890.823'], ['I02.783', 'J03.832']]
|
['Named Groups [M]', 'Health Care [N]', 'Geographicals [Z]', 'Organisms [B]', 'Disciplines and Occupations [H]', 'Diseases [C]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Technology, Industry, and Agriculture [J]']
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
| 0
| 1
| 1
| 1
|
[Forms of histograms constructed from measurements of alpha-decay of 228Ra in Lindau (Germany) and neutron fluxes in Moscow change synchronously according to the local time].
|
In joint experiments performed at Max Plank Institute of Aeronomy (Germany) and the Institute of Theoretical and Experimental Biophysics in Pushchino, the main manifestations of the phenomenon of macroscopic fluctuations were confirmed. An increased probability of the similarity in synchronous histograms in independent measurements performed by two installations in one laboratory and in two laboratories separated by a distance of 2000 km was shown. In the latter case, the similarity of histograms is most probable at the same local time.
|
['Alpha Particles', 'Neutrons', 'Radium', 'Statistical Distributions', 'Time']
| 11,605,368
|
[['G01.750.750.055'], ['G01.249.660.250'], ['D01.268.271.770', 'D01.268.552.775', 'D01.268.556.775', 'D01.496.749.305.770', 'D01.552.539.745', 'D01.552.544.775'], ['E05.318.740.994', 'G17.820', 'N05.715.360.750.750', 'N06.850.520.830.994'], ['G01.910']]
|
['Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Replication of feline coronaviruses in peripheral blood monocytes.
|
Feline infectious peritonitis virus (FIPV) (Coronaviridae) causes the most lethal viral infection in cats: FIP. The related feline enteric coronavirus (FECV) causes mild enteritis. Why these feline coronaviruses manifest so differently in vivo is not known. In this study, infection kinetics (titres and antigen expression) of FIPV 79-1146, and FECV 79-1683, were determined in peripheral blood monocytes from 3 donor cats and compared to those in Crandell feline kidney (CrFK) cells. The infection kinetics in monocytes were host dependent. Monocytes from 1 cat were resistant to both FIPV- and FECV-infection. Monocytes from the other 2 cats could initially be infected by both FIPV and FECV but FIPV infection was sustained in monocytes of only one cat. FECV-infection was never sustained and viral production was up to 100 times lower than in FIPV-infected monocytes. In CrFK cells, FIPV and FECV infection kinetics did not differ. In monocytes of a larger cat population (n = 19) the 3 infection patterns were also found. Considering all 22 investigated cats, 3/22 were not susceptible for FIPV and FECV. The rest could be infected with FECV and FIPV but 10/22 cats had monocytes that only sustained FIPV infection and 9/22 sustained neither FIPV nor FECV infection.
|
['Animals', 'Cat Diseases', 'Cats', 'Cells, Cultured', 'Coronavirus Infections', 'Coronavirus, Feline', 'Feline Infectious Peritonitis', 'Female', 'Male', 'Microscopy, Confocal', 'Monocytes', 'Viral Proteins', 'Virus Replication']
| 16,052,283
|
[['B01.050'], ['C22.180'], ['B01.050.150.900.649.313.750.377.750.250.125'], ['A11.251'], ['C01.925.782.600.550.200'], ['B04.820.578.500.540.150.075.500.375'], ['C01.925.782.600.550.200.360', 'C22.180.440'], ['E01.370.350.515.395', 'E05.595.395'], ['A11.118.637.555.652', 'A11.148.580', 'A11.627.624', 'A11.733.547', 'A15.145.229.637.555.652', 'A15.378.316.580', 'A15.382.490.555.652', 'A15.382.670.547', 'A15.382.680.547'], ['D12.776.964'], ['G06.920.925']]
|
['Organisms [B]', 'Diseases [C]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Congenital hemangiopericytoma.
|
The clinicopathologic features of a case of congenital hemangiopericytoma are described and compared with those of previously reported cases. Features connotative of malignancy in adult or childhood hemangiopericytomas, such as high mitotic activity, increased cellularity, and tissue necrosis or hemorrhage, are frequently observed in congenital tumors. However, the congenital tumors are biologically benign. Spontaneous tumor regression observed in our case further suggests a conservative approach in the treatment of congenital hemangiopericytomas.
|
['Hemangiopericytoma', 'Humans', 'Infant, Newborn', 'Neoplasm Regression, Spontaneous', 'Soft Tissue Neoplasms']
| 3,713,189
|
[['C04.557.645.380'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703.520'], ['C04.697.670', 'C23.550.727.670', 'G16.767.500'], ['C04.588.839']]
|
['Diseases [C]', 'Organisms [B]', 'Named Groups [M]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
The role of the spleen in the rejection and enhancement of renal allografts in the rat.
|
As rats were splenectomised up to 7 weeks before or at the time of receiving an (AS times August)F1 kidney allograft. Only 2 of 19 splenectomised recipients rejected their grafts within 12 days, and 14 of the 19 survived for more than 100 days. All 19 splenectomised recipients had a marked and often complete suppression of the lymphocytotoxic antibody response to the graft. In contrast, splenecotomy had no effect in suppressing the rejection of (AS times August)F1 renal allografts transplanted to AS recipients previously immunised with August lymphoid tissue, or in AS recipients of homozygous August renal allografts. Splenectomised recipients could be reconstituted with spleen cells from sensitised but not from normal AS rats. Splenectomy was found neither to augment nor diminish the effectiveness of passive enhancement of (AS times August)F1 or August kidneys in AS recipients. Splenectomy of AS recipients 2 or 4 days after an (AS times August)F1 renal allograft was as effective as pregraft splenectomy in suppressing rejection, but there was a deficiency of long survivors in a group splenectomised 1 day after grafting. A working hypothesis to explain these results is presented, and their clinical relevance is discussed.
|
['Animals', 'Antibody Formation', 'Chimera', 'Homozygote', 'Kidney Transplantation', 'Male', 'Rats', 'Spleen', 'Splenectomy', 'Time Factors', 'Transplantation Immunology', 'Transplantation, Homologous']
| 1,099,728
|
[['B01.050'], ['G12.450.050.370.250'], ['B05.200'], ['G05.380.554'], ['E02.870.500', 'E04.936.450.485', 'E04.950.774.400'], ['B01.050.150.900.649.313.992.635.505.700'], ['A10.549.700', 'A15.382.520.604.700'], ['E04.726'], ['G01.910.857'], ['G12.875'], ['E04.936.864']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]']
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
An investigation of the evolutionary origin of reciprocal communication using simulated autonomous agents.
|
How does communication originates in a population of originally non-communicating individuals? Providing an answer to this question from a neo-Darwinian epistemological perspective is not a trivial task. The reason is that, for non-communicating agents, the capabilities of emitting signals and responding to them are both adaptively neutral traits if they are not simultaneously present. Research studies based on rather general and theoretically oriented evolutionary simulation models have, so far, demonstrated that at least two different processes can account for the origin of communication. On the one hand, communicative behaviour may first evolve in a non-communicative context and only subsequently acquire its adaptive function.On the other hand, communication may originate thanks to cognitive constraints; that is, communication may originate thanks to the existence of neural substrates that are common to the signalling and categorising capabilities. This article provides a proof-of-concept demonstration of the origin of communication in a novel-simulated scenario in which groups of two homogeneous (i.e. genetically identical) agents exploit reciprocal communication to develop common perceptual categories nd to perform a collective task. In particular, in circumstances in which communication is evolutionarily advantageous, simulated agents evolve from scratch social behaviour through acoustic interactions.We look into the phylogeny of successful communication protocol, and we describe the evolutionary phenomena that, in early evolutionary stages, paved the way for the subsequent development of reciprocal communication, categorisation capabilities and successful cooperative strategies.
|
['Algorithms', 'Animals', 'Auditory Perception', 'Behavior, Animal', 'Biological Evolution', 'Communication', 'Computer Simulation', 'Humans', 'Mathematical Concepts', 'Models, Theoretical', 'Robotics', 'Social Behavior', 'Vocalization, Animal']
| 19,730,879
|
[['G17.035', 'L01.224.050'], ['B01.050'], ['F02.463.593.071', 'G07.888.125'], ['F01.145.113'], ['G05.045', 'G16.075'], ['F01.145.209', 'L01.143'], ['L01.224.160'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G17'], ['E05.599'], ['H01.671.293.643', 'J01.897.104.834', 'L01.224.050.375.630'], ['F01.145.813'], ['F01.145.113.055.800']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Organisms [B]', 'Psychiatry and Psychology [F]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Technology, Industry, and Agriculture [J]']
| 0
| 1
| 0
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
|
Alveolar macrophages fat stain in early diagnosis of fat embolism syndrome.
|
The aim of this study was to assess the contribution of bronchoalveolar lavage (BAL) in the diagnosis of fat embolism syndrome (FES). The presence of fat droplets in alveolar macrophages was addressed in 13 trauma patients with bone fractures and 10 non-trauma patients with acute respiratory distress syndrome (ARDS). The control group was composed of 5 anesthesized patients with ischemic heart disease, immediately prior to cardiac surgery. Two patients with suggestive clinical and laboratory signs of FES had 40% and 24% fat-containing alveolar cells, respectively. The trauma patients without signs of FES displayed a wide variation in the percentage of fat-containing macrophages (from 3% to 95%). Most of the patients with ARDS who were receiving lipid emulsion as part of their parenteral nutrition, had a high percentage (> 85%) of fat-containing macrophages. Patients with normal lungs had no fat-containing macrophages. Our findings suggest that BAL Oil Red O-positive macrophages are frequently observed in trauma patients irrespective of the presence of FES. Therefore, estimation of the percentage of fat-containing macrophages from BAL is an unreliable marker of FES.
|
['Adult', 'Aged', 'Biomarkers', 'Bronchoalveolar Lavage Fluid', 'Case-Control Studies', 'Embolism, Fat', 'Female', 'Fractures, Bone', 'Humans', 'Lipids', 'Macrophages, Alveolar', 'Male', 'Middle Aged', 'Prospective Studies', 'Reproducibility of Results', 'Respiratory Distress Syndrome', 'Severity of Illness Index', 'Syndrome', 'Trauma Severity Indices']
| 9,397,138
|
[['M01.060.116'], ['M01.060.116.100'], ['D23.101'], ['E05.927.100.500'], ['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['C14.907.355.350.454'], ['C26.404'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D10'], ['A11.329.372.600', 'A11.627.482.600', 'A11.733.397.600', 'A15.382.670.522.600', 'A15.382.680.397.600'], ['M01.060.116.630'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['E05.318.370.725', 'E05.337.851', 'N05.715.360.325.685', 'N06.850.520.445.725'], ['C08.381.840', 'C08.618.840'], ['E05.318.308.980.438.475.456.500', 'N05.715.360.300.800.438.375.364.500', 'N06.850.520.308.980.438.475.364.500'], ['C23.550.288.500'], ['E05.318.308.940.968.875', 'E05.944', 'N04.452.859.564.800', 'N05.715.360.300.715.500.800', 'N06.850.520.308.940.968.875']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Diseases [C]', 'Organisms [B]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Newborn infant parasympathetic evaluation (NIPE) as a predictor of hemodynamic response in children younger than 2 years under general anesthesia: an observational pilot study.
|
BACKGROUND: It is still unknown whether newborn infant parasympathetic evaluation (NIPE), based on heart rate variability (HRV) as a reflection of parasympathetic nerve tone, can predict the hemodynamic response to a nociception stimulus in children less than 2 years old.METHODS: Fifty-five children undergoing elective surgery were analyzed in this prospective observational study. Noninvasive mean blood pressure (MBP), heart rate (HR) and NIPE values were recorded just before and 1 min after general anesthesia with endotracheal intubation as well as skin incision. The predictive performance of NIPE was evaluated by receiver-operating characteristic (ROC) curve analysis. A significant hemodynamic response was defined by a > 20% increase in HR and/or MBP.RESULTS: Endotracheal intubation and skin incision caused HR increases of 22.2% (95% confidence interval [CI] 17.5-26.9%) and 3.8% (2.1-5.5%), MBP increases of 18.2% (12.0-24.4%) and 10.6% (7.7-13.4%), and conversely, NIPE decreases of 9.9% (5.3-14.4%) and 5.6% (2.1-9.1%), respectively (all P < 0.01 vs. pre-event value). Positive hemodynamic responses were observed in 32 patients (62.7%) during tracheal intubation and 13 patients (23.6%) during skin incision. The area under the ROC curve values for the ability of NIPE to predict positive hemodynamic responses at endotracheal intubation and skin incision were 0.65 (0.50-0.78) and 0.58 (0.44-0.71), respectively.CONCLUSIONS: NIPE reflected nociceptive events as well as anesthestic induction in children less than 2 years undergoing general anaesthetia. Nevertheless, NIPE may not serve as a sensitive and specific predictor to changes in hemodynamics.TRIAL REGISTRATION: This study was registered on May 3, 2018 in the Chinese Clinical Trail Registry; the registration number is ( ChiCTR1800015973 ).
|
['Anesthesia, General', 'Blood Pressure', 'Female', 'Heart Rate', 'Hemodynamics', 'Humans', 'Infant', 'Male', 'Parasympathetic Fibers, Postganglionic', 'Pilot Projects', 'Predictive Value of Tests', 'Prospective Studies']
| 31,185,928
|
[['E03.155.197'], ['E01.370.600.875.249', 'G09.330.380.076'], ['E01.370.600.875.500', 'G09.330.380.500'], ['G09.330.380'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703'], ['A08.675.127.500.700', 'A08.675.542.100.700', 'A08.675.542.234.700', 'A08.800.050.050.050.700', 'A08.800.050.600.650', 'A08.800.800.060.050.700', 'A11.671.188.500.700', 'A11.671.501.100.700', 'A11.671.501.234.700'], ['E05.318.372.750', 'E05.337.737', 'N05.715.360.330.720', 'N06.850.520.450.720'], ['E05.318.370.800.650', 'N05.715.360.325.700.640', 'N06.850.520.445.800.650'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Named Groups [M]', 'Anatomy [A]', 'Health Care [N]']
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Low doses of gamma radiation in the management of postharvest Lasiodiplodia theobromae in mangos.
|
The postharvest life of mango is limited by the development of pathogens, especially fungi that cause rot, among which stands out the Lasiodiplodia theobromae. Several control methods have been employed to minimize the damages caused by this fungus, chemical control can leave residues to man and nature; physical control by the use of gamma radiation in combination with modified atmosphere and cold storage. The use of gamma radiation helps to reduce the severity of the pathogen assist in the ripening process of fruits, even at low doses (0.25, 0.35 and 0.45 kGy) chemical properties such as pH, soluble solids, acid ascorbic, titratable acidity and also the quality parameters of the pulp showed no damage that are ideal for trade and consumption of mangoes. This treatment can be extended for use in the management of diseases such as natural infections for penducular rot complex that has as one of L. theobroma pathogens involved.
|
['Ascomycota', 'Disinfection', 'Food Handling', 'Food Microbiology', 'Gamma Rays', 'Mangifera', 'Plant Diseases']
| 26,413,068
|
[['B01.300.107'], ['N06.850.780.200.450.850.375'], ['J01.576.423.200'], ['H01.158.273.540.274.332', 'J01.576.423.850.730.500.249.300', 'N06.850.425.200', 'N06.850.460.400.300', 'N06.850.601.500.249.300'], ['G01.358.500.505.300', 'G01.750.250.300', 'G01.750.750.400'], ['B01.650.940.800.575.912.250.044.416'], ['G15.610']]
|
['Organisms [B]', 'Health Care [N]', 'Technology, Industry, and Agriculture [J]', 'Disciplines and Occupations [H]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
|
Single Restorations in the Resorbed Posterior Mandible Supported by 6-mm Implants: A 1-Year Prospective Case Series Study.
|
PURPOSE: The aim of this prospective case series study was to assess the clinical performance of single restorations in the posterior mandible supported by 6-mm implants.MATERIALS AND METHODS: Twenty-one consecutive patients with one or more missing premolars or molars in the posterior mandible and with an estimated bone height of 8 mm between the top of the ridge and alveolar nerve were included. Each patient was to receive one or more 6-mm implants (OsseoSpeed 4.0 S, Astra Tech Implant System, Dentsply Implants, M?lndal, Sweden) without any grafting. After a 3-month osseointegration period, the implants were restored with custom-made titanium abutments (Atlantis abutment, Dentsply Implants) and cemented zirconia-based porcelain crowns. Clinical and radiographic examinations were performed at placement of the restoration and 12 months thereafter. Next to this, patients' satisfaction was scored before treatment and after 12 months with the restoration in function.RESULTS: Implant survival was 100%. From loading to the 12-month follow-up, mean marginal bone loss was 0.14 mm (SD: 0.4). Mean scores for plaque, calculus, gingiva, bleeding, and pocket probing depth were low. Patients' satisfaction was high.CONCLUSION: One-year follow-up data revealed that 6-mm dental implants inserted in the resorbed posterior mandible provide a solid basis for single tooth restoration.
|
['Adult', 'Aged', 'Dental Implantation, Endosseous', 'Dental Implants, Single-Tooth', 'Dental Prosthesis, Implant-Supported', 'Dental Restoration, Permanent', 'Female', 'Humans', 'Male', 'Mandible', 'Middle Aged', 'Prospective Studies']
| 25,195,816
|
[['M01.060.116'], ['M01.060.116.100'], ['E04.545.550.280.280', 'E04.650.230.500', 'E06.645.550.280.280', 'E06.780.314.310'], ['E06.780.346.593.185', 'E07.695.185.185'], ['E06.780.346.706', 'E07.695.190.185'], ['E06.323.428', 'E06.780.346.737', 'E07.695.190.190'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A02.835.232.781.324.502.632', 'A14.521.632'], ['M01.060.116.630'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Anatomy [A]', 'Health Care [N]']
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Distinct breakfast patterns on satiety perception in individuals with weight excess.
|
OBJECTIVES: Western dietary pattern predisposes to weight gain, insulin resistance and cardiometabolic diseases. Promoting satiety via modifications in diet composition could be useful to fight weight gain. Mediterranean diet which is recognized to be cardioprotective contains high fiber and unsaturated fat contents. We compared the effects of distinct breakfast patterns on satiety of individuals at cardiometabolic risk, and examined the correlation of satiety level after each breakfast intervention period with glucose parameters.MATERIALS AND METHODS: In this 10-week cross-over clinical trial, 54 individuals with weight excess were submitted to 2 types of 4-week isocaloric breakfasts (2-week washout), one typically Brazilian and a modified one, differing concerning fiber and types of fatty acids contents. Clinical data were collected before and after each breakfast. A satiety scale was applied at fasting and 10, 30 and 120' after breakfast consumption. Repeated measures ANOVA, Student t test or non-parametric correspondents were used; correlations were tested by Pearson or Spearman coefficients.RESULTS: Anthropometric variations after breakfasts were not significant. Only after the modified breakfast, reduction in blood pressure levels was observed. The satiety level did not show significant variation across each period or between the breakfasts. Non-significant correlation between satiety and glucose, insulin and HOMA-IR values after each intervention period was observed.CONCLUSION: We conclude that different breakfast compositions do not alter satiety level, which is not correlated to glucose parameters in overweight individuals. Stronger modifications of daily meals might be necessary to differentiate satiety levels under distinct dietary patterns.
|
['Adult', 'Aged', 'Analysis of Variance', 'Anthropometry', 'Blood Glucose', 'Blood Pressure', 'Brazil', 'Breakfast', 'Cholesterol', 'Cross-Over Studies', 'Diet, Mediterranean', 'Diet, Western', 'Energy Intake', 'Female', 'Humans', 'Insulin', 'Insulin Resistance', 'Male', 'Middle Aged', 'Overweight', 'Reference Values', 'Satiation', 'Statistics, Nonparametric', 'Time Factors', 'Triglycerides']
| 26,910,624
|
[['M01.060.116'], ['M01.060.116.100'], ['E05.318.740.150', 'N05.715.360.750.125', 'N06.850.520.830.150'], ['E01.370.600.024', 'E05.041', 'N06.850.505.200.100'], ['D09.947.875.359.448.500'], ['E01.370.600.875.249', 'G09.330.380.076'], ['Z01.107.757.176'], ['G07.203.300.590.120', 'J02.500.590.120'], ['D04.210.500.247.222.284', 'D04.210.500.247.808.197', 'D10.570.938.208'], ['E05.318.370.150', 'N05.715.360.325.150', 'N06.850.520.445.150'], ['E02.642.249.270', 'G07.203.650.240.270'], ['G07.203.650.240.310'], ['G07.203.650.240.340'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D06.472.699.587.200.500.625', 'D12.644.548.586.200.500.625'], ['C18.452.394.968.500', 'G07.690.773.984.617'], ['M01.060.116.630'], ['C23.888.144.699', 'E01.370.600.115.100.160.120.699', 'G07.100.100.160.120.699'], ['E05.978.810'], ['F02.830.749'], ['E05.318.740.995', 'N05.715.360.750.760', 'N06.850.520.830.995'], ['G01.910.857'], ['D10.351.801']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Geographicals [Z]', 'Technology, Industry, and Agriculture [J]', 'Organisms [B]', 'Diseases [C]', 'Psychiatry and Psychology [F]']
| 0
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 1
| 1
|
Pig-a mutation: kinetics in rat erythrocytes following exposure to five prototypical mutagens.
|
An in vivo mutation assay has been developed based on flow cytometric enumeration of glycosylphosphatidylinositol (GPI) anchor-deficient rat erythrocytes. With this method, blood is incubated with anti-CD59-PE and SYTO 13 dye, and flow cytometry is used to score the frequency of CD59-negative erythrocytes. The experiments described herein were designed to define the kinetics of mutant erythrocyte appearance and disappearance from peripheral blood to support appropriate treatment and sampling designs for the assay. Wistar Han rats were treated with one of five prototypical mutagens: N-ethyl-N-nitrosourea (ENU); 7,12-dimethyl-1,2-benz[a]anthracene (DMBA); 4-nitroquinoline-1-oxide; benzo[a]pyrene; and N-methyl-N-nitrosourea. ENU and DMBA were also evaluated in Sprague Dawley rats. Animals were treated on three consecutive days (days 1-3) via oral gavage, and blood specimens were obtained on days -1, 4, 15, 30, 45, and 90 (and day 180 for ENU). A second endpoint of genotoxicity, the frequency of peripheral blood micronucleated reticulocytes, was measured on day 4. Each chemical induced micronuclei and the GPI anchor-deficient phenotype. Increased mutant cell frequencies were evident at day 15. Mutant reticulocyte frequencies remained relatively stable for some chemicals, but others peaked and then dropped significantly. The differences in kinetics observed are presumably related to the degree to which mutation occurs in hematopoietic stem cells versus more committed cells with limited self-renewal capacity. Collectively, the results suggest that enumerating GPI anchor-deficient erythrocytes is an efficient means of evaluating the in vivo mutagenic potential of chemicals. The kinetics and ease of scoring this blood-based endpoint suggest that integration into routine toxicology studies will be feasible.
|
['4-Nitroquinoline-1-oxide', '9,10-Dimethyl-1,2-benzanthracene', 'Animals', 'Benzo(a)pyrene', 'Erythrocytes', 'Ethylnitrosourea', 'Membrane Proteins', 'Methylnitrosourea', 'Mutagenicity Tests', 'Mutagens', 'Mutation', 'Rats', 'Rats, Sprague-Dawley', 'Rats, Wistar']
| 19,965,957
|
[['D02.640.820.600', 'D03.633.100.810.470.450', 'D03.661.243.500'], ['D02.455.426.559.847.149.301', 'D04.615.149.301'], ['B01.050'], ['D02.455.426.559.847.799.306.300', 'D04.615.799.306.300'], ['A11.118.290', 'A11.443.240', 'A15.145.229.334'], ['D02.065.950.594.310', 'D02.654.692.300'], ['D12.776.543'], ['D02.065.950.594.490', 'D02.654.692.480'], ['E05.393.560', 'E05.940.560'], ['D27.888.569.468'], ['G05.365.590'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.750'], ['B01.050.150.900.649.313.992.635.505.700.900']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
The feasibility of putrescible components of municipal solid waste for biomethane production at Hyderabad, Pakistan.
