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Unbalance of L-lysine flux in Corynebacterium glutamicum and its use for the isolation of excretion-defective mutants.
|
We found that the simple addition of L-methionine to the wild type of Corynebacterium glutamicum results in excretion of the cellular building block L-lysine up to rates of 2.5 nmol/min/mg (dry weight). Biochemical analyses revealed that L-methionine represses the homoserine dehydrogenase activity and reduces the intracellular L-threonine level from 7 to less than 2 mM. Since L-lysine synthesis is regulated mainly by L-threonine (plus L-lysine) availability, the result is enhanced flux towards L-lysine. This indicates a delicate and not well controlled type of flux control at the branch point of aspartate semialdehyde conversion to either L-lysine or L-threonine, probably due to the absence of isoenzymes in C. glutamicum. The inducible system of L-lysine excretion discovered was used to isolate mutants defective in the excretion of this amino acid. One such mutant characterized in detail accumulated 174 mM L-lysine in its cytosol without extracellular excretion of L-lysine, whereas the wild type accumulated 53 mM L-lysine in the cytosol and 5.9 mM L-lysine in the medium. The mutant was unaffected in L-lysine uptake or L-isoleucine or L-glutamate excretion, and also the membrane potential was unaltered. This mutant therefore represents a strain with a defect in an excretion system for the primary metabolite L-lysine.
|
['Biological Transport', 'Corynebacterium', 'Glutamic Acid', 'Homoserine Dehydrogenase', 'Isoleucine', 'Lysine', 'Methionine', 'Mutation']
| 7,608,075
|
[['G03.143'], ['B03.510.024.250', 'B03.510.460.400.400.200'], ['D12.125.067.625.349', 'D12.125.119.409.349', 'D12.125.427.300'], ['D08.811.682.047.820.300'], ['D12.125.070.577', 'D12.125.142.383'], ['D12.125.068.555', 'D12.125.095.647', 'D12.125.142.497'], ['D02.886.030.676', 'D12.125.142.557', 'D12.125.154.549', 'D12.125.166.676'], ['G05.365.590']]
|
['Phenomena and Processes [G]', 'Organisms [B]', 'Chemicals and Drugs [D]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
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Comparative high-density microarray analysis of gene expression during growth of Lactobacillus helveticus in milk versus rich culture medium.
|
Lactobacillus helveticus CNRZ32 is used by the dairy industry to modulate cheese flavor. The compilation of a draft genome sequence for this strain allowed us to identify and completely sequence 168 genes potentially important for the growth of this organism in milk or for cheese flavor development. The primary aim of this study was to investigate the expression of these genes during growth in milk and MRS medium by using microarrays. Oligonucleotide probes against each of the completely sequenced genes were compiled on maskless photolithography-based DNA microarrays. Additionally, the entire draft genome sequence was used to produce tiled microarrays in which noninterrupted sequence contigs were covered by consecutive 24-mer probes and associated mismatch probe sets. Total RNA isolated from cells grown in skim milk or in MRS to mid-log phase was used as a template to synthesize cDNA, followed by Cy3 labeling and hybridization. An analysis of data from annotated gene probes identified 42 genes that were upregulated during the growth of CNRZ32 in milk (P < 0.05), and 25 of these genes showed upregulation after applying Bonferroni's adjustment. The tiled microarrays identified numerous additional genes that were upregulated in milk versus MRS. Collectively, array data showed the growth of CNRZ32 in milk-induced genes encoding cell-envelope proteinases, oligopeptide transporters, and endopeptidases as well as enzymes for lactose and cysteine pathways, de novo synthesis, and/or salvage pathways for purines and pyrimidines and other functions. Genes for a hypothetical phosphoserine utilization pathway were also differentially expressed. Preliminary experiments indicate that cheese-derived, phosphoserine-containing peptides increase growth rates of CNRZ32 in a chemically defined medium. These results suggest that phosphoserine is used as an energy source during the growth of L. helveticus CNRZ32.
|
['Adaptation, Physiological', 'Animals', 'Carbocyanines', 'Culture Media', 'DNA, Bacterial', 'DNA, Complementary', 'Enzymes', 'Gene Expression Profiling', 'Gene Expression Regulation, Bacterial', 'Lactobacillus helveticus', 'Metabolic Networks and Pathways', 'Milk', 'Molecular Sequence Data', 'Nucleic Acid Hybridization', 'Oligonucleotide Array Sequence Analysis', 'RNA, Bacterial', 'Staining and Labeling']
| 17,322,329
|
[['G07.025', 'G16.012.500'], ['B01.050'], ['D02.455.326.397.300'], ['D27.720.470.305', 'E07.206'], ['D13.444.308.212'], ['D13.444.308.497.220', 'D13.444.600.223.500', 'D27.720.470.530.600.223.260'], ['D08.811'], ['E05.393.332'], ['G05.308.300'], ['B03.353.750.450.475.400', 'B03.510.460.400.410.475.475.400', 'B03.510.550.450.475.400'], ['G03.493'], ['A12.200.455', 'A12.790', 'G07.203.100.700', 'G07.203.300.350.525', 'J02.200.700', 'J02.500.350.525'], ['L01.453.245.667'], ['E05.393.661', 'G02.111.611'], ['E05.393.661.640', 'E05.393.760.640', 'E05.588.570.660', 'E05.601.640'], ['D13.444.735.473'], ['E01.370.225.500.620.670', 'E01.370.225.750.600.670', 'E05.200.500.620.670', 'E05.200.750.600.670']]
|
['Phenomena and Processes [G]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Technology, Industry, and Agriculture [J]', 'Information Science [L]']
| 1
| 1
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Development of fetal rat pancreatic islet A cells. A quantitative and immunocytochemical study.
|
OBJECTIVE: To perform a detailed quantitative immunocytochemical study of the development of fetal rat pancreatic islet A cells.METHODS: Pancreases were obtained from 19 and 21-day- old fetal rats. Ten rats were used per each group. Non-fasting blood glucose levels were measured to confirm that the animals were normoglycemic. The pregnant rats were anesthetized by ether inhalation, and the fetuses were removed from their uteruses. They were fixed in buffered neutral formalin, dehydrated and embedded in paraplast and serially sectioned (5 microm). We examined 32-48 islets (8-12 per section) for each fetus. Sections were stained by avidin biotin complex technique. A quantitative study was performed on the pancreatic islet A cells. Carl Zeiss software from Zeiss was used in this study. This study was carried out at the Department of Anatomy, King Abdul-Aziz University, Jeddah, Kingdom of Saudi Arabia during the period January to December 2005.RESULTS: The volume density and the number of A cells showed a significant increase during the last days of gestation. All other parameters showed a significant increase during the last days of gestation. The A cell nuclear diameter and volume did not increase significantly during the last days of pregnancy. The A cells were well stained and occupied the peripheral part of the islets.CONCLUSION: The present study represented a detailed quantitative immunohistochemical study and demonstrated that the size of the endocrine tissue and the islet A cells increased significantly during the last days of gestation.
|
['Animals', 'Cell Count', 'Cell Size', 'Female', 'Gestational Age', 'Glucagon-Secreting Cells', 'Immunohistochemistry', 'Islets of Langerhans', 'Rats', 'Saudi Arabia']
| 16,951,764
|
[['B01.050'], ['E01.370.225.500.195', 'E05.200.500.195', 'E05.242.195', 'G04.140'], ['G04.325'], ['G07.345.500.325.235.968', 'G08.686.320'], ['A03.734.414.065', 'A06.300.414.043', 'A06.390.087', 'A11.382.625.064', 'A11.436.294.064'], ['E01.370.225.500.607.512', 'E01.370.225.750.551.512', 'E05.200.500.607.512', 'E05.200.750.551.512', 'E05.478.583', 'H01.158.100.656.234.512', 'H01.158.201.344.512', 'H01.158.201.486.512', 'H01.181.122.573.512', 'H01.181.122.605.512'], ['A03.734.414', 'A06.300.414'], ['B01.050.150.900.649.313.992.635.505.700'], ['Z01.252.245.500.750']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Disciplines and Occupations [H]', 'Geographicals [Z]']
| 1
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Use of the bougie in simulated difficult intubation. 2. Comparison of single-use bougie with multiple-use bougie.
|
We studied the success rates for tracheal intubation in 32 healthy, anaesthetised patients during simulated grade IIIa laryngoscopy, randomised to either the multiple-use or the single-use bougie. Success rates (primary end-point) and times taken (secondary end-point) to achieve tracheal intubation were recorded. The multiple-use bougie was more successful than the single-use one (15/16 successful intubations vs. 9/16; p = 0.03). With either device, median [range] total tracheal intubation times for successful attempts were < 54 [24-84] s and there were no clinically important differences between these times. We conclude that the multiple-use bougie is a more reliable aid to tracheal intubation than the single-use introducer in grade IIIa laryngoscopy.
|
['Adolescent', 'Adult', 'Aged', 'Anesthesia, General', 'Disposable Equipment', 'Equipment Reuse', 'Female', 'Humans', 'Intubation, Intratracheal', 'Laryngoscopy', 'Male', 'Middle Aged', 'Time Factors']
| 12,911,356
|
[['M01.060.057'], ['M01.060.116'], ['M01.060.116.100'], ['E03.155.197'], ['E07.252'], ['E05.328', 'N06.850.585'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.041.500', 'E02.585.578', 'E05.497.578'], ['E01.370.386.460', 'E01.370.388.250.525', 'E04.502.250.525', 'E04.580.373'], ['M01.060.116.630'], ['G01.910.857']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Phenomena and Processes [G]']
| 0
| 1
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|
High power ultra-widely tuneable femtosecond pulses from a non-collinear optical parametric oscillator (NOPO).
|
We present an ultra-widely tunable non-collinear optical parametric oscillator with an average output power of more than 3 W and a repetition frequency of 34 MHz. The system is pumped by the second harmonic of a femtosecond Yb:KLu(WO4)2 thin-disk laser oscillator. The wavelength of the signal pulse can be rapidly tuned over a wide range from the visible to the NIR just by scanning the resonator length.
|
['Equipment Design', 'Infrared Rays', 'Lasers, Dye', 'Light', 'Optics and Photonics', 'Oscillometry']
| 22,274,438
|
[['E05.320'], ['G01.358.500.505.650.552', 'G01.590.540.552', 'G01.750.250.650.552', 'G01.750.770.578.552', 'G16.500.275.063.725.525.400', 'G16.500.750.775.525.400', 'N06.230.300.100.725.525.400'], ['E07.632.490.230', 'E07.710.520.230'], ['G01.358.500.505.650', 'G01.590.540', 'G01.750.250.650', 'G01.750.770.578'], ['H01.671.617', 'J01.293.688'], ['E05.654']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Disciplines and Occupations [H]', 'Technology, Industry, and Agriculture [J]']
| 0
| 0
| 0
| 0
| 1
| 0
| 1
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| 1
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| 1
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Coexistence of three specialist aphids on common milkweed, Asclepias syriaca.
|
Coexistence of host-specific herbivores on plants is believed to be governed by interspecific interactions, but few empirical studies have systematically unraveled these dynamics. We investigated the role of several factors in promoting coexistence among the aphids Aphis nerii, Aphis asclepiadis, and Myzocallis asclepiadis that all specialize on common milkweed (Asclepias syriaca). Competitive exclusion is thought to occur when interspecific competition is stronger than intraspecific competition. Consequently, we investigated whether predators, mutualists, or resource quality affected the strength of intra- vs. interspecific competition among aphids in factorial manipulations of competition with exposure to predation, ants, and variable plant genotypes in three separate experiments. In the predation x competition experiment, predators reduced aphid per capita growth by 66%, but the strength of intra- and interspecific competition did not depend on predators. In the ants x competition experiment, ants reduced per capita growth of A. nerii and M. asclepiadis (neither of which were mutualists with ants) by approximately one-half. In so doing, ants ameliorated the negative effects of these competitors on ant-tended A. asclepiadis by two-thirds, representing a novel benefit of ant-aphid mutualism. Nevertheless, ants alone did not explain the persistence of competitively inferior A. asclepiadis as, even in the presence of ants, interspecific competition remained stronger than intraspecific competition. In the plant genotype x competition experiment, both A. asclepiadis and M. asclepiadis were competitively inferior to A. nerii, with the strength of interspecific competition exceeding that of intraspecific competition by 83% and 23%, respectively. Yet these effects differed among milkweed genotypes, and there were one or more plant genotypes for each aphid species where coexistence was predicted. A synthesis of our results shows that predators play little or no role in preferentially suppressing competitively dominant A. nerii. Nonetheless, A. asclepiadis benefits from ants, and A. asclepiadis and M. asclepiadis may escape competitive exclusion by A. nerii on select milkweed genotypes. Taken as a whole, the coexistence of three host-specific aphid species sharing the same resource was promoted by the dual action of ants as antagonists and mutualists and by genetic diversity in the plant population itself.
|
['Animals', 'Ants', 'Aphids', 'Asclepias', 'Feeding Behavior', 'Host-Parasite Interactions', 'Predatory Behavior', 'Species Specificity', 'Spiders']
| 18,724,729
|
[['B01.050'], ['B01.050.500.131.617.720.500.500.875.205'], ['B01.050.500.131.617.412.165'], ['B01.650.940.800.575.912.250.456.500.153'], ['F01.145.113.547', 'F01.145.407', 'G07.203.650.353'], ['G16.527.200.400'], ['F01.145.113.111.600', 'F01.145.113.252.520'], ['G16.824'], ['B01.050.500.131.166.803']]
|
['Organisms [B]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
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|
Detection and differentiation of coccidian oocysts by real-time PCR and melting curve analysis.
|
Rapid and reliable detection and identification of coccidian oocysts are essential for animal health and foodborne disease outbreak investigations. Traditional microscopy and morphological techniques can identify large and unique oocysts, but they are often subjective and require parasitological expertise. The objective of this study was to develop a real-time quantitative PCR (qPCR) assay using melting curve analysis (MCA) to detect, differentiate, and identify DNA from coccidian species of animal health, zoonotic, and food safety concern. A universal coccidia primer cocktail was designed and employed to amplify DNA from Cryptosporidium parvum, Toxoplasma gondii, Cyclospora cayetanensis, and several species of Eimeria, Sarcocystis, and Isospora using qPCR with SYBR Green detection. MCA was performed following amplification, and melting temperatures (T(m)) were determined for each species based on multiple replicates. A standard curve was constructed from DNA of serial dilutions of T. gondii oocysts to estimate assay sensitivity. The qPCR assay consistently detected DNA from as few as 10 T. gondii oocysts. T(m) data analysis showed that C. cayetanensis, C. parvum, Cryptosporidium muris, T. gondii, Eimeria bovis, Eimeria acervulina, Isospora suis, and Sarcocystis cruzi could each be identified by unique melting curves and could be differentiated based on T(m). DNA of coccidian oocysts in fecal, food, or clinical diagnostic samples could be sensitively detected, reliably differentiated, and identified using qPCR with MCA. This assay may also be used to detect other life-cycle stages of coccidia in tissues, fluids, and other matrices. MCA studies on multiple isolates of each species will further validate the assay and support its application as a routine parasitology screening tool.
|
['Animals', 'Cats', 'Cattle', 'Coccidia', 'DNA Primers', 'DNA, Protozoan', 'Feces', 'Humans', 'Oocysts', 'Polymerase Chain Reaction', 'Sensitivity and Specificity', 'Swine', 'Transition Temperature']
| 21,506,835
|
[['B01.050'], ['B01.050.150.900.649.313.750.377.750.250.125'], ['B01.050.150.900.649.313.500.380.271'], ['B01.043.075.189'], ['D13.695.578.424.450.275', 'D27.720.470.530.600.223.600'], ['D13.444.308.442'], ['A12.459'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A11.870.740.600', 'B05.500.675', 'B05.775.740.600', 'G07.345.500.550.500.675'], ['E05.393.620.500'], ['E05.318.370.800', 'E05.318.740.872', 'G17.800', 'N05.715.360.325.700', 'N05.715.360.750.725', 'N06.850.520.445.800', 'N06.850.520.830.872'], ['B01.050.150.900.649.313.500.880'], ['G01.906.595.850']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Reliability of an Online Geriatric Assessment Procedure Using the interRAI Acute Care Assessment System.
|
OBJECTIVES: To determine whether geriatric triage decisions made using a comprehensive geriatric assessment (CGA) performed online are less reliable than face-to-face (FTF) decisions.DESIGN: Multisite noninferiority prospective cohort study. Two specialist geriatricians assessed individuals sequentially referred for an acute care geriatric consultation. Participants were allocated to one FTF assessment and an additional assessment (FTF or online (OL)), creating two groups-two FTF (FTF-FTF, n = 81) or online and FTF (OL-FTF, n = 85).SETTING: Three acute care public hospitals in two Australian states.PARTICIPANTS: Admitted individuals referred for CGA.INTERVENTION: Nurse-administered CGA, based on the interRAI Acute Care assessment system accessed online and other online clinical data such as pathology results and imaging enabling geriatricians to review participants' information and provide input into their care from a distance.MEASUREMENTS: The primary decision subjected to this analysis was referral for permanent residential care. Geriatricians also recorded recommendations for referrals and variations for medication management and judgment regarding prognosis at discharge and after 3 months.RESULTS: Overall percentage agreement was 88% (n = 71) for the FTF-FTF group and 91% (n = 77) for the OL-FTF group. The difference in agreement between the FTF-FTF and OL-FTF groups was -3%, indicating that there was no difference between the methods of assessment. Judgements made regarding diagnoses of geriatric syndromes, medication management, and prognosis (with regard to hospital outcome and location at 3 months) were found to be equally reliable in each mode of consultation.CONCLUSION: Geriatric assessment performed online using a nurse-administered structured CGA system was no less reliable than conventional assessment in making clinical triage decisions.
|
['Aged', 'Australia', 'Female', 'Geriatric Assessment', 'Hospitalization', 'Humans', 'Internet', 'Male', 'Prognosis', 'Prospective Studies', 'Referral and Consultation', 'Reproducibility of Results', 'Surveys and Questionnaires', 'Telemedicine', 'Triage']
| 28,832,897
|
[['M01.060.116.100'], ['Z01.639.100', 'Z01.678.100.373'], ['E05.318.308.225', 'I01.240.425.350', 'N01.224.425.350', 'N05.715.360.300.360', 'N06.850.505.400.425.350', 'N06.850.520.308.225'], ['E02.760.400', 'N02.421.585.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['L01.224.230.110.500'], ['E01.789'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['N04.452.758.849'], ['E05.318.370.725', 'E05.337.851', 'N05.715.360.325.685', 'N06.850.520.445.725'], ['E05.318.308.980', 'N05.715.360.300.800', 'N06.850.520.308.980'], ['H02.403.840', 'L01.178.847.652', 'N04.590.374.800'], ['N02.421.297.900']]
|
['Named Groups [M]', 'Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]', 'Organisms [B]', 'Information Science [L]', 'Disciplines and Occupations [H]']
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 1
| 1
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| 1
|
The founding of Mauritian endemic coffee trees by a synchronous long-distance dispersal event.
|
The stochastic process of long-distance dispersal is the exclusive means by which plants colonize oceanic islands. Baker's rule posits that self-incompatible plant lineages are unlikely to successfully colonize oceanic islands because they must achieve a coordinated long-distance dispersal of sufficiently numerous individuals to establish an outcrossing founder population. Here, we show for the first time that Mauritian Coffea species are self-incompatible and thus represent an exception to Baker's rule. The genus Coffea (Rubiaceae) is composed of approximately 124 species with a paleotropical distribution. Phylogenetic evidence strongly supports a single colonization of the oceanic island of Mauritius from either Madagascar or Africa. We employ Bayesian divergence time analyses to show that the colonization of Mauritius was not a recent event. We genotype S-RNase alleles from Mauritian endemic Coffea, and using S-allele gene genealogies, we show that the Mauritian allelic diversity is confined to just seven deeply divergent Coffea S-RNase allelic lineages. Based on these data, we developed an individual-based model and performed a simulation study to estimate the most likely number of founding individuals involved in the colonization of Mauritius. Our simulations show that to explain the observed S-RNase allelic diversity, the founding population was likely composed of fewer than 31 seeds that were likely synchronously dispersed from an ancestral mainland species.
|
['Bayes Theorem', 'Coffea', 'Genes, Plant', 'Genotype', 'Mauritius', 'Models, Genetic', 'Phylogeny', 'Population Dynamics', 'Seed Dispersal', 'Sequence Analysis, DNA']
| 24,797,428
|
[['E05.318.740.600.200', 'N05.715.360.750.625.150', 'N06.850.520.830.600.200'], ['B01.650.940.800.575.912.250.456.937.275'], ['G05.360.340.024.340.393', 'G05.360.340.365.500'], ['G05.380'], ['Z01.639.520.520'], ['E05.599.395.397'], ['G05.697', 'G16.075.605', 'L01.100.697'], ['I01.240.600', 'N01.224.625', 'N06.850.505.400.700'], ['G01.154.767', 'G15.620.500', 'G15.808'], ['E05.393.760.700']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Geographicals [Z]', 'Information Science [L]', 'Anthropology, Education, Sociology, and Social Phenomena [I]']
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 1
| 0
| 1
| 0
| 1
| 1
|
The Weekend Effect in AAA Repair.
|
BACKGROUND: Conflicting reports exist regarding whether patients undergoing surgery on the weekend or later in the week experience worse outcomes.METHODS: We identified patients undergoing abdominal aortic aneurysm (AAA) repair in the Vascular Quality Initiative between 2009 and 2017 [n = 38,498; 30,537 endovascular aneurysm repair (EVAR) and 7961 open repair]. We utilized mixed effects logistic regression to compare adjusted rates of perioperative mortality based on the day of repair.RESULTS: Tuesday was the most common day for elective repair (22%), Friday for symptomatic repairs (20%), and ruptured aneurysms were evenly distributed. Patients with ruptured aneurysms experienced similar adjusted mortality whether they underwent repair during the week or on weekends. Transfers of ruptured AAA were more common over the weekend. However, patients transferred on the weekend experienced higher adjusted mortality than those transferred during the week (28% vs 21%, P = 0.02), despite the fact that during the week, transferred patients actually experienced lower adjusted mortality than patients treated at the index hospital (21% vs 31%, P < 0.01). Among symptomatic patients, adjusted mortality was higher for those undergoing repair over the weekend than those whose surgeries were delayed until a weekday (7.9% vs 3.1%, P = 0.02). Adjusted mortality in elective cases did not vary across the days of the week. Results were consistent between open and EVAR patients.CONCLUSION: We found no evidence of a weekend effect for ruptured or symptomatic AAA repair. However, patients with ruptured AAA transferred on the weekend experienced higher mortality than those transferred during the week, suggesting a need for improvement in weekend transfer processes.
|
['Aged', 'Aged, 80 and over', 'Aortic Aneurysm, Abdominal', 'Aortic Rupture', 'Blood Vessel Prosthesis Implantation', 'Endovascular Procedures', 'Female', 'Humans', 'Logistic Models', 'Male', 'Middle Aged', 'Postoperative Complications', 'Retrospective Studies', 'Survival Rate', 'Time Factors', 'Treatment Outcome']
| 31,082,917
|
[['M01.060.116.100'], ['M01.060.116.100.080'], ['C14.907.055.239.075', 'C14.907.109.139.075'], ['C14.907.055.185.125', 'C14.907.055.239.175', 'C14.907.109.139.175', 'C26.761.125'], ['E04.100.814.868.500', 'E04.650.200'], ['E04.100.814.529', 'E04.502.382'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.740.500.525', 'E05.318.740.600.800.450', 'E05.318.740.750.450', 'E05.599.835.875', 'N05.715.360.750.530.480', 'N05.715.360.750.625.700.450', 'N05.715.360.750.695.470', 'N06.850.520.830.500.525', 'N06.850.520.830.600.800.450', 'N06.850.520.830.750.450'], ['M01.060.116.630'], ['C23.550.767'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E05.318.308.985.550.900', 'N01.224.935.698.826', 'N06.850.505.400.975.550.900', 'N06.850.520.308.985.550.900'], ['G01.910.857'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Named Groups [M]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Health Care [N]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Effects of transforming growth factor-beta1 on renal extracellular matrix components and their regulating proteins.
|
Transforming growth factor-beta1 (TGF-beta1) is widely regarded as a potent fibrogenic renal growth factor. In cell culture, TGF-beta1 has been shown to increase various extracellular matrix (ECM) proteins and tissue inhibitors of metalloproteinases (TIMP), while decreasing matrix metalloproteinases (MMP), providing the optimum environment for progressive ECM accumulation. This study, which uses the isolated perfused rat kidney (IPRK), describes for the first time in a whole kidney preparation the action of TGF-beta1 on factors associated with ECM processing. This model allows the study of the intact rat kidney with physiologic cell-cell interactions in the absence of confounding systemic influences. Left kidneys were removed from male Wistar rats by a nonischemic technique and perfused with a sterile, apyrogenic, endotoxin-free perfusate, based on the plasma volume expander Hemaccel (polygeline), at constant pressure in a recirculating IPRK system. Kidneys were perfused for 1 h either with (n = 3) or without (n = 3) recombinant human TGF-beta1 (20 ng/ml). The effects of perfusion were controlled by comparison with the nonperfused contralateral kidney (n = 6). TGF-beta1 was measured in the perfusate and urine, at the start and end of the experiment using an enzyme-linked immunosorbent assay to its biologically active form. After perfusion, sections of the kidneys were analyzed for changes in mRNA by Northern blotting. Significant increases in mRNA for fibronectin (7.5-fold, P < 0.01), heparan sulfate proteoglycan core protein (53-fold, P < 0.001), laminin beta1 (12-fold, P < 0.001), collagen alpha1(IV) (17-fold, P < 0.001), collagen alpha1(III) (fourfold, P < 0.001), and MMP9 (twofold, P < 0.05) were observed after perfusion with TGF-beta1. Measurement of TIMP1, TIMP2, TIMP3, MMP1, and MMP2 mRNA demonstrated no detectable change, whereas determination of mRNA for tissue transglutaminase, an enzyme capable of cross-linking many ECM components, showed an eightfold increase (P < 0.01). This study suggests that in the IPRK and in the absence of other exogenous growth factors, TGF-beta1 selectively increases the synthesis of ECM and tissue transglutaminase without changes that would result in the reduction of ECM degradation.
|
['Animals', 'Base Sequence', 'Blotting, Northern', 'Dose-Response Relationship, Drug', 'Enzyme-Linked Immunosorbent Assay', 'Extracellular Matrix Proteins', 'Humans', 'Kidney', 'Kidney Function Tests', 'Male', 'Membrane Proteins', 'Metalloendopeptidases', 'Molecular Sequence Data', 'Peptidylprolyl Isomerase', 'Polymerase Chain Reaction', 'RNA, Messenger', 'Rats', 'Rats, Wistar', 'Reference Values', 'Sensitivity and Specificity', 'Transforming Growth Factor beta']
| 10,505,687
|
[['B01.050'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['E05.196.401.095', 'E05.301.300.074', 'E05.601.100'], ['G07.690.773.875', 'G07.690.936.500'], ['E05.478.566.350.170', 'E05.478.566.380.360', 'E05.478.583.400.170', 'E05.601.470.350.170', 'E05.601.470.380.360'], ['D12.776.860.300'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A05.810.453'], ['E01.370.390.400'], ['D12.776.543'], ['D08.811.277.656.300.480', 'D08.811.277.656.675.374'], ['L01.453.245.667'], ['D08.811.399.325.500'], ['E05.393.620.500'], ['D13.444.735.544'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.900'], ['E05.978.810'], ['E05.318.370.800', 'E05.318.740.872', 'G17.800', 'N05.715.360.325.700', 'N05.715.360.750.725', 'N06.850.520.445.800', 'N06.850.520.830.872'], ['D12.644.276.374.687', 'D12.644.276.954.775', 'D12.776.467.374.687', 'D12.776.467.942.775', 'D23.529.374.687', 'D23.529.942.775']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Health Care [N]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 1
| 0
|
Functional connection between the Clb5 cyclin, the protein kinase C pathway and the Swi4 transcription factor in Saccharomyces cerevisiae.
