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Folate and vitamin B12 status of the elderly.
|
OBJECTIVE: The present study was undertaken to investigate the relationship between estimated folate and vitamin B12 intakes and their biochemical status in elderly persons.SUBJECTS: Twenty-eight males and 30 females ( > 65 years) were randomly selected from a larger sample of free-living elderly residents of Edmonton, Canada. Subjects were contacted through a seniors' service organization. Any subject using vitamin supplements or alcohol other than the social drink was excluded from the study.DESIGN: Dietary intake was estimated using a three-nonconsecutive-day food record. Biochemical status was assessed by measuring the plasma levels of folate and vitamin B12, as well as the red blood cell (r.b.c.) folate levels.RESULTS: Average daily intakes of both folate and vitamin B12 met the recommended requirements. Their mean plasma levels were within the accepted normal ranges in both males and females. However, probability analysis of dietary intake revealed an appreciable number of subjects at risk of deficiency, especially of folate (male 26%; female 21%). According to the interpretive guidelines of r.b.c. folate levels, an appreciable number of the study subjects were also found to be at risk of folate deficiency (male 57%; female 67%).CONCLUSIONS: The folate status appears to be a greater concern than the vitamin B12 status for the elderly population. It seems that although mean values of dietary intake and plasma concentrations of folate may indicate nutritional adequacy, a proportion of the older population may still be at nutritional risks.
|
['Aged', 'Aging', 'Female', 'Folic Acid', 'Humans', 'Male', 'Nutritional Requirements', 'Nutritional Status', 'Reference Values', 'Risk Factors', 'Vitamin B 12']
| 8,793,412
|
[['M01.060.116.100'], ['G07.345.124'], ['D03.633.100.733.631.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G07.203.650.620'], ['G07.203.650.650', 'N01.224.425.525'], ['E05.978.810'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['D03.383.129.578.840.437.777', 'D03.633.400.909.437.777', 'D04.345.783.437.777']]
|
['Named Groups [M]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Health Care [N]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
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Analysis of clonal evolution in a tumor consisting of pSV2neo-transfected mouse fibrosarcoma clones.
|
The process of clonal evolution was analyzed in a line of methylcholanthrene-induced mouse fibrosarcomas. The tumor cells were transfected with pSV2neo gene and 22 clones were randomly isolated. Genetically tagged clones were mixed and inoculated into syngeneic mice. Southern blot analysis revealed that one of the clones, no. 11, dominated both in tumors in situ and in lung metastatic nodules. No. 11 clone and other clones were similar in growth rates in vitro and in vivo, in spontaneous and experimental metastatic abilities, in immunogenicity, and in the capacity of intercellular communication in vitro. Although no. 11 clone overgrew other clones in vivo, this was not the case when clones were mixed and maintained in vitro. We conclude that clonal interactions in vivo may be responsible for the dominance of no. 11 clone in the tumor. It is likely that the preferential metastasis of no. 11 clone to the lung may be a simple reflection of the proliferative advantage of the dominant clone in the tumor in situ.
|
['Animals', 'Cell Communication', 'Clone Cells', 'Fibrosarcoma', 'Mice', 'Mice, Inbred Strains', 'Neomycin', 'Neoplasm Metastasis', 'Transfection', 'Tumor Stem Cell Assay']
| 2,110,131
|
[['B01.050'], ['G04.085'], ['A11.251.353'], ['C04.557.450.565.590.350', 'C04.557.450.795.350'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520', 'B01.050.150.900.649.313.992.635.505.500.400'], ['D09.408.051.623'], ['C04.697.650', 'C23.550.727.650'], ['E05.393.350.810', 'G05.728.860'], ['E01.370.225.500.383.910', 'E01.370.225.500.388.930', 'E05.200.500.383.910', 'E05.200.500.388.930', 'E05.242.383.910', 'E05.242.417.500', 'E05.337.550.200.800']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
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|
Development of a biotin-streptavidin-enhanced enzyme-linked immunosorbent assay which uses monoclonal antibodies for detection of group C rotaviruses.
|
A biotin-streptavidin-enhanced enzyme-linked immunosorbent assay (ELISA) which uses monoclonal antibodies (MAbs) for the detection of group C rotaviruses was developed. An assay in which plates were coated with three pooled MAbs and biotinylated polyclonal immunoglobulin G (IgG) (polyclonal antibody [PAb]) was used as the detector (MAb capture-PAb detector) was found to be the most sensitive and specific of the assays when it was compared with assays in which plates were coated with polyclonal antiserum and detection was done with either biotinylated polyclonal antiserum (PAb capture-PAb detector) or biotinylated pooled MAbs (PAb capture-MAb detector). The MAb capture-PAb detector ELISA detected 83% of samples confirmed to be positive for group C rotaviruses, whereas the PAb capture-PAb detector assay detected 63% of positive samples and the PAb capture-MAb detector assay detected 65% of positive samples. All three procedures detected both of the bovine and the two human group C rotaviruses, but none of the three procedures detected fecal samples containing group A and B rotaviruses or fecal samples negative for group C rotaviruses used in this study. The sensitivity of the MAb capture-PAb detector ELISA was determined by serially diluting fecal group C rotaviruses; antigens were detected in maximal positive dilution ranges of 1:1,000 to 1:3,000 for the samples tested. On the basis of the cell culture immunofluorescence assay infectivity titer of semipurified cell culture-passaged Cowden group C rotavirus, the sensitivity of the MAb capture-PAb detection ELISA for detection of homologous group C rotavirus was 53 fluorescent focus units per ml. Epitope mapping by use of the biotinylated MAbs in competition assay suggested that our MAbs may bind to three different but overlapping epitopes. These results suggest that the MAb capture-PAb detector ELISA can be used to study the epidemiology of group C rotaviruses in humans and animals.
|
['Animals', 'Antibodies, Monoclonal', 'Bacterial Proteins', 'Binding, Competitive', 'Biotin', 'Cattle', 'Child', 'Enzyme-Linked Immunosorbent Assay', 'Feces', 'Humans', 'Rotavirus', 'Sensitivity and Specificity', 'Streptavidin', 'Swine']
| 1,321,166
|
[['B01.050'], ['D12.776.124.486.485.114.224', 'D12.776.124.790.651.114.224', 'D12.776.377.715.548.114.224'], ['D12.776.097'], ['E05.196.080', 'G02.111.084', 'G02.111.570.120.309'], ['D03.383.129.308.080', 'D08.211.096'], ['B01.050.150.900.649.313.500.380.271'], ['M01.060.406'], ['E05.478.566.350.170', 'E05.478.566.380.360', 'E05.478.583.400.170', 'E05.601.470.350.170', 'E05.601.470.380.360'], ['A12.459'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['B04.820.223.719.790'], ['E05.318.370.800', 'E05.318.740.872', 'G17.800', 'N05.715.360.325.700', 'N05.715.360.750.725', 'N06.850.520.445.800', 'N06.850.520.830.872'], ['D12.776.097.835'], ['B01.050.150.900.649.313.500.880']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Named Groups [M]', 'Anatomy [A]', 'Health Care [N]']
| 1
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|
Essential metal depletion in an anaerobic reactor.
|
The effect of the absence of trace elements on the conversion of a mixture of volatile fatty acids by a distillery anaerobic granular sludge was investigated. Two UASB reactors were operated under identical operational conditions except for the influent trace metal concentrations, during 140 days. Experiments were carried out in three periods, where different organic loading rates (OLR) were applied to the reactors. The total trace metal concentration steadily decreased at a rate of 48 microg metal/g TS.d in the deprived reactor (down to 35% of their initial value). In contrast, trace metals accumulated in granules present in the control reactor. At the end of the experiment, the COD removal efficiencies were 99% and 77% for the control and deprived reactors, respectively, due to the lack of propionate conversion. Cobalt sorption experiments were carried out in order to study its speciation, and its effects on the speciation of other metals as well. A paper mill wastewater treating granular sludge was also included in the study as a comparison. Results obtained showed that the principal metal forms normally associated with any sludge are a function of each soluble metal concentration in the system, and the characteristics of the particular sludge.
|
['Bacteria, Anaerobic', 'Bioreactors', 'Fatty Acids, Volatile', 'Metals', 'Sewage', 'Solubility', 'Trace Elements', 'Waste Disposal, Fluid']
| 14,640,193
|
[['B03.130'], ['E07.115', 'J01.897.120.115'], ['D10.251.400'], ['D01.552'], ['D20.944.932.500'], ['G02.805'], ['D01.268.811', 'D27.505.696.494.555', 'G07.203.300.681.500.555', 'J02.500.681.500.555'], ['N06.850.780.200.800.800.890', 'N06.850.860.510.900.600.900']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Technology, Industry, and Agriculture [J]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Health Care [N]']
| 0
| 1
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|
Venous thromboembolism and bleeding after total knee and hip arthroplasty. Findings from the Spanish National Discharge Database.
|
The impact of venous thromboembolism (VTE) and bleeding in patients undergoing major joint surgery has not been thoroughly studied. The Spanish National Discharge Database during the years 2005-2006 was used to assess the frequency and clinical impact of VTE and bleeding after elective total knee (TKA) or hip (THA) arthroplasty. Of 58,037 patients undergoing TKA, 0.18% (95% confidence interval [CI]: 0.15-0.22) were diagnosed with pulmonary embolism (PE), 0.57% (95% CI: 0.51-0.63) with deep-vein thrombosis (DVT), 1.20% (95% CI: 1.12-1.30) had bleeding complications, and 0.09% (95% CI: 0.07-0.12) died. Of 54 patients who died, 20.4% (95% CI: 10.7-35.4) had been diagnosed with PE, 3.70% (95% CI: 0.63-11.7) with DVT, and 13.0% (95% CI: 5.67-25.6) had bled. Of 31,769 patients undergoing elective THA, 0.23% (95% CI: 0.18-0.29) were diagnosed with PE, 0.44% (95% CI: 0.37-0.52) with DVT, 1.21% (95% CI: 1.10-1.34) bled, and 0.16% (95% CI: 0.12-0.21) died. Of 52 patients who died, 13.5% (95% CI: 6.08-24.8) had been diagnosed with PE, and 9.61% (95% CI: 3.52-21.3) had bled. On multivariable analysis, PE (odds ratio [OR]: 157; 95% CI: 75-328), DVT (OR: 6.3; 95% CI: 1.5-27) and bleeding (OR: 8.5; 95% CI: 3.6-20) were independent predictors for death after TKA. After THA, only PE (OR: 65; 95% CI: 26-160) and bleeding (OR: 6.4; 95% CI: 2.3-17) predicted the risk for death. Bleeding, DVT, and PE, arising after TKA were all independent predictors for death. Their increase in risk was, however, substantially higher for PE. After THA, only PE and bleeding independently predicted death.
|
['Aged', 'Aged, 80 and over', 'Arthroplasty, Replacement, Hip', 'Arthroplasty, Replacement, Knee', 'Databases, Factual', 'Female', 'Humans', 'Joint Diseases', 'Male', 'Middle Aged', 'Postoperative Hemorrhage', 'Risk', 'Spain', 'Survival Analysis', 'Venous Thromboembolism']
| 21,174,008
|
[['M01.060.116.100'], ['M01.060.116.100.080'], ['E04.555.110.110.110', 'E04.650.110.110', 'E04.680.101.110.110'], ['E04.555.110.110.115', 'E04.650.110.115', 'E04.680.101.110.115'], ['L01.313.500.750.300.188.400', 'L01.470.750.750'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C05.550'], ['M01.060.116.630'], ['C23.550.414.941', 'C23.550.767.850'], ['E05.318.740.600.800', 'G17.680.750', 'N05.715.360.750.625.700', 'N06.850.520.830.600.800'], ['Z01.542.846'], ['E05.318.740.998', 'N05.715.360.750.795', 'N06.850.520.830.998'], ['C14.907.355.590.700']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Information Science [L]', 'Organisms [B]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Geographicals [Z]']
| 0
| 1
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Time course of air hunger mirrors the biphasic ventilatory response to hypoxia.
|
Determining response dynamics of hypoxic air hunger may provide information of use in clinical practice and will improve understanding of basic dyspnea mechanisms. It is hypothesized that air hunger arises from projection of reflex brain stem ventilatory drive ("corollary discharge") to forebrain centers. If perceptual response dynamics are unmodified by events between brain stem and cortical awareness, this hypothesis predicts that air hunger will exactly track ventilatory response. Thus, during sustained hypoxia, initial increase in air hunger would be followed by a progressive decline reflecting biphasic reflex ventilatory drive. To test this prediction, we applied a sharp-onset 20-min step of normocapnic hypoxia and compared dynamic response characteristics of air hunger with that of ventilation in 10 healthy subjects. Air hunger was measured during mechanical ventilation (minute ventilation = 9 +/- 1.4 l/min; end-tidal Pco(2) = 37 +/- 2 Torr; end-tidal Po(2) = 45 +/- 7 Torr); ventilatory response was measured during separate free-breathing trials in the same subjects. Discomfort caused by "urge to breathe" was rated every 30 s on a visual analog scale. Both ventilatory and air hunger responses were modeled as delayed double exponentials corresponding to a simple linear first-order response but with a separate first-order adaptation. These models provided adequate fits to both ventilatory and air hunger data (r(2) = 0.88 and 0.66). Mean time constant and time-to-peak response for the average perceptual response (0.36 min(-1) and 3.3 min, respectively) closely matched corresponding values for the average ventilatory response (0.39 min(-1) and 3.1 min). Air hunger response to sustained hypoxia tracked ventilatory drive with a delay of approximately 30 s. Our data provide further support for the corollary discharge hypothesis for air hunger.
|
['Adult', 'Carbon Dioxide', 'Dyspnea', 'Female', 'Humans', 'Hypoxia', 'Male', 'Middle Aged', 'Pain Measurement', 'Perception', 'Reaction Time', 'Respiratory Center', 'Respiratory Mechanics']
| 15,286,044
|
[['M01.060.116'], ['D01.200.200', 'D01.362.150', 'D01.650.550.200'], ['C08.618.326', 'C23.888.852.371'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C23.888.852.079'], ['M01.060.116.630'], ['E01.370.600.550.324'], ['F02.463.593'], ['E05.796.817', 'F02.830.650', 'F04.669.817', 'G11.561.677'], ['A08.186.211.132.772.646'], ['G09.772.705.700']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
The adult hip: an anatomic study. Part II: the soft-tissue landmarks.
|
A comprehensive anatomic, pathologic, and radiographic study was undertaken to define reliable soft-tissue landmarks about the hip. Methodology included an analysis of 300 "normal" adult hip radiographs, tissue maceration, cadaveric intra-articular injection studies and review of selected clinical cases. Results, when applied to clinical situations, indicate: (a) The iliopsoas and "capsular" fat planes are poor indicators of small to moderate amounts of intra-articular fluid in the adult. (b) The "capsular" fat plane is not associated with the joint capsule, but in fact is a fat plane between two muscle bundles anterior to the articulation. (c) Distinct soft-tissue planes are available for dissemination of fluid from the hip joint. These include the iliopsoas bursa, which may distend in association with articular disease, and the fat plane of the obturator externus muscle.
|
['Hip', 'Hip Joint', 'Humans', 'Radiography']
| 663,195
|
[['A01.378.610.400'], ['A02.835.583.411'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.350.700']]
|
['Anatomy [A]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
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Effects of chondroitin sulfate on trabecular meshwork in rabbit eyes: an electron microscopic study.
|
The intraocular pressure of four New Zealand albino rabbit eyes was elevated when we replaced the aqueous humor of these eyes repeatedly with a chondroitin sulfate solution. Seen by electron microscopy, the trabecular meshwork of these eyes showed moderately increased collagen fibers, elastic fibers, and fine fibrils, and thickening of basement membrane. The amount of extracellular material present was markedly increased when compared with control eyes. Three types of basement membranes were noted. Compact multilaminated basement membrane and placoid accumulations of filamentous material with a granular background were found adjacent to the endothelial cells of the trabecular meshwork, and fine fibrils were observed around the angular aqueous veins. The ultrastructural alterations we observed were similar to those seen in some human glaucoma cases. The metabolism of trabecular meshwork cells may be affected by the long-term chondroitin sulfate treatment. As a result, extracellular matrix material appears to be accumulated in the trabecular meshwork, which may then contribute to an increased outflow resistance and a mild intraocular pressure elevation.
|
['Animals', 'Chondroitin', 'Chondroitin Sulfates', 'Intraocular Pressure', 'Microscopy, Electron', 'Rabbits', 'Trabecular Meshwork']
| 6,440,803
|
[['B01.050'], ['D09.698.373.200'], ['D09.698.373.200.300'], ['G14.440'], ['E01.370.350.515.402', 'E05.595.402'], ['B01.050.150.900.649.313.968.700'], ['A09.371.060.932']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
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| 0
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|
Reversal of reproductive deficiency in the hpg male mouse by neonatal androgenization.
|
Some aspects of reproductive function in the GnRH-deficient hypogonadal (hpg) mutant mouse can be restored by transplanting normal fetal brain tissue containing GnRH cells into the central nervous system of adult hpg mice. However, hpg males showing physiological response to the graft fail to display sexual behavior and are infertile. We hypothesized that the reproductive deficit of these males is due to insufficient perinatal exposure to testicular androgens as a consequence of the GnRH deficiency. To test this hypothesis we androgenized hpg males by giving them neonatal injections of testosterone propionate (TP). Controls consisted of hpg males not androgenized neonatally and of normal males. All three groups received a TP implant in adulthood, and their copulatory behavior and reproductive capability were recorded. In addition, other hpg males, not androgenized neonatally, received fetal brain transplants containing GnRH neurons and were also tested for copulatory behavior and reproductive capability before and after receiving a TP implant. Three of 8 neonatally androgenized hpg males expressed the full repertoire of male sexual behavior, including intromission and ejaculation, and sired several litters. Three of 7 control hpg males that were not androgenized neonatally but received TP implants in adulthood also displayed mounting and intromission, but there was no evidence of ejaculation, and these males failed to impregnate normal females. Of the 8 hpg males that responded to a fetal transplant with testicular growth, only 1 displayed mounting behavior. However, when given a TP implant, 4 of 8 hpg males with grafts displayed mounting and intromissions.(ABSTRACT TRUNCATED AT 250 WORDS)
|
['Androgens', 'Animals', 'Animals, Newborn', 'Brain Tissue Transplantation', 'Fetal Tissue Transplantation', 'Follicle Stimulating Hormone', 'Gonadotropin-Releasing Hormone', 'Hypogonadism', 'Luteinizing Hormone', 'Male', 'Mice', 'Mice, Mutant Strains', 'Reproduction', 'Sexual Behavior, Animal', 'Spermatogenesis', 'Testosterone']
| 1,391,342
|
[['D27.505.696.399.472.161'], ['B01.050'], ['B01.050.050.282'], ['E02.095.147.725.090', 'E04.525.090', 'E04.936.580.090'], ['E02.095.147.725.300', 'E04.936.580.300'], ['D06.472.699.322.576.288', 'D06.472.699.631.525.343.288', 'D12.644.548.691.525.343.288'], ['D06.472.699.327.740.320', 'D12.644.400.400.740.320', 'D12.644.456.460', 'D12.644.548.365.740.320', 'D12.776.631.650.405.740.320'], ['C19.391.482'], ['D06.472.699.322.576.463', 'D06.472.699.631.525.343.463', 'D12.644.548.691.525.343.463'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.150.900.649.313.992.635.505.500.550'], ['G08.686.784'], ['F01.145.113.252.748'], ['G04.152.650.624', 'G08.686.784.310.760'], ['D04.210.500.054.079.429.824', 'D06.472.334.851.968.984']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Psychiatry and Psychology [F]']
| 0
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Complex wavefront-guided retreatments with the Alcon CustomCornea platform after prior LASIK.
|
PURPOSE: To report the results of complex wavefront-guided LASIK retreatments.METHODS: Twenty eyes (15 patients) with histories of conventional LASIK surgery and significant visual complaints of glare and halos due to higher order aberrations were treated. Wavefront-guided retreatments were performed with the LADARVision CustomCornea system (Alcon, Ft Worth, Tex). Pre- and postoperative topographies, wavefront measurements, and subjective reports were analyzed.RESULTS: Postoperatively, patients had an expanded optical zone, many with improved centration. Lower and higher order aberrations decreased following wavefront-guided ablation. Mean higher order root-mean-square decreased from 1.01 +/- 0.25 microm preoperatively to 0.84 +/- 0.23 microm postoperatively. Mean coma decreased from 0.59 +/- 0.26 microm to 0.43 +/- 0.21 microm. Mean spherical aberration decreased from 0.66 +/- 0.25 microm to 0.54 +/- 0.27 microm. Subjective reports of glare and halo symptoms improved in all patients.CONCLUSIONS: CustomCornea wavefront-guided treatments are effective in reducing lower and higher order aberrations, expanding optical zones, and improving subjective reports of adverse aberration sequelae such as glare and halos.
|
['Adult', 'Astigmatism', 'Corneal Topography', 'Equipment Design', 'Female', 'Follow-Up Studies', 'Humans', 'Keratomileusis, Laser In Situ', 'Laser Coagulation', 'Male', 'Middle Aged', 'Myopia', 'Refraction, Ocular', 'Reoperation', 'Retrospective Studies', 'Treatment Outcome', 'Visual Acuity']
| 16,447,936
|
[['M01.060.116'], ['C11.744.212'], ['E01.370.380.150'], ['E05.320'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.594.480.750', 'E04.014.520.480.750', 'E04.378.500.750', 'E04.540.825.437.374'], ['E02.520.745.410', 'E02.594.530', 'E04.014.520.530', 'E04.350.750.410', 'E04.540.630.410'], ['M01.060.116.630'], ['C11.744.636'], ['E01.370.380.850.700', 'G01.590.775', 'G14.760'], ['E04.690'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['E01.370.380.850.950', 'F02.463.593.932.901', 'G14.940']]
|
['Named Groups [M]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Psychiatry and Psychology [F]']
| 0
| 1
| 1
| 0
| 1
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| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
[Tetraplegia, resuscitation and epileptic seizures after partial colon resection in a 41-year-old woman].
|
A 41-year-old woman, suffering from continuous abdominal pain, only presented a non-specific inflammation of the whole colon and an unclaryfied hyponatriaemia; in spite of the only doubtful explanation by an enormous elongation of the colon, it was partially resected. Thereafter, the patient's decline, hypaesthesia, areflexia and tetraparesis required intensive care. Despite immunoglobulin therapy, assuming a Guillain-Barr? syndrome, the patient needed resuscitation, followed by signs of severe hypoxia (high level of neuron-specific enolase, hippocampal lesions). The abdominal pain, hyponatriaemia, persistent tachycardia, sensory deficits, tetraplegia, circulation arrest, later epileptic seizures and unusual urine color were finally explained by an acute intermittent porphyria (AIP). Although the symptoms were classic, the disease was recognized only very late. Indeed, it is so rare that most physicians will never be confronted with an AIP or only once or twice.
|
['Acute Disease', 'Adult', 'Colectomy', 'Diagnosis, Differential', 'Epilepsy', 'Female', 'Humans', 'Porphyria, Acute Intermittent', 'Quadriplegia', 'Resuscitation', 'Treatment Outcome']
| 24,399,476
|
[['C23.550.291.125'], ['M01.060.116'], ['E04.210.219'], ['E01.171'], ['C10.228.140.490'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C06.552.830.150', 'C16.320.850.742.150', 'C17.800.827.742.150', 'C18.452.811.400.150'], ['C10.597.622.760', 'C23.888.592.636.786'], ['E02.365.647'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Diseases [C]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Health Care [N]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
ERK1/2 pathway is involved in renal gluconeogenesis inhibition under conditions of lowered NADPH oxidase activity.
|
The aim of this study was to elucidate the mechanisms involved in the inhibition of renal gluconeogenesis occurring under conditions of lowered activity of NADPH oxidase (Nox), the enzyme considered to be one of the main sources of reactive oxygen species in kidneys. The in vitro experiments were performed on primary cultures of rat renal proximal tubules, with the use of apocynin, a selective Nox inhibitor, and TEMPOL (4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl), a potent superoxide radical scavenger. In the in vivo experiments, Zucker diabetic fatty (ZDF) rats, a well established model of diabetes type 2, were treated with apocynin solution in drinking water. The main in vitro findings are the following: (1) both apocynin and TEMPOL attenuate the rate of gluconeogenesis, inhibiting the step catalyzed by phosphoenolpyruvate carboxykinase (PEPCK), a key enzyme of the process; (2) in the presence of the above-noted compounds the expression of PEPCK and the phosphorylation of transcription factor CREB and ERK1/2 kinases are lowered; (3) both U0126 (MEK inhibitor) and 3-(2-aminoethyl)-5-((4-ethoxyphenyl)methylene)-2,4-thiazolidinedione (ERK inhibitor) diminish the rate of glucose synthesis via mechanisms similar to those of apocynin and TEMPOL. The observed apocynin in vivo effects include: (1) slight attenuation of hyperglycemia; (2) inhibition of renal gluconeogenesis; (3) a decrease in renal PEPCK activity and content. In view of the results summarized above, it can be concluded that: (1) the lowered activity of the ERK1/2 pathway is of importance for the inhibition of renal gluconeogenesis found under conditions of lowered superoxide radical production by Nox; (2) the mechanism of this phenomenon includes decreased PEPCK expression, resulting from diminished activity of transcription factor CREB; (3) apocynin-evoked inhibition of renal gluconeogenesis contributes to the hypoglycemic action of this compound observed in diabetic animals. Thus, the study has delivered some new insights into the recently discussed issue of the usefulness of Nox inhibition as a potential antidiabetic strategy.
|
['Acetophenones', 'Animals', 'Antioxidants', 'Butadienes', 'Cyclic AMP Response Element-Binding Protein', 'Cyclic N-Oxides', 'Diabetes Mellitus, Experimental', 'Gene Expression Regulation', 'Gluconeogenesis', 'Hypoglycemic Agents', 'Intracellular Signaling Peptides and Proteins', 'Kidney Tubules, Proximal', 'Male', 'Mitogen-Activated Protein Kinase 1', 'Mitogen-Activated Protein Kinase 3', 'NADPH Oxidases', 'Nitriles', 'Phosphoenolpyruvate Carboxykinase (GTP)', 'Primary Cell Culture', 'Protein Kinase Inhibitors', 'Rats', 'Rats, Zucker', 'Reactive Oxygen Species', 'Signal Transduction', 'Spin Labels', 'Thiazolidinediones']
| 25,601,753
|
[['D02.522.120'], ['B01.050'], ['D27.505.519.217', 'D27.505.696.706.125', 'D27.720.799.047'], ['D02.455.326.271.665.146.240'], ['D12.776.260.108.184', 'D12.776.930.127.184'], ['D03.661.243'], ['C18.452.394.750.074', 'C19.246.240', 'E05.598.500.374'], ['G05.308'], ['G02.111.158.500', 'G03.191.500'], ['D27.505.696.422'], ['D12.644.360', 'D12.776.476'], ['A05.810.453.736.560.570'], ['D08.811.913.696.620.682.700.567.249.500', 'D12.644.360.450.169.500', 'D12.776.476.450.169.500'], ['D08.811.913.696.620.682.700.567.249.750', 'D12.644.360.450.169.750', 'D12.776.476.450.169.750'], ['D08.811.682.608.575', 'D12.776.331.894', 'D12.776.543.653'], ['D02.626'], ['D08.811.520.224.125.550'], ['E01.370.225.500.223.500', 'E05.200.500.265.500', 'E05.242.223.500', 'E05.481.500.249.500'], ['D27.505.519.389.755'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.550.700'], ['D01.339.431', 'D01.650.775'], ['G02.111.820', 'G04.835'], ['D02.389.678'], ['D02.886.675.933', 'D03.383.129.708.933']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Prevalence and risk factors for hepatitis C virus (HCV) infection among pregnant Brazilian women.