|
This study analyzes the feasibility of putrescible components of municipal solid waste (PCMSW) such as food waste (FW) and yard waste (YW) for methane production in Pakistan. The batch experiments have been conducted at two different inoculums to substrate ratios (ISRs) by using various inoculums under mesophilic condition. The highest methane yield of FW and YW is achieved to be 428 Nml g-1 volatile solids (VS) added and 304 Nml g-1 VS added respectively by using buffalo dung inoculum at ISR-5. While, lowest methane yield of FW and YW is obtained as 236 Nml g-1 VS added and 151Nml g-1 VS added respectively by using effluent from a continuous stirrer tank reactor as inoculum at ISR-3. The first order decay model has been introduced, which gives best fit for methane potential of PCMSW with buffalo dung inoculum. Additionally, the feasibility of PCMSW in terms of power generation potential has been analyzed. About 60.63 million m3/year energy can be generated by converting PCMSW into methane gas leading to power generation. The finding of this study concludes that the replacement of imported energy and reduction up to 1.62% in other primary energy sources would be achieved, if PCMSW are properly converted into energy through anaerobic digestion in Pakistan.
|
['Anaerobiosis', 'Bioreactors', 'Methane', 'Pakistan', 'Solid Waste']
| 29,313,447
|
[['G02.111.062', 'G03.078'], ['E07.115', 'J01.897.120.115'], ['D02.455.326.146.571'], ['Z01.252.245.723'], ['D20.944.730', 'N06.850.460.710.730']]
|
['Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Technology, Industry, and Agriculture [J]', 'Chemicals and Drugs [D]', 'Geographicals [Z]', 'Health Care [N]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
|
Characterization of antisera against bovine prolactin for in vivo studies on prolactin function in the rat.
|
The IgG fraction of rabbit antisera to bovine prolactin (PRL), intended for in vivo studies on the role of PRL in the rat, was prepared and characterized in vitro and in vivo. The antibodies showed a strong reaction with bovine PRL in double diffusion, immunoelectrophoresis, radioimmunoassay and passive haemagglutination using bovine PRL-coated erythrocytes. In indirect immunofluorescence on paraffin sections of bovine pituitary glands the antibodies could be used for the detection of PRL-producing cells. Cross-reaction with rat PRL was observed in passive haemagglutination with rat PRL-coated erythrocytes and in indirect immunofluorescence on rat pituitary gland, but not in any of the other test systems. The ability of the antibodies to neutralize homologous, i.e. bovine, PRL was tested in lactating rats depleted of endogenous PRL by bromergocriptin treatment. The impaired lactation performance of such animals can be restored by substitution with bovine PRL. If the bovine PRL used for substitution was complexed with anti-bovine PRL-IgG, it lost its biological activity. On the other hand, injections of even high amounts of the antibodies into lactating rats failed to reveal any effect on lactation. It is concluded that either the antibodies do not cross-react with circulating rat PRL in contrast to pituitary PRL (preprolactin?) or that the cross-reacting antibody-populations(s) lack(s) the ability to neutralize the biological function of rat PRL.
|
['Animals', 'Antibodies', 'Cattle', 'Cross Reactions', 'Female', 'Fluorescent Antibody Technique', 'Immune Sera', 'Immunoglobulin G', 'Lactation', 'Pituitary Gland', 'Pregnancy', 'Prolactin', 'Rats']
| 7,002,391
|
[['B01.050'], ['D12.776.124.486.485.114', 'D12.776.124.790.651.114', 'D12.776.377.715.548.114'], ['B01.050.150.900.649.313.500.380.271'], ['G12.122.281'], ['E01.370.225.500.607.512.240', 'E01.370.225.750.551.512.240', 'E05.200.500.607.512.240', 'E05.200.750.551.512.240', 'E05.478.583.375'], ['A12.207.152.846.500', 'D12.776.124.486.485.114.573', 'D12.776.124.790.651.114.573', 'D12.776.377.715.548.114.573', 'D20.215.401'], ['D12.776.124.486.485.114.619.393', 'D12.776.124.790.651.114.619.393', 'D12.776.377.715.548.114.619.393'], ['G08.686.523', 'G08.686.702.500'], ['A06.300.747', 'A06.688.357.750', 'A08.186.211.180.497.352.435.500', 'A08.186.211.200.317.357.352.435.500', 'A08.713.357.750'], ['G08.686.784.769'], ['D06.472.699.322.576.773', 'D06.472.699.631.525.525', 'D12.644.548.691.525.525'], ['B01.050.150.900.649.313.992.635.505.700']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Maxillary sinus mucopyoceles.
|
Mucopyoceles of the maxillary sinus are seen rarely, and their origin has been the subject of much controversy. Three cases are reported herein, accompanied by a review of the literature, to clarify some aspects of their cause, diagnosis and treatment. It has been concluded that the most common cause of mucopyoceles is trauma, whether accidental or iatrogenic (ie, due to surgery performed on the affected sinus), and that the best surgical approach is a wide Caldwell-Luc sinusectomy.
|
['Adult', 'Aged', 'Humans', 'Male', 'Maxillary Sinus', 'Mucocele', 'Prognosis', 'Radiography']
| 6,704,033
|
[['M01.060.116'], ['M01.060.116.100'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A04.531.621.578'], ['C04.182.511'], ['E01.789'], ['E01.370.350.700']]
|
['Named Groups [M]', 'Organisms [B]', 'Anatomy [A]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
[Hodgkin's disease with epithelioid granulomatous reaction].
|
Hodgkin's disease with a conspicuous presence of epithelioid-histiocytic elements was described as a particular subvariant of mixed type Hodgkin's disease. The authors presented four new cases. An additional increase of Langerhan's and interdigitating cells was observed in two cases, sarcoidosis-like granulomas with epithelioid and big multinucleated cells in the third. This female died and granulomas were found in several other organs. A prognostic relevance of this conspicuous reaction has not been clear for the time being. The mentioned patient deceased one year after the diagnosis had been settled, other three patients have been clinically well surviving 2-5 years.
|
['Adult', 'Aged', 'Epithelioid Cells', 'Female', 'Histiocytes', 'Hodgkin Disease', 'Humans', 'Lymph Nodes', 'Male', 'Middle Aged']
| 9,750,420
|
[['M01.060.116'], ['M01.060.116.100'], ['A11.329.372.300', 'A11.627.482.300', 'A11.733.397.300', 'A15.382.670.522.300', 'A15.382.680.397.300'], ['A11.329.372.385', 'A11.627.482.385', 'A11.733.397.385', 'A15.382.670.522.385', 'A15.382.680.397.385'], ['C04.557.386.355', 'C15.604.515.569.355', 'C20.683.515.761.355'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A10.549.400', 'A15.382.520.604.412'], ['M01.060.116.630']]
|
['Named Groups [M]', 'Anatomy [A]', 'Diseases [C]', 'Organisms [B]']
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Structure of the mosquitocidal delta-endotoxin CytB from Bacillus thuringiensis sp. kyushuensis and implications for membrane pore formation.
|
The delta-endotoxin CytB, found in parasporal inclusions of Bacillus thuringiensis subspecies kyushuensis, is a membrane pore-forming protein which is lethal to the larvae of Dipteran insects and broadly cytolytic in vitro. The crystal structure of CytB in the protoxin form has been determined by isomorphous replacement using heavy-atom derivatives of both the wild-type protein and an engineered cysteine mutant. The atomic model comprising residues 19 to 245 and 28 bound water molecules has been refined at 2.6 angstrom resolution to a crystallographic R-factor of 19.7% and a free R-factor of 26.1%. CytB has a single domain of alpha/beta architecture but a novel connectivity comprising two outer layers of alpha-helix hairpins wrapped around a mixed beta-sheet. In the protoxin form, CytB is a dimer linked by the intertwined N-terminal strands in a continuous, 12-stranded beta-sheet. Proteolytic processing cleaves the intertwined beta-strands to release the active CytB as a monomer, as well as removing the C-terminal tail to uncover the three-layered core. The homologous toxin CytA should show the same fold. Mutations in CytA that inhibit expression map to the dimer contacts and to the tip of helix pair A-B in contact with the sheet, apparently preventing correct folding. Mutations that inhibit toxicity map to the edge of the beta-sheet adjoining the helix pair C-D and to the sheet face, while mutations on the helix surfaces have no effect. Therefore segments forming the sheet, rather than the amphiphilic but short helices, are responsible for membrane binding and pore formation. A conformational change is postulated by which the helix pair C-D peels away from the sheet to lie on the membrane surface, while the sheet region rearranges to form an oligomeric trans-membrane pore.
|
['Amino Acid Sequence', 'Bacillus thuringiensis', 'Bacillus thuringiensis Toxins', 'Bacterial Proteins', 'Bacterial Toxins', 'Crystallization', 'Endotoxins', 'Hemolysin Proteins', 'Models, Molecular', 'Molecular Sequence Data', 'Mutation', 'Porins', 'Protein Conformation']
| 8,632,451
|
[['G02.111.570.060', 'L01.453.245.667.060'], ['B03.300.390.400.158.218.800', 'B03.353.500.100.218.800', 'B03.510.100.100.218.800', 'B03.510.415.400.158.218.800', 'B03.510.460.410.158.218.800'], ['D23.946.123.090'], ['D12.776.097'], ['D23.946.123'], ['E05.196.300', 'G02.171'], ['D23.946.123.329'], ['D12.776.543.695.444'], ['E05.599.595'], ['L01.453.245.667'], ['G05.365.590'], ['D12.776.157.530.400.500', 'D12.776.543.550.450.730', 'D12.776.543.585.400.730'], ['G02.111.570.820.709']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
A non-canonical site reveals the cooperative mechanisms of microRNA-mediated silencing.
|
Although strong evidence supports the importance of their cooperative interactions, microRNA (miRNA)-binding sites are still largely investigated as functionally independent regulatory units. Here, a survey of alternative 3´UTR isoforms implicates a non-canonical seedless site in cooperative miRNA-mediated silencing. While required for target mRNA deadenylation and silencing, this site is not sufficient on its own to physically recruit miRISC. Instead, it relies on facilitating interactions with a nearby canonical seed-pairing site to recruit the Argonaute complexes. We further show that cooperation between miRNA target sites is necessary for silencing in vivo in the C. elegans embryo, and for the recruitment of the Ccr4-Not effector complex. Using a structural model of cooperating miRISCs, we identified allosteric determinants of cooperative miRNA-mediated silencing that are required for both embryonic and larval miRNA functions. Our results delineate multiple cooperative mechanisms in miRNA-mediated silencing and further support the consideration of target site cooperation as a fundamental characteristic of miRNA function.
|
["3' Untranslated Regions", 'Alternative Splicing', 'Animals', 'Argonaute Proteins', 'Base Sequence', 'Binding Sites', 'Caenorhabditis elegans', 'Embryo, Nonmammalian', 'Gene Silencing', 'MicroRNAs', 'Models, Molecular', 'Nucleic Acid Conformation', 'RNA-Induced Silencing Complex', 'Transcription Factors']
| 28,482,037
|
[['D13.444.735.544.875.880', 'D13.444.735.790.878.880', 'G05.360.340.024.220.880.880', 'G05.360.340.024.340.137.910.880'], ['G02.111.760.700.100', 'G03.839.700.100', 'G05.308.700.700.100'], ['B01.050'], ['D08.811.277.352.355.350.810.500', 'D08.811.277.352.700.350.810.500', 'D12.776.157.725.500.906.500', 'D12.776.664.962.500.906.500'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['G02.111.570.120'], ['B01.050.500.500.294.400.875.660.250.250'], ['A13.350', 'A16.331'], ['G05.308.203.374'], ['D13.150.650.319', 'D13.444.735.150.319', 'D13.444.735.790.552.500'], ['E05.599.595'], ['G02.111.570.820.486', 'G05.360.580'], ['D08.811.277.352.355.350.810', 'D08.811.277.352.700.350.810', 'D12.776.157.725.500.906', 'D12.776.664.962.500.906'], ['D12.776.930']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Information Science [L]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Antibodies to CBir1 are associated with glycogen storage disease type Ib.
|
OBJECTIVES: : Glycogen storage disease (GSD) type Ib is a congenital disorder of glycogen metabolism that is associated with neutropenia, neutrophil and monocyte dysfunction, and an inflammatory bowel disease (IBD) that mimics a Crohn disease phenotype. The enteric microflora is implicated in the pathogenesis of IBD; however, its role in the development of GSD-associated IBD is unknown. Antibody reactivity to Saccharomyces cerevisiae antibodies (ASCA), Escherichia coli outer membrane porin C (anti-OmpC), and bacterial flagellin (anti-CBir1) have been associated with Crohn disease in the general population, but they have an undetermined association in children and adults with GSD-Ib. Our goal was to examine the association of ASCA, anti-OmpC, and anti-CBir1 with the clinical features of GSD-Ib enterocolitis.PATIENTS AND METHODS: : A retrospective review identified 19 patients with GSD-Ib with or without a known diagnosis of enterocolitis. Radiographic, endoscopic, and serologic data were collected and assays for ASCA, anti-OmpC, and anti-CBir1 obtained.RESULTS: : Seven patients had combined radiographic, endoscopic, and histologic evidence of intestinal inflammation; the majority had ileocolonic involvement. Seventeen of 19 (89%) patients had elevated anti-CBir1 levels (6/7 in the IBD group and 11/12 in the no clinical evidence of IBD group). Thirteen of 19 (68%) had elevated anti-OmpC levels (5/7 in the IBD group and 8/12 in the no clinical evidence of IBD group). Eleven of 19 (58%) patients had elevated ASCA IgA levels (4/7 in the IBD group and 7/12 in the no clinical evidence of IBD group).CONCLUSIONS: : Nearly all of the patients with GSD-Ib had elevated anti-CBir1 levels. The antibody did not differentiate those with and without a diagnosis of GSD-Ib-associated IBD. Seroreactivity to flagellin may represent immune dysfunction rather than active enterocolitis in this patient population. Long-term follow-up of the group without known IBD is required to determine whether these antibodies can predict intestinal inflammation.
|
['Adolescent', 'Adult', 'Antibodies', 'Bacterial Proteins', 'Child', 'Child, Preschool', 'Enterocolitis', 'Female', 'Flagellin', 'Glycogen Storage Disease Type I', 'Humans', 'Incidence', 'Infant', 'Inflammatory Bowel Diseases', 'Intestines', 'Male', 'Retrospective Studies', 'Young Adult']
| 20,410,847
|
[['M01.060.057'], ['M01.060.116'], ['D12.776.124.486.485.114', 'D12.776.124.790.651.114', 'D12.776.377.715.548.114'], ['D12.776.097'], ['M01.060.406'], ['M01.060.406.448'], ['C06.405.205.596', 'C06.405.469.363'], ['D12.776.097.380'], ['C16.320.565.202.449.448', 'C18.452.648.202.449.448'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.308.985.525.375', 'N01.224.935.597.500', 'N06.850.505.400.975.525.375', 'N06.850.520.308.985.525.375'], ['M01.060.703'], ['C06.405.205.731', 'C06.405.469.432'], ['A03.556.124'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
[Essential tremor].
|
Essential tremor. Essential tremor is very common in the general population. It is a postural tremor occurring in adulthood, evolving progressively and whose severity can be very variable from one individual to another. A positive effect of alcohol is often reported. Its pathophysiology remains poorly understood but candidate genes have been identified in some families. From a therapeutic point of view, a first-line treatment based on propanolol or primidone may be prescribed in case of discomfort. In case of inefficiency or intolerance, second-line treatments are available but their anti-tremor effect is very variable, depending on patients. In case of pharmacoresistance, the stimulation of the VIM nucleus of the thalamus is a very effective neurosurgical option. In the case of a contraindication to stimulation, gammaknife thalamotomy (radiosurgery) may also be considered. Recently, ultrasound thalamotomy has been shown to be a promising therapy in essential tremor.
|
['Essential Tremor', 'Humans', 'Propranolol', 'Radiosurgery', 'Thalamus', 'Treatment Outcome', 'Ultrasonography']
| 30,869,259
|
[['C10.228.662.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D02.033.100.624.698.711', 'D02.033.755.624.698.711', 'D02.092.063.624.698.711', 'D02.455.426.559.847.638.945', 'D04.615.638.945'], ['E02.815.530', 'E04.525.800.650', 'E05.873.500'], ['A08.186.211.200.317.826'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['E01.370.350.850']]
|
['Diseases [C]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Health Care [N]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Biochemical and biological evaluation of novel potent coumarin inhibitor of 17â-HSD type 1.
|
Human 17â-hydroxysteroid dehydrogenase (17â-HSD) type 1 is an enzyme that acts at the pre-receptor level. It catalyzes the NADPH-dependent reduction of the weak estrogen estrone into the most potent estrogen 17â-estradiol, which exerts proliferative effects via estrogen receptors. Overexpression of 17â-HSD type 1 in estrogen-responsive tissues is related to the development of hormone-dependent diseases, such as breast cancer and endometriosis. 17â-HSD type 1 thus represents an attractive target for development of new drugs. Recently, we discovered that substituted coumarin derivatives potently and selectively inhibit 17â-HSD type 1. In the present study, we have performed additional biochemical and biological evaluation of the most promising coumarin derivative. First, we used an efficient method for isolation and purification of the active, soluble recombinant human 17â-HSD type 1 from Escherichia coli. This 17â-HSD type 1 showed a specific activity of 0.64±0.08 ìmol min(-1) mg(-1) for estrone reduction in the presence of NADPH at pH 6.5, and a K(m) of 62 nM for estrone. Next, we evaluated the best of the coumarin-derivative inhibitors, showing its reversible and competitive inhibition of 17â-HSD type 1 reductive activity with a K(i) of 53 nM. We confirmed that this coumarin inhibitor acts not only in a cell-free assay, but also decreases endogenous 17â-HSD type 1 activity in human T-47D breast cancer cells. This inhibitor also reduced estrone dependent growth of T-47D cells after 48 h of incubation.
|
['17-Hydroxysteroid Dehydrogenases', 'Cell Line, Tumor', 'Cell Proliferation', 'Coumarins', 'Drug Evaluation, Preclinical', 'Enzyme Inhibitors', 'Escherichia coli', 'Estrogens', 'Humans', 'Solubility']
| 21,232,530
|
[['D08.811.682.047.436.375'], ['A11.251.210.190', 'A11.251.860.180'], ['G04.161.750', 'G07.345.249.410.750'], ['D03.383.663.283.446', 'D03.633.100.150.446'], ['E05.290.750', 'E05.337.550'], ['D27.505.519.389'], ['B03.440.450.425.325.300', 'B03.660.250.150.180.100'], ['D27.505.696.399.472.277'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G02.805']]
|
['Chemicals and Drugs [D]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Nocardia nova aortitis after coronary artery bypass surgery.
|
Cardiac nocardiosis is a rare disease that is nearly always associated with cardiac operation. We report the case of a patient with a 10-month history of intermittent fevers after coronary artery bypass operation who presented with progressive shortness of breath and fever. He was found to have a large aortic aneurysm secondary to Nocardia nova infection likely transmitted during his original bypass operation. This is the first reported case of Nocardia aortitis after coronary bypass operation and serves to alert physicians of this rare but serious postoperative complication.
|
['Aged', 'Aortitis', 'Coronary Artery Bypass', 'Coronary Disease', 'Diagnosis, Differential', 'Echocardiography, Transesophageal', 'Humans', 'Male', 'Nocardia', 'Nocardia Infections', 'Surgical Wound Infection']
| 17,484,997
|
[['M01.060.116.100'], ['C14.907.109.320', 'C14.907.940.080'], ['E04.100.376.719.332', 'E04.100.814.868.750', 'E04.928.220.520.220'], ['C14.280.647.250', 'C14.907.585.250'], ['E01.171'], ['E01.370.350.130.750.235', 'E01.370.350.850.220.235', 'E01.370.370.380.220.235'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['B03.510.024.981.550'], ['C01.150.252.410.040.692'], ['C01.947.692', 'C23.550.767.925']]
|
['Named Groups [M]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
[The clinical picture of terminally ill patients with lung cancer].
|
An investigation has been made with regard to the clinical picture of 87 terminally ill patients with lung cancer. It has yielded the following points. 1) Seven patients had been informed of their diagnosis. 2) Intravenous hyperalimentation was administered in 78 cases (90%), oxygen therapy in 68 cases (78%), and morphine in 35 cases (40%). 3) The most frequent cause of death in these patients was respiratory failure, due to progress of cancer, then infection, pleural, or pericardial effusion, or interstitial pneumonitis. 4) Psychic disturbances involved anxiety over breathing, depression, and delirium. In only 12% of the patients did the mental condition seem normal until death. 5) To deal with the dying patient's needs, it is necessary to establish proper treatment for the control of sensory dyspnea and for psychosocial support by a psychiatrist and other professionals for members of the family.
|
['Adult', 'Aged', 'Aged, 80 and over', 'Anxiety', 'Cause of Death', 'Combined Modality Therapy', 'Delirium', 'Depression', 'Female', 'Humans', 'Lung Neoplasms', 'Male', 'Middle Aged', 'Morphine', 'Oxygen Inhalation Therapy', 'Parenteral Nutrition, Total', 'Respiratory Insufficiency', 'Social Support', 'Terminal Care']
| 2,501,534
|
[['M01.060.116'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['F01.470.132'], ['E05.318.308.985.550.250', 'N01.224.935.698.100', 'N06.850.505.400.975.550.250', 'N06.850.520.308.985.550.250'], ['E02.186'], ['C10.597.606.337.500', 'C23.888.592.604.339.500', 'F01.700.250.500', 'F03.615.350'], ['F01.145.126.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C04.588.894.797.520', 'C08.381.540', 'C08.785.520'], ['M01.060.116.630'], ['D03.132.577.249.562.571', 'D03.605.497.607.587', 'D03.633.400.686.607.587', 'D04.615.723.795.576.571'], ['E02.880.690'], ['E02.421.505.575', 'E02.642.500.505.750'], ['C08.618.846'], ['I01.880.853.500.600'], ['E02.760.905', 'N02.421.585.905']]
|
['Named Groups [M]', 'Psychiatry and Psychology [F]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Diseases [C]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Anthropology, Education, Sociology, and Social Phenomena [I]']
| 0
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 0
|
Septal myomectomy and coronary artery bypass in one case of hypertrophic obstructive cardiomyopathy.
|
UNLABELLED: Surgery of the hypertrophic cardiomyopathy remains controversial. The safest procedure and the simples procedure is the mitral valve replacement. Septal myomectomy is difficult because of the poor surgical exposure and complications: incomplete resection, complete heart block, ventricular septal defect.CASE PRESENTATION: A 56 years old man with hypertrophic cardiomyopathy, double coronary stenosis and grade II mitral regurgitation by systolic anterior motion was long time stable under treatment with b-blockers. He developed angina and the circumflex and the left anterior descending arteries were stented. Re-stenosis developed in the left anterior descending stent and the patient was referred to surgery. The intraventricular gradient was 80 mmHg and the maximal septal thickness 28 mm. He was successfully treated by septal myomectomy and bypass on the left anterior descending artery with the left internal thoracic artery. Perioperative transesophageal echography was used to establish the limits of the surgical resection and to control the remnant gradient. The patient is asymptomatic 6 months after the procedure, he has a 30 mmHg remnant gradient and a grade I mitral regurgitation.CONCLUSION: Septal myomectomy is a safe alternative to mitral valve replacement for hypertrophic cardiomyopathy. This procedure must be guided by perioperative transesophageal echography to avoid incomplete resection.