|
The rsf12 mutation was isolated in a synthetic lethal screen for genes functionally interacting with Swi4. RSF12 is CLB5. The clb5 swi4 mutant cells arrest at G(2)/M due to the activation of the DNA-damage checkpoint. Defects in DNA integrity was confirmed by the increased rates of chromosome loss and mitotic recombination. Other results suggest the presence of additional defects related to morphogenesis. Interestingly, genes of the PKC pathway rescue the growth defect of clb5 swi4, and pkc1 and slt2 mutations are synthetic lethal with clb5, pointing to a connection between Clb5, the PKC pathway, and Swi4. Different observations suggest that like Clb5, the PKC pathway and Swi4 are involved in the control of DNA integrity: there is a synthetic interaction between pkc1 and slt2 with rad9; the pkc1, slt2, and swi4 mutants are hypersensitive to hydroxyurea; and the Slt2 kinase is activated by hydroxyurea. Reciprocally, we found that clb5 mutant is hypersensitive to SDS, CFW, latrunculin B, or zymolyase, which suggests that, like the PKC pathway and Swi4, Clb5 is related to cell integrity. In summary, we report numerous genetic interactions and phenotypic descriptions supporting a close functional relationship between the Clb5 cyclin, the PKC pathway, and the Swi4 transcription factor.
|
['Blotting, Western', 'Cell Cycle', 'Chromosomes, Fungal', 'Cyclin B', 'DNA, Fungal', 'DNA-Binding Proteins', 'Flow Cytometry', 'Fluorescent Antibody Technique, Indirect', 'Hydroxyurea', 'Immunoprecipitation', 'Mutation', 'Protein Kinase C', 'Recombination, Genetic', 'Saccharomyces cerevisiae', 'Saccharomyces cerevisiae Proteins', 'Transcription Factors']
| 16,118,191
|
[['E05.196.401.143', 'E05.301.300.096', 'E05.478.566.320.200', 'E05.601.262', 'E05.601.470.320.200'], ['G04.144'], ['A11.284.187.360', 'A19.311', 'G05.360.162.360'], ['D12.644.360.262.120', 'D12.776.167.218.120', 'D12.776.476.262.120'], ['D13.444.308.300'], ['D12.776.260'], ['E01.370.225.500.363.342', 'E01.370.225.500.386.350', 'E05.196.712.516.600.240.350', 'E05.200.500.363.342', 'E05.200.500.386.350', 'E05.242.363.342', 'E05.242.386.350'], ['E01.370.225.500.607.512.240.310', 'E01.370.225.750.551.512.240.310', 'E05.200.500.607.512.240.310', 'E05.200.750.551.512.240.310', 'E05.478.583.375.310'], ['D02.065.950.395'], ['E05.196.150.639', 'E05.478.605'], ['G05.365.590'], ['D08.811.913.696.620.682.700.725'], ['G05.728'], ['B01.300.107.795.785.800', 'B01.300.930.705.655'], ['D12.776.354.750'], ['D12.776.930']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Organisms [B]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[New class of RP4 plasmid mutations inducing a mucoid-type of Escherichia coli K-12 cell growth].
|
A new class of mutations is described which induce mucoid growth of Escherichia coli K-12. Unlike classical capR and capS mutations, the mucoid phenotype of colonies of the cap forms obtained is determined by mutations in genomes of thermosensitive plasmids pEG1 and RP1-6Repts12, derivates of the RP1 Inc P1 Ap Tc Km factor and accompanied by a complete or partial loss of the thermosensitive character of maintenance. The morphological character induced by plasmids is not associated with changes in the sensitivity of bacteria to UV irradiation and is determined by superproduction of capsular polysaccharide differing in the chemical structure from colanic acid, a common capsular polysaccharide of E. coli K-12.
|
['Conjugation, Genetic', 'Escherichia coli', 'Genes, Bacterial', 'Mutation', 'Phenotype', 'Plasmids', 'Polysaccharides, Bacterial', 'Transformation, Bacterial']
| 7,049,833
|
[['G05.728.200'], ['B03.440.450.425.325.300', 'B03.660.250.150.180.100'], ['G05.360.340.024.340.364.249', 'G05.360.340.358.024.249', 'G05.360.340.358.207.249'], ['G05.365.590'], ['G05.695'], ['G05.360.600'], ['D09.698.718', 'D23.050.161.616'], ['E05.393.350.810.500', 'G05.728.860.500', 'G05.728.865.820', 'G06.099.850']]
|
['Phenomena and Processes [G]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Instrumentation for bedside analysis of swallowing disorders.
|
Disordered swallowing, or dysphagia, is a common problem seen in patients undergoing treatment for cancer, stroke and neurodegenerative illnesses. This disease is associated with aspiration-induced chest infections. The methods currently used for diagnosis, however, are qualitative or based on expensive equipment. Swallowing accelerometry is a promising low-cost, quantitative and noninvasive tool for the evaluation of swallowing. This work describes the design and application of a bedside instrument able to evaluate swallowing mechanisms and to identify patients at risk of aspiration. Three-axis swallowing accelerometry was used to measure the neck vibrations associated with deglutition, providing analog signals to a virtual instrument developed in LabVIEW environment. In vivo tests in normal subjects as well as tests with disphagic patients showed that the system was able to easily and non-invasively detect changes in the swallowing acceleration pattern associated with increasing values of water volume (p < 0.02) and disphagia. We concluded that the developed system could be a useful tool for the objective bedside evaluation of patients at risk of aspiration.
|
['Acceleration', 'Deglutition Disorders', 'Diagnosis, Computer-Assisted', 'Equipment Design', 'Equipment Failure Analysis', 'Female', 'Humans', 'Male', 'Monitoring, Physiologic', 'Point-of-Care Systems', 'Reproducibility of Results', 'Sensitivity and Specificity', 'Transducers', 'Young Adult']
| 21,096,774
|
[['G01.482.107'], ['C06.405.117.119', 'C09.775.174'], ['E01.158', 'L01.313.500.750.100.158'], ['E05.320'], ['E05.325.192'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.520'], ['N04.452.442.452.452.680', 'N04.452.515.360.652', 'N04.590.874'], ['E05.318.370.725', 'E05.337.851', 'N05.715.360.325.685', 'N06.850.520.445.725'], ['E05.318.370.800', 'E05.318.740.872', 'G17.800', 'N05.715.360.325.700', 'N05.715.360.750.725', 'N06.850.520.445.800', 'N06.850.520.830.872'], ['E07.305.812'], ['M01.060.116.815']]
|
['Phenomena and Processes [G]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Information Science [L]', 'Organisms [B]', 'Health Care [N]', 'Named Groups [M]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 1
| 1
| 0
|
Further investigation of the pathogenicity, immunogenicity and stability of precocious Eimeria acervulina.
|
Attenuated lines of Eimeria acervulina were isolated between 62 and 72 h post-infection from the Houghton (H) strain. The inoculation of small numbers of oocysts of precocious (HP) lines gave substantial protection to Light Sussex chicks kept on litter against challenge with the virulent H strain. The precocious trait of the 72 h HP line was shown to be stable because the kinetics of oocyst production remained unaltered after 9 consecutive passages through birds in which only late developing oocysts were used for passage. The precocious 62 h HP line was subjected to further selection for 5 consecutive passages. The resultant (62 A HP) line was shown to be more attenuated than the 72 h HP line but remained capable of immunizing chicks against challenge with the H strain.
|
['Animals', 'Chickens', 'Eimeria', 'Immunity', 'Immunization']
| 6,877,863
|
[['B01.050'], ['B01.050.150.900.248.350.150', 'B01.050.150.900.248.690.192'], ['B01.043.075.189.250.250.250'], ['G12.450'], ['E02.095.465.425.400', 'E05.478.550', 'N02.421.726.758.310', 'N06.850.780.200.425', 'N06.850.780.680.310']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Systematic review of visual rehabilitation interventions for oculomotor deficits in patients with brain injury.
|
Purpose: Secondary to brain injury, many people develop eye movement disorders (oculomotor deficits). To clarify, optimize, and standardize the development of oculomotor rehabilitation programs, we systematically reviewed the literature on vision rehabilitation interventions for oculomotor deficits in brain injury, focusing on those with broad clinical feasibility.Materials and Methods: We searched MEDLINE (PubMed), CENTRAL, Scopus, and CINAHL databases for key title terms "oculomotor", "rehabilitation", or a related term, and "brain injury" or a related term in the title or abstract. We excluded case reports of a single patient, studies of non-oculomotor visual deficits, and articles in which the intervention and assessment methods were not explicitly identified.Results: Nine articles were included, six of which utilized computer-based training programs to elicit characteristic fixation, saccades, pursuit, vergence, and accommodative movements. Within the entire sample, interventions ranged from 3 to 10 weeks, and involved 2 to 5 training sessions per week.Conclusions: Oculomotor rehabilitation interventions showed some efficacy in treating patients with brain injury; however, there were very few studies overall. Several eye movement types - fixation, saccades, pursuit, vergence, and accommodation - can be elicited manually by therapists. We eagerly await the development and implementation of new intervention programs for broad-based clinical practice.
|
['Brain Injuries', 'Clinical Trials as Topic', 'Eye Movements', 'Humans', 'Ocular Motility Disorders']
| 31,455,098
|
[['C10.228.140.199', 'C10.900.300.087', 'C26.915.300.200'], ['E05.318.372.250.250', 'N05.715.360.330.250.250', 'N06.850.520.450.250.250'], ['G11.427.410.140', 'G14.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C10.228.758', 'C10.292.562', 'C11.590']]
|
['Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
MAESTRO--multi agent stability prediction upon point mutations.
|
BACKGROUND: Point mutations can have a strong impact on protein stability. A change in stability may subsequently lead to dysfunction and finally cause diseases. Moreover, protein engineering approaches aim to deliberately modify protein properties, where stability is a major constraint. In order to support basic research and protein design tasks, several computational tools for predicting the change in stability upon mutations have been developed. Comparative studies have shown the usefulness but also limitations of such programs.RESULTS: We aim to contribute a novel method for predicting changes in stability upon point mutation in proteins called MAESTRO. MAESTRO is structure based and distinguishes itself from similar approaches in the following points: (i) MAESTRO implements a multi-agent machine learning system. (ii) It also provides predicted free energy change (Ä ÄG) values and a corresponding prediction confidence estimation. (iii) It provides high throughput scanning for multi-point mutations where sites and types of mutation can be comprehensively controlled. (iv) Finally, the software provides a specific mode for the prediction of stabilizing disulfide bonds. The predictive power of MAESTRO for single point mutations and stabilizing disulfide bonds is comparable to similar methods.CONCLUSIONS: MAESTRO is a versatile tool in the field of stability change prediction upon point mutations. Executables for the Linux and Windows operating systems are freely available to non-commercial users from http://biwww.che.sbg.ac.at/MAESTRO.
|
['Disulfides', 'Internet', 'Point Mutation', 'Protein Stability', 'Proteins', 'User-Computer Interface']
| 25,885,774
|
[['D01.248.497.158.874.390', 'D01.875.350.850.150', 'D02.886.520.150'], ['L01.224.230.110.500'], ['G05.365.590.675'], ['G02.111.700'], ['D12.776'], ['L01.224.900.910']]
|
['Chemicals and Drugs [D]', 'Information Science [L]', 'Phenomena and Processes [G]']
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
[Transitional tumor of the right kidney with vena cava thrombus. Report of a case].
|
Transitional cell carcinoma of the kidney with vena caval tumor thrombus is a rarity with 12 cases reported in the literature. We review in this article the elements of the diagnosis and possible treatment modalities.
|
['Aged', 'Carcinoma, Transitional Cell', 'Humans', 'Kidney Neoplasms', 'Male', 'Neoplastic Cells, Circulating', 'Vena Cava, Inferior']
| 8,369,829
|
[['M01.060.116.100'], ['C04.557.470.200.430'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C04.588.945.947.535', 'C12.758.820.750', 'C12.777.419.473', 'C13.351.937.820.535', 'C13.351.968.419.473'], ['A11.642', 'C04.697.650.900', 'C23.550.727.650.900'], ['A07.015.908.949.648']]
|
['Named Groups [M]', 'Diseases [C]', 'Organisms [B]', 'Anatomy [A]']
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Three dimensional dental epithelial-mesenchymal constructs of predetermined size and shape for tooth regeneration.
|
While it is known that precise dental epithelial-mesenchymal (DE-DM) cell interactions provide critical functions in tooth development, reliable methods to establish proper DE-DM cell interactions for tooth regeneration have yet to be established. To address this challenge, and to generate bioengineered teeth of predetermined size and shape, in this study, we characterize three dimensional (3D) pre-fabricated DE-DM cell constructs. Human dental pulp cell seeded Collagen gel layers were co-cultured with porcine DE cells suspended in Growth Factor Reduced (GFR) Matrigel. The resulting 3D DE-DM cell layers were cultured in vitro, or implanted and grown subcutaneously in vivo in nude rats. Molecular, histological and immunohistochemical (IHC) analyses of harvested implants revealed organized DE-DM cell interactions, the induced expression of dental tissue-specific markers Amelogenin (AM) and Dentin Sialophosphoprotein (DSPP), and basement membrane markers Laminin 5 and collagen IV, and irregular mineralized tissue formation after 4 weeks. We anticipate that these studies will facilitate the eventual establishment of reliable methods to elaborate dental tissues, and full sized teeth of specified sized and shape.
|
['Adolescent', 'Animals', 'Cell Separation', 'Coculture Techniques', 'Epithelium', 'Fluorescence', 'Fluorescent Antibody Technique', 'Guided Tissue Regeneration', 'Humans', 'Male', 'Mesoderm', 'Microscopy, Confocal', 'Organ Size', 'Rats', 'Sus scrofa', 'Tissue Engineering', 'Tissue Scaffolds', 'Tooth']
| 20,682,455
|
[['M01.060.057'], ['B01.050'], ['E01.370.225.500.363', 'E05.200.500.363', 'E05.242.363'], ['E05.481.500.374'], ['A10.272'], ['G01.358.500.505.650.665.500', 'G01.590.540.665.500'], ['E01.370.225.500.607.512.240', 'E01.370.225.750.551.512.240', 'E05.200.500.607.512.240', 'E05.200.750.551.512.240', 'E05.478.583.375'], ['E04.680.300'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A16.504.660'], ['E01.370.350.515.395', 'E05.595.395'], ['E01.370.600.115.100.660', 'E05.041.124.715', 'G07.100.100.660', 'G07.345.249.690'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.500.880.399'], ['E05.481.500.311.500', 'J01.293.069.249.500'], ['E07.206.627', 'E07.695.825'], ['A14.549.167.860']]
|
['Named Groups [M]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Technology, Industry, and Agriculture [J]']
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
|
gp96-peptide vaccination of mice against intracellular bacteria.
|
This work demonstrates that gp96 preparations isolated from cells infected with intracellular bacteria induce cytotoxic T-lymphocyte responses and confer protection. Our findings extend previous reports on the immunogenicity of gp96-associated peptides to antigens derived from intracellular bacteria. Immunization with gp96 may therefore represent a promising vaccination strategy against bacterial pathogens.
|
['Animals', 'CD8-Positive T-Lymphocytes', 'Heat-Shock Proteins', 'Listeria monocytogenes', 'Listeriosis', 'Mice', 'Mice, Inbred C57BL', 'Mycobacterium tuberculosis', 'Peptides', 'Spleen', 'Tuberculosis', 'Vaccination']
| 11,349,093
|
[['B01.050'], ['A11.118.637.555.567.569.220', 'A15.145.229.637.555.567.569.220', 'A15.382.490.555.567.569.220'], ['D12.776.580.216'], ['B03.353.500.500.500', 'B03.510.100.500.500', 'B03.510.460.400.410.485.500'], ['C01.150.252.410.514'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.420', 'B01.050.150.900.649.313.992.635.505.500.400.420'], ['B03.510.024.962.500.702', 'B03.510.460.400.410.552.552.702'], ['D12.644'], ['A10.549.700', 'A15.382.520.604.700'], ['C01.150.252.410.040.552.846'], ['E02.095.465.425.400.530.890', 'E05.478.550.600.890', 'N02.421.726.758.310.890', 'N06.850.780.200.425.900', 'N06.850.780.680.310.890']]
|
['Organisms [B]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Neurovegetative disorders of perimenopausal women treated with docosahexaenoic acid (DHA, 625 mg).
|
The study evaluated the effect of DHA 625 mg in women who experience menopausal symptoms, on sexuality and quality of life (QoL), and on the auditory brainstem response (ABR). Forty-two perimenopausal women were enrolled. The Kupperman Index (KI) was used to evaluate menopause symptoms. The Short Form-36 (SF-36), Female Sexual Function Index (FSFI), and the Female Sexual Distress Scale (FSDS) were used to assess QoL, sexual function, and sexual distress, respectively. Auditory evoked potentials to measure the ABR. The study had one follow-up at 6 months. The women reported an improvement in the KI total score (p < .001). Moreover, women reported QoL improvements in all the psychological categories (p < .001), but not in physical categories (p = NS). FSFI and FSDS total scores increased (p < .01) and the FSDS score decreased (p < .01), mainly due to arousal (p < .03) and lubrication (p < .05) sexual aspects. The ABR wave latencies were lower than the baseline values (p < .05). DHA could be effective in modulating some perimenopausal symptoms in women and, consequently could contribute to improve their QoL and sexual life. Finally, DHA seems to have a direct activity on the neuronal conduction time into the audiological system.
|
['Docosahexaenoic Acids', 'Evoked Potentials, Auditory, Brain Stem', 'Female', 'Humans', 'Middle Aged', 'Perimenopause', 'Quality of Life', 'Sexual Dysfunction, Physiological']
| 28,562,107
|
[['D10.212.302.380.410.210', 'D10.251.355.337.250', 'D10.627.430.450.375'], ['G07.265.216.500.370.300', 'G07.888.250.300', 'G11.561.200.500.370.300'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['G08.686.157.500.562', 'G08.686.841.249.500.562'], ['I01.800', 'K01.752.400.750', 'N06.850.505.400.425.837'], ['C12.294.644', 'C13.351.500.665']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Named Groups [M]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Humanities [K]', 'Health Care [N]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 0
|
[Esophageal foreign bodies. Our ten years of experience].
|
OBJECTIVE: To study the management of esophageal foreign bodies.MATERIAL AND METHODS: A retrospective study was made of all rigid esophagoscopies performed for suspected foreign bodies in the esophagus by an otolaryngology department for ten years.RESULTS: Rigid esophagoscopy was performed for suspected foreign bodies in 46 patients (27 females, 19 males); age range 22 months to 88 years. In 40 cases an impacted foreign body was found. The most frequent location was the upper third of the esophagus (33/82.5%). The most common type of foreign body was chicken bones in adults (17/42) and coins in children (2/4). Nine patients (all adults) had complications.CONCLUSIONS: Due to its low cost and morbility, flexible endoscopy is the first choice for managing esophageal foreign bodies. Rigid esophagoscopy is still an appropriate technique when flexible endoscopy fails or it is not possible.
|
['Adolescent', 'Adult', 'Aged', 'Child', 'Esophagoscopy', 'Esophagus', 'Female', 'Foreign Bodies', 'Humans', 'Male', 'Middle Aged', 'Radiography', 'Retrospective Studies']
| 12,825,244
|
[['M01.060.057'], ['M01.060.116'], ['M01.060.116.100'], ['M01.060.406'], ['E01.370.372.250.250.275', 'E01.370.388.250.250.250.260', 'E04.210.240.250.260', 'E04.502.250.250.250.260'], ['A03.556.875.500'], ['C26.392'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['E01.370.350.700'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Diseases [C]', 'Organisms [B]', 'Health Care [N]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
A case of sudden cardiac death in connection with Salmonella typhimurium infection.
|
A case of fatal myocarditis in a 24-year-old otherwise healthy man is described. It was possible to cultivate Salmonella typhimurium from the alimentary tract, the blood, the liver and skeletal muscles. The possibility of a solitary myocarditis with fatal outcome due to Salmonella typhimurium infection is discussed. Such a case seems not to have been mentioned previously in the literature. The problems concerning the statistical registration of such a death are briefly discussed.
|
['Adult', 'Death, Sudden', 'Humans', 'Male', 'Myocarditis', 'Salmonella Infections', 'Salmonella typhimurium']
| 7,009,351
|
[['M01.060.116'], ['C23.550.260.322'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C14.280.238.625'], ['C01.150.252.400.310.821'], ['B03.440.450.425.800.200.825', 'B03.660.250.150.710.160.760']]
|
['Named Groups [M]', 'Diseases [C]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Effects of metoclopramide and metoclopramide/dopamine on blood pressure and insulin release in normotensive, hypertensive, and type 2 diabetic subjects.
|
The objective is to determine cardiovascular and insulin release effects under metoclopramide (MTC) and dopamine (DA) infusion by using an acute comparative design with the intravenous infusion of both drugs. We evaluated 15 normal (normotensive and normoglycemic) subjects, 13 hypertensive, and 15 type 2 diabetic subjects. Subjects were submitted to an experimental design in which we first gave them a 0.9% saline solution for 30 minutes, and then administered MTC at 7.5 microg kg min through an intravenous infusion during a period of 30 minutes. Although subjects were receiving MTC, we added an intravenous infusion of DA at 1-3 microg kg min during 30 minutes. Blood pressure, heart rate, serum lipid profile, and insulin levels were measured. Sympathetic reactivity by the cold pressor test was also measured. In normotensive subjects, there was a systolic blood pressure and heart rate increase during MTC plus DA infusion. In subjects with diabetes mellitus there was a heart rate increase without changes in blood pressure during the MTC plus DA infusion period. In hypertensive subjects, MTC induced a significant decrease of systolic and diastolic blood pressure. During MTC plus DA period there was an increase of heart rate but no significant changes in blood pressure. During cold pressor test in both diabetic and hypertensive subjects, there were significant increases of both blood pressure and heart rate. Insulin serum levels increased in normotensive and hypertensive subjects but were attenuated in subjects with diabetes mellitus. We conclude that there is a pharmacologic interaction between MTC and DA, that the pressor effects of DA are due to activation to beta and alpha adrenergic receptors, and that the cardiovascular effects of DA in type 2 diabetic subjects are attenuated by a probable defect in sympathetic system and to endothelial dysfunction.
|
['Adult', 'Blood Pressure', 'Cold Temperature', 'Diabetes Mellitus, Type 2', 'Dopamine', 'Dopamine Agents', 'Dopamine Antagonists', 'Drug Interactions', 'Endothelium, Vascular', 'Female', 'Heart Rate', 'Humans', 'Hypertension', 'Infusions, Intravenous', 'Insulin', 'Lipids', 'Male', 'Metoclopramide', 'Middle Aged', 'Receptors, Adrenergic, alpha', 'Receptors, Adrenergic, beta']
| 20,216,207
|
[['M01.060.116'], ['E01.370.600.875.249', 'G09.330.380.076'], ['G01.906.595.272', 'G16.500.275.063.725.710.300', 'G16.500.750.775.710.300', 'N06.230.300.100.725.154', 'N06.230.300.100.725.710.300'], ['C18.452.394.750.149', 'C19.246.300'], ['D02.092.211.215.406', 'D02.092.311.342', 'D02.455.426.559.389.657.166.175.342'], ['D27.505.519.625.150', 'D27.505.696.577.150'], ['D27.505.519.625.150.175', 'D27.505.696.577.150.175'], ['G07.690.773.968'], ['A07.015.700.500', 'A10.272.491.355'], ['E01.370.600.875.500', 'G09.330.380.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C14.907.489'], ['E02.319.267.082.500', 'E02.319.267.510.590'], ['D06.472.699.587.200.500.625', 'D12.644.548.586.200.500.625'], ['D10'], ['D02.065.277.573', 'D02.241.223.100.050.500.647', 'D02.241.223.100.100.510', 'D02.241.223.100.200.750', 'D02.241.223.100.300.350.625', 'D02.241.511.390.350.625', 'D02.455.426.559.389.127.020.937.647', 'D02.455.426.559.389.127.085.510', 'D02.455.426.559.389.127.250.750', 'D02.455.426.559.389.127.281.350.625', 'D02.455.426.559.389.657.654.638.625'], ['M01.060.116.630'], ['D12.776.543.750.670.300.300.300', 'D12.776.543.750.695.150.300.300', 'D12.776.543.750.720.330.300.300'], ['D12.776.543.750.670.300.300.340', 'D12.776.543.750.695.150.300.340', 'D12.776.543.750.720.330.300.340']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Organisms [B]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Dynamic evolution of mitochondrial ribosomal proteins in Holozoa.
|
We studied the highly dynamic evolution of mitochondrial ribosomal proteins (MRPs) in Holozoa. Most major clades within Holozoa are characterized by gains and/or losses of MRPs. The usefulness of gains of MRPs as rare genomic changes in phylogenetics is undermined by the high frequency of secondary losses. However, phylogenetic analyses of the MRP sequences provide evidence for the Acrosomata hypothesis, a sister group relationship between Ctenophora and Bilateria. An extensive restructuring of the mitochondrial genome and, as a consequence, of the mitochondrial ribosomes occurred in the ancestor of metazoans. The last MRP genes encoded in the mitochondrial genome were either moved to the nuclear genome or were lost. The strong decrease in size of the mitochondrial genome was probably caused by selection for rapid replication of mitochondrial DNA during oogenesis in the metazoan ancestor. A phylogenetic analysis of MRPL56 sequences provided evidence for a horizontal gene transfer of the corresponding MRP gene between metazoans and Dictyostelidae (Amoebozoa). The hypothesis that the requisition of additional MRPs compensated for a loss of rRNA segments in the mitochondrial ribosomes is corroborated by a significant negative correlation between the number of MRPs and length of the rRNA. Newly acquired MRPs evolved faster than bacterial MRPs and positions in eukaryote-specific MRPs were more strongly affected by coevolution than positions in prokaryotic MRPs in accordance with the necessity to fit these proteins into the pre-existing structure of the mitoribosome.
|
['Amoeba', 'Animals', 'Bacterial Proteins', 'Ctenophora', 'DNA, Mitochondrial', 'Evolution, Molecular', 'Gene Transfer, Horizontal', 'Genome, Mitochondrial', 'Genomics', 'Mitochondria', 'Mitochondrial Proteins', 'Phylogeny', 'RNA, Ribosomal', 'Ribosomal Proteins', 'Ribosomes', 'Sequence Analysis, DNA']
| 24,631,858
|
[['B01.046.500.100.700.089'], ['B01.050'], ['D12.776.097'], ['B01.050.500.325'], ['D13.444.308.283.225'], ['G05.045.250', 'G16.075.250'], ['G05.728.390'], ['G05.360.340.360'], ['H01.158.273.180.350', 'H01.158.273.343.350'], ['A11.284.430.214.190.875.564', 'A11.284.835.626'], ['D12.776.575'], ['G05.697', 'G16.075.605', 'L01.100.697'], ['D13.444.735.686'], ['D12.776.835'], ['A11.284.430.214.190.875.811'], ['E05.393.760.700']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Disciplines and Occupations [H]', 'Anatomy [A]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
|
Cardiovascular morbidity-mortality associated to ankle-brachial index in the general population.
|
BACKGROUND AND OBJECTIVES: Abnormal ankle-brachial index (ABI) is associated with a high risk of cardiovascular disease. This study has aimed to investigate the association between low ABI and risk of cardiovascular death in a general population attended in a primary care center.PATIENTS AND METHODS: A total of 1,361 volunteers aged between 60 and 79 years without any evidence of peripheral artery disease who attended a primary care center participated in the study. They underwent a complete physical examination, together with standard blood tests and ABI was determined. The participants were contacted by telephone 4 years later and asked about any cardiovascular problems for that period. Causes of death and hospitalization were confirmed in the medical records in the primary care center and/or hospital.RESULTS: Information was obtained about the clinical evolution of 1,300 participants (mean age 69.9 years, 38.2% men). Mean follow-up was 49.8 months. There were 13 cardiovascular death and 49 major cardiovascular events. Low ABI (<0.9) was associated with a significant higher risk of cardiovascular death (adjusted relative risk 6.83; 95% confidence interval 1.36-34.30, P=.020), and with a higher risk of major cardiovascular events (adjusted relative risk 2.42; 95% confidence interval 0.99-5.91, P=.051). High or uncompressible ABI was not associated with higher cardiovascular risk.CONCLUSIONS: A low ABI was associated with higher risk of cardiovascular death in the general population followed-up in a primary care center.