|
OBJECTIVES: To determine the prevalence and the risk factors associated with HCV infection among women at childbirth, and to assess potential for infectivity of anti-HCV-positive women.METHODS: A total of 6995 women were interviewed and screened for HCV antibodies. Association and logistic regression analyses were conducted.RESULTS: The anti-HCV prevalence was 1.5% by EIA-3 and 0.8% by RIBA-3; HCV-RNA (RT-PCR) was detected in 74% of the RIBA-positive samples. Blood transfusion, race (blacks), alcohol abuse, a history of STD and anti-HBc positivity were independent risk factors for HCV positivity. Except for parenteral exposure, independent predictors of anti-HCV were a history of STD, anti-HBc positivity, a sex partner with multiple sex partners and a sex partner with a history of hepatitis.CONCLUSIONS: The prevalence of anti-HCV is higher in pregnant women than in blood donors. Sexual exposure may facilitate the spread of HCV and there is a high potential for mother-to-infant transmission.
|
['Adult', 'Brazil', 'Female', 'Hepacivirus', 'Hepatitis C', 'Humans', 'Logistic Models', 'Pregnancy', 'Pregnancy Complications, Infectious', 'Prevalence', 'RNA, Viral', 'Reverse Transcriptase Polymerase Chain Reaction', 'Risk Factors']
| 10,967,165
|
[['M01.060.116'], ['Z01.107.757.176'], ['B04.450.380', 'B04.820.578.344.475'], ['C01.221.250.750', 'C01.925.440.440', 'C01.925.782.350.350', 'C06.552.380.705.440'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.740.500.525', 'E05.318.740.600.800.450', 'E05.318.740.750.450', 'E05.599.835.875', 'N05.715.360.750.530.480', 'N05.715.360.750.625.700.450', 'N05.715.360.750.695.470', 'N06.850.520.830.500.525', 'N06.850.520.830.600.800.450', 'N06.850.520.830.750.450'], ['G08.686.784.769'], ['C01.674', 'C13.703.700'], ['E05.318.308.985.525.750', 'N01.224.935.597.750', 'N06.850.505.400.975.525.750', 'N06.850.520.308.985.525.750'], ['D13.444.735.828'], ['E05.393.620.500.725'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725']]
|
['Named Groups [M]', 'Geographicals [Z]', 'Organisms [B]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Prevention and therapeutic strategies of thromboembolic events in patients with inflammatory bowel diseases: a report of three cases.
|
Crohn's disease and ulcerative colitis belong to a group of inflammatory bowel diseases (IBD). IBD are characterized by a chronic character of inflammatory process and overlapping immunological abnormalities, which, along with therapeutic strategies are currently available, underlie an increased risk of venous thromboembolic events (VTE). The most common sites of VTE in IBD patients are deep venous thrombosis (DVT) and pulmonary embolism (PE). These complications are particularly important in clinical practice due to a very high mortality rate. Therefore, an early diagnosis of new IBD cases and the control of inflammatory process are thought to play a crucial role in risk reduction for thromboembolic events. Despite considerable evidence supporting the association between IBD and VTE, there is still a lack of recognition of this risk, with dangerous consequences for patients. In this paper authors report three cases of VTE in IBD patients and discuss the most relevant clinical studies found in MEDLINE, Cochrane Library and EMBASE regarding its prevention and management.
|
['Adult', 'Anticoagulants', 'Female', 'Humans', 'Inflammatory Bowel Diseases', 'Venous Thromboembolism']
| 25,382,207
|
[['M01.060.116'], ['D27.505.954.502.119'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C06.405.205.731', 'C06.405.469.432'], ['C14.907.355.590.700']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Pancreatic insulin content regulation by the estrogen receptor ER alpha.
|
The function of pancreatic beta-cells is the synthesis and release of insulin, the main hormone involved in blood glucose homeostasis. Estrogen receptors, ER alpha and ER beta, are important molecules involved in glucose metabolism, yet their role in pancreatic beta-cell physiology is still greatly unknown. In this report we show that both ER alpha and ER beta are present in pancreatic beta-cells. Long term exposure to physiological concentrations of 17beta-estradiol (E2) increased beta-cell insulin content, insulin gene expression and insulin release, yet pancreatic beta-cell mass was unaltered. The up-regulation of pancreatic beta-cell insulin content was imitated by environmentally relevant doses of the widespread endocrine disruptor Bisphenol-A (BPA). The use of ER alpha and ER beta agonists as well as ER alphaKO and ER betaKO mice suggests that the estrogen receptor involved is ER alpha. The up-regulation of pancreatic insulin content by ER alpha activation involves ERK1/2. These data may be important to explain the actions of E2 and environmental estrogens in endocrine pancreatic function and blood glucose homeostasis.
|
['Animals', 'Benzhydryl Compounds', 'Calcium Signaling', 'Estradiol', 'Estrogen Receptor alpha', 'Estrogen Receptor beta', 'Gene Expression Regulation', 'Glucose', 'Insulin', 'Insulin Secretion', 'Islets of Langerhans', 'Male', 'Mice', 'Mice, Inbred C57BL', 'Mice, Knockout', 'Mitogen-Activated Protein Kinase 1', 'Mitogen-Activated Protein Kinase 3', 'Phenols', 'Rats', 'Rats, Wistar']
| 18,446,233
|
[['B01.050'], ['D02.455.426.559.389.115'], ['G02.111.820.800.100', 'G03.143.500.100', 'G04.835.800.100'], ['D04.210.500.365.415.248', 'D06.472.334.851.437.500'], ['D12.776.826.750.350.174'], ['D12.776.826.750.350.262'], ['G05.308'], ['D09.947.875.359.448'], ['D06.472.699.587.200.500.625', 'D12.644.548.586.200.500.625'], ['G03.442', 'G07.475'], ['A03.734.414', 'A06.300.414'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.420', 'B01.050.150.900.649.313.992.635.505.500.400.420'], ['B01.050.050.136.500.500', 'B01.050.150.900.649.313.992.635.505.500.550.455', 'B01.050.150.900.649.313.992.635.505.500.800.500'], ['D08.811.913.696.620.682.700.567.249.500', 'D12.644.360.450.169.500', 'D12.776.476.450.169.500'], ['D08.811.913.696.620.682.700.567.249.750', 'D12.644.360.450.169.750', 'D12.776.476.450.169.750'], ['D02.455.426.559.389.657'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.900']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Relationship between hyperacute blood pressure and outcome after ischemic stroke: data from the VISTA collaboration.
|
BACKGROUND AND PURPOSE: High blood pressure (BP) is associated independently with poor outcome after acute ischemic stroke, although in most analyses "baseline" BP was measured 24 hours or more postictus, and not during the hyperacute period.METHODS: Analyses included 1722 patients in hyperacute trials (recruitment <8 hours) from the Virtual Stroke International Stroke Trial Archive (VISTA) Collaboration. Data on BP at enrollment and after 1, 2, 16, 24, 48, and 72 hours, neurological impairment at 7 days (NIHSS), and functional outcome at 90 days (modified Rankin scale) were assessed using logistic regression models, adjusted for confounding variables; results are for 10-mm Hg change in BP.RESULTS: Mean time to enrollment was 3.7 hours (range 1.0 to 7.9). High systolic BP (SBP) was significantly associated with increased neurological impairment (odds ratio, OR 1.06, 95% confidence interval, 95% CI 1.01 to 1.12), and poor functional outcome; odds ratios for both increased with later BP measurements made at up to 24 hours poststroke. Smaller (versus larger) declines in SBP over the first 24 hours were significantly associated with poor NIHSS scores (OR 1.16, 95% CI 1.05 to 1.27) and functional outcome (OR 1.23, 95% CI 1.13 to 1.34). A large variability in SBP was also associated with poor functional outcome.CONCLUSIONS: High SBP and large variability in SBP in the hyperacute stages of ischemic stroke are associated with increased neurological impairment and poor functional outcome, as are small falls in SBP over the first 24 hours.
|
['Acute Disease', 'Aged', 'Antihypertensive Agents', 'Blood Pressure', 'Brain Ischemia', 'Female', 'Humans', 'Hypertension', 'Logistic Models', 'Male', 'Nervous System Diseases', 'Odds Ratio', 'Stroke', 'Treatment Outcome']
| 19,359,649
|
[['C23.550.291.125'], ['M01.060.116.100'], ['D27.505.954.411.162'], ['E01.370.600.875.249', 'G09.330.380.076'], ['C10.228.140.300.150', 'C14.907.253.092'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C14.907.489'], ['E05.318.740.500.525', 'E05.318.740.600.800.450', 'E05.318.740.750.450', 'E05.599.835.875', 'N05.715.360.750.530.480', 'N05.715.360.750.625.700.450', 'N05.715.360.750.695.470', 'N06.850.520.830.500.525', 'N06.850.520.830.600.800.450', 'N06.850.520.830.750.450'], ['C10'], ['E05.318.740.600.600', 'G17.680.500', 'N05.715.360.750.625.590', 'N06.850.520.830.600.600'], ['C10.228.140.300.775', 'C14.907.253.855'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Diseases [C]', 'Named Groups [M]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Health Care [N]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
A Biodegradation Study of SBA-15 Microparticles in Simulated Body Fluid and in Vivo.
|
Mesoporous silica has received considerable attention as a drug delivery vehicle because of its large surface area and large pore volume for loading drugs and large biomolecules. Recently, mesoporous silica microparticles have shown potential as a three-dimensional vaccine platform for modulating dendritic cells via spontaneous assembly of microparticles in a specific region after subcutaneous injection. For further in vivo applications, the biodegradation behavior of mesoporous silica microparticles must be studied and known. Until now, most biodegradation studies have focused on mesoporous silica nanoparticles (MSNs); here, we report the biodegradation of hexagonally ordered mesoporous silica, SBA-15, with micrometer-sized lengths (?32 ìm with a high aspect ratio). The degradation of SBA-15 microparticles was investigated in simulated body fluid (SBF) and in mice by analyzing the structural change over time. SBA-15 microparticles were found to degrade in SBF and in vivo. The erosion of SBA-15 under biological conditions led to a loss of the hysteresis loop in the nitrogen adsorption/desorption isotherm and fingerprint peaks in small-angle X-ray scattering, specifically indicating a degradation of ordered mesoporous structure. Via comparison to previous results of degradation of MSNs in SBF, SBA-15 microparticles degraded faster than MCM-41 nanoparticles presumably because SBA-15 microparticles have a pore size (?8 nm) and a pore volume larger than those of MCM-41 mesoporous silica. The surface functional groups, the residual amounts of organic templates, and the hydrothermal treatment during the synthesis could affect the rate of degradation of SBA-15. In in vivo testing, previous studies focused on the evaluation of toxicity of mesoporous silica particles in various organs. In contrast, we studied the change in the physical properties of SBA-15 microparticles depending on the duration after subcutaneous injection. The pristine SBA-15 microparticles injected into mice subcutaneously slowly degraded over time and lost ordered structure after 3 days. These findings represent the possible in vivo use of microsized mesoporous silica for drug delivery or vaccine platform after local injection.
|
['Animals', 'Biomimetic Materials', 'Body Fluids', 'Drug Carriers', 'Humans', 'Mice', 'Mice, Inbred BALB C', 'Particle Size', 'Silicon Dioxide', 'Surface Properties']
| 26,013,363
|
[['B01.050'], ['J01.637.087'], ['A12.207'], ['D26.255.260', 'E02.319.300.380'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.338', 'B01.050.150.900.649.313.992.635.505.500.400.338'], ['G02.712'], ['D01.578.750', 'D01.650.550.825', 'D01.837.725'], ['G02.860']]
|
['Organisms [B]', 'Technology, Industry, and Agriculture [J]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Assessing theory of mind nonverbally in those with intellectual disability and ASD: the penny hiding game.
|
Individuals with autism spectrum disorder (ASD) and low intellectual/language abilities are often omitted from experimental studies because of the challenges of testing these individuals. It is vital to develop appropriate and accessible tasks so that this significant part of the spectrum is not neglected. The theory of mind (ToM) has been extensively assessed in ASD, predominantly in relatively high-functioning individuals with reasonable language skills. This study aims to assess the ToM abilities of a sample of 132 participants with intellectual disability (ID) with and without ASD, matched in verbal mental age (VMA) and chronological age, using a naturalistic and nonverbal deception task: the Penny Hiding Game (PHG). The relationship between performance on the PHG and everyday adaptation was also studied. The PHG proved accessible to most participants, suggesting its suitability for use with individuals with low cognitive skills, attentional problems, and limited language. The ASD + ID group showed significantly more PHG errors, and fewer tricks, than the ID group. PHG performance correlated with Vineland adaptation scores for both groups. VMA was a major predictor of passing the task in both groups, and participants with ASD + ID required, on average, 2 years higher VMA than those with ID only, to achieve the same level of PHG success. VMA moderated the association between PHG performance and real-life social skills for the ASD + ID more than the ID group, suggesting that severely impaired individuals with ASD may rely on verbal ability to overcome their social difficulties, whereas individuals with ID alone may use more intuitive social understanding both in the PHG and everyday situations.
|
['Adolescent', 'Adult', 'Aptitude', 'Attention', 'Child', 'Child Development Disorders, Pervasive', 'Child, Preschool', 'Feasibility Studies', 'Female', 'Games, Experimental', 'Humans', 'Intellectual Disability', 'Intelligence', 'London', 'Male', 'Theory of Mind', 'Young Adult']
| 25,258,194
|
[['M01.060.057'], ['M01.060.116'], ['F02.784.629.131'], ['F02.830.104.214'], ['M01.060.406'], ['F03.625.164'], ['M01.060.406.448'], ['E05.318.372.550', 'E05.337.675', 'N05.715.360.330.550', 'N06.850.520.450.550'], ['E05.385'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C10.597.606.360', 'C23.888.592.604.646', 'F01.700.687', 'F03.625.539'], ['F01.752.543'], ['Z01.433.553', 'Z01.542.363.300.553'], ['F02.463.689', 'F02.739.897'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Psychiatry and Psychology [F]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Diseases [C]', 'Geographicals [Z]']
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Using behavioral theory in computer-based health promotion and appraisal.
|
This article explores how behavioral theory can facilitate the development, implementation, and evaluation of health promotion software packages intended to influence personal health practices and/or assess health risks. Current behavioral theories and models are reviewed, and their relevance to developing health promotion software is discussed. A series of six steps is suggested for developing and evaluating health promotion and appraisal software within a behavioral theory framework. These steps should help to facilitate direct application of the theory-based process to health promotion software development.
|
['Behavioral Sciences', 'Computer-Assisted Instruction', 'HIV Infections', 'Health Behavior', 'Health Promotion', 'Humans', 'Microcomputers', 'Sexually Transmitted Diseases', 'Software', 'United States']
| 9,112,096
|
[['F04.096'], ['I02.903.771.500.208'], ['C01.221.250.875', 'C01.221.812.640.400', 'C01.778.640.400', 'C01.925.782.815.616.400', 'C01.925.813.400', 'C20.673.480'], ['F01.145.488'], ['I02.233.332.445', 'N02.421.726.407.579'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['L01.224.230.260.550'], ['C01.221.812', 'C01.778', 'C12.294.668', 'C13.351.500.711', 'C23.550.291.531.937'], ['L01.224.900'], ['Z01.107.567.875']]
|
['Psychiatry and Psychology [F]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Diseases [C]', 'Health Care [N]', 'Organisms [B]', 'Information Science [L]', 'Geographicals [Z]']
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 1
| 1
|
Cytogenetic studies of a series of 43 consecutive secondary myelodysplastic syndromes/acute myeloid leukemias: conventional cytogenetics, FISH, and multiplex FISH.
|
We report a series of 43 consecutive therapy-related myelodysplastic syndromes (t-MDS) or acute myeloid leukemias (t-AML) observed for 6 years. This series consisted of 26 women and 17 men, ages ranging from 9 to 85 years. These cases were classified into three groups according to the primary diagnosis. Conventional cytogenetic and fluorescent in situ hybridization (FISH)/ multiplex FISH (M-FISH) methods were used to analyze cytogenetic characteristics of secondary MDS/AML. The features of chromosomal abnormalities were linked to the nature of the therapy and protocols used. A considerable proportion of recurrent balanced translocations characterized t-AML secondary to therapy. FISH techniques showed that conventional cytogenetics often underestimated associated translocations; some deletions were in fact derivative chromosomes associated with deletions. After treatment for lymphomas and chronic myeloproliferative diseases, there were more complex unbalanced abnormalities than the control group. Compared to other series, recurrent translocations appeared to be more numerous (25%), probably reflecting an evolution of therapeutic modalities.
|
['Adolescent', 'Adult', 'Aged', 'Aged, 80 and over', 'Child', 'Female', 'Gene Amplification', 'Gene Deletion', 'Humans', 'In Situ Hybridization, Fluorescence', 'Karyotyping', 'Leukemia, Myeloid, Acute', 'Male', 'Middle Aged', 'Myelodysplastic Syndromes', 'Translocation, Genetic']
| 16,843,103
|
[['M01.060.057'], ['M01.060.116'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['M01.060.406'], ['G05.308.250', 'G05.365.590.310', 'G05.558.315'], ['G05.365.590.762.320', 'G05.558.800.320'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.225.500.620.670.325.350', 'E01.370.225.750.600.670.325.350', 'E05.200.500.620.670.325.350', 'E05.200.750.600.670.325.350', 'E05.393.285.350', 'E05.393.661.475.350'], ['E01.370.225.500.385.315', 'E05.200.500.385.315', 'E05.242.385.315', 'E05.393.285.475'], ['C04.557.337.539.275'], ['M01.060.116.630'], ['C15.378.190.625'], ['C23.550.210.870', 'G05.365.590.175.870', 'G05.558.860']]
|
['Named Groups [M]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Reliability and Validity of the Daily Parent Rating of Evening and Morning Behavior Scale, Revised.
|
OBJECTIVE: Children with ADHD frequently manifest behavioral difficulties in the morning prior to school. We sought to assess the reliability and validity of the Daily Parent Rating of Evening and Morning Behavior Scale, Revised (DPREMB-R) morning score as a measure of morning behaviors impaired by ADHD.METHOD: We used data from a clinical trial of HLD200 treatment in pediatric participants with ADHD to address our objectives.RESULTS: The DPREMB-R morning score showed significant internal homogeneity, test-retest reliability ( r = .52-.45), and good concurrent validity ( r = .50-.71).CONCLUSION: The DPREMB-R morning score could be a useful instrument for assessing treatment efficacy in the morning before school.
|
['Adolescent', 'Adrenergic Uptake Inhibitors', 'Atomoxetine Hydrochloride', 'Attention Deficit Disorder with Hyperactivity', 'Behavior Rating Scale', 'Child', 'Child Behavior Disorders', 'Child, Preschool', 'Double-Blind Method', 'Female', 'Humans', 'Male', 'Parents', 'Reproducibility of Results', 'Schools', 'Time Factors']
| 26,700,792
|
[['M01.060.057'], ['D27.505.519.562.437.050', 'D27.505.519.625.050.601', 'D27.505.519.625.600.050', 'D27.505.696.577.050.601', 'D27.505.696.577.600.050'], ['D02.092.831.085'], ['F03.625.094.150'], ['F04.711.271'], ['M01.060.406'], ['F03.625.141'], ['M01.060.406.448'], ['E05.318.370.300', 'E05.581.500.300', 'N05.715.360.325.320', 'N06.850.520.445.300'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F01.829.263.500.320', 'I01.880.853.150.500.340', 'M01.620'], ['E05.318.370.725', 'E05.337.851', 'N05.715.360.325.685', 'N06.850.520.445.725'], ['I02.783', 'J03.832'], ['G01.910.857']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Psychiatry and Psychology [F]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Technology, Industry, and Agriculture [J]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 0
|
Falling through the regulatory cracks: Street selling of pesticides and poisoning among urban youth in South Africa.
|
In South Africa, as in much of the developing world, youth participation in the informal, unregulated street pesticide market results in exposures and risks of acute and chronic effects, yet has gone largely undocumented. A conceptual framework for understanding youth involvement in street pesticide sales and use includes contextual factors, health outcomes, and externalities (unintended negative consequences). An exploratory study based on this framework shows that highly-toxic pesticides, such as aldicarb, methamidophos, and chlorpyrifos, are easily available in informal markets in Cape Town's urban periphery. Youth are involved in the sale, distribution, and use of street pesticides, and are exposed during handling, transportation, spillage, storage, use and other activities, with little safety information available. Demand and supply for street pesticides is driven by joblessness, poverty, and inadequate pest management strategies. National and international efforts addressing underlying contextual determinants are required to protect children from exposures to street pesticides.
|
['Adolescent', 'Adult', 'Aldicarb', 'Female', 'Human Rights', 'Humans', 'Male', 'Middle Aged', 'Occupational Exposure', 'Organophosphate Poisoning', 'Pesticides', 'Population Surveillance', 'Pyrethrins', 'South Africa', 'Urban Population']
| 20,465,065
|
[['M01.060.057'], ['M01.060.116'], ['D02.241.081.251.046'], ['I01.880.604.473', 'N03.706.437'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['N06.850.460.350.600'], ['C25.723.717'], ['D27.720.031.700', 'D27.888.723'], ['E05.318.308.980.438.700', 'N05.715.360.300.800.438.625', 'N06.850.520.308.980.438.700', 'N06.850.780.675'], ['D02.455.849.575.188.750'], ['Z01.058.290.175.735'], ['N01.600.900']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]', 'Organisms [B]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Geographicals [Z]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 1
|
The breast dynamic contrast enhanced MRI: preliminary results of a quantitative analysis.
|
The objective of our study was to apply a quantitative analysis to the dynamic contrast enhanced MR imaging of the breast. Automated criteria increase the objectivity and reproducibility of the diagnostic interpretation of the imaging for differentiating benign and malignant lesions. The validation of this applied method was evaluated by analysing the time- signal intensity curves and the performance of the extracted enhancement parameters. The performance of some extracted parameters was evaluated by ROC (Receiver Operating Characteristic) analysis. These parameters were found to be particularly accurate in differentiating lesions.
|
['Breast', 'Breast Neoplasms', 'Contrast Media', 'Female', 'Gadolinium DTPA', 'Humans', 'Magnetic Resonance Imaging', 'Software']
| 17,725,104
|
[['A01.236'], ['C04.588.180', 'C17.800.090.500'], ['D27.505.259.500', 'D27.720.259'], ['D02.092.782.590.401', 'D02.241.081.018.639.400', 'D02.257.141'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.350.825.500'], ['L01.224.900']]
|
['Anatomy [A]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Information Science [L]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Electromyographic evaluation in children orthodontically treated for skeletal Class II malocclusion: Comparison of two treatment techniques.
|
OBJECTIVE: To compare the clinical efficacy of two techniques for fabricating a Bimler device by assessing the patient's surface electromyography (sEMG) activity at rest before treatment and six months after treatment.METHODS: Twenty-four patients undergoing orthodontic treatment were enrolled in the study; 12 formed the test group and wore a Bimler device fabricated with a Myoprint impression using neuromuscular orthodontic technique and 12 formed the control group and were treated by traditional orthodontic technique with a wax bite in protrusion. The "rest" sEMG of each patient was recorded prior to treatment and six months after treatment.RESULTS: The neuromuscular-designed Bimler device was more comfortable and provided better treatment results than the traditional Bimler device.CONCLUSION: This study suggests that the patient group subjected to neuromuscular orthodontic treatment had a treatment outcome with more relaxed masticatory muscles and better function versus the traditional orthodontic treatment.
|
['Child', 'Electromyography', 'Female', 'Humans', 'Male', 'Malocclusion, Angle Class II', 'Masticatory Muscles', 'Orthodontic Appliance Design', 'Orthodontic Appliances, Functional', 'Orthodontics', 'Treatment Outcome']
| 29,143,566
|
[['M01.060.406'], ['E01.370.405.255', 'E01.370.530.255'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C07.793.494.630'], ['A02.633.567.600', 'A14.530'], ['E05.320.274', 'E06.658.450', 'E06.912.675'], ['E06.658.453.560'], ['E06.658', 'H02.163.876.439'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Diseases [C]', 'Anatomy [A]', 'Disciplines and Occupations [H]', 'Health Care [N]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 1
| 0
|
HIV behavioural risks and the role of work environment among Chinese male sex workers in Hong Kong.
|
Male sex workers are a highly marginalised group in Hong Kong and it is increasingly so with an influx of them travelling from mainland China to work as "freelance" sex workers. This study aimed to measure important work environment variables that might affect the likelihood of condom use among male sex workers working in Hong Kong. A cross-sectional survey of 161 participants recruited by snowball and convenience sampling methods through outreach workers of a local non-governmental organization was conducted in 2007-2008. Only 27.4%, 54.7% and 42.6% reported consistent condom use when engaging in oral, anal and vaginal sex, respectively. Logistic regression shows unsafe sex was nearly four times (OR=3.41; 95%CI 1.51-7.69) as common in institutionalised male sex workers as among their independent counterparts. Lack of condoms provided at workplaces was a major barrier in this socio-legal context and was strongly associated with condom non-use amongst institutionalised sex workers (OR= 10.86; 95%CI 2.94-40.17). The present study finds that when compared with independent Male sex workers (MSWs), institutionalised MSWs were older, less educated, earned a higher income but more likely to engage in unsafe sex with their clients and their partners. Public health physicians must work with law-enforcing authorities to provide clear guidelines to remove these HIV prevention barriers.
|
['Adult', 'China', 'Condoms', 'Cross-Sectional Studies', 'HIV Infections', 'Health Knowledge, Attitudes, Practice', 'Hong Kong', 'Humans', 'Male', 'Middle Aged', 'Risk Factors', 'Risk-Taking', 'Safe Sex', 'Sex Work', 'Sex Workers', 'Sexual Behavior', 'Unsafe Sex', 'Workplace', 'Young Adult']
| 22,293,067
|
[['M01.060.116'], ['Z01.252.474.164'], ['E07.190.270.150'], ['E05.318.372.500.875', 'N05.715.360.330.500.875', 'N06.850.520.450.500.875'], ['C01.221.250.875', 'C01.221.812.640.400', 'C01.778.640.400', 'C01.925.782.815.616.400', 'C01.925.813.400', 'C20.673.480'], ['F01.100.150.500', 'N05.300.150.410'], ['Z01.252.474.164.450'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['F01.145.722'], ['F01.145.802.845'], ['F01.145.802.790', 'I01.880.735.679'], ['M01.776'], ['F01.145.802'], ['F01.145.802.987'], ['N01.824.245.925', 'N04.452.677.975'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Organisms [B]', 'Anthropology, Education, Sociology, and Social Phenomena [I]']
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 1
|
Selected neurophysiological, psychological, and behavioral influences on subjective sleep quality in nurses: a structure equation model.