|
['Cardiomyopathy, Hypertrophic', 'Coronary Artery Bypass', 'Echocardiography, Transesophageal', 'Humans', 'Male', 'Mammary Arteries', 'Middle Aged', 'Mitral Valve Insufficiency', 'Perioperative Care', 'Treatment Outcome', 'Ventricular Septum']
| 20,201,256
|
[['C14.280.238.100', 'C14.280.484.048.750.070.160'], ['E04.100.376.719.332', 'E04.100.814.868.750', 'E04.928.220.520.220'], ['E01.370.350.130.750.235', 'E01.370.350.850.220.235', 'E01.370.370.380.220.235'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A07.015.114.891.525'], ['M01.060.116.630'], ['C14.280.484.461'], ['E02.760.731', 'E04.604', 'N02.421.585.722'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['A07.541.459.750']]
|
['Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Anatomy [A]', 'Named Groups [M]', 'Health Care [N]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Delayed blood pressure recovery after head-up tilting during sleep in preterm infants.
|
Dramatic changes in cardiovascular control occur in sleep during infancy, when sleep time is at a lifetime maximum. In infants born preterm there are significant cardiovascular complications later in life, and also an increased risk for sudden infant death syndrome (SIDS), possibly a result of inadequate compensation to a cardiorespiratory challenge in sleep. We aimed to examine the consequences of preterm birth on heart rate (HR) and blood pressure (BP) responses to head-up tilting (HUT) during sleep in infants. Preterm (n = 25) and term (n = 20) infants were studied using daytime polysomnography at 2-4 weeks', 2-3 months' and 5-6 months' term-corrected age (CA). BP was recorded using a photoplethysmographic cuff (Finometer); 15 degrees HUTs were performed during both quiet and active sleep. Preterm infants responded to HUT with increased HR and BP, followed by a bradycardia and a subsequent return of HR and BP to baseline. Overall, HUT responses were similar between term and preterm infants at matched ages. Notably, however, return of BP to baseline was considerably delayed in preterm infants ( approximately 37 beats post-tilt) compared with term infants ( approximately 23 beats post-tilt) at both 2-4 weeks' and 2-3 months' CA (P < 0.05). Our study has demonstrated that preterm infants respond to a BP perturbation with changes in HR that match those of term infants. However, delayed recovery of BP during sleep in the preterm infant may be indicative of underlying deficits or immaturity in vascular function or control, which may contribute to their vulnerability to SIDS and cardiovascular complications later in life.
|
['Blood Pressure', 'Head', 'Heart Rate', 'Humans', 'Infant, Newborn', 'Infant, Premature', 'Polysomnography', 'Posture', 'Prevalence', 'Recovery of Function', 'Sleep', 'Sudden Infant Death', 'Time Factors']
| 19,895,423
|
[['E01.370.600.875.249', 'G09.330.380.076'], ['A01.456'], ['E01.370.600.875.500', 'G09.330.380.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703.520'], ['M01.060.703.520.520'], ['E01.370.520.625'], ['G11.427.695'], ['E05.318.308.985.525.750', 'N01.224.935.597.750', 'N06.850.505.400.975.525.750', 'N06.850.520.308.985.525.750'], ['G16.757'], ['F02.830.855', 'G11.561.803'], ['C23.550.260.322.625', 'C23.550.260.657.500'], ['G01.910.857']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Organisms [B]', 'Named Groups [M]', 'Health Care [N]', 'Psychiatry and Psychology [F]', 'Diseases [C]']
| 1
| 1
| 1
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Cyanotic limb in a newborn: a peculiar presentation of septic hip.
|
A 12-day-old infant presented with marked cyanosis of the right lower limb, mimicking vascular compromise. Physical examination, plain radiography, and laboratory evidence of systemic infection suggested septic arthritis of the hip. Pyoarthrosis was verified by needle aspiration and open drainage. Streptococcus pneumoniae was isolated. A cyanotic limb may be an atypical presentation for septic arthritis in the newborn.
|
['Arthritis, Infectious', 'Cyanosis', 'Female', 'Hip Joint', 'Humans', 'Infant, Newborn', 'Leg', 'Male', 'Pneumococcal Infections']
| 9,435,004
|
[['C01.100', 'C05.550.114.099'], ['C23.888.248'], ['A02.835.583.411'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703.520'], ['A01.378.610.500'], ['C01.150.252.410.890.670']]
|
['Diseases [C]', 'Anatomy [A]', 'Organisms [B]', 'Named Groups [M]']
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Sparse non-negative generalized PCA with applications to metabolomics.
|
MOTIVATION: Nuclear magnetic resonance (NMR) spectroscopy has been used to study mixtures of metabolites in biological samples. This technology produces a spectrum for each sample depicting the chemical shifts at which an unknown number of latent metabolites resonate. The interpretation of this data with common multivariate exploratory methods such as principal components analysis (PCA) is limited due to high-dimensionality, non-negativity of the underlying spectra and dependencies at adjacent chemical shifts.RESULTS: We develop a novel modification of PCA that is appropriate for analysis of NMR data, entitled Sparse Non-Negative Generalized PCA. This method yields interpretable principal components and loading vectors that select important features and directly account for both the non-negativity of the underlying spectra and dependencies at adjacent chemical shifts. Through the reanalysis of experimental NMR data on five purified neural cell types, we demonstrate the utility of our methods for dimension reduction, pattern recognition, sample exploration and feature selection. Our methods lead to the identification of novel metabolites that reflect the differences between these cell types.AVAILABILITY: www.stat.rice.edu/~gallen/software.html.CONTACT: gallen@rice.edu.SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
|
['Algorithms', 'Magnetic Resonance Spectroscopy', 'Metabolomics', 'Principal Component Analysis']
| 21,930,672
|
[['G17.035', 'L01.224.050'], ['E05.196.867.519'], ['H01.158.201.586', 'H01.158.273.180.599', 'H01.181.122.638'], ['E05.318.740.562']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]']
| 0
| 0
| 0
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
|
Association between helicobacter pylori infection and primary open angle glaucoma.
|
AIM: to compare the prevalence of Pylori infection in patients with primary open angle glaucoma (POAG) and control group with cataract.METHODS: This is a prospective case-control study. The participants were organized in two groups. First group (case) consisted of 35 patients with POAG and second group consisted of 35 age matched participants with cataract whose optic disk could be evaluated. Serum levels of anti H. pylori IgG antibody were evaluated with the method of ELISA.RESULTS: The seroprevalence of Pylori infection was 89.1 % (33 of 37) in patients with POAG and 59.5 % (25 of 42) in the control group. The difference was significant (P=0.008). The odds ratio for association between Pylori and POAG was 5.69 and the range of 95% confidence interval was from 1.58 to 20.50.CONCLUSION: This study suggests that Helicobacter Pylori infection might be associated with primary open angle glaucoma.
|
['Adult', 'Aged', 'Aged, 80 and over', 'Antibodies, Bacterial', 'Case-Control Studies', 'Enzyme-Linked Immunosorbent Assay', 'Female', 'Glaucoma, Open-Angle', 'Helicobacter Infections', 'Helicobacter pylori', 'Humans', 'Iran', 'Male', 'Middle Aged', 'Prevalence', 'Prospective Studies']
| 25,363,173
|
[['M01.060.116'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['D12.776.124.486.485.114.107', 'D12.776.124.790.651.114.125', 'D12.776.377.715.548.114.125'], ['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['E05.478.566.350.170', 'E05.478.566.380.360', 'E05.478.583.400.170', 'E05.601.470.350.170', 'E05.601.470.380.360'], ['C11.525.381.407'], ['C01.150.252.400.466'], ['B03.440.500.550', 'B03.660.150.235.500.250.550'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['Z01.252.245.500.350'], ['M01.060.116.630'], ['E05.318.308.985.525.750', 'N01.224.935.597.750', 'N06.850.505.400.975.525.750', 'N06.850.520.308.985.525.750'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Diseases [C]', 'Organisms [B]', 'Geographicals [Z]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Whole exome sequencing (WES) approach for diagnosing primary immunodeficiencies (PIDs) in a highly consanguineous community.
|
Primary immunodeficiencies (PIDs) are a heterogeneous group of monogenic inborn errors of immunity. The genetic causes of these diseases can be identified using whole exome sequencing (WES). Here, DNA samples from 106 patients with a clinical suspicion of PID were subjected to WES in order to test the diagnostic yield of this test in a highly consanguineous community. A likely genetic diagnosis was achieved in 70% of patients. Several factors were considered to possibly influence the diagnostic rate of WES among our cohort including early age, presence of consanguinity, family history suggestive of PID, the number of family members who underwent WES and the clinical phenotype of the patient. The highest diagnostic rate was in patients with combined immunodeficiency or with a syndrome. Notably, WES findings altered the clinical management in 39% (41/106) of patients in our cohort. Our findings support the use of WES as an important diagnostic tool in patients with suspected PID, especially in highly consanguineous communities.
|
['Adolescent', 'Adult', 'Autoimmune Diseases', 'Child', 'Child, Preschool', 'Clinical Decision-Making', 'Consanguinity', 'Disease Management', 'Female', 'Genotype', 'Hematopoietic Stem Cell Transplantation', 'Humans', 'Infant', 'Infant, Newborn', 'Inflammatory Bowel Diseases', 'Israel', 'Male', 'Mutation', 'Primary Immunodeficiency Diseases', 'Whole Exome Sequencing', 'Young Adult']
| 32,135,276
|
[['M01.060.057'], ['M01.060.116'], ['C20.111'], ['M01.060.406'], ['M01.060.406.448'], ['E01.055'], ['G05.090.403.180', 'G05.180'], ['N04.590.607'], ['G05.380'], ['E02.095.147.500.500.500', 'E04.936.225.687.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703'], ['M01.060.703.520'], ['C06.405.205.731', 'C06.405.469.432'], ['Z01.252.245.500.375'], ['G05.365.590'], ['C16.320.798', 'C20.673.795'], ['E05.393.760.700.825.500'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Organisms [B]', 'Geographicals [Z]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
[Reference intervals for peripheral blood lymphocyte subsets in healthy adults in Lima, Peru].
|
In order to establish the reference intervals (RIs) of peripheral blood lymphocyte subsets (PBL) in healthy adults in Lima (Peru), a cross-sectional study was conducted among blood donors taken in between 2011 and 2012. Based on the criteria obtained from the guidelines of the Clinical and Laboratory Standards Institute (CLSI C28-A3), 318 samples were processed, 61.9% (197/318) coming from male donors. For PBL count, a flow cytometer with a simple platform was used. The RIs are established for each PBL in adults based on sex with their respective reference limits and 90% confidence intervals. Differences were found in CD3+ percentage counts (p=0.001) and in CD3-CD56+ absolute (p=0.003) and percentage counts (p?0.001). The RIs found are different to those described in studies conducted in other countries due to the characteristics of the population and the study model.
|
['Adolescent', 'Adult', 'Cross-Sectional Studies', 'Female', 'Humans', 'Lymphocyte Count', 'Lymphocyte Subsets', 'Male', 'Middle Aged', 'Peru', 'Reference Values', 'Urban Population', 'Young Adult']
| 23,949,508
|
[['M01.060.057'], ['M01.060.116'], ['E05.318.372.500.875', 'N05.715.360.330.500.875', 'N06.850.520.450.500.875'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.225.500.195.107.595.500', 'E01.370.225.625.107.595.500', 'E05.200.500.195.107.595.500', 'E05.200.625.107.595.500', 'E05.242.195.107.595.500', 'G04.140.107.595.500', 'G09.188.105.595.500'], ['A11.118.637.555.567.550', 'A15.145.229.637.555.567.550', 'A15.382.490.555.567.550'], ['M01.060.116.630'], ['Z01.107.757.702'], ['E05.978.810'], ['N01.600.900'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Geographicals [Z]']
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Molecular interactions in gelatin/chitosan composite films.
|
Gelatin and chitosan were mixed at different mass ratios in solution forms, and the rheological properties of these film-forming solutions, upon cooling, were studied. The results indicate that the significant interactions between gelatin and chitosan promote the formation of multiple complexes, reflected by an increase in the storage modulus of gelatin solution. Furthermore, these molecular interactions hinder the formation of gelatin networks, consequently decreasing the storage modulus of polymer gels. Both hydrogen bonds and electrostatic interactions are formed between gelatin and chitosan, as evidenced by the shift of the amide-II bands of polymers. X-ray patterns of composite films indicate that the contents of triple helices decrease with increasing chitosan content. Only one glass transition temperature (Tg) was observed in composite films with different composition ratios, and it decreases gradually with an increase in chitosan proportion, indicating that gelatin and chitosan have good miscibility and form a wide range of blends.
|
['Chitosan', 'Gelatin', 'Polymers', 'Rheology']
| 28,554,645
|
[['D05.750.078.139.500', 'D09.698.211.500'], ['D12.776.860.476'], ['D05.750', 'D25.720', 'J01.637.051.720'], ['E05.830', 'H01.671.808']]
|
['Chemicals and Drugs [D]', 'Technology, Industry, and Agriculture [J]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]']
| 0
| 0
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
|
Nonlinear mezlocillin kinetics due to dose-dependent metabolism.
|
Dose-dependent kinetics of intravenous bolus-dose mezlocillin, a newly developed ureidopenicillin, have been reported by several investigators. In eight healthy subjects we examined renal, biliary, and total clearance at different steady-state levels. After infusion of doses of 22.4 +/- 1.5, 11.4 +/- 0.7, and 5.6 +/- 0.4 mg/min mean plasma steady-state levels of 88.4 +/- 15.9, 40.3 +/- 7.5, and 18.4 +/- 4.6 micrograms/ml were achieved. Metabolic clearance was estimated as the difference between total clearance and the sum of renal and biliary clearance, assuming that no other route of excretion plays an important role in the elimination of the drug. Total clearance varied between 355.7 +/- 59.3 and 286.5 +/- 54.6 ml/min when doses of 5.6 +/- 0.4 and 22.4 +/- 1.5 mg/min were given. Since neither renal clearance (137.6 +/- 32.6 ml/min) nor biliary clearance (98.6 +/- 42.5 ml/min) was shown to be dose dependent, nonlinear mezlocillin kinetics must be due to dose dependent metabolic clearance (108.4 +/- 26.2 versus 60.2 +/- 32.3 ml/min at 5.6 +/- 0.4 and 22.4 +/- 1.5 mg/min). The apparent Km was 27.5 micrograms/ml and apparent Vmax 4.3 mg/min. The apparent Km is so low that after therapeutic doses of mezlocillin the kinetics are nonlinear for several hours.
|
['Adult', 'Bile', 'Dose-Response Relationship, Drug', 'Drug Evaluation', 'Humans', 'Infusions, Parenteral', 'Kinetics', 'Male', 'Mathematics', 'Metabolic Clearance Rate', 'Mezlocillin', 'Penicillins']
| 6,220,858
|
[['M01.060.116'], ['A12.200.087'], ['G07.690.773.875', 'G07.690.936.500'], ['E05.290.625', 'E05.337.425'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.319.267.510'], ['G01.374.661', 'G02.111.490'], ['H01.548'], ['E01.370.225.843', 'E05.200.843', 'G03.490', 'G07.690.595', 'G07.690.725.513'], ['D02.065.589.099.750.750.050.500', 'D02.886.108.750.750.050.500', 'D03.633.100.300.750.750.050.500'], ['D02.065.589.099.750', 'D02.886.108.750', 'D03.633.100.300.750']]
|
['Named Groups [M]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Disciplines and Occupations [H]', 'Chemicals and Drugs [D]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 1
| 0
| 0
|
Digital Solutions for Informed Decision Making: An Academic-Community Partnership for the Development of a Prostate Cancer Decision Aid for African American Men.
|
African American (AA) men are significantly more likely to die of prostate cancer (PrCA) than other racial groups, and there is a critical need to identify strategies for providing information about PrCA screening and the importance of informed decision making (IDM). To assess whether a computer-based IDM intervention for PrCA screening would be appropriate for AA men, this formative evaluation study examined their (1) PrCA risk and screening knowledge; (2) decision-making processes for PrCA screening; (3) usage of, attitudes toward, and access to interactive communication technologies (ICTs); and (4) perceptions regarding a future, novel, computer-based PrCA education intervention. A purposive convenience sample of 39 AA men aged 37 to 66 years in the Southeastern United States was recruited through faith-based organizations to participate in one of six 90-minute focus groups and complete a 45-item descriptive survey. Participants were generally knowledgeable about PrCA. However, few engaged in IDM with their doctor and few were informed about the associated risks and uncertainties of PrCA screening. Most participants used ICTs on a daily basis for various purposes including health information seeking. Most participants were open to a novel, computer-based intervention if the system was easy to use and its animated avatars were culturally appropriate. Because study participants had low exposure to IDM for PrCA, but frequently used ICTs, IDM interventions using ICTs (e.g., computers) hold promise for AA men and should be explored for feasibility and effectiveness. These interventions should aim to increase PrCA screening knowledge and stress the importance of participating in IDM with doctors.
|
['African Americans', 'Community-Institutional Relations', 'Decision Making', 'Decision Support Techniques', 'Focus Groups', 'Health Knowledge, Attitudes, Practice', 'Humans', 'Information Services', 'Male', 'Mass Screening', 'Men', 'Middle Aged', 'Prostatic Neoplasms']
| 25,563,381
|
[['M01.686.508.100.100', 'M01.686.754.100'], ['N04.452.822.210'], ['F02.463.785.373'], ['E05.245', 'L01.313.500.750.190'], ['E05.318.308.112', 'N05.715.360.300.269', 'N06.850.520.308.112'], ['F01.100.150.500', 'N05.300.150.410'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['L01.453'], ['E01.370.500', 'E05.318.308.980.438.580', 'N02.421.726.233.443', 'N05.715.360.300.800.438.500', 'N06.850.520.308.980.438.580', 'N06.850.780.500'], ['M01.390'], ['M01.060.116.630'], ['C04.588.945.440.770', 'C12.294.260.750', 'C12.294.565.625', 'C12.758.409.750']]
|
['Named Groups [M]', 'Health Care [N]', 'Psychiatry and Psychology [F]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Information Science [L]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
| 0
|
A functional interaction between the p75 neurotrophin receptor interacting factors, TRAF6 and NRIF.
|
Neurotrophin signaling through the p75 receptor regulates apoptosis within the nervous system both during development and in response to injury. Whereas a number of p75 interacting factors have been identified, how these upstream factors function in a coordinated manner to mediate receptor signaling is still unclear. Here, we report a functional interaction between TRAF6 and the neurotrophin receptor interacting factor (NRIF), two proteins known to associate with the intracellular domain of the p75 neurotrophin receptor. The association between NRIF and TRAF6 was direct and occurred with both endogenous and ectopically expressed proteins. A KRAB repressor domain of NRIF and the carboxyl-terminal, receptor-binding region of TRAF6 were required for the interaction. Co-expression of TRAF6 increased the levels of NRIF protein and induced its nuclear translocation. Reciprocally, NRIF enhanced TRAF6-mediated activation of the c-Jun NH2-terminal kinase (JNK) by 3-fold, while only modestly increasing the stimulation of NF-kappaB. The expression of both NRIF and TRAF6 was required for reconstituting p75 activation of JNK in HEK293 cells, whereas NRIF mutants lacking the TRAF6 interaction domain were unable to substitute for the full-length protein in facilitating activation of the kinase. These results suggest that NRIF and TRAF6 functionally interact to facilitate neurotrophin signaling through the p75 receptor.
|
['Animals', 'Carrier Proteins', 'Cell Line', 'DNA-Binding Proteins', 'Humans', 'Intracellular Signaling Peptides and Proteins', 'Protein Binding', 'Protein Transport', 'Proteins', 'Receptor, Nerve Growth Factor', 'Receptors, Nerve Growth Factor', 'Signal Transduction', 'TNF Receptor-Associated Factor 6']
| 14,960,584
|
[['B01.050'], ['D12.776.157'], ['A11.251.210'], ['D12.776.260'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.644.360', 'D12.776.476'], ['G02.111.679', 'G03.808'], ['G03.143.700'], ['D12.776'], ['D12.776.543.750.750.400.550.500'], ['D12.776.543.750.750.400.550'], ['G02.111.820', 'G04.835'], ['D12.644.360.024.500.968', 'D12.776.157.057.500.968', 'D12.776.476.024.500.968']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Growth changes in the elastic properties of human tendon structures.
|
The purpose of this study was to investigate the growth changes in the elastic properties of human tendon structures. 9 younger boys (age 10.8 +/- 0.9 years, YBG), 9 elder boys (14.8 +/- 0.3 years, EBG), and 14 young adult men (24.7 +/- 1.6 years, ADG) volunteered to take part in the present study. Using a B-mode ultrasonic apparatus, the elongation of tendon and aponeurosis of vastus lateralis muscle (VL) was noninvasively measured in vivo, while subjects performed the extension of knee joint isometrically with force production levels from zero (relax) to maximal voluntary contraction (MVC) within 5 seconds. A curvilinear relationship was found between elongation of tendon structures (dL) and muscle force (Fm). This relationship consisted of two components, a steep initial change in length followed by a linear-region. The relationship between dL and Fm was fitted to a linear regression, and then the dL and dFm within 50 to 100% MVC was defined as compliance of tendon structures. The MVC force was the greatest in ADG and the lowest in YBG among the three groups. Significant age-related differences were found in compliance; 4.1 +/- 0.9 x 10(-2) mm/N for YBG, 2.9 +/- 1.1 x 10(-2) mm/N for EBG and 1.8 +/- 0.3 x 10(-2) mm/N for ADG. The dL/thigh length (TL) was significantly greater in YBG than in the other two groups above 0.35 MPa of Fm per muscle cross-sectional area (muscle stress). However, there was no significant difference between EBG and ADG in the relationship between dL/TL and muscle stress. The ratio of fascicle length to TL in YBG was significantly lower than those in the other two groups. These results suggest that the tendon structures in younger boys are more compliant than those in older boys and young men. The observed properties of tendon structures in the younger boys may play a role in protecting younger boys from athletic injuries associated with immature muscle-tendon complex.
|
['Adolescent', 'Adult', 'Age Factors', 'Child', 'Elasticity', 'Humans', 'Male', 'Muscle, Skeletal', 'Tendons', 'Tensile Strength']
| 11,281,617
|
[['M01.060.057'], ['M01.060.116'], ['N05.715.350.075', 'N06.850.490.250'], ['M01.060.406'], ['G01.374.590'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A02.633.567', 'A10.690.552.500'], ['A02.880'], ['G01.374.850']]
|
['Named Groups [M]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Anatomy [A]']
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Glucose metabolism after pancreas transplantation.
|
After pancreas transplantation in type-I diabetics the dependency on exogenous insulin usually vanishes rapidly. In about 50% of patients fasting and postprandial blood glucose concentrations are normal but by oral glucose tolerance tests the glucose metabolism is classified as normal in a minority. Some of the reasons for failure in obtaining good metabolic control are discussed. Attention is focused on the inevitable peripheral hyperinsulinemia and concomitant portal hypoinsulinemia related to the extra-portal administration of insulin.
|
['Diabetes Mellitus, Type 1', 'Glucose', 'Glucose Tolerance Test', 'Humans', 'Insulin', 'Pancreas Transplantation', 'Regional Blood Flow']
| 3,553,998
|
[['C18.452.394.750.124', 'C19.246.267', 'C20.111.327'], ['D09.947.875.359.448'], ['E01.370.225.124.100.355', 'E01.370.374.355', 'E05.200.124.100.355'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D06.472.699.587.200.500.625', 'D12.644.548.586.200.500.625'], ['E04.210.725', 'E04.936.450.650'], ['G09.330.100.780']]
|
['Diseases [C]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
The dissociation and separation of bovine adenohypophysial cells.
|
Bovine adenhypophysial tissue was dissociated by sequential enzymatic incubation in a continuous flow system. Dispersed cells separated into discrete fractions after centrifugation in isopycnic bovine serum albumin gradients. The dispersed and separated cells were prepared for microscopic identification and differential counts by centrifugal cytology. Radioimmunoassays for LH, FSH, TSH, and Prl were used to corroborate the differential counts and determine the homogeneity of the fractions. The thyrotrophs banded at an average density (rho) of 1.0417, the FSH-secretory cells at rho = 1.0597, the LH-secretory cells at rho = 1.0458, and the Prl-secretory cells at rho = 1.0126. A 7-16 fold enrichment of different cell populations was possible. In bovine hypophyses each hormone appears to be formed by specific cells: the average TSH concentrations of the thyrotrophs were 5.1 pg/cell for LH- and FSH concentration were 4.7 and 4.9 pg/cell for LH- and FSH-secreting cells, respectively. The average Prl concentration was 4.9 pg/cell for Prl-secreting cells.