|
['Aged', 'Ankle Brachial Index', 'Cardiovascular Diseases', 'Female', 'Humans', 'Male', 'Middle Aged', 'Peripheral Arterial Disease', 'Risk Assessment', 'Risk Factors']
| 24,119,392
|
[['M01.060.116.100'], ['E01.370.370.140.049'], ['C14'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['C14.907.137.126.307.500', 'C14.907.617.671'], ['E05.318.740.600.800.715', 'N04.452.871.715', 'N05.715.360.750.625.700.690', 'N06.850.505.715', 'N06.850.520.830.600.800.715'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Organisms [B]', 'Health Care [N]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Quantitative multiplexed simulated-cell identification by SERS in microfluidic devices.
|
A reliable identification of cells on the basis of their surface markers is of great interest for diagnostic and therapeutic applications. We present a multiplexed labeling and detection strategy that is applied to four microparticle populations, each mimicking cellular or bacterial samples with varying surface concentrations of up to four epitopes, using four distinct biotags that are meant to be used in conjunction with surface enhanced Raman spectroscopy (SERS) instead of fluorescence, together with microfluidics. Four populations of 6 ìm polystyrene beads were incubated with different mixtures, "cocktails" of four SERS biotags (SBTs), simulating the approach that one would follow when seeking to identify multiple biomarkers encountered in biological applications. Populations were flowed in a microfluidic flow-focusing device and the SERS signal from individual beads was acquired during continuous flow. The spectrally rich SERS spectra enabled us to separate confidently the populations by utilizing principal component analysis (PCA). Also, using classical least squares (CLS), we were able to calculate the contributions of each SBT to the overall signal in each of the populations, and showed that the relative SBT contributions are consistent with the nominal percentage of each marker originally designed into that bead population, by functionalizing it with a given SBT cocktail. Our results demonstrate the multiplexing capability of SBTs in potential applications such as immunophenotyping.
|
['Biomarkers', 'Lab-On-A-Chip Devices', 'Spectrum Analysis, Raman']
| 26,404,699
|
[['D23.101'], ['E07.305.343.500'], ['E05.196.822.860', 'E05.196.867.890']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Improved Immune Responses in Young and Aged Mice with Adjuvanted Vaccines against H1N1 Influenza Infection.
|
Elderly people are at high risk for influenza-related morbidity and mortality due to progressive immunosenescence. While toll-like receptor (TLR) agonist containing adjuvants, and other adjuvants, have been shown to enhance influenza vaccine-induced protective responses, the mechanisms underlying how these adjuvanted vaccines could benefit the elderly remain elusive. Here, we show that a split H1N1 influenza vaccine (sH1N1) combined with a TLR4 agonist, glucopyranosyl lipid adjuvant formulated in a stable oil-in-water emulsion (GLA-SE), boosts IgG2c:IgG1 ratios, enhances hemagglutination inhibition (HAI) titers, and increases protection in aged mice. We find that all adjuvanted sH1N1 vaccines tested were able to protect both young and aged mice from lethal A/H1N1/California/4/2009 virus challenge after two immunizations compared to vaccine alone. We show that GLA-SE combined with sH1N1, however, also provides enhanced protection from morbidity in aged mice given one immunization (based on change in weight percentage). While the GLA-SE-adjuvanted sH1N1 vaccine promotes the generation of cytokine-producing T helper 1 cells, germinal center B cells, and long-lived bone marrow plasma cells in young mice, these responses were muted in aged mice. Differential in vitro responses, dependent on age, were also observed from mouse-derived bone marrow-derived dendritic cells and lung homogenates following stimulation with adjuvants, including GLA-SE. Besides enhanced HAI titers, additional protective factors elicited with sH1N1 + GLA-SE in young mice were observed, including (a) rapid reduction of viral titers in the lung, (b) prevention of excessive lung inflammation, and (c) homeostatic maintenance of alveolar macrophages (AMs) following H1N1 infection. Collectively, our results provide insight into mechanisms of adjuvant-mediated immune protection in the young and elderly.
|
['Adjuvants, Immunologic', 'Aged', 'Animals', 'Antibodies, Viral', 'Cells, Cultured', 'Dendritic Cells', 'Female', 'Glucosides', 'Humans', 'Immunity', 'Immunization', 'Influenza A Virus, H1N1 Subtype', 'Influenza Vaccines', 'Influenza, Human', 'Lipid A', 'Mice', 'Mice, Inbred C57BL', 'Orthomyxoviridae Infections', 'Toll-Like Receptor 4']
| 29,515,589
|
[['D27.505.696.477.067'], ['M01.060.116.100'], ['B01.050'], ['D12.776.124.486.485.114.254', 'D12.776.124.790.651.114.254', 'D12.776.377.715.548.114.254'], ['A11.251'], ['A11.066.270', 'A11.436.270', 'A15.382.066.270', 'A15.382.670.260'], ['D09.408.348'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G12.450'], ['E02.095.465.425.400', 'E05.478.550', 'N02.421.726.758.310', 'N06.850.780.200.425', 'N06.850.780.680.310'], ['B04.820.480.968.405.400.214'], ['D20.215.894.899.302'], ['C01.748.310', 'C01.925.782.620.365', 'C08.730.310'], ['D09.698.718.450.500', 'D10.494.500', 'D23.050.161.616.525.500', 'D23.946.123.329.500.500'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.420', 'B01.050.150.900.649.313.992.635.505.500.400.420'], ['C01.925.782.620'], ['D12.776.543.750.705.910.500.400']]
|
['Chemicals and Drugs [D]', 'Named Groups [M]', 'Organisms [B]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Plasma cholesteryl ester transfer protein mass and phospholipid transfer protein activity are associated with leptin in type 2 diabetes mellitus.
|
Adipose tissue contributes to plasma levels of lipid transfer proteins and is also the major source of plasma adipokines. We hypothesized that plasma cholesteryl ester transfer protein (CETP) mass, phospholipid transfer protein (PLTP) activity and cholesteryl ester transfer (CET, a measure of CETP action) are determined by adipokine levels. In this study, relationships of plasma CETP mass, PLTP activity and CET with leptin, resistin and adiponectin were analyzed in type 2 diabetic patients and control subjects. Plasma PLTP activity (P<0.001), CET (P<0.001), leptin (P=0.003), resistin (P<0.001), high sensitive C-reactive protein (P=0.005), and insulin resistance (HOMA(ir)) (P<0.001) were higher, whereas HDL cholesterol (P<0.001) and plasma adiponectin (P<0.001) were lower in 83 type 2 diabetic patients (32 females) than in 83 sex-matched control subjects. Multiple linear regression analysis demonstrated that in diabetic patients plasma leptin levels were related to plasma CETP mass (P=0.018) and PLTP activity (P<0.001), but not to the other adipokines measured. Plasma CET was inversely correlated with adiponectin in univariate analysis, but this association disappeared in multivariate models that included plasma lipids and CETP. In conclusion, both plasma CETP mass and PLTP activity are associated with plasma leptin in type 2 diabetes. The elevated CET in these patients is not independently related to any of the measured plasma adipokines.
|
['Blood Proteins', 'Cholesterol Ester Transfer Proteins', 'Diabetes Mellitus, Type 2', 'Female', 'Humans', 'Leptin', 'Lipids', 'Male', 'Middle Aged', 'Multivariate Analysis', 'Phospholipid Transfer Proteins']
| 17,185,032
|
[['D12.776.124'], ['D09.400.430.750', 'D12.776.124.197', 'D12.776.157.165', 'D12.776.395.199'], ['C18.452.394.750.149', 'C19.246.300'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D06.472.699.042.500', 'D12.644.276.024.500', 'D12.644.548.011.500', 'D12.776.467.024.500', 'D23.529.024.500'], ['D10'], ['M01.060.116.630'], ['E05.318.740.150.500', 'N05.715.360.750.125.500', 'N06.850.520.830.150.500'], ['D12.776.157.674', 'D12.776.543.693']]
|
['Chemicals and Drugs [D]', 'Diseases [C]', 'Organisms [B]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Pharmacological characterization of tachykinin-stimulated inositol phospholipid hydrolysis in peripheral tissues.
|
1. Tachykinin-stimulated inositol phospholipid hydrolysis was examined in slices of rat parotid gland, hamster urinary bladder and guinea-pig ileum longitudinal muscle. 2. In the presence of lithium, substance P and other naturally-occurring and synthetic tachykinins induced large, dose-dependent increases in [3H]-inositol monophosphate accumulation. 3. In slices of rat parotid gland, [pGlu6,L-Pro9]SP(6-11) was considerably more potent in stimulating inositol phospholipid hydrolysis than [pGlu6,D-Pro9]SP(6-11). 4. In contrast, in slices of hamster urinary bladder, [pGlu6,D-Pro9]SP(6-11) exhibited greater potency in evoking inositol phospholipid breakdown than [pGlu6,L-Pro9]SP(6-11). 5. The differential selectivity of these C-terminal fragments of substance P suggests that they may be useful tools for distinguishing between NK1 and NK2 receptors. 6. L-659,837 and L-659,874 antagonized eledoisin-stimulated inositol phospholipid hydrolysis in slices of hamster urinary bladder. Neither compound significantly reduced substance-P evoked inositol phospholipid breakdown in slices of rat parotid gland, or senktide-induced inositol phospholipid hydrolysis in slices of guinea-pig ileum. 7. L-659,837 and L-659,874 had no effect on the atropine-sensitive, carbachol-stimulated inositol phospholipid hydrolysis in slices of rat parotid gland. 8. These data further support the notion that L-659,837 and L-659,874 are potent and selective NK2 receptor antagonists.
|
['Animals', 'Cricetinae', 'Guinea Pigs', 'Hydrolysis', 'Ileum', 'In Vitro Techniques', 'Muscles', 'Parotid Gland', 'Peptide Fragments', 'Peptides, Cyclic', 'Phosphatidylinositols', 'Pyrrolidonecarboxylic Acid', 'Rats', 'Substance P', 'Tachykinins', 'Urinary Bladder']
| 1,707,702
|
[['B01.050'], ['B01.050.150.900.649.313.992.635.075.250'], ['B01.050.150.900.649.313.992.550'], ['G02.380'], ['A03.556.124.684.249', 'A03.556.249.124'], ['E05.481'], ['A02.633', 'A10.690'], ['A03.556.500.760.464', 'A10.336.779.464', 'A14.549.760.464'], ['D12.644.541'], ['D04.345.566', 'D12.644.641'], ['D10.570.755.375.760.400.942'], ['D03.383.773.812.718', 'D12.125.067.625.850', 'D12.125.072.401.761'], ['B01.050.150.900.649.313.992.635.505.700'], ['D12.644.276.812.900.866', 'D12.644.400.800.750', 'D12.644.456.800.866', 'D12.776.467.812.900.866', 'D12.776.631.650.800.750', 'D23.469.050.375.850.890', 'D23.529.812.900.866'], ['D12.644.276.812.900', 'D12.644.400.800', 'D12.644.456.800', 'D12.776.467.812.900', 'D12.776.631.650.800', 'D23.469.050.375.850', 'D23.529.812.900'], ['A05.810.890']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[Kidney failure after ectracorporeal circulation in cardiac surgery].
|
The renal function of 113 patients undergoing cardiac surgery under ECC was studied. In 32 p. 100 of the cases renal involvement was noted which was moderate in 18 p. 100 of the cases, severe in 10 p. 100 of the cases and anuric in 4 p. 100 of the cases. Valvular surgery was complicated once in every three cases by renal involvement, the repair of congenital malformations once out of every two cases, and coronary surgery in 17 p. 100 of the cases. The fall in renal perfusion represents the essential factor in this renal involvement, which should be avoided by the maintenace during ECC of a high output level and a satisfactory perfusion pressure and by the recovery of correct hemodynamics after the intervention.
|
['Acute Kidney Injury', 'Adolescent', 'Adult', 'Aged', 'Cardiac Surgical Procedures', 'Child', 'Child, Preschool', 'Coronary Artery Bypass', 'Extracorporeal Circulation', 'Heart Defects, Congenital', 'Heart Valve Prosthesis', 'Humans', 'Kidney', 'Middle Aged', 'Myocardial Infarction']
| 16,549
|
[['C12.777.419.780.050', 'C13.351.968.419.780.050'], ['M01.060.057'], ['M01.060.116'], ['M01.060.116.100'], ['E04.100.376', 'E04.928.220'], ['M01.060.406'], ['M01.060.406.448'], ['E04.100.376.719.332', 'E04.100.814.868.750', 'E04.928.220.520.220'], ['E04.292'], ['C14.240.400', 'C14.280.400', 'C16.131.240.400'], ['E07.695.310'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A05.810.453'], ['M01.060.116.630'], ['C14.280.647.500', 'C14.907.585.500', 'C23.550.513.355.750', 'C23.550.717.489.750']]
|
['Diseases [C]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Anatomy [A]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Characterization of a novel simian immunodeficiency virus from guereza colobus monkeys (Colobus guereza) in Cameroon: a new lineage in the nonhuman primate lentivirus family.
|
Exploration of the diversity among primate lentiviruses is necessary to elucidate the origins and evolution of immunodeficiency viruses. During a serological survey in Cameroon, we screened 25 wild-born guereza colobus monkeys (Colobus guereza) and identified 7 with HIV/SIV cross-reactive antibodies. In this study, we describe a novel lentivirus, named SIVcol, prevalent in guereza colobus monkeys. Genetic analysis revealed that SIVcol was very distinct from all other known SIV/HIV isolates, with average amino acid identities of 40% for Gag, 50% for Pol, 28% for Env, and around 25% for proteins encoded by five other genes. Phylogenetic analyses confirmed that SIVcol is genetically distinct from other previously characterized primate lentiviruses and clusters independently, forming a novel lineage, the sixth in the current classification. Cercopithecidae monkeys (Old World monkeys) are subdivided into two subfamilies, the Colobinae and the Cercopithecinae, and, so far, all Cercopithecidae monkeys from which lentiviruses have been isolated belong to the Cercopithecinae subfamily. Therefore, SIVcol from guereza colobus monkeys (C. guereza) is the first primate lentivirus identified in the Colobinae subfamily and the divergence of SIVcol may reflect divergence of the host lineage.
|
['Amino Acid Sequence', 'Animals', 'Antibodies, Viral', 'Base Sequence', 'Cameroon', 'Cloning, Molecular', 'Colobus', 'Female', 'HIV Antibodies', 'HIV Envelope Protein gp120', 'Lentivirus', 'Male', 'Membrane Glycoproteins', 'Molecular Sequence Data', 'Phylogeny', 'Polymerase Chain Reaction', 'Sequence Alignment', 'Sequence Analysis, DNA', 'Seroepidemiologic Studies', 'Simian Acquired Immunodeficiency Syndrome', 'Simian Immunodeficiency Virus', 'Viral Envelope Proteins']
| 11,134,299
|
[['G02.111.570.060', 'L01.453.245.667.060'], ['B01.050'], ['D12.776.124.486.485.114.254', 'D12.776.124.790.651.114.254', 'D12.776.377.715.548.114.254'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['Z01.058.290.100.110'], ['E05.393.220'], ['B01.050.150.900.649.313.988.400.112.199.150.150'], ['D12.776.124.486.485.114.254.150.440', 'D12.776.124.790.651.114.254.150.440', 'D12.776.377.715.548.114.254.150.440'], ['D12.776.964.775.325.164.249', 'D12.776.964.775.562.500.500', 'D12.776.964.970.880.325.164.249', 'D23.050.327.520.350'], ['B04.820.650.589'], ['D12.776.395.550', 'D12.776.543.550'], ['L01.453.245.667'], ['G05.697', 'G16.075.605', 'L01.100.697'], ['E05.393.620.500'], ['E05.393.751'], ['E05.393.760.700'], ['E05.318.372.500.950', 'N05.715.360.330.500.950', 'N06.850.520.450.500.950'], ['C01.925.782.815.616.850', 'C01.925.839.850', 'C22.735.500.850'], ['B04.820.650.589.650.800', 'B04.820.650.805.700'], ['D09.400.430.968', 'D12.776.395.550.993', 'D12.776.543.550.993', 'D12.776.964.970.880']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 1
| 1
|
Genotoxicity detected in wild mice living in a highly polluted wetland area in south western Spain.
|
A field study was carried out in the south of the Iberian Peninsula in an industrial area in the neighbourhood of Huelva city, SW Spain, and in a natural area (Do?ana National Park) for comparison, to estimate the genetic risk induced by environmental pollution in wild mice. Genotoxic effects in a sentinel organism, the Algerian mice (Mus spretus) free living in the industrial area were compared with animals of the same species living in the natural protected area. The single cell gel electrophoresis, or Comet assay, was performed as a genotoxicity test in peripheral blood of mice. Our results clearly show that mice free living in the contaminated area bear a high burden of genetic damage as compared with control individuals. The results suggest that the assessing of genotoxicity levels by the Comet assay in wild mice can be used as a valuable test in pollution monitoring and environmental conservation.
|
['Animals', 'Comet Assay', 'DNA Damage', 'Environmental Exposure', 'Environmental Monitoring', 'Environmental Pollution', 'Food Chain', 'Industrial Waste', 'Mice', 'Mining', 'Mutagens', 'Spain', 'Wetlands']
| 17,949,869
|
[['B01.050'], ['E05.196.401.153.150', 'E05.301.300.100.150', 'E05.393.560.150', 'E05.940.560.150'], ['G05.200'], ['N06.850.460.350'], ['N06.850.460.350.080', 'N06.850.780.375'], ['N06.850.460'], ['G16.500.275.157.250', 'N06.230.124.250'], ['D20.944.420', 'N06.850.460.710.420'], ['B01.050.150.900.649.313.992.635.505.500'], ['J01.576.655.875.500'], ['D27.888.569.468'], ['Z01.542.846'], ['G16.500.275.157.812', 'N06.230.124.625']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Chemicals and Drugs [D]', 'Technology, Industry, and Agriculture [J]', 'Geographicals [Z]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
|
[Isolation and identification of a newbacterial pathogen infecting larvae of honeybee (Apismellifera ) Perish].
|
A bacteroidal disease of honeybee (Apis mellifera ) larvae was found in some regions of Zhejiang Province, China , in early spring 2005. The diseased larvae lost its shine, became yellow and rotted when serious. This symptom was different to any bacteroidal disease of honeybee larval been reported. So, it is considered to be a new bacteroidal disease of honeybee larval. Five pure cultures of bacteria were separated from ten collections of diseased honeybee larvae, named as L1, L2, L3, IA and L5. Among these five pure cultures, only L2 could make the healthy honeybee larvae become diseased in both field and lab test. The symptom caused by L2 was similar to the natural-infection. From the diseased larvae caused by L2 could isolate bacteria the same as L2. Thus 12 was determined as the causing agent of this bacteroidal disease of honeybee larval. L2 was identified according to the characteristics of morphology, physiological biochemical characteristics and 16S rRNA gene sequence. As a result, the morphology and physiological biochemical characteristics of L2 were similar to E. faecium. And its 16S rRNA sequences highly matched to E. faecium, the similarities between them were higher than 99%. The overall similarity values between L2 and the published 16S rRNA sequences of 41 typical species of Enterococcus were 93.9% - 99.5% , the top value was between 12 and E. faecium. In the phylogenetic tree, L2 and E. faecium were assembled in the same ramification. So 12 was identified as E. faecium. Although Enterococcus faecium was known as pathogen to many post, account for 12% of all nosocomial infections, only second to E. coli, but there is no report about this bacteria infects honeybee up to now. So it is a new pathogen to honeybee. The isolation and identification of pathogen of this new bacteroidal larvae disease, afford a good feasibility for available prevention and cure to this new disease.
|
['Animals', 'Bees', 'Enterococcus faecium', 'Larva', 'Phylogeny', 'RNA, Ribosomal, 16S']
| 17,302,168
|
[['B01.050'], ['B01.050.500.131.617.720.500.500.875.387'], ['B03.353.750.250.250.300', 'B03.510.550.250.250.300'], ['B05.500.500', 'G07.345.500.550.500.500'], ['G05.697', 'G16.075.605', 'L01.100.697'], ['D13.444.735.686.670']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Chemicals and Drugs [D]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Association between interleukin 6 promoter variants and chronic hepatitis B progression.
|
Interleukin 6 (IL6) plays an essential role in the regulation of immune response to chronic disease. In this study, the three known single nucleotide polymorphisms (SNPs) in the IL6 promoter region were genotyped in a large chronic hepatitis B cohort to evaluate the effects of IL6 promoter variants. The single base extension method was used for this genotyping. Haplotypes were constructed by the three SNPs in IL6. Allele frequencies were compared for; i) patients with chronic hepatitis (CH) and chronic carriers vs. chronic hepatis patients with clinical evidence of liver cirrhosis (LC) (i.e., portal hypertension), ii) cirrhotic patients with hepatocellular carcinoma (HCC) vs. without HCC by logistic regression, and iii) with respect to the time intervals from the onset of infection to HCC. Results were analyzed by Cox relative hazard analysis on the assumption that all the patients were infected during early infancy. The frequencies of each SNP were 0.002 (IL6-597 G>A), 0.25 (IL6-572 C>G) and 0.002 (IL6-174 G>C), respectively, in the Korean population (n = 1,046). No significant associations were detected between IL6-572 C>G and chronic hepatitis B outcome in this study; i.e., LC occurrence on CH (OR = 0.16-1.27, P = 0.13- 0.71) and HCC occurrence on LC (OR = 1.04-1.23, P = 0.89-0.60) of heterozygotes and homozygotes for G allele in referent comparison to homozygotes for common allele (C/C genotype), and time interval to HCC (RH = 0.67-1.00; P = 0.14-0.99). In conclusion, there appeared to be no significant associations between IL6 promoter variants and disease outcome in chronic hepatitis B.
|
['Aged', 'Alleles', 'Asian Continental Ancestry Group', 'Female', 'Genetic Variation', 'Haplotypes', 'Hepatitis B, Chronic', 'Humans', 'Interleukin-6', 'Korea', 'Linkage Disequilibrium', 'Male', 'Middle Aged', 'Polymorphism, Single Nucleotide', 'Promoter Regions, Genetic']
| 12,754,410
|
[['M01.060.116.100'], ['G05.360.340.024.340.030'], ['M01.686.508.200'], ['G05.365'], ['G05.380.360'], ['C01.221.250.500.100', 'C01.925.256.430.400.100', 'C01.925.440.435.100', 'C06.552.380.350.100', 'C06.552.380.705.437.100'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.644.276.374.465.224', 'D12.776.467.374.465.202', 'D23.529.374.465.224'], ['Z01.252.474.557', 'Z01.586.407'], ['G05.348.500'], ['M01.060.116.630'], ['G05.365.795.598'], ['G02.111.570.080.689.675', 'G05.360.080.689.675', 'G05.360.340.024.340.137.750.680']]
|
['Named Groups [M]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Geographicals [Z]']
| 0
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 1
|
The protective effect of K+ channel openers on beta-amyloid induced cerebrovascular endothelial dysfunction.
|
Amyloid angiopathy is characterized by amyloid beta-peptide (A beta) deposition and may contribute to the cerebrovascular abnormalities that precede the onset of Alzheimer's Disease (AD). That aberrant potassium (K+) channel function occurs in AD patients is supported by deleterious effects of A beta on normal fibroblast K+ channels and prevention of A beta-induced toxicity by potassium channel openers (KCOs) in neuronal cell culture. We report here that KCOs protect cerebral and peripheral vessels against the endothelial damage induced by A beta. Pressurized posterior cerebral artery and aortic ring segments from the rat were constricted and then relaxed with the endothelium-dependent vasodilator acetylcholine before and after incubation with A beta (10(-6) M), or pre-treatment with KCOs before the addition of beta-amyloid. Vessels treated with A beta exhibited features of endothelial dysfunction: enhanced vasoconstriction and diminished endothelium-dependent vasodilation. Pre-treatment with KCOs significantly antagonized the A beta effect in both cerebral and aortic vessel segments. This protection was provided by both KCa and KATP channel openers. Endothelial damage by A beta and protection by KCOs was verified by electron microscopy. The K+ channel blocker, TEA, reversed the protective effect of KCO. The results suggest that potassium channel openers protect against A beta induced endothelial dysfunction and that KCOs may have a role in the treatment of degenerative cerebrovascular disease as seen in stroke, AD and aging.
|
['Acetylcholine', 'Aging', 'Alzheimer Disease', 'Amyloid beta-Peptides', 'Animals', 'Aorta', 'Aorta, Thoracic', 'Cells, Cultured', 'Cerebral Arteries', 'Cytoprotection', 'Endothelium, Vascular', 'Fibroblasts', 'Free Radicals', 'Male', 'Nitric Oxide', 'Potassium Channels', 'Rats', 'Rats, Sprague-Dawley']
| 10,406,005
|
[['D02.092.211.111'], ['G07.345.124'], ['C10.228.140.380.100', 'C10.574.945.249', 'F03.615.400.100'], ['D12.644.024', 'D12.776.049.407.249.500', 'D12.776.543.039.500'], ['B01.050'], ['A07.015.114.056'], ['A07.015.114.056.372'], ['A11.251'], ['A07.015.114.228'], ['G07.690.773.500'], ['A07.015.700.500', 'A10.272.491.355'], ['A11.329.228'], ['D01.339', 'D02.389'], ['D01.339.387', 'D01.625.550.500', 'D01.625.700.500', 'D01.650.550.587.600'], ['D12.776.157.530.400.600', 'D12.776.543.550.450.750', 'D12.776.543.585.400.750'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.750']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Organisms [B]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
A Quality Improvement Project to Improve Evidence-Based Inhaled Nitric Oxide Use.
|
BACKGROUND: Inhaled nitric oxide (INO) reduces extracorporeal membrane oxygenation (ECMO) use in term and near-term neonates with persistent pulmonary hypertension of the newborn; however, its overutilization is increasing. We hypothesized that implementing a shared baseline protocol would safely improve evidence-based INO use in a Level IV neonatal ICU.METHODS: Through several plan-do-study-act cycles, a shared baseline protocol for initiation and weaning of INO was developed and implemented starting in August 2014. Based on user feedback, the shared baseline protocol was amended and re-evaluated at regular intervals. Significant changes for process and outcome measures related to utilization of INO were detected using statistical process control, bivariate analyses using t test or nonparametric Wilcoxon rank-sum test as appropriate, and chi-square and Fisher exact testing as appropriate. Comparisons between the pre-plan-do-study-act group (January 2012 to July 2014) and post-plan-do-study-act group (August 2014 to October 2015) were made.RESULTS: One hundred sixteen INO courses in 95 subjects were administered during the pre-plan-do-study-act period, and 44 episodes were initiated in 39 subjects during the post-plan-do-study-act period. Process control charts demonstrate significant reductions in the percentage of INO doses > 20 ppm and the percentage of prolonged (>4-d) INO courses. Prolonged INO courses decreased from 67.9 to 40% (P = .032), whereas the median duration of INO per course decreased from 8 to 4 d (P < .001). The percentage of INO courses that exceeded the dose of 20 ppm decreased from 18.1 to 2.3% (P = .009). Very delayed INO weaning (weaning at FIO2 ? 0.40) decreased from 41.9 to 21.2% (P = .038). There were no differences in the percentage of INO courses administered to non-sedated subjects or the percentage of INO courses administered to preterm infants. There was no difference for death or ECMO between groups.CONCLUSIONS: Implementation of a shared baseline protocol to encourage appropriate INO initiation and weaning safely decreased INO exposures. Focused efforts on reducing unapproved INO use in preterm infants are warranted.