|
Few studies have examined relationships among neurophysiological, psychological, and behavioral factors with regard to their effects on sleep quality. We used a structure equation model to investigate behavioral and psychological factors that influence neurophysiological regulation of sleep in shift workers. Using a cross-sectional study design, we tested the model with a sample of 338 female nurses working rotating shifts at an urban regional hospital. The Morningness-Eveningness Questionnaire (MEQ) and short-form Menstrual Distress Questionnaire (MDQ) were used to measure neurophysiological factors involved in morningness-eveningness and menstrual distress. The Sleep Hygiene Awareness and Practice Scale (SHAPS) and Profile of Mood States Short Form (POMS-SF) were completed to measure behavioral factors of sleep hygiene practices and psychological factors of mood states. In addition, the Pittsburgh Sleep Quality Index (PSQI) measured participant's self-reported sleep quality. The results revealed that sleep hygiene practices and mood states mediated the effects of morningness-eveningness and menstrual distress on sleep quality. Our findings provide support for developing interventions to enhance sleep hygiene and maintain positive mood states to reduce the influence of neurophysiological factors on sleep quality among shift workers.
|
['Adult', 'Cross-Sectional Studies', 'Female', 'Humans', 'Nurses', 'Sleep', 'Surveys and Questionnaires', 'Work Schedule Tolerance', 'Young Adult']
| 24,278,145
|
[['M01.060.116'], ['E05.318.372.500.875', 'N05.715.360.330.500.875', 'N06.850.520.450.500.875'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.526.485.650', 'N02.360.650'], ['F02.830.855', 'G11.561.803'], ['E05.318.308.980', 'N05.715.360.300.800', 'N06.850.520.308.980'], ['I03.946.225.375', 'N04.452.677.650.375'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]', 'Anthropology, Education, Sociology, and Social Phenomena [I]']
| 0
| 1
| 0
| 0
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 0
|
Enhanced information retrieval from narrative German-language clinical text documents using automated document classification.
|
The amount of narrative clinical text documents stored in Electronic Patient Records (EPR) of Hospital Information Systems is increasing. Physicians spend a lot of time finding relevant patient-related information for medical decision making in these clinical text documents. Thus, efficient and topical retrieval of relevant patient-related information is an important task in an EPR system. This paper describes the prototype of a medical information retrieval system (MIRS) for clinical text documents. The open-source information retrieval framework Apache Lucene has been used to implement the prototype of the MIRS. Additionally, a multi-label classification system based on the open-source data mining framework WEKA generates metadata from the clinical text document set. The metadata is used for influencing the rank order of documents retrieved by physicians. Combining information retrieval and automated document classification offers an enhanced approach to let physicians and in the near future patients define their information needs for information stored in an EPR. The system has been designed as a J2EE Web-application. First findings are based on a sample of 18,000 unstructured, clinical text documents written in German.
|
['Abstracting and Indexing', 'Austria', 'Database Management Systems', 'Documentation', 'Hospital Information Systems', 'Humans', 'Information Storage and Retrieval', 'Internet', 'Language', 'Medical Records Systems, Computerized', 'Narration', 'Natural Language Processing', 'Software', 'Unified Medical Language System', 'Vocabulary, Controlled']
| 18,487,776
|
[['L01.453.245.100'], ['Z01.542.088'], ['L01.224.068', 'L01.224.900.280', 'N04.452.515.110'], ['L01.453.245'], ['N04.452.442.452.452', 'N04.452.515.360'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['L01.313.500.750.280', 'L01.470'], ['L01.224.230.110.500'], ['F01.145.209.399', 'L01.559'], ['E05.318.308.940.968.625', 'L01.313.500.750.300.695', 'N04.452.859.564.650', 'N05.715.360.300.715.500.530', 'N06.850.520.308.940.968.625'], ['E05.318.308.502', 'F01.145.209.459', 'L01.399.250.660', 'N05.715.360.300.480', 'N06.850.520.308.502'], ['L01.224.050.375.580'], ['L01.224.900'], ['L01.453.245.945.800'], ['L01.453.245.945']]
|
['Information Science [L]', 'Geographicals [Z]', 'Health Care [N]', 'Organisms [B]', 'Psychiatry and Psychology [F]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 1
| 1
|
Neurovascular in-stent stenoses: treatment with conventional and drug-eluting balloons.
|
BACKGROUND AND PURPOSE: ISRs remain a major issue in the endovascular management of ICAD, requiring retreatment by reangioplasty. The aim of the present study was to evaluate the technical feasibility, safety, and efficiency of the novel DEBs for neurovascular ISRs.MATERIALS AND METHODS: Fifty-one patients (median age, 67 years; age range, 34-82 years; male/female ratio, 37:14) underwent 63 balloon dilation procedures for ISRs in intracranial stented arterial segments between November 2007 and August 2010 in a single center. Of the 63 procedures, 20 (32%) were performed by using a conventional balloon and 43 (68%), by using a paclitaxel-eluting balloon (SeQuent Please). Angiographic and clinical follow-up was performed at 6 and 12 weeks, 6 and 12 months, and yearly thereafter. Technical success rate, periprocedural complications, occurrence of recurrent ischemic symptoms, and the development of a recurrent ISR after reangioplasty were analyzed.RESULTS: Technical success, defined as <50% residual stenosis was achieved in all cases (100%), with failure of the DEB treatment in 6% of the attempts; those lesions were finally successfully treated with a conventional balloon. The combined permanent neurologic morbidity and mortality rate (stroke, ICH, and SAH) at 30 days was 1.6%. Substantial difference was found in the rate of recurrent stenosis when comparing conventional balloons and DEBs, with recurrent stenosis rates of 50% and 9%, respectively.CONCLUSIONS: The initial results of reangioplasty of intracranial ISRs with DEBs are encouraging; further technical developments are, nevertheless, mandatory.
|
['Adult', 'Aged', 'Catheterization', 'Cerebrovascular Disorders', 'Combined Modality Therapy', 'Drug-Eluting Stents', 'Female', 'Graft Occlusion, Vascular', 'Humans', 'Male', 'Middle Aged', 'Paclitaxel', 'Radiography', 'Treatment Outcome', 'Tubulin Modulators']
| 21,885,715
|
[['M01.060.116'], ['M01.060.116.100'], ['E02.148', 'E05.157'], ['C10.228.140.300', 'C14.907.253'], ['E02.186'], ['E07.695.750.500'], ['C23.550.767.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['D02.455.426.392.368.242.888.777', 'D02.455.849.291.850.777'], ['E01.370.350.700'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['D27.505.519.593.249.500']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Health Care [N]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Fine Astrocyte Processes Contain Very Small Mitochondria: Glial Oxidative Capability May Fuel Transmitter Metabolism.
|
The peripheral astrocyte process (PAP) is the glial compartment largely handling inactivation of transmitter glutamate, and supplying glutamate to the axon terminal. It is not clear how these energy demanding processes are fueled, and whether the PAP exhibits oxidative capability. Whereas the GFAP-positive perinuclear cytoplasm and stem process are rich in mitochondria, the PAP is often considered too narrow to contain mitochondria and might thus not rely on oxidative metabolism. Applying high resolution light microscopy, we investigate here the presence of mitochondria in the PAPs of freshly dissociated, isolated astrocytes. We provide an overview of the subcellular distribution and the approximate size of astrocytic mitochondria. A substantial proportion of the astrocyte's mitochondria are contained in the PAPs and, on the average, they are smaller there than in the stem processes. The majority of mitochondria in the stem and peripheral processes are surprisingly small (0.2-0.4 µm), spherical and not elongate, or tubular, which is supported by electron microscopy. The density of mitochondria is two to several times lower in the PAPs than in the stem processes. Thus, PAPs do not constitute a mitochondria free glial compartment but contain mitochondria in large numbers. No juxtaposition of mitochondria-containing PAPs and glutamatergic synapses has been reported. However, the issue of sufficient ATP concentrations in perisynaptic PAPs can be seen in the light of (1) the rapid, activity dependent PAP motility, and (2) the recently reported activity-dependent mitochondrial transport and immobilization leading to spatial, subcellular organisation of glutamate uptake and oxidative metabolism.
|
['Adenosine Triphosphate', 'Animals', 'Astrocytes', 'Glial Fibrillary Acidic Protein', 'Mitochondria', 'Mitochondrial Size', 'Neural Stem Cells', 'Neuroglia', 'Neurotransmitter Agents', 'Oxidation-Reduction', 'Peripheral Nervous System', 'Primary Cell Culture', 'Rats', 'Subcellular Fractions', 'Synapses']
| 25,894,677
|
[['D03.633.100.759.646.138.236', 'D13.695.667.138.236', 'D13.695.827.068.236'], ['B01.050'], ['A08.637.200', 'A11.650.200'], ['D05.750.078.593.400', 'D12.776.220.475.400'], ['A11.284.430.214.190.875.564', 'A11.284.835.626'], ['G04.670.560'], ['A11.872.653'], ['A08.637', 'A11.650'], ['D27.505.519.625', 'D27.505.696.577'], ['G02.700', 'G03.295.531'], ['A08.800'], ['E01.370.225.500.223.500', 'E05.200.500.265.500', 'E05.242.223.500', 'E05.481.500.249.500'], ['B01.050.150.900.649.313.992.635.505.700'], ['A11.284.835'], ['A08.850', 'A11.284.149.165.420.780']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
The effect of Coenzyme Q10 on reperfusion injury in canine myocardium.
|
The mechanism of mitochondrial damage during reperfusion injury of ischemic myocardium was studied using mongrel dogs in vivo and isolated mitochondria in vitro. Seventy-seven adult dogs were divided into three groups: the control group (n = 38), the Coenzyme Q10 (CoQ10)-5 mg group (n = 24), and the CoQ10-15 mg group (n = 15). In the control group, the left anterior descending coronary artery (LAD) of the dog was occluded for 15 min followed by 5 min of reperfusion after 40 min of premedication with physiological saline. In both CoQ10 groups, 5 mg/kg or 15 mg/kg of CoQ10 was infused intravenously for 20 min and then physiological saline was administered for 20 min before 15 min occlusion of the LAD. Subsequently, reperfusion was allowed for 5 min. Each group was further divided into two subgroups depending on the presence (arrhythmia group) or the absence (non-arrhythmia group) of ventricular arrhythmias. Immediately after 15 min occlusion, myocardial samples were taken from the normal and reperfused areas to measure CoQ10 content of myocardium. Heart mitochondria were prepared after 5 min of reperfusion from both areas. Arrhythmias appeared in 12 of 38 dogs in the control group (32%), two of 24 dogs in the CoQ10-5 mg group (8%) and none of 15 dogs in the CoQ10-15 mg group (0%). Premedication with CoQ10 increased tissue CoQ10 content in a dose-dependent manner. In the CoQ10-5 mg group, the increase in CoQ10 content of dogs with reperfusion arrhythmias was relatively less than that of dogs without reperfusion arrhythmias. In each group, mitochondrial function was decreased in the arrhythmia group compared to that of the non-arrhythmia group. The increase in free fatty acid (FFA) content and the decrease in phospholipid content were also observed in mitochondria from the reperfused area of each arrhythmia group. The increase in FFA and mitochondrial dysfunction were induced by the incubation of mitochondria in vitro with phospholipase (PLase) A2 or PLase C, and protected by the addition of CoQ10. These results suggest that PLase plays an important role in the development of mitochondrial damage associated with reperfusion.
|
['Animals', 'Arrhythmias, Cardiac', 'Coenzymes', 'Coronary Circulation', 'Dogs', 'Fatty Acids', 'Mitochondria, Heart', 'Myocardial Infarction', 'Oxygen Consumption', 'Phospholipases', 'Phospholipases A', 'Ubiquinone']
| 4,046,048
|
[['B01.050'], ['C14.280.067', 'C23.550.073'], ['D08.211'], ['G09.330.100.324'], ['B01.050.150.900.649.313.750.250.216.200'], ['D10.251'], ['A11.284.430.214.190.875.564.627.603', 'A11.284.835.626.627.603'], ['C14.280.647.500', 'C14.907.585.500', 'C23.550.513.355.750', 'C23.550.717.489.750'], ['G03.680'], ['D08.811.277.352.100.680', 'D08.811.277.352.640.700'], ['D08.811.277.352.100.680.750'], ['D02.806.250.900', 'D08.211.935']]
|
['Organisms [B]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Recognition of peer emotions in children with ADHD: Evidence from an animated facial expressions task.
|
A growing body of literature suggests that ADHD is associated with emotion recognition impairments that may be linked to deficient interpersonal functioning. However, our understanding of the mechanisms underlying these recognition impairments is extremely limited. Here, we used dynamic stimuli to investigate whether impaired emotion recognition in children with ADHD may be associated with impairments in perceptual sensitivity. Participants (ADHD: N = 26; Controls: N = 26) viewed video sequences of neutral faces slowly developing into one of the six basic emotional expressions (angry, happy, fearful, sad, disgusted and surprised) and were instructed to indicate via a button press the precise moment at which they were able to correctly recognize the emotional expression. The results showed that compared to controls, children with ADHD exhibited lower accuracy rates across all emotional expressions while there was no evidence for impaired perceptual sensitivity. Thus, the study provides evidence for a generalized categorization impairment across all emotional categories and is consistent with developmental delay accounts of ADHD. Future studies are needed in order to further investigate the developmental course of social cognition deficits in ADHD.
|
['Adolescent', 'Anger', 'Attention Deficit Disorder with Hyperactivity', 'Child', 'Emotions', 'Facial Expression', 'Facial Recognition', 'Fear', 'Female', 'Happiness', 'Humans', 'Male', 'Peer Group']
| 28,917,441
|
[['M01.060.057'], ['F01.470.093'], ['F03.625.094.150'], ['M01.060.406'], ['F01.470'], ['E01.370.600.225', 'F01.145.209.530.385'], ['F02.463.593.524.250.500', 'F02.463.593.524.500.500', 'F02.463.593.932.622.500'], ['F01.470.361'], ['F01.470.516'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F01.829.316.483']]
|
['Named Groups [M]', 'Psychiatry and Psychology [F]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Thermal Suit or Forced Air Warming in Prevention of Perioperative Hypothermia: A Randomized Controlled Trial.
|
PURPOSE: To prevent perioperative hypothermia, forced air warming blanket was compared with a passive insulation suit.DESIGN: Prospective, open, randomized controlled trial.METHODS: Thirty patients were scheduled for orthopedic spinal surgery. The intervention group (group TS) received the thermal suit T-Balance before premedication and throughout the perioperative period, whereas the control group (group C) received forced air warming (FAW) during surgery.FINDINGS: No statistically significant difference (ns) was found between the groups for core temperature 30 minutes after induction of general anesthesia. Perioperative hypothermia occurred in 10 (66.7%) patients in group TS and 6 (40%) in group C (ns). For hypothermic patients, re-establishment of normothermia took significantly longer in group TS, mean 108 ± 111 minutes, than in group C, 33 ± 59.5 minutes (P = .03).CONCLUSIONS: The thermal suit did not prevent hypothermia in this study. FAW was significantly more efficient in re-establishing normothermia.
|
['Adult', 'Bedding and Linens', 'Body Temperature Regulation', 'Female', 'Humans', 'Hypothermia', 'Male', 'Middle Aged', 'Orthopedic Procedures', 'Perioperative Period', 'Prospective Studies', 'Sweden', 'Time Factors']
| 31,204,273
|
[['M01.060.116'], ['E07.325.137', 'J01.494.221'], ['G07.110.232', 'G07.410.421', 'G16.012.500.535'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C23.888.119.565'], ['M01.060.116.630'], ['E02.718', 'E04.555'], ['E04.614', 'N02.421.585.753'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['Z01.542.816.500'], ['G01.910.857']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Technology, Industry, and Agriculture [J]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Diseases [C]', 'Health Care [N]', 'Geographicals [Z]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 1
| 1
|
Immunohistochemical detection of Ki67 in breast cancer correlates with transcriptional regulation of genes related to apoptosis and cell death.
|
Ki67 is a nuclear protein that is tightly linked to the cell cycle. It is a marker of cell proliferation and has been used to stratify good and poor prognostic categories in invasive breast cancer. Its correlation with gene expression patterns has not been fully elucidated. In this study, Ki67 immunohistochemistry using the MIB-1 antibody was performed on sections cut from 21 formalin-fixed, paraffin-embedded invasive breast cancers. Scoring was determined as nil (no immunostaining), low (10% or less immunopositivity) or high (>10% immunoreactive cells) respectively. The relationship of Ki67 immunohistochemical detection with clinicopathologic parameters was evaluated. Using Affymetrix U133A GeneChips, expression profiles for these tumors were generated and correlated with Ki67 immunohistochemical findings. Analysis of variance was used to define genes that were differentially regulated between the groups. Real-time polymerase chain reaction (PCR) was used to confirm the presence of a downregulated gene. Our results showed high, low and nil Ki67 immunostaining in nine (43%), six (28.5%) and six (28.5%) invasive breast cancers respectively, with increased Ki67 protein expression correlating with high histologic grade (P=0.02), mitotic score (P=0.001) and estrogen receptor immunonegativity (P=0.002). Expression profiling trends of the Ki67 gene mirrored the observed proportions of immunostained cells when the Ki67 immunoscore was >10%. Genes related to apoptosis and cell death (bcl2, MAP2K4, TNF10) were noted to be downregulated in tumors that disclosed >40% Ki67 immunostaining (P<0.001). Downregulation of the bcl2 gene was confirmed at the RNA level by real-time RT-PCR. Differential regulation of these genes, especially bcl2, may contribute to the biological nature of clinically more aggressive and highly proliferative breast cancers.
|
['Adult', 'Aged', 'Analysis of Variance', 'Apoptosis', 'Apoptosis Regulatory Proteins', 'Breast Neoplasms', 'Cell Death', 'Down-Regulation', 'Female', 'Gene Expression Regulation, Neoplastic', 'Humans', 'Immunohistochemistry', 'Ki-67 Antigen', 'MAP Kinase Kinase 4', 'Membrane Glycoproteins', 'Middle Aged', 'Proto-Oncogene Proteins c-bcl-2', 'RNA, Messenger', 'Reverse Transcriptase Polymerase Chain Reaction', 'TNF-Related Apoptosis-Inducing Ligand', 'Transcription, Genetic', 'Tumor Necrosis Factor-alpha', 'Up-Regulation']
| 15,578,079
|
[['M01.060.116'], ['M01.060.116.100'], ['E05.318.740.150', 'N05.715.360.750.125', 'N06.850.520.830.150'], ['G04.146.954.035'], ['D12.644.360.075', 'D12.776.476.075'], ['C04.588.180', 'C17.800.090.500'], ['G04.146'], ['G02.111.240', 'G05.308.200', 'G07.690.773.937'], ['G05.308.370'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.225.500.607.512', 'E01.370.225.750.551.512', 'E05.200.500.607.512', 'E05.200.750.551.512', 'E05.478.583', 'H01.158.100.656.234.512', 'H01.158.201.344.512', 'H01.158.201.486.512', 'H01.181.122.573.512', 'H01.181.122.605.512'], ['D12.776.660.625.500', 'D23.050.290.500', 'D23.101.140.400'], ['D08.811.913.696.620.682.700.565.400', 'D08.811.913.696.620.682.725.200.400', 'D12.644.360.440.400', 'D12.776.476.440.400'], ['D12.776.395.550', 'D12.776.543.550'], ['M01.060.116.630'], ['D12.644.360.075.718', 'D12.776.476.075.718', 'D12.776.624.664.700.169'], ['D13.444.735.544'], ['E05.393.620.500.725'], ['D12.644.276.374.750.625', 'D12.776.467.374.750.625', 'D23.529.374.750.625'], ['G02.111.873', 'G05.297.700'], ['D12.644.276.374.500.800', 'D12.644.276.374.750.626', 'D12.776.124.900', 'D12.776.395.930', 'D12.776.467.374.500.800', 'D12.776.467.374.750.626', 'D23.529.374.500.800', 'D23.529.374.750.626'], ['G02.111.905', 'G05.308.850', 'G07.690.773.998']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Organisms [B]', 'Disciplines and Occupations [H]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 1
| 1
| 0
|
Prostate Specific Antigen-Positive Deceased Organ Donor: A Pathologist Is Indispensable.
|
BACKGROUND: Due to demographic projections, and lack of an algorithm in the case of a prostate specific antigen (PSA)-positive donor, the loss of organ recovery may occur more frequently in the near future without approved procedures. In Poland in recent years it has been recommended to determine tumor markers in potential donors. In the first year of the recommendation 10% of potential deceased donors were disqualified in our transplantation center on the basis of the elevated PSA levels (high PSA >10 ng/mL). Histopathologic evaluation of prostate was implemented in a donor qualification procedure to prevent reduction of the actual organ donor pool.MATERIAL AND METHODS: In the period of January 2010-January 2014 each donor reported to a coordination center (n = 52; median age, 54 years) and underwent the routine histological evaluation of the whole prostate, regardless of the PSA level.RESULTS: Pathologist revealed in the study group of 52 male donors, 6 cases of carcinoma of the prostate (CaP; 12%). There was no correlation between PSA level and CaP (-)/CaP(+) (median 7.0 vs 3.9 ng/mL, respectively; P = .51) nor high-grade prostate intraepithelial neoplasia (HGPIN) (+)/HGPIN (-) (median 5.9 vs 4.3 ng/mL; P = .14). All of the recovered organs (12 kidneys and 3 livers) from donors with CaP were transplanted, resulting in a 15% increase in the organ donor pool.CONCLUSIONS: There is no association between PSA values and CaP occurrence in deceased organ donors. Histological verification allowed for an increase in the organ pool with maintenance of safety standards.
|
['Adult', 'Aged', 'Algorithms', 'Biomarkers, Tumor', 'Clinical Competence', 'Humans', 'Male', 'Middle Aged', 'Pathologists', 'Poland', 'Prostate', 'Prostate-Specific Antigen', 'Prostatic Intraepithelial Neoplasia', 'Prostatic Neoplasms', 'Tissue Donors', 'Tissue and Organ Procurement', 'Young Adult']
| 27,742,319
|
[['M01.060.116'], ['M01.060.116.100'], ['G17.035', 'L01.224.050'], ['D23.101.140'], ['I02.399.630.210', 'N04.761.210', 'N05.715.175'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['M01.526.485.810.746', 'N02.360.810.746'], ['Z01.542.248.679'], ['A05.360.444.575', 'A10.336.707'], ['D08.811.277.656.300.760.442.750', 'D08.811.277.656.959.350.442.750', 'D12.776.866.249.500', 'D23.050.285.625', 'D23.101.140.625'], ['C04.557.470.200.240.500'], ['C04.588.945.440.770', 'C12.294.260.750', 'C12.294.565.625', 'C12.758.409.750'], ['M01.898'], ['N02.421.911'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Chemicals and Drugs [D]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]', 'Organisms [B]', 'Geographicals [Z]', 'Anatomy [A]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 1
| 1
| 1
| 1
|
An overview of the CERC ARTEMIS project.
|
The basic premise of this effort is that health care can be made more effective and affordable by applying modern computer technology to improve collaboration among diverse and distributed health care providers. Information sharing, communication, and coordination are basic elements of any collaborative endeavor. In the health care domain, collaboration is characterized by cooperative activities by health care providers to deliver total and real-time care for their patients. Communication between providers and managed access to distributed patient records should enable health care providers to make informed decisions about their patients in a timely manner. With an effective medical information infrastructure in place, a patient will be able to visit any health care provider with access to the network, and the provider will be able to use relevant information from even the last episode of care in the patient record. Such a patient-centered perspective is in keeping with the real mission of health care providers. Today, an easy-to-use, integrated health care network is not in place in any community, even though current technology makes such a network possible. Large health care systems have deployed partial and disparate systems that address different elements of collaboration. But these islands of automation have not been integrated to facilitate cooperation among health care providers in large communities or nationally. CERC and its team members at Valley Health Systems, Inc., St. Marys Hospital and Cabell Huntington Hospital form a consortium committed to improving collaboration among the diverse and distributed providers in the health care arena. As the first contract recipient of the multi-agency High Performance Computing and Communications (HPCC) Initiative, this team of computer system developers, practicing rural physicians, community care groups, health care researchers, and tertiary care providers are using research prototypes and commercial off-the-shelf technologies to develop an open collaboration environment for the health care domain. This environment is called ARTEMIS--Advanced Research TEstbed for Medical InformaticS.
|
['Computer Communication Networks', 'Computer Security', 'Computer Systems', 'Hospital Information Systems', 'Humans', 'Medical Records Systems, Computerized', 'Systems Integration', 'Telemedicine']
| 8,563,249
|
[['L01.224.230.110'], ['L01.224.134', 'N04.452.910.200'], ['L01.224.230'], ['N04.452.442.452.452', 'N04.452.515.360'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.308.940.968.625', 'L01.313.500.750.300.695', 'N04.452.859.564.650', 'N05.715.360.300.715.500.530', 'N06.850.520.308.940.968.625'], ['H01.770.787', 'L01.906.787'], ['H02.403.840', 'L01.178.847.652', 'N04.590.374.800']]
|
['Information Science [L]', 'Health Care [N]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]']
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 0
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Protective mechanism of Wnt4 gene on Parkinson's disease (PD) transgenic Drosophila.