|
['Animals', 'Cattle', 'Cell Separation', 'Centrifugation, Isopycnic', 'Female', 'Follicle Stimulating Hormone', 'Luteinizing Hormone', 'Male', 'Pituitary Gland, Anterior', 'Prolactin', 'Thyrotropin']
| 95,171
|
[]
|
[]
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Correlation between brachial artery flow-mediated dilation and endothelial microparticle levels for identifying endothelial dysfunction in children with Kawasaki disease.
|
BACKGROUND: We investigated vascular endothelial dysfunction by sonographic features of flow-mediated dilation (FMD) and circulating endothelial microparticles (EMPs) in Kawasaki disease (KD).METHODS: Twenty-eight patients with KD were prospectively grouped according to stage of disease: acute, subacute, and convalescent. In addition, 28 healthy children and 28 febrile children were selected as controls. And cases in the convalescent phase were divided into two subgroups: coronary artery lesion (CAL) and no coronary lesion (NCAL). CD144(+)/CD42b(-), CD62E(+), and CD105(+) EMPs were measured by flow cytometry; FMD was obtained by sonography.RESULTS: There were significant differences in FMD among the five groups. When compared with healthy controls, there were significantly greater numbers of CD144(+)/CD42b(-), CD62E(+), and CD105(+) EMPs and a higher proportion of CD62E(+) EMPs in KD patients. The proportions and numbers of CD144(+)/CD42b(-), CD62E(+), and CD105(+) EMPs in KD patients were not statistically different than in febrile controls. There were no significant differences in FMD and EMPs between the CAL and NCAL subgroups. There were significantly negative correlations between the values of FMD and EMPs in the three phases of KD.CONCLUSION: The increased levels of EMPs have significant correlation with decreased values of FMD, both of which may reflect endothelial dysfunction in child KD.
|
['Biomarkers', 'Brachial Artery', 'Case-Control Studies', 'Cell-Derived Microparticles', 'Child', 'Child, Preschool', 'China', 'Echocardiography', 'Endothelium, Vascular', 'Female', 'Flow Cytometry', 'Humans', 'Infant', 'Male', 'Mucocutaneous Lymph Node Syndrome', 'Prospective Studies', 'Regional Blood Flow', 'Statistics, Nonparametric', 'Ultrasonography', 'Vasodilation']
| 24,336,465
|
[['D23.101'], ['A07.015.114.139'], ['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['A11.284.295.588.500'], ['M01.060.406'], ['M01.060.406.448'], ['Z01.252.474.164'], ['E01.370.350.130.750', 'E01.370.350.850.220', 'E01.370.370.380.220'], ['A07.015.700.500', 'A10.272.491.355'], ['E01.370.225.500.363.342', 'E01.370.225.500.386.350', 'E05.196.712.516.600.240.350', 'E05.200.500.363.342', 'E05.200.500.386.350', 'E05.242.363.342', 'E05.242.386.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703'], ['C14.907.940.560', 'C15.604.560', 'C17.800.862.560'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['G09.330.100.780'], ['E05.318.740.995', 'N05.715.360.750.760', 'N06.850.520.830.995'], ['E01.370.350.850'], ['G09.330.380.928']]
|
['Chemicals and Drugs [D]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Named Groups [M]', 'Geographicals [Z]', 'Organisms [B]', 'Diseases [C]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Dipole localization and test-retest reliability of frequency and duration mismatch negativity generator processes.
|
The mismatch negativity (MMN) is an event related potential component elicited by changes in duration, frequency or intensity of the stimuli during repetitive series of equal standard stimuli. In the present study we compared duration and frequency MMN using dipole source analysis concerning both the test-retest reliability of MMN-amplitudes and the locations of the potential sources. Furthermore, the influence of attention for test-retest-reliability was studied. Therefore, two groups of healthy subjects were investigated with different attentional manipulations. Twenty-one healthy subjects had to perform a visual attention task during the recording and 21 healthy subjects had no additional task to perform. All subjects were studied twice with a time interval of 3 weeks. Test-retest reliability was sufficiently high for the frequency but slightly lower for the duration MMN. The locations of the frequency and duration MMN-dipoles were in the auditory cortex with a more anterior and caudal location for the frequency MMN-dipoles. The latter finding supports the hypothesis that the frequency and duration MMNs have separate neuronal generators.
|
['Adult', 'Artifacts', 'Brain', 'Brain Mapping', 'Electroencephalography', 'Electrophysiology', 'Evoked Potentials', 'Evoked Potentials, Visual', 'Humans', 'Middle Aged', 'Reproducibility of Results', 'Visual Perception']
| 9,358,949
|
[['M01.060.116'], ['E05.047'], ['A08.186.211'], ['E01.370.350.578.875.500', 'E01.370.376.537.625.500', 'E05.629.875.500'], ['E01.370.376.300', 'E01.370.405.245'], ['H01.158.344.528', 'H01.158.782.236'], ['G07.265.216.500', 'G11.561.200.500'], ['G07.265.216.500.425', 'G11.561.200.500.425', 'G14.330'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['E05.318.370.725', 'E05.337.851', 'N05.715.360.325.685', 'N06.850.520.445.725'], ['F02.463.593.932']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Disciplines and Occupations [H]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Health Care [N]', 'Psychiatry and Psychology [F]']
| 1
| 1
| 0
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 1
| 1
| 0
|
The anemia of heart failure.
|
Anemia is common in congestive heart failure (CHF) and is associated with an increased mortality and morbidity. The most likely causes of anemia are chronic kidney disease (CKD) and excessive cytokine production, both of which can cause depression of erythropoietin (EPO) production and bone marrow activity. The cytokines also induce iron deficiency by both reducing gastrointestinal iron absorption and iron release from iron stores located in the macrophages and hepatocytes. Iron deficiency can cause thrombocytosis which might also contribute to cardiovascular complications in both CHF and CKD and is partially reversible with iron treatment. Thus attempts to control this anemia will have to consider both the use of erythropoiesis-stimulating agents (ESA), such as EPO, as well as oral and, probably more importantly, intravenous (IV) iron. The many studies on anemia in CHF patients treated with ESA and oral or IV iron, and even with IV iron without ESA have up to now shown a quite consistent positive effect on hospitalization, fatigue, shortness of breath, quality of life, exercise capacity, and beta-natriuretic peptide reduction, in the absence of increased cardiovascular damage related to the therapy. Adequately powered long-term placebo-controlled studies of ESA and/or IV iron are currently being carried out and their results are eagerly awaited.
|
['Anemia', 'Erythropoietin', 'Heart Failure', 'Hemoglobins', 'Humans', 'Prevalence', 'Thrombocytosis']
| 19,907,148
|
[['C15.378.071'], ['D12.644.276.374.410.240.150', 'D12.776.395.240.150', 'D12.776.467.374.410.240.150', 'D23.529.374.410.240.150'], ['C14.280.434'], ['D12.776.124.400', 'D12.776.422.316.762'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.308.985.525.750', 'N01.224.935.597.750', 'N06.850.505.400.975.525.750', 'N06.850.520.308.985.525.750'], ['C15.378.140.860', 'C15.378.190.636.860']]
|
['Diseases [C]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Electropermeabilization of endocytotic vesicles in B16 F1 mouse melanoma cells.
|
It has been reported previously that electric pulses of sufficiently high voltage and short duration can permeabilize the membranes of various organelles inside living cells. In this article, we describe electropermeabilization of endocytotic vesicles in B16 F1 mouse melanoma cells. The cells were exposed to short, high-voltage electric pulses (from 1 to 20 pulses, 60 ns, 50 kV/cm, repetition frequency 1 kHz). We observed that 10 and 20 such pulses induced permeabilization of membranes of endocytotic vesicles, detected by release of lucifer yellow from the vesicles into the cytosol. Simultaneously, we detected uptake of propidium iodide through plasma membrane in the same cells. With higher number of pulses permeabilization of the membranes of endocytotic vesicles by pulses of given parameters is accompanied by permeabilization of plasma membrane. However, with lower number of pulses only permeabilization of the plasma membrane was detected.
|
['Animals', 'Cell Membrane', 'Cell Membrane Permeability', 'Electroporation', 'Isoquinolines', 'Melanoma, Experimental', 'Mice', 'Propidium', 'Transport Vesicles', 'Tumor Cells, Cultured']
| 20,361,267
|
[['B01.050'], ['A11.284.149'], ['G03.143.335', 'G04.175'], ['E05.200.500.454', 'E05.242.448', 'E05.301.500'], ['D03.633.100.531'], ['C04.557.465.625.650.510.525', 'C04.557.580.625.650.510.525', 'C04.557.665.510.525', 'C04.619.600', 'E05.598.500.496.937'], ['B01.050.150.900.649.313.992.635.505.500'], ['D03.633.300.633.700'], ['A11.284.430.214.190.875.190.880'], ['A11.251.860']]
|
['Organisms [B]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Cloning of two individual cDNAS encoding 9-cis-epoxycarotenoid dioxygenase from Gentiana lutea, their tissue-specific expression and physiological effect in transgenic tobacco.
|
Two 9-cis-epoxycarotenoid dioxygenase (NCED) cDNAs have been cloned from a petal library of Gentiana lutea. Both cDNAs carry a putative transit sequence for chloroplast import and differ mainly in their length and the 5'-flanking regions. GlNCED1 was evolutionary closely related to Arabidopsis thaliana NCED6 whereas GlNCED2 showed highest homology to tomato NCED1 and A. thaliana NCED3. The amounts of GlNCED2 transcript were below Northern detection in G. lutea. In contrast, GlNCED1 was specifically expressed at higher levels in developing flowers when petals start appearing. By genetic engineering of tobacco with coding regions of either gene under a constitutive promoter, their function was further analyzed. Although mRNA of both genes was detectable in the corresponding transgenic plants, a physiological effect was only found for GlNCED1 but not for GlNCED2. In germination experiments of GlNCED1 transgenic lines, delayed radicle formation and cotyledon appearance were observed. However, the transformants exhibited no improved tolerance against desiccation stress. In contrast to other plants with over-expressed NCEDs, prolonged delay of seed germination is the only abscisic-acid-related phenotypic effect in the GlNCED1 transgenic lines.
|
['Adaptation, Physiological', 'Dioxygenases', 'Flowers', 'Gene Expression Regulation, Plant', 'Gentiana', 'Germination', 'Oxygenases', 'Plant Proteins', 'Plants, Genetically Modified', 'Sequence Analysis, DNA', 'Tobacco', 'Transformation, Genetic', 'Water']
| 16,618,520
|
[['G07.025', 'G16.012.500'], ['D08.811.682.690.416'], ['A18.024.249.500'], ['G05.308.375'], ['B01.650.940.800.575.912.250.456.750.391'], ['G07.345.625.249', 'G15.357'], ['D08.811.682.690'], ['D12.776.765'], ['B01.650.520', 'B05.620.600'], ['E05.393.760.700'], ['B01.650.940.800.575.912.250.908.500.900'], ['G05.728.865'], ['D01.045.250.875', 'D01.248.497.158.459.650', 'D01.650.550.925']]
|
['Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Association of bone mineral density with hemoglobin and change in hemoglobin among older men and women: The Cardiovascular Health Study.
|
PURPOSE: Osteoblasts and their precursors support hematopoiesis in the bone marrow. We hypothesized that declines in Hgb levels are associated with bone mineral density (BMD).METHODS: The Cardiovascular Health Study is a prospective longitudinal study that enrolled 5888 community-dwelling adults aged >65 years and measured hemoglobin twice, in 1989-90 and 1992-93, as well as BMD by dual-energy X-ray absorptiometry (DXA) in 1994-95. In a subset of 1513 men and women with a Hgb in 1992-93 and BMD, we used linear regression to estimate associations of Hgb (per standard deviation (SD)) with total hip (TH), lumbar spine (LS) and total body (TB) BMD, and used Poisson regression to estimate associations of anemia (in 1992-93; Hgb <13 g/dL in men; <12 g/dL in women) with "low BMD" defined as T-score less than -1 at the TH. In 1277 participants with Hgb measured on average 2.9 years apart and BMD, we used linear regression to estimate the associations of annualized change in Hgb with TH, LS and TB BMD. All models included age, sex, study-site, race, smoking, alcohol use, weight, height, steroid use, physical activity score, self-reported health, previous cardiovascular disease and prior anti-fracture medication use.RESULTS: No significant association was observed between Hgb, measured a mean 2.2 years prior to BMD, and BMD at the TH and LS in men (TH beta = -0.60 [x 10-2 g/cm2per 1.1 g/dL Hgb], 95% CI: -1.88 to 0.68; LS beta = -1.69, 95% CI: -3.83 to 0.45) or women (TH beta = -0.49 [x 10-2 g/cm2per 1.3 g/dL Hgb], 95% CI: -1.57 to 0.59; LS beta = -0.40, 95% CI: -2.57 to 1.76). Anemia was not observed to be significantly associated with low BMD in men (RR = 0.99, 95% CI: 0.72-1.40) nor women (RR = 0.98, 95% CI: 0.82-1.17). The mean change in Hgb was a loss of 0.06 g/dL/year (SD = 0.32). Change in Hgb was not observed to be significantly associated with BMD in men (TH beta = -0.55[x 10-2 g/cm2per 1 g/dL annualized Hgb change], 95% CI: -4.28 to 3.19; LS beta = 0.63, 95% CI: -5.38 to 6.65) or women (TH beta = 0.92, 95% CI: -1.96 to 3.79; LS beta = -1.77, 95% CI: -7.52 to 3.98). No significant association was observed between anemia and low bone density by T-score in men and women.CONCLUSIONS: These findings support neither the hypothesis that low Hgb prior to bone density or decreases in Hgb are associated with bone density in older community-dwelling adults nor the use of Hgb level as a case-finding tool to prompt BMD measurement.
|
['Aged', 'Bone Density', 'Cardiovascular System', 'Female', 'Hemoglobins', 'Humans', 'Leukocyte Count', 'Male']
| 30,448,304
|
[['M01.060.116.100'], ['G11.427.100'], ['A07'], ['D12.776.124.400', 'D12.776.422.316.762'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.225.500.195.107.595', 'E01.370.225.625.107.595', 'E05.200.500.195.107.595', 'E05.200.625.107.595', 'E05.242.195.107.595', 'G04.140.107.595', 'G09.188.105.595']]
|
['Named Groups [M]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Early intervention for adolescents at high risk for the development of bipolar disorder: pilot study of Interpersonal and Social Rhythm Therapy (IPSRT).
|
Interpersonal and Social Rhythm Therapy (IPSRT) delays bipolar disorder (BP) recurrence in adults by stabilizing daily routines and sleep/wake cycles. Because adolescence is a key developmental stage for illness onset and altered social and sleep patterns, this period may prove optimal for intervention with adolescents at-risk for BP. We describe a treatment development trial of IPSRT for adolescents at-risk for BP by virtue of a positive family history. Adolescents with a first-degree relative with BP were evaluated for Axis I psychopathology via semistructured interview, and relatives' BP diagnoses were confirmed via record review. IPSRT consisted of 12 sessions delivered over 6 months. Outcome variables including sleep, mood symptoms, and functioning were assessed via clinician interview and self-/parent-report at pretreatment, 3 months, and posttreatment (6 months). Thirteen adolescents attended at least one IPSRT session. Half of the sample denied Axis I psychopathology at intake; the remainder met criteria for a range of internalizing and externalizing disorders. Families reported high satisfaction with IPSRT, yet, on average, participants attended about half of scheduled sessions. Missed sessions were primarily associated with parental BP illness severity. Data indicate significant change in select sleep/circadian patterns (i.e., less weekend sleeping in and oversleeping) with treatment. Preliminary data suggest the IPSRT focus on stabilizing daily rhythms and interpersonal relationships may be beneficial for adolescents at-risk for BP. Controlled trials with longitudinal follow-up are needed to examine whether early intervention for at-risk youth helps prevent or delay disorder.
|
['Adaptation, Psychological', 'Adolescent', 'Bipolar Disorder', 'Chronobiology Disorders', 'Early Medical Intervention', 'Family Conflict', 'Female', 'Genetic Predisposition to Disease', 'Humans', 'Internal-External Control', 'Interpersonal Relations', 'Life Change Events', 'Male', 'Patient Education as Topic', 'Personality Assessment', 'Pilot Projects', 'Psychotherapy', 'Risk Factors', 'Secondary Prevention', 'Social Adjustment', 'Social Stigma', 'United States']
| 24,377,402
|
[['F01.058'], ['M01.060.057'], ['F03.084.500'], ['C10.281'], ['N02.421.726.363'], ['F01.829.263.370.054', 'F01.829.401.142'], ['C23.550.291.687.500', 'G05.380.355'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F01.829.379'], ['F01.829.401'], ['F01.829.458.410'], ['I02.233.332.500', 'N02.421.726.407.680'], ['F04.513'], ['E05.318.372.750', 'E05.337.737', 'N05.715.360.330.720', 'N06.850.520.450.720'], ['F04.754'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['E02.897', 'N02.421.726.825', 'N06.850.780.750'], ['F01.145.813.621'], ['F01.145.813.840'], ['Z01.107.567.875']]
|
['Psychiatry and Psychology [F]', 'Named Groups [M]', 'Diseases [C]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Geographicals [Z]']
| 0
| 1
| 1
| 0
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 1
|
Neuronal injury in hippocampus with human immunodeficiency virus transactivating protein, Tat.
|
Patients with human immunodeficiency virus infection may develop a dementing illness. Using both in vitro and in vivo models, we investigated the susceptibility of the hippocampal formation to the Tat protein of human immunodeficiency virus. We also determined the pattern of hippocampal injury in patients with human immunodeficiency virus encephalitis. Following exposure of hippocampal slices to Tat, marked susceptibility of CA3 region with relative insensitivity of the CA1/2 region was observed. Injection of Tat into different regions of the rat hippocampus produced similar neuronal loss in both CA3 region and the dentate gyrus. In animals administered Tat, lesions were dose-dependent and immunohistochemical staining showed marked gliosis and loss of microtubule associated protein-2 in the affected areas at 3 days post-injection. Interestingly, synaptophysin staining was relatively preserved. In hippocampal tissue from patients with human immunodeficiency virus encephalitis, loss of microtubule-associated protein-2 staining was reduced in the molecular layer of the dentate gyrus. The results of our experiments demonstrate a unique pattern of hippocampal injury in organotypic culture and rats exposed to Tat. Our observations that patients with human immunodeficiency virus reveal a similar pattern of damage suggests that Tat protein may be pathophysiological relevant in human immunodeficiency virus encephalitis.
|
['Adolescent', 'Adult', 'Animals', 'Dose-Response Relationship, Drug', 'Gene Products, tat', 'Hippocampus', 'Humans', 'In Vitro Techniques', 'Male', 'Middle Aged', 'Neurons', 'Rats']
| 12,605,891
|
[['M01.060.057'], ['M01.060.116'], ['B01.050'], ['G07.690.773.875', 'G07.690.936.500'], ['D12.776.260.755.199', 'D12.776.930.900.199', 'D12.776.964.900.750.750', 'D12.776.964.925.984.400'], ['A08.186.211.180.405', 'A08.186.211.200.885.287.500.345'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.481'], ['M01.060.116.630'], ['A08.675', 'A11.671'], ['B01.050.150.900.649.313.992.635.505.700']]
|
['Named Groups [M]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
[Examination of circulating stem cells from the granulocyte-monocyte line in patients with Hodgkin's disease].
|
The formation of granulocyte-monocyte colonies by bone marrow cells cultured in a semiliquid environment is a widely applied technique of granulocytopoiesis examination. Applying this method the number of circulating in blood stem cells of the granulocyte-monocyte line (CFC-GM) has been assessed in 35 patients with Hodgkin's disease. In 12 patients the number of circulating in blood CFC-GM prior to the irradiation has been greater as compared with the norm accepted for our investigation. It has been the greatest in patients with nodular sclerosis type of Hodgkin's disease. In 25 patients smaller than accepted as normal number of circulating in the blood CFC-GM has been present particularly in patients with mixed cellularity type of Hodgkin's disease. Following the application of chemotherapy according to MOPP scheme during the first two weeks no circulating CFC-GM have been present in blood. The lowest number of stem cells in blood has been observed prior to IV and VI course of chemotherapy. The interruption of treatment for 8 weeks resulted in major increase of the number of circulating CFC-GM in the blood.
|
['Adult', 'Aged', 'Antineoplastic Combined Chemotherapy Protocols', 'Female', 'Granulocytes', 'Hematopoietic Stem Cells', 'Hodgkin Disease', 'Humans', 'Leukocyte Count', 'Male', 'Mechlorethamine', 'Middle Aged', 'Monocytes', 'Prednisone', 'Procarbazine', 'Vincristine']
| 2,356,144
|
[['M01.060.116'], ['M01.060.116.100'], ['E02.183.750.500', 'E02.319.077.500', 'E02.319.310.037'], ['A11.118.637.415', 'A11.148.350', 'A11.627.340', 'A15.145.229.637.415', 'A15.378.316.340', 'A15.382.490.315'], ['A11.148.378', 'A11.872.378', 'A15.378.316.378'], ['C04.557.386.355', 'C15.604.515.569.355', 'C20.683.515.761.355'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.225.500.195.107.595', 'E01.370.225.625.107.595', 'E05.200.500.195.107.595', 'E05.200.625.107.595', 'E05.242.195.107.595', 'G04.140.107.595', 'G09.188.105.595'], ['D02.455.526.728.650.529'], ['M01.060.116.630'], ['A11.118.637.555.652', 'A11.148.580', 'A11.627.624', 'A11.733.547', 'A15.145.229.637.555.652', 'A15.378.316.580', 'A15.382.490.555.652', 'A15.382.670.547', 'A15.382.680.547'], ['D04.210.500.745.432.719.702'], ['D02.065.277.727', 'D02.241.223.100.100.655', 'D02.455.426.559.389.127.085.655'], ['D03.132.436.681.827.817', 'D03.633.100.473.402.681.827.817', 'D03.633.100.496.500.500.681.827.817']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Diseases [C]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Quick-and-clean article figures with FigureJ.
|
We created FigureJ a new ImageJ plugin dedicated to scientific article figures preparation. Building a convincing figure is a demanding task that covers different steps ranging from content acquisition to figure assembly in editing software. Notions of image processing are required when it comes to even simple tasks such as cropping or resizing images and assembling them in a single figure. Scientific images are typically well handled in dedicated software but poorly supported in software used for laying out the final version of a figure for submission to review process.
|
['Image Processing, Computer-Assisted', 'Publishing', 'Software']
| 23,906,423
|
[['L01.224.308'], ['L01.737'], ['L01.224.900']]
|
['Information Science [L]']
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Simian varicella virus infects ganglia before rash in experimentally infected monkeys.
|
Monkeys experimentally infected with simian varicella virus (SVV) develop rash 10-14 days later. However, the route and the time of ganglionic infection are unknown. Using PCR, we analyzed DNA extracted from tissues of 13 monkeys 5 to 60 days after either intratracheal or intravenous inoculation with SVV. SVV DNA was detected in ganglia from four of five monkeys sacrificed 6 to 7 days after intratracheal inoculation. Further, analysis of ganglia from monkeys sacrificed at 10 days revealed that intravenous inoculation produced a higher proportion of SVV DNA-positive ganglia (63%) than that after intratracheal inoculation (13%), pointing to the role of hematogenous spread in ganglionic infection. Like other organs, monkey ganglia become infected with SVV before the appearance of rash.
|
['Animals', 'Chlorocebus aethiops', 'DNA, Viral', 'Exanthema', 'Ganglia', 'Herpesviridae Infections', 'Polymerase Chain Reaction', 'Varicellovirus']
| 11,145,914
|
[['B01.050'], ['B01.050.150.900.649.313.988.400.112.199.120.126.110'], ['D13.444.308.568'], ['C17.800.257'], ['A08.340'], ['C01.925.256.466'], ['E05.393.620.500'], ['B04.280.382.100.900']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Effect of a nurse-coordinated prevention programme on cardiovascular risk after an acute coronary syndrome: main results of the RESPONSE randomised trial.