|
['Administration, Inhalation', 'Bronchodilator Agents', 'Evidence-Based Medicine', 'Extracorporeal Membrane Oxygenation', 'Female', 'Health Plan Implementation', 'Humans', 'Infant, Newborn', 'Infant, Premature', 'Intensive Care Units, Neonatal', 'Male', 'Nitric Oxide', 'Quality Improvement', 'Respiratory Insufficiency', 'Statistics, Nonparametric']
| 28,974,647
|
[['E02.319.267.050'], ['D27.505.696.663.050.110', 'D27.505.954.796.050.100'], ['H02.249.750', 'H02.403.200.400'], ['E02.880.301', 'E04.292.451'], ['N03.349.300'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703.520'], ['M01.060.703.520.520'], ['N02.278.388.493.390.380'], ['D01.339.387', 'D01.625.550.500', 'D01.625.700.500', 'D01.650.550.587.600'], ['J01.293.754', 'N04.761.744'], ['C08.618.846'], ['E05.318.740.995', 'N05.715.360.750.760', 'N06.850.520.830.995']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Disciplines and Occupations [H]', 'Health Care [N]', 'Organisms [B]', 'Named Groups [M]', 'Technology, Industry, and Agriculture [J]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 1
| 1
| 0
|
Potential utility of rhIGF-1 in neuromuscular and/or degenerative disease.
|
Neuromuscular/neurodegenerative disorders, such as the death of spinal cord motor neurons in amyotrophic lateral sclerosis (ALS) or the degeneration of spinal cord motor neuron axons in certain peripheral neuropathies, present a unique opportunity for therapeutic intervention with neurotrophic proteins. We have found that in mixed rat embryonic spinal cord cultures or in purified motor neuron preparations, recombinant human insulin-like growth factor 1 (rhIGF-1) enhances the survival of motor neurons at EC50 concentrations of 2 nM, consistent with an interaction at the tyrosine kinase-coupled rhIGF-1 receptor. In a model of programmed cell death in ovo, administration of rhIGF-1 produces a marked survival of motor neurons. In a variety of models of predominantly motor neuron or nerve injury in rodents, administration of rhIGF-1 prevents the death of motor neurons in neonatal facial nerve lesions, attenuates the loss of cholinergic phenotype in adult hypoglossal nerve axotomy and hastens recovery from sciatic nerve crush in mice. In a genetic model of motor neuron compromise, the wobbler mouse, rhIGF-1 (1 mg/kg s.c. daily) delayed the deterioration of grip strength and provided for a more normal distribution of fibre types. In addition, rhIGF-1 (0.3-1.0 mg/kg s.c. daily) prevents the motor and/or sensory neuropathy in rodents caused by vincristine, cisplatinum or Taxol. These combined data indicate that rhIGF-1 has marked effects on the survival of compromised motor neurons and the maintenance of their axons and functional connections. They also suggest the potential utility of rhIGF-1 for the treatment of diseases such as ALS and certain neuropathies.
|
['Amyotrophic Lateral Sclerosis', 'Animals', 'Cells, Cultured', 'Choline O-Acetyltransferase', 'Disease Models, Animal', 'Female', 'Humans', 'Insulin-Like Growth Factor I', 'Mice', 'Motor Neurons', 'Nerve Degeneration', 'Rats', 'Rats, Sprague-Dawley', 'Recombinant Proteins', 'Spinal Cord']
| 8,866,126
|
[['C10.228.854.139', 'C10.574.562.250', 'C10.574.950.050', 'C10.668.467.250', 'C18.452.845.800.050'], ['B01.050'], ['A11.251'], ['D08.811.913.050.134.180'], ['C22.232', 'E05.598.500', 'E05.599.395.080'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.644.276.937.400', 'D12.776.124.862.400', 'D12.776.467.937.400', 'D23.529.937.400'], ['B01.050.150.900.649.313.992.635.505.500'], ['A08.675.655.500', 'A11.671.655.500'], ['C23.550.737'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.750'], ['D12.776.828'], ['A08.186.854']]
|
['Diseases [C]', 'Organisms [B]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Maternal obesity as a risk factor for early childhood type 1 diabetes: a nationwide, prospective, population-based case-control study.
|
AIMS/HYPOTHESIS: Genetic and environmental factors are believed to cause type 1 diabetes. The aim of this study was to investigate the influence of maternal BMI and gestational weight gain on the subsequent risk of childhood type 1 diabetes.METHODS: Children in the Swedish National Quality Register for Diabetes in Children were matched with control children from the Swedish Medical Birth Register. Children were included whose mothers had data available on BMI in early pregnancy and gestational weight gain, giving a total of 16,179 individuals: 3231 children with type 1 diabetes and 12,948 control children.RESULTS: Mothers of children with type 1 diabetes were more likely to be obese (9% [n = 292/3231] vs 7.7% [n = 991/12,948]; p = 0.02) and/or have diabetes themselves (2.8% [n = 90/3231] vs 0.8% [n = 108/12,948]; p < 0.001) compared with mothers of control children. Gestational weight gain did not differ significantly between the two groups of mothers. In mothers without diabetes, maternal obesity was a significant risk factor for type 1 diabetes in the offspring (p = 0.04). A child had an increased risk of developing type 1 diabetes if the mother had been obese in early pregnancy (crude OR 1.20; 95% CI 1.05, 1.38; adjusted OR 1.18; 95% CI 1.02, 1.36). Among children with type 1 diabetes (n = 3231) there was a difference (p < 0.001) in age at onset in relation to the mother's BMI. Among children in the oldest age group (15-19 years), there were more mothers who had been underweight during pregnancy, while in the youngest age group (0-4 years) the pattern was reversed.CONCLUSIONS/INTERPRETATION: Maternal obesity, in the absence of maternal diabetes, is a risk factor for type 1 diabetes in the offspring, and influences the age of onset of type 1 diabetes. This emphasises the importance of a normal maternal BMI to potentially decrease the incidence of type 1 diabetes.
|
['Adolescent', 'Adult', 'Age of Onset', 'Case-Control Studies', 'Child', 'Child, Preschool', 'Diabetes Mellitus, Type 1', 'Female', 'Humans', 'Infant', 'Male', 'Obesity', 'Prospective Studies', 'Risk Factors', 'Young Adult']
| 29,098,322
|
[['M01.060.057'], ['M01.060.116'], ['N05.715.350.075.100', 'N06.850.490.250.100'], ['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['M01.060.406'], ['M01.060.406.448'], ['C18.452.394.750.124', 'C19.246.267', 'C20.111.327'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703'], ['C18.654.726.500', 'C23.888.144.699.500', 'E01.370.600.115.100.160.120.699.500', 'G07.100.100.160.120.699.500'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Health Care [N]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Organisms [B]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
[Initial clinical experience with a new percutaneous peripheral atherectomy device for the treatment of femoro-popliteal stenoses].
|
BACKGROUND: Evaluation of the efficacy and safety of a new 7F-atherectomy device (30-day endpoint) for the treatment of short and mid-length arterial lesions with a reference diameter of 2.5-7 mm.MATERIAL AND METHODS: Fifty-eight femoto-popliteal stenoses in 46 patients (67% male, mean age 66 +/- 9 years) with chronic peripheral occlusive disease of the lower limbs [Rutherford stage 2: n = 13 (28%); stage 3: n = 29 (63%), stage 4: 2 (4%), stage 5: n = 2 (4%)], were treated with directional atherectomy. Target lesion characteristics: Common femoral artery: n = 1 (2%), superficial femoral artery: n = 47 (81%); popliteal artery, n = 10 (17%); in stent n = 3 (5 %). Thirty (65 %) of the interventions were performed using an antegrade approach, 16 (35%) interventions in cross-over technique. Mean degree of stenosis was 83 +/- 11 mm, mean length of lesion was 37 +/- 37 mm.RESULTS: 6.5 +/- 2 (4-10) passes of the lesion were performed with the catheter. Three lesions were treated after predilatation, 55 (95%) interventions as primary atherectomy. In 31/58 lesions (53%) additional balloon angioplasty was performed, in 1 lesion (2%) additional stent placement was needed. The mean degree of stenosis after atherectomy was reduced to 29 +/- 20% (0-60%) after additional balloon angioplasty, it was 11 +/- 10% (0-30 %). A residual stenosis of < 50% after plain atherectomy was achieved in 55 (95%) lesions, of < 30% in 49 (84%).COMPLICATIONS: 3 (6.5%) cases of embolism of debris were detected and treated successfully by aspiration. The mean ankle-brachial index increased from 0.62 +/- 0.12 to 0.92 +/- 0.36 before discharge, and to 0.86 +/- 0.17 after 30 days. Rutherford stage after 30 days: stage 0: n = 038 (83%); Stage 1: n = 4 (8%); Stage 2: n = 3 (6%); Stage 5: n = 1 (2%).CONCLUSION: Lesions up to 8 cm in length of the femoropopliteal arteries can be treated successfully in most cases with the new atherectomy catheter. Embolism, the only complication that occurred, can be avoided by cleaning the nose cone after at least 4 passes of the lesion.
|
['Aged', 'Angiography', 'Angioplasty, Balloon', 'Arterial Occlusive Diseases', 'Atherectomy', 'Catheterization', 'Data Interpretation, Statistical', 'Female', 'Femoral Artery', 'Follow-Up Studies', 'Humans', 'Intermittent Claudication', 'Leg', 'Male', 'Middle Aged', 'Popliteal Artery', 'Postoperative Care', 'Prospective Studies', 'Recurrence', 'Safety', 'Stents', 'Time Factors', 'Ultrasonography, Doppler', 'Ultrasonography, Doppler, Color']
| 14,712,409
|
[['M01.060.116.100'], ['E01.370.350.700.060', 'E01.370.370.050'], ['E02.148.050.060', 'E04.100.814.529.124.060', 'E04.502.382.124.060', 'E05.157.016.060'], ['C14.907.137'], ['E02.148.050.120', 'E04.100.814.529.124.120', 'E04.502.382.124.120', 'E05.157.016.120'], ['E02.148', 'E05.157'], ['E05.245.380', 'E05.318.740.300', 'L01.313.500.750.190.380', 'N05.715.360.750.300', 'N06.850.520.830.300'], ['A07.015.114.351'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C14.907.137.126.669', 'C23.888.531'], ['A01.378.610.500'], ['M01.060.116.630'], ['A07.015.114.681'], ['E02.760.731.700', 'E04.604.500', 'N02.421.585.722.700'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['C23.550.291.937'], ['N06.850.135.060.075'], ['E07.695.750'], ['G01.910.857'], ['E01.370.350.850.850'], ['E01.370.350.850.850.850.850']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Information Science [L]', 'Health Care [N]', 'Anatomy [A]', 'Organisms [B]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 1
| 1
| 0
|
The impact of pelvic and lower extremity fractures on the incidence of lower extremity deep vein thrombosis in high-risk trauma patients. Winner of the Best Paper Award from the Gold Medal Forum.
|
Lower extremity fractures (LEFx) and pelvic fractures (PFx) are believed to increase the risk of lower extremity deep vein thrombosis (LEDVT). We studied trauma patients at high risk for LEDVT to determine whether an increased incidence of LEDVT was associated with LEFx and/or PFx. From January 1995 through December 1997 4163 trauma patients were admitted to our Level I trauma center. One thousand ninety-three patients at high risk for LEDVT were screened with serial lower extremity venous duplex ultrasound. Their medical records were retrospectively reviewed for demographics, mechanism of injury, and fracture data. The occurrence of LEDVT, pulmonary embolus, and LEDVT prophylaxis and treatment were noted. The incidence of LEDVT in the fracture group (Fx) was compared with that in the nonfracture group (NFx) using chi-square analysis and logistic regression. Statistical significance was set at < or = 0.05. Complete data were available for 1059 of 1093 patients. Five hundred sixty-nine (53.73%) patients had PFx and/or LEFx, 151 (14.26%) patients had PFx only, 317 (29.3%) patients had LEFx only, and 101 (9.54%) patients had both PFx and LEFx. Four hundred ninety (46.27%) patients had NFx. In 1059 patients LEDVT was detected in 125 (11.8%). Sixty-three patients in the Fx groups developed LEDVT (50.4%): 19 (15.2%) PFx patients, 15 (12.0%) PFx/LEFx patients, and 29 (23.2%) LEFx patients. Sixty-two (49.6%) NFx patients developed LEDVT. LEDVT incidence was not significantly different between the Fx and NFx groups or among the PFx, LEFx, and PFx/LEFx groups (P = 0.317). Nine patients developed pulmonary embolism: four NFx patients, two LEFx patients, two PFx patients, and one PFx/LEFx patient. Significant predictors of LEDVT were age and hospital length of stay. Mean age in patients with LEDVT was 47.58 years and in patients without LEDVT it was 40.89 years (P < 0.001). Mean hospital length of stay in patients with LEDVT was 29.81 days and in patients without LEDVT it was 16.84 days. The power of this study to detect differences representing medium effect sizes was greater than 90 per cent. We conclude that LEFx and/or PFx was not associated with an increased incidence of LEDVT in trauma patients at high risk for LEDVT. Lower extremity venous duplex ultrasound needs to be performed in both Fx and NFx groups to detect LEDVTs.
|
['Adolescent', 'Adult', 'Aged', 'Aged, 80 and over', 'Awards and Prizes', 'Female', 'Femoral Fractures', 'Fractures, Bone', 'Humans', 'Incidence', 'Leg Injuries', 'Male', 'Middle Aged', 'Pelvic Bones', 'Retrospective Studies', 'Risk', 'Tibial Fractures', 'Ultrasonography', 'Venous Thrombosis']
| 12,852,501
|
[['M01.060.057'], ['M01.060.116'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['K01.150'], ['C26.404.061', 'C26.558.276'], ['C26.404'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.308.985.525.375', 'N01.224.935.597.500', 'N06.850.505.400.975.525.375', 'N06.850.520.308.985.525.375'], ['C26.558'], ['M01.060.116.630'], ['A02.835.232.043.825'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E05.318.740.600.800', 'G17.680.750', 'N05.715.360.750.625.700', 'N06.850.520.830.600.800'], ['C26.404.875', 'C26.558.857'], ['E01.370.350.850'], ['C14.907.355.830.925']]
|
['Named Groups [M]', 'Humanities [K]', 'Diseases [C]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Anatomy [A]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Terapeutic Potential of Microencapsulated Sertoli Cells in Huntington Disease.
|
INTRODUCTION: Immune dysfunction, promoted by pro-inflammatory cytokines, plays a pivotal role in neurodegeneration associated with Huntington's disease.AIMS: The aim of this study was to investigate the emerging immunoregulatory and antiinflammatory properties of Sertoli cells in Huntington's disease.METHODS: The experimental R6/2 mouse model of Huntington's disease was treated by a single intraperitoneal injection of microencapsulated prepubertal porcine Sertoli cells and lifespan, motor performance and striatal inflammatory pattern have been evaluated.RESULTS: The results of this study demonstrated that a single intraperitoneal injection of microencapsulated prepubertal porcine Sertoli cells uniquely improved performances and extended the life expectancy of R6/2 Huntington's disease mice, by immune dysfunction modulation in brain.CONCLUSIONS: This study highlights the immunomodulatory and trophic role of Sertoli cells that could be of help in the treatment of neurodegenerative disorders.
|
['Animals', 'Animals, Newborn', 'Apoptosis', 'Corpus Striatum', 'Cyclooxygenase 2', 'Cytokines', 'Disease Models, Animal', 'Drug Compounding', 'Female', 'Gene Expression Regulation', 'Humans', 'Huntingtin Protein', 'Huntington Disease', 'Male', 'Mice', 'Mice, Transgenic', 'Motor Activity', 'Nitric Oxide Synthase Type II', 'Sertoli Cells', 'Survival Analysis', 'Swine', 'Trinucleotide Repeats']
| 27,225,886
|
[['B01.050'], ['B01.050.050.282'], ['G04.146.954.035'], ['A08.186.211.200.885.287.249.487'], ['D08.811.600.720.750'], ['D12.644.276.374', 'D12.776.467.374', 'D23.529.374'], ['C22.232', 'E05.598.500', 'E05.599.395.080'], ['E05.916.270'], ['G05.308'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.776.441'], ['C10.228.140.079.545', 'C10.228.140.380.278', 'C10.228.662.262.249.750', 'C10.574.500.497', 'C16.320.400.430', 'F03.615.250.400', 'F03.615.400.390'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.136.500', 'B01.050.150.900.649.313.992.635.505.500.800'], ['F01.145.632', 'G11.427.410.698'], ['D08.811.682.664.500.772.500', 'D12.776.157.687.575', 'D12.776.660.720.575'], ['A05.360.444.849.789', 'A11.382.952', 'A11.436.837'], ['E05.318.740.998', 'N05.715.360.750.795', 'N06.850.520.830.998'], ['B01.050.150.900.649.313.500.880'], ['G02.111.570.080.708.800.500.850', 'G05.360.080.708.800.500.850', 'G05.360.340.024.850.500.850']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Psychiatry and Psychology [F]', 'Health Care [N]']
| 1
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Platelet thromboxane formation capacity after ethanol withdrawal in chronic alcoholics.
|
Collagen-, arachidonate- and ADP-stimulated platelet thromboxane B2 (TXB2) formation was studied in platelet-rich plasma (PRP) of 14 alcoholics, 7 of whom had a biopsy-verified alcoholic fatty liver. On admission for detoxication, the alcoholics showed decreased platelet count and aggregability (p less than 0.001) as compared to nonalcoholic healthy controls. Platelet TXB2 formation was decreased (p less than 0.01), if PRP was stimulated by arachidonate, but not if it was stimulated by ADP or collagen. In contrast, 9-14 days after ethanol withdrawal platelet TXB2 formation had increased to markedly higher levels than those seen in nonalcoholic controls (p less than 0.01), if PRP was stimulated by ADP, but not if it was stimulated by arachidonate or collagen. Skin bleeding time was found to be prolonged (p less than 0.05) on admission in alcoholics having fatty liver, but it normalized within 2 weeks after ethanol withdrawal. We conclude that the effect of ethanol withdrawal in alcoholics on platelet TXB2 formation is influenced by platelet count, aggregability and the agonist used to induce platelet aggregation.
|
['Adenosine Diphosphate', 'Adult', 'Alcoholism', 'Arachidonic Acid', 'Arachidonic Acids', 'Bleeding Time', 'Blood Platelets', 'Collagen', 'Ethanol', 'Female', 'Fibrinogen', 'Humans', 'Liver Diseases, Alcoholic', 'Male', 'Middle Aged', 'Platelet Aggregation', 'Platelet Count', 'Serum Albumin', 'Substance Withdrawal Syndrome', 'Thromboxane B2']
| 3,141,251
|
[['D03.633.100.759.646.138.124', 'D13.695.667.138.124', 'D13.695.827.068.124'], ['M01.060.116'], ['C25.775.100.250', 'F03.900.100.350'], ['D10.251.355.255.100.100', 'D10.251.355.310.166.100'], ['D10.251.355.255.100', 'D10.251.355.310.166'], ['E01.370.225.625.625.100', 'E05.200.625.625.100', 'G09.188.087'], ['A11.118.188', 'A15.145.229.188'], ['D05.750.078.280', 'D12.776.860.300.250'], ['D02.033.375'], ['D12.776.124.050.250', 'D12.776.124.125.500', 'D12.776.811.300', 'D23.119.490'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C06.552.645', 'C25.775.100.087.645'], ['M01.060.116.630'], ['G09.188.370.687', 'G09.188.390.600.640'], ['E01.370.225.500.195.107.740', 'E01.370.225.625.107.700', 'E01.370.225.625.625.625', 'E05.200.500.195.107.740', 'E05.200.625.107.700', 'E05.200.625.625.625', 'E05.242.195.107.740', 'G04.140.107.740', 'G09.188.105.700'], ['D12.776.034.841', 'D12.776.124.727'], ['C25.775.835', 'F03.900.825'], ['D10.251.355.255.100.825.810', 'D10.251.355.310.166.971.810']]
|
['Chemicals and Drugs [D]', 'Named Groups [M]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Organisms [B]']
| 1
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Region specific gene expressions in the central nervous system of the ascidian embryo.
|
The vertebrate brain is regionalized during development into forebrain, midbrain and hindbrain. Fibroblast growth factor 8 (FGF8) is expressed in the midbrain/hindbrain boundary (MHB) and functions as an organizer molecule. Previous studies demonstrated that the brain of basal chordates or ascidians is also regionalized at least into fore/midbrain and hindbrain. To better understand the ascidian brain regionalization, the expression of the Ciona Fgf8/17/18 gene was compared with the expression of Otx, En and Pax2/5/8 genes. The expression pattern of these genes resembled that of the genes in the vertebrate forebrain, midbrain, MHB and hindbrain, each of those domains being characterized by sole or combined expression of Otx, Pax2/5/8, En and Fgf8/17/18. In addition, the putative forebrain and midbrain expressed Ci-FgfL and Ci-Fgf9/16/20, respectively. Therefore, the regionalization of the ascidian larval central nervous system was also marked by the expression of Fgf genes.
|
['Amino Acid Sequence', 'Animals', 'Brain', 'DNA-Binding Proteins', 'Fibroblast Growth Factors', 'Homeodomain Proteins', 'In Situ Hybridization', 'Molecular Sequence Data', 'Nuclear Proteins', 'Otx Transcription Factors', 'PAX2 Transcription Factor', 'PAX5 Transcription Factor', 'Trans-Activators', 'Transcription Factors', 'Urochordata']
| 12,617,820
|
[['G02.111.570.060', 'L01.453.245.667.060'], ['B01.050'], ['A08.186.211'], ['D12.776.260'], ['D12.644.276.624', 'D12.776.467.624', 'D23.529.624'], ['D12.776.260.400'], ['E01.370.225.500.620.670.325', 'E01.370.225.750.600.670.325', 'E05.200.500.620.670.325', 'E05.200.750.600.670.325', 'E05.393.661.475'], ['L01.453.245.667'], ['D12.776.660'], ['D12.776.260.400.718', 'D12.776.930.650'], ['D12.776.260.645.750', 'D12.776.930.700.750'], ['D12.776.260.645.500', 'D12.776.930.700.500'], ['D12.776.260.755', 'D12.776.930.900', 'D12.776.964.925.984'], ['D12.776.930'], ['B01.050.150.200.727', 'B01.050.500.272.727']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Organisms [B]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Slit-lamp fluid-gas exchange and other office procedures following vitreoretinal surgery.
|
Many complicated postoperative vitreoretinal cases require reoperations. It is possible to perform some of these procedures outside the operating room. We describe examining room techniques for fluid-gas exchange, fluid and gas aspiration, and adherent vitreous strand removal. We also discuss a slit-lamp technique for using sodium hyaluronate (Healon) following fluid-gas exchange to eliminate optical distortion from endothelial striae.
|
['Anesthesia, Local', 'Aphakia, Postcataract', 'Cornea', 'Humans', 'Hyaluronic Acid', 'Intraocular Pressure', 'Needles', 'Operating Rooms', 'Postoperative Care', 'Postoperative Complications', 'Posture', 'Retina', 'Suction', 'Vitrectomy']
| 4,015,489
|
[['E03.155.086.231'], ['C11.510.103.110'], ['A09.371.060.217'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D09.698.373.475'], ['G14.440'], ['E07.612'], ['N02.278.388.700'], ['E02.760.731.700', 'E04.604.500', 'N02.421.585.722.700'], ['C23.550.767'], ['G11.427.695'], ['A09.371.729'], ['E04.237.890'], ['E04.540.960']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Anatomy [A]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Health Care [N]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Experimental test of spatial updating models for monkey eye-head gaze shifts.
|
How the brain maintains an accurate and stable representation of visual target locations despite the occurrence of saccadic gaze shifts is a classical problem in oculomotor research. Here we test and dissociate the predictions of different conceptual models for head-unrestrained gaze-localization behavior of macaque monkeys. We adopted the double-step paradigm with rapid eye-head gaze shifts to measure localization accuracy in response to flashed visual stimuli in darkness. We presented the second target flash either before (static), or during (dynamic) the first gaze displacement. In the dynamic case the brief visual flash induced a small retinal streak of up to about 20 deg at an unpredictable moment and retinal location during the eye-head gaze shift, which provides serious challenges for the gaze-control system. However, for both stimulus conditions, monkeys localized the flashed targets with accurate gaze shifts, which rules out several models of visuomotor control. First, these findings exclude the possibility that gaze-shift programming relies on retinal inputs only. Instead, they support the notion that accurate eye-head motor feedback updates the gaze-saccade coordinates. Second, in dynamic trials the visuomotor system cannot rely on the coordinates of the planned first eye-head saccade either, which rules out remapping on the basis of a predictive corollary gaze-displacement signal. Finally, because gaze-related head movements were also goal-directed, requiring continuous access to eye-in-head position, we propose that our results best support a dynamic feedback scheme for spatial updating in which visuomotor control incorporates accurate signals about instantaneous eye- and head positions rather than relative eye- and head displacements.
|
['Animals', 'Biofeedback, Psychology', 'Eye Movements', 'Fixation, Ocular', 'Head Movements', 'Humans', 'Macaca', 'Photic Stimulation', 'Psychomotor Performance', 'Saccades']
| 23,118,883
|
[['B01.050'], ['E02.190.525.123', 'F02.830.131', 'F04.754.137.301', 'F04.754.308.500'], ['G11.427.410.140', 'G14.350'], ['G14.350.253'], ['G11.427.410.478'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['B01.050.150.900.649.313.988.400.112.199.120.510'], ['E05.723.729'], ['F02.808', 'G11.427.700', 'G11.561.660'], ['G14.350.500']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[Image reconstruction in electrical impedance tomography based on genetic algorithm].
|
Image reconstruction in electrical impedance tomography (EIT) is a highly ill-posed, non-linear inverse problem. The modified Newton-Raphson (MNR) iteration algorithm is deduced from the strictest theoretic analysis. It is an optimization algorithm based on minimizing the object function. The MNR algorithm with regularization technique is usually not stable, due to the serious image reconstruction model error and measurement noise. So the reconstruction precision is not high when used in static EIT. A new static image reconstruction method for EIT based on genetic algorithm (GA-EIT) is proposed in this paper. The experimental results indicate that the performance (including stability, the precision and space resolution in reconstructing the static EIT image) of the GA-EIT algorithm is better than that of the MNR algorithm.
|
['Algorithms', 'Electric Impedance', 'Image Processing, Computer-Assisted', 'Nonlinear Dynamics', 'Tomography']
| 12,744,177
|
[['G17.035', 'L01.224.050'], ['G01.358.500.249.277.350'], ['L01.224.308'], ['E05.599.850', 'H01.548.675'], ['E01.370.350.825']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]']
| 0
| 0
| 0
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
|
Cyclooxygenase 2 is induced in colonic epithelial cells in inflammatory bowel disease.
|
BACKGROUND & AIMS: Prostaglandins are synthesized by cyclooxygenases (COX)-1 and -2. The expression and cellular localization of COX-1 and COX-2 in normal human colon and inflammatory bowel disease (IBD) surgical resections were studied.METHODS: COX-1 and COX-2 protein expression and cellular localization were assessed by Western blotting and immunohistochemistry.RESULTS: COX-1 protein was expressed at equal levels in normal, Crohn's disease, and ulcerative colitis colonic epithelial cells. COX-2 protein was not detected in normal epithelial cells but was detected in Crohn's disease and ulcerative colitis epithelial cells. Immunohistochemistry of normal, Crohn's colitis, and ulcerative colitis tissue showed equivalent COX-1 expression in epithelial cells in the lower half of the colonic crypts. COX-2 expression was absent from normal colon, whereas in Crohn's colitis and ulcerative colitis, COX-2 was observed in apical epithelial cells and in lamina propria mononuclear cells. In Crohn's ileitis, COX-2 was present in the villus epithelial cells. In ulcerative colitis, colonic epithelial cells expressing COX-2 also expressed inducible nitric oxide synthase.CONCLUSIONS: COX-1 was localized in the crypt epithelium of the normal ileum and colon, and its expression was unchanged in IBD. COX-2 was undetectable in normal ileum or colon, but it was induced in apical epithelial cells of inflamed foci in IBD.