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OBJECTIVE: The main pathological change of Parkinson's disease (PD) is progressive degeneration and necrosis of dopaminergic neurons in the midbrain, forming a Lewy body in many of the remaining neurons. Studies have found that in transgenic Drosophila, mutations in the PTEN-inducible kinase 1 (PINK1) gene may cause indirect flight muscle defects in Drosophila, and mitochondrial structural dysfunction as well.METHODS: In this study, Wnt4 gene overexpression and knockdown were performed in PINK1 mutant PD transgenic Drosophila, and the protective effect of Wnt4 gene on PD transgenic Drosophila and its possible mechanism were explored. The Wnt4 gene was screened in the previous experiment; And by using the PD transgenic Drosophila model of the MHC-Gal4/UAS system, the PINK1 gene could be specifically activated in the Drosophila muscle tissue.RESULTS: In PINK1 mutation transgenic fruit flies, the Wnt4 gene to study its implication on PD transgenic fruit flies' wing normality and flight ability. We found that overexpression of Wnt4 gene significantly reduced abnormality rate of PD transgenic Drosophila and improved its flight ability, and then, increased ATP concentration, enhanced mitochondrial membrane potential and normalized mitochondrial morphology were found. All of these findings suggested Wnt4 gene may have a protective effect on PD transgenic fruit flies. Furthermore, in Wnt4 gene overexpression PD transgenic Drosophila, down-regulation autophagy and apoptosis-related proteins Ref(2)P, Pro-Caspase3, and up-regulation of Beclin1, Atg8a, Bcl2 protein were confirmed by Western Blotting.CONCLUSION: The results imply that the restoring of mitochondrial function though Wnt4 gene overexpression in the PINK1 mutant transgenic Drosophila may be related to autophagy and/or apoptosis.
|
['Adenosine Triphosphatases', 'Animals', 'Animals, Genetically Modified', 'Apoptosis', 'Apoptosis Regulatory Proteins', 'Autophagy', 'Drosophila Proteins', 'Gene Expression', 'Gene Knockdown Techniques', 'Glycoproteins', 'Male', 'Membrane Potential, Mitochondrial', 'Mitochondria', 'Parkinson Disease', 'Protein-Serine-Threonine Kinases', 'Wnt Proteins']
| 30,526,176
|
[['D08.811.277.040.025'], ['B01.050'], ['B01.050.050.136', 'B05.620.136'], ['G04.146.954.035'], ['D12.644.360.075', 'D12.776.476.075'], ['G04.011'], ['D12.776.093.500.462'], ['G05.297'], ['E05.393.335.500'], ['D09.400.430', 'D12.776.395'], ['G03.295.770.500', 'G04.580.550', 'G07.265.675.550'], ['A11.284.430.214.190.875.564', 'A11.284.835.626'], ['C10.228.140.079.862.500', 'C10.228.662.600.400', 'C10.574.928.750'], ['D08.811.913.696.620.682.700'], ['D12.776.467.984', 'D23.529.984']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Radiation-dose dependence of the formation of micronuclei in misonidazole treated cell cultures.
|
The effect of misonidazole at different concentrations on the anoxic radiation sensitivity of Chinese hamster cells was investigated using the frequency of radiation induced micronuclei as criterion. The result indicates that, in a high radiation dose region, sensitization with a dose modifying factor of about 1.9 and 1.3 occurs after treatment with the substance at a concentration of 8 and 0.2 mmol/l, respectively. In a low dose region the corresponding values were 1.7 and 0.8. It was concluded that high concentration of the substance in combination with high radiation doses are most beneficial.
|
['Animals', 'Cell Division', 'Cell Line', 'Cell Nucleus', 'Cricetinae', 'Cricetulus', 'Fibroblasts', 'Misonidazole', 'Nitroimidazoles', 'Radiation Dosage']
| 6,289,614
|
[['B01.050'], ['G04.144.220', 'G04.161.750.500', 'G05.113', 'G07.345.249.410.750.500'], ['A11.251.210'], ['A11.284.430.106', 'A11.284.430.214.190.875.117'], ['B01.050.150.900.649.313.992.635.075.250'], ['B01.050.150.900.649.313.992.635.075.250.250'], ['A11.329.228'], ['D02.640.672.530', 'D03.383.129.308.658.530'], ['D02.640.672', 'D03.383.129.308.658'], ['E05.799.513', 'G01.750.740', 'N06.850.810.250']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Morphologic, hemodynamic and coronary perfusion characteristics in severe left ventricular hypertrophy secondary to systemic hypertension and evidence for nonatherosclerotic myocardial ischemia.
|
Patients with the clinical diagnosis of ischemic heart disease who were found to be free of significant coronary artery atherosclerotic disease (n = 150) underwent coronary vasodilator reserve testing, 2-dimensional echocardiography, and dipyridamole limited-stress thallium testing. After exclusions (predominantly for technically poor coronary artery Doppler signals or suboptimal echocardiography), 100 patients formed the study population. The purpose was to characterize typical cardiac and coronary artery findings in hypertensive patients with severe left ventricular (LV) hypertrophy (n = 15) and to investigate the evidence for myocardial ischemia unrelated to coronary atherosclerosis in early and advanced hypertensive heart disease. Normotensive and hypertensive control groups without LV hypertrophy (n = 12 and 34, respectively) were used for comparison. Severe LV hypertrophy was defined as LV mass index greater than or equal to 50% above established gender specific norms using 2-dimensional-directed M-mode echocardiography and the cube equation corrected to agree with necropsy estimates of mass. Clinical characteristics more often associated with severe LV hypertrophy were black race (67%), diabetes mellitus (33%), proteinuria (47%) and elevated creatinine (1.5 +/- 0.9 mg/dl). Baseline electrocardiograms and dipyridamole limited-stress thallium scans were highly likely to be abnormal (94 and 73%, respectively). Both eccentric and concentric cardiac hypertrophies were found in the severe group. Ejection fraction was significantly lower (0.51 vs 0.68, p = 0.002) and basal coronary flow velocity higher (12.0 vs 5.0 cm/s, p = 0.0004) among these patients when compared with normotensive control patients. Coronary flow reserve did not differ between control groups but was significantly depressed in patients with severe LV hypertrophy (2.5 vs 3.9, p = 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)
|
['Adult', 'Aged', 'Analysis of Variance', 'Cardiomegaly', 'Coronary Artery Disease', 'Coronary Circulation', 'Diagnosis, Differential', 'Echocardiography', 'Female', 'Humans', 'Hypertension', 'Male', 'Middle Aged', 'Thallium Radioisotopes', 'Vascular Resistance']
| 1,530,994
|
[['M01.060.116'], ['M01.060.116.100'], ['E05.318.740.150', 'N05.715.360.750.125', 'N06.850.520.830.150'], ['C14.280.195', 'C23.300.775.250'], ['C14.280.647.250.260', 'C14.907.137.126.339', 'C14.907.585.250.260'], ['G09.330.100.324'], ['E01.171'], ['E01.370.350.130.750', 'E01.370.350.850.220', 'E01.370.370.380.220'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C14.907.489'], ['M01.060.116.630'], ['D01.496.749.900'], ['G09.330.380.921']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Chemicals and Drugs [D]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Evolution of river dolphins.
|
The world's river dolphins (Inia, Pontoporia, Lipotes and Platanista) are among the least known and most endangered of all cetaceans. The four extant genera inhabit geographically disjunct river systems and exhibit highly modified morphologies, leading many cetologists to regard river dolphins as an unnatural group. Numerous arrangements have been proposed for their phylogenetic relationships to one another and to other odontocete cetaceans. These alternative views strongly affect the biogeographical and evolutionary implications raised by the important, although limited, fossil record of river dolphins. We present a hypothesis of river dolphin relationships based on phylogenetic analysis of three mitochondrial genes for 29 cetacean species, concluding that the four genera represent three separate, ancient branches in odontocete evolution. Our molecular phylogeny corresponds well with the first fossil appearances of the primary lineages of modern odontocetes. Integrating relevant events in Tertiary palaeoceanography, we develop a scenario for river dolphin evolution during the globally high sea levels of the Middle Miocene. We suggest that ancestors of the four extant river dolphin lineages colonized the shallow epicontintental seas that inundated the Amazon, Paran?, Yangtze and Indo-Gangetic river basins, subsequently remaining in these extensive waterways during their transition to freshwater with the Late Neogene trend of sea-level lowering.
|
['Animals', 'Biological Evolution', 'Cytochrome b Group', 'DNA, Mitochondrial', 'Dolphins', 'Evolution, Molecular', 'Fossils', 'Phylogeny']
| 11,296,868
|
[['B01.050'], ['G05.045', 'G16.075'], ['D08.244.187', 'D12.776.422.220.187'], ['D13.444.308.283.225'], ['B01.050.150.900.649.313.875.267'], ['G05.045.250', 'G16.075.250'], ['I01.076.368.584.311'], ['G05.697', 'G16.075.605', 'L01.100.697']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Information Science [L]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
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Effects of nifedipine on arterial concentration and myocardial extraction of catecholamines during pacing-induced angina pectoris.
|
We studied nine patients with arteriosclerotic coronary heart disease (Group I) and 10 patients with normal coronary arteries (Group II) to elucidate mechanisms by which nifedipine affects catecholamine arterial levels and myocardial extraction at rest and during pacing. Nifedipine induced signs of general and coronary vasodilatation and improved myocardial lactate metabolism during pacing. Noradrenaline arterial concentration increased significantly after nifedipine in both groups (p less than 0.01). In Group I, the values were 40-50% higher compared with Group II. The net myocardial extraction of noradrenaline was not significantly affected by nifedipine, except during pacing in Group II, in which an increase in myocardial release was seen (p less than 0.05). In Group I, there was no correlation between arterial concentration of noradrenaline and myocardial release, while there was a significant negative correlation in Group II (p less than 0.0001). Adrenaline arterial concentration and myocardial extraction were not altered by nifedipine, but there was a positive significant correlation in Group I between arterial concentration and myocardial extraction of adrenaline (p less than 0.001). In conclusion, nifedipine increased noradrenaline levels in both groups of patients, probably reflecting a rise in sympathetic activity. Because myocardial lactate production turned into extraction in patients with coronary artery disease, the increased sympathetic tone evidently did not outweigh the beneficial antianginal effects of nifedipine.
|
['Adult', 'Aged', 'Angina Pectoris', 'Arteries', 'Cardiac Pacing, Artificial', 'Catecholamines', 'Female', 'Hemodynamics', 'Humans', 'Male', 'Middle Aged', 'Myocardium', 'Nifedipine']
| 6,200,708
|
[['M01.060.116'], ['M01.060.116.100'], ['C14.280.647.187', 'C14.907.585.187', 'C23.888.592.612.233.500'], ['A07.015.114'], ['E02.331.200'], ['D02.092.311', 'D02.455.426.559.389.657.166.175'], ['G09.330.380'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['A02.633.580', 'A07.541.704', 'A10.690.552.750'], ['D03.383.725.203.540']]
|
['Named Groups [M]', 'Diseases [C]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Spirituality, dimensional autism, and schizotypal traits: The search for meaning.
|
The relationships of spirituality with human social cognition, as exemplified in autism spectrum and schizophrenia spectrum cognitive variation, remain largely unstudied. We quantified non-clinical levels of autism spectrum and schizotypal spectrum traits (using the Autism Quotient and the Schizotypal Personality Questionnaire-Brief Revised) and dimensions of spirituality (using the Hardt Spirituality Questionnaire) in a large sample of undergraduate students. We tested in particular the hypothesis, based on the diametrical model of autism and psychosis, that autism should be negatively associated, and positive schizotypal traits should be positively associated, with spirituality. Our primary findings were threefold. First, in support of the diametric model, total Spirituality score was significantly negatively correlated with total Autism Quotient score, and significantly positively correlated with Positive Schizotypal traits (the Schizotypal Personality Cognitive-Perceptual subscale), as predicted. Second, these associations were driven mainly by opposite patterns regarding the Search for Meaning Spirituality subscale, which was the only subscale that was significantly negatively associated with autism, and significantly positively associated with Positive Schizotypal traits. Third, Belief in God was positively correlated with Positive Schizotypal traits, but was uncorrelated with autism traits. The opposite findings for Search for Meaning can be interpreted in the contexts of well-supported cognitive models for understanding autism in terms of weak central coherence, and understanding Positive Schizotypal traits in terms of enhanced salience.
|
['Alberta', 'Autism Spectrum Disorder', 'Autistic Disorder', 'Female', 'Humans', 'Male', 'Models, Psychological', 'Multivariate Analysis', 'Schizotypal Personality Disorder', 'Spirituality', 'Surveys and Questionnaires', 'Young Adult']
| 30,849,096
|
[['Z01.107.567.176.064'], ['F03.625.164.113'], ['F03.625.164.113.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.599.695'], ['E05.318.740.150.500', 'N05.715.360.750.125.500', 'N06.850.520.830.150.500'], ['F03.675.725'], ['F02.880.705', 'K01.844.664.500'], ['E05.318.308.980', 'N05.715.360.300.800', 'N06.850.520.308.980'], ['M01.060.116.815']]
|
['Geographicals [Z]', 'Psychiatry and Psychology [F]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Humanities [K]', 'Named Groups [M]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 1
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Molecular crowding reduces to a similar extent the diffusion of small solutes and macromolecules: measurement by fluorescence correlation spectroscopy.
|
Aqueous environments in living cells are crowded, with up to >50 wt% small and macromolecule-size solutes. We investigated quantitatively one important consequence of molecular crowding--reduced diffusion of biologically important solutes. Fluorescence correlation spectroscopy (FCS) was used to measure the diffusion of a series of fluorescent small solutes and macromolecules. In water, diffusion coefficients (D(o)w) were (in cm2/s x 10(-8)): rhodamine green (270), albumin (52), dextrans (75, 10 kDa; 10, 500 kDa), double-stranded DNAs (96, 20 bp; 10, 1 kb; 3.4, 4.5 kb) and polystyrene nanospheres (5.4, 20 nm diameter; 2.3, 100 nm). Aqueous-phase diffusion (Dw) in solutions crowded with Ficoll-70 (0-60 wt%) was reduced by up to 650-fold in an exponential manner: Dw = D(o)w exp (-[C]/[C]exp), where [C]exp is the concentration (in wt%) of crowding agent reducing D(o)w by 63%. FCS data for all solutes and Ficoll-70 concentrations fitted well to a model of single-component, simple (non-anomalous) diffusion. Interestingly [C]exp were nearly identical (11+/-2 wt%, SD) for diffusion of the very different types of macromolecules in Ficoll-70 solutions. However, [C]exp was dependent on the nature of the crowding agent: for example, [C]exp for diffusion of rhodamine green was 30 wt% for glycerol and 16 wt% for 500 kDa dextran. Our results indicate that molecular crowding can greatly reduce aqueous-phase diffusion of biologically important macromolecules, and demonstrate a previously unrecognized insensitivity of crowding effects on the size and characteristics of the diffusing species.
|
['Diffusion', 'Ficoll', 'Macromolecular Substances', 'Solutions', 'Spectrometry, Fluorescence', 'Water']
| 15,362,103
|
[['G01.202', 'G02.196'], ['D09.698.330'], ['D05'], ['D26.776'], ['E05.196.712.516.600.676', 'E05.196.867.726'], ['D01.045.250.875', 'D01.248.497.158.459.650', 'D01.650.550.925']]
|
['Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Expanding indications for valve-sparing aortic root reconstruction: early and midterm results.
|
BACKGROUND: Valve-sparing aortic root reconstruction (VSRR) is an accepted method to treat patients with aortic root dilation. The role of the VSRR is less well defined for patients with bicuspid aortic valve, severe aortic valve insufficiency, congenital heart defects, and type A aortic dissection. We studied the clinical outcome of patients who underwent VSRR for expanded indications.METHODS: Seventy-eight patients underwent VSRR between the 2005 and 2012. Seventy-two patients (92%) underwent reimplantation and 6 patients (8%) were operated on with the remodeling technique. The mean age was 51 ± 12 years (range 24 to 73). For 71 patients (91%), the operation was elective, and for 7 (9%; all with type A aortic dissection), on an emergency basis. Preoperatively, the degree of aortic insufficiency was graded as 2+ or greater for 27 patients (35%). Connective tissue disorder (Marfan or Loeys-Dietz), bicuspid aortic valve, or congenital heart disease was present in 15 (19%), 15 (19%), and 7 patients (9%), respectively. Concomitant aortic valve leaflet repair was performed for 39 patients (50%). The mean follow-up time was 2.4 ± 1.7 years (range, 0.1 to 7.0).RESULTS: Thirty-day mortality was zero. The rate of postoperative complications was low: stroke 3%, renal failure 3%, prosthesis infection 1%, and low cardiac output syndrome 1%. Survival was 100% at 1 year and 97% at 5 years. Freedom from recurrent aortic valve insufficiency (?2+) during the follow-up was 94%.CONCLUSIONS: The midterm results of VSRR in terms of survival, freedom from recurrent aortic valve insufficiency, and the need for reoperation are excellent, even for high-risk patients.
|
['Adult', 'Aged', 'Aortic Aneurysm, Thoracic', 'Cardiac Surgical Procedures', 'Feasibility Studies', 'Female', 'Humans', 'Male', 'Middle Aged', 'Prospective Studies', 'Time Factors', 'Treatment Outcome', 'Vascular Surgical Procedures', 'Young Adult']
| 23,103,004
|
[['M01.060.116'], ['M01.060.116.100'], ['C14.907.055.239.125', 'C14.907.109.139.125'], ['E04.100.376', 'E04.928.220'], ['E05.318.372.550', 'E05.337.675', 'N05.715.360.330.550', 'N06.850.520.450.550'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['G01.910.857'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['E04.100.814'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
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The attractions of medicine: the generic motivations of medical school applicants in relation to demography, personality and achievement.
|
BACKGROUND: The motivational and other factors used by medical students in making their career choices for specific medical specialities have been looked at in a number of studies in the literature. There are however few studies that assess the generic factors which make medicine itself of interest to medical students and to potential medical students. This study describes a novel questionnaire that assesses the interests and attractions of different aspects of medical practice in a varied range of medical scenarios, and relates them to demographic, academic, personality and learning style measures in a large group of individuals considering applying to medical school.METHODS: A questionnaire study was conducted among those attending Medlink, a two-day conference for individuals considering applying to medical school for a career in medicine. The main outcome measure was the Medical Situations Questionnaire, in which individuals ranked the attraction of three different aspects of medical practise in each of nine detailed, realistic medical scenarios in a wide range of medical specialities. As well as requiring clear choices, the questionnaire was also designed so that all of the possible answers were attractive and positive, thereby helping to eliminate social demand characteristics. Factor analysis of the responses found four generic motivational dimensions, which we labelled Indispensability, Helping People, Respect and Science. Background factors assessed included sex, ethnicity, class, medical parents, GCSE academic achievement, the 'Big Five' personality factors, empathy, learning styles, and a social desirability scale.RESULTS: 2867 individuals, broadly representative of applicants to medical schools, completed the questionnaire. The four generic motivational factors correlated with a range of background factors. These correlations were explored by multiple regression, and by path analysis, using LISREL to assess direct and indirect effects upon the factors. Helping People was particularly related to agreeableness; Indispensability to a strategic approach to learning; Respect to a surface approach to learning; and Science to openness to experience. Sex had many indirect influences upon generic motivations. Ethnic origin also had indirect influences via neuroticism and surface learning, and social class only had indirect influences via lower academic achievement. Coming from a medical family had no influence upon generic motivations.CONCLUSION: Generic motivations for medicine as a career can be assessed using the Medical Situations Questionnaire, without undue response bias due to demand characteristics. The validity of the motivational factors is suggested by the meaningful and interpretable correlations with background factors such as demographics, personality, and learning styles. Further development of the questionnaire is needed if it is to be used at an individual level, either for counselling or for student selection.
|
['Adolescent', 'Adult', 'Career Choice', 'Congresses as Topic', 'Demography', 'Education, Medical', 'Educational Status', 'Empathy', 'Ethnic Groups', 'Factor Analysis, Statistical', 'Female', 'Humans', 'Learning', 'Male', 'Motivation', 'Personality', 'Schools, Medical', 'Sex Factors', 'Social Desirability', 'Specialization', 'Students', 'Surveys and Questionnaires', 'United Kingdom']
| 16,504,048
|
[['M01.060.057'], ['M01.060.116'], ['F02.463.785.373.346.400'], ['N03.540.199'], ['I01.240', 'N01.224', 'N06.850.505.400'], ['I02.358.399'], ['N01.824.196'], ['F01.752.355', 'F01.752.543.500.500'], ['M01.686.754', 'N01.224.317'], ['E05.318.740.400', 'N05.715.360.750.350', 'N06.850.520.830.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F02.463.425', 'F02.784.629.529'], ['F01.658', 'F01.752.543.500.750'], ['F01.752'], ['I02.783.495.552', 'N02.278.020.578'], ['N05.715.350.675', 'N06.850.490.875'], ['F01.145.813.628'], ['H02.811'], ['M01.848'], ['E05.318.308.980', 'N05.715.360.300.800', 'N06.850.520.308.980'], ['Z01.542.363']]
|
['Named Groups [M]', 'Psychiatry and Psychology [F]', 'Health Care [N]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Disciplines and Occupations [H]', 'Geographicals [Z]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 1
| 1
|
Development of homologous radioimmunoassays for equine growth hormone and equine prolactin and their application to the detection of circulating levels of hormone in horse plasma.
|
Highly purified and well-characterised preparations of equine prolactin and growth hormone from equine pituitary glands were employed to set up highly sensitive and specific homologous radioimmunoassays (RIA) for the measurement of hormone in horse plasma. The limit of sensitivity of the GH RIA was 1.2 ng/ml with mean intra- and inter-assay coefficients of variation (CV) of 6.6 and 10%, respectively. The sensitivity of the equine prolactin (ePRL) RIA was 0.5 ng/ml with mean intra and inter-assay CV of 9.1 and 15.6%, respectively. Dose-response curves of a crude pituitary gland extract and plasma samples collected from a mare and foal were parallel to the standards and the PRL RIA was clinically validated by administration of thyrotropin-releasing hormone (TRH). Plasma samples taken at 15 min intervals over 24 h from lactating mares gave 24 h mean GH values in the range 5.5 to 7.95 ng/ml. Large intermittent elevations of GH activity were detected. The mean 24 h PRL concentrations were between 3.2-10.4 ng/ml in the lactating animals, with higher concentrations earlier in lactation. Long episodic bursts of PRL were detected.
|
['Animals', 'Biological Assay', 'Columbidae', 'Dwarfism, Pituitary', 'Fasting', 'Female', 'Growth Hormone', 'Growth Hormone-Releasing Hormone', 'Guinea Pigs', 'Horses', 'Lactation', 'Mammary Glands, Animal', 'Mice', 'Mice, Mutant Strains', 'Peptide Fragments', 'Pituitary Gland, Anterior', 'Prolactin', 'Rabbits', 'Radioimmunoassay', 'Reference Standards', 'Reproducibility of Results', 'Secretory Rate', 'Sensitivity and Specificity', 'Stomach, Avian', 'Thyrotropin-Releasing Hormone']
| 7,986,349
|
[['B01.050'], ['E05.091'], ['B01.050.150.900.248.165.150'], ['C05.116.099.343.445', 'C05.116.132.358', 'C10.228.140.617.738.300.300', 'C19.297.312', 'C19.700.482.311'], ['F01.145.407.400', 'G07.203.650.240.587', 'G07.203.650.353.400'], ['D06.472.699.631.525.425', 'D12.644.548.691.525.425'], ['D06.472.699.327.740.860', 'D12.644.400.400.740.860', 'D12.644.548.365.740.860', 'D12.776.631.650.405.740.860'], ['B01.050.150.900.649.313.992.550'], ['B01.050.150.900.649.313.984.235.472'], ['G08.686.523', 'G08.686.702.500'], ['A10.336.482', 'A13.589'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.150.900.649.313.992.635.505.500.550'], ['D12.644.541'], ['A06.300.747.500', 'A06.688.357.750.500', 'A08.186.211.180.497.352.435.500.500', 'A08.186.211.200.317.357.352.435.500.500', 'A08.713.357.750.500'], ['D06.472.699.322.576.773', 'D06.472.699.631.525.525', 'D12.644.548.691.525.525'], ['B01.050.150.900.649.313.968.700'], ['E01.370.384.700', 'E05.478.566.639', 'E05.601.470.639'], ['E05.978.808'], ['E05.318.370.725', 'E05.337.851', 'N05.715.360.325.685', 'N06.850.520.445.725'], ['G03.857'], ['E05.318.370.800', 'E05.318.740.872', 'G17.800', 'N05.715.360.325.700', 'N05.715.360.750.725', 'N06.850.520.445.800', 'N06.850.520.830.872'], ['A13.853'], ['D06.472.699.327.740.880', 'D12.644.400.400.740.880', 'D12.644.456.837', 'D12.644.548.365.740.880', 'D12.776.631.650.405.740.880', 'D12.776.631.650.810']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Health Care [N]']
| 1
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
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The impact of prospective pricing on the information system in the health care industry.
|
The move from a retrospective payment system (value added) to a prospective payment system (diagnostic related) has not only influenced the health care business but also changed their information systems' requirements. The change in requirements can be attributed both to an increase in data processing tasks and also to an increase in the need for information to more effectively manage the organization. A survey was administered to capture the response of health care institutions, in the area of information systems, to the prospective payment system. The survey results indicate that the majority of health care institutions have responded by increasing their information resources, both in terms of hardware and software, and have moved to integrate the medical and financial data. In addition, the role of the information system has changed from a cost accounting system to one intended to provide a competitive edge in a highly competitive marketing environment.
|
['Data Collection', 'Hospital Administration', 'Hospital Bed Capacity', 'Hospital Information Systems', 'Ownership', 'Prospective Payment System', 'Statistics as Topic', 'United States']
| 3,397,683
|
[['E05.318.308', 'L01.399.250', 'N05.715.360.300', 'N06.850.520.308'], ['H02.309', 'N02.278.216.500', 'N04.452.442.452'], ['N02.278.306.472'], ['N04.452.442.452.452', 'N04.452.515.360'], ['I01.880.604.583.594', 'N04.452.633'], ['N03.219.521.710.305.200'], ['E05.318.740', 'H01.548.832', 'N05.715.360.750', 'N06.850.520.830'], ['Z01.107.567.875']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Information Science [L]', 'Health Care [N]', 'Disciplines and Occupations [H]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Geographicals [Z]']
| 0
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 1
|
Transcutaneous meniscectomy under arthroscopic control.
|
A technique of arthroscopic excision of a torn meniscus has evolved during two years' experience of therapeutic arthroscopy of the knee joint. The same method is used for both medial and lateral meniscus lesions under full visual control throughout the procedure, with a standard arthroscope inserted centrally through the patellar tendon. The first 18 consecutive patients thus treated have been followed up. Endoscopic operation was complemented by arthrotomy in three; in 15 no other treatment was given. The time in hospital, convalescence, sick leave, and knee function at follow-up were compared in these 15 patients with matched controls treated in the ordinary way by arthrotomy. Knee function did not differ between the groups, whereas all other variables showed better results after arthroscopic excision.
|
['Endoscopes', 'Endoscopy', 'Humans', 'Knee Injuries', 'Length of Stay']
| 549,862
|
[]
|
[]
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
A comparison of the interfacial interactions of the apoprotein from high density lipoprotein and beta-casein with phospholipids.
|
The conformations adopted by beta-casein and the total apoprotein from serum high density lipoprotein when spread at the air-water interface are compared; the monolayer data are consistent with the apoprotein being alpha-helical and the beta-casein being disordered with segments distributed in loops and trains. The penetration of these hydrophobic proteins into phosphatidylcholine monolayers in different physical states was investigated. More protein can penetrate into monolayers when they are in the liquid-expanded state; for penetration at constant total surface area the lateral compressibility of the lipid is an important factor. The charge and conformation of the polar group of the phospholipid does not have a major influence on the interaction. The mixed films of lipid and protein have a mosaic structure; probably the beta-casein is in a compressed state whereas the apoprotein is extended as alpha-helices in the plane of the interface. The chain-length depedences of the interaction of the apoprotein with phosphatidylcholine monolayers and bilayers are different; when the apoprotein binds to bilayers of shorter-chain phosphatidylcholines it alters the shape of the lipid-water interface whereas with monolayers the interface remains planar throughout.
|
['Apoproteins', 'Binding Sites', 'Caseins', 'Humans', 'Lipoproteins, HDL', 'Membranes, Artificial', 'Models, Biological', 'Phosphatidylcholines', 'Phosphatidylethanolamines', 'Phosphatidylserines', 'Phospholipids', 'Pressure', 'Protein Binding', 'Protein Conformation', 'Surface Properties']
| 170,986
|
[['D12.776.070'], ['G02.111.570.120'], ['D12.776.256.159.750.207', 'D12.776.744.150'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D10.532.432', 'D12.776.521.479'], ['D25.479', 'J01.637.051.479', 'J01.637.087.500'], ['E05.599.395'], ['D10.570.755.375.760.400.800'], ['D10.570.755.375.760.400.840'], ['D10.570.755.375.760.400.971'], ['D10.570.755'], ['G01.374.715'], ['G02.111.679', 'G03.808'], ['G02.111.570.820.709'], ['G02.860']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Technology, Industry, and Agriculture [J]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Catalysis of slow C-terminal processing reactions by carboxypeptidase H.