|
OBJECTIVE: To quantify the impact of a practical, hospital-based nurse-coordinated prevention programme on cardiovascular risk, integrated into the routine clinical care of patients discharged after an acute coronary syndrome, as compared with usual care only.DESIGN: RESPONSE (Randomised Evaluation of Secondary Prevention by Outpatient Nurse SpEcialists) was a randomised clinical trial.SETTING: Multicentre trial in secondary and tertiary healthcare settings.PARTICIPANTS: 754 patients admitted for acute coronary syndrome.INTERVENTION: A nurse-coordinated prevention programme, consisting of four outpatient nurse clinic visits, focusing on healthy lifestyles, biometric risk factors and medication adherence, in addition to usual care.MAIN OUTCOME MEASURES: The main outcome was 10-year cardiovascular mortality risk as estimated by Systematic Coronary Risk Evaluation at 12 months follow-up. Secondary outcomes included Framingham Coronary Risk Score at 12 months, in addition to changes in individual risk factors. Risk factor control was classified as 'poor' if 0 to 3 factors were on target, 'fair' if 4 to 6 factors were on target, and 'good' if 7 to 9 were on target.RESULTS: The mean Systematic Coronary Risk Evaluation at 12 months was 4.4 per cent (SD 4.5) in the intervention group and 5.4 per cent (SD 6.2) in the control group (p=0.021), representing a 17.4% relative risk reduction. At 12 months, risk factor control classified as 'good' was achieved in 35% of patients in the intervention group compared with 25% in the control group (p=0.003). Attendance to the nurse-coordinated prevention programme was 92%. In the intervention group, 86 rehospitalisations were observed against 132 in the control group (relative risk reduction 34.8%, p=0.023).CONCLUSIONS: The nurse-coordinated hospital-based prevention programme in addition to usual care is a practical, yet effective method for reduction of cardiovascular risk in patients with coronary disease. Our data suggest that the counselling component of the programme may lead to a reduction in hospital readmissions.TRIAL REGISTRATION TRIALREGISTERNL IDENTIFIER: TC1290.
|
['Acute Coronary Syndrome', 'Aged', 'Ambulatory Care', 'Chi-Square Distribution', 'Female', 'Health Knowledge, Attitudes, Practice', 'Humans', 'Male', 'Medication Adherence', 'Middle Aged', 'Netherlands', 'Patient Education as Topic', 'Risk Assessment', 'Risk Factors', 'Risk Reduction Behavior', 'Secondary Prevention', 'Time Factors', 'Treatment Outcome']
| 23,813,851
|
[['C14.280.647.124', 'C14.907.585.124'], ['M01.060.116.100'], ['E02.760.106', 'N02.421.585.106'], ['E05.318.740.994.300', 'G17.820.300', 'N05.715.360.750.750.200', 'N06.850.520.830.994.300'], ['F01.100.150.500', 'N05.300.150.410'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F01.100.150.750.500.600.500', 'F01.145.488.887.500.600.500', 'N05.300.150.800.500.600.500'], ['M01.060.116.630'], ['Z01.542.651'], ['I02.233.332.500', 'N02.421.726.407.680'], ['E05.318.740.600.800.715', 'N04.452.871.715', 'N05.715.360.750.625.700.690', 'N06.850.505.715', 'N06.850.520.830.600.800.715'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['F01.145.699'], ['E02.897', 'N02.421.726.825', 'N06.850.780.750'], ['G01.910.857'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Diseases [C]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Psychiatry and Psychology [F]', 'Organisms [B]', 'Geographicals [Z]', 'Anthropology, Education, Sociology, and Social Phenomena [I]']
| 0
| 1
| 1
| 0
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 1
|
A homoallelic FECH mutation in a patient with both erythropoietic protoporphyria and palmar keratoderma.
|
BACKGROUND: Erythropoietic protoporphyria (EPP) is a hereditary disorder caused by the deficiency of ferrochelatase (FECH) in the haem biosynthetic pathway. In the majority of families, EPP is transmitted as a pseudodominant trait. Autosomal recessive form of EPP is found in only about 3% of the families.OBJECTIVES: In this study, we describe a 6-year-old boy who suffered from both EPP and palmar keratoderma.METHODS AND RESULTS: A novel homoallelic missense mutation (p.Ser318Tyr) was identified in the FECH gene. In addition, a region of homozygosity of approximately 6.8 Mb was observed in chromosome 18 of the patient by both microsatellite and SNP array. The parents of the patient, both of Palestinian (Jordanian) origin, were heterozygous for the S318Y mutation, although no history of consanguinity was known. Microsatellite genotyping identified a partial haplotype from each parent that corresponds to the region of homozygosity in the patient. Assuming S318Y is a founder mutation, the number of generations separating the two parents from their common ancestor from whom they inherited S318Y was estimated as 21.7 (95% CI 3.42–69.7).CONCLUSION: EPP was therefore inherited as an autosomal recessive trait in the family. This study confirms the association between palmar keratoderma and autosomal recessive EPP.
|
['Child', 'Family Health', 'Ferrochelatase', 'Genes, Recessive', 'Haplotypes', 'Homozygote', 'Humans', 'Keratoderma, Palmoplantar', 'Male', 'Models, Genetic', 'Mutation, Missense', 'Protoporphyria, Erythropoietic']
| 20,337,824
|
[['M01.060.406'], ['N01.400.300'], ['D08.811.520.500', 'D12.776.157.427.374.375.957', 'D12.776.556.579.374.375.617', 'D12.776.575.562'], ['G05.360.340.024.340.415', 'G05.420.325'], ['G05.380.360'], ['G05.380.554'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C16.320.850.475', 'C17.800.428.435', 'C17.800.827.475'], ['E05.599.395.397'], ['G05.365.590.650'], ['C06.552.830.812', 'C16.320.850.742.812', 'C17.800.827.742.812', 'C18.452.811.400.812']]
|
['Named Groups [M]', 'Health Care [N]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
[Treatment of fractures of long bones associated with extensive skin avulsion].
|
The work analyses the experience in the treatment of 46 patients with fractures of long tubular bones and extensive wounds with avulsion of the skin at the department of emergency traumatology from 1983 to 1988. The method of choice in the treatment of fractures of the long tubular bones in this category of patients is functionally-stable osteosynthesis mainly with external fixation apparatuses on a nail or rod basis. A rational succession of the manipulations for surgical debridement of the extensive wound was determined, which made the surgeon's work much easier and reduced the time needed for the operation. The skin defects in extensive wounds were repaired by Krasovitov's method in 16 patients, in 30 patients reimplantation of the skin was carried out by means of a split avulsed graft measuring 0.3-0.5 mm in thickness. A complex approach to the treatment of patients of bot groups was applied in the postoperative period, which improved the outcome of the treatment considerably. From comparative analysis of the immediate and late-term results of healing of the reimplanted grafts the authors conclude that dermatomic (0.4-0.5 mm) plastics with a split avulsed graft is preferable.
|
['Adolescent', 'Adult', 'Amputation, Traumatic', 'Arm Injuries', 'Body Surface Area', 'Child', 'Dermatologic Surgical Procedures', 'External Fixators', 'Female', 'Fracture Fixation, Internal', 'Fractures, Bone', 'Humans', 'Leg Injuries', 'Male', 'Middle Aged', 'Multiple Trauma', 'Replantation', 'Skin', 'Skin Transplantation', 'Suture Techniques']
| 1,469,873
|
[['M01.060.057'], ['M01.060.116'], ['C26.062'], ['C26.088'], ['E01.370.600.115.100.231', 'E05.041.124.231', 'G07.100.100.231'], ['M01.060.406'], ['E04.680.275'], ['E07.858.442.660.430', 'E07.858.690.725.430'], ['E04.555.300.300'], ['C26.404'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C26.558'], ['M01.060.116.630'], ['C26.640'], ['E04.936.494'], ['A17.815'], ['E02.095.147.725.700', 'E04.680.275.850', 'E04.936.580.700'], ['E04.987.775']]
|
['Named Groups [M]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Anatomy [A]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Mammareconstruction with skin-expander and silicone prostheses: 15 years' experience.
|
We report on 170 breasts that were reconstructed with Skin-Expander and silicone implants over a period of 15 years [1,2]. Reconstruction occurred primarily in only about one-third of the cases, since the information provided by the first doctor in charge regarding the possibilities of breast reconstruction was rather scanty. In cases treated with radiotherapy, reconstruction with Skin-Expander should not be excluded, but it is necessary to proceed with extreme caution and to give patients exact information. The complication rate is higher here than in cases which have not been exposed to radiotherapy. The final result depends directly on the primary surgery and the skin as well as the contralateral breast quality [3]. In most cases reconstruction with Skin-Expander and silicone implants meets the patients' desires: low-risk operation, fewer additional scars, and similar and improved breasts on both sides.
|
['Adult', 'Breast Implants', 'Breast Neoplasms', 'Female', 'Humans', 'Mammaplasty', 'Mastectomy', 'Middle Aged', 'Tissue Expansion', 'Tissue Expansion Devices']
| 12,140,703
|
[['M01.060.116'], ['E07.695.140'], ['C04.588.180', 'C17.800.090.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.218.565', 'E04.680.500'], ['E04.466'], ['M01.060.116.630'], ['E04.680.800'], ['E07.695.800', 'E07.858.886']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Mechanical unfolding of segment-swapped protein G dimer: results from replica exchange molecular dynamics simulations.
|
The protein G dimer (pdb code 1Q10) is a mutated dimeric form of the immunoglobulin-binding domain B1 of streptococcal protein G, in which the two monomeric units have swapped elements of their secondary structure. We have used replica exchange molecular dynamics simulations to study how this dimer responds to a mechanical force that pulls the N-terminus of one unit and the C-terminus of the other apart. We have further compared the mechanical response of the dimer to that of the protein G monomer. When the pulling force is low enough, the mechanical unfolding can be viewed as a thermally activated barrier crossing process. For each protein, we have computed the corresponding free energy barrier and its dependence on the pulling force. While the dimer is found to be less resistant to mechanical unfolding than its monomeric counterpart, the two proteins exhibit essentially the same mechanical unfolding mechanism involving separation of the terminal parallel strands. On the basis of our results, we speculate that the mechanical properties of natural adhesives, composites, fibers, and other materials may be optimized not only at a single molecule level but also at the mesoscopic level through the interactions among individual chains.
|
['Bacterial Proteins', 'Computer Simulation', 'Dimerization', 'Immunoglobulins', 'Models, Chemical', 'Protein Conformation', 'Protein Folding', 'Protein Structure, Secondary', 'Protein Subunits']
| 16,854,158
|
[['D12.776.097'], ['L01.224.160'], ['G02.206', 'G03.230'], ['D12.776.124.486.485', 'D12.776.124.790.651', 'D12.776.377.715.548'], ['E05.599.495'], ['G02.111.570.820.709'], ['G01.154.651', 'G02.111.688'], ['G02.111.570.820.709.600'], ['D12.776.813']]
|
['Chemicals and Drugs [D]', 'Information Science [L]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Plasma levels of angiostatin and endostatin remain unchanged for the first 3 weeks after colorectal cancer surgery.
|
INTRODUCTION: Angiostatin and endostatin are endogenous inhibitors of angiogenesis with anticancer effects. After minimally invasive colorectal resection (MICR), blood levels of the proangiogenic factors vascular endothelial growth factor (VEGF) and angiopoetin 2 (Ang-2) are elevated for 2-4 weeks. Also, postoperative human plasma from weeks 2 and 3 after MICR has been shown to stimulate endothelial cell proliferation and migration, which are critical to angiogenesis. This proangiogenic state may stimulate tumor growth early after MICR. Surgery's impact on angiostatin and endostatin is unknown. This study's purpose is to determine perioperative plasma levels of these two proteins in colorectal cancer (CRC) patients undergoing MICR.METHODS: Endostatin levels were assessed in 34 CRC patients and angiostatin levels in 30 CRC patients. Blood samples were taken preoperatively and on postoperative day (POD) 1 and 3 in all patients; in a subset, samples were taken between POD 7 and 20. The late samples were bundled into 7-day blocks (POD 7-13, POD 14-20) and considered as single time points. Angiostatin and endostatin plasma levels were determined via enzyme-linked immunosorbent assay (ELISA) in duplicate. Wilcoxon signed-rank test and Student's t test were used to analyze endostatin and angiostatin data, respectively. Significance was set at P<0.0125 (after Bonferroni correction).RESULTS: There was a significant decrease in median plasma endostatin levels on POD 1, which returned to the preoperative level by POD 3. There was no significant difference between pre- and postoperative plasma angiostatin levels.CONCLUSIONS: MICR has a very transient impact on plasma levels of endostatin and no impact on angiostatin during the first 21 days following surgery. Thus, angiostatin and endostatin do not likely contribute to or inhibit the persistent proangiogenic changes noted after MICR.
|
['Adenocarcinoma', 'Aged', 'Aged, 80 and over', 'Angiopoietin-2', 'Angiostatins', 'Colectomy', 'Colorectal Neoplasms', 'Endostatins', 'Enzyme-Linked Immunosorbent Assay', 'Female', 'Humans', 'Middle Aged', 'Minimally Invasive Surgical Procedures', 'Postoperative Period', 'Vascular Endothelial Growth Factor A']
| 21,181,203
|
[['C04.557.470.200.025'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['D12.644.276.100.100.200', 'D12.776.467.100.100.200', 'D23.529.100.100.200'], ['D08.622.610.500', 'D12.644.276.100.450.500', 'D12.776.124.790.223.580.500', 'D12.776.377.715.182.580.500', 'D12.776.467.100.450.500', 'D12.776.811.243.610.500', 'D23.529.100.450.500'], ['E04.210.219'], ['C04.588.274.476.411.307', 'C06.301.371.411.307', 'C06.405.249.411.307', 'C06.405.469.158.356', 'C06.405.469.491.307', 'C06.405.469.860.180'], ['D12.644.276.100.450.750', 'D12.776.467.100.450.750', 'D12.776.860.300.250.400.537.500', 'D23.529.100.450.750'], ['E05.478.566.350.170', 'E05.478.566.380.360', 'E05.478.583.400.170', 'E05.601.470.350.170', 'E05.601.470.380.360'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['E04.502'], ['E04.614.750', 'N02.421.585.753.750'], ['D12.644.276.100.800.200', 'D12.776.467.100.800.200', 'D23.529.100.800.200']]
|
['Diseases [C]', 'Named Groups [M]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Health Care [N]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
A comparison of psychological functioning in women at different stages of in vitro fertilization treatment using the mean affect adjective check list.
|
PURPOSE: The objectives were to identify the stage/s of IVF treatment where a woman is most vulnerable to psychological stress, and to assess the Mean Affect Adjective Check List (MAACL) as a measure of psychological functioning during IVF treatment, which has hitherto not been reported.METHODS: Thirty-seven women undergoing IVF treatment according to the long protocol of controlled ovarian stimulation completed the MAACL questionnaire at the following stages: (a) before treatment (visit 1), (b) before embryo transfer (visit 2), and (c) before pregnancy test (visit 3).RESULTS: Apart from anxiety scores for visit 2, the hostility, depression, and state anxiety scores for visit 3 were higher than the corresponding scores for visits 1 and 2 (P < 0.001). Anxiety scores for visits 2 and 3 were similar.CONCLUSIONS: Psychological counseling should be targeted at women after embryo transfer and leading up to the pregnancy test. The MAACL is a useful method for measuring psychological stress in women during IVF treatment.
|
['Age Factors', 'Anxiety', 'Depression', 'Embryo Transfer', 'Female', 'Fertilization in Vitro', 'Hostility', 'Humans', 'Infertility, Female', 'Psychometrics', 'Statistics, Nonparametric', 'Stress, Psychological', 'Surveys and Questionnaires']
| 11,209,535
|
[['N05.715.350.075', 'N06.850.490.250'], ['F01.470.132'], ['F01.145.126.350'], ['E02.875.800.500', 'E05.820.800.500'], ['E02.875.800.750', 'E05.820.800.750'], ['F01.470.596'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C13.351.500.365.700'], ['F04.711.780'], ['E05.318.740.995', 'N05.715.360.750.760', 'N06.850.520.830.995'], ['F01.145.126.990', 'F02.830.900'], ['E05.318.308.980', 'N05.715.360.300.800', 'N06.850.520.308.980']]
|
['Health Care [N]', 'Psychiatry and Psychology [F]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Surface tension and intrinsic amyloid fluorescence of serum and cerebrospinal fluid samples in Alzheimer?s disease.
|
AIM: Surface tension of biological fluids can be influenced by changes in oligomerization or aggregation of surfactant peptides or proteins. Amphiphilic peptides of amyloid-â or other amyloidogenic peptides/proteins display properties of surfactants, oligomerization and aggregation increase also their fluorescence intensity compared with native structures. Results/methodology: We estimated surface tension and native/ThioflavinT-based/intrinsic amyloid fluorescence intensity in serum and cerebrospinal fluid samples for their evalution as diagnostic biomarkers for Alzheimer?s disease (AD).DISCUSSION/CONCLUSION: Our results indicate that values of surface tension are not a suitable biomarker for AD. However, the ratio of ThioflavinT-based fluorescence to intrinsic amyloid fluorescence in cerebrospinal fluid appears to be an acceptable supportive diagnostic biomarker for AD (its sensitivity was 61.1%, and the specificity 70.8% when compared with aged controls).
|
['Adult', 'Aged', 'Alzheimer Disease', 'Amyloid', 'Biomarkers', 'Case-Control Studies', 'Female', 'Fluorescence', 'Follow-Up Studies', 'Humans', 'Male', 'Prognosis', 'Surface Tension']
| 30,888,831
|
[['M01.060.116'], ['M01.060.116.100'], ['C10.228.140.380.100', 'C10.574.945.249', 'F03.615.400.100'], ['D05.500.049', 'D12.776.049'], ['D23.101'], ['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['G01.358.500.505.650.665.500', 'G01.590.540.665.500'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.789'], ['G02.860.816']]
|
['Named Groups [M]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Rabies virus in insectivorous bats: implications of the diversity of the nucleoprotein and glycoprotein genes for molecular epidemiology.
|
Insectivorous bats are the main reservoirs of rabies virus (RABV) in various regions of the world. The aims of this study were to (a) establish genealogies for RABV strains from different species of Brazilian insectivorous bats based on the nucleoprotein (N) and glycoprotein (G) genes, (b) investigate specific RABV lineages associated with certain genera of bats and (c) identify molecular markers that can distinguish between these lineages. The genealogic analysis of N and G from 57 RABV strains revealed seven genus-specific clusters related to the insectivorous bats Myotis, Eptesicus, Nyctinomops, Molossus, Tadarida, Histiotus and Lasiurus. Molecular markers in the amino acid sequences were identified which were specific to the seven clusters. These results, which constitute a novel finding for this pathogen, show that there are at least seven independent epidemiological rabies cycles maintained by seven genera of insectivorous bats in Brazil.
|
['Animals', 'Antigens, Viral', 'Brazil', 'Chiroptera', 'Genetic Variation', 'Glycoproteins', 'Mice', 'Molecular Epidemiology', 'Nucleocapsid Proteins', 'Polymerase Chain Reaction', 'Rabies', 'Rabies virus', 'Sequence Analysis, DNA', 'Species Specificity', 'Viral Envelope Proteins']
| 20,609,456
|
[['B01.050'], ['D23.050.327'], ['Z01.107.757.176'], ['B01.050.150.900.649.313.937'], ['G05.365'], ['D09.400.430', 'D12.776.395'], ['B01.050.150.900.649.313.992.635.505.500'], ['E05.318.416', 'E05.393.522', 'H01.158.201.636.475.500', 'H01.158.273.343.595.475.500', 'H01.181.122.650.475.550', 'H02.403.720.500.300', 'N06.850.520.470'], ['D12.776.964.970.600'], ['E05.393.620.500'], ['C01.925.782.580.830.750'], ['B04.820.480.937.750.500.700'], ['E05.393.760.700'], ['G16.824'], ['D09.400.430.968', 'D12.776.395.550.993', 'D12.776.543.550.993', 'D12.776.964.970.880']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Geographicals [Z]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Health Care [N]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 1
|
Origin, structure, and role of background EEG activity. Part 1. Analytic amplitude.
|
OBJECTIVE: To explain the neural mechanisms of spontaneous EEG by measuring the spatiotemporal patterns of synchrony among beta-gamma oscillations during perception.METHODS: EEGs were measured from 8 x 8 (5.6 x 5.6 mm2) arrays fixed on the surfaces of primary sensory areas in rabbits that were trained to discriminate visual, auditory or tactile conditioned stimuli (CSs) eliciting conditioned responses (CRs). EEG preprocessing was by (i) bandpass filtering to extract the beta-gamma range (deleting theta-alpha); (ii) low-pass spatial filtering (not high-pass Laplacians used for localization), (iii) spatial averaging (not time averaging used for evoked potentials), and (iv) close spacing of 64 electrodes for simultaneous recording in each area (not sampling single signals from several areas); (v) novel algorithms were devised to measure synchrony and spatial pattern stability by calculating variances among patterns in 64-space derived from the 8 x 8 arrays (not by fitting equivalent dipoles). These methodological differences are crucial for the proposed new perspective on EEG.RESULTS: Spatial patterns of beta-gamma EEG emerged following sudden jumps in cortical activity called 'state transitions'. Each transition began with an abrupt phase re-setting to a new value on every channel, followed sequentially by re-synchronization, spatial pattern stabilization, and a dramatic increase in pattern amplitude. State transitions recurred at varying intervals in the theta range. A novel parameter was devised to estimate the perceptual information in the beta-gamma EEG, which disclosed 2-4 patterns with high information content in the CS-CR interval on each trial; each began with a state transition and lasted approximately 0.1 s.CONCLUSIONS: The function of each primary sensory neocortex was discontinuous; discrete spatial patterns occurred in frames like those in cinema. The frames before and after the CS-CR interval had low content.SIGNIFICANCE: Derivation and interpretation of unit data in studies of perception might benefit from using multichannel EEG recordings to define distinctive epochs that are demarcated by state transitions of neocortical dynamics in the CS-CR intervals, particularly in consideration of the possibility that EEG may reveal recurring episodes of exchange and sharing of perceptual information among multiple sensory cortices. Simultaneously recorded, multichannel beta-gamma EEG might assist in the interpretation of images derived by fMRI, since high beta-gamma EEG amplitudes imply high rates of energy utilization. The spatial pattern intermittency provides a tag to distinguish gamma bursts from contaminating EMG activity in scalp recording in order to establish beta-gamma recording as a standard clinical tool. Finally, EEG cannot fail to have a major impact on brain theory.
|
['Acoustic Stimulation', 'Algorithms', 'Animals', 'Conditioning, Psychological', 'Discrimination, Psychological', 'Electroencephalography', 'Entropy', 'Models, Neurological', 'Neocortex', 'Photic Stimulation', 'Rabbits', 'Touch']
| 15,294,210
|
[['E02.037', 'E02.190.888.030', 'E05.723.136'], ['G17.035', 'L01.224.050'], ['B01.050'], ['F02.463.425.179'], ['F02.463.593.257'], ['E01.370.376.300', 'E01.370.405.245'], ['G01.906.345'], ['E05.599.395.642'], ['A08.186.211.200.885.287.500.420'], ['E05.723.729'], ['B01.050.150.900.649.313.968.700'], ['F02.830.816.850', 'G11.561.790.850']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Organisms [B]', 'Psychiatry and Psychology [F]', 'Anatomy [A]']
| 1
| 1
| 0
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Involvement of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and p53 in neuronal apoptosis: evidence that GAPDH is upregulated by p53.