|
['Adult', 'Aged', 'Aged, 80 and over', 'Colitis', 'Colitis, Ulcerative', 'Colon', 'Crohn Disease', 'Cyclooxygenase 1', 'Cyclooxygenase 2', 'Enzyme Induction', 'Female', 'Humans', 'Ileitis', 'Inflammatory Bowel Diseases', 'Intestinal Mucosa', 'Isoenzymes', 'Male', 'Membrane Proteins', 'Middle Aged', 'Prostaglandin-Endoperoxide Synthases', 'RNA, Messenger', 'Reference Values']
| 9,679,035
|
[['M01.060.116'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['C06.405.205.265', 'C06.405.469.158.188'], ['C06.405.205.265.231', 'C06.405.205.731.249', 'C06.405.469.158.188.231', 'C06.405.469.432.249'], ['A03.556.124.526.356', 'A03.556.249.249.356'], ['C06.405.205.731.500', 'C06.405.469.432.500'], ['D08.811.600.720.500'], ['D08.811.600.720.750'], ['G05.308.320.200'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C06.405.205.462.624', 'C06.405.469.326.875', 'C06.405.469.420.520'], ['C06.405.205.731', 'C06.405.469.432'], ['A03.556.124.369', 'A10.615.550.444'], ['D08.811.348', 'D12.776.800.300'], ['D12.776.543'], ['M01.060.116.630'], ['D08.811.600.720', 'D08.811.682.690.708.715'], ['D13.444.735.544'], ['E05.978.810']]
|
['Named Groups [M]', 'Diseases [C]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Added soluble fiber enhances the satiating power of low-energy-density liquid yogurts.
|
BACKGROUND: Low-energy-density foods with high satiating power may be useful tools for weight management. Energy density of yogurts can range from 0.4 to 1.8 kcal/g.OBJECTIVE: To test the effects of added inulin, a soluble fiber, on the satiating properties of low-energy-density and high-energy-density yogurt beverages (16 oz or 472 mL).DESIGN: The study followed a within-subject preload design with repeated measures. Each participant completed six conditions, presented in a counterbalanced order.SUBJECTS: Participants were 18 men and 20 women, aged 18 to 35 years.INTERVENTION: The experimental conditions were two high-energy-density yogurt beverages (440 kcal; 0.9 kcal/g) and two low-energy-density yogurt beverages (180 kcal; 0.4 kcal/g) with or without inulin (6 g) and an equal volume of orange juice (180 kcal). A no beverage control condition was used as well.MAIN OUTCOME MEASURES: Repeated ratings of hunger, fullness, and desire to eat and energy consumption at the lunch meal served 120 minutes post-ingestion were the main measures.STATISTICAL ANALYSES PERFORMED: Repeated measures analyses of variance were used to analyze motivational ratings and energy and nutrient intakes at the test meal.RESULTS: Yogurt beverages and liquid orange juice significantly suppressed appetite and promoted satiety relatively to the no beverage condition. Yogurt beverages had greater satiating power than did orange juice, as evidenced by higher satiety ratings and reduced energy intakes at lunch. The satiating power of low-energy-density yogurt with inulin was comparable to that of high-energy-density yogurt.CONCLUSIONS: Energy presented in liquid form can have satiating power. Added fiber can potentiate the satiating properties of low-energy-density liquid yogurts. Adding fiber to low-energy-density foods may be an effective way to suppress appetite and control food intake.
|
['Adolescent', 'Adult', 'Analysis of Variance', 'Beverages', 'Citrus sinensis', 'Cross-Over Studies', 'Dietary Fats', 'Dietary Fiber', 'Energy Intake', 'Female', 'Humans', 'Inulin', 'Male', 'Nutritive Value', 'Obesity', 'Satiation', 'Solubility', 'Yogurt', 'Young Adult']
| 19,857,627
|
[['M01.060.057'], ['M01.060.116'], ['E05.318.740.150', 'N05.715.360.750.125', 'N06.850.520.830.150'], ['G07.203.100', 'J02.200'], ['B01.650.940.800.575.912.250.875.177.769'], ['E05.318.370.150', 'N05.715.360.325.150', 'N06.850.520.445.150'], ['D10.212.302', 'G07.203.300.375', 'J02.500.375'], ['D09.301.416', 'G07.203.300.400', 'J02.500.400'], ['G07.203.650.240.340'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D05.750.078.562.855.750', 'D09.301.915.750', 'D09.698.350.500', 'D09.698.365.855.750'], ['G07.203.650.660', 'J01.576.423.850.730.750', 'N06.850.601.750'], ['C18.654.726.500', 'C23.888.144.699.500', 'E01.370.600.115.100.160.120.699.500', 'G07.100.100.160.120.699.500'], ['F02.830.749'], ['G02.805'], ['G07.203.200.500.888', 'G07.203.300.350.300.888', 'J02.350.500.888', 'J02.500.350.300.888'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Technology, Industry, and Agriculture [J]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Psychiatry and Psychology [F]']
| 0
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 1
| 0
|
Case management decision support tools: predictive risk report or health risk assessment?
|
It is unclear whether health risk assessment (HRA) or claims-based risk modeling is a superior indicator of the need for case management in Medicaid adults with disabilities. This is a prospective cohort study designed to compare the use of a claims-based Predictive Risk Report (PRR) to a HRA in a Medicaid Supplemental Security Income managed care population. Both the claims-based risk scores and HRAs proved to be significant predictors of case management placement and subsequent emergency department and hospital utilization. The PRR-derived risk scores, however, could be obtained on virtually all enrollees at the time of enrollment, while HRA scores were obtained on only 54% of enrollees by 210 days of enrollment. Furthermore, case management reduced the risk of emergency and hospital utilization. We conclude that the PRR and HRA are equally reliable predictors of need for case management. The PRR has the advantage of earlier availability and of being easier to obtain.
|
['Case Management', 'Cohort Studies', 'Decision Support Systems, Management', 'Health Status Indicators', 'Humans', 'Interviews as Topic', 'Medicaid', 'Prospective Studies', 'Risk Assessment', 'Surveys and Questionnaires', 'United States']
| 18,942,923
|
[['N04.590.233.624.250'], ['E05.318.372.500.750', 'N05.715.360.330.500.750', 'N06.850.520.450.500.750'], ['N04.452.515.135'], ['E05.318.308.980.438.475', 'N05.715.360.300.800.438.375', 'N06.850.520.308.980.438.475'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.308.420', 'L01.399.250.520', 'N05.715.360.300.400', 'N06.850.520.308.420'], ['N03.219.521.346.506.564.655', 'N03.706.615.693'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['E05.318.740.600.800.715', 'N04.452.871.715', 'N05.715.360.750.625.700.690', 'N06.850.505.715', 'N06.850.520.830.600.800.715'], ['E05.318.308.980', 'N05.715.360.300.800', 'N06.850.520.308.980'], ['Z01.107.567.875']]
|
['Health Care [N]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Information Science [L]', 'Geographicals [Z]']
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 1
| 1
|
Spatial arrangement of intra-nucleolar rDNA chromatin in amplified Xenopus oocyte nucleoli: structural changes precede the onset of rDNA transcription.
|
Amphibian oocyte nucleoli are a particular suited object for research on nucleolar chromatin organization. By selective rDNA amplification each pachytene oocyte nucleus accumulates 30 pg of extrachromosomal rDNA, this amount corresponds to 2 million rDNA copies. Following the selective amplification stage, the amplified gene copies are finally distributed within more than thousand extrachromosomal nucleoli per individual oocyte nucleus. The aim of the present study was first to obtain a precise documentation of the fate of amplified rDNA during early Xenopus oogenesis until the final functional integration of these copies into individual oocyte nucleoli, and, second, a close correlation of the structural data with determination of rDNA transcription rates by S1 transcript analysis for the subsequent stages of oocyte differentiation. In order to investigate the structural complexity of the intranuclear rDNA translocation process in detail, a confocal laser scan microscope (CLSM) was used, equipped with an external UV-laser. This instrumentation unambiguously allowed (i) the detection of small clusters of rDNA copies and (ii) the precise spatial documentation of the intranuclear position of rDNA clusters in relation to the protein-free pre-nucleolar protein bodies, a specific characteristic of late pachytene/early diplotene amphibian oocyte nuclei. Our results indicate that the major rDNA translocation processes, e.g. the association of rDNA clusters with pre-nucleolar protein bodies, the formation of ribbon-like pre-nucleolar units sensu Van Gansen and Schramm (J. Cell Sci. 10: 339-367, 1972), and, finally, the translocation of fused rDNA units into the interior of pre-nucleolar protein bodies, occur--for the most part--in absence of massive rDNA transcription. As shown by the S1 transcript analysis, the onset of massive rDNA transcription starts concomitantly with an unraveling of the densely packed rDNA clusters into finely dispersed rDNA units, which were shown by CLSM analysis to be distributed throughout the entire nucleolar volume.
|
['Animals', 'Cell Nucleolus', 'Chromatin', 'DNA, Ribosomal', 'Female', 'Kinetics', 'Microscopy, Electron', 'Oocytes', 'Oogenesis', 'Transcription, Genetic', 'Xenopus laevis']
| 8,735,937
|
[['B01.050'], ['A11.284.430.106.279.345.175'], ['A11.284.430.106.279.345.190.160.180', 'D12.776.664.224', 'G05.360.160.180'], ['D13.444.308.475'], ['G01.374.661', 'G02.111.490'], ['E01.370.350.515.402', 'E05.595.402'], ['A05.360.490.690.680', 'A11.497.497.600'], ['G04.152.650.249', 'G08.686.784.310.500'], ['G02.111.873', 'G05.297.700'], ['B01.050.150.900.090.180.610.500.562']]
|
['Organisms [B]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[Molecular typing of the pathogenic Yersinia enterocolitica strains with pulsed field gel electrophores isolated in China].
|
OBJECTIVE: To investigate the epidemiological and molecular typing features of the pathogenic Yersinia enterocolitica strains isolated in China,using pulsed field gel electrophoresis(PFGE) and standardized PFGE method as well as typing database of Yersinia enterocolitica.METHODS: PFGE analysis was performed as Laboratory Directions for molecular subtyping of Salmonella by PFGE (PulseNet,USA) with some modifications and the results of PFGE were analyzed by BioNumerics soft (Version 4.0, Applied Maths BVBA, Belium).RESULTS: 114 O:3 Yersinia enterocolitica strains were typed by 25 patterns to have found that K6GN11C30012 (50 strains), K6GN11C30015(19 strains) and K6GN11C30016(10 strains) were the major patterns. K6GNllC30012 had 92.2% cluster similarity with K6GN11C30009-K6GN11C30023. This clone included 91.23% strains of 114 0:3 Yersinia enterocolitica strains. 51 0:9 Yersinia enterocolitica strains were typed by 14 patterns; K6GN11C90004 (22 strains) and K6GN11C90010 (13 strains)were the major patterns. K6GN11C90004 had 81.8% cluster similarity with K6GN11C90010 patterns. The major patterns of 0:3 and 0:9 serotypes were quite different.CONCLUSION: O:3 Yersinia enterocolitica strains might originate from the same clone and had very few variation in different years and provinces but O:9 Yersinia enterocolitica strains from two different clones with some changes.
|
['China', 'Electrophoresis, Gel, Pulsed-Field', 'Humans', 'Yersinia enterocolitica']
| 17,172,107
|
[['Z01.252.474.164'], ['E05.196.401.220', 'E05.301.300.220'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['B03.440.450.425.900.300', 'B03.660.250.150.950.160']]
|
['Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]']
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
|
CT scan cerebral hemispheric asymmetries: predictors of recovery from aphasia.
|
Individual variations in anatomic cerebral asymmetries have been linked with specific neurodevelopmental processes, with patterns of cognitive ability, and with recovery from focal brain damage. The present study investigated relationships between cerebral asymmetries and recovery from aphasia. Aphasic patients (N = 25) were assessed for language recovery for 1 year poststroke, and linear measurements of cerebral asymmetries were performed on CT scans. Increasing left occipital width asymmetry was associated with faster rate of language recovery and with higher final language scores during the first year poststroke. There was, moreover, a tendency for increasing left occipital width asymmetry to be associated with less initial impairment. It is hypothesized that those aspects of neural organization conferring better premorbid language skills are the same factors conferring greater recovery of language skills and that occipital width asymmetry serves as a marker for such individual differences in neural organization.
|
['Aged', 'Aged, 80 and over', 'Aphasia', 'Cerebrovascular Disorders', 'Functional Laterality', 'Humans', 'Language', 'Male', 'Middle Aged', 'Tomography, X-Ray Computed']
| 8,491,845
|
[['M01.060.116.100'], ['M01.060.116.100.080'], ['C10.597.606.150.500.800.100', 'C23.888.592.604.150.500.800.100'], ['C10.228.140.300', 'C14.907.253'], ['F02.830.297.425', 'G11.561.225.425'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F01.145.209.399', 'L01.559'], ['M01.060.116.630'], ['E01.370.350.350.810', 'E01.370.350.600.350.700.810', 'E01.370.350.700.700.810', 'E01.370.350.700.810.810', 'E01.370.350.825.810.810']]
|
['Named Groups [M]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 1
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 1
| 1
| 0
| 0
|
Ganglion cells and retinopetal fibers of the larval lamprey retina: an HRP ultrastructural study.
|
The ultrastructure of ganglion cells and centrifugal fibers of the larval lamprey retinas were studied using horseradish peroxidase (HRP) as a marker. Larval ganglion cells were found both in the inner nuclear layer and the inner plexiform layer of the differentiated retina, and also were present in the undifferentiated retina. Direct photoreceptor-ganglion cell contacts and the presence of centrifugal fibers are described for the first time in the lamprey. The centrifugal fibers contact directly with ganglion cells in this species.
|
['Animals', 'Brain', 'Efferent Pathways', 'Fishes', 'Horseradish Peroxidase', 'Lampreys', 'Larva', 'Retina', 'Retinal Ganglion Cells', 'Visual Pathways']
| 2,586,814
|
[['B01.050'], ['A08.186.211'], ['A08.612.380'], ['B01.050.150.900.493'], ['D08.811.682.732.512'], ['B01.050.150.900.493.559'], ['B05.500.500', 'G07.345.500.550.500.500'], ['A09.371.729'], ['A08.675.650.850.875', 'A09.371.729.831.875', 'A11.671.650.850.875'], ['A08.612.220.860']]
|
['Organisms [B]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Adolescent and adult differences in major depression symptom profiles.
|
BACKGROUND: Depression is the leading global cause of disability and often begins in adolescence. The genetic architecture and treatment response profiles for adults and adolescents differ even though identical criteria are used to diagnose depression across different age groups. There is no clear consensus on how these groups differ in their symptom profiles.METHODS: Using data from a two-generation family study, we compared the presentation of DSM-IV depressive symptoms in adolescents and adults with MDD (Major Depressive Disorder). We also compared DSM-IV depressive symptom counts using latent class analysis.RESULTS: Vegetative symptoms (appetite and weight change, loss of energy and insomnia) were more common in adolescent MDD than adult MDD. Anhedonia/loss of interest and concentration problems were more common in adults with MDD. When using latent class analysis to look at depressive symptoms, a vegetative symptom profile was also seen in adolescent depression only.LIMITATIONS: Adults and adolescents were recruited in different ways. Adolescent cases were more likely to be first-onset while adult cases were recurrences. It was not possible to examine how recurrence affected adolescent depression symptom profiles.CONCLUSION: Differences in how depression presents in adolescents and adults may be consistent with different pathophysiological mechanisms. For adolescents, we found that vegetative/physical disturbances were common (loss of energy, changes in weight, appetite and sleep changes). For adults, anhedonia/loss of interest and concentration difficulties were more common.
|
['Adolescent', 'Adolescent Behavior', 'Adult', 'Child', 'Depression', 'Depressive Disorder, Major', 'Diagnostic and Statistical Manual of Mental Disorders', 'Female', 'Humans', 'Male', 'Middle Aged', 'Recurrence']
| 30,243,197
|
[['M01.060.057'], ['F01.145.022'], ['M01.060.116'], ['M01.060.406'], ['F01.145.126.350'], ['F03.600.300.375'], ['L01.453.245.945.200'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['C23.550.291.937']]
|
['Named Groups [M]', 'Psychiatry and Psychology [F]', 'Information Science [L]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
| 0
|
Application of HSV colour system in identification by colour of biological objects on the basis of microscopic images.
|
This paper demonstrates preliminary image processing with the aim of obtaining invariant signs for identification by colour. For this purpose we have used microscopic images of cell structures in coloured peripheral blood smears. The main parameter for the identification is the colouring of the respective cell structures on the basis of which we have created histograms by hue for the available cell types.
|
['Algorithms', 'Basophils', 'Blood Cells', 'Color', 'Coloring Agents', 'Eosinophils', 'Erythrocytes', 'Humans', 'Image Processing, Computer-Assisted', 'Leukocytes', 'Microscopy', 'Monocytes', 'Neutrophils', 'Pattern Recognition, Automated']
| 9,007,363
|
[['G17.035', 'L01.224.050'], ['A11.118.637.415.120', 'A11.627.340.120', 'A15.145.229.637.415.120', 'A15.382.490.315.120'], ['A11.118', 'A15.145.229'], ['G01.590.540.199'], ['D27.720.233'], ['A11.118.637.415.345', 'A11.627.340.345', 'A15.145.229.637.415.345', 'A15.382.490.315.251'], ['A11.118.290', 'A11.443.240', 'A15.145.229.334'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['L01.224.308'], ['A11.118.637', 'A15.145.229.637', 'A15.382.490'], ['E01.370.350.515', 'E05.595', 'H01.671.617.562'], ['A11.118.637.555.652', 'A11.148.580', 'A11.627.624', 'A11.733.547', 'A15.145.229.637.555.652', 'A15.378.316.580', 'A15.382.490.555.652', 'A15.382.670.547', 'A15.382.680.547'], ['A11.118.637.415.583', 'A11.627.340.583', 'A11.733.689', 'A15.145.229.637.415.583', 'A15.382.490.315.583', 'A15.382.680.689'], ['L01.399.750']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
|
[Resection of a Posterior Mediastinal Tumor Using Intraoperative Motor Evoked Potential Monitoring;Report of a Case].
|
A 20-year-old man with a posterior mediastinal tumor incidentally found on a chest X-ray was referred to our hospital. Chest computed tomography showed a 3 cm nodule located on the left side of the 10-11th thoracic vertebra, where the artery of Adamkiewicz is presumed to arise. He underwent left thoracotomy to remove the lesion. The tumor was safely resected with the assistance of intraoperative motor evoked potential(MEP) monitoring. The postoperative diagnosis was a benign schwannoma. In thoracic surgery for posterior mediastinal tumors, intraoperative MEP monitoring is useful for preventing paraplegia.
|
['Evoked Potentials, Motor', 'Humans', 'Magnetic Resonance Imaging', 'Male', 'Mediastinal Neoplasms', 'Monitoring, Intraoperative', 'Multimodal Imaging', 'Tomography, X-Ray Computed', 'Young Adult']
| 28,790,251
|
[['G07.265.216.500.385', 'G11.561.200.500.385'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.350.825.500'], ['C04.588.894.479', 'C08.846.187.580'], ['E01.370.520.510', 'E04.510'], ['E01.370.350.567'], ['E01.370.350.350.810', 'E01.370.350.600.350.700.810', 'E01.370.350.700.700.810', 'E01.370.350.700.810.810', 'E01.370.350.825.810.810'], ['M01.060.116.815']]
|
['Phenomena and Processes [G]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Named Groups [M]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Internal sclerostomy with the Er:YAG laser using a gradient-index (GRIN) endoscope.
|
BACKGROUND AND OBJECTIVE: To show that sclerostomy, a glaucoma filtering surgery, can be performed using an Er:YAG laser. Scarring at the filtering site, a recurrent problem, may be reduced through proper positioning of the sclerostomy by using an intraocular endoscope.MATERIALS AND METHODS: Ab interno full-thickness sclerostomies were performed on eye bank eyes with an Er:YAG laser through a custom made optical delivery system. The intraocular laser probe consisted of a low OH silica fiber inserted in a metallic tapered sheathing. A rigid intraocular endoscope based on gradient-index lenses allowed visualization of the filtration site.RESULTS: A clear view of the anterior chamber angle was obtained through the endoscope, allowing for precise location of the sclerostomy. Full-thickness sclerostomies could then be performed at the desired location. Histologic sections showed thermal necrosis less than 50 microm thick in tissue adjacent to the sclerostomy.CONCLUSIONS: A sclerostomy performed with a combined procedure using an Er:YAG laser and intraocular endoscopy increases the speed of the procedure. The use of a high-resolution intraocular endoscope may increase the success rate of ab interno laser glaucoma surgeries.
|
['Endoscopy', 'Eye Banks', 'Humans', 'Laser Therapy', 'Sclera', 'Sclerostomy']
| 12,027,101
|
[['E01.370.388.250', 'E04.502.250'], ['N02.278.065.900.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.594', 'E04.014.520'], ['A09.371.784'], ['E04.540.450.600', 'E04.579.895']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Anatomy [A]']
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Anti-inflammatory Dimeric 2-(2-Phenylethyl)chromones from the Resinous Wood of Aquilaria sinensis.
|
Sixteen new 2-(2-phenylethyl)chromone dimers, including four pairs of enantiomers (1a/1b, 3a/3b, 6a/6b, and 8a/8b), along with eight optically pure analogues (2, 4, 5, 7, and 9-12) were isolated from the resinous wood of Aquilaria sinensis. Their structures were determined by extensive spectroscopic analysis (1D and 2D NMR, UV, IR, and HRMS) and experimental and computed ECD data. Compounds 1-10 feature an unusual 3,4-dihydro-2 H-pyran ring linkage connecting two 2-(2-phenylethyl)chromone monomeric units, while compounds 11 and 12 possess an unprecedented 6,7-dihydro-5 H-1,4-dioxepine moiety in their structures. A putative biosynthetic pathway of the representative structures via a diepoxy derivative of a chromone with a nonoxygenated A-ring is also proposed. Compounds 1a/1b, 2, 3a/3b, 5, 7, 8a/8b, and 10-12 exhibited significant inhibition of nitric oxide production in lipopolysaccharide-stimulated RAW264.7 cells with IC50 values in the range 7.0-12.0 ìM.
|
['Animals', 'Anti-Inflammatory Agents', 'Cell Line', 'Cholinesterase Inhibitors', 'Flavonoids', 'Lipopolysaccharides', 'Magnetic Resonance Spectroscopy', 'Mice', 'Nitric Oxide', 'RAW 264.7 Cells', 'Resins, Plant', 'Thymelaeaceae', 'Wood']
| 29,227,647
|
[['B01.050'], ['D27.505.954.158'], ['A11.251.210'], ['D27.505.519.389.275', 'D27.505.519.625.120.300', 'D27.505.696.577.120.300'], ['D03.383.663.283.266.450', 'D03.633.100.150.266.450'], ['D09.400.500', 'D09.698.718.450', 'D10.494', 'D23.050.161.616.525', 'D23.946.123.329.500'], ['E05.196.867.519'], ['B01.050.150.900.649.313.992.635.505.500'], ['D01.339.387', 'D01.625.550.500', 'D01.625.700.500', 'D01.650.550.587.600'], ['A11.251.210.172.875', 'A11.733.397.815'], ['D05.750.078.840', 'D20.215.721.500'], ['B01.650.940.800.575.912.250.932'], ['A18.450.500.500', 'J01.637.241.900']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Technology, Industry, and Agriculture [J]']
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
The Belgrade childhood diabetes study - comparison of children with type 1 diabetes with their siblings.
|
A case-control study was conducted in Belgrade (about 320,000 inhabitants 0-16 years old) during the period 1994-97, comprising 68 diabetic children (cases) and 68 controls chosen from the siblings of the cases. Analysis using multivariable logistic regression analysis indicated the following independent risk factors for Type 1 diabetes: higher birth order, infections during the 6 months preceding the onset of the disease and stressful events. Out of individual stressful and psychological factors, 'other' stressful events (severe accident or hospitalisation or death of a close friend, conflict with a teacher, death of a pet, failure in competition, quarrel between parents, punishment, physical attack, war in republics of former Yugoslavia and near drowning in the pool) and learning problems were independent risk factor for Type 1 diabetes. The results obtained in this study of siblings supports the hypothesis that environmental factors play a role in the development of Type 1 diabetes.
|
['Adolescent', 'Age Distribution', 'Birth Order', 'Case-Control Studies', 'Child', 'Child, Preschool', 'Communicable Diseases', 'Diabetes Mellitus, Type 1', 'Female', 'Humans', 'Infant', 'Learning Disabilities', 'Male', 'Models, Statistical', 'Risk Factors', 'Sex Distribution', 'Siblings', 'Stress, Psychological', 'Yugoslavia']
| 16,629,698
|
[['M01.060.057'], ['I01.240.050', 'N01.224.033', 'N06.850.505.400.050'], ['F01.829.263.132', 'I01.240.361.160', 'N01.224.361.160', 'N06.850.505.400.400.160'], ['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['M01.060.406'], ['M01.060.406.448'], ['C01.221', 'C23.550.291.531'], ['C18.452.394.750.124', 'C19.246.267', 'C20.111.327'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703'], ['C10.597.606.150.550', 'C23.888.592.604.150.550', 'F03.625.562'], ['E05.318.740.500', 'E05.599.835', 'N05.715.360.750.530', 'N06.850.520.830.500'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['I01.240.800', 'N01.224.803', 'N06.850.505.400.850'], ['F01.829.263.500.490', 'I01.880.853.150.500.505', 'M01.781'], ['F01.145.126.990', 'F02.830.900'], ['Z01.586.980']]
|
['Named Groups [M]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]', 'Psychiatry and Psychology [F]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Organisms [B]', 'Geographicals [Z]']
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 1
|
Distribution of transferrin and transferrin receptors in the rabbit placenta.
|
The quantity and distribution of transferrin and transferrin-binding sites in the placenta were investigated in rabbits on the 28th-29th days of pregnancy. The animals were injected intravenously with a mixture of 59Fe-125I-labelled rabbit diferric transferrin and 131I-labelled rabbit albumin. The binding of transferrin to placentas removed 3-75 min later was determined by using the 131I-labelled albumin values to correct for tissue content of plasma. Mean values for transferrin binding of 1460 and 560 micrograms/g tissue were obtained 3-15 and 45-75 min after injection, respectively. Gel filtration of placental extracts prepared with the non-ionic detergent, Teric 12A9, showed that the 125I-labelled transferrin bound to a large molecular weight component which had the properties of a specific receptor. The receptor had a higher affinity for diferric transferrin than for apotransferrin. The subcellular distribution of transferrin binding sites was determined by differential centrifugation of placental homogenates and by electron microscope autoradiography. The results with the former method indicated that the transferrin was bound to the microsomal fraction of the cells. Autoradiography showed that the majority of the transferrin molecules were at intracellular sites, mainly on the membrane of intracellular vesicles. It is concluded that iron-containing transferrin molecules enter the trophoblast cells by endocytosis or via a canalicular system after binding to cell membrane receptors. The higher affinity of the receptors for diferric transferrin than for apotransferrin explains the difference in amount of transferrin binding found within 15 min of injecting labelled diferric transferrin and that found 45-75 min later when much of the iron had been removed from the transferrin.
|
['Animals', 'Autoradiography', 'Female', 'Iron', 'Placenta', 'Pregnancy', 'Rabbits', 'Receptors, Cell Surface', 'Receptors, Transferrin', 'Transferrin']
| 6,310,669
|
[['B01.050'], ['E01.370.225.750.132', 'E05.200.750.132', 'E05.799.256'], ['D01.268.556.412', 'D01.268.956.287', 'D01.552.544.412'], ['A16.710'], ['G08.686.784.769'], ['B01.050.150.900.649.313.968.700'], ['D12.776.543.750'], ['D12.776.157.905.500', 'D12.776.543.750.800'], ['D12.776.124.050.800', 'D12.776.124.790.223.839', 'D12.776.157.427.750.500', 'D12.776.377.715.182.839', 'D12.776.556.579.750.500']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
A 100-year perspective on gastrointestinal motility.