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A hypothesis was examined that carboxypeptidase H (CpAse H), which is known to catalyse the release of lysine and arginine from the C-terminus of peptides, can also release histidine, tyrosine, and phenylalanine. Synthetic peptides terminating in -His-Lys or -Tyr-Lys were used as model substrates for the enzyme and amino acid analysis was employed to detect release of the terminal amino acids. With N-acetyl-beta-Ala-Asn-Ala-His-Lys and N-acetyl-beta-Ala-Asn-Ala-Tyr-Lys, which correspond to intermediates in the processing of porcine and human beta-endorphin, lysine was removed rapidly and quantitatively but no release of histidine or tyrosine could be detected. To allow more sensitive analysis, radiolabelled substrates were employed and the amounts of the products formed on incubation with CpAse H were determined after separation by ion-exchange chromatography. With 125I-D-Tyr-Ala-His-Lys-Lys as substrate at pH 5.7, very small amounts of D-Tyr-Ala were released; the main product was D-Tyr-Ala-His. At pH 5.0 the release of histidine from 125I-D-Tyr-Ala-His took place 6,000 times more slowly than the release of lysine from 125I-D-Tyr-Ala-Lys. When the tripeptides were incubated at pH 5 with porcine pituitary secretory granules, the lysine was released rapidly but no release of histidine could be detected. The results demonstrate that CpAse H catalyses the release of C-terminal histidine with great difficulty.(ABSTRACT TRUNCATED AT 250 WORDS)
|
['Amino Acid Sequence', 'Animals', 'Arginine', 'Carboxypeptidase H', 'Carboxypeptidases', 'Catalysis', 'Chemical Phenomena', 'Chemistry', 'Histidine', 'Humans', 'Lysine', 'Peptides', 'Swine', 'Tyrosine', 'beta-Endorphin']
| 2,526,198
|
[['G02.111.570.060', 'L01.453.245.667.060'], ['B01.050'], ['D12.125.068.050', 'D12.125.095.104', 'D12.125.142.087'], ['D08.811.277.656.350.245.167', 'D08.811.277.656.350.555.250', 'D08.811.277.656.675.555.250', 'D08.811.277.656.837.124'], ['D08.811.277.656.350.245'], ['G02.130'], ['G02'], ['H01.181'], ['D12.125.072.329', 'D12.125.142.308'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.125.068.555', 'D12.125.095.647', 'D12.125.142.497'], ['D12.644'], ['B01.050.150.900.649.313.500.880'], ['D12.125.072.050.875'], ['D06.472.699.327.935.239', 'D06.472.699.631.525.600.239', 'D12.644.400.400.935.239', 'D12.644.400.575.241.080', 'D12.644.548.365.935.239', 'D12.644.548.691.525.690.239', 'D12.776.631.650.405.935.239', 'D12.776.631.650.575.241.080']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Disciplines and Occupations [H]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
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A mutation in synaptojanin 2 causes progressive hearing loss in the ENU-mutagenised mouse strain Mozart.
|
BACKGROUND: Hearing impairment is the most common sensory impairment in humans, affecting 1:1,000 births. We have identified an ENU generated mouse mutant, Mozart, with recessively inherited, non-syndromic progressive hearing loss caused by a mutation in the synaptojanin 2 (Synj2), a central regulatory enzyme in the phosphoinositide-signaling cascade.METHODOLOGY/PRINCIPAL FINDINGS: The hearing loss in Mozart is caused by a p.Asn538Lys mutation in the catalytic domain of the inositol polyphosphate 5-phosphatase synaptojanin 2. Within the cochlea, Synj2 mRNA expression was detected in the inner and outer hair cells but not in the spiral ganglion. Synj2(N538K) mutant protein showed loss of lipid phosphatase activity, and was unable to degrade phosphoinositide signaling molecules. Mutant Mozart mice (Synj2(N538K/N538K)) exhibited progressive hearing loss and showed signs of hair cell degeneration as early as two weeks of age, with fusion of stereocilia followed by complete loss of hair bundles and ultimately loss of hair cells. No changes in vestibular or neurological function, or other clinical or behavioral manifestations were apparent.CONCLUSIONS/SIGNIFICANCE: Phosphoinositides are membrane associated signaling molecules that regulate many cellular processes including cell death, proliferation, actin polymerization and ion channel activity. These results reveal Synj2 as a critical regulator of hair cell survival that is essential for hair cell maintenance and hearing function.
|
['Amino Acid Sequence', 'Amino Acid Substitution', 'Animals', 'Behavior, Animal', 'Ethylnitrosourea', 'Hair Cells, Auditory', 'Hearing Loss, Sensorineural', 'Inositol Polyphosphate 5-Phosphatases', 'Male', 'Mice', 'Mice, Inbred C57BL', 'Mice, Mutant Strains', 'Molecular Sequence Data', 'Mutagenesis', 'Mutation', 'Nerve Fibers', 'Nerve Tissue Proteins', 'Neural Conduction', 'Phosphoric Monoester Hydrolases', 'Spiral Ganglion', 'Vestibule, Labyrinth']
| 21,423,608
|
[['G02.111.570.060', 'L01.453.245.667.060'], ['E05.393.420.601.035', 'G05.558.109'], ['B01.050'], ['F01.145.113'], ['D02.065.950.594.310', 'D02.654.692.300'], ['A08.675.650.250', 'A08.675.650.915.750.600.350', 'A08.800.950.750.600.350', 'A09.246.300.246.577.325', 'A11.671.650.250', 'A11.671.650.915.750.600.350'], ['C09.218.458.341.887', 'C10.597.751.418.341.887', 'C23.888.592.763.393.341.887'], ['D08.811.277.352.650.624.500'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.420', 'B01.050.150.900.649.313.992.635.505.500.400.420'], ['B01.050.150.900.649.313.992.635.505.500.550'], ['L01.453.245.667'], ['G05.558'], ['G05.365.590'], ['A08.675.542', 'A11.671.501'], ['D12.776.631'], ['G07.265.753', 'G11.561.601'], ['D08.811.277.352.650'], ['A08.340.390.800', 'A08.800.350.800', 'A08.800.800.120.910.120.800', 'A09.246.300.246.900'], ['A09.246.300.909']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Psychiatry and Psychology [F]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Early limb salvage: open tibia fractures of Ambroise Par? (1510-1590) and Percivall Pott (1714-1789).
|
Ambroise Par? and Percivall Pott were preeminent clinicians, writers, and educators who advanced the cause of surgery through their independent thinking and insightful observations. In addition to their legacies and numerous enduring contributions, both men sustained open tibial fractures that were successfully managed during times when most often amputation was prescribed for such injuries. This article explores their lives and their fractures.
|
['Ankle Injuries', 'England', 'Eponyms', 'Fracture Fixation', 'Fractures, Open', 'General Surgery', 'History, 16th Century', 'History, 18th Century', 'Humans', 'Male', 'Tibial Fractures']
| 8,943,188
|
[['C26.558.100'], ['Z01.542.363.300'], ['L01.559.598.400.556.536'], ['E04.555.300'], ['C26.404.311'], ['H02.403.810.300'], ['K01.400.475.750'], ['K01.400.504.875'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C26.404.875', 'C26.558.857']]
|
['Diseases [C]', 'Geographicals [Z]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Humanities [K]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
|
Diabetic retinopathy, duration of diabetes and risk factors of atherosclerotic cardiovascular disease.
|
The present study, concerning 145 insulin-dependent diabetics showed positive relationships between the severity of retinal disease on the one hand, and body weight, blood pressure, and serum cholesterol level on the other. These relationships remain significant when the duration of the clinical diabetes and the age of the patient are taken into account. Two interpretations are suggested. They are not incompatible. In diabetic subjects, either the increase in blood pressure and serum cholesterol level causes an aggravation of diabetic retinopathy or there exists a common factor at the origin of retinal lesions and of an increase in risk of cardiovascular disease through atherosclerosis.
|
['Adult', 'Age Factors', 'Blood Pressure', 'Body Height', 'Body Weight', 'Cholesterol', 'Coronary Disease', 'Diabetes Complications', 'Diabetic Retinopathy', 'Humans', 'Insulin', 'Lipids', 'Risk', 'Time Factors']
| 1,229,803
|
[['M01.060.116'], ['N05.715.350.075', 'N06.850.490.250'], ['E01.370.600.875.249', 'G09.330.380.076'], ['E01.370.600.115.100.160.100', 'E05.041.124.160.500', 'G07.100.100.160.100', 'G07.345.249.314.100'], ['C23.888.144', 'E01.370.600.115.100.160.120', 'E05.041.124.160.750', 'G07.100.100.160.120', 'G07.345.249.314.120'], ['D04.210.500.247.222.284', 'D04.210.500.247.808.197', 'D10.570.938.208'], ['C14.280.647.250', 'C14.907.585.250'], ['C19.246.099'], ['C11.768.257', 'C14.907.320.382', 'C19.246.099.500.382'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D06.472.699.587.200.500.625', 'D12.644.548.586.200.500.625'], ['D10'], ['E05.318.740.600.800', 'G17.680.750', 'N05.715.360.750.625.700', 'N06.850.520.830.600.800'], ['G01.910.857']]
|
['Named Groups [M]', 'Health Care [N]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Morphological Versatility in the Self-Assembly of Val-Ala and Ala-Val Dipeptides.
|
Since the discovery of dipeptide self-assembly, diphenylalanine (Phe-Phe)-based dipeptides have been widely investigated in a variety of fields. Although various supramolecular Phe-Phe-based structures including tubes, vesicles, fibrils, sheets, necklaces, flakes, ribbons, and wires have been demonstrated by manipulating the external physical or chemical conditions applied, studies of the morphological diversity of dipeptides other than Phe-Phe are still required to understand both how these small molecules respond to external conditions such as the type of solvent and how the peptide sequence affects self-assembly and the corresponding molecular structures. In this work, we investigated the self-assembly of valine-alanine (Val-Ala) and alanine-valine (Ala-Val) dipeptides by varying the solvent medium. It was observed that Val-Ala dipeptide molecules may generate unique self-assembly-based morphologies in response to the solvent medium used. Interestingly, when Ala-Val dipeptides were utilized as a peptide source instead of Val-Ala, we observed distinct differences in the final dipeptide structures. We believe that such manipulation may not only provide us with a better understanding of the fundamentals of the dipeptide self-assembly process but also may enable us to generate novel peptide-based materials for various applications.
|
['2-Propanol', 'Dipeptides', 'Models, Molecular', 'Protein Conformation', 'Pyridines']
| 26,086,903
|
[['D02.033.755.615'], ['D12.644.456.345'], ['E05.599.595'], ['G02.111.570.820.709'], ['D03.383.725']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Regioselectivity of pyridine deprotonation in the gas phase.
|
The regioselective deprotonation of pyridine in the gas phase has been investigated by using chemical reactivity studies. The mixture of regioisomers, trapped as carboxylates, formed in an equilibrium mixture is determined to result from 70-80% deprotonation in the 4-position, and 20-30% deprotonation at the 3-position. The ion formed by deprotonation in the 2-position is not measurably deprotonated at equilibrium because the ion is destabilized by lone-pair repulsion. From the composition of the mixture, the gas-phase acidities (DeltaH degrees acid) at the 4-, 3-, and 2-positions are determined to be 389.9 +/- 2.0, 391.2-391.5, and >391.5 kcal/mol, respectively. The relative acidities of the 4- and 3-positions are explained by using Hammett-Taft parameters, derived by using the measured gas-phase acidities of pyridine carboxylic acids. The values of sigmaF and sigmaR are -0.18 and 0.74, respectively, showing the infused nitrogen in pyridine to have a strong pi electron-withdrawing effect, but with little sigma-inductive effect.
|
['Carboxylic Acids', 'Hydrocarbons, Aromatic', 'Kinetics', 'Nitro Compounds', 'Nitrogen', 'Protons', 'Pyridines']
| 17,266,374
|
[['D02.241'], ['D02.455.426.559'], ['G01.374.661', 'G02.111.490'], ['D02.640'], ['D01.268.604', 'D01.362.625'], ['D01.248.497.300.459.700', 'D01.268.406.750', 'D01.362.340.750', 'G01.249.660.500'], ['D03.383.725']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]']
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Appliance selection. Treatment objectives and esthetic concerns.
|
The adaptability of modern orthodontic appliances to meet the esthetic needs of the adult patient while satisfying the treatment goals agreed upon has been shown. Some of the treatments cited could be accomplished by the general practitioner, while other modalities should be handled by the orthodontist. The outstanding advances in orthognathic surgical technique provide the patient with additional options for orthodontic corrections of malocclusions while reducing the time needed to wear braces. Proper planning and interaction among the dental disciplines can greatly enhance a patient's final outcome. It behooves all clinicians to look at all the options available in treating the adult so these patients can benefit most from our services.
|
['Adult', 'Esthetics, Dental', 'Female', 'Humans', 'Male', 'Malocclusion', 'Middle Aged', 'Orthodontic Appliances', 'Orthodontics, Corrective', 'Patient Compliance', 'Space Maintenance, Orthodontic']
| 3,165,352
|
[['M01.060.116'], ['E06.420'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C07.793.494'], ['M01.060.116.630'], ['E06.658.453'], ['E06.658.578'], ['F01.100.150.750.500.600', 'F01.145.488.887.500.600', 'N05.300.150.800.500.600'], ['E06.658.703.675']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Health Care [N]']
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Distribution of type-VII collagen in xenografted human carcinomas.
|
The distribution of type-VII collagen, the main molecular component of the anchoring fibrils (AF) attaching the basal lamina (BL, lamina densa of the basement membrane) to the surrounding connective tissue, was investigated in four xenografted human carcinomas of the hypopharynx (H-Stg 1), the lung (L 261), the sigmoid colon (CA 1), and the rectum (R 85). The studies were performed with a recently prepared, affinity-purified and highly specific antibody to type-VII collagen by using the indirect immunofluorescence and the APAAP (alkaline phosphatase anti-alkaline phosphatase) techniques. For comparison, the localization of the intrinsic BL components laminin and type-IV collagen were additionally analyzed in all four carcinomas. It was shown that type-VII collagen usually colocalized to laminin and type-IV collagen and was deposited at the borderline between carcinoma cell clusters and the surrounding strands of connective tissue in a similar, but more diffuse and less continuous distribution than both intrinsic BL components. In the squamous cell carcinoma H-Stg 1 and the adenocarcinoma L261, type-VII collagen was additionally accumulated in enlarged extracellular spaces between carcinoma cells, away from the contact zone to the connective tissue and again colocalized to laminin and type-IV collagen. Numerous carcinoma cells of both xenografts showed remarkable intracytoplasmic immunoreactivity for the antibody to type-VII collagen. Even in the case of the gastrointestinal carcinomas CA 1 and R 85, faint immunoreactivity for type-VII collagen was found at the contact zone between the mucosal epithelium and the surrounding connective tissue. These results confirm that epithelial carcinoma cells are obviously involved with the synthesis of the main molecular component of AF usually attaching the BL to the adjacent connective tissue and hint at a possible correlation between the localization of type-VII collagen and the observed pattern of the BL. However, it cannot be decided whether there is a direct causal relation between both phenomena or whether they are both the consequence of an independent but common cause, such as abnormal cellular differentiation of carcinoma cells. In no case, can the discontinuities in the distribution of type-VII collagen be explained by active tumor cell invasion since xenografted human carcinomas neither invade nor metastasize.
|
['Adenocarcinoma', 'Animals', 'Basement Membrane', 'Carcinoma, Squamous Cell', 'Collagen', 'Colonic Neoplasms', 'Connective Tissue', 'Fluorescent Antibody Technique', 'Gastrointestinal Neoplasms', 'Humans', 'Immunohistochemistry', 'Laminin', 'Lung Neoplasms', 'Mice', 'Mice, Nude', 'Neoplasm Transplantation', 'Pharyngeal Neoplasms', 'Rectal Neoplasms', 'Transplantation, Heterologous']
| 8,339,316
|
[['C04.557.470.200.025'], ['B01.050'], ['A10.272.220', 'A10.615.179'], ['C04.557.470.200.400', 'C04.557.470.700.400'], ['D05.750.078.280', 'D12.776.860.300.250'], ['C04.588.274.476.411.307.180', 'C06.301.371.411.307.180', 'C06.405.249.411.307.180', 'C06.405.469.158.356.180', 'C06.405.469.491.307.180'], ['A10.165'], ['E01.370.225.500.607.512.240', 'E01.370.225.750.551.512.240', 'E05.200.500.607.512.240', 'E05.200.750.551.512.240', 'E05.478.583.375'], ['C04.588.274.476', 'C06.301.371', 'C06.405.249'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.225.500.607.512', 'E01.370.225.750.551.512', 'E05.200.500.607.512', 'E05.200.750.551.512', 'E05.478.583', 'H01.158.100.656.234.512', 'H01.158.201.344.512', 'H01.158.201.486.512', 'H01.181.122.573.512', 'H01.181.122.605.512'], ['D12.776.395.550.530', 'D12.776.543.550.500', 'D12.776.860.300.675'], ['C04.588.894.797.520', 'C08.381.540', 'C08.785.520'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.150.900.649.313.992.635.505.500.550.500'], ['E05.624'], ['C04.588.443.665.710', 'C07.550.745', 'C09.647.710', 'C09.775.549'], ['C04.588.274.476.411.307.790', 'C06.301.371.411.307.790', 'C06.405.249.411.307.790', 'C06.405.469.491.307.790', 'C06.405.469.860.180.500'], ['E04.936.764']]
|
['Diseases [C]', 'Organisms [B]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
Specific effects of alpha-D,L-aminoadipic acid on synaptic transmission in frog spinal cord.
|
The sucrose gap technique was employed to investigate both synaptic and amino acid evoked responses from motoneurones or primary afferents of frog spinal cord. alpha-D,L-Aminoadipic acid (alpha-D,L-AAD) selectively antagonized responses to acidic amino acids, especially aspartate. The drug was most effective in antagonizing the polysynaptic components of synaptic potentials evoked by dorsal root or lateral column stimulation but had little effect on their monosynaptic components. The ventral root dorsal root potential which is thought to be mediated by a pathway that does not involve acidic amino acids was insensitive to alpha-D,L-AAD. These data, which were confirmed by intracellular recording from motoneurones, provided further evidence for the role of acidic amino acids in polysynaptic pathways in frog spinal cord.
|
['2-Aminoadipic Acid', 'Amino Acids', 'Amino Acids, Dicarboxylic', 'Animals', 'Drug Interactions', 'Evoked Potentials', 'In Vitro Techniques', 'Motor Neurons', 'Rana pipiens', 'Spinal Cord', 'Synapses', 'Synaptic Transmission']
| 6,253,038
|
[['D02.241.081.337.052.075', 'D12.125.119.075'], ['D12.125'], ['D12.125.119'], ['B01.050'], ['G07.690.773.968'], ['G07.265.216.500', 'G11.561.200.500'], ['E05.481'], ['A08.675.655.500', 'A11.671.655.500'], ['B01.050.150.900.090.180.708.310'], ['A08.186.854'], ['A08.850', 'A11.284.149.165.420.780'], ['G02.111.820.850', 'G04.835.850', 'G07.265.880', 'G11.561.830']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Phosphorylation in hydrogen bacteria.
|
The electron-transport system of cell-free extracts obtained from Hydrogenomonas H-20 has been studied with particular reference to phosphorylation associated with the oxyhydrogen reaction. Cell-free preparations of this organism exhibit oxidative phosphorylation with hydrogen and succinate as electron donors. This activity could be uncoupled with a number of agents. Ratios of phosphorylative activity to oxidative activity observed varied from 0.2 to 0.7. Factors affecting the efficiency of phosphorylation were examined. Inhibitor and spectrophotometric studies indicated that phosphorylation with hydrogen as electron donor occurs exclusively at a site in an abbreviated electron transport chain between H(2) and cytochrome b. The possible occurrence of a cytochrome b oxidase and the requirement for a quinone are discussed, as well as the correlation between the abbreviated pathway and the energy generation by the cell. Evidence is presented which indicates that nicotinamide adenine dinucleotide does not participate in the hydrogen oxidation path which is coupled to adenosine triphosphate formation.
|
['Antimycin A', 'Cyanides', 'Cytochromes', 'Dicumarol', 'Dinitrophenols', 'Electron Transport', 'Hydrogen', 'NAD', 'Oxidative Phosphorylation', 'Pseudomonas', 'Quinolines', 'Succinates']
| 4,164,898
|
[['D02.540.576.500.750'], ['D01.248.497.158.291', 'D01.625.400.100'], ['D08.244', 'D12.776.422.220'], ['D03.383.663.283.446.520.203', 'D03.633.100.150.446.520.203'], ['D02.455.426.559.389.657.566.304', 'D02.640.743.304'], ['G02.111.248', 'G03.295.531.403', 'G03.493.350'], ['D01.268.406', 'D01.362.340'], ['D03.633.100.759.646.138.694', 'D08.211.589', 'D13.695.667.138.694', 'D13.695.827.068.694'], ['G02.111.665.550', 'G03.295.631', 'G03.796.550'], ['B03.440.400.425.625.625', 'B03.660.250.580.590'], ['D03.633.100.810'], ['D02.241.081.337.759']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
The effects of HDV-insulin on carbohydrate metabolism in Type 1 diabetic patients.
|
The aim of this study was to compare the metabolic effects of a single equimolar subcutaneous injection of hepatic directed vesicle-insulin (HDV-insulin) and regular insulin on glucose levels and intermediary metabolism during a 75-g oral glucose tolerance test (OGTT). Nine Type 1 diabetic patients underwent two experiments separated by 4 weeks. Each experimental protocol consisted of an identical evening meal followed by overnight euglycemic control achieved by a continuous low-dose insulin infusion. The next morning a subcutaneous injection (0.1 U/kg) of HDV-insulin or regular insulin was administered 30 min before a 75-g OGTT. The overnight basal insulin infusion was maintained unaltered throughout the 150-min OGTT. Plasma glucose, glucoregulatory hormones (insulin, glucagon, cortisol), and intermediary metabolites (lactate, alanine, glycerol, NEFA, beta-hydroxybutyrate) were measured to assess the metabolic effects of the two insulin preparations. Compared to regular insulin, an equivalent subcutaneous dose of HDV-insulin significantly lowered glucose levels during OGTT (mean reduction 2.2+/-0.4 mmol/l; P<.005). Plasma levels of insulin and glucagon were equivalent during both series of experiments. Blood lactate, glycerol and plasma NEFA levels were not different during OGTT indicating similar peripheral action of the insulins. beta-Hydroxybutyrate levels were significantly reduced (P<.05) following HDV-insulin supporting a preferential hepatic action of the preparation. We conclude that HDV-insulin can significantly lower plasma glucose excursions compared to an equivalent dose of regular insulin during an OGTT in Type 1 diabetic patients. The metabolic profile of equivalent peripheral insulin, glucagon and glycerol levels but reduced beta-hydroxybutyrate values support a hepatospecific effect of HDV-insulin.
|
['Adult', 'Animals', 'Blood Glucose', 'Carbohydrate Metabolism', 'Diabetes Mellitus, Type 1', 'Female', 'Glucose Tolerance Test', 'Humans', 'Hydrocortisone', 'Hypoglycemic Agents', 'Insulin', 'Liposomes', 'Male', 'Mice']
| 11,522,495
|
[['M01.060.116'], ['B01.050'], ['D09.947.875.359.448.500'], ['G02.111.158', 'G03.191'], ['C18.452.394.750.124', 'C19.246.267', 'C20.111.327'], ['E01.370.225.124.100.355', 'E01.370.374.355', 'E05.200.124.100.355'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D04.210.500.745.745.654.600', 'D06.472.040.585.353.476', 'D06.472.040.585.478.392'], ['D27.505.696.422'], ['D06.472.699.587.200.500.625', 'D12.644.548.586.200.500.625'], ['D25.479.517', 'D26.255.260.517', 'J01.637.051.479.517', 'J01.637.087.500.517'], ['B01.050.150.900.649.313.992.635.505.500']]
|
['Named Groups [M]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Technology, Industry, and Agriculture [J]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
|
Cerebrospinal fluid brain injury biomarkers in children: a multicenter study.
|
BACKGROUND: Cerebrospinal fluid (CSF) biomarkers reflecting neuronal and astroglial injury, such as total tau (T-tau), glial fibrillary acidic protein (GFAP), and neurofilament light (NFL), have been extensively investigated in neurologic diseases in adults, but no large study has investigated these biomarkers in children.METHODS: This study presents a detailed evaluation of CFS T-tau, GFAP, NFL, and CSF:albumin ratio in a large cohort of pediatric patients. This is a retrospective multicenter study on pediatric patients aged <16 years (n = 607), where neuronal injury biomarkers T-tau, GFAP, NFL, and CSF albumin ratio were analyzed during 2000-2010 at the Clinical Neurochemistry Laboratory, Sahlgrenska University Hospital, Sweden. The patients were grouped into eight categories: epilepsy, infectious and inflammatory central nervous system disorders, progressive encephalopathy, static encephalopathy, tumors, movement disorders, miscellaneous disorders, and a control group.RESULTS: T-tau, GFAP, and NFL were increased in progressive encephalopathy (P < 0.001), epilepsy (P < 0.001), and infectious and inflammatory central nervous system disorders (P < 0.001) compared with controls. T-tau was the biomarker with the highest diagnostic accuracy with the area under the curve of 0.83 (95% confidence interval (CI), 0.77-0.90; P < 0.0001) for progressive encephalopathy followed by epilepsy 0.80 (95% CI, 0.75-0.87; P < 0.0001). The combination of all four biomarkers further improved the area under the curve for the progressive encephalopathy 0.87 (95% CI, 0.77-0.89; P < 0.0001), followed by epilepsy 0.81 (95% CI, 0.74-0.80; P = 0.030). The combination of the biomarkers also separated progressive from static encephalopathy 0.88 (95% CI, 0.83-0.93; P < 0.0001).CONCLUSIONS: CSF T-tau, GFAP, and NFL are differently altered across different neurologic diseases in children. Importantly, the biomarker pattern distinguishes between progressive and static neurologic disorders.
|
['Adolescent', 'Biomarkers', 'Brain Injuries', 'Child', 'Child, Preschool', 'Cohort Studies', 'Female', 'Glial Fibrillary Acidic Protein', 'Humans', 'Infant', 'Male', 'Neurofilament Proteins', 'Retrospective Studies', 'tau Proteins']
| 23,827,424
|
[['M01.060.057'], ['D23.101'], ['C10.228.140.199', 'C10.900.300.087', 'C26.915.300.200'], ['M01.060.406'], ['M01.060.406.448'], ['E05.318.372.500.750', 'N05.715.360.330.500.750', 'N06.850.520.450.500.750'], ['D05.750.078.593.400', 'D12.776.220.475.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703'], ['D05.750.078.593.630', 'D12.776.220.475.630', 'D12.776.631.630'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['D12.776.220.600.450.510', 'D12.776.631.560.510']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Enhanced protective efficacy of H5 subtype influenza vaccine with modification of the multibasic cleavage site of hemagglutinin in retroviral pseudotypes.
|
Traditionally, the multibasic cleavage site (MBCS) of surface protein H5-hemagglutinin (HA) is converted to a monobasic one so as to weaken the virulence of recombinant H5N1 influenza viruses and to produce inactivated and live attenuated vaccines. Whether such modification benefits new candidate vaccines has not been adequately investigated. We previously used retroviral vectors to generate wtH5N1 pseudotypes containing the wild-type HA (wtH5) from A/swine/Anhui/ca/2004 (H5N1) virus. Here, we generated mtH5N1 pseudotypes, which contained a mutant-type HA (mtH5) with a modified monobasic cleavage site. Groups of mice were subcutaneously injected with the two types of influenza pseudotypes. Compared to the group immunized with wtH5N1 pseudotypes, the inoculation of mtH5N1 pseudotypes induced significantly higher levels of HA specific IgG and IFN-ã in immunized mice, and enhanced protection against the challenge of mouse-adapted avian influenza virus A/Chicken/Henan/12/2004 (H5N1). This study suggests modification of the H5-hemagglutinin MBCS in retroviral pseudotypes enhances protection efficacy in mice and this information may be helpful for development of vaccines from mammalian cells to fight against H5N1 influenza viruses.