|
We recently reported that cytosine arabinoside (AraC)-induced apoptosis of cerebellar neurons involves the overexpression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The present study was undertaken to investigate whether p53 and/or Bax overexpression participates in the AraC-induced apoptosis of cerebellar granule cells and, if so, the relationship between p53 induction and GAPDH overexpression in these cells. AraC-induced apoptosis of cerebellar granule cells was preceded by an increase in levels of p53 mRNA and protein detected between 1 and 8 hr after treatment. The mRNA level for a p53 target gene, Bax, was also increased. The increase in GAPDH mRNA lasted longer than that of either p53 or Bax, and the level of GAPDH protein in the particulate fraction increased after induction of GAPDH mRNA. The antisense oligonucleotide to p53 protected granule cells from AraC-induced chromatin condensation, internucleosomal cleavage, and apoptotic death. The inhibition of p53 expression by the p53 antisense oligonucleotide not only blocked the expression of Bax but also partially suppressed the increased GAPDH mRNA and protein levels. Conversely, the suppression of GAPDH expression and subsequent attenuation of apoptosis of granule cells by GAPDH antisense oligonucleotide did not influence the expression of p53 or Bax. Cerebellar granule cells prepared from p53 knock-out mice were resistant to AraC toxicity, and the p53 gene knock-out suppressed AraC-upregulated GAPDH expression. Moreover, infection of PC12 cells with an adenoviral vector containing p53 gene dramatically increased GAPDH expression and triggered cell apoptosis. These results suggest that AraC-induced apoptosis of cerebellar granule cells involves the expression of both GAPDH and p53 and that, similar to Bax, GAPDH is upregulated by p53 after exposure to the apoptotic insult.
|
['Animals', 'Apoptosis', 'Cerebellum', 'Cytarabine', 'Gene Expression Regulation', 'Gene Expression Regulation, Enzymologic', 'Genes, p53', 'Glyceraldehyde-3-Phosphate Dehydrogenases', 'Mice', 'Mice, Knockout', 'Neurons', 'Oligodeoxyribonucleotides, Antisense', 'PC12 Cells', 'Proto-Oncogene Proteins', 'Proto-Oncogene Proteins c-bcl-2', 'Rats', 'Rats, Sprague-Dawley', 'Recombinant Proteins', 'Transfection', 'Tumor Suppressor Protein p53', 'bcl-2-Associated X Protein']
| 10,531,467
|
[['B01.050'], ['G04.146.954.035'], ['A08.186.211.132.810.428.200'], ['D03.383.742.680.245.453', 'D13.570.065.300', 'D13.570.685.245.453'], ['G05.308'], ['G05.308.320'], ['G05.360.340.024.340.375.249.385', 'G05.360.340.024.340.415.400.385'], ['D08.811.682.657.163.750'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.136.500.500', 'B01.050.150.900.649.313.992.635.505.500.550.455', 'B01.050.150.900.649.313.992.635.505.500.800.500'], ['A08.675', 'A11.671'], ['D13.150.200.640', 'D13.150.480.640', 'D13.444.308.150.640', 'D13.444.600.150.200.640', 'D13.444.600.150.640.640', 'D13.695.578.424.480.640', 'D27.720.470.530.600.150.200.640', 'D27.720.470.530.600.150.640.640'], ['A11.251.210.190.750', 'A11.251.860.180.750', 'A11.299.500'], ['D12.776.624.664.700'], ['D12.644.360.075.718', 'D12.776.476.075.718', 'D12.776.624.664.700.169'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.750'], ['D12.776.828'], ['E05.393.350.810', 'G05.728.860'], ['D12.776.157.687.650', 'D12.776.260.820', 'D12.776.624.776.775', 'D12.776.660.720.650', 'D12.776.744.845'], ['D12.644.360.075.718.400', 'D12.776.476.075.718.400']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[Lipopolysaccharide, TNFá, IL-6, dexamethasone, and insulin increase the expression of GPR54 in the MCF7 breasr cancer cell line].
|
OBJECTIVE: To investigate the effects of different concentrations of lipopolysaccharide (LPS), tumor necrosis factor á (TNFá), interleukin-6 (IL-6), dexamethasone (Dex), and insulin on the mRNA and protein expressions of GPR54 in the MCF7 cell line in vitro.METHODS: MCF7 breasr cancer cells were cultured and treated with different concentrations of LPS (10 and 20 µg/ml), TNFá (20 and 100 ng/ml), IL-6 (10 and 20 ng/ml), Dex (10(-6) and 10(-7) mol/L), and insulin (0.01 and 0.1 IU/L). Those treated with culture fluid only served as controls. The mRNA and protein expressions of GPR54 were measured by real-time PCR and Western blot, respectively, after 6, 24, 48, and 72 hours of treatment.RESULTS: Compared with the blank con- trol, LPS (10 and 20 µg/ml), TNFá (20 and 100 ng/ml), IL-6 (10 and 20 ng/ml), Dex (10(-6) and 10(-7) mol/L), and insulin (0.01 and 0.1 IU/L) significantly increased the expressions of GPR54 mRNA (P < 0.05) and protein (P < 0.05).CONCLUSION: LPS, TNFá, IL-6, Dex, and insulin evidently increase the expression of GPR54 in the MCF7 cell line, indicating their influence on the function of gonads by regulating the GPR54 level.
|
['Blotting, Western', 'Dexamethasone', 'Glucocorticoids', 'Gonads', 'Humans', 'Hypoglycemic Agents', 'Insulin', 'Interleukin-6', 'Lipopolysaccharides', 'MCF-7 Cells', 'RNA, Messenger', 'Real-Time Polymerase Chain Reaction', 'Receptors, G-Protein-Coupled', 'Receptors, Kisspeptin-1', 'Time Factors', 'Tumor Necrosis Factor-alpha']
| 26,333,218
|
[['E05.196.401.143', 'E05.301.300.096', 'E05.478.566.320.200', 'E05.601.262', 'E05.601.470.320.200'], ['D04.210.500.745.432.769.344', 'D04.210.500.908.238'], ['D06.472.040.543', 'D27.505.696.399.472.488'], ['A05.360.576', 'A06.300.312'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D27.505.696.422'], ['D06.472.699.587.200.500.625', 'D12.644.548.586.200.500.625'], ['D12.644.276.374.465.224', 'D12.776.467.374.465.202', 'D23.529.374.465.224'], ['D09.400.500', 'D09.698.718.450', 'D10.494', 'D23.050.161.616.525', 'D23.946.123.329.500'], ['A11.251.210.190.630'], ['D13.444.735.544'], ['E05.393.620.500.706'], ['D12.776.543.750.695'], ['D12.776.543.750.695.022', 'D12.776.543.750.750.500'], ['G01.910.857'], ['D12.644.276.374.500.800', 'D12.644.276.374.750.626', 'D12.776.124.900', 'D12.776.395.930', 'D12.776.467.374.500.800', 'D12.776.467.374.750.626', 'D23.529.374.500.800', 'D23.529.374.750.626']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Organisms [B]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Urinary nerve growth factor levels could be a biomarker for overactive bladder symptom: a meta-analysis.
|
To examine whether urinary tract nerve growth factor (uNGF) could be a biomarker for overactive bladder (OAB) symptom, we conducted a comprehensive meta-analysis of 8 case-control studies. In all the studies considered, patients with OAB symptom had a higher uNGF level compared to healthy people. In addition, patients had a significantly lower uNGF level after successful treatment. In the subgroup analysis, we found that patients with OAB-wet symptom had a higher uNGF level than patients with OAB-dry symptom. However, no significant difference was found between patients with OAB symptom and patients with interstitial cystitis/painful bladder syndrome (IC/PBS) symptom in uNGF/Cr levels. In conclusion, uNGF level could be a useful biomarker for the diagnosis of OAB, a possible biomarker for differentiation between OAB subtypes (wet or dry), and a predictive biomarker for a specific treatment, but it cannot be used as the urinary biomarker for the differential diagnosis of IC/PBS and OAB.
|
['Biomarkers', 'Case-Control Studies', 'Humans', 'Nerve Growth Factor', 'Urinary Bladder, Overactive']
| 24,782,215
|
[['D23.101'], ['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.644.276.860.437', 'D12.776.467.860.437', 'D12.776.631.600.437', 'D23.529.850.437'], ['C12.777.829.866', 'C13.351.968.829.813', 'C23.888.942.343.780']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Locus coeruleus lesions potentiate neurotoxic effects of MPTP in dopaminergic neurons of the substantia nigra.
|
The observation that Parkinson's disease (PD) is associated with locus coeruleus (LC) noradrenergic neuronal degeneration suggests that the LC noradrenergic system may be involved in the pathogenesis and natural progression of the destruction of the substantia nigra (SN) dopaminergic neurons in Parkinson's disease. The relationship of these two systems was examined by injection of subtoxic doses of MPTP into unilateral LC 6-hydroxydopamine (6-OHDA) lesioned mice. A significant loss of dopaminergic cells was only found in the SN on the side of the LC lesions. These results suggest that the LC may have protective effects on SN dopaminergic neurons.
|
['Animals', 'Base Sequence', 'Dopamine', 'Immunohistochemistry', 'In Situ Hybridization', 'Locus Coeruleus', 'MPTP Poisoning', 'Mice', 'Mice, Inbred BALB C', 'Molecular Sequence Data', 'Norepinephrine', 'Oxidopamine', 'RNA, Messenger', 'Substantia Nigra', 'Tyrosine 3-Monooxygenase']
| 7,704,612
|
[['B01.050'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['D02.092.211.215.406', 'D02.092.311.342', 'D02.455.426.559.389.657.166.175.342'], ['E01.370.225.500.607.512', 'E01.370.225.750.551.512', 'E05.200.500.607.512', 'E05.200.750.551.512', 'E05.478.583', 'H01.158.100.656.234.512', 'H01.158.201.344.512', 'H01.158.201.486.512', 'H01.181.122.573.512', 'H01.181.122.605.512'], ['E01.370.225.500.620.670.325', 'E01.370.225.750.600.670.325', 'E05.200.500.620.670.325', 'E05.200.750.600.670.325', 'E05.393.661.475'], ['A08.186.211.132.659.473', 'A08.186.211.132.810.428.600.650.437'], ['C10.228.140.079.862.800.300', 'C10.228.662.600.700.250', 'C10.720.606', 'C25.723.705.400'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.338', 'B01.050.150.900.649.313.992.635.505.500.400.338'], ['L01.453.245.667'], ['D02.033.100.291.502', 'D02.092.063.480', 'D02.092.211.215.746', 'D02.092.311.830', 'D02.455.426.559.389.657.166.175.830'], ['D02.092.311.342.478.650', 'D02.455.426.559.389.657.166.175.342.478.650'], ['D13.444.735.544'], ['A08.186.211.132.659.413.656'], ['D08.811.682.690.708.923', 'D12.776.556.579.374.925']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Anatomy [A]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
|
Induction of N-methyl-D-asparate receptor mediated c-fos protein in the rat brain by incomplete ischaemia.
|
The expression of c-fos protein was examined by means of immunocytochemistry in the rat brain following incomplete ischaemia, to elucidate the molecular mechanisms of post-ischaemic neuronal death and of the modulated neurotransmission of surviving neurons. Incomplete ischaemia was produced by permanent unilateral or bilateral common carotid artery (CCA) occlusion. After 1 h of unilateral occlusion, the level of c-fos protein-like nuclear immunoreactivity increased in cortical neurons ipsilateral to the insult, especially in cingulate and piriform cortices. The reactivity peaked at 3-6 h, and was undetectable after 3 days. A number of scattered immunostained neurons in the ipsilateral subiculum, CA 1 and dentate gyrus became visible after 1 day. The effect reached a peak between 1-3 days, then returned to basal levels by 7 days. Bilateral CCA occlusion showed a similar distribution of immunoreactivity, but on both hemispheres. Immunoreactive neurons were more numerous and intensely stained but more transient. The induction of c-fos was completely blocked or reduced by treatment with MK-801. Our results suggest that c-fos expression after CCA occlusion is NMDA receptor mediated, and that it has a specific role in neurons after ischaemic insult.
|
['Animals', 'Brain', 'Carotid Artery, Common', 'Dizocilpine Maleate', 'Ischemic Attack, Transient', 'Ligation', 'Male', 'Proto-Oncogene Proteins c-fos', 'Rats', 'Receptors, N-Methyl-D-Aspartate']
| 7,829,168
|
[['B01.050'], ['A08.186.211'], ['A07.015.114.186.200'], ['D02.455.426.559.847.181.384.380', 'D04.615.181.384.380'], ['C10.228.140.300.150.836', 'C14.907.253.092.836'], ['E04.426'], ['D12.776.260.108.765', 'D12.776.624.664.700.179', 'D12.776.660.760', 'D12.776.930.127.765'], ['B01.050.150.900.649.313.992.635.505.700'], ['D12.776.157.530.400.400.500.500', 'D12.776.543.550.450.500.200.500', 'D12.776.543.585.400.500.200.500', 'D12.776.543.750.720.200.450.400.500']]
|
['Organisms [B]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Immunophenotypic and genotypic characterization of B-cell chronic lymphocytic leukemia patients from northern Italy.
|
BACKGROUND AND METHODS: Immunoglobulin (Ig) gene expression and rearrangements provide information about cell lineage, clonality and differentiation of neoplastic lymphoid cells. We performed flow cytometry and Southern blot analysis in 25 patients with chronic lymphocytic leukemia (CLL) from the Po River delta region (Northern Italy), in order to correlate surface immunological phenotype and heavy and light chain gene rearrangements with clinical stages.RESULTS: In these 25 CLL cases, as in the larger group of 165, k-positive (k+) patients were more frequent than lambda-positive (lambda +) ones by a ratio of 59:41, with a predominance of lambda + in stage II (46:54) and of K+ in stage IV (77:23). However, the survival behavior of lambda + and k+ patients was not statistically different. Ig gene rearrangements were found in all patients in accordance with the immunophenotype. When Ig gene analysis was performed on two different occasions in 7 of the 8 who received chemotherapy, there was an increase in the percentage of the germline band. This variation was inversely related to changes in WBC count. In the treated subject who showed a reduction of both WBC count and the germline, there was rapid disease progression and short survival.CONCLUSIONS: Analysis of Ig gene rearrangements in our CLL patients demonstrated: i) the monoclonality of neoplastic cells; ii) no correlation between rearrangements and clinical stages, and iii) that this analysis may be a useful tool in evaluating biological behavior in selected CLL cases showing a discrepancy between hematological data and clinical response to therapy.
|
['Clone Cells', 'DNA, Neoplasm', 'Female', 'Gene Expression Regulation, Neoplastic', 'Gene Rearrangement, B-Lymphocyte', 'Genes, Immunoglobulin', 'Genotype', 'Humans', 'Immunoglobulin kappa-Chains', 'Immunoglobulin lambda-Chains', 'Immunophenotyping', 'Italy', 'Leukemia, Lymphocytic, Chronic, B-Cell', 'Male', 'Neoplasm Proteins', 'Neoplastic Stem Cells', 'Receptors, Antigen, B-Cell']
| 8,491,418
|
[['A11.251.353'], ['D13.444.308.425'], ['G05.308.370'], ['G05.344.401', 'G12.500.274'], ['G05.360.340.024.340.335', 'G12.500.299'], ['G05.380'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.776.124.486.485.705.750.530', 'D12.776.124.790.651.705.750.530', 'D12.776.377.715.548.705.750.530'], ['D12.776.124.486.485.705.750.550', 'D12.776.124.790.651.705.750.550', 'D12.776.377.715.548.705.750.550'], ['E01.370.225.812.447', 'E05.200.812.447', 'E05.478.594.450'], ['Z01.542.489'], ['C04.557.337.428.080.125', 'C15.604.515.560.080.125', 'C20.683.515.528.080.125'], ['D12.776.624'], ['A11.872.650'], ['D12.776.124.790.651.950', 'D12.776.377.715.548.950', 'D12.776.543.750.705.816.821']]
|
['Anatomy [A]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Geographicals [Z]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
|
[Maternity, sterility and reproduction technology].
|
The idea of maternity--and paternity as well--reaches far beyond the field of biological reproduction inasmuch as it implies a subject's desire, besides being regulated by the symbolic order: Language, myths, patterns and values of a given culture. From this point of view, infertility cannot be considered as a mere somatic illness, but as a human problem implying psychological and social aspects that require a search for solutions unlikely to be reduced to a sole medical operation. The development of the new reproductive technology is in keeping with the increasing medicalization of human lives, human sexuality, and human bodies.
|
['Adult', 'Female', 'Fertilization in Vitro', 'Humans', 'Infertility, Female', 'Infertility, Male', 'Insemination, Artificial', 'Male', 'Maternal Behavior', 'Ovulation Induction', 'Paternal Behavior', 'Pregnancy', 'Reproductive Techniques']
| 7,992,630
|
[['M01.060.116'], ['E02.875.800.750', 'E05.820.800.750'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C13.351.500.365.700'], ['C12.294.365.700'], ['E02.875.800.937', 'E05.820.800.937', 'G08.686.784.363.492'], ['F01.829.263.370.215'], ['E02.875.800.984', 'E05.820.800.984'], ['F01.829.263.370.330'], ['G08.686.784.769'], ['E02.875', 'E05.820']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Psychiatry and Psychology [F]']
| 0
| 1
| 1
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
[Therapeutic activity of Physioquens in the treatment of certain gynecological conditions (author's transl)].
|
The new hormonal preparation Physioquens was administered during the study to 181 women during 1.634 cycles and has proved to be very effective in the treatment of various gynaecological disturbances. A definitive reduction has been observed in premenstrual and pre and postmenopausal complaints, dysmenorrhoea, amenorrhoea, intermenstrual pain and endometriosis. Physiquens is also a perfect regulatory device of the cycle in the case of menorrhagia, metrorrhagia and irregular menstrual cycles connected with a hormonal insufficiency. The normophasic treatment with Physioquens is made up of the cyclical administration of a tablet of 0.050 mg ethinyloestradiol for the first 7 days, followed by a tablet of 1 mg lynestrenol and 0.050 mg ethinyloestradiol for the next 15 days. Because of its effectiveness and good tolerance both clinically and biologically Physioquens can be considered an important new step in the treatment of gynaecological problems.
|
['Drug Evaluation', 'Ethinyl Estradiol', 'Female', 'Humans', 'Lynestrenol', 'Menopause', 'Menstruation Disturbances', 'Pregnancy']
| 1,213,240
|
[['E05.290.625', 'E05.337.425'], ['D04.210.500.668.651.568.291', 'D06.472.334.851.437.968.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D04.210.500.668.651.693.494'], ['G08.686.157.500', 'G08.686.841.249.500'], ['C23.550.568'], ['G08.686.784.769']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Asymmetric effects of cooler and warmer winters on beech phenology last beyond spring.
|
In temperate trees, the timings of plant growth onset and cessation affect biogeochemical cycles, water, and energy balance. Currently, phenological studies largely focus on specific phenophases and on their responses to warming. How differently spring phenology responds to the warming and cooling, and affects the subsequent phases, has not been yet investigated in trees. Here, we exposed saplings of Fagus sylvatica L. to warmer and cooler climate during the winter 2013-2014 by conducting a reciprocal transplant experiment between two elevations (1,340 vs. 371 m a.s.l., ca. 6°C difference) in the Swiss Jura mountains. To test the legacy effects of earlier or later budburst on the budset timing, saplings were moved back to their original elevation shortly after the occurrence of budburst in spring 2014. One degree decrease in air temperature in winter/spring resulted in a delay of 10.9 days in budburst dates, whereas one degree of warming advanced the date by 8.8 days. Interestingly, we also found an asymmetric effect of the warmer winter vs. cooler winter on the budset timing in late summer. Budset of saplings that experienced a cooler winter was delayed by 31 days compared to the control, whereas it was delayed by only 10 days in saplings that experienced a warmer winter. Budburst timing in 2015 was not significantly impacted by the artificial advance or delay of the budburst timing in 2014, indicating that the legacy effects of the different phenophases might be reset during each winter. Adapting phenological models to the whole annual phenological cycle, and considering the different response to cooling and warming, would improve predictions of tree phenology under future climate warming conditions.
|
['Climate', 'Climate Change', 'Cold Temperature', 'Fagus', 'Hot Temperature', 'Plant Development', 'Seasons', 'Trees']
| 28,464,396
|
[['G16.500.275.071', 'N06.230.300.100.250'], ['G16.500.175.374'], ['G01.906.595.272', 'G16.500.275.063.725.710.300', 'G16.500.750.775.710.300', 'N06.230.300.100.725.154', 'N06.230.300.100.725.710.300'], ['B01.650.940.800.575.912.250.859.750.300.249'], ['G01.906.595.543', 'G16.500.275.063.725.710.380', 'G16.500.750.775.710.380', 'N06.230.300.100.725.232', 'N06.230.300.100.725.710.380'], ['G07.345.625', 'G15.589'], ['G01.910.645.661', 'G16.500.275.071.590', 'N06.230.300.100.250.525'], ['B01.650.915']]
|
['Phenomena and Processes [G]', 'Health Care [N]', 'Organisms [B]']
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Working conditions and health in Central America: a survey of 12,024 workers in six countries.
|
OBJECTIVE: To describe the survey methodology and initial general findings of the first Central American Survey of Working Conditions and Health.METHODS: A representative sample of 12,024 workers was interviewed at home in Costa Rica, El Salvador, Guatemala, Honduras, Nicaragua and Panama. Questionnaire items addressed worker demographics, employment conditions, occupational risk factors and self-perceived health.RESULTS: Overall, self-employment (37%) is the most frequent type of employment, 8% of employees lack a work contract and 74% of the workforce is not covered by social security. These percentages are higher in Guatemala, Honduras and El Salvador, and lower in Costa Rica, Panama and Nicaragua. A third of the workforce works more than 48 h per week, regardless of gender; this is similar across countries. Women and men report frequent or usual exposures to high ambient temperature (16% and 25%, respectively), dangerous tools and machinery (10%, 24%), work on slippery surfaces (10%, 23%), breathing chemicals (12.1%, 18%), handling toxic substances (5%, 12.1%), heavy loads (6%, 20%) and repetitive movements (43%, 49%). Two-thirds of the workforce perceive their health as being good or very good, and slightly more than half reports having good mental health.CONCLUSIONS: The survey offers, for the first time, comparable data on the work and health status of workers in the formal and informal economy in the six Spanish-speaking Central American countries, based on representative national samples. This provides a benchmark for future monitoring of employment and working conditions across countries.
|
['Accidents, Occupational', 'Adult', 'Central America', 'Contracts', 'Cumulative Trauma Disorders', 'Data Collection', 'Developing Countries', 'Employment', 'Female', 'Hazardous Substances', 'Health', 'Hot Temperature', 'Humans', 'Interviews as Topic', 'Male', 'Middle Aged', 'Occupational Exposure', 'Social Security', 'Stress, Mechanical', 'Surface Properties', 'Surveys and Questionnaires', 'Workload']
| 24,652,231
|
[['N06.850.135.240'], ['M01.060.116'], ['Z01.107.169'], ['I01.880.604.583.090', 'N03.706.535.241'], ['C26.844.150'], ['E05.318.308', 'L01.399.250', 'N05.715.360.300', 'N06.850.520.308'], ['I01.615.500.300'], ['N01.824.245'], ['D27.888.426'], ['N01.400'], ['G01.906.595.543', 'G16.500.275.063.725.710.380', 'G16.500.750.775.710.380', 'N06.230.300.100.725.232', 'N06.230.300.100.725.710.380'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.308.420', 'L01.399.250.520', 'N05.715.360.300.400', 'N06.850.520.308.420'], ['M01.060.116.630'], ['N06.850.460.350.600'], ['N03.219.521.346.506.849', 'N03.219.521.576.823'], ['G01.374.835'], ['G02.860'], ['E05.318.308.980', 'N05.715.360.300.800', 'N06.850.520.308.980'], ['I03.946.225.500', 'N04.452.677.650.500']]
|
['Health Care [N]', 'Named Groups [M]', 'Geographicals [Z]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Information Science [L]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 1
| 1
| 1
| 1
|
Preliminary studies on DNA retardation by MutS applied to the detection of point mutations in clinical samples.