|
This contribution to the centennial commemorative issue of the American Journal of Physiology: Gastrointestinal and Liver Physiology identifies some of the important studies of spontaneous electrical and motor activity in the gastrointestinal tract published in the Journal between 1898 and 1996. Emphasis is given to the contributions made by Walter B. Cannon, Walter C. Alvarez, Emil Bozler, C. Ladd Prosser, and James Christensen.
|
['Animals', 'Gastrointestinal Motility', 'History, 19th Century', 'History, 20th Century', 'Periodicals as Topic', 'Peristalsis', 'Physiology']
| 9,565,541
|
[['B01.050'], ['G10.261.360'], ['K01.400.504.937'], ['K01.400.504.968'], ['L01.178.682.829.678'], ['G10.261.360.596'], ['H01.158.782']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Humanities [K]', 'Information Science [L]', 'Disciplines and Occupations [H]']
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
|
Phospholipases. III. Effects of ionic surfactants on the phospholipase-catalyzed hydrolysis of unsonicated egg lecithin liposomes.
|
Apparent values of Km and Vmax have been measured for catalysis of hydrolysis of unsonicated egg lecithin liposomes, activated through addition of 0.4 M n-hexanol, by phospholipases A2 from bee and snake venoms and by phospholipase C from Clostridium welchii as a function of the concentration of three surfactants: hexadecylamine, hexadecyltrimethylammonium bromide, and dihexadecyl phosphate. For all three enzymes, values of Km and Vmax show little or no dependence on the concentration of these ionic surfactants, demonstrating that the liposomal surface charge is not a crucial factor in determining susceptibility to phospholipase-catalyzed hydrolysis.
|
['Amines', 'Cetrimonium Compounds', 'Hexanols', 'Hydrogen-Ion Concentration', 'Kinetics', 'Liposomes', 'Organophosphorus Compounds', 'Phosphatidylcholines', 'Phospholipases', 'Surface-Active Agents']
| 506
|
[['D02.092'], ['D02.092.877.883.111', 'D02.675.276.190'], ['D02.033.415.510', 'D10.289.510'], ['G02.300'], ['G01.374.661', 'G02.111.490'], ['D25.479.517', 'D26.255.260.517', 'J01.637.051.479.517', 'J01.637.087.500.517'], ['D02.705'], ['D10.570.755.375.760.400.800'], ['D08.811.277.352.100.680', 'D08.811.277.352.640.700'], ['D27.720.877']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Technology, Industry, and Agriculture [J]']
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
HLA-DQA1 allele and suballele typing using noncoding sequence polymorphisms. Application to 4AOHW cell panel typing.
|
HLA-DQA1 typing of the 4AOHW cell panel is presented using a novel strategy that exploits both intron and exon polymorphisms. Intron sequences adjacent to the variable HLA-DQA1 second exon exhibit stable polymorphisms that are specific for locus alleles and certain suballelic DR/DQ haplotypes. A PCR-RFLP method has been developed that is based on amplification of a 780-bp segment extending from intron 1 through exon 2 to intron 2. Stable sequence polymorphisms provide restriction enzyme sites and confer mobility variations detected on polyacrylamide minigel electrophoresis. Direct band comparison of amplified products and restriction fragments with known standards facilitates pattern comparison, obviating the requirement for accurate molecular weight determination. This method, using only two enzymes, identifies a total of 11 allelic and suballelic groups, including all eight DQA1 alleles encoded at the second exon.
|
['Alleles', 'Base Sequence', 'Cell Line, Transformed', 'Deoxyribonucleases, Type II Site-Specific', 'Exons', 'HLA-DQ Antigens', 'HLA-DQ alpha-Chains', 'HLA-DR Antigens', 'Haplotypes', 'Histocompatibility Testing', 'Humans', 'Introns', 'Molecular Sequence Data', 'Polymerase Chain Reaction', 'Polymorphism, Restriction Fragment Length']
| 7,905,870
|
[['G05.360.340.024.340.030'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['A11.251.210.172'], ['D08.811.150.280.260', 'D08.811.277.352.335.350.300.260', 'D08.811.277.352.355.325.300.260'], ['G05.360.340.024.340.137.232'], ['D12.776.395.550.509.400.430', 'D12.776.543.550.440.400.430', 'D23.050.301.500.400.400.430', 'D23.050.301.500.450.400.430', 'D23.050.705.552.410.400.430', 'D23.050.705.552.450.400.430'], ['D12.776.395.550.509.400.430.500', 'D12.776.543.550.440.400.430.500', 'D23.050.301.500.400.400.430.500', 'D23.050.301.500.450.400.430.500', 'D23.050.705.552.410.400.430.500', 'D23.050.705.552.450.400.430.500'], ['D12.776.395.550.509.400.440', 'D12.776.543.550.440.400.440', 'D23.050.301.500.400.400.440', 'D23.050.301.500.450.400.440', 'D23.050.705.552.410.400.440', 'D23.050.705.552.450.400.440'], ['G05.380.360'], ['E01.370.225.812.385', 'E05.200.812.385', 'E05.478.594.385'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G05.360.340.024.220.400', 'G05.360.340.024.340.137.515'], ['L01.453.245.667'], ['E05.393.620.500'], ['G05.365.795.595']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
[Characterization of the adaptive response to the action of gamma-rays, induced by low doses of 14C in Chinese hamster fibroblasts].
|
It has been studied the correlation of the mitotic activity of the chromosome aberrations and apoptosis, in the V-79 cells pre-exposure to an adapting dose of ionizing radiation from 14C-thymidine prior to an acute challenge dose of gamma-rays. In spite of that the incubation of the cells with isotope increased of the yield of the chromosome aberrations, but the cells became more resistant to following gamma-irradiation. Increasing the adaptive dose of the 14C on degree didn't influence on the present of the adaptive response. However, using concentrations of the 14C damaged metaphase/anaphase transition and cells blocked in this check-point by apoptotic death. The results suggest, that the cellular selection has been involved in 14C-induced adaptive response, estimated by level of asymmetric chromosome aberrations in V-79 cells.
|
['Adaptation, Physiological', 'Anaphase', 'Animals', 'Carbon Radioisotopes', 'Cell Line', 'Chromosome Aberrations', 'Cricetinae', 'Cricetulus', 'Fibroblasts', 'Gamma Rays', 'Metaphase', 'Mitosis']
| 9,876,490
|
[['G07.025', 'G16.012.500'], ['G04.144.220.220.687.222', 'G04.144.220.220.781.050', 'G05.113.220.687.250', 'G05.113.220.781.050'], ['B01.050'], ['D01.268.150.075.328', 'D01.496.123.328', 'D01.496.749.154'], ['A11.251.210'], ['C23.550.210', 'G05.365.590.175'], ['B01.050.150.900.649.313.992.635.075.250'], ['B01.050.150.900.649.313.992.635.075.250.250'], ['A11.329.228'], ['G01.358.500.505.300', 'G01.750.250.300', 'G01.750.750.400'], ['G04.144.220.220.687.625', 'G04.144.220.220.781.625', 'G05.113.220.687.625', 'G05.113.220.781.625'], ['G04.144.220.220.781', 'G05.113.220.781']]
|
['Phenomena and Processes [G]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Proton-Induced Conformational and Hydration Dynamics in the Influenza A M2 Channel.
|
The influenza A M2 protein is an acid-activated proton channel responsible for acidification of the inside of the virus, a critical step in the viral life cycle. This channel has four central histidine residues that form an acid-activated gate, binding protons from the outside until an activated state allows proton transport to the inside. While previous work has focused on proton transport through the channel, the structural and dynamic changes that accompany proton flux and enable activation have yet to be resolved. In this study, extensive Multiscale Reactive Molecular Dynamics simulations with explicit Grotthuss-shuttling hydrated excess protons are used to explore detailed molecular-level interactions that accompany proton transport in the +0, + 1, and +2 histidine charge states. The results demonstrate how the hydrated excess proton strongly influences both the protein and water hydrogen-bonding network throughout the channel, providing further insight into the channel's acid-activation mechanism and rectification behavior. We find that the excess proton dynamically, as a function of location, shifts the protein structure away from its equilibrium distributions uniquely for different pH conditions consistent with acid-activation. The proton distribution in the xy-plane is also shown to be asymmetric about the channel's main axis, which has potentially important implications for the mechanism of proton conduction and future drug design efforts.
|
['Histidine', 'Humans', 'Hydrogen Bonding', 'Hydrogen-Ion Concentration', 'Molecular Dynamics Simulation', 'Protein Conformation', 'Protons', 'Viral Matrix Proteins', 'Water']
| 31,264,413
|
[['D12.125.072.329', 'D12.125.142.308'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G02.282'], ['G02.300'], ['E05.599.595.500', 'G02.111.570.895', 'L01.224.160.500'], ['G02.111.570.820.709'], ['D01.248.497.300.459.700', 'D01.268.406.750', 'D01.362.340.750', 'G01.249.660.500'], ['D12.776.964.970.880.940'], ['D01.045.250.875', 'D01.248.497.158.459.650', 'D01.650.550.925']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Information Science [L]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Nucleolar introns from Physarum flavicomum contain insertion elements that may explain how mobile group I introns gained their open reading frames.
|
Comparison of two group I intron sequences in the nucleolar genome of the myxomycete Physarum flavicomum to their homologs in the closely related Physarum polycephalum revealed insertion-like elements. One of the insertion-like elements consists of two repetitive sequence motifs of 11 and 101 bp in five and three copies, respectively. The smaller motif, which flanks the larger, resembles a target duplication and indicates a relationship to transposons or retroelements. The insertion-like elements are found in the peripheral loops of the RNA structure; the positions occupied by the ORFs of mobile nucleolar group I introns. The P. flavicomum introns are 1184 and 637 bp in size, located in the large subunit ribosomal RNA gene, and can be folded into group I intron structures at the RNA level. However, the intron 2s from both P. flavicomum and P. polycephalum contain an unusual core region that lacks the P8 segment. None of the introns are able to self-splice in vitro. Southern analysis of different isolates indicates that the introns are not optional in myxomycetes.
|
['Animals', 'Base Sequence', 'Cell Nucleolus', 'Cloning, Molecular', 'DNA Transposable Elements', 'DNA, Fungal', 'DNA, Ribosomal', 'Introns', 'Molecular Sequence Data', 'Nucleic Acid Conformation', 'Open Reading Frames', 'Physarum', 'RNA, Fungal', 'RNA, Ribosomal', 'Repetitive Sequences, Nucleic Acid', 'Sequence Alignment', 'Sequence Analysis, DNA']
| 7,984,404
|
[['B01.050'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['A11.284.430.106.279.345.175'], ['E05.393.220'], ['D13.444.308.520', 'G02.111.570.080.708.330.200', 'G05.360.080.708.330.200', 'G05.360.340.024.425.200'], ['D13.444.308.300'], ['D13.444.308.475'], ['G05.360.340.024.220.400', 'G05.360.340.024.340.137.515'], ['L01.453.245.667'], ['G02.111.570.820.486', 'G05.360.580'], ['G05.360.335.760.640', 'G05.360.340.024.340.137.650'], ['B01.046.550.550.600.700'], ['D13.444.735.500'], ['D13.444.735.686'], ['G02.111.570.080.708', 'G05.360.080.708'], ['E05.393.751'], ['E05.393.760.700']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Quality improvement and managed care as curriculum elements.
|
The authors describe a clinical course that incorporates practical experience in continuous quality improvement, case management, managed care, and healthcare systems analysis. Increasingly, nurses need to know and be able to use these concepts; therefore, they were added to the nursing curriculum. Although nursing students are able to articulate leadership and management theories, their ability to apply them is limited by lack of experience. This course is designed to provide a practical experience base that students may draw from upon entry into practice.
|
['Curriculum', 'Education, Nursing, Baccalaureate', 'Health Services Research', 'Humans', 'Managed Care Programs', 'Quality Assurance, Health Care']
| 8,336,854
|
[['I02.158'], ['I02.358.462.316'], ['H01.770.644.145.360', 'N03.349.380', 'N05.425'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['N03.219.521.576.343.800', 'N04.590.374.410'], ['N04.761.700', 'N05.700']]
|
['Anthropology, Education, Sociology, and Social Phenomena [I]', 'Disciplines and Occupations [H]', 'Health Care [N]', 'Organisms [B]']
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 1
| 0
|
Nrf2-mediated adaptive response to methyl glyoxal in HepG2 cells involves the induction of AKR7A2.
|
Methyl glyoxal (MG), a highly reactive dicarbonyl metabolite, causes a range of changes within the cell. It forms adducts with DNA and protein and contributes to the progression of several diseases as well as causing hepatic damage. In this study, we have used human hepatoma (HepG2) cells as a model to investigate the induction of protective enzymes in response to MG exposure. We have shown that treating HepG2 cells with sub-lethal concentrations of MG increases the level of NADPH:quinone oxidoreductase (NQO1) mRNA by 4.5-fold, AKR1C3 mRNA by 14-fold and AKR7A2 mRNA by 4-fold. Levels of AKR7A2 protein are increased by 2.1- and 1.8-fold following 9h and 24h exposure of cells to 50 ìM MG. The role of AKR7A2 in protecting HepG2 cells against MG toxicity was further investigated using specific siRNAs against AKR7A2 and Nrf2. Knockdown of AKR7A2 in HepG2 shows that AKR7A2 is responsible for up to 50% of the protection against MG toxicity in HepG2 cells. We have also shown that MG was able to induce the translocation of the transcription factor Nrf2 to the nucleus. HepG2 cells in which Nrf2 had been knocked down exhibited decreased NQO1 and AKR7A2 mRNA levels compared to control cells. In conclusion, these findings indicate that protective enzymes are significantly up-regulated in response to low concentrations of MG in HepG2 cells and that AKR7A2 contributes to protection against MG-induced toxicity. Nrf2 is critical in mediating MG induced expression of protective genes.
|
['3-Hydroxysteroid Dehydrogenases', 'Aldehyde Reductase', 'Aldo-Keto Reductase Family 1 Member C3', 'Cell Line, Tumor', 'Glyoxal', 'Hep G2 Cells', 'Humans', 'Hydroxyprostaglandin Dehydrogenases', 'NAD(P)H Dehydrogenase (Quinone)', 'NADP', 'NF-E2-Related Factor 2', 'RNA, Messenger', 'Up-Regulation']
| 25,451,587
|
[['D08.811.682.047.436.350'], ['D08.811.682.047.150.700.156.500', 'D08.811.682.047.820.284.500'], ['D08.811.682.047.150.700.156.875', 'D08.811.682.047.436.375.140', 'D08.811.682.047.820.284.937', 'D08.811.682.047.820.375.500'], ['A11.251.210.190', 'A11.251.860.180'], ['D02.047.644'], ['A11.251.860.180.432', 'A11.436.348.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D08.811.682.047.820.375'], ['D08.811.682.608.800.500'], ['D03.633.100.759.646.138.749', 'D08.211.625', 'D13.695.667.138.749', 'D13.695.827.068.749'], ['D12.776.260.108.737', 'D12.776.930.127.737'], ['D13.444.735.544'], ['G02.111.905', 'G05.308.850', 'G07.690.773.998']]
|
['Chemicals and Drugs [D]', 'Anatomy [A]', 'Organisms [B]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Patterns of VOC and BTEX concentration in ambient air around industrial sources in Daegu, Korea.
|
Patterns of VOC and BTEX (Benzene, Toluene, Ethylvenzene, and Xylene) distribution at industrial emission sources, proximal residential areas of industrial estates, and ambient air were studied in Daegu, Korea. Daytime and night-time sampling was done at 12 sites and 9 emission sources to provide samples for analyses, using the TO-14 method. Measured BTEX component ratios B/T, T/EB, T/X and EB/X in ambient air were found to be 2.6 g, 11.3 g, 1.0 g and 1.2 g in the residential area; 2.2 g, 11.0 g, 1.0 g and 1.6 g in the commercial area; and 1.0 g, 14.9 g, 1.0 g and 1.3 g in the industrial area. The significant difference observed between the ratios for the residential and commercial areas implies that the two areas have different emission sources. This is also indicated by the significant differences observed between daytime and nighttime BTEX concentrations. Toluene and xylene were detected at very high concentrations, at the sampling sites. This pattern reflects the type of industrial processes and materials that are managed at the emission sources, as well as topographic/climatic factors that impact upon pollutant transport processes in the atmosphere. The BTEX distribution pattern in Daegu is observed to be similar to that of several Asian cities, particularly Hong Kong. These results are useful in the design of emission source control measures for VOCs and BTEX in Daegu.
|
['Air Pollutants', 'Hydrocarbons, Aromatic', 'Industrial Waste', 'Korea', 'Volatile Organic Compounds']
| 19,085,600
|
[['D27.888.284.101'], ['D02.455.426.559'], ['D20.944.420', 'N06.850.460.710.420'], ['Z01.252.474.557', 'Z01.586.407'], ['D02.974']]
|
['Chemicals and Drugs [D]', 'Health Care [N]', 'Geographicals [Z]']
| 0
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
|
Microphthalmia with linear skin defects syndrome (MLS): a male with a mosaic paracentric inversion of Xp.
|
The microphthalmia with linear skin defects syndrome (MLS) is an X-linked dominant disorder with male lethality. In the majority of the patients reported, the MLS syndrome is caused by segmental monosomy of the Xp22.3 region. To date, five male patients with MLS and 46,XX karyotype ("XX males") have been described. Here we report on the first male case with MLS and an XY complement. The patient showed agenesis of the corpus callosum, histiocytoid cardiomyopathy, and lactic acidosis but no microphthalmia, and carried a mosaic subtle inversion of the short arm of the X chromosome in 15% of his peripheral blood lymphocytes, 46,Y,inv(X)(p22.13 approximately 22.2p22.32 approximately 22.33)[49]/46,XY[271]. By fluorescence IN SITU hybridization (FISH), we showed that YAC 225H10 spans the breakpoint in Xp22.3. End-sequencing and database analysis revealed a YAC insert of at least 416 kb containing the genes HCCS and AMELX, and exons 2-16 of ARHGAP6. Molecular cytogenetic data suggest that the Xp22.3 inversion breakpoint is located in intron 1 of ARHGAP6, the gene encoding the Rho GTPase activating protein 6. Future molecular studies in karyotypically normal female MLS patients to detect submicroscopic rearrangements including the ARHGAP6 gene as well as mutation screening of ARHGAP6 in patients with no obvious chromosomal rearrangements will clarify the role of this gene in MLS syndrome.
|
['Chromosome Inversion', 'Chromosomes, Human, X', 'Fatal Outcome', 'GTPase-Activating Proteins', 'Humans', 'In Situ Hybridization, Fluorescence', 'Infant', 'Karyotyping', 'Male', 'Microphthalmos', 'Mosaicism', 'Sex Chromosome Aberrations', 'Skin Abnormalities', 'Syndrome']
| 12,900,578
|
[['C23.550.210.190', 'G05.365.590.175.190', 'G05.365.590.770.500', 'G05.558.805.500'], ['A11.284.187.520.300.325.680', 'A11.284.187.865.982.500', 'G05.360.162.520.300.325.680', 'G05.360.162.865.982.500'], ['E05.318.308.985.550.325', 'N01.224.935.698.201', 'N06.850.505.400.975.550.325', 'N06.850.520.308.985.550.325'], ['D12.644.360.325.150', 'D12.776.476.325.150'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.225.500.620.670.325.350', 'E01.370.225.750.600.670.325.350', 'E05.200.500.620.670.325.350', 'E05.200.750.600.670.325.350', 'E05.393.285.350', 'E05.393.661.475.350'], ['M01.060.703'], ['E01.370.225.500.385.315', 'E05.200.500.385.315', 'E05.242.385.315', 'E05.393.285.475'], ['C11.250.566', 'C16.131.384.666'], ['G05.365.590.175.595'], ['C23.550.210.815', 'G05.365.590.175.815'], ['C16.131.831', 'C17.800.804'], ['C23.550.288.500']]
|
['Diseases [C]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Named Groups [M]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Impact of early intervention services on duration of untreated psychosis: data from the National EDEN prospective cohort study.
|
OBJECTIVE: This study aimed to determine if the inception of Early Intervention Services (EISs) is followed by an improvement in the prompt treatment of people with first episode psychosis.METHOD: A prospective cohort study of referrals to new and established EISs was conducted at 1, 2, 3, and 4 years after inception of new EIS. The study was conducted with 14 (seven new and seven established) secondary care EIS within geographically defined catchment areas in England between 2005 and 2009. Participants included 1027 consecutive referrals to EIS aged 14-35 with a first episode of psychosis. Duration of untreated psychosis (DUP) and number of participants treated adequately within 6 months of onset were the main outcome measures.RESULTS: A significant downward trend across yearly cohorts for DUP for new EIS (F1,549=8.4, p=0.004) but not for established EIS (F1,429=1.7, p=0.19) was observed. There was a significant upward trend across cohorts in the proportion of referrals treated within 6 months for new EIS (X(2)=8.0, df=1, p=0.005), but not for established EIS (X(2)=0.1, df=1, p=0.72).CONCLUSION: The introduction of new EIS was followed by a reduction in DUP and an increase in the proportion of patients treated within 6 months of onset. These trends were not present in the catchment areas of established services where DUP was initially lower, suggesting that there was no general tendency for DUP to fall over time. Hence, the introduction of an EIS was followed by an improvement in the prompt and proper treatment of first episode psychosis.
|
['Adolescent', 'Adult', 'Early Medical Intervention', 'England', 'Female', 'Humans', 'Longitudinal Studies', 'Male', 'Prospective Studies', 'Psychotic Disorders', 'Sensitivity and Specificity', 'Time-to-Treatment', 'Young Adult']
| 25,107,851
|
[['M01.060.057'], ['M01.060.116'], ['N02.421.726.363'], ['Z01.542.363.300'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.372.500.750.500', 'N05.715.360.330.500.750.500', 'N06.850.520.450.500.750.500'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['F03.700.675'], ['E05.318.370.800', 'E05.318.740.872', 'G17.800', 'N05.715.360.325.700', 'N05.715.360.750.725', 'N06.850.520.445.800', 'N06.850.520.830.872'], ['E02.760.928', 'N02.421.585.928'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Health Care [N]', 'Geographicals [Z]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Porous M(II)/pyrimidine-4,6-dicarboxylato neutral frameworks: synthetic influence on the adsorption capacity and evaluation of CO2-adsorbent interactions.
|
The understanding of the factors that affect the real pore-network structure for a given bulk material due to different synthetic procedures is essential to develop the material with the best adsorption properties. In this work, we have deeply studied the influence of the crystallinity degree over the adsorption capacity on three new isostructural MOFs with the formula {[CdM(ì4-pmdc)2(H2O)2]?solv}n (in which, pmdc = pyrimidine-4,6-dicarboxylate; solv = corresponding solvent; M(II) = Cd (1), Mn (2), Zn (3)). Compared with other methods, the solvent-free synthesis stands as the most effective route because, apart from enabling the preparation of the heterometallic compounds 2 and 3, it also renders the adsorbents with the highest performance, which is indeed close to the expected one derived from Grand Canonical Monte Carlo (GCMC) calculations. The structural analysis of the as-synthesised and evacuated frameworks reveals the existence of a metal atom exposed to the pore. The accessibility of this site is limited due to its atomic environment, which is why it is considered as a pseudo-open-metal site. The chemical and physical characterisation confirms that this site can be modified as the metal atom is replaced in compounds 2 and 3. To assess the effect of the metal replacement on the adsorption behaviour, an exhaustive study of CO2 experimental isotherms has been performed. The affinity of the pseudo-open metal sites towards CO2 and the distribution of the preferred adsorption sites are discussed on the basis of DFT and GCMC calculations.
|
['Adsorption', 'Carbon Dioxide', 'Carboxylic Acids', 'Metals', 'Models, Molecular', 'Organometallic Compounds', 'Porosity', 'Pyrimidines']
| 24,403,128
|
[['G01.030', 'G02.020'], ['D01.200.200', 'D01.362.150', 'D01.650.550.200'], ['D02.241'], ['D01.552'], ['E05.599.595'], ['D02.691'], ['G01.374.710'], ['D03.383.742']]
|
['Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Computational study on the reactions of H2O2 on TiO2 anatase (101) and rutile (110) surfaces.
|
This study investigates the adsorption and reactions of H(2)O(2) on TiO(2) anatase (101) and rutile (110) surfaces by first-principles calculations based on the density functional theory in conjunction with the projected augmented wave approach, using PW91, PBE, and revPBE functionals. Adsorption mechanisms of H(2)O(2) and its fragments on both surfaces are analyzed. It is found that H(2)O(2) , H(2)O, and HO preferentially adsorb at the Ti(5c) site, meanwhile HOO, O, and H preferentially adsorb at the (O(2c))(Ti(5c)), (Ti(5c))(2), and O(2c) sites, respectively. Potential energy profiles of the adsorption processes on both surfaces have been constructed using the nudged elastic band method. The two restructured surfaces, the 1/3 ML oxygen covered TiO(2) and the hydroxylated TiO(2), are produced with the H(2)O(2) dehydration and deoxidation, respectively. The formation of main products, H(2)O(g) and the 1/3 ML oxygen covered TiO(2) surface, is exothermic by 2.8 and 5.0 kcal/mol, requiring energy barriers of 0.8 and 1.1 kcal/mol on the rutile (110) and anatase (101) surface, respectively. The rate constants for the H(2)O(2) dehydration processes have been predicted to be 6.65 ? 10(-27) T(4.38) exp(-0.14 kcal mol(-1)/RT) and 3.18 ? 10(-23) T(5.60) exp(-2.92 kcal mol(-1)/RT) respectively, in units of cm(3) molecule(-1) s(-1).
|
['Adsorption', 'Hydrogen Peroxide', 'Quantum Theory', 'Surface Properties', 'Titanium']
| 21,387,334
|
[['G01.030', 'G02.020'], ['D01.248.497.158.685.750.424', 'D01.339.431.374.424', 'D01.650.550.750.400', 'D02.389.338.253'], ['H01.671.579.800'], ['G02.860'], ['D01.268.557.800', 'D01.268.956.878', 'D01.552.547.800']]
|
['Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Disciplines and Occupations [H]']
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
Gene cloning, heterologous overexpression and optimized refolding of the NAD-glutamate dehydrogenase from Haloferax mediterranei.
|
The NAD-dependent glutamate dehydrogenase (GDH) gene from the halophilic archaeon Haloferax mediterranei has been cloned. The analysis of the nucleotide sequence revealed an open reading frame of 1323 bp that encodes a NAD-GDH. The amino acid sequence displayed high homology with those from other sources, especially the highly conserved residues involved in 2-oxoglutarate binding. The expression of this gene in Escherichia coli, the refolding and further characterization, yielded a fully active NAD-GDH with the same features than those found for the wild-type enzyme. This halophilic NAD-GDH showed a highly dependence on salts for both stability and activity, being essential for the refolding of the recombinant enzyme.
|
['Amino Acid Sequence', 'Archaeal Proteins', 'Cloning, Molecular', 'Gene Library', 'Glutamate Dehydrogenase', 'Haloferax mediterranei', 'Hydrogen-Ion Concentration', 'Molecular Sequence Data', 'Polymerase Chain Reaction', 'Protein Folding', 'Recombinant Proteins', 'Sequence Homology, Amino Acid', 'Substrate Specificity', 'Temperature']
| 16,200,391
|
[['G02.111.570.060', 'L01.453.245.667.060'], ['D12.776.090'], ['E05.393.220'], ['G05.360.325'], ['D08.811.682.664.500.398'], ['B02.200.400.400.440.500'], ['G02.300'], ['L01.453.245.667'], ['E05.393.620.500'], ['G01.154.651', 'G02.111.688'], ['D12.776.828'], ['G02.111.810.200', 'G05.810.200'], ['G02.111.835'], ['G01.906.595', 'G16.500.275.063.725.710', 'G16.500.750.775.710', 'N06.230.150.450', 'N06.230.300.100.725.710']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Health Care [N]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 1
| 0
|
Synergistic effects of celecoxib and bupropion in a model of chronic inflammation-related depression in mice.