|
['Animals', 'Antibodies, Viral', 'Genetic Vectors', 'Hemagglutinin Glycoproteins, Influenza Virus', 'Influenza Vaccines', 'Interferon-gamma', 'Leukocytes, Mononuclear', 'Mice', 'Mutant Proteins', 'Recombinant Proteins', 'Retroviridae', 'Vaccines, Synthetic']
| 23,728,771
|
[['B01.050'], ['D12.776.124.486.485.114.254', 'D12.776.124.790.651.114.254', 'D12.776.377.715.548.114.254'], ['G05.360.337'], ['D12.776.964.970.880.345.500'], ['D20.215.894.899.302'], ['D12.644.276.374.440.893', 'D12.644.276.374.480.615.350', 'D12.776.467.374.440.893', 'D12.776.467.374.480.615.350', 'D23.529.374.440.893', 'D23.529.374.480.615.350'], ['A11.118.637.555', 'A15.145.229.637.555', 'A15.382.490.555'], ['B01.050.150.900.649.313.992.635.505.500'], ['D12.776.602'], ['D12.776.828'], ['B04.613.807', 'B04.820.650'], ['D12.776.828.868', 'D20.215.894.865', 'D23.050.865']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Oxygen inhibits neuronal activation in the trigeminocervical complex after stimulation of trigeminal autonomic reflex, but not during direct dural activation of trigeminal afferents.
|
OBJECTIVE: To understand the mechanism of action of oxygen treatment in cluster headache.BACKGROUND: Trigeminal autonomic cephalalgias, including cluster headache, are characterized by unilateral head pain in association with ipsilateral cranial autonomic features. They are believed to involve activation of the trigeminovascular system and the parasympathetic outflow to the cranial vasculature from the superior salivatory nucleus (SuS) projections through the sphenopalatine ganglion, via the greater petrosal nerve of the VIIth (facial) cranial nerve. Cluster headache is remarkably responsive to treatment with oxygen, and yet our understanding of its mode of action is unknown.METHODS: Combining models of trigeminovascular nociception and a novel approach that activates the trigeminal-autonomic reflex, using SuS/facial nerve stimulation, we explored the effect of oxygen on trigeminal nerve activation as well as on autonomic responses through blood flow observations of the lacrimal duct/sac.RESULTS: Meningeal vasodilation and neuronal firing in the trigeminocervical complex (TCC), in response to dural electrical stimulation, was unaffected by treatment with 100% oxygen. Stimulation of the SuS via the facial nerve caused only marginal changes in dural blood vessel diameter, but did result in evoked firing in the TCC. Two populations of neurons were characterized, those responsive to 100% oxygen treatment, with a maximal inhibition of 33%, 20 minutes after the start of oxygen treatment (t(15) = 4.4, P < .0001). A second population of neurons were not inhibited by oxygen and tended to have shorter latency. Oxygen also inhibited evoked blood flow changes in the lacrimal sac/duct caused by SuS stimulation.CONCLUSIONS: The data provide the first systematic, experimental evidence for a mechanism of action of oxygen in cluster headache. The data show oxygen has no direct effect on trigeminal afferents, acting specifically on the parasympathetic/facial nerve projections to the cranial vasculature to inhibit both evoked trigeminovascular activation and activation of the autonomic pathway during cluster headache attacks. Moreover, the studies begin to characterize a novel laboratory model for the most painful primary headache syndrome known--cluster headache.
|
['Action Potentials', 'Animals', 'Cerebral Arteries', 'Cerebrovascular Circulation', 'Cluster Headache', 'Disease Models, Animal', 'Dura Mater', 'Electric Stimulation', 'Facial Nerve', 'Male', 'Oxygen', 'Parasympathetic Nervous System', 'Rats', 'Rats, Sprague-Dawley', 'Reflex, Abnormal', 'Sensory Receptor Cells', 'Trigeminal Caudal Nucleus', 'Trigeminal Nerve', 'Vasodilation']
| 19,719,541
|
[['G04.580.100', 'G07.265.675.100', 'G11.561.570.100'], ['B01.050'], ['A07.015.114.228'], ['G09.330.100.159'], ['C10.228.140.546.399.937.500'], ['C22.232', 'E05.598.500', 'E05.599.395.080'], ['A08.186.566.395'], ['E05.723.402'], ['A08.800.050.050.275', 'A08.800.050.600.149', 'A08.800.800.060.275', 'A08.800.800.120.250'], ['D01.268.185.550', 'D01.362.670'], ['A08.800.050.600'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.750'], ['C10.597.704', 'C23.888.592.717', 'E01.370.376.550.650.655', 'E01.370.600.550.650.655', 'G11.561.731.587'], ['A08.675.650.915', 'A08.800.950', 'A11.671.650.915'], ['A08.186.211.132.810.591.500.875.500', 'A08.186.211.132.931.920.927'], ['A08.800.800.120.760'], ['G09.330.380.928']]
|
['Phenomena and Processes [G]', 'Organisms [B]', 'Anatomy [A]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Propositions for refinement of the hybrid surgical technique for treatment of thoraco-abdominal aortic aneurysm.
|
Traditional open repair of thoraco-abdominal aortic aneurysms Crawford type II-IV carries a high perioperative risk and mortality. The hybrid technique for combined surgical and endovascular treatment offers an interesting alternative with reduced risk of paraparesis and possibly a reduced mortality rate. Propositions for refinement of this approach are outlined based on a single centre experience.
|
['Aortic Aneurysm, Abdominal', 'Aortic Aneurysm, Thoracic', 'Blood Vessel Prosthesis', 'Humans', 'Vascular Surgical Procedures']
| 18,575,038
|
[['C14.907.055.239.075', 'C14.907.109.139.075'], ['C14.907.055.239.125', 'C14.907.109.139.125'], ['E07.695.110'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E04.100.814']]
|
['Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
An autoradiographic study of the implantation of transferred mouse blastocysts.
|
Mouse blastocysts collected on day 4 were cultured in [3H]thymidine (0.01 muCi/ml) for 24 h and transferred to the uteri of pseudopregnant recipients. Autoradiography revealed that when such blastocysts were allowed to develop for 48 h in utero, label was apparent in the nuclei of decidual cells. The experimental conditions were physiological since blastocysts developed into normal offspring when gestation was allowed to proceed in pseudopregnant recipient animals. The transfer of foetal DNA into maternal decidual cells may be of important immunological significance.
|
['Animals', 'Autoradiography', 'Blastocyst', 'Embryo Implantation', 'Embryo Transfer', 'Female', 'Mice', 'Pseudopregnancy', 'Thymidine', 'Transplantation, Homologous', 'Uterus']
| 614,008
|
[['B01.050'], ['E01.370.225.750.132', 'E05.200.750.132', 'E05.799.256'], ['A16.254.500'], ['G08.686.784.170.104.500'], ['E02.875.800.500', 'E05.820.800.500'], ['B01.050.150.900.649.313.992.635.505.500'], ['G08.686.784.769.887'], ['D03.383.742.680.705', 'D13.570.230.855', 'D13.570.685.705'], ['E04.936.864'], ['A05.360.319.679']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
A new bluetongue virus serotype isolated in Kenya.
|
An apparently new strain of bluetongue virus was first isolated in Kenya in 1965 and since, has been obtained on 7 further occasions from diseased sheep during clinical outbreaks of disease. It proved to be serologically different from the 16 bluetongue virus strains then held at this laboratory. The virus was modified by passage in embryonated hens eggs to produce a live virus strain suitable for inclusion in a polyvalent vaccine. Recent neutralisation tests, carried out with 24 guinea pig immune sera prepared at Pirbright against the currently known World serotypes, have confirmed the earlier results and show that it is different from any of the existing serotypes.
|
['Animals', 'Antibodies, Viral', 'Bluetongue', 'Bluetongue virus', 'Disease Outbreaks', 'Fluorescent Antibody Technique', 'Immune Sera', 'Kenya', 'Neutralization Tests', 'Serotyping', 'Sheep', 'Vaccines, Attenuated', 'Viral Vaccines']
| 1,319,624
|
[['B01.050'], ['D12.776.124.486.485.114.254', 'D12.776.124.790.651.114.254', 'D12.776.377.715.548.114.254'], ['C01.920.500.125', 'C01.925.081.125', 'C01.925.782.791.315', 'C22.836.120'], ['B04.820.223.719.550.100'], ['N06.850.290'], ['E01.370.225.500.607.512.240', 'E01.370.225.750.551.512.240', 'E05.200.500.607.512.240', 'E05.200.750.551.512.240', 'E05.478.583.375'], ['A12.207.152.846.500', 'D12.776.124.486.485.114.573', 'D12.776.124.790.651.114.573', 'D12.776.377.715.548.114.573', 'D20.215.401'], ['Z01.058.290.120.400'], ['E01.370.225.812.735.550', 'E05.200.812.735.550', 'E05.478.594.760.550'], ['E01.370.225.812.742', 'E01.370.225.875.150.125.890', 'E05.200.812.742', 'E05.200.875.150.125.890', 'E05.478.594.780'], ['B01.050.150.900.649.313.500.380.791'], ['D20.215.894.811'], ['D20.215.894.899']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Health Care [N]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Geographicals [Z]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
|
Replantation of a transmetacarpal amputation. A case report.
|
A case is presented of a 23-year-old male who sustained a traumatic transmetacarpal amputation of his nondominant hand. The injury consisted of complete severance of structures distal to the midpalm. Microsurgical reconstruction involved the primary repair of arteries, veins, nerves, extensor and flexor tendons, and metacarpal fractures. Skeletal reconstruction also employed a primary Swanson prosthesis for the fifth metacarpophalangeal (MP) joint. Early postoperative range-of-motion exercises were encouraged, with the achievement of a functionally capable replanted extremity. The general management of an amputation injury is also discussed, as it applies to a community hospital environment.
|
['Adult', 'Amputation, Traumatic', 'Fracture Fixation, Internal', 'Hand Injuries', 'Humans', 'Joint Prosthesis', 'Male', 'Metacarpus', 'Microsurgery', 'Motor Skills', 'Replantation', 'Wound Healing']
| 4,057,161
|
[['M01.060.116'], ['C26.062'], ['E04.555.300.300'], ['C26.448'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E07.695.400'], ['A01.378.800.667.572'], ['E04.494', 'E05.591.580'], ['F02.808.260'], ['E04.936.494'], ['G16.762.891']]
|
['Named Groups [M]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Anatomy [A]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Health-related quality of life and determinant factors in celiac disease. A population-based analysis of adult patients in Spain.
|
BACKGROUND: Celiac disease (CD) has a negative impact on the health-related quality of life (HRQL) of affected patients. Although HRQL and its determinants have been examined in Spanish CD patients specifically recruited in hospital settings, these aspects of CD have not been assessed among the general Spanish population.METHODS: An observational, transversal study of a non-randomized, representative sample of adult celiac patients throughout all of Spain's Autonomous Regions. Subjects were recruited through celiac patient associations. A Spanish version of the self-administered Celiac Disease-Quality of Life (CD-QOL) questionnaire was used. Determinant factors of HRQL were assessed with the aid of multivariate analysis to control for confounding factors.RESULTS: We analyzed the responses provided by 1,230 patients, 1,092 (89.2%) of whom were women. The overall mean value for the CD-QOL index was 56.3 ± 18.27 points. The dimension that obtained the most points was dysphoria, with 81.3 ± 19.56 points, followed by limitations with 52.3 ± 23.43 points; health problems, with 51.6 ± 26.08 points, and inadequate treatment, with 36.1 ± 21.18 points. Patient age and sex, along with time to diagnosis, and length of time on a gluten-free diet were all independent determinant factors of certain dimensions of HRQL: women aged 31 to 40 expressed poorer HRQL while time to diagnosis and length of time on a gluten-free diet were determinant factors for better HRQL scores.CONCLUSIONS: The HRQL of adult Spanish celiac subjects is moderate, improving with the length of time patients remain on a gluten-free diet.
|
['Adolescent', 'Adult', 'Age Factors', 'Aged', 'Celiac Disease', 'Cross-Sectional Studies', 'Diet, Gluten-Free', 'Female', 'Health Status', 'Humans', 'Male', 'Middle Aged', 'Population', 'Quality of Life', 'Sex Factors', 'Spain', 'Surveys and Questionnaires', 'Young Adult']
| 26,901,502
|
[['M01.060.057'], ['M01.060.116'], ['N05.715.350.075', 'N06.850.490.250'], ['M01.060.116.100'], ['C06.405.469.637.250', 'C18.452.603.250'], ['E05.318.372.500.875', 'N05.715.360.330.500.875', 'N06.850.520.450.500.875'], ['E02.642.249.265', 'G07.203.650.240.265'], ['I01.240.425', 'N01.224.425', 'N06.850.505.400.425'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['N01.600'], ['I01.800', 'K01.752.400.750', 'N06.850.505.400.425.837'], ['N05.715.350.675', 'N06.850.490.875'], ['Z01.542.846'], ['E05.318.308.980', 'N05.715.360.300.800', 'N06.850.520.308.980'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Health Care [N]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Organisms [B]', 'Humanities [K]', 'Geographicals [Z]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 1
|
Indicators for healthy ageing--a debate.
|
Definitions of healthy ageing include survival to a specific age, being free of chronic diseases, autonomy in activities of daily living, wellbeing, good quality of life, high social participation, only mild cognitive or functional impairment, and little or no disability. The working group Epidemiology of Ageing of the German Association of Epidemiology organized a workshop in 2012 with the aim to present different indicators used in German studies and to discuss their impact on health for an ageing middle-European population. Workshop presentations focused on prevalence of chronic diseases and multimorbidity, development of healthy life expectancy at the transition to oldest-age, physical activity, assessment of cognitive capability, and functioning and disability in old age. The communication describes the results regarding specific indicators for Germany, and hereby contributes to the further development of a set of indicators for the assessment of healthy ageing.
|
['Aged', 'Aged, 80 and over', 'Aging', 'Chronic Disease', 'Cognition', 'Disabled Persons', 'Germany', 'Humans', 'Life Expectancy', 'Motor Activity', 'Quality of Life']
| 24,317,381
|
[['M01.060.116.100'], ['M01.060.116.100.080'], ['G07.345.124'], ['C23.550.291.500'], ['F02.463.188'], ['M01.150'], ['Z01.542.315'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.308.985.450', 'N01.224.935.464', 'N06.850.505.400.975.450', 'N06.850.520.308.985.450'], ['F01.145.632', 'G11.427.410.698'], ['I01.800', 'K01.752.400.750', 'N06.850.505.400.425.837']]
|
['Named Groups [M]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Geographicals [Z]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Humanities [K]']
| 0
| 1
| 1
| 0
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 1
|
Contribution of virtual endoscopy to the investigation of laryngotracheal pathological conditions.
|
Three-dimensional CT scan imaging obtained by using helicoidal CT scan provides the basis for an endoscopic exam said to be virtual since no invasive procedure is actually performed. Compared to optical endoscopy this easily accessible exam offers additional information especially for the analysis of the infraglottic and tracheal areas, which are two anatomically rigid segments. This property facilitates their three-dimensional reconstruction. Our study encompassed 6 patients presenting with a stenosis of the laryngotracheal tract. In 5 of them it was possible to correlate optical and virtual endoscopic imaging. Coupling both exams significantly improved the diagnostic investigation and facilitated the management of the disease. However, the real contribution of virtual endoscopy to the exploration of tumoral conditions still remains to be determined given the low degree of tissue resolution. As a consequence parietal and extraparietal lesional spreading is more accurately assessed by axial scan imaging.
|
['Adult', 'Anastomosis, Surgical', 'Endoscopy', 'Female', 'Humans', 'Laryngostenosis', 'Male', 'Middle Aged', 'Periosteum', 'Tomography, X-Ray Computed', 'Tracheal Stenosis', 'User-Computer Interface']
| 12,624,504
|
[['M01.060.116'], ['E04.035'], ['E01.370.388.250', 'E04.502.250'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C08.360.591', 'C09.400.591', 'C16.131.740.658'], ['M01.060.116.630'], ['A10.165.265.746'], ['E01.370.350.350.810', 'E01.370.350.600.350.700.810', 'E01.370.350.700.700.810', 'E01.370.350.700.810.810', 'E01.370.350.825.810.810'], ['C08.907.663'], ['L01.224.900.910']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Diseases [C]', 'Anatomy [A]', 'Information Science [L]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
| 0
|
Dynamic Load Balancing Strategy for Parallel Tumor Growth Simulations.
|
In this paper, we propose a parallel cellular automaton tumor growth model that includes load balancing of cells distribution among computational threads with the introduction of adjusting parameters. The obtained results show a fair reduction in execution time and improved speedup compared with the sequential tumor growth simulation program currently referenced in tumoral biology. The dynamic data structures of the model can be extended to address additional tumor growth characteristics such as angiogenesis and nutrient intake dependencies.
|
['Algorithms', 'Cell Proliferation', 'Computational Biology', 'Computer Simulation', 'Humans', 'Models, Biological', 'Neoplasms', 'Tumor Cells, Cultured']
| 30,763,265
|
[['G17.035', 'L01.224.050'], ['G04.161.750', 'G07.345.249.410.750'], ['H01.158.273.180', 'L01.313.124'], ['L01.224.160'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.599.395'], ['C04'], ['A11.251.860']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Disciplines and Occupations [H]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Anatomy [A]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
|
[Possibilities of health status examination in young men in the Czech Republic].
|
BACKGROUND: The goal of this work was to point to the possibilities of health status examination in young men in the Czech Republic. A sample of young men, volunteers, conscripts (servicemen) at the time, was studied within the presented work.METHODS: A questionnaire--screening protocol--was filled in by 104 young men, 83 of them subsequently underwent a clinical examination. The participants expressed their interest in the research and the chosen form of primary prevention during directed talks; 64 participants took part in these talks.RESULTS: Analyzing the results we found out the following: the values of pulmonary function testing prove that despite their relatively low age, young men have unsatisfactory pulmonary function; the physical condition of the generation of young men, however, is satisfactory. Although young men do consume fruits and vegetables, they nevertheless have serious shortcomings in their dietary habits. Our clinical examination points to the symptoms of vitamin deficiency.CONCLUSIONS: The chosen method of work including the assessment and the possibility of making use of primary prevention should be reproducible and usable for a wide range of our young population.
|
['Adult', 'Czech Republic', 'Humans', 'Male', 'Military Personnel', 'Physical Examination', 'Physical Fitness', 'Young Adult']
| 21,560,449
|
[['M01.060.116'], ['Z01.542.248.395'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.526.625'], ['E01.370.600'], ['G11.427.685', 'I03.450.642.845.054.800', 'N01.400.545'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Geographicals [Z]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]']
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 1
|
Folate content in commercial white and whole wheat sandwich breads.
|
After the U.S. mandate of folic acid fortification of enriched grain products, a report indicated higher than expected fortification. Limited information is available on folic acid in enriched products. We measured the folate content in 92 sandwich breads (46 white breads and 46 whole wheat breads) in Birmingham, Alabama, during 2001-2003. The mean folate content in white bread declined significantly from 2001 to 2002 or 2003, whereas the decline in folate content in whole wheat bread containing enriched flour was not significant. White bread contained significantly more folate than whole wheat bread containing enriched flour in 2001 and 2003. In 2002 and 2003, >40% of breads made of enriched flour contained <115 microg of folate/100 g and >70% contained <160 microg/100 g. These percentages were markedly higher than those in 2001. Our data suggest that folic acid in breads containing enriched flour declined after 2001 and monitoring of fortification may be necessary.
|
['Bread', 'Folic Acid', 'Food, Fortified', 'Triticum']
| 15,453,710
|
[['G07.203.300.100', 'J02.500.100'], ['D03.633.100.733.631.400'], ['G07.203.300.515', 'J02.500.515'], ['B01.650.940.800.575.912.250.822.918']]
|
['Phenomena and Processes [G]', 'Technology, Industry, and Agriculture [J]', 'Chemicals and Drugs [D]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Four-Meter Gait Speed: Normative Values and Reliability Determined for Adults Participating in the NIH Toolbox Study.
|
OBJECTIVES: To establish reference values and determine test-retest reliability for usual and maximal 4-meter gait speed.DESIGN: Cross-sectional observational study.SETTING: Offices in 10 geographically dispersed cities in the United States.PARTICIPANTS: Men and women (N=1320), aged 18 to 85 years, enrolled in the National Institutes of Health Toolbox norming study.INTERVENTION: Not applicable.MAIN OUTCOME MEASURES: Specifically used were data from men and women who were timed over 4 meters (after a static start) while walking at their usual and maximum speeds. Norms for usual and maximum gait speed were derived using data from 1320 participants. Test-retest reliability for 164 participants was described using paired t tests, intraclass correlation coefficients (ICCs), and minimal detectable changes (MDCs).RESULTS: Mean usual speed was 1.12 meters per second, whereas mean maximum speed was 1.61 meters per second. As a general linear model showed 4-meter gait speed to differ significantly according to gait condition (speed), sex, and age group; estimates of normal were calculated accordingly. The usual speed of 80- to 85-year-old women was lowest at 0.95 meters per second; the maximum speed of 18- to 29-year-old men was highest at 1.85 meters per second. Test-retest measures did not differ significantly, but the ICCs were only fair and the MDCs were high.CONCLUSIONS: Normative reference values provided herein may be helpful in interpreting measurements of 4-meter gait speed obtained from adult men and women. The limited reliability of the gait speed measurements, however, limits their usefulness in making judgments regarding change.
|
['Adolescent', 'Adult', 'Aged', 'Aged, 80 and over', 'Cross-Sectional Studies', 'Female', 'Health', 'Healthy Volunteers', 'Humans', 'Limit of Detection', 'Male', 'Middle Aged', 'Reference Values', 'Reproducibility of Results', 'Statistics, Nonparametric', 'United States', 'Walk Test', 'Walking Speed', 'Young Adult']
| 30,092,204
|
[['M01.060.057'], ['M01.060.116'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['E05.318.372.500.875', 'N05.715.360.330.500.875', 'N06.850.520.450.500.875'], ['N01.400'], ['M01.774.500', 'M01.955.236'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.740.872.374', 'N05.715.360.750.725.500', 'N06.850.520.830.872.500'], ['M01.060.116.630'], ['E05.978.810'], ['E05.318.370.725', 'E05.337.851', 'N05.715.360.325.685', 'N06.850.520.445.725'], ['E05.318.740.995', 'N05.715.360.750.760', 'N06.850.520.830.995'], ['Z01.107.567.875'], ['E01.370.370.380.250.500'], ['E01.370.600.250.500', 'G11.427.410.568.900.750.500'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Geographicals [Z]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Rejection sensitivity and suicide ideation among psychiatric inpatients: An integration of two theoretical models.
|
Rejection is a direct threat to an individual's need to belong that has serious consequences for mental health. Rejection sensitivity may explain why some individuals are more likely to perceive rejection in social situations and experience subsequent psychological distress. The current study examined suicide ideation among psychiatric inpatients (N = 103) through the lenses of the rejection sensitivity model and the interpersonal theory of suicide. We hypothesized that rejection sensitivity would be indirectly associated with suicide ideation (i.e., a cognitive-affective reaction to social rejection) through greater perceptions of rejection (i.e., thwarted belongingness and perceived burdensomeness, constructs from the interpersonal theory of suicide), in parallel. Results from bootstrapped parallel mediation regression procedures indicated that the relation between rejection sensitivity and suicide ideation was significantly indirectly associated through the additive effect of thwarted belongingness and perceived burdensomeness, such that greater rejection sensitivity was associated with greater thwarted belongingness and perceived burdensomeness and subsequently greater suicide ideation. Further, rejection sensitivity was significantly indirectly associated with suicide ideation independently through thwarted belongingness, but not perceived burdensomeness. These findings provide support for the rejection sensitivity model and the interpersonal theory of suicide in an effort to advance our conceptualization of suicide risk among psychiatric inpatients.
|
['Adult', 'Aged', 'Female', 'Humans', 'Interpersonal Relations', 'Male', 'Mental Disorders', 'Middle Aged', 'Models, Theoretical', 'Psychological Distance', 'Psychological Theory', 'Risk Factors', 'Suicidal Ideation', 'Young Adult']
| 30,579,182
|
[['M01.060.116'], ['M01.060.116.100'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F01.829.401'], ['F03'], ['M01.060.116.630'], ['E05.599'], ['F01.145.813.630', 'F01.829.316.777'], ['F02.739'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['F01.145.126.980.875.149', 'I01.880.735.856.149'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Organisms [B]', 'Psychiatry and Psychology [F]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Anthropology, Education, Sociology, and Social Phenomena [I]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 0
|
Effects of ethyl apovincaminate on the central nervous system.
|
Side effects of ethyl apovincaminate (RGH-4405, Cavinton), a cerebral vasodilatory agent, on the central nervous system were studied on mice and rats. Anticonvulsive effect in electroshock was observed as major CNS effect, locomotor activity was reduced due to muscle relaxant effect. The compound had no sedative effect on rodents. Its analgesic effect was negligible. Cavinton had neither antidepressive, nor anticholinergic activity.
|
['Animals', 'Brain', 'Female', 'Hexobarbital', 'Lethal Dose 50', 'Male', 'Mice', 'Motor Activity', 'Rats', 'Seizures', 'Sleep', 'Vasodilator Agents', 'Vinca Alkaloids']
| 1,037,217
|
[['B01.050'], ['A08.186.211'], ['D03.383.742.698.253.317'], ['E05.940.402', 'G07.225.500', 'G07.690.773.875.750', 'G07.690.936.500.750'], ['B01.050.150.900.649.313.992.635.505.500'], ['F01.145.632', 'G11.427.410.698'], ['B01.050.150.900.649.313.992.635.505.700'], ['C10.597.742', 'C23.888.592.742'], ['F02.830.855', 'G11.561.803'], ['D27.505.954.411.918'], ['D03.132.436.681.827', 'D03.633.100.473.402.681.827', 'D03.633.100.496.500.500.681.827']]
|
['Organisms [B]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Psychiatry and Psychology [F]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Application of an optimized total N-nitrosamine (TONO) assay to pools: placing N-nitrosodimethylamine (NDMA) determinations into perspective.
|
Although N-nitrosodimethylamine (NDMA) has been the most prevalent N-nitrosamine detected in disinfected waters, it remains unclear whether NDMA is indeed the most significant N-nitrosamine or just one representative of a larger pool of N-nitrosamines. A widely used assay applied to quantify nitrite, S-nitrosothiols, and N-nitrosamines in biological samples involves their reduction to nitric oxide by acidic tri-iodide, followed by chemiluminescent detection of the evolved nitric oxide in the gas phase. We here describe an adaptation of this method for analyzing total N-nitrosamine (TONO) concentrations in disinfected pools. Optimal sensitivity for N-nitrosamines was obtained using a reduction solution containing 13.5 mL glacial acetic acid and 1 mL of an aqueous 540 g/L iodide and 114 g/L iodine solution held at 80 degrees C. The method detection limit for N-nitrosamines was 110 nM using 100 microL sample injections and NDMA as a standard. N-nitrosamines featuring a range of polarities were converted to nitric oxide with 75-103% efficiency compared to NDMA. Evaluation of potential interfering species indicated that only nitrite and S-nitrosothiols were a concern, but both interferences were effectively eliminated using group-specific sample pretreatments previously employed for biological samples. To evaluate the low TONO concentrations anticipated for pools, 1 L samples were extracted by continuous liquid-liquid extraction with ethyl acetate for 24 h, and concentrated to 1 mL. N-nitrosamine recovery during extraction ranged from 37-75%, and there was a potential for artifactual nitrosation of amines during solvent reflux in the presence of significant nitrite concentrations, but not at the low nitrite concentrations prevalent in most pools. Using the 1000-fold concentration factor and 56% average extraction efficiency, the method detection limit would be 62 pM (5 ng/L as NDMA). The TONO assay was applied to six pools and their common tap water source in conjunction with analysis for specific nitrosamines. Even accounting for the range of N-nitrosamine extraction recoveries, NDMA accounted for an average of only 13% (range 3-46%) of the total nitrosamine pool.