|
MutS ability to bind DNA mismatches was applied to the detection of point mutations in PCR products. MutS recognized mismatches from single up to five nucleotides and retarded the electrophoretic migration of mismatched DNA. The electrophoretic detection of insertions/deletions above three nucleotides is also possible without MutS, thanks to the DNA mobility shift caused by the presence of large insertion/deletion loops in the heteroduplex DNA. Thus, the method enables the search for a broad range of mutations: from single up to several nucleotides. The mobility shift assays were carried out in polyacrylamide gels stained with SYBR-Gold. One assay required 50-200 ng of PCR product and 1-3 microg of Thermus thermophilus his6-MutS protein. The advantages of this approach are: the small amounts of DNA required for the examination, simple and fast staining, no demand for PCR product purification, no labelling and radioisotopes required. The method was tested in the detection of cancer predisposing mutations in RET, hMSH2, hMLH1, BRCA1, BRCA2 and NBS1 genes. The approach appears to be promising in screening for unknown point mutations.
|
['Cell Cycle Proteins', 'DNA', 'DNA-Binding Proteins', 'Genes, BRCA1', 'Multiple Endocrine Neoplasia Type 2b', 'MutS Homolog 2 Protein', 'Nuclear Proteins', 'Oncogene Proteins', 'Point Mutation', 'Proto-Oncogene Proteins', 'Proto-Oncogene Proteins c-ret', 'Receptor Protein-Tyrosine Kinases']
| 15,680,407
|
[['D12.776.167'], ['D13.444.308'], ['D12.776.260'], ['G05.360.340.024.340.375.249.100', 'G05.360.340.024.340.415.400.100'], ['C04.588.322.400.510', 'C04.651.600.510', 'C04.700.630.510', 'C16.320.700.630.510', 'C19.344.400.510'], ['D08.811.074.844.750', 'D08.811.277.040.025.292.750', 'D12.776.260.556.750', 'D12.776.624.664.700.130'], ['D12.776.660'], ['D12.776.624.664'], ['G05.365.590.675'], ['D12.776.624.664.700'], ['D08.811.913.696.620.682.725.400.087', 'D12.776.395.550.200.188.500', 'D12.776.543.131.500', 'D12.776.543.750.630.217', 'D12.776.624.664.700.194'], ['D08.811.913.696.620.682.725.400', 'D12.776.543.750.630']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Diseases [C]']
| 0
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Identification of a novel collagen type ?-binding protein from Streptococcus suis serotype 2.
|
Streptococcus suis, a major pathogen of pigs, is an emerging zoonotic agent that causes meningitis and septic shock. cbp40 is a putative virulent gene that has been identified using suppression subtractive hybridization performed on the virulent S. suis serotype 2 strain HA9801 and the avirulent S. suis serotype 2 strain T15. Based on predicted protein features showing a shared conserved domain with the collagen-binding protein Cna of Staphylococcus aureus, Cbp40 is likely to function as a direct mediator of collagen adhesion. Here, the cbp40 gene was cloned and the recombinant protein purified. Western blotting using swine convalescent sera confirmed its role as an immunogenic protein. Collagen binding activity could be detected by western affinity blot and ELISA. Conversely, deletion of the cbp40 gene reduced bacterial adhesion to HEp-2 cells, capacity for biofilm formation, and virulence in a zebrafish infection model. The response of the bEnd.3 cell line to infection with the S. suis serotype 2 strain ZY05719 and the cbp40-knockout strain was evaluated using gene expression arrays. The differentially expressed genes were involved in inflammatory and immune responses, leukocyte adhesion and heterophilic cell adhesion. Collectively, these data suggest that Cbp40 plays an important role as an extracellular matrix adhesion protein that interacts with host cells during infection.
|
['Animals', 'Bacterial Adhesion', 'Bacterial Proteins', 'Biofilms', 'Cloning, Molecular', 'Epithelial Cells', 'Gene Expression Regulation, Bacterial', 'Hep G2 Cells', 'Humans', 'Streptococcal Infections', 'Streptococcus suis', 'Swine', 'Zebrafish']
| 23,465,548
|
[['B01.050'], ['G06.099.050'], ['D12.776.097'], ['A20.593', 'G06.120'], ['E05.393.220'], ['A11.436'], ['G05.308.300'], ['A11.251.860.180.432', 'A11.436.348.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C01.150.252.410.890'], ['B03.353.750.737.872.750', 'B03.510.400.800.872.750', 'B03.510.550.737.872.750'], ['B01.050.150.900.649.313.500.880'], ['B01.050.150.900.493.200.244.828']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Protein-protein and DNA-protein interactions affect the activity of lymphoid-specific IFN regulatory factors.
|
IFN regulatory factors (IRFs) constitute a family of transcription factors that are involved in IFN signaling and the development and differentiation of the immune system. Targeted gene disruption studies in mice assigned their primary role to the immune system. Two lymphoid-specific IRF members, IFN consensus sequence binding protein (ICSBP) and IRF-4, bind target DNA with greater efficiency following interaction with two transcription factors, PU.1 and E47, leading to transcriptional synergy. PU.1 and E47 are essential for proper differentiation and maturation of lymphoid cells. In addition, ICSBP interacts with two IRF members, IRF-1 and IRF-2, which also have central roles in the regulation of cell-mediated immunity. Previously, we identified a region in ICSBP, termed the IRF association domain (IAD), that is conserved in all IRFs (excluding IRF-1 and IRF-2) and is essential for its interactions with other IRF proteins. Here we show that the IAD is an independent module used by ICSBP and IRF-4 for protein-protein interactions. In addition, an IAD of IRF-2 (IAD2), necessary for interaction with ICSBP, was identified and found to be conserved in IRF-1. The IAD2 shares similar characteristics with the PEST domain that is essential for the interaction of PU.1 with IRF-4. We also show that the ICSBP DNA binding domain is indispensable for the formation of DNA binding heterocomplexes and transcriptional activity. Therefore, our results shed light on the molecular mechanisms that affect IRF activities in the immune system via discrete functional domains.
|
['3T3 Cells', 'Amino Acid Motifs', 'Amino Acid Sequence', 'Animals', 'Consensus Sequence', 'DNA', 'DNA-Binding Proteins', 'Interferon Regulatory Factor-2', 'Interferon Regulatory Factors', 'Interferons', 'Mice', 'Molecular Sequence Data', 'Protein Structure, Tertiary', 'Proto-Oncogene Proteins', 'Repressor Proteins', 'TCF Transcription Factors', 'Trans-Activators', 'Transcription Factor 7-Like 1 Protein', 'Transcription Factors', 'Transcription, Genetic']
| 10,586,038
|
[['A11.251.210.100', 'A11.329.228.100'], ['G02.111.570.820.709.275.500', 'G02.111.570.820.709.600.500'], ['G02.111.570.060', 'L01.453.245.667.060'], ['B01.050'], ['G02.111.570.580.175'], ['D13.444.308'], ['D12.776.260'], ['D12.644.360.024.302.249', 'D12.776.157.057.050.249', 'D12.776.260.504.249', 'D12.776.476.024.385.249', 'D12.776.930.332.249'], ['D12.644.360.024.302', 'D12.776.157.057.050', 'D12.776.260.504', 'D12.776.476.024.385', 'D12.776.930.332'], ['D12.644.276.374.440', 'D12.776.467.374.440', 'D23.529.374.440'], ['B01.050.150.900.649.313.992.635.505.500'], ['L01.453.245.667'], ['G02.111.570.820.709.610'], ['D12.776.624.664.700'], ['D12.776.260.703', 'D12.776.930.780'], ['D12.776.260.730', 'D12.776.660.235.400.800', 'D12.776.664.235.400.800', 'D12.776.930.875'], ['D12.776.260.755', 'D12.776.930.900', 'D12.776.964.925.984'], ['D12.776.260.730.812', 'D12.776.660.235.400.800.812', 'D12.776.664.235.400.800.812', 'D12.776.930.875.812'], ['D12.776.930'], ['G02.111.873', 'G05.297.700']]
|
['Anatomy [A]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Organisms [B]', 'Chemicals and Drugs [D]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Association between adiposity and inflammatory markers in maternal and fetal blood in a group of Mexican pregnant women.
|
In the present pilot study, we evaluated the effect of maternal adiposity on the plasma concentration of adipocytokines in pregnant women and their newborns. Twenty patients with term gestations without labour were initially selected by pregestational BMI and then classified into two study groups (n 10 each), according to their median value of adiposity (total body fat). Concentrations of TNF-á, IL-1â, IL-6, leptin and adiponectin in plasma of maternal peripheral blood and fetal cord blood were measured and correlated to maternal adiposity. Maternal adiposity showed a significant negative correlation with fetal adiponectin (r - 0·587, P = 0·01) and IL-6 (r - 0·466, P = 0·05), a significant positive correlation with maternal leptin (r 0·527, P = 0·02) and no correlation with TNF-á or IL-1â. Adiponectin was higher in fetal plasma than in maternal plasma (P = 0·043), but significantly lower in newborns from women with high adiposity than in newborns from women with low adiposity (P = 0·040). Our results suggest that fetuses from obese women may be less able to control inflammation, due to lower circulating anti-inflammatory adipocytokines, which could limit their optimal development or even increase the risk of abortion or preterm labour.
|
['Adipokines', 'Adiponectin', 'Adiposity', 'Adult', 'Biomarkers', 'Birth Weight', 'Cytokines', 'Female', 'Fetal Blood', 'Gestational Age', 'Humans', 'Infant, Newborn', 'Inflammation', 'Leptin', 'Male', 'Maternal Age', 'Mexico', 'Pilot Projects', 'Pregnancy', 'Young Adult']
| 20,650,016
|
[['D06.472.699.042', 'D12.644.276.024', 'D12.644.548.011', 'D12.776.467.024', 'D23.529.024'], ['D06.472.699.042.249', 'D12.644.276.024.249', 'D12.644.548.011.249', 'D12.776.467.024.249', 'D23.529.024.249'], ['E01.370.600.115.100.062.500', 'G02.111.130.134.500', 'G03.180.134.500', 'G07.100.049.134.500'], ['M01.060.116'], ['D23.101'], ['C23.888.144.186', 'E01.370.600.115.100.160.120.186', 'E05.041.124.160.750.149', 'G07.100.100.160.120.186', 'G07.345.249.314.120.186'], ['D12.644.276.374', 'D12.776.467.374', 'D23.529.374'], ['A12.207.152.200', 'A15.145.300', 'A16.378.200'], ['G07.345.500.325.235.968', 'G08.686.320'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703.520'], ['C23.550.470'], ['D06.472.699.042.500', 'D12.644.276.024.500', 'D12.644.548.011.500', 'D12.776.467.024.500', 'D23.529.024.500'], ['G08.686.560', 'N05.715.350.075.550', 'N06.850.490.250.550'], ['Z01.107.567.589'], ['E05.318.372.750', 'E05.337.737', 'N05.715.360.330.720', 'N06.850.520.450.720'], ['G08.686.784.769'], ['M01.060.116.815']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Named Groups [M]', 'Diseases [C]', 'Anatomy [A]', 'Organisms [B]', 'Health Care [N]', 'Geographicals [Z]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Baroreceptor reflexes in human hypertension.
|
We studied the control of arterial pressure by the carotid sinus baroreceptors in 35 hypertensive humans, using a variable pressure neck chamber to alter carotid sinus transmural pressure in a graded fashion. The results were compared with those obtained from 11 normotensives. As in normotensives, reduction in carotid transmural pressure caused a linearly related pressor response and vice versa. However, whereas in normotensives the pressure response was greater than the depressor, the reverse was the case in hypertensives. Furthermore, the pressor response decreased and the depressor response increased progressively with an increase in severity of the hypertension. Thus while in normotensives the carotid baroreflex is more effective in protecting against hypotension, in hypertensives the antihypertensive function of the reflex is favored. Similar differences between hypertensives and normotensives were found with respect to the carotid baroreceptor control of heart rate. In eight hypertensives, reflex changes in heart rate also were studied by injection of phenylephrine and trinitroglycerine to vary not only carotid baroreceptor activity, but also activity of extracarotid baroreceptors. The results were compared with results of similar studies on eight normotensives. These comparisons suggest that, whereas the carotid baroreceptor reflex remains active in hypertension, reflexes stemming from extracarotid baroreceptor areas are much diminished.
|
['Adult', 'Aged', 'Blood Pressure', 'Carotid Arteries', 'Female', 'Heart Rate', 'Hemodynamics', 'Humans', 'Hypertension', 'Male', 'Middle Aged', 'Nitroglycerin', 'Phenylephrine', 'Pressoreceptors', 'Reflex']
| 97,032
|
[['M01.060.116'], ['M01.060.116.100'], ['E01.370.600.875.249', 'G09.330.380.076'], ['A07.015.114.186'], ['E01.370.600.875.500', 'G09.330.380.500'], ['G09.330.380'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C14.907.489'], ['M01.060.116.630'], ['D02.640.636'], ['D02.033.100.291.617', 'D02.092.063.291.617'], ['A08.675.650.915.750.750', 'A08.800.050.800.900.700', 'A08.800.950.750.750', 'A11.671.650.915.750.750'], ['E01.370.376.550.650', 'E01.370.600.550.650', 'F02.830.702', 'G11.561.731']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Organisms [B]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Psychiatry and Psychology [F]']
| 1
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Comparison of a multipoint identity-by-descent method with parametric multipoint linkage analysis for mapping quantitative traits.
|
We previously developed a method of partitioning genetic variance of a quantitative trait to loci in specific chromosomal regions. In this paper, we compare this method--multipoint IBD (identical by descent) method (MIM)--with parametric multipoint linkage analysis (MLINK). A simulation study was performed comparing the methods for the major-locus, mixed, and two-locus models. The criterion for comparisons between MIM and MLINK was the average lod score from multiple replicates of simulated data sets. The effect of gene frequency, dominance, model misspecification, marker spacing, and informativeness are also considered in a smaller set of simulations. Within the context of the models examined, the MIM approach was found to be comparable in power with parametric multipoint linkage analysis when (a) parental data are unknown, (b) the effect of the major locus is small and there is additional genetic variation, or (c) the parameters of the major-locus model are misspecified. The performance of the MIM method relative to MLINK was markedly lower when the allele frequency at the trait locus was .2 versus .5, particularly for the case when parental data were assumed to be known. Dominance at the trait major locus, as well as marker spacing and heterozygosity, did not appear to have a large effect on the ELOD comparisons.
|
['Chromosome Mapping', 'Computer Simulation', 'Confounding Factors, Epidemiologic', 'Gene Frequency', 'Genes, Dominant', 'Genetic Linkage', 'Genetic Markers', 'Genetic Variation', 'Humans', 'Likelihood Functions', 'Lod Score', 'Models, Genetic', 'Parents']
| 1,539,596
|
[['E05.393.183'], ['L01.224.160'], ['N05.715.350.240', 'N06.850.490.718'], ['G05.330'], ['G05.360.340.024.340.240', 'G05.420.320'], ['G05.348'], ['D23.101.387', 'G05.695.450'], ['G05.365'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.740.500.475', 'E05.318.740.600.400', 'E05.599.835.500', 'N05.715.360.750.530.450', 'N05.715.360.750.625.450', 'N06.850.520.830.500.475', 'N06.850.520.830.600.400'], ['G05.348.750'], ['E05.599.395.397'], ['F01.829.263.500.320', 'I01.880.853.150.500.340', 'M01.620']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Information Science [L]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Psychiatry and Psychology [F]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Named Groups [M]']
| 0
| 1
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 1
| 1
| 0
|
Quantitative NMRI and NMRS identify augmented disease progression after loss of ambulation in forearms of boys with Duchenne muscular dystrophy.
|
Quantitative NMRI and (31)P NMRS indices are reported in the forearms of 24 patients with Duchenne muscular dystrophy (DMD) (6-18 years, 14 non-ambulant) amenable to exon 53 skipping therapy and in 12 age-matched male controls (CONT). Examinations carried out at 3 T comprised multi-slice 17-echo measurements of muscle water T2 and heterogeneity, three-point Dixon imaging of fat fraction in flexor and extensor muscles (FLEX, EXT), and non-localised spectroscopy of phosphate metabolites. We studied four imaging indices, eight metabolic ratios combining ATP, phosphocreatine, phosphomonoesters and phosphodiesters, the cytosolic inorganic phosphate (Pia ) and an alkaline (Pib) pool present in dystrophic muscle, and average pH. All indices differed between DMD and CONT, except for muscle water T2 . Measurements were outside the 95th percentile of age-matched CONT values in over 65% of cases for percentage fat signal (%F), and in 78-100% of cases for all spectroscopic indices. T2 was elevated in one-third of FLEX measurements, whereas %pixels > 39 ms and T2 heterogeneity were abnormal in one-half of the examinations. The FLEX muscles had higher fat infiltration and T2 than EXT muscle groups. All indices, except pH, correlated with patient age, although the correlation was negative for T2 . However, in non-ambulant patients, the correlation with years since loss of ambulation was stronger than the correlation with age, and the slope of evolution per year was steeper after loss of ambulation. All indices except Pi/gATP differed between ambulant and non-ambulant patients; however, T2 and %pixels > 39 ms were highest in ambulant patients, possibly owing to the greater extent of inflammatory processes earlier in the disease. All other indices were worse in non-ambulant subjects. Quantitative measurements obtained from patients at different disease stages covered a broad range of abnormalities that evolved with the disease, and metabolic indices were up to 10-fold above normal from the onset, thus establishing a variety of potential markers for future therapy.
|
['Adenosine Triphosphate', 'Adolescent', 'Child', 'Disease Progression', 'Forearm', 'Humans', 'Magnetic Resonance Imaging', 'Magnetic Resonance Spectroscopy', 'Male', 'Muscular Dystrophy, Duchenne']
| 26,215,733
|
[['D03.633.100.759.646.138.236', 'D13.695.667.138.236', 'D13.695.827.068.236'], ['M01.060.057'], ['M01.060.406'], ['C23.550.291.656'], ['A01.378.800.585'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.350.825.500'], ['E05.196.867.519'], ['C05.651.534.500.300', 'C10.668.491.175.500.300', 'C16.320.322.562', 'C16.320.577.300']]
|
['Chemicals and Drugs [D]', 'Named Groups [M]', 'Diseases [C]', 'Anatomy [A]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Correction of intermetatarsal angle in hallux valgus using small suture button device.
|
BACKGROUND: Hallux valgus is a common foot ailment causing pain and disability, and correction of the intermetatarsal angle (IMA) deformity is often accomplished using a first metatarsal distal or proximal osteotomy. These osteotomies can be technically challenging and may lead to complications such as loss of fixation, shortening of the first metatarsal, avascular necrosis, malunion, and nonunion. Endobuttons (Mini TightRope device) provide an alternative to first metatarsal osteotomies for correction of the IMA. The purpose of this preliminary study was to determine the short-term clinical and radiographic outcomes of hallux valgus correction using the Mini TightRope.METHODS: A total of 14 cases of hallux valgus correction using the Mini TightRope technique with a 1.1-mm drill and mini-buttress plate were reviewed. Clinical examinations and radiographs were performed preoperatively and postoperatively at 1-week (non-weight-bearing), 3-month (weight-bearing), and 6-month (weight-bearing) follow-up.RESULTS: The overall 1-week postoperative decreases in IMA and hallux valgus angle (HVA) of all cases compared with preoperative status were 9 degrees and 28 degrees, respectively. Decreases in IMA and HVA continued at 3 months postoperatively but to a lesser extent, with decreases of 7 degrees and 20 degrees, respectively. Reductions in IMA and HVA were maintained through 6 months of follow-up compared with preoperatively, with IMA and HVA decreases of 6 degrees and 19 degrees, respectively. Two minor soft-tissue complications and 1 intraoperative second metatarsal fracture were treated with a buttress plate, with uneventful healing.CONCLUSIONS: Overall short-term results demonstrated notable improvements in IMA and HVA with use of the Mini TightRope, and few early complications were associated with the procedure.
|
['Adult', 'Female', 'Foot Joints', 'Hallux Valgus', 'Humans', 'Male', 'Middle Aged', 'Orthopedic Fixation Devices', 'Radiography', 'Retrospective Studies', 'Toe Joint', 'Treatment Outcome', 'Wound Healing']
| 23,559,614
|
[['M01.060.116'], ['A02.835.583.378'], ['C05.330.610'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['E07.858.442.660', 'E07.858.690.725'], ['E01.370.350.700'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['A02.835.583.378.900'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['G16.762.891']]
|
['Named Groups [M]', 'Anatomy [A]', 'Diseases [C]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Mobile marine predators: an understudied source of nutrients to coral reefs in an unfished atoll.
|
Animal movements can facilitate important ecological processes, and wide-ranging marine predators, such as sharks, potentially contribute significantly towards nutrient transfer between habitats. We applied network theory to 4 years of acoustic telemetry data for grey reef sharks (Carcharhinus amblyrhynchos) at Palmyra, an unfished atoll, to assess their potential role in nutrient dynamics throughout this remote ecosystem. We evaluated the dynamics of habitat connectivity and used network metrics to quantify shark-mediated nutrient distribution. Predator movements were consistent within year, but differed between years and by sex. Females used higher numbers of routes throughout the system, distributing nutrients over a larger proportion of the atoll. Extrapolations of tagged sharks to the population level suggest that prey consumption and subsequent egestion leads to the heterogeneous deposition of 94.5 kg d-1 of nitrogen around the atoll, with approximately 86% of this probably derived from pelagic resources. These results suggest that sharks may contribute substantially to nutrient transfer from offshore waters to near-shore reefs, subsidies that are important for coral reef health.
|
['Acoustics', 'Animal Migration', 'Animals', 'Coral Reefs', 'Ecosystem', 'Female', 'Male', 'Nitrogen Cycle', 'Nutrients', 'Population Density', 'Predatory Behavior', 'Seawater', 'Sex Factors', 'Sharks', 'Telemetry', 'Time Factors']
| 29,563,260
|
[['H01.671.031'], ['F01.145.113.069.500'], ['B01.050'], ['G16.500.275.157.130', 'N06.230.124.130'], ['G16.500.275.157', 'N06.230.124'], ['G02.111.587', 'G02.607.560', 'G16.500.768'], ['G07.203.300.681', 'J02.500.681'], ['N01.224.600', 'N06.850.505.400.600'], ['F01.145.113.111.600', 'F01.145.113.252.520'], ['G16.500.275.725.500'], ['N05.715.350.675', 'N06.850.490.875'], ['B01.050.150.900.493.370.853'], ['E01.370.520.750', 'E05.925', 'L01.178.847.675'], ['G01.910.857']]
|
['Disciplines and Occupations [H]', 'Psychiatry and Psychology [F]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Technology, Industry, and Agriculture [J]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Information Science [L]']
| 0
| 1
| 0
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
|
Changes in choroidal thickness in relation to the severity of retinopathy and macular edema in type 2 diabetic patients.