|
This study was aimed to characterize the depression-like behaviour in the classical model of chronic inflammation induced by Complete Freund's Adjuvant (CFA). Male Swiss mice received an intraplantar (i.pl.) injection of CFA (50 µl/paw) or vehicle. Behavioural and inflammatory responses were measured at different time-points (1 to 4 weeks), and different pharmacological tools were tested. The brain levels of IL-1â and BDNF, or COX-2 expression were also determined. CFA elicited a time-dependent edema formation and mechanical allodynia, which was accompanied by a significant increase in the immobility time in the tail suspension (TST) or forced-swimming (FST) depression tests. Repeated administration of the antidepressants imipramine (10 mg/kg), fluoxetine (20 mg/kg) and bupropion (30 mg/kg) significantly reversed depression-like behaviour induced by CFA. Predictably, the anti-inflammatory drugs dexamethasone (0.5 mg/kg), indomethacin (10 mg/kg) and celecoxib (30 mg/kg) markedly reduced CFA-induced edema. The oral treatment with the analgesic drugs dipyrone (30 and 300 mg/kg) or pregabalin (30 mg/kg) significantly reversed the mechanical allodyinia induced by CFA. Otherwise, either dipyrone or pregabalin (both 30 mg/kg) did not significantly affect the paw edema or the depressive-like behaviour induced by CFA, whereas the oral treatment with dipyrone (300 mg/kg) was able to reduce the immobility time in TST. Noteworthy, CFA-induced edema was reduced by bupropion (30 mg/kg), and depression behaviour was prevented by celecoxib (30 mg/kg). The co-treatment with bupropion and celecoxib (3 mg/kg each) significantly inhibited both inflammation and depression elicited by CFA. The same combined treatment reduced the brain levels of IL-1â, as well as COX-2 immunopositivity, whilst it failed to affect the reduction of BDNF levels. We provide novel evidence on the relationship between chronic inflammation and depression, suggesting that combination of antidepressant and anti-inflammatory agents bupropion and celecoxib might represent an attractive therapeutic strategy for depression.
|
['Analgesics', 'Animals', 'Anti-Inflammatory Agents', 'Antidepressive Agents', 'Behavior, Animal', 'Brain-Derived Neurotrophic Factor', 'Bupropion', 'Celecoxib', 'Cerebral Cortex', 'Cyclooxygenase 2', 'Depression', 'Dipyrone', 'Disease Models, Animal', 'Dose-Response Relationship, Drug', 'Drug Synergism', 'Edema', "Freund's Adjuvant", 'Inflammation', 'Interleukin-1beta', 'Male', 'Mice', 'Nociception', 'Pregabalin', 'Pyrazoles', 'Sulfonamides', 'gamma-Aminobutyric Acid']
| 24,086,771
|
[['D27.505.696.663.850.014', 'D27.505.954.427.040'], ['B01.050'], ['D27.505.954.158'], ['D27.505.954.427.700.122'], ['F01.145.113'], ['D12.644.276.860.100', 'D12.776.467.860.100', 'D12.776.631.600.100', 'D23.529.850.100'], ['D02.522.818.110'], ['D02.065.884.247', 'D02.886.590.700.247', 'D03.383.129.539.160'], ['A08.186.211.200.885.287.500'], ['D08.811.600.720.750'], ['F01.145.126.350'], ['D03.383.129.539.850.077.150'], ['C22.232', 'E05.598.500', 'E05.599.395.080'], ['G07.690.773.875', 'G07.690.936.500'], ['G07.690.773.968.477'], ['C23.888.277'], ['D20.475'], ['C23.550.470'], ['D12.644.276.374.465.010.600', 'D12.644.276.374.500.400.600', 'D12.776.467.374.465.010.600', 'D12.776.467.374.500.400.600', 'D23.529.374.465.131.600', 'D23.529.374.500.400.600'], ['B01.050.150.900.649.313.992.635.505.500'], ['F02.463.593.504.500'], ['D02.241.081.114.500.350.500', 'D12.125.190.350.450'], ['D03.383.129.539'], ['D02.065.884', 'D02.886.590.700'], ['D02.241.081.114.500.350', 'D12.125.190.350']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Psychiatry and Psychology [F]', 'Anatomy [A]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Preparation and characterisation of alendronate-loaded chitosan microparticles obtained through the spray drying technique.
|
Microparticles of chitosan (CHT) containing alendronate sodium (AL) were prepared in four drug:polymer ratios (1:1, 1:2, 1:4, 1:6) using the spray drying technique. The efficiency of the method was evaluated by determining production yield (about 70 %) and microencapsulation efficiency, which was almost 100 % in the case of all four of the formulations studied. Particles had a mean size of between 3.6 and 4.6 microm, and a near-spherical shape. The formulations with the highest content of AL (drug:polymer ratio 1:1 and 1:2) showed an asymmetrical distribution of particles, which were larger in size, and had a higher proportion of irregular particles than the other formulations. FT-IR analysis revealed an ionic interaction between AL and CHT. Differential scanning calorimetry and thermogravimetric analysis confirmed the microencapsulation of AL and the increased thermal stability of encapsulated AL. The dissolution profiles of AL from CHT microspheres, at pH values of 1.2 and 6.8, showed a delayed release of AL from microspheres, and the dissolution rate was dependent on the pH and the drug:polymer ratio. It can be concluded that spray drying is a suitable technique for preparing AL-loaded CHT microspheres, and that the drug:polymer ratio can be used to control the rate of AL release from microspheres.
|
['Alendronate', 'Biological Availability', 'Calorimetry, Differential Scanning', 'Chitosan', 'Drug Carriers', 'Humans', 'Hydrogen-Ion Concentration', 'Microscopy, Electron, Scanning', 'Microspheres', 'Particle Size', 'Spectrophotometry, Infrared', 'Surface Properties', 'Thermogravimetry']
| 19,275,718
|
[['D02.705.429.500.100'], ['G03.787.151', 'G07.690.725.129'], ['E05.196.131.310', 'E05.196.370.310'], ['D05.750.078.139.500', 'D09.698.211.500'], ['D26.255.260', 'E02.319.300.380'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G02.300'], ['E01.370.350.515.402.541', 'E05.595.402.541'], ['E07.565'], ['G02.712'], ['E05.196.712.726.676', 'E05.196.867.826.676'], ['G02.860'], ['E05.196.904']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Does the Janani Suraksha Yojana cash transfer programme to promote facility births in India ensure skilled birth attendance? A qualitative study of intrapartum care in Madhya Pradesh.
|
BACKGROUND: Access to facility delivery in India has significantly increased with the Janani Suraksha Yojana (JSY) cash transfer programme to promote facility births. However, a decline in maternal mortality has only followed secular trends as seen from the beginning of the decade well before the programme began. We, therefore, examined the quality of intrapartum care provided in facilities under the JSY programme to study whether it ensures skilled attendance at birth.DESIGN: 1) Non-participant observations (n=18) of intrapartum care during vaginal deliveries at a representative sample of 11 facilities in Madhya Pradesh to document what happens during intrapartum care. 2) Interviews (n=10) with providers to explore reasons for this care. Thematic framework analysis was used.RESULTS: Three themes emerged from the data: 1) delivery environment is chaotic: delivery rooms were not conducive to safe, women-friendly care provision, and coordination between providers was poor. 2) Staff do not provide skilled care routinely: this emerged from observations that monitoring was limited to assessment of cervical dilatation, lack of readiness to provide key elements of care, and the execution of harmful/unnecessary practices coupled with poor techniques. 3) Dominant staff, passive recipients: staff sometimes threatened, abused, or ignored women during delivery; women were passive and accepted dominance and disrespect. Attendants served as 'go-betweens' patients and providers. The interviews with providers revealed their awareness of the compromised quality of care, but they were constrained by structural problems. Positive practices were also observed, including companionship during childbirth and women mobilising in the early stages of labour.CONCLUSIONS: Our observational study did not suggest an adequate level of skilled birth attendance (SBA). The findings reveal insufficiencies in the health system and organisational structures to provide an 'enabling environment' for SBA. We highlight the need to ensure quality obstetric care prior to increasing coverage of facility births if cash transfer programmes like the JSY are to improve health outcomes.
|
['Adult', 'Attitude of Health Personnel', 'Environment', 'Female', 'Health Personnel', 'Health Services Accessibility', 'Humans', 'India', 'Interviews as Topic', 'Maternal Health Services', 'Maternal Mortality', 'Medical Assistance', 'Parturition', 'Patient Acceptance of Health Care', 'Program Evaluation', 'Qualitative Research', 'Quality of Health Care']
| 26,160,769
|
[['M01.060.116'], ['F01.100.050', 'N05.300.100'], ['G16.500.275', 'N06.230'], ['M01.526.485', 'N02.360'], ['N04.590.374.350', 'N05.300.430'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['Z01.252.245.393'], ['E05.318.308.420', 'L01.399.250.520', 'N05.715.360.300.400', 'N06.850.520.308.420'], ['N02.421.143.620', 'N02.421.800.500'], ['E05.318.308.985.550.500', 'N01.224.935.698.653', 'N06.850.505.400.975.550.500', 'N06.850.520.308.985.550.500'], ['N03.219.521.346.506.564'], ['G08.686.784.769.490'], ['F01.100.150.750.500', 'F01.145.488.887.500', 'N05.300.150.800.500'], ['E05.337.820', 'N04.761.685', 'N05.715.360.650'], ['H01.770.644.241.850'], ['N04.761', 'N05.715']]
|
['Named Groups [M]', 'Psychiatry and Psychology [F]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Information Science [L]', 'Disciplines and Occupations [H]']
| 0
| 1
| 0
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 1
| 1
| 1
|
Correlation between O6-methylguanine-DNA methyltransferase and survival in inoperable newly diagnosed glioblastoma patients treated with neoadjuvant temozolomide.
|
PURPOSE: This phase II study evaluated the efficacy and safety of a 7-day on/7-day off regimen of temozolomide before radiotherapy (RT) in patients with inoperable newly diagnosed glioblastoma.PATIENTS AND METHODS: Patients received temozolomide (150 mg/m2/d on days 1 to 7 and days 15 to 21 every 28 days; 7 days on/7 days off) for up to four cycles before conventional RT (2-Gy fractions to a total of 60 Gy) and for four cycles thereafter or until disease progression. The primary end point was tumor response. Tumor tissue from 25 patients was analyzed for O6-methylguanine-DNA methyltransferase (MGMT) expression.RESULTS: Twenty-nine patients with a median age of 60 years were treated, and 28 were assessable for response. Seven (24%) of 29 patients had a partial response, nine patients (31%) had stable disease, and 12 patients (41%) had progressive disease. Median progression-free survival (PFS) time was 3.8 months, and median overall survival (OS) time was 6.1 months. Patients with low MGMT expression, compared with patients with high MGMT expression, had a significantly higher response rate (55% v 7%, respectively; P = .004) and improved PFS (median, 5.5 v 1.9 months, respectively; P = .009) and OS (median, 16 v 5 months, respectively; P = .003). The most common grade 3 and 4 toxicities were thrombocytopenia (20%) and neutropenia (17%).CONCLUSION: This dose-dense temozolomide regimen resulted in modest antitumor activity with an acceptable safety profile in the neoadjuvant setting, and expression of MGMT correlated with response to temozolomide. However, this treatment approach seems to be inferior to standard concomitant RT plus temozolomide.
|
['Adolescent', 'Adult', 'Aged', 'Biomarkers, Tumor', 'Brain Neoplasms', 'Chemotherapy, Adjuvant', 'Dacarbazine', 'Dose-Response Relationship, Drug', 'Drug Administration Schedule', 'Follow-Up Studies', 'Glioblastoma', 'Humans', 'Magnetic Resonance Imaging', 'Maximum Tolerated Dose', 'Middle Aged', 'Neoplasm Staging', 'O(6)-Methylguanine-DNA Methyltransferase', 'Probability', 'Prospective Studies', 'Risk Assessment', 'Survival Analysis', 'Temozolomide', 'Treatment Outcome']
| 17,442,989
|
[['M01.060.057'], ['M01.060.116'], ['M01.060.116.100'], ['D23.101.140'], ['C04.588.614.250.195', 'C10.228.140.211', 'C10.551.240.250'], ['E02.186.170', 'E02.319.170'], ['D02.925.200', 'D03.383.129.308.240'], ['G07.690.773.875', 'G07.690.936.500'], ['E02.319.283'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['C04.557.465.625.600.380.080.335', 'C04.557.470.670.380.080.335', 'C04.557.580.625.600.380.080.335'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.350.825.500'], ['E05.940.481', 'G07.690.936.625'], ['M01.060.116.630'], ['E01.789.625'], ['D08.811.913.555.500.800.650'], ['E05.318.740.600', 'G17.680', 'N05.715.360.750.625', 'N06.850.520.830.600'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['E05.318.740.600.800.715', 'N04.452.871.715', 'N05.715.360.750.625.700.690', 'N06.850.505.715', 'N06.850.520.830.600.800.715'], ['E05.318.740.998', 'N05.715.360.750.795', 'N06.850.520.830.998'], ['D02.925.200.500', 'D03.383.129.308.240.500'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Food irradiation: the process and implications for dietitians.
|
Despite the limited use of irradiation for food preservation in the United States to date, the process provides an alternative to the use of some chemical pesticides and sprout inhibitors. The formation of random and varied radiolytic products (RPs) in foods that have been irradiated is the focus of criticism of the process, because RPs may affect the sensory and nutritive quality of foods processed with ionizing radiation. The FDA has deemed the process safe, within specified doses, for use on spices, some meats, fruits, and vegetables. Dietitians should be prepared to answer consumer questions related to irradiation as the process becomes more widespread.
|
['Chemical Phenomena', 'Chemistry', 'Dietary Carbohydrates', 'Dietary Fats', 'Dietary Proteins', 'Dietetics', 'Food Irradiation', 'Vitamins']
| 3,385,107
|
[['G02'], ['H01.181'], ['D09.301', 'G07.203.300.362', 'J02.500.362'], ['D10.212.302', 'G07.203.300.375', 'J02.500.375'], ['D12.776.256', 'G07.203.300.428', 'J02.500.428'], ['H02.533.290'], ['J01.576.423.850.700.700.500'], ['D27.505.696.494.600', 'G07.203.300.681.500.600', 'J02.500.681.500.600']]
|
['Phenomena and Processes [G]', 'Disciplines and Occupations [H]', 'Chemicals and Drugs [D]', 'Technology, Industry, and Agriculture [J]']
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
|
Conformational restrictions in ligand binding to the human intestinal di-/tripeptide transporter: implications for design of hPEPT1 targeted prodrugs.
|
The aim of the present study was to develop a computational method aiding the design of dipeptidomimetic pro-moieties targeting the human intestinal di-/tripeptide transporter hPEPT1. First, the conformation in which substrates bind to hPEPT1 (the bioactive conformation) was identified by conformational analysis and 2D dihedral driving analysis of 15 hPEPT1 substrates, which suggested that psi(1) approximately 165 degrees , omega(1) approximately 180 degrees , and phi(2) approximately 280 degrees were descriptive of the bioactive conformation. Subsequently, the conformational energy required to change the peptide backbone conformation (DeltaE(bbone)) from the global energy minimum conformation to the identified bioactive conformation was calculated for 20 hPEPT1 targeted model prodrugs with known K(i) values. Quantitatively, an inverse linear relationship (r(2)=0.81, q(2)=0.80) was obtained between DeltaE(bbone) and log1/K(i), showing that DeltaE(bbone) contributes significantly to the experimentally observed affinity for hPEPT1 ligands. Qualitatively, the results revealed that compounds classified as high affinity ligands (K(i)<0.5 mM) all have a calculated DeltaE(bbone)<1 kcal/mol, whereas medium and low-affinity compounds (0.5 mM<K(i)<15 mM) have DeltaE(bbone) values in the range 1-3 kcal/mol. The findings also shed new light on the basis for the experimentally observed stereoselectivity of hPEPT1.
|
['Amination', 'Dipeptides', 'Drug Design', 'Humans', 'Intestines', 'Ligands', 'Molecular Structure', 'Peptide Transporter 1', 'Prodrugs', 'Symporters', 'Water', 'beta-Lactams']
| 15,727,852
|
[['G02.111.053', 'G02.607.110', 'G03.068'], ['D12.644.456.345'], ['E05.290.500', 'H01.158.703.007.338.500', 'H01.181.466.338.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A03.556.124'], ['D27.720.470.480'], ['G02.111.570', 'G02.466'], ['D12.776.157.530.450.625.202', 'D12.776.157.530.937.613', 'D12.776.543.585.450.625.202', 'D12.776.543.585.937.702'], ['D26.675'], ['D12.776.157.530.450.625', 'D12.776.543.585.450.625'], ['D01.045.250.875', 'D01.248.497.158.459.650', 'D01.650.550.925'], ['D02.065.589.099', 'D02.886.108', 'D03.633.100.300']]
|
['Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Organisms [B]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
Normalize the response of EPID in pursuit of linear accelerator dosimetry standardization.
|
Normalize the response of electronic portal imaging device (EPID) is the first step toward an EPID-based standardization of Linear Accelerator (linac) dosimetry quality assurance. In this study, we described an approach to generate two-dimensional (2D) pixel sensitivity maps (PSM) for EPIDs response normalization utilizing an alternative beam and dark-field (ABDF) image acquisition technique and large overlapping field irradiations. The automated image acquisition was performed by XML-controlled machine operation and the PSM was generated based on a recursive calculation algorithm for Varian linacs equipped with aS1000 and aS1200 imager panels. Cross-comparisons of normalized beam profiles and 1.5%/1.5 mm 1D Gamma analysis was adopted to quantify the improvement of beam profile matching before and after PSM corrections. PSMs were derived for both photon (6, 10, 15 MV) and electron (6, 20 MeV) beams via proposed method. The PSM-corrected images reproduced a horn-shaped profile for photon beams and a relative uniform profiles for electrons. For dosimetrically matched linacs equipped with aS1000 panels, PSM-corrected images showed increased 1D-Gamma passing rates for all energies, with an average 10.5% improvement for crossline and 37% for inline beam profiles. Similar improvements in the phantom study were observed with a maximum improvement of 32% for 15 MV and 22% for 20 MeV. The PSM value showed no significant change for all energies over a 3-month period. In conclusion, the proposed approach correct EPID response for both aS1000 and aS1200 panels. This strategy enables the possibility to standardize linac dosimetry QA and to benchmark linac performance utilizing EPID as the common detector.
|
['Algorithms', 'Electrical Equipment and Supplies', 'Humans', 'Particle Accelerators', 'Phantoms, Imaging', 'Photons', 'Quality Assurance, Health Care', 'Quality Control', 'Radiotherapy Dosage', 'Radiotherapy Planning, Computer-Assisted', 'Radiotherapy, Intensity-Modulated']
| 29,125,224
|
[['G17.035', 'L01.224.050'], ['E07.305'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E07.710.680'], ['E07.671'], ['G01.249.705', 'G01.358.500.505.650.782', 'G01.590.540.782', 'G01.750.250.650.782', 'G01.750.770.578.782'], ['N04.761.700', 'N05.700'], ['J01.897.608'], ['E02.815.639'], ['E02.950.825', 'L01.313.500.750.100.710.600.608'], ['E02.815.635.700.700', 'L01.313.500.750.100.710.600.550.700']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Health Care [N]', 'Technology, Industry, and Agriculture [J]']
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 1
| 0
|
A 2-year follow-up of root coverage using sub-pedicle acellular dermal matrix allografts and subepithelial connective tissue autografts.
|
BACKGROUND: Coverage of roots exposed by gingival recession is one of the main objectives of periodontal reconstructive surgery. A large variety of mucogingival grafting procedures are available. However, the long-term effectiveness of this procedure is still not clear. This study compared the effectiveness of sub-pedicle acellular dermal matrix allografts with subepithelial connective tissue autografts in achieving root coverage 2 years postoperatively.METHODS: One hundred one (101) patients were treated with dermal matrix allografts (mean age, 28.4+/- 0.7 years; mean recession, 4.2 mm) and 65 patients treated with connective tissue graft (mean age, 30.1+/- 1.4 years; mean recession, 4.9 mm). All patients underwent full periodontal evaluation and presurgical preparation, including oral hygiene instruction and scaling and root planing. The exposed roots were thoroughly planed and covered by a graft without any further root treatment or conditioning. There were no differences in the average age, time of follow-up, or gender between the two groups. Patients were evaluated periodically between 1 and 2 years. Residual recession and defect coverage were assessed.RESULTS: Mean residual root recession after root coverage with acellular dermal matrix allograft was 0.2 +/- 0.04 mm, with defect coverage of 95.9% +/- 0.9%. Frequency of defect coverage was 82.2%. Root coverage was 98.8% +/- 0.2%, resulting in a frequency of root coverage of 100%. Gain in keratinized gingiva was 2.2+/- 0.04 mm and attachment gain was 4.5+/- 0.1 mm per patient. Connective tissue autografts resulted in mean residual root recession of 0.1+/- 0.04 mm, with percent defect coverage of 97.8%+/- 0.6% and frequency of defect coverage of 95.4%. Root coverage was 99.1%+/- 0.2%, and frequency of root coverage was 100%. Gain in keratinized gingiva was 3.0+/- 0.1 mm and attachment gain was 5.3+/- 0.2 mm per patient. No significant differences in final recession and root coverage between the two treatment methods were found. However, autografts resulted in significant increases in defect coverage, keratinized gingival gain, attachment gain, and residual probing depth. The clinical results were stable for the 2-year follow-up period.CONCLUSIONS: These results indicate that coverage of root by sub-pedicle acellular dermal matrix allografts or subepithelial connective tissue autografts is a very predictable procedure which is stable for 2 years postoperatively. However, subepithelial connective tissue autografts resulted in significant increases in defect coverage, keratinized gingival gain, attachment gain, and residual probing depth.
|
['Adult', 'Connective Tissue', 'Female', 'Follow-Up Studies', 'Gingival Recession', 'Humans', 'Male', 'Mouth Mucosa', 'Retrospective Studies', 'Skin Transplantation', 'Skin, Artificial']
| 16,101,365
|
[['M01.060.116'], ['A10.165'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['C07.465.714.258.447', 'C07.465.714.354.625'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A10.615.550.599', 'A14.549.512'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E02.095.147.725.700', 'E04.680.275.850', 'E04.936.580.700'], ['E07.858.082.800']]
|
['Named Groups [M]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Diseases [C]', 'Organisms [B]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Synthesis and anxiolytic activity of N-substituted cyclic imides (1R*,2S*,3R*,4S*)-N-[4-[4-(2-pyrimidinyl)-1-piperazinyl]butyl]-2,3- bicyclo[2.2.1]heptanedicarboximide (tandospirone) and related compounds.
|
A series of cyclic imides bearing a omega-(4-aryl and 4-heteroaryl-1-piperazinyl)alkyl moieties was synthesized and tested in vivo for anxiolytic activity. The in vitro binding affinities of these compounds were also examined for 5-HT1A receptor sites. Structure-activity relationships within these series are discussed. One of these compounds, (1R*,2S*,-3R*,4S*)-N-[4-[4-(2-pyrimidinyl)-1-piperazinyl]butyl]-2,3- bicyclo[2.2.1]heptanedicarboximide (1: tandospirone), was found to be equipotent with buspirone in its anxiolytic activity and more anxio-selective than buspirone and diazepam. Tandospirone (1) is currently undergoing clinical evaluation as a selective anxiolytic agent.
|
['Animals', 'Anti-Anxiety Agents', 'Imides', 'Isoindoles', 'Piperazines', 'Pyrimidines', 'Rats']
| 1,687,114
|
[['B01.050'], ['D27.505.696.277.950.015', 'D27.505.954.427.210.950.015', 'D27.505.954.427.700.872.015'], ['D02.478'], ['D03.633.100.513'], ['D03.383.606'], ['D03.383.742'], ['B01.050.150.900.649.313.992.635.505.700']]
|
['Organisms [B]', 'Chemicals and Drugs [D]']
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Prevalence of cutaneous findings in Spanish neonates and relationships to obstetric and parental factors.
|
Two hundred forty-seven healthy newborns were investigated in a prospective cohort descriptive study. Information on phenotype and obstetric and parental history was collected. A positive association was found between erythema toxicum neonatorum and season of birth (spring and summer), whereas parental history of any skin disease was related to a lower frequency of this eruption.
|
['Adult', 'Delivery, Obstetric', 'Dermatitis, Atopic', 'Female', 'Humans', 'Infant, Newborn', 'Infant, Newborn, Diseases', 'Male', 'Prevalence', 'Prospective Studies', 'Seasons', 'Skin Diseases', 'Spain']
| 21,995,510
|
[['M01.060.116'], ['E04.520.252'], ['C16.320.850.210', 'C17.800.174.193', 'C17.800.815.193', 'C17.800.827.210', 'C20.543.480.343'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703.520'], ['C16.614'], ['E05.318.308.985.525.750', 'N01.224.935.597.750', 'N06.850.505.400.975.525.750', 'N06.850.520.308.985.525.750'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['G01.910.645.661', 'G16.500.275.071.590', 'N06.230.300.100.250.525'], ['C17.800'], ['Z01.542.846']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Organisms [B]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Geographicals [Z]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Evolution of low-copy number and major satellite DNA sequences coexisting in two Pimelia species-groups (Coleoptera).
|
Satellite DNA sequence evolution has been studied in several insect species from the genus Pimelia (Tenebrionidae, Coleoptera). Low-copy number homologs of the previously characterized major satellite DNA from P. monticola (PMON) have been cloned and sequenced from six congeneric species belonging to two species groups: Ibero-Balearic and Moroccan. Sequence analysis of a sample of low-copy number repeats revealed two subfamilies, differing on average 17.5% due to randomly spread single point mutations. Each subfamily is specific for a group of taxa in congruence with their biogeography. Within each group, there is no significant species-specific clustering of the sequences. These results suggest that the two satellite subfamilies arose after the split of an ancestral lineage into the North African and Ibero-Balearic Pimelia species-groups, but before their subsequent radiation. Rate heterogeneity tests suggest that PMON sequences have evolved faster in the lineage leading to the Moroccan group. Comparison of sequence divergences between minor PMON and the previously characterized major PIM357 satellite obtained from the same taxa, points to similar evolutionary dynamics. Both sequences are evolving in parallel accumulating mutations in a gradual manner irrespectively of significant differences in abundance. These data show that copy number of the sequence families does not necessarily affect the sequence change dynamics of satellite repeats.
|
['Animals', 'Base Sequence', 'Coleoptera', 'DNA', 'DNA, Satellite', 'Evolution, Molecular', 'Gene Dosage', 'Genetic Variation', 'Molecular Sequence Data', 'Phylogeny', 'Repetitive Sequences, Nucleic Acid', 'Sequence Alignment', 'Sequence Analysis, DNA', 'Sequence Homology, Nucleic Acid', 'Species Specificity']
| 12,909,343
|
[['B01.050'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['B01.050.500.131.617.720.500.500.375'], ['D13.444.308'], ['D13.444.308.480', 'G02.111.570.080.708.800.150', 'G05.360.080.708.800.150', 'G05.360.340.024.220.150', 'G05.360.340.024.850.150'], ['G05.045.250', 'G16.075.250'], ['G05.380.350'], ['G05.365'], ['L01.453.245.667'], ['G05.697', 'G16.075.605', 'L01.100.697'], ['G02.111.570.080.708', 'G05.360.080.708'], ['E05.393.751'], ['E05.393.760.700'], ['G02.111.810.550', 'G05.810.550'], ['G16.824']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Reduction in potassium concentration of stored red blood cell units using a resin filter.
|
BACKGROUND: Hyperkalemia is a serious complication of rapid and massive blood transfusion due to high plasma potassium (K) in stored red blood cell (RBC) units. A potassium adsorption filter (PAF) was developed in Japan to remove K by exchanging with sodium (Na). We performed an in vitro evaluation of its efficacy and feasibility of use.STUDY DESIGN AND METHODS: Three AS-3 RBC units were filtered by each PAF using gravity; 10 PAFs were tested. Blood group, age, flow rate, and irradiation status were recorded. Total volume, K, Na, Cl, Mg, total Ca (tCa), RBC count, hemoglobin (Hb), hematocrit (Hct), and plasma Hb were measured before and after filtering each unit. Ionized Ca (iCa), pH, and glucose were measured for some units.RESULTS: After filtration, the mean decrease in K was 97.5% in the first RBC unit, 91.2% in the second unit, and 64.4% in the third unit. The mean increases in Na, Mg, and tCa were 33.0, 151.4, and 116.1%, respectively. iCa and pH remained low; glucose was unchanged. RBC count, Hb, and Hct decreased slightly after filtration of first units; plasma Hb was unchanged. After filtration, there was no visual evidence of increased hemolysis or clot formation.CONCLUSION: The PAF decreased K concentration in stored AS-3 RBC units to minimal levels in the first and second RBC units. Optimally, one filter could be used for 2 RBC units. Although Na increased, the level may not be clinically significant. PAF may be useful for at-risk patients receiving older units or blood that has been stored after gamma irradiation.