|
['Acetates', 'Acetic Acid', 'Dimethylnitrosamine', 'Disinfection', 'Humans', 'Hydrogen-Ion Concentration', 'Models, Chemical', 'Neoplasms', 'Nitrosamines', 'Risk', 'Swimming Pools', 'Temperature', 'Time Factors', 'Water Pollutants, Chemical', 'Water Purification']
| 20,355,696
|
[['D02.241.081.018', 'D10.251.400.045'], ['D02.241.081.018.165', 'D10.251.400.045.500'], ['D02.654.442.225'], ['N06.850.780.200.450.850.375'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G02.300'], ['E05.599.495'], ['C04'], ['D02.654.442'], ['E05.318.740.600.800', 'G17.680.750', 'N05.715.360.750.625.700', 'N06.850.520.830.600.800'], ['J03.925.830'], ['G01.906.595', 'G16.500.275.063.725.710', 'G16.500.750.775.710', 'N06.230.150.450', 'N06.230.300.100.725.710'], ['G01.910.857'], ['D27.888.284.903.655'], ['N06.850.780.200.800.800.900.900', 'N06.850.860.510.900.900']]
|
['Chemicals and Drugs [D]', 'Health Care [N]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Technology, Industry, and Agriculture [J]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
|
[Determination of diphenyl and o-phenylphenol in agricultural products by GC/MS].
|
A simple determination method of diphenyl (DP) and o-phenylphenol (OPP) in agricultural products by GC/MS was examined. DP and OPP were extracted with ethyl acetate in the presence of anh. sodium sulfate. After addition of n-butanol, the extract solution was concentrated. Clean-up was achieved by shaking with graphitized bulk carbon (Supelclean ENVI-Carb). Addition of polyethylene glycol sharpened the OPP peak on GC/MS analysis. The recoveries from 9 kinds of agricultural products spiked at 0.01 and 0.5 microg/g each were mostly in the range of 70 to 120%, except for 50% recovery of OPP from barley spiked at 0.01 microg/g. The quantification limits (S/N > or =10) of DP and OPP were 0.0013 and 0.005 microg/g (0.0025 and 0.01 microg/g in barley and soybean), respectively.
|
['Biphenyl Compounds', 'Chromatography, Gas', 'Edible Grain', 'Fruit', 'Mass Spectrometry', 'Vegetables']
| 19,155,596
|
[['D02.455.426.559.389.185'], ['E05.196.181.349'], ['A18.024.500.750.500', 'B01.650.160.250', 'B01.650.510.250', 'G07.203.300.300.550', 'G07.203.300.775.500', 'J02.500.300.550', 'J02.500.775.500'], ['A18.024.500', 'G07.203.300.562', 'J02.500.562'], ['E05.196.566'], ['B01.650.160.956', 'B01.650.510.956', 'G07.203.300.850', 'J02.500.850']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Technology, Industry, and Agriculture [J]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Differential inhibition of receptor activation by two mouse monoclonal antibodies specific for the human leukotriene B4 receptor, BLT1.
|
The inflammatory mediator leukotriene B(4) (LTB(4)) binds to and activates a G-protein-coupled receptor named BLT(1). We have previously produced two monoclonal antibodies, named 7B1 and 14F11, that bind specifically to this receptor. Using a HeLa cell line expressing human BLT(1), we find that both antibodies inhibit LTB(4)-induced calcium release, and activation of a MAP-kinase-sensitive luciferase reporter system. The normal chemotactic movement of polymorphonuclear cells towards higher LTB(4) concentrations was also strongly inhibited by both antibodies. Neither antibody was found to activate BLT(1), and experiments using cyclic peptide fragments of the BLT(1) n-terminal and extracellular loops showed that these antibodies bind only to complex epitopes in the tertiary, membrane bound, conformation of the receptor protein. In ligand binding experiments, 7B1 was found to be a competitive antagonist, while 14F11 was a noncompetitive antagonist that inhibited receptor activation, but not agonist (LTB(4)) binding. 14F11 will be a useful tool for studying the mechanisms of receptor activation.
|
['Animals', 'Antibodies, Monoclonal', 'Antibody Specificity', 'Antigen-Antibody Reactions', 'Binding, Competitive', 'Calcium', 'Enzyme-Linked Immunosorbent Assay', 'Flow Cytometry', 'Fluorescence Polarization', 'Gene Expression', 'HeLa Cells', 'Humans', 'Leukotriene Antagonists', 'Leukotriene B4', 'Luciferases', 'Mice', 'Receptors, G-Protein-Coupled', 'Receptors, Leukotriene B4']
| 14,636,832
|
[['B01.050'], ['D12.776.124.486.485.114.224', 'D12.776.124.790.651.114.224', 'D12.776.377.715.548.114.224'], ['G12.100'], ['G12.122'], ['E05.196.080', 'G02.111.084', 'G02.111.570.120.309'], ['D01.268.552.100', 'D01.552.539.288', 'D23.119.100'], ['E05.478.566.350.170', 'E05.478.566.380.360', 'E05.478.583.400.170', 'E05.601.470.350.170', 'E05.601.470.380.360'], ['E01.370.225.500.363.342', 'E01.370.225.500.386.350', 'E05.196.712.516.600.240.350', 'E05.200.500.363.342', 'E05.200.500.386.350', 'E05.242.363.342', 'E05.242.386.350'], ['E05.196.712.516.600.390'], ['G05.297'], ['A11.251.210.190.400', 'A11.251.860.180.400', 'A11.436.340'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D06.347.565', 'D27.505.696.399.450.565'], ['D10.251.355.255.100.450.411', 'D10.251.355.310.166.887.411', 'D23.469.050.175.450.415'], ['D08.811.682.517', 'D12.776.532.510'], ['B01.050.150.900.649.313.992.635.505.500'], ['D12.776.543.750.695'], ['D12.776.543.750.695.200.450.300']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
A pilot six-week randomized controlled trial of oxytocin on social cognition and social skills in schizophrenia.
|
The current study explored whether oxytocin can improve social cognition and social skills in individuals with schizophrenia using a six-week, double-blind design. Fourteen participants with schizophrenia were randomized to receive either intranasal oxytocin or a placebo solution and completed a battery of social cognitive, social skills and clinical psychiatric symptom measures. Results showed within group improvements in fear recognition, perspective taking, and a reduction in negative symptoms in the oxytocin group. These preliminary findings indicate oxytocin treatment may help improve certain components of functioning in schizophrenia. Implications for the treatment of social functioning in schizophrenia are discussed.
|
['Administration, Intranasal', 'Adult', 'Antipsychotic Agents', 'Cognition', 'Double-Blind Method', 'Female', 'Humans', 'Male', 'Oxytocin', 'Pilot Projects', 'Psychiatric Status Rating Scales', 'Schizophrenia', 'Schizophrenic Psychology', 'Social Behavior', 'Social Skills', 'Treatment Outcome']
| 24,799,299
|
[['E02.319.267.120.655.500'], ['M01.060.116'], ['D27.505.696.277.950.040', 'D27.505.954.427.210.950.040', 'D27.505.954.427.700.872.331'], ['F02.463.188'], ['E05.318.370.300', 'E05.581.500.300', 'N05.715.360.325.320', 'N06.850.520.445.300'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D06.472.699.631.692.433', 'D12.644.548.691.692.433'], ['E05.318.372.750', 'E05.337.737', 'N05.715.360.330.720', 'N06.850.520.450.720'], ['F04.711.513.653'], ['F03.700.750'], ['F04.824'], ['F01.145.813'], ['F01.145.813.828', 'F01.829.401.737'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Named Groups [M]', 'Chemicals and Drugs [D]', 'Psychiatry and Psychology [F]', 'Health Care [N]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Increased MPV is not a significant predictor for preeclampsia during pregnancy.
|
BACKGROUND: Preeclampsia, defined as the presence of hypertension and proteinuria, is usually related with maternal and neonatal adverse effects. However, the exact predictor of preeclampsia is still lacking. Even though there are some conflicting data, mean platelet value or MPV, that is, platelet ratio with or without Doppler velocimetry was determined as highly sensitive markers for preeclampsia. We aimed to investigate the utility of MPV in prediction of preeclampsia.METHODS: Seventy-four preeclamptic pregnant women (21 in mild, 53 in severe preeclampsia groups) were included in the study. To assess the difference of MPV between preeclamptic, normal pregnant, and healthy control rather than mild and severe preeclamptic pregnant women, we included in the analysis 31 healthy pregnant women and 35 healthy nonpregnant women.RESULTS: Mean age of the preeclamptic patients was 25.3 (17-38) years. Platelet levels were higher in mild preeclampsia (group 1) than severe preeclampsia (group 2), whereas alanine aminotransferase (AST), hemoglobin, and hematocrit level was higher in group 2. MPV levels were found to be similar in groups 1 and 2, MPV level increased from healthy control to preeclamptic women (P = 0.003). MPV:platelet ratio was similar according to the severity of preeclampsia (P = 0.123). Doppler velocimetry did not add an additional benefit to predict preeclampsia or its severity.CONCLUSION: Our results showed that MPV level was higher in the pregnant than the control group. However, MPV did not differ both between mild and severe preeclampsia, and preeclampsia and non-preeclamptic pregnant women.
|
['Adolescent', 'Adult', 'Blood Platelets', 'Female', 'Humans', 'Laser-Doppler Flowmetry', 'Pre-Eclampsia', 'Predictive Value of Tests', 'Pregnancy', 'Statistics, Nonparametric']
| 23,001,987
|
[['M01.060.057'], ['M01.060.116'], ['A11.118.188', 'A15.145.229.188'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.370.475', 'E05.830.500'], ['C13.703.395.249'], ['E05.318.370.800.650', 'N05.715.360.325.700.640', 'N06.850.520.445.800.650'], ['G08.686.784.769'], ['E05.318.740.995', 'N05.715.360.750.760', 'N06.850.520.830.995']]
|
['Named Groups [M]', 'Anatomy [A]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Health Care [N]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Characterization and Inductive Expression Analysis of Interferon Gamma-Related Gene in the Indian Major Carp, Rohu (Labeo rohita).
|
Interferon gamma (IFN-ã) is one of the key cytokines that plays a major role against viral and intracellular bacterial infection. In addition to the IFN-ã gene, teleost fish possess a second copy known as IFN-ã-related (IFN-ãrel) gene. This report describes structural and functional properties of IFN-ãrel gene in the Indian major carp, rohu (Labeo rohita), a commercially important freshwater fish species in the Indian subcontinent. The rohu IFN-ãrel gene consisted of four exons with three intervening introns and phylogenetically closely related to grass carp. The full-length IFN-ãrel cDNA comprised 927 bp nucleotides with a single open reading frame of 504 bp, encoding 167 amino acids (aa) polypeptide with a signal peptide of 24 aa. The mature rohu IFN-ãrel protein was 143 aa with a predicted molecular weight of 16.85 kDa. Basal expression analysis of IFN-ãrel showed its wide range of expression in all examined tissues: The highest was in the skin and the lowest was in the liver. In response to LPS, poly I:C, iE-DAP, muramyl dipeptide stimulations, and bacterial infections, IFN-ãrel gene expression was significantly (p<0.05) induced in treated fish tissues as compared with their control. The IFN-ãrel was expressed as recombinant protein (rIFN-ãrel) and confirmed through western blot. Stimulation of peripheral blood leukocytes with rIFN-ãrel protein resulted in the activation of IFN-ã receptor and marked induction of inducible nitric oxide synthase gene expression. These results together may suggest the important role of IFN-ãrel as an antimicrobial cytokine in fish.
|
['Amino Acid Sequence', 'Animals', 'Bacteria', 'Bacterial Infections', 'Base Sequence', 'Blotting, Western', 'Carps', 'Cloning, Molecular', 'DNA, Complementary', 'Fish Diseases', 'Fish Proteins', 'Gene Expression Profiling', 'Gene Expression Regulation', 'Interferon-gamma', 'Lipopolysaccharides', 'Molecular Sequence Data', 'RNA, Messenger', 'Real-Time Polymerase Chain Reaction', 'Receptors, Interferon', 'Reverse Transcriptase Polymerase Chain Reaction', 'Sequence Homology, Amino Acid', 'Tumor Cells, Cultured']
| 25,756,860
|
[['G02.111.570.060', 'L01.453.245.667.060'], ['B01.050'], ['B03'], ['C01.150.252'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['E05.196.401.143', 'E05.301.300.096', 'E05.478.566.320.200', 'E05.601.262', 'E05.601.470.320.200'], ['B01.050.150.900.493.200.244.248'], ['E05.393.220'], ['D13.444.308.497.220', 'D13.444.600.223.500', 'D27.720.470.530.600.223.260'], ['C22.362'], ['D12.776.325'], ['E05.393.332'], ['G05.308'], ['D12.644.276.374.440.893', 'D12.644.276.374.480.615.350', 'D12.776.467.374.440.893', 'D12.776.467.374.480.615.350', 'D23.529.374.440.893', 'D23.529.374.480.615.350'], ['D09.400.500', 'D09.698.718.450', 'D10.494', 'D23.050.161.616.525', 'D23.946.123.329.500'], ['L01.453.245.667'], ['D13.444.735.544'], ['E05.393.620.500.706'], ['D12.776.543.750.705.852.400'], ['E05.393.620.500.725'], ['G02.111.810.200', 'G05.810.200'], ['A11.251.860']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Organisms [B]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
HLA-DPB polymorphisms: Glu 69 association with sarcoidosis.
|
Sarcoidosis is a chronic granulomatous disorder, which is characterized by the accumulation of activated CD4+ T lymphocytes (T cells) at disease sites. There is up-regulation of cell surface expression of MHC molecules in sarcoidosis, and it has been suggested that specific MHC class II alleles are associated with the disease. A study of chronic beryllium disease (CBD), a granulomatous disorder which is pathologically similar to sarcoidosis, has identified an association between this disease and the presence of a glutamine residue at position 69 (Glu 69+) of the B1 chain of the HLA-DPB molecule. A further study also suggested the importance of Glu at position 55 of the same chain. The aims of the present study were to attempt to define MHC class II alleles associated with sarcoidosis by comparison of their frequency in two groups of subjects and to compare the frequency of HLA-DPB1 Glu 69+/- and Glu 55+/-alleles in the same subjects. Forty-one subjects with sarcoidosis and 76 normal subjects were studied. The polymorphic regions of the class II MHC were identified by PCR in association with sequence-specific oligonucleotide probes. There were no significant differences in the phenotype frequencies of MHC class II or Glu 55+ alleles between the two groups of subjects. However, there was a significant increase (P = 0.02) in the frequency of HLA-DPB1* Glu 69+ alleles compared with the control population. We therefore suggest that the presence of a Glu residue at position 69 on the DPB1 chain may play an important role in antigen presentation and recognition in chronic granulomatous diseases.
|
['Alleles', 'Gene Frequency', 'HLA-DP Antigens', 'HLA-DP beta-Chains', 'HLA-DQ Antigens', 'HLA-DQ alpha-Chains', 'HLA-DQ beta-Chains', 'HLA-DR Antigens', 'HLA-DRB1 Chains', 'Humans', 'Phenotype', 'Polymorphism, Genetic', 'Sarcoidosis']
| 8,909,942
|
[['G05.360.340.024.340.030'], ['G05.330'], ['D12.776.395.550.509.400.420', 'D12.776.543.550.440.400.420', 'D23.050.301.500.400.400.420', 'D23.050.301.500.450.400.420', 'D23.050.705.552.410.400.420', 'D23.050.705.552.450.400.420'], ['D12.776.395.550.509.400.420.750', 'D12.776.543.550.440.400.420.750', 'D23.050.301.500.400.400.420.750', 'D23.050.301.500.450.400.420.750', 'D23.050.705.552.410.400.420.750', 'D23.050.705.552.450.400.420.750'], ['D12.776.395.550.509.400.430', 'D12.776.543.550.440.400.430', 'D23.050.301.500.400.400.430', 'D23.050.301.500.450.400.430', 'D23.050.705.552.410.400.430', 'D23.050.705.552.450.400.430'], ['D12.776.395.550.509.400.430.500', 'D12.776.543.550.440.400.430.500', 'D23.050.301.500.400.400.430.500', 'D23.050.301.500.450.400.430.500', 'D23.050.705.552.410.400.430.500', 'D23.050.705.552.450.400.430.500'], ['D12.776.395.550.509.400.430.750', 'D12.776.543.550.440.400.430.750', 'D23.050.301.500.400.400.430.750', 'D23.050.301.500.450.400.430.750', 'D23.050.705.552.410.400.430.750', 'D23.050.705.552.450.400.430.750'], ['D12.776.395.550.509.400.440', 'D12.776.543.550.440.400.440', 'D23.050.301.500.400.400.440', 'D23.050.301.500.450.400.440', 'D23.050.705.552.410.400.440', 'D23.050.705.552.450.400.440'], ['D12.776.395.550.509.400.440.200.010', 'D12.776.543.550.440.400.440.200.010', 'D23.050.301.500.400.400.440.200.010', 'D23.050.301.500.450.400.440.333.500', 'D23.050.705.552.410.400.440.200.010', 'D23.050.705.552.450.400.440.333.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G05.695'], ['G05.365.795'], ['C15.604.515.827']]
|
['Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Differential response of the human hepatoma-derived cell line HA22T/VGH to polypeptide mitogens.
|
Several human cell lines derived from primary cancer of the liver are able to grow under serum-free conditions and produce spreading and growth factors which are released into the culture medium. Since this autocrine growth under hormone-free conditions might play a basic role in malignant transformation, we studied the effect on cell replication and the presence of specific membrane receptors of epidermal growth factor (EGF) and insulin on a dedifferentiated human hepatoma cell line, named HA22T/VGH. Our results point to a similar inhibitory effect on cell replication in the presence of both EGF and insulin, in spite of detecting different affinities of binding.
|
['Carcinoma, Hepatocellular', 'Cell Division', 'Cell Line', 'DNA, Viral', 'Epidermal Growth Factor', 'ErbB Receptors', 'Growth Hormone', 'Hepatitis B Surface Antigens', 'Hepatitis B virus', 'Humans', 'Insulin', 'Liver Neoplasms', 'Receptor, Insulin']
| 3,040,466
|
[['C04.557.470.200.025.255', 'C04.588.274.623.160', 'C06.301.623.160', 'C06.552.697.160'], ['G04.144.220', 'G04.161.750.500', 'G05.113', 'G07.345.249.410.750.500'], ['A11.251.210'], ['D13.444.308.568'], ['D06.472.317.350', 'D12.644.276.382.500', 'D12.776.467.382.500', 'D23.529.382.500'], ['D08.811.913.696.620.682.725.400.009', 'D12.776.543.750.630.009', 'D12.776.543.750.750.400.074'], ['D06.472.699.631.525.425', 'D12.644.548.691.525.425'], ['D23.050.327.495.500.475'], ['B04.280.375.650.425', 'B04.450.390.650.425'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D06.472.699.587.200.500.625', 'D12.644.548.586.200.500.625'], ['C04.588.274.623', 'C06.301.623', 'C06.552.697'], ['D08.811.913.696.620.682.725.400.200', 'D12.776.543.750.630.484', 'D12.776.543.750.750.580.300']]
|
['Diseases [C]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Organisms [B]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Two new species of Oswaldocruzia (Nematoda: Trichostrongylina: Molineoidea) parasites of the cane toad Rhinella marina (Amphibia: Anura) from Peru.
|
Two new species of Oswaldocruzia, O. manuensis sp. nov., and O. urubambaensis sp. nov. are described and illustrated from Peru, these are parasites of the cane toad Rhinella marina. O. manuensis is characterized by having cervical alae which are not well developed, ridges without chitinous supports, caudal bursa type II and branches of fork of dissimilar length. O. urubambaensis is characterized by a caudal bursa of type I, ridges with chitinous supports, a thin cephalic vesicle and origin of rays 9 in tip of the dorsal trunk.
|
['Animals', 'Anura', 'Female', 'Male', 'Nematoda', 'Nematode Infections', 'Peru']
| 23,377,910
|
[['B01.050'], ['B01.050.150.900.090.180'], ['B01.050.500.500.294'], ['C01.610.335.508'], ['Z01.107.757.702']]
|
['Organisms [B]', 'Diseases [C]', 'Geographicals [Z]']
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
|
'Hyperactivity' in childhood.
|
The management of a 'hyperactive' child entails a thorough individual assessment, close communication with the school, intensive work with the family and the development of an individualised management plan which takes account of the unique circumstances of the child and his or her environment.
|
['Attention Deficit Disorder with Hyperactivity', 'Child', 'Humans']
| 6,639,482
|
[['F03.625.094.150'], ['M01.060.406'], ['B01.050.150.900.649.313.988.400.112.400.400']]
|
['Psychiatry and Psychology [F]', 'Named Groups [M]', 'Organisms [B]']
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
OC-STAMP promotes osteoclast fusion for pathogenic bone resorption in periodontitis via up-regulation of permissive fusogen CD9.
|
Cell fusion-mediated formation of multinuclear osteoclasts (OCs) plays a key role in bone resorption. It is reported that 2 unique OC-specific fusogens [ i.e., OC-stimulatory transmembrane protein (OC-STAMP) and dendritic cell-specific transmembrane protein (DC-STAMP)], and permissive fusogen CD9, are involved in OC fusion. In contrast to DC-STAMP-knockout (KO) mice, which show the osteopetrotic phenotype, OC-STAMP-KO mice show no difference in systemic bone mineral density. Nonetheless, according to the ligature-induced periodontitis model, significantly lower level of bone resorption was found in OC-STAMP-KO mice compared to WT mice. Anti-OC-STAMP-neutralizing mAb down-modulated in vitro: 1) the emergence of large multinuclear tartrate-resistant acid phosphatase-positive cells, 2) pit formation, and 3) mRNA and protein expression of CD9, but not DC-STAMP, in receptor activator of NF-êB ligand (RANKL)-stimulated OC precursor cells (OCps). While anti-DC-STAMP-mAb also down-regulated RANKL-induced osteoclastogenesis in vitro, it had no effect on CD9 expression. In our mouse model, systemic administration of anti-OC-STAMP-mAb suppressed the expression of CD9 mRNA, but not DC-STAMP mRNA, in periodontal tissue, along with diminished alveolar bone loss and reduced emergence of CD9+ OCps and tartrate-resistant acid phosphatase-positive multinuclear OCs. The present study demonstrated that OC-STAMP partners CD9 to promote periodontal bone destruction by up-regulation of fusion during osteoclastogenesis, suggesting that anti-OC-STAMP-mAb may lead to the development of a novel therapeutic regimen for periodontitis.-Ishii, T., Ruiz-Torruella, M., Ikeda, A., Shindo, S., Movila, A., Mawardi, H., Albassam, A., Kayal, R. A., Al-Dharrab, A. A., Egashira, K., Wisitrasameewong, W., Yamamoto, K., Mira, A. I., Sueishi, K., Han, X., Taubman, M. A., Miyamoto, T., Kawai, T. OC-STAMP promotes osteoclast fusion for pathogenic bone resorption in periodontitis via up-regulation of permissive fusogen CD9.
|
['Alveolar Bone Loss', 'Animals', 'Antibodies, Neutralizing', 'Cells, Cultured', 'Male', 'Membrane Proteins', 'Mice', 'Mice, Inbred C57BL', 'Osteoclasts', 'Tetraspanin 29', 'Up-Regulation']
| 29,533,736
|
[['C05.116.264.150', 'C07.465.714.354.500'], ['B01.050'], ['D12.776.124.486.485.114.244', 'D12.776.124.790.651.114.244', 'D12.776.377.715.548.114.244'], ['A11.251'], ['D12.776.543'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.420', 'B01.050.150.900.649.313.992.635.505.500.400.420'], ['A11.329.372.700', 'A11.627.482.700'], ['D12.776.395.550.018', 'D12.776.543.550.090', 'D12.776.543.900.109'], ['G02.111.905', 'G05.308.850', 'G07.690.773.998']]
|
['Diseases [C]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[Botulism poisoning in cattle, a case report, diagnosis and prevention].
|
On a dairy farm 22 animals die in 14 days. After 10 days the clinical diagnosis is confirmed: clostridium botulinum type D intoxication. The clinical, diagnosis, therapy and prevention are discussed.
|
['Animals', 'Bacterial Vaccines', 'Botulinum Toxins', 'Botulism', 'Cattle', 'Cattle Diseases', 'Clostridium botulinum', 'Dairying', 'Diagnosis, Differential', 'Disease Outbreaks', 'Female', 'Food Contamination', 'Netherlands']
| 12,125,164
|
[['B01.050'], ['D20.215.894.135'], ['D08.811.277.656.300.480.153', 'D08.811.277.656.675.374.153', 'D12.776.097.156', 'D23.946.123.179'], ['C01.150.252.410.222.151', 'C10.668.758.200', 'C10.720.150', 'C25.723.415.151'], ['B01.050.150.900.649.313.500.380.271'], ['C22.196'], ['B03.300.390.400.200.160', 'B03.353.625.375.500.160', 'B03.510.415.400.200.160'], ['J01.040.246'], ['E01.171'], ['N06.850.290'], ['J01.576.423.850.730.500.249', 'N06.850.460.400', 'N06.850.601.500.249'], ['Z01.542.651']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Technology, Industry, and Agriculture [J]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Geographicals [Z]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 1
|
Radiosynthesis and biodistribution of 123I-labeled antagonists of the histamine H3 receptor as potential SPECT ligands.
|
We have synthesized three 123I-labeled histamine H3 receptor ligands, i.e., [123I]GR 190028, [123I]FUB 271, and [123I]iodoproxyfan, in moderate to good radiochemical yields via a Cu+-assisted I-for-123I exchange method. Biodistribution in the rat of these compounds revealed high hepatic and pulmonary uptake. Brain uptake was moderate, but for [123I]iodoproxyfan, brain uptake was high enough for a pilot single photon emission computed tomography (SPECT) study in the rabbit. However, for this compound, the cerebral uptake could not be blocked by a pretreatment with [R]-alpha-methylhistamine, a selective, high-affinity histamine H3 receptor agonist, both in the SPECT study in the rabbit and in the biodistribution study in the rat. Apparently, [123I]iodoproxyfan is binding to a non-H3 receptor binding site. None of the three investigated compounds is suitable for use as a SPECT ligand for the H3 receptor in the brain.