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PURPOSE: To assess changes in choroidal thickness in type 2 diabetic patients with diabetic retinopathy (DR) and diabetic macular edema (DME) using enhanced-depth imaging spectral domain optical coherence tomography (EDI-OCT).METHODS: Among 235 eyes from 145 patients, 195 treatment-na?ve eyes were divided into no DR, mild/moderate nonproliferative DR (NPDR), severe NPDR, proliferative DR (PDR), and 40 eyes having a history of laser panretinal photocoagulation (PRP) were classified as PRP-treated DR. Eyes with no ocular treatment (195 eyes) were divided according to the presence of and to the subtypes of DME. Subfoveal choroidal thickness (SFChT) and parafoveal choroidal thickness (PFChT) at 1500 ìm were measured using EDI-OCT.RESULTS: Mean age was 62.6 ± 12.4 years, and mean duration of DM was 15.1 ± 7.2 years. Mean SFChT in groups with no DR (40 eyes), mild/moderate NPDR (47 eyes), severe NPDR (72 eyes), PDR (36 eyes), and PRP-treated DR (40 eyes) was 262.3 ± 68.4 ìm, 244.6 ± 77.0 ìm, 291.1 ± 107.7 ìm, 363.5 ± 74.9 ìm, and 239.9 ± 57.4 ìm, respectively. Mean SFChT was significantly greater in eyes with PDR than in those with no DR (P < 0.01), mild/moderate NPDR (P < 0.01), or severe NPDR (P < 0.05). Mean SFChT decreased significantly in PRP-treated DR compared with PDR (P < 0.01). Eyes with DME (67 eyes) had a thicker subfoveal choroid than eyes without DME (128 eyes; P < 0.05) and, compared with cystoid or diffuse types, SFChT was thickest in subretinal detachment (SRD)-type DME (P < 0.05).CONCLUSIONS: Choroidal thickness increased significantly as the severity worsened from mild/moderate/NPDR to PDR, and decreased in PRP-treated eyes. The subfoveal choroid was thicker in eyes with DME than in those without, and was thickest in eyes with SRD-type DME.
|
['Choroid', 'Diabetes Mellitus, Type 2', 'Diabetic Retinopathy', 'Disease Progression', 'Female', 'Fluorescein Angiography', 'Humans', 'Macular Edema', 'Male', 'Middle Aged', 'Reproducibility of Results', 'Republic of Korea', 'Retina', 'Retrospective Studies', 'Severity of Illness Index', 'Tomography, Optical Coherence']
| 23,611,988
|
[['A09.371.894.223'], ['C18.452.394.750.149', 'C19.246.300'], ['C11.768.257', 'C14.907.320.382', 'C19.246.099.500.382'], ['C23.550.291.656'], ['E01.370.370.050.350', 'E01.370.380.250'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C11.768.585.439.245'], ['M01.060.116.630'], ['E05.318.370.725', 'E05.337.851', 'N05.715.360.325.685', 'N06.850.520.445.725'], ['Z01.252.474.557.750'], ['A09.371.729'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E05.318.308.980.438.475.456.500', 'N05.715.360.300.800.438.375.364.500', 'N06.850.520.308.980.438.475.364.500'], ['E01.370.350.589.249.500', 'E01.370.350.825.805.500', 'E05.642.249.500']]
|
['Anatomy [A]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Named Groups [M]', 'Health Care [N]', 'Geographicals [Z]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Management of condylomata acuminata with the carbon dioxide laser.
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Ninety-four patients with condylomata acuminata of the lower genital tract and perianal region were treated with the carbon dioxide laser. Most were managed in the outpatient clinic, but 1 to 4 treatments were required depending upon extent of involvement. Of 90 patients, 75 (83.3%) were free of lesions on all follow-up examinations after initial treatment. Of 15 patients with recurrent lesions, 13 underwent a second laser treatment; 7 of these 13 have been subsequently free of condylomata. The overall success rate was 91%. The carbon dioxide laser provides an appealing method of management of condylomata acuminata because of its precision, rapid healing without scarring, and safety when used during pregnancy.
|
['Adolescent', 'Adult', 'Anus Neoplasms', 'Carbon Dioxide', 'Condylomata Acuminata', 'Female', 'Genital Neoplasms, Female', 'Humans', 'Laser Therapy', 'Middle Aged', 'Uterine Cervical Neoplasms', 'Vaginal Neoplasms', 'Vulvar Neoplasms']
| 6,804,894
|
[['M01.060.057'], ['M01.060.116'], ['C04.588.274.476.411.307.790.040', 'C06.301.371.411.307.790.040', 'C06.405.249.411.307.790.040', 'C06.405.469.491.307.790.040', 'C06.405.469.860.101.163', 'C06.405.469.860.180.500.040'], ['D01.200.200', 'D01.362.150', 'D01.650.550.200'], ['C01.221.812.640.220', 'C01.778.640.220', 'C01.925.256.650.810.217', 'C01.925.813.220', 'C01.925.825.810.110', 'C01.925.928.914.217', 'C17.800.838.790.810.110'], ['C04.588.945.418', 'C13.351.937.418'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.594', 'E04.014.520'], ['M01.060.116.630'], ['C04.588.945.418.948.850', 'C13.351.500.852.593.131', 'C13.351.500.852.762.850', 'C13.351.937.418.875.850'], ['C04.588.945.418.955', 'C13.351.500.894.834', 'C13.351.937.418.937'], ['C04.588.945.418.968', 'C13.351.500.944.819', 'C13.351.937.418.968']]
|
['Named Groups [M]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Mucoepidermoid carcinoma of the thyroid: a tumour histotype characterised by P-cadherin neoexpression and marked abnormalities of E-cadherin/catenins complex.
|
The cadherin/catenins complex regulates cell-cell adhesion and motility and is believed to have an invasion suppressor role. Primary mucoepidermoid carcinoma of the thyroid (MECT) is a rare tumour characterised by a distinct morphological appearance and a questionable histogenesis. The coexistence of papillary thyroid carcinoma (PTC) foci in many patients with MECT suggests an association between the two tumour histotypes. In an attempt to clarify the putative relationship between MECT and PTC, we analysed tissue from 11 patients with MECT by immunohistochemistry (E-, P- and N-cadherins and alpha-, beta- and gamma-catenins). The E-cadherin gene was also analysed by polymerase chain reaction (PCR)/single-strand conformation polymorphism (SSCP). The results were compared with a control group of normal thyroid, classical PTC and the diffuse sclerosing variant of PTC. Compared with normal thyroid and PTC, MECT displays marked abnormalities of the cadherin/catenin complex. Such abnormalities include the consistent neoexpression of P-cadherin and major alterations in the expression of E-cadherin and the three catenins. Our results point to the close relationship between the de novo expression of P-cadherin and the disruption of the cadherin/catenins complex with the squamoid phenotype of MECT.
|
['Adolescent', 'Adult', 'Aged', 'Cadherins', 'Carcinoma, Mucoepidermoid', 'Child', 'Cytoskeletal Proteins', 'Desmoplakins', 'Female', 'Gene Expression', 'Humans', 'Immunohistochemistry', 'Male', 'Middle Aged', 'Polymerase Chain Reaction', 'Polymorphism, Single-Stranded Conformational', 'Thyroid Neoplasms', 'Trans-Activators', 'alpha Catenin', 'beta Catenin']
| 12,021,924
|
[['M01.060.057'], ['M01.060.116'], ['M01.060.116.100'], ['D12.776.395.550.200.200', 'D12.776.543.550.200.200', 'D23.050.301.350.200'], ['C04.557.470.200.025.340', 'C04.557.470.590.340'], ['M01.060.406'], ['D12.776.220'], ['D12.776.220.790.500'], ['G05.297'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.225.500.607.512', 'E01.370.225.750.551.512', 'E05.200.500.607.512', 'E05.200.750.551.512', 'E05.478.583', 'H01.158.100.656.234.512', 'H01.158.201.344.512', 'H01.158.201.486.512', 'H01.181.122.573.512', 'H01.181.122.605.512'], ['M01.060.116.630'], ['E05.393.620.500'], ['G05.365.795.600'], ['C04.588.322.894', 'C04.588.443.915', 'C19.344.894', 'C19.874.788'], ['D12.776.260.755', 'D12.776.930.900', 'D12.776.964.925.984'], ['D12.776.220.145.249'], ['D12.776.091.249', 'D12.776.220.145.500', 'D12.776.930.130']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 1
| 0
| 0
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The relationship of Irgarol and its major metabolite to resident phytoplankton communities in a Maryland marina, river and reference area.
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The objectives of this study were to: (1) measure water column concentrations of Irgarol 1051 and its major metabolite GS26575 annually (2004-2006) during mid-June and mid-August at 14 sites in a study area comprised of three sub-regions chosen to reflect a gradient in Irgarol exposure (Port Annapolis marina, Severn River and Severn River reference area); (2) use a probabilistic approach to determine ecological risk of Irgarol and its major metabolite in the study area by comparing the distribution of exposure data with toxicity-effects endpoints; and (3) measure both functional and structural resident phytoplankton parameters concurrently with Irgarol and metabolite concentrations to assess relationships and determine ecological risk at six selected sites in the three study areas described above. The three-year summer mean Irgarol concentrations by site clearly showed a gradient in concentrations with greater values in Back Creek (400-500ng/L range), lower values in the Severn River sites near the confluence with Back Creek (generally values less than 100ng/L) and still lower values (<10ng/L) at the Severn River reference sites at the confluence with Chesapeake Bay. A similar spatial trend, but with much lower concentrations, was also reported for GS26575. The probability of exceeding the Irgarol plant 10th centile of 193ng/L and the microcosm NOEC (323ng/L) suggested high ecological risk from Irgarol exposure at Port Annapolis marina sites but much lower risk at the other sites. There were no statistically significant differences among the three site types (marina, river and reference) with all years combined or among years within a site type for the following functional and structural phytoplankton endpoints: algal biomass, gross photosynthesis, biomass normalized photosynthesis, chlorophyll a, chlorophyll a normalized photosynthesis and taxa richness. Therefore, based on the above results, Irgarol adverse effects predicted from the plant 10th centile and the microcosm NOEC in the high Irgarol exposure area (Back Creek/Port Annapolis marina) were not confirmed with the actual field data for the receptor species (phytoplankton). These results also highlight the importance of unconfined field studies with a chemical gradient in providing valuable information regarding the responses of resident phytoplankton to herbicides.
|
['Biomass', 'Chlorophyll', 'Chlorophyll A', 'Environmental Monitoring', 'Maryland', 'Photosynthesis', 'Phytoplankton', 'Risk Assessment', 'Rivers', 'Triazines']
| 19,296,991
|
[['G16.500.275.157.100', 'N06.230.124.100'], ['D03.383.129.578.840.374', 'D03.633.400.909.374', 'D04.345.783.374'], ['D03.383.129.578.840.374.140', 'D03.633.400.909.374.140', 'D04.345.783.374.140'], ['N06.850.460.350.080', 'N06.850.780.375'], ['Z01.107.567.875.075.418', 'Z01.107.567.875.500.500'], ['G02.111.158.937', 'G02.111.669.700', 'G02.740.921', 'G03.191.937', 'G03.493.700', 'G03.800.700', 'G15.568'], ['B05.080.500.600'], ['E05.318.740.600.800.715', 'N04.452.871.715', 'N05.715.360.750.625.700.690', 'N06.850.505.715', 'N06.850.520.830.600.800.715'], ['G01.311.750', 'G16.500.275.280.650', 'N06.230.232.650'], ['D03.383.931']]
|
['Phenomena and Processes [G]', 'Health Care [N]', 'Chemicals and Drugs [D]', 'Geographicals [Z]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
|
Manganese-induced NF-kappaB activation and nitrosative stress is decreased by estrogen in juvenile mice.
|
Manganese toxicity can cause a neurodegenerative disorder affecting cortical and basal ganglia structures with a neurological presentation resembling features of Parkinson's disease. Children are more sensitive to Mn-induced neurological dysfunction than adults, and recent studies from our laboratory revealed a marked sensitivity of male juvenile mice to neuroinflammatory injury from Mn, relative to females. To determine the role of estrogen (E2) in mediating sex-dependent vulnerability to Mn-induced neurotoxicity, we exposed transgenic mice expressing an NF-êB-driven enhanced green fluorescent protein (EGFP) reporter construct (NF-êB-EGFP mice) to Mn, postulating that supplementing male mice with E2 during juvenile development would attenuate neuroinflammatory changes associated with glial activation, including expression of inducible nitric oxide synthase (NOS2) and neuronal protein nitration. Juvenile NF-êB-EGFP mice were separated in groups composed of females, males, and males surgically implanted with Silastic capsules containing 25 ìg of 17-â-estradiol (E2) or vehicle control. Mice were then treated with 0 or 100 mg/Kg MnCl(2) by intragastric gavage from postnatal days 21-34. Manganese treatment caused alterations in levels of striatal dopamine, as well as increases in NF-êB reporter activity and NOS2 expression in both microglia and astrocytes that were prevented by supplementation with E2. E2 also decreased neuronal protein nitration in Mn-treated mice and inhibited apoptosis in striatal neurons cocultured with Mn-treated astrocytes in vitro. These data indicate that E2 protects against Mn-induced neuroinflammation in developing mice and that NF-êB is an important regulator of neuroinflammatory gene expression in glia associated with nitrosative stress in the basal ganglia during Mn exposure.
|
['Animals', 'Apoptosis', 'Astrocytes', 'Cells, Cultured', 'Coculture Techniques', 'Dopamine', 'Estradiol', 'Female', 'Fluorescent Antibody Technique', 'Gene Expression Regulation', 'Genes, Reporter', 'Green Fluorescent Proteins', 'Male', 'Manganese', 'Manganese Poisoning', 'Mice', 'Mice, Transgenic', 'Microglia', 'Models, Animal', 'NF-kappa B', 'Neurodegenerative Diseases', 'Neurons', 'Nitric Oxide Synthase Type II', 'RNA, Messenger', 'Sensitivity and Specificity']
| 21,512,103
|
[['B01.050'], ['G04.146.954.035'], ['A08.637.200', 'A11.650.200'], ['A11.251'], ['E05.481.500.374'], ['D02.092.211.215.406', 'D02.092.311.342', 'D02.455.426.559.389.657.166.175.342'], ['D04.210.500.365.415.248', 'D06.472.334.851.437.500'], ['E01.370.225.500.607.512.240', 'E01.370.225.750.551.512.240', 'E05.200.500.607.512.240', 'E05.200.750.551.512.240', 'E05.478.583.375'], ['G05.308'], ['G05.360.340.024.340.435'], ['D12.776.532.265'], ['D01.268.556.484', 'D01.268.956.374', 'D01.552.544.484'], ['C10.720.475.500', 'C25.723.522.813'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.136.500', 'B01.050.150.900.649.313.992.635.505.500.800'], ['A08.637.400', 'A11.650.400'], ['E05.598'], ['D05.500.672', 'D12.776.260.600', 'D12.776.660.600', 'D12.776.930.600'], ['C10.574'], ['A08.675', 'A11.671'], ['D08.811.682.664.500.772.500', 'D12.776.157.687.575', 'D12.776.660.720.575'], ['D13.444.735.544'], ['E05.318.370.800', 'E05.318.740.872', 'G17.800', 'N05.715.360.325.700', 'N05.715.360.750.725', 'N06.850.520.445.800', 'N06.850.520.830.872']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Health Care [N]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Unravelling the mechanism of action of enzyme replacement therapy in Fabry disease.
|
Fabry disease (FD) is a rare X-linked recessive glycosphingolipid-storage disorder caused by deficient activity of the lysosomal enzyme alpha-galactosidase A. Intravenous enzyme replacement therapy (ERT) has been used to supplement deficient enzyme activity in patients with FD. Despite its clinical effect and manifestations, clear criteria for the clinical effectiveness and cost-effectiveness of ERT have not been well established. In this study, we investigated the pharmacodynamic actions and short-term effects of ERT in patients with FD through direct molecular profiling from blood samples of patients before and after ERT. Based on this comparison, we observed that immune/inflammation-related pathways and growth factor-related pathways such as innate/adaptive immune pathway, lymphocyte proliferation and leukocyte proliferation were actively regulated under ERT. We also found that TINAGL1, DAAM2, CDK5R1 and MYO5B known to be related with clinical symptoms of FD showed increased levels after ERT, leading to the amelioration of clinical manifestations. Especially the catabolic process-related genes, including USP15 and ERUN1, showed direct increasing after ERT in vivo in male patients. These results suggest that male patients with FD respond more actively to ERT than do female patients with FD. Pathway analysis revealed that oxidative phosphorylation pathway-related genes are downregulated under ERT. ERT has a role to protect the proteins from oxidative damage and such deactivation of oxidative phosphorylation is one of direct pharmacodynamic actions of ERT. These results extended our understanding of the pathophysiology of ERT. To our knowledge, this is the first study to observe the molecular basis for the mechanism of ERT in vivo through the comprehensive comparison of transcriptome study with next-generation sequencing data.
|
['Adolescent', 'Adult', 'Enzyme Replacement Therapy', 'Fabry Disease', 'Female', 'Gene Expression Regulation', 'Gene Regulatory Networks', 'Humans', 'Male', 'Sex Factors', 'Transcriptome', 'Young Adult']
| 26,490,183
|
[['M01.060.057'], ['M01.060.116'], ['E02.319.353.500'], ['C10.228.140.163.100.435.825.200', 'C10.228.140.300.275.374', 'C14.907.253.329.374', 'C16.320.322.124', 'C16.320.565.189.435.825.200', 'C16.320.565.398.641.803.300', 'C16.320.565.595.554.825.200', 'C18.452.132.100.435.825.200', 'C18.452.584.687.803.300', 'C18.452.648.189.435.825.200', 'C18.452.648.398.641.803.300', 'C18.452.648.595.554.825.200'], ['G05.308'], ['G05.360.080.689.360'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['N05.715.350.675', 'N06.850.490.875'], ['G02.111.873.750', 'G05.297.700.750', 'G05.360.920'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Health Care [N]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Cyclic hair-loss and regrowth in transgenic mice overexpressing an intermediate filament gene.
|
We have produced transgenic mice containing up to 250 copies of a sheep wool intermediate filament keratin gene to study the effect of its expression on hair structure and development. Several transgenic lines expressed the gene and in the one containing 250 transgenes, a pattern of hair-loss and regrowth was stably established. Successive waves of hair growth follow periods of denuding like the natural progression of hairs in the mouse hair cycle. By in situ hybridization we have shown that the sheep transgenes are expressed at the correct stage in mouse hair development and at a high level. The transgenic hairs contain not only an elevated level of intermediate filament keratin protein but also a decreased level of the filament-associated proteins. This imbalance disrupts the normal ordered array of these proteins in the cells of the hair cortex and leads to weakened fibres which are prematurely lost.
|
['Amino Acids', 'Animals', 'Hair', 'Intermediate Filament Proteins', 'Keratins', 'Mice', 'Mice, Transgenic', 'Microscopy, Electron', 'Mutation', 'Nucleic Acid Hybridization', 'RNA Probes', 'Restriction Mapping', 'Sheep', 'Wool']
| 1,691,707
|
[['D12.125'], ['B01.050'], ['A17.360'], ['D05.750.078.593', 'D12.776.220.475'], ['D05.750.078.593.450', 'D12.776.220.475.450', 'D12.776.860.607'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.136.500', 'B01.050.150.900.649.313.992.635.505.500.800'], ['E01.370.350.515.402', 'E05.595.402'], ['G05.365.590'], ['E05.393.661', 'G02.111.611'], ['D13.444.600.723', 'D27.505.259.750.600.825', 'D27.720.470.530.600.825'], ['E05.393.183.620.650', 'E05.393.712'], ['B01.050.150.900.649.313.500.380.791'], ['A13.970', 'A17.360.855']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[Two cases of Lactobacillus rhamnosus infection and pancreatitis].
|
Lactobacilli rhamnosus infections are rare and occur in patients with severe conditions causing immunosuppression and/or in those who have received intense antibiotic treatment such as with teicoplanin and vancomycin. We report two cases which occurred within a period of 48 hours, during regression of pancreatitis in two women aged 35 and 70 years old, hospitalized in the same department. Several criteria pointed towards two independent infections but the rarity and chronology of the appearance of these infections raised the question of possible cross-contamination. Lactobacillus rhamnosus was isolated in intra-abdominal samples from both patients, and in blood cultures from one of them. Specimens were monomicrobial. The description of these observations shows the limits of molecular biology for identifying the Lactobacillus rhamnosus strain: it was not possible to determine whether a single clone or two different clones were involved.
|
['Adult', 'Aged', 'Antidepressive Agents', 'C-Reactive Protein', 'Depression', 'Female', 'Gram-Positive Bacterial Infections', 'Humans', 'Lactobacillus rhamnosus', 'Pancreatitis']
| 18,096,340
|
[['M01.060.116'], ['M01.060.116.100'], ['D27.505.954.427.700.122'], ['D12.776.034.145', 'D12.776.124.050.120', 'D12.776.124.486.157'], ['F01.145.126.350'], ['C01.150.252.410'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['B03.353.750.450.475.700', 'B03.510.460.400.410.475.475.700', 'B03.510.550.450.475.700'], ['C06.689.750']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Psychiatry and Psychology [F]', 'Diseases [C]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Living with incomprehensible fatigue after recent myocardial infarction.
|
AIM: This paper is a report of a study of what fatigue means to patients with recent myocardial infarction (MI) and how they manage to deal with the consequences of this symptom.BACKGROUND: After MI, fatigue is a frequent and distressing symptom. In nursing practice and in everyday conversations, the term 'tiredness' has a broad meaning that is often used synonymously with fatigue. Fatigue may be defined as a state along an adaptation continuum with tiredness and exhaustion as distinct states at the ends of the continuum.METHOD: In accordance with a constructivist grounded theory method, 19 patients were interviewed four months after having a MI. The informants were chosen from a larger sample of patients admitted to a coronary care unit during the period October 2005 to September 2006.FINDINGS: Living with incomprehensible fatigue was identified as the central theme, which described what fatigue meant to patients 4 months after their MI and how they handled it. The core category was labelled incomprehensible fatigue. Two of the categories refer to consequences: being restricted and feeling defeated and one category describes management: fumbling coping strategies. Finally, one category concerns the outcome: moderate relief of fatigue.CONCLUSION: Nursing interventions could focus on identifying and reducing stressors as well as on increasing patients' ability to cope with stressors. Further research should focus on identifying stressors and useful coping strategies after MI, knowledge that could be used to prevent aggravation of fatigue.
|
['Adaptation, Psychological', 'Adult', 'Aged', 'Fatigue', 'Female', 'Hospitals, Rural', 'Humans', 'Male', 'Middle Aged', 'Myocardial Infarction', 'Quality of Life', 'Severity of Illness Index', 'Stress, Psychological', 'Surveys and Questionnaires', 'Time Factors']
| 19,146,515
|
[['F01.058'], ['M01.060.116'], ['M01.060.116.100'], ['C23.888.369'], ['N02.278.421.518'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['C14.280.647.500', 'C14.907.585.500', 'C23.550.513.355.750', 'C23.550.717.489.750'], ['I01.800', 'K01.752.400.750', 'N06.850.505.400.425.837'], ['E05.318.308.980.438.475.456.500', 'N05.715.360.300.800.438.375.364.500', 'N06.850.520.308.980.438.475.364.500'], ['F01.145.126.990', 'F02.830.900'], ['E05.318.308.980', 'N05.715.360.300.800', 'N06.850.520.308.980'], ['G01.910.857']]
|
['Psychiatry and Psychology [F]', 'Named Groups [M]', 'Diseases [C]', 'Health Care [N]', 'Organisms [B]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Humanities [K]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 0
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 0
|
Neuro-oncology perspective of treatment options in metastatic breast cancer.
|
Breast cancer (BC) is a heterogeneous disease. Different subtypes of BC exhibit a peculiar natural history, metastatic potential and outcome. Stereotactic radiosurgery is the most used treatment for brain metastases (BM), while surgery is reserved for large and symptomatic lesions. Whole-brain radiotherapy is employed in multiple BM not amendable to radiosurgery or surgery, and it is not employed any more following local treatments of a limited number of BM. A critical issue is the distinction from pseudoprogression or radionecrosis, and tumor regrowth. Considering the increase of long-term survivors after combined or novel treatments for BM, cognitive dysfunctions following whole-brain radiotherapy represent an issue of utmost importance. Neuroprotective drugs and innovative radiotherapy techniques are being investigated to reduce this risk of cognitive sequelae. Leptomeningeal disease represents a devastating complication, either alone or in association to BM, thus targeted therapies are employed in HER2-positive BC brain and leptomeningeal metastases.
|
['Brain Neoplasms', 'Breast Neoplasms', 'Cranial Irradiation', 'Female', 'Humans', 'Neuroprotective Agents', 'Prognosis', 'Quality of Life', 'Radiosurgery']
| 29,956,562
|
[['C04.588.614.250.195', 'C10.228.140.211', 'C10.551.240.250'], ['C04.588.180', 'C17.800.090.500'], ['E02.815.190'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D27.505.696.706.548', 'D27.505.954.427.575'], ['E01.789'], ['I01.800', 'K01.752.400.750', 'N06.850.505.400.425.837'], ['E02.815.530', 'E04.525.800.650', 'E05.873.500']]
|
['Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Humanities [K]', 'Health Care [N]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 0
|
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