|
['Animals', 'Brain', 'Cerebellum', 'Cerebral Cortex', 'Corpus Striatum', 'Histamine Antagonists', 'Imidazoles', 'Iodine Radioisotopes', 'Iodobenzenes', 'Male', 'Oxadiazoles', 'Rabbits', 'Radioligand Assay', 'Rats', 'Rats, Wistar', 'Receptors, Histamine H3', 'Tissue Distribution', 'Tomography, Emission-Computed, Single-Photon']
| 10,587,103
|
[['B01.050'], ['A08.186.211'], ['A08.186.211.132.810.428.200'], ['A08.186.211.200.885.287.500'], ['A08.186.211.200.885.287.249.487'], ['D27.505.519.625.375.425', 'D27.505.696.577.375.425'], ['D03.383.129.308'], ['D01.268.380.400.500.496', 'D01.496.448.496', 'D01.496.749.474'], ['D02.455.426.559.389.454', 'D02.455.526.581.496'], ['D03.383.129.462.580'], ['B01.050.150.900.649.313.968.700'], ['E01.370.225.985', 'E01.370.374.650', 'E01.370.384.720', 'E05.200.985'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.900'], ['D12.776.543.750.670.450.500', 'D12.776.543.750.720.480.500'], ['G03.787.917', 'G07.690.725.949'], ['E01.370.350.350.800.800', 'E01.370.350.600.350.800.800', 'E01.370.350.710.800.800', 'E01.370.350.825.800.800', 'E01.370.384.730.800.800']]
|
['Organisms [B]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Genotoxic evaluation of the furylethylene derivative 2-furyl-1-nitroethene in cultured human lymphocytes.
|
The compound 2-furyl-1-nitroethene (G-0) was evaluated for genotoxicity in cultured human peripheral blood lymphocytes, at concentrations ranging from 1 to 15microg/ml. Micronuclei (MN) and sister-chromatid exchanges (SCEs) were scored as genetic endpoints. In order to detect the role of metabolic enzymes on the genotoxicity of this furylethylenic derivative, the cultures for MN and SCE demonstrations were also treated with S9 microsomal fraction. The results indicate that, under the conditions of the study, the test agent does not seem to induce significant increases in the frequency of micronucleated cells, irrespective of the presence of metabolic activation. Nevertheless, a slight increase in the SCE frequency was observed in those cultures treated without the S9 mix; although this increase disappeared in presence of the microsomal fraction. In addition, cytostatic effects of 2-furyl-1-nitroethene were observed mainly in cultures without S9 fraction, as indicated by the reduction of cell proliferation.
|
['Animals', 'Biotransformation', 'Cells, Cultured', 'Furans', 'Humans', 'In Vitro Techniques', 'Lymphocytes', 'Micronucleus Tests', 'Microsomes, Liver', 'Mutagens', 'Nitro Compounds', 'Sister Chromatid Exchange']
| 11,525,921
|
[['B01.050'], ['G03.171', 'G03.787.225', 'G07.690.725.225'], ['A11.251'], ['D03.383.312'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.481'], ['A11.118.637.555.567', 'A15.145.229.637.555.567', 'A15.382.490.555.567'], ['E05.393.560.598'], ['A11.284.835.540.541'], ['D27.888.569.468'], ['D02.640'], ['G05.728.615.750']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
PCR primers specific for the genus Tuber reveal the presence of several truffle species in a truffle-ground.
|
Truffles are hypogeous Ascomycete fungi belonging to the genus Tuber and forming fruiting bodies highly prized for their taste and aroma. The identification of the genus Tuber and its species is important to investigate their ecology and avoid fraud in the food market. As genus-specific primers are not available, the aims of this work were (1) to assess the usefulness of the beta-tubulin gene as a DNA barcoding region for designing Tuber genus-specific primers, (2) to test the primers on a range of fruiting bodies, representing a large part of truffle biodiversity and (3) to check their ecological usefulness, applying them to truffle-ground soil. The new primers designed on the beta-tubulin gene were specific to the Tuber genus in nested PCR. When applied to DNA from soils, they gave a positive signal for 23 of 32 soils. Phylogenetic analysis confirmed that the bands corresponded to Tuber and that at least five Tuber species were present in the truffle-ground. beta-tubulin was found to be a good barcoding region for designing Tuber genus-specific primers, detecting a high Tuber diversity in a natural environment. These primers will be useful for understanding truffle ecology and for practical needs in plantation management.
|
['Ascomycota', 'DNA Primers', 'Fungal Proteins', 'Molecular Sequence Data', 'Mycological Typing Techniques', 'Phylogeny', 'Polymerase Chain Reaction', 'Soil Microbiology', 'Species Specificity', 'Tubulin']
| 19,519,770
|
[['B01.300.107'], ['D13.695.578.424.450.275', 'D27.720.470.530.600.223.600'], ['D12.776.354'], ['L01.453.245.667'], ['E01.370.225.875.610', 'E05.200.875.610'], ['G05.697', 'G16.075.605', 'L01.100.697'], ['E05.393.620.500'], ['H01.158.273.540.274.555', 'N06.850.425.300'], ['G16.824'], ['D05.750.078.734.800', 'D12.776.220.600.800', 'D12.776.631.920']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Disciplines and Occupations [H]', 'Health Care [N]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 0
|
A high-performance liquid chromatography procedure for recovering subnanomole amounts of protein from SDS-gel electroeluates for gas-phase sequence analysis.
|
A high-performance liquid chromatographic procedure for recovering subnanomole amounts of protein from SDS/polyacrylamide gel electroeluates in a form suitable for gas-phase sequence analysis has been developed. By a judicious choice of reversed-phase column packing, proteins can be retained at high concentrations of n-propanol (90-100%) where sodium dodecylsulfate and acrylamide gel-related contaminants are washed through the column. Retained proteins can be recovered from the column in high yield (greater than 90%) by the simultaneous adding of an ion-pairing reagent into the mobile phase and elution with a gradient of decreasing n-propanol concentration (i.e. an 'inverse or negative gradient'). Furthermore, by using a steep gradient (e.g. 50%/min) at a low flow rate (20-200 microliters/min) the proteins can be recovered in less than 100 microliters and can be used for gas-phase sequence analysis without further manipulation. This procedure is independent of sodium dodecylsulfate concentration (up to 1.2% w/v) in sample loading volumes of up to 1.5 ml. Microbore columns (2.1 mm internal diameter) have been employed for recovering small amounts of protein (1-100 micrograms from electroeluates of protein-containing gel spots while conventional columns (4.6 mm internal diameter) were used for isolating larger amounts of protein (greater than 500 micrograms) from electroeluates of preparative gel bands. The general utility of this inverse-gradient high-performance liquid chromatography procedure has been demonstrated by its successful application in recovering a wide variety of proteins from sodium dodecylsulfate gel electroeluates in a form suitable for N-terminal sequence analysis in the 10-500 pmol range.
|
['Chromatography, High Pressure Liquid', 'Electrophoresis, Polyacrylamide Gel', 'Indicators and Reagents', 'Microchemistry', 'Proteins', 'Structure-Activity Relationship']
| 3,569,294
|
[['E05.196.181.400.300'], ['E05.196.401.402', 'E05.301.300.319'], ['D27.720.470.410'], ['E05.196.620', 'H01.181.650'], ['D12.776'], ['G02.111.830', 'G07.690.773.997']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Disciplines and Occupations [H]', 'Phenomena and Processes [G]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
Influence of dental materials on dental MRI.
|
OBJECTIVES: To investigate the potential influence of standard dental materials on dental MRI (dMRI) by estimating the magnetic susceptibility with the help of the MRI-based geometric distortion method and to classify the materials from the standpoint of dMRI.METHODS: A series of standard dental materials was studied on a 1.5 T MRI system using spin echo and gradient echo pulse sequences and their magnetic susceptibility was estimated using the geometric method. Measurements on samples of dental materials were supported by in vivo examples obtained in dedicated dMRI procedures.RESULTS: The tested materials showed a range of distortion degrees. The following materials were classified as fully compatible materials that can be present even in the tooth of interest: the resin-based sealer AH Plus(®) (Dentsply, Maillefer, Germany), glass ionomer cement, gutta-percha, zirconium dioxide and composites from one of the tested manufacturers. Interestingly, composites provided by the other manufacturer caused relatively strong distortions and were therefore classified as compatible I, along with amalgam, gold alloy, gold-ceramic crowns, titanium alloy and NiTi orthodontic wires. Materials, the magnetic susceptibility of which differed from that of water by more than 200 ppm, were classified as non-compatible materials that should not be present in the patient's mouth for any dMRI applications. They included stainless steel orthodontic appliances and CoCr.CONCLUSIONS: A classification of the materials that complies with the standard grouping of materials according to their magnetic susceptibility was proposed and adopted for the purposes of dMRI. The proposed classification can serve as a guideline in future dMRI research.
|
['Artifacts', 'Chromium Alloys', 'Composite Resins', 'Crowns', 'Dental Alloys', 'Dental Amalgam', 'Dental Materials', 'Epoxy Resins', 'Glass Ionomer Cements', 'Gold Alloys', 'Gutta-Percha', 'Humans', 'Image Enhancement', 'Magnetic Resonance Imaging', 'Magnetics', 'Metal Ceramic Alloys', 'Nickel', 'Orthodontic Brackets', 'Orthodontic Wires', 'Root Canal Filling Materials', 'Stainless Steel', 'Titanium', 'Zirconium']
| 23,610,088
|
[['E05.047'], ['D01.220.175', 'D01.552.033.182', 'D25.058.224', 'D25.339.208.224', 'J01.637.051.058.224', 'J01.637.051.339.208.224'], ['D05.750.716.822.308', 'D25.339.816.500', 'D25.720.716.822.308', 'J01.637.051.339.816.500', 'J01.637.051.720.716.822.308'], ['E06.780.346.250', 'E07.695.190.088'], ['D25.339.208', 'J01.637.051.339.208'], ['D25.339.208.291', 'J01.637.051.339.208.291'], ['D25.339', 'D27.720.102.339', 'J01.637.051.339'], ['D05.750.716.822.461', 'D25.720.716.822.461', 'J01.637.051.720.716.822.461'], ['D25.339.291.402', 'J01.637.051.339.291.402'], ['D01.379.375', 'D01.552.033.533', 'D25.058.451', 'D25.339.208.534', 'J01.637.051.058.451', 'J01.637.051.339.208.534'], ['D20.215.721.061', 'D25.339.859.495', 'D25.720.327.840.119', 'J01.637.051.339.859.495'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.350.600.350', 'L01.224.308.380'], ['E01.370.350.825.500'], ['H01.671.493'], ['D01.552.033.690', 'D25.058.520', 'D25.339.208.720', 'J01.637.051.058.520', 'J01.637.051.339.208.720'], ['D01.268.556.607', 'D01.268.956.625', 'D01.552.544.607'], ['E06.658.453.255.500'], ['E06.658.453.684'], ['D25.339.859', 'J01.637.051.339.859'], ['D01.490.800.900', 'D01.552.033.847.681', 'D25.058.807.681', 'J01.637.051.058.807.681'], ['D01.268.557.800', 'D01.268.956.878', 'D01.552.547.800'], ['D01.268.556.950', 'D01.268.956.937', 'D01.552.544.950']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Technology, Industry, and Agriculture [J]', 'Organisms [B]', 'Information Science [L]', 'Disciplines and Occupations [H]']
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 0
|
Cancer patient pathways in Denmark as a joint effort between bureaucrats, health professionals and politicians--a national Danish project.
|
In 2007 and 2008 Danish Cancer Patient Pathways for 32 cancer types were developed and afterwards implemented on a national scale. Often bureaucrats, health professionals and politicians look upon the health sector in different ways and work independent of each other. In Denmark, as indeed internationally, patient pathways are frequently developed solely by health professionals and the consequence may be major difficulties in implementing the pathways on a national scale. In this article we describe how national Danish Cancer Patient Pathways were developed with a consensus seeking model and the impact it has had on the health system. The model used in Denmark ensured involvement and cooperation between bureaucrats, health professionals and politicians and afterwards a successful national implementation. The Cancer Patient Pathways has significantly reduced waiting times which is thought to increase survival. This experience gives important input to the continuous challenges on how to implement evidence based medicine on a national scale and stipulates a model for this process.
|
['Cooperative Behavior', 'Critical Pathways', 'Denmark', 'Government Agencies', 'Health Personnel', 'Humans', 'Models, Organizational', 'National Health Programs', 'Neoplasms', 'Politics', 'Program Development', 'Program Evaluation', 'Waiting Lists']
| 22,136,810
|
[['F01.145.813.115'], ['N04.590.233.624.625', 'N04.590.275'], ['Z01.542.816.124'], ['I01.409.418', 'N03.540.400'], ['M01.526.485', 'N02.360'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.599.670', 'N04.452.534'], ['N03.349.550'], ['C04'], ['I01.738'], ['N04.452.760'], ['E05.337.820', 'N04.761.685', 'N05.715.360.650'], ['N04.452.095.738']]
|
['Psychiatry and Psychology [F]', 'Health Care [N]', 'Geographicals [Z]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Named Groups [M]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]']
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 1
|
Association between expression of embryonic lethal abnormal vision-like protein HuR and cyclooxygenase-2 in oral squamous cell carcinoma.
|
BACKGROUND: Increased cytoplasmic HuR expression has been noted in several cancer types, where it may contribute to the increased cyclooxygenase-2 (COX-2) expression observed during tumorigenesis and metastasis.METHODS: To assess the correlation between COX-2 and HuR in oral squamous cell carcinoma (OSCC), the expression patterns of HuR and COX-2 were assessed via immunohistochemistry analyses of 103 OSCC samples.RESULTS: Cytoplasmic HuR expression was significantly associated with COX-2 expression (p < .025) and lymph node metastasis (p < .050) and distant metastasis (p < .025). In multivariate analysis, cytoplasmic HuR expression was identified as an independent prognostic parameter for reduced overall survival. The inhibition of HuR expression by siRNA or leptomycin B (LMB) caused a reduction in the inducibility of COX-2 in oral cancer cells.CONCLUSION: Our results indicate that the cytoplasmic expression of HuR is associated with COX-2 expression in OSCCs and HuR can regulate COX-2 expression in oral cancer cell lines.
|
['Antigens, Surface', 'Blotting, Western', 'Carcinoma, Squamous Cell', 'Cell Line, Tumor', 'Cyclooxygenase 2', 'Cytoplasm', 'ELAV Proteins', 'ELAV-Like Protein 1', 'Fatty Acids, Unsaturated', 'Female', 'Humans', 'Immunohistochemistry', 'Lymphatic Metastasis', 'Male', 'Middle Aged', 'Mouth Neoplasms', 'Multivariate Analysis', 'Prognosis', 'RNA, Messenger', 'RNA, Small Interfering', 'RNA-Binding Proteins', 'Sex Factors', 'Tumor Cells, Cultured']
| 20,960,565
|
[['D23.050.301'], ['E05.196.401.143', 'E05.301.300.096', 'E05.478.566.320.200', 'E05.601.262', 'E05.601.470.320.200'], ['C04.557.470.200.400', 'C04.557.470.700.400'], ['A11.251.210.190', 'A11.251.860.180'], ['D08.811.600.720.750'], ['A11.284.430.214'], ['D12.776.157.725.813.500', 'D12.776.631.520', 'D12.776.664.962.813.500'], ['D12.776.157.725.813.500.250', 'D12.776.664.962.813.500.250'], ['D10.251.355'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.225.500.607.512', 'E01.370.225.750.551.512', 'E05.200.500.607.512', 'E05.200.750.551.512', 'E05.478.583', 'H01.158.100.656.234.512', 'H01.158.201.344.512', 'H01.158.201.486.512', 'H01.181.122.573.512', 'H01.181.122.605.512'], ['C04.697.650.560', 'C23.550.727.650.560'], ['M01.060.116.630'], ['C04.588.443.591', 'C07.465.530'], ['E05.318.740.150.500', 'N05.715.360.750.125.500', 'N06.850.520.830.150.500'], ['E01.789'], ['D13.444.735.544'], ['D13.150.650.700', 'D13.444.735.150.700', 'D13.444.735.790.552.875'], ['D12.776.157.725', 'D12.776.664.962'], ['N05.715.350.675', 'N06.850.490.875'], ['A11.251.860']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Anatomy [A]', 'Organisms [B]', 'Disciplines and Occupations [H]', 'Named Groups [M]', 'Health Care [N]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 1
| 0
|
Photopolymerized thermosensitive poly(HPMAlactate)-PEG-based hydrogels: effect of network design on mechanical properties, degradation, and release behavior.
|
Photopolymerized thermosensitive A-B-A triblock copolymer hydrogels composed of poly(N-(2-hydroxypropyl)methacrylamide lactate) A-blocks, partly derivatized with methacrylate groups to different extents (10, 20, and 30%) and hydrophilic poly(ethylene glycol) B-blocks of different molecular weights (4, 10, and 20 kDa) were synthesized. The aim of the present study was to correlate the polymer architecture with the hydrogel properties, particularly rheological, swelling, degradation properties and release behavior. It was found that an increasing methacrylation extent and a decreasing PEG molecular weight resulted in increasing gel strength and cross-link density, which tailored the degradation profiles from 25 to more than 300 days. Polymers having small PEG blocks showed a remarkable phase separation into polymer- and water-rich domains, as demonstrated by confocal microscopy. Depending on the hydrophobic domain density, the loaded protein resides in the hydrophilic pores or is partitioned into hydrophilic and hydrophobic domains, and its release from these compartments is tailored by the extent of methacrylation and by PEG length, respectively. As the mechanical properties, degradation, and release profiles can be fully controlled by polymer design and concentration, these hydrogels are suitable for controlled protein release.
|
['Acrylamides', 'Drug Delivery Systems', 'Hydrogels', 'Magnetic Resonance Spectroscopy', 'Microscopy, Confocal', 'Molecular Weight', 'Polyethylene Glycols', 'Rheology', 'Serum Albumin, Bovine']
| 20,614,933
|
[['D02.065.122', 'D02.241.081.069.094'], ['E02.319.300'], ['D20.280.320.375', 'D26.255.165.320.375'], ['E05.196.867.519'], ['E01.370.350.515.395', 'E05.595.395'], ['G02.494'], ['D02.033.455.250.700', 'D05.750.741', 'D25.720.741', 'J01.637.051.720.741'], ['E05.830', 'H01.671.808'], ['D12.776.034.841.540', 'D12.776.124.727.875']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Technology, Industry, and Agriculture [J]', 'Disciplines and Occupations [H]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
|
Induction of the mesendoderm in the zebrafish germ ring by yolk cell-derived TGF-beta family signals and discrimination of mesoderm and endoderm by FGF.
|
The endoderm forms the gut and associated organs, and develops from a layer of cells which emerges during gastrula stages in the vertebrate embryo. In comparison to mesoderm and ectoderm, little is known about the signals which induce the endoderm. The origin of the endoderm is intimately linked with that of mesoderm, both by their position in the embryo, and by the molecules that can induce them. We characterised a gene, zebrafish gata5, which is expressed in the endoderm from blastula stages and show that its transcription is induced by signals originating from the yolk cell. These signals also induce the mesoderm-expressed transcription factor no tail (ntl), whose initial expression coincides with gata5 in the cells closest to the blastoderm margin, then spreads to encompass the germ ring. We have characterised the induction of these genes and show that ectopic expression of activin induces gata5 and ntl in a pattern which mimics the endogenous expression, while expression of a dominant negative activin receptor abolishes ntl and gata5 expression. Injection of RNA encoding a constitutively active activin receptor leads to ectopic expression of gata5 and ntl. gata5 is activated cell-autonomously, whereas ntl is induced in cells distant from those which have received the RNA, showing that although expression of both genes is induced by a TGF-beta signal, expression of ntl then spreads by a relay mechanism. Expression of a fibroblast growth factor (eFGF) or a dominant negatively acting FGF receptor shows that ntl but not gata5 is regulated by FGF signalling, implying that this may be the relay signal leading to the spread of ntl expression. In embryos lacking both squint and cyclops, members of the nodal group of TGF-beta related molecules, gata5 expression in the blastoderm is abolished, making these factors primary candidates for the endogenous TGF-beta signal inducing gata5.
|
['Activins', 'Amino Acid Sequence', 'Animals', 'Base Sequence', 'Blastoderm', 'Cloning, Molecular', 'DNA-Binding Proteins', 'Digestive System', 'Embryonic Induction', 'Endoderm', 'Fetal Proteins', 'Fibroblast Growth Factors', 'GATA5 Transcription Factor', 'Gastrula', 'Gene Expression Regulation, Developmental', 'Germ Cells', 'Heart', 'Inhibins', 'Mesoderm', 'Molecular Sequence Data', 'Myocardium', 'Oligopeptides', 'Peptides', 'Polymerase Chain Reaction', 'Sequence Homology, Amino Acid', 'Signal Transduction', 'T-Box Domain Proteins', 'Tail', 'Transcription Factors', 'Transforming Growth Factor beta', 'Zebrafish', 'Zebrafish Proteins']
| 10,375,499
|
[['D06.472.334.500', 'D06.472.699.009', 'D12.644.548.009', 'D12.776.395.022'], ['G02.111.570.060', 'L01.453.245.667.060'], ['B01.050'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['A16.331.024'], ['E05.393.220'], ['D12.776.260'], ['A03'], ['G04.085.300', 'G04.152.300', 'G07.345.500.100.250', 'G07.345.500.325.180.750', 'G08.686.784.170.104.750'], ['A16.504.407'], ['D12.776.320'], ['D12.644.276.624', 'D12.776.467.624', 'D23.529.624'], ['D12.776.260.257.500', 'D12.776.930.314.500'], ['A16.441'], ['G05.308.310'], ['A05.360.490', 'A11.497'], ['A07.541'], ['D06.472.334.968', 'D06.472.699.337', 'D12.644.548.387', 'D12.776.395.439'], ['A16.504.660'], ['L01.453.245.667'], ['A02.633.580', 'A07.541.704', 'A10.690.552.750'], ['D12.644.456'], ['D12.644'], ['E05.393.620.500'], ['G02.111.810.200', 'G05.810.200'], ['G02.111.820', 'G04.835'], ['D12.776.260.725', 'D12.776.930.850'], ['A13.895'], ['D12.776.930'], ['D12.644.276.374.687', 'D12.644.276.954.775', 'D12.776.467.374.687', 'D12.776.467.942.775', 'D23.529.374.687', 'D23.529.942.775'], ['B01.050.150.900.493.200.244.828'], ['D12.776.325.500']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Organisms [B]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
A palladium(II) triangle as building blocks of microporous molecular materials: structures and catalytic performance.
|
A porous molecular crystalline solid based on amide-containing Pd(II) triangles was created for size-selective heterogeneous catalysis of the Knoevenagel condensation reaction.
|
['Aldehydes', 'Amides', 'Catalysis', 'Ethers', 'Molecular Structure', 'Nitriles', 'Organometallic Compounds', 'Palladium', 'Particle Size', 'Porosity', 'Surface Properties']
| 20,087,507
|
[['D02.047'], ['D02.065'], ['G02.130'], ['D02.355'], ['G02.111.570', 'G02.466'], ['D02.626'], ['D02.691'], ['D01.268.556.680', 'D01.268.956.718', 'D01.552.544.680'], ['G02.712'], ['G01.374.710'], ['G02.860']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]']
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Tumor host relations. I. Increased plasma cortisol in tumor-bearing humans compared with patients with benign surgical diseases.
|
Plasma cortisol was measured by a protein binding technique in 81 patients with malignant tumors of different extent and various sites and in 82 patients with benign surgical diseases. The mean value of the tumor patients (x +/- s = 165 +/- 69 micrograms cortisol/l plasma) was increased significantly compared with the benign surgical disorders (100 +/- 45 micrograms/l). Within the group of patients with benign surgical disorders there was little variation by the type of disease (cortisol mean values given in brackets): benign breast tumors (95), gall stones (107), ulcer of the stomach and duodenum (96), hernia (78), appendicitis acuta (112), and struma (90). The results are in accordance with the hypothesis that glucocorticoids are involved in the increased protein catabolism of skeletal muscles and other signs of cachectic tumor patients.
|
['Adolescent', 'Adult', 'Aged', 'Breast Neoplasms', 'Cachexia', 'Female', 'Gastrointestinal Neoplasms', 'Humans', 'Hydrocortisone', 'Ketoglutaric Acids', 'Male', 'Middle Aged', 'Neoplasms', 'Radioligand Assay']
| 468,889
|
[]
|
[]
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Vascular damage mediates neuronal and non-neuronal pathology following short and long-term rotenone administration in Sprague-Dawley rats.
|
Even though rotenone has been used extensively in recent years to produce a model of Parkinson disease in rats, its systemic effects either on neurons apart from dopaminergic structures or non-neuronal tissues have not been elucidated well. In our present study, 30 adult Sprague-Dawley rats were divided into three even groups. A short-term rotenone-treated group received 10 mg/kg b.w. rotenone daily for 7 days. The long-term rotenone-treated group received 3 mg/kg b.w. rotenone daily for 30 days. The control group received vehicle only and were kept 5 rats each in parallel to both short- and long-term rotenone treated groups. It was found that short-term rotenone treatment produced marked vascular damages associated with ischemic neuronal degeneration particularly in the thalamus, cerebellum and nucleus dentatus. In long-term rotenone-treated group, vascular changes were less severe and neuronal degeneration was associated with mild microglial proliferation and astrocytosis. Non-neuronal pathology as the result of short-term rotenone exposure consisted of degeneration and necrosis of seminiferous tubular epithelia with formation of spermatide multinucleate giant cells. On the other hand, long-term rotenone treatment did not affect testicles and only caused sinusoidal dilatation in the liver, myocardial degeneration in the heart and interstitial hemorrhages in the kidneys and lungs. In conclusions, damage to blood vasculature by rotenone appeared mediating neuronal and non-neuronal pathology in Sprague-Dawley rats. This effect might provide new insights for ethiopathogenesis of neurodegenerative diseases and contributes to the understanding of hemorrhagic stroke.
|
['Animals', 'Brain', 'Dopamine', 'Dose-Response Relationship, Drug', 'Insecticides', 'Ischemia', 'Male', 'Neurons', 'Organ Specificity', 'Rats', 'Rats, Sprague-Dawley', 'Rotenone', 'Time Factors', 'Vascular Diseases']
| 21,676,605
|
[['B01.050'], ['A08.186.211'], ['D02.092.211.215.406', 'D02.092.311.342', 'D02.455.426.559.389.657.166.175.342'], ['G07.690.773.875', 'G07.690.936.500'], ['D27.720.031.700.491', 'D27.888.723.491'], ['C23.550.513'], ['A08.675', 'A11.671'], ['G07.650'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.750'], ['D03.383.663.283.266.450.400.843', 'D03.633.100.150.266.450.400.843', 'D03.633.400.825'], ['G01.910.857'], ['C14.907']]
|
['Organisms [B]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[Auditory evoked potentials and magnetic resonance in the diagnosis and follow-up of central pontine myelinolysis].
|
We report the case of a woman who developed central pontine myelinolysis in spite of gradual correction of hyponatremia. The good clinical evolution as well as the influence of rapid correction of serum sodium concentration in the unfolding of this rare condition are discussed. Several diagnostic procedures, mainly auditory-evoked responses and magnetic resonance imaging are also analysed. We remark the particular interest of the auditory-evoked responses in the attestation of an eventual remyelination.
|
['Aged', 'Demyelinating Diseases', 'Evoked Potentials, Auditory', 'Female', 'Humans', 'Magnetic Resonance Imaging', 'Pons']
| 8,480,063
|
[['M01.060.116.100'], ['C10.314'], ['G07.265.216.500.370', 'G07.888.250', 'G11.561.200.500.370'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.350.825.500'], ['A08.186.211.132.810.428.600']]
|
['Named Groups [M]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
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