|
['Erythrocytes', 'Filtration', 'Humans', 'Potassium']
| 20,561,298
|
[['A11.118.290', 'A11.443.240', 'A15.145.229.334'], ['E05.196.454', 'G01.280', 'G02.263'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D01.268.549.550', 'D01.268.557.575', 'D01.552.528.652', 'D01.552.547.650']]
|
['Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Chemicals and Drugs [D]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[Our experience with the diagnosis and treatment of biliary pancreatitis].
|
The diagnostic and therapeutic problems faced during treatment of thirty-nine patients presenting biliopancreatitis (BP) are discussed. BP diagnosis is made on the ground of clinical picture, laboratory indicators, abdominal ultrasonography, intraoperative cholangio-pancreatography and cholangioscopy. Patients with cholelithiasis (ChL) of long-standing in the previous history are predominant, with the age group exceeding 50 years of age being most numerous, and the female gender prevailing. All patients undergo conservative pre- and postoperative treatment. Operative treatment in BP is performed as an emergency intervention. Cholecystectomy is done in all cases, and in 56.3 per cent of them it proves sufficient to promote a favourable outcome of the pathologic condition. External or internal drainage of the choledochus is necessitated in the presence of definite indications (obstruction of extrahepatic biliary ducts and pancreas documented by operative cholangiography, choledochoscopy and probing). Dilatation of the papillary sphincter is carried out in five patients (12.8 per cent) because of partial papillary stenosis. The destructive forms of pancreatitis are treated by gland draining in conjunction with necrectomy and drainage of the extrahepatic biliary ducts. A correlation is established between the incidence of destructive forms of pancreatitis and therapeutic results, on the one hand, and timing of the operative intervention, on the other. Operative management of BP is a method of choice insofar as it contributes to the complex and thorough treatment of the condition. Preoperative BP diagnosis is still a problem not well enough clarified which leads to delayed operation with an adverse impact on the prognosis of the disease.
|
['Acute Disease', 'Adult', 'Cholecystectomy', 'Cholelithiasis', 'Drainage', 'Female', 'Humans', 'Male', 'Middle Aged', 'Necrosis', 'Pancreas', 'Pancreatectomy', 'Pancreatitis']
| 7,877,262
|
[['C23.550.291.125'], ['M01.060.116'], ['E04.210.120.172'], ['C06.130.409'], ['E02.309', 'E04.237'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['C23.550.717'], ['A03.734'], ['E04.210.752'], ['C06.689.750']]
|
['Diseases [C]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Anatomy [A]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
[The results of using Virolex (acyclovir) in patients with herpetic keratitis].
|
Virolex (acyclovir) used as 3% ointment in 50 patients (50 eyes) with herpetic keratitis was found highly effective in dendritic keratitis and sufficiently effective in keratoiridocyclitis with ulcerations (megaherpetic keratitis), providing cure in 92 and 75%, of cases, respectively. The drug is ineffective in the treatment of stromal herpetic keratitis not associated with corneal ulcers. 3% virolex ointment application in superficial forms of herpetic keratitis is more effective than instillations of 0.1% keracide.
|
['Acyclovir', 'Adolescent', 'Adult', 'Aged', 'Anti-Infective Agents, Local', 'Drug Evaluation', 'Drug Therapy, Combination', 'Female', 'Humans', 'Idoxuridine', 'Keratitis, Herpetic', 'Male', 'Middle Aged', 'Mydriatics', 'Ointments']
| 8,310,597
|
[['D03.633.100.759.758.399.454.250'], ['M01.060.057'], ['M01.060.116'], ['M01.060.116.100'], ['D27.505.954.122.187'], ['E05.290.625', 'E05.337.425'], ['E02.319.310'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D03.383.742.680.852.300.400', 'D13.570.230.430.609', 'D13.570.685.852.300.400'], ['C01.375.725.465', 'C01.925.256.466.382.465', 'C01.925.325.465', 'C11.204.564.425', 'C11.294.800.475'], ['M01.060.116.630'], ['D27.505.696.663.050.500'], ['D26.255.640']]
|
['Chemicals and Drugs [D]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
[APPLICATION OF FREE BILOBED MEDIAL SURAL ARTERY PERFORATOR FLAP FOR REPAIRING PENETRATING WOUND OF FOOT].
|
OBJECTIVE: To explore the effectiveness of the free bilobed medial sural artery perforator (BMSAP) flap to repair penetrating wound of the foot.METHODS: Between April 2012 and October 2014, 7 cases of foot penetrating wounds were treated with the BMSAP flap. There were 5 males and 2 females, aged from 21 to 43 years (mean, 31.5 years). The causes of injury included the crush injury (4 cases), blunt puncture (2 cases), and firearm injury (1 case). The wound was located at the left foot in 4 cases and at the right foot in 3 cases. There were longitudinal penetrating injury in 5 cases and transverse penetrating injury in 2 cases. The size of wound ranged from 4 cm x 3 cm to 9 cm x 7 cm. The interval between injury and admission was 0.5-5.5 hours (mean, 3.2 hours). The free BMSAP flap of 5 cm x 4 cm-10 cm x 8 cm in size was used to repair the wounds on both sides and to reconstruct the sensation. The donor site was sutured or repaired with skin graft.RESULTS: After operation, 1 case had distal flap necrosis, the flap survived after dressing change; 1 case had wound infection, and delayed healing was obtained after drainage; and the flap survived completely, and primary healing was obtained in the other 5 cases. The skin grafts survived and healing of incision by first intention was observed at donor sites. The patients were followed up from 7 to 24 months (mean, 12.5 months). The flap had soft texture and similar color to normal skin. According to the British Medical Research Council (BMRC) sensory function assessment system, 1 case was rated as S2, 4 cases as , and 2 cases as S₃+. The American Orthopaedic Foot and Ankle Society (AOFAS) score was 86-97 (mean, 93.6); the results were excellent in 6 cases and good in 1 case.CONCLUSION: The free BMSAP flap is very suitable to repair penetrating wound of the foot. The flap has the advantages of repairing the two wounds at the same time and reconstructing skin sensation as well.
|
['Adult', 'Drainage', 'Female', 'Femoral Artery', 'Foot', 'Humans', 'Male', 'Necrosis', 'Perforator Flap', 'Reconstructive Surgical Procedures', 'Sensation', 'Skin', 'Skin Transplantation', 'Soft Tissue Injuries', 'Sural Nerve', 'Treatment Outcome', 'Wound Healing', 'Wounds, Penetrating']
| 27,044,222
|
[['M01.060.116'], ['E02.309', 'E04.237'], ['A07.015.114.351'], ['A01.378.610.250'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C23.550.717'], ['A10.850.710.750', 'E07.862.710.750'], ['E04.680'], ['F02.830.816', 'G11.561.790'], ['A17.815'], ['E02.095.147.725.700', 'E04.680.275.850', 'E04.936.580.700'], ['C26.808'], ['A08.800.800.720.450.760.820.820'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['G16.762.891'], ['C26.986']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Organisms [B]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]', 'Health Care [N]']
| 1
| 1
| 1
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Inhibition of survivin reduces HIF-1á, TGF-â1 and TFE3 in salivary adenoid cystic carcinoma.
|
In the present study, we explored the expression and correlation of survivin with HIF-1á, TGF-â1 and TFE3 in adenoid cystic carcinoma (AdCC). The expression of survivin, HIF-1á, TGF-â1 and TFE3 was assessed by immunohistochemical staining of a tissue microarray containing tissue samples of normal salivary gland (NSG), pleomorphic adenoma (PA) and AdCC. Correlation analysis of these proteins revealed that increased survivin expression was associated with the overexpression of HIF-1á (P<0.001, r = 0.5599), TGF-â1 (P<0.001, r = 0.6616) and TFE3 (P<0.001, r = 0.7747). The expression of survivin, HIF-1á, TGF-â1 and TFE3 was not correlated with the pathological type of human AdCC (P>0.05). Selective inhibition of survivin by YM155 and siRNA significantly reduced human SACC-83 cell proliferation, with the corresponding decrease in expression of HIF-1á, TGF-â1 and TFE3. The data indicate that the overexpression of survivin in AdCC is related to HIF-1á, TGF-â1 and TFE3. We hypothesize from these findings that the inhibition of survivin may be a novel strategy for neoadjuvant chemotherapeutic and radiosensitive treatment of AdCC.
|
['Basic Helix-Loop-Helix Leucine Zipper Transcription Factors', 'Carcinoma, Adenoid Cystic', 'Case-Control Studies', 'Cell Line, Tumor', 'Cluster Analysis', 'Gene Expression', 'Gene Expression Regulation, Neoplastic', 'Humans', 'Hypoxia-Inducible Factor 1, alpha Subunit', 'Imidazoles', 'Immunohistochemistry', 'Inhibitor of Apoptosis Proteins', 'Naphthoquinones', 'Neoplasm Grading', 'Salivary Gland Neoplasms', 'Survivin', 'Transforming Growth Factor beta1']
| 25,485,635
|
[['D12.776.260.103.500', 'D12.776.260.108.092', 'D12.776.930.125.500', 'D12.776.930.127.092'], ['C04.557.470.200.025.220'], ['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['A11.251.210.190', 'A11.251.860.180'], ['E05.318.740.250', 'N05.715.360.750.200', 'N06.850.520.830.250'], ['G05.297'], ['G05.308.370'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.776.260.103.625.750', 'D12.776.930.125.625.750'], ['D03.383.129.308'], ['E01.370.225.500.607.512', 'E01.370.225.750.551.512', 'E05.200.500.607.512', 'E05.200.750.551.512', 'E05.478.583', 'H01.158.100.656.234.512', 'H01.158.201.344.512', 'H01.158.201.486.512', 'H01.181.122.573.512', 'H01.181.122.605.512'], ['D08.811.464.938.750.210', 'D12.644.360.075.437', 'D12.776.476.075.437'], ['D02.455.426.559.847.638.721', 'D02.806.550', 'D04.615.638.721'], ['E01.789.612'], ['C04.588.443.591.824', 'C07.465.530.824', 'C07.465.815.718'], ['D12.644.360.075.437.625', 'D12.776.167.576', 'D12.776.220.600.450.495', 'D12.776.476.075.437.625'], ['D12.644.276.374.687.100', 'D12.644.276.954.775.100', 'D12.776.467.374.687.100', 'D12.776.467.942.775.100', 'D23.529.374.687.100', 'D23.529.942.775.100']]
|
['Chemicals and Drugs [D]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Disciplines and Occupations [H]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
|
Physico-chemical and Histomorphometric Evaluation of Zinc-containing Hydroxyapatite in Rabbits Calvaria.
|
The aim of this study was to characterize the physico-chemical properties and bone repair after implantation of zinc-containing nanostructured porous hydroxyapatite scaffold (nZnHA) in rabbits' calvaria. nZnHA powder containing 2% wt/wt zinc and stoichiometric nanostructured porous hydroxyapatite (nHA - control group) were shaped into disc (8 mm) and calcined at 550 °C. Two surgical defects were created in the calvaria of six rabbits (nZnHA and nHA). After 12 weeks, the animals were euthanized and the grafted area was removed, fixed in 10% formalin with 0.1 M phosphate buffered saline and embedded in paraffin (n=10) for histomorphometric evaluation. In addition, one sample from each group (n=2) was embedded in methylmethacrylate for the SEM and EDS analyses. The thermal treatment transformed the nZnHA disc into a biphasic implant composed of Zn-containing HA and Zn-containing â-tricalcium phosphate (ZnHA/âZnTCP). The XRD patterns for the nHA disc were highly crystalline compared to the ZnHA disc. Histological analysis revealed that both materials were biologically compatible and promoted osteoconduction. X-ray fluorescence and MEV-EDS of nZnHA confirmed zinc in the samples. Histomorphometric evaluation revealed the presence of new bone formation in both frameworks but without statistically significant differences (p>0.05), based on the Wilcoxon test. The current study confirmed that both biomaterials improve bone repair, are biocompatible and osteoconductive, and that zinc (2wt%) did not increase the bone repair. Additional in vivo studies are required to investigate the effect of doping hydroxyapatite with a higher Zn concentration.
|
['Animals', 'Biocompatible Materials', 'Durapatite', 'Fluorescence', 'Microscopy, Electron, Scanning', 'Rabbits', 'Skull', 'Spectrophotometry, Infrared', 'X-Ray Diffraction', 'Zinc']
| 27,982,185
|
[['B01.050'], ['D25.130', 'D27.720.102.130', 'J01.637.051.130'], ['D01.029.260.700.675.374.075.025.300.150', 'D01.146.360.050.300.200', 'D01.578.122.477.300', 'D01.695.625.675.650.075.025.300.150'], ['G01.358.500.505.650.665.500', 'G01.590.540.665.500'], ['E01.370.350.515.402.541', 'E05.595.402.541'], ['B01.050.150.900.649.313.968.700'], ['A02.835.232.781'], ['E05.196.712.726.676', 'E05.196.867.826.676'], ['E05.196.309.742', 'E05.196.822.950', 'G01.867.950', 'G02.965'], ['D01.268.556.940', 'D01.268.956.906', 'D01.552.544.940']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Technology, Industry, and Agriculture [J]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Pulmonary tumor microembolism.
|
Pulmonary tumor embolism is an often missed antemortem diagnosis in patients with cancer and respiratory failure. Although rare, this complication is an important cause of additional morbidity. Referred for radionuclide pulmonary perfusion and ventilation scintigraphy, a typical pattern of multiple subsegmental peripheral defects on perfusion lung scanning without matching ventilation defects, suggesting a high probability of pulmonary thromboembolism, often leads to false conclusions. We present a case of bilateral multiple subsegmental mismatched defects in lung ventilation perfusion scintigraphy, where autopsy confirmed the diagnosis of pulmonary tumor embolism, secondary to an undifferentiated ductal type adenocarcinoma of the pancreas. Pulmonary tumor embolism is an entity to keep in mind in patients treated for carcinoma presenting with (sub) acute dyspnea.
|
['Adenocarcinoma', 'Autopsy', 'Diagnosis, Differential', 'Dyspnea', 'Fatal Outcome', 'Humans', 'Lung Neoplasms', 'Male', 'Middle Aged', 'Pancreatic Neoplasms', 'Pulmonary Embolism', 'Radiography, Thoracic', 'Radionuclide Imaging']
| 9,650,217
|
[['C04.557.470.200.025'], ['E01.370.060', 'E05.070', 'I01.198.780.937.120'], ['E01.171'], ['C08.618.326', 'C23.888.852.371'], ['E05.318.308.985.550.325', 'N01.224.935.698.201', 'N06.850.505.400.975.550.325', 'N06.850.520.308.985.550.325'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C04.588.894.797.520', 'C08.381.540', 'C08.785.520'], ['M01.060.116.630'], ['C04.588.274.761', 'C04.588.322.475', 'C06.301.761', 'C06.689.667', 'C19.344.421'], ['C08.381.746', 'C14.907.355.350.700'], ['E01.370.350.700.730'], ['E01.370.350.710', 'E01.370.384.730']]
|
['Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]', 'Organisms [B]', 'Named Groups [M]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 0
|
[Analysis of the Gamete-Fusion Genes in the Haploid-inducing ZMS-P Maize Line].
|
This article is devoted to the study of the double fertilization mechanism in plants, in particular of
the maize gamete membrane fusion genes. We detected and analyzed for the first time gamete-fusion genes
in the maize genome. Using the BLAST program, we searched for the hap2 gene (generative cell specific 1
(gcs1)) homologs from Arabidopsis in the maize genome. The ZM_BFb0162K03 maize transcript was found,
which had 67% identity to the Athap2 gene and contained a conserved region similar to the Athap2 gene fragment.
In mRNA samples from the haploid-inducing and control maize lines, an PCR was conducted by using
primers specific to the ZM_BFb0162K03 sequence fragment. Sequences of the PCR products from a fragment
(1467 bp) of the Zm_hap2 gene of the haploid-inducing and the control maize lines were identical and
also were identical to the maize sequences from the GenBank (ZM_BFb0162K03). PCR products (656 bp
region of Zm_hap2) for the ZM_BFb0162K03 (1925 bp) maize sequence were observed for the cDNA of pollen
grains, ovary, leaves, and roots of the haploid-inducing and control maize lines. Using the Blastx program,
we found significant homology of the maize translated proteins to the GEX2, TET11, and TET12 proteins,
involved in Arabidopsis gamete-fusion contacts.
|
['Arabidopsis', 'Carrier Proteins', 'Germ Cells, Plant', 'Haploidy', 'Plant Proteins', 'Zea mays']
| 30,277,363
|
[['B01.650.940.800.575.912.250.157.100'], ['D12.776.157'], ['A11.497.248', 'A18.024.249.500.249'], ['G05.700.456'], ['D12.776.765'], ['B01.650.940.800.575.912.250.822.966']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[Method of determining disease severity in women with pelvic varicose veins].
|
BACKGROUND: Using assessment scales in clinical and research practice is one of fundamental reference methods of evaluation in human pathological states. Pelvic vein varicosity is an independent nosological entity within the framework of chronic vein diseases. Currently, the clinical methods of assessment in the aspect of a patient-oriented approach in this type of disease are largely understudied and still not duly defined.AIM: The study was aimed at analyzing clinical outcomes of surgical treatment in the form of resection of the ovarian vein in female patients with pelvic varicose veins, based on the developed specialized scale of clinical assessment of disease severity.PATIENTS AND METHODS: We carried out an open prospective study of efficacy of resection of the ovarian vein in 37 women with pelvic varicose veins. The main criterion for assessment was a clinical method of determining manifestations of the disease by means of the Pelvic Venous Clinical Severity Score.RESULTS: According to the Pelvic Venous Clinical Severity Score, improvement of the condition was observed in 36 (97.3%) operated female patients and 1 (2.7%) woman turned out to have negative dynamics. The median of the composite score of the severity scale decreased form 11.78±5.06 points to 5.22±3.19 (p<0.05). The total positive gradient of the score amounted to 6.57±3 .65 points. A significant decrease in manifestations of severity was observed for 9 of the 10 clinical descriptors of the disease.CONCLUSION: The use of the suggested scale in practical assessment of the results made it possible to prove high efficacy of resection of the ovarian vein in women with pelvic varicose veins in the form of decreased intensity of the disease's symptomatology. The VCSS is an easy-to-fill-in tool, taking up little time, ensuring no influence of the physician's personality on the answers, presenting quantitative expression of therapeutic results.
|
['Female', 'Humans', 'Pelvis', 'Prospective Studies', 'Severity of Illness Index', 'Treatment Outcome', 'Varicose Veins', 'Veins', 'Venous Insufficiency']
| 31,503,250
|
[['B01.050.150.900.649.313.988.400.112.400.400'], ['A01.923.600'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['E05.318.308.980.438.475.456.500', 'N05.715.360.300.800.438.375.364.500', 'N06.850.520.308.980.438.475.364.500'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['C14.907.927'], ['A07.015.908'], ['C14.907.952']]
|
['Organisms [B]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Diseases [C]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
House-dust mite nasal provocation: a diagnostic tool in perennial rhinitis.
|
BACKGROUND: In perennial allergic rhinitis (PAR), the skin-prick test (SPT) is a good diagnostic tool to identify the specific allergens. A nasal provocation test (NPT) is used to identify allergens and to confirm the diagnosis. The aim of this study was to determine the optimal cutoff values of symptom and peak nasal inspiratory flow (PNIF) changes after dust-mite NPT for predicting PAR. We also studied the relationship of the changes of symptoms in NPT and the wheal size of SPT.METHODS: One hundred five patients with perennial rhinitis underwent the NPT to Dermatophagoides pteronyssinus and the SPT. The NPT was assessed by changes in symptoms and PNIF. The optimal cutoff values of the symptoms score and PNIF changes after the NPT for predicting the SPT were determined using a receiver operating characteristic (ROC) curve. The relationship of the wheal sizes of SPT and the changes from the NPT were analyzed.RESULTS: Forty-eight patients had a positive SPT to D. pteronyssinus, of whom 33 patients had a positive NPT by increases of the symptom score. Twenty patients had a positive NPT by decreases of PNIF. The area under the ROC curve was 0.85 for symptom score changes and it was 0.612 for PNIF changes. There was a significant correlation between the wheal size of the SPT and symptom changes in the NPT.CONCLUSION: Nasal provocation is a valuable test to confirm the diagnosis of D. pteronyssinus allergy, especially when the wheal from the SPT is small. The symptom change after the house-dust mite NPT is better than the PNIF change for predicting the PAR.
|
['Adolescent', 'Animals', 'Antigens, Dermatophagoides', 'Feasibility Studies', 'Female', 'Humans', 'Inspiratory Capacity', 'Male', 'Middle Aged', 'Nasal Provocation Tests', 'Predictive Value of Tests', 'Pyroglyphidae', 'Reference Standards', 'Respiratory Function Tests', 'Rhinitis, Allergic, Perennial', 'Sensitivity and Specificity', 'Skin Tests']
| 20,338,112
|
[['M01.060.057'], ['B01.050'], ['D23.050.181'], ['E05.318.372.550', 'E05.337.675', 'N05.715.360.330.550', 'N06.850.520.450.550'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.386.700.485.750.900.350', 'G09.772.850.970.500'], ['M01.060.116.630'], ['E01.370.386.550'], ['E05.318.370.800.650', 'N05.715.360.325.700.640', 'N06.850.520.445.800.650'], ['B01.050.500.131.166.132.419.600'], ['E05.978.808'], ['E01.370.386.700'], ['C08.460.799.315.500', 'C08.674.453.500', 'C09.603.799.315.500', 'C20.543.480.680.443.500'], ['E05.318.370.800', 'E05.318.740.872', 'G17.800', 'N05.715.360.325.700', 'N05.715.360.750.725', 'N06.850.520.445.800', 'N06.850.520.830.872'], ['E01.370.225.812.871', 'E05.200.812.871', 'E05.478.594.890']]
|
['Named Groups [M]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Malpractice in diagnosis and treatment of craniocerebral injuries in cases reviewed by the Forensic Medicine Department, Medical University of ??d?.
|
BACKGROUND AND PURPOSE: The aim of the study was to determine the most frequent errors in medical treatment of craniocerebral injuries, based on materials reviewed by the Forensic Medicine Department, Medical University of ??d?, Poland.MATERIAL AND METHODS: Legal opinions in the area of craniocerebral injuries, elaborated by the Forensic Medicine Department, Medical University of ??d?, from 2000 to 2004, were assessed.RESULTS: Seven hundred ninety three opinions related to medical practice were given between 2000 and 2004; 30 cases referred to craniocerebral injuries. There were 19 opinions analyzed in which incorrectness of diagnostic and therapeutic process was found. Eight cases were related to disqualification from hospitalization, 4 cases referred to delay in diagnosis because of transportation to another place, and 4 cases were related to abandoned or misinterpreted imaging studies.CONCLUSIONS: Analyzed material comprised 17 errors during the decision-making process, including 4 diagnostic errors, as well as 1 therapeutic, 1 executive, and 1 organisational error. The most common error was disqualification from hospitalization of patients who should be observed in hospital. It was followed (in order of frequency) by errors related to transportation to the sobering chamber or to another hospital, and failure to perform or misinterpretation of imaging studies. The fewest errors referred to treatment. The main cause of craniocerebral injury was fall of a drunk person, and the alcohol intoxication made diagnosis difficult and delayed.
|
['Academic Medical Centers', 'Craniocerebral Trauma', 'Diagnosis, Differential', 'Disability Evaluation', 'Disease-Free Survival', 'Female', 'Forensic Medicine', 'Humans', 'Kaplan-Meier Estimate', 'Male', 'Malpractice', 'Medical Errors', 'Poland', 'Professional Misconduct', 'Retrospective Studies']
| 17,874,341
|
[['N02.278.020'], ['C10.900.300', 'C26.915.300'], ['E01.171'], ['E01.370.400'], ['E01.789.800.190', 'E05.318.740.998.300', 'N04.761.559.590.800.190', 'N05.715.360.575.575.800.190', 'N05.715.360.750.795.300', 'N06.850.520.830.998.300'], ['H02.403.330', 'I01.198.780.937'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.740.998.650', 'N05.715.360.750.795.650', 'N06.850.520.830.998.650'], ['I01.880.604.583.524', 'N03.706.535.606'], ['N02.421.450'], ['Z01.542.248.679'], ['K01.752.566.479.915', 'N05.350.979'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825']]
|
['Health Care [N]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Organisms [B]', 'Geographicals [Z]', 'Humanities [K]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 1
| 1
|
HIV-1 drug-resistant mutations and related risk factors among HIV-1-positive individuals experiencing treatment failure in Hebei Province, China.
|
BACKGROUND: To understand HIV-1 drug resistance in 11 prefectures of Hebei Province, China, we implemented a cross-sectional HIV-1 molecular epidemiological survey.METHODS: Blood samples were collected from 122 newly diagnosed drug-na?ve HIV-1-positive individuals and 229 antiretroviral therapy (ART)-failure individuals from 11 prefectures in Hebei Province, China. Patient demographic data were obtained via face-to-face interviews using a standardized questionnaire when blood samples were collected. Genotyping of HIV-1 drug resistance (DR) was implemented using an in-house assay.RESULTS: In this study, the overall prevalence of HIV-1 DR was 35.5%. The prevalence of HIV-1 DR in participants experiencing treatment failure and ART-na?ve participants was 51.9 and 5.9%, respectively. Mutations in protease inhibitors, nucleoside reverse transcriptase inhibitors (NRTIs), and non-NRTI (NNRTIs), as well as dual and multiple mutations were extensively seen in participants experiencing treatment failure. The proportions of NNRTI mutations (÷2 = 9.689, p = 0.002) and dual mutations in NRTIs and NNRTIs (÷2 = 39.958, p < 0.001) in participants experiencing treatment failure were significantly higher than those in ART-na?ve participants. The distributions of M184V/I and M41L mutations differed significantly among three main HIV-1 genotypes identified. Viral load, symptoms in the past 3 months, CD4 counts, transmission route, and the duration of ART were found to be associated with HIV-1 DR.CONCLUSIONS: Our results suggest that new prevention and control strategies should be formulated according to the epidemic characteristics of HIV-1-resistant strains in Hebei Province, where antiretroviral drugs are widely used.
|
['Adolescent', 'Adult', 'Aged', 'Anti-HIV Agents', 'Child', 'China', 'Cross-Sectional Studies', 'Drug Resistance, Viral', 'Female', 'HIV Infections', 'HIV-1', 'Humans', 'Male', 'Middle Aged', 'Mutation', 'Prevalence', 'Risk Factors', 'Treatment Failure', 'Viral Load', 'Young Adult']
| 28,114,955
|
[['M01.060.057'], ['M01.060.116'], ['M01.060.116.100'], ['D27.505.954.122.388.077.088'], ['M01.060.406'], ['Z01.252.474.164'], ['E05.318.372.500.875', 'N05.715.360.330.500.875', 'N06.850.520.450.500.875'], ['G06.225.420', 'G06.920.225', 'G07.690.773.984.269.420'], ['C01.221.250.875', 'C01.221.812.640.400', 'C01.778.640.400', 'C01.925.782.815.616.400', 'C01.925.813.400', 'C20.673.480'], ['B04.820.650.589.650.350.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['G05.365.590'], ['E05.318.308.985.525.750', 'N01.224.935.597.750', 'N06.850.505.400.975.525.750', 'N06.850.520.308.985.525.750'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['E01.789.800.760', 'N04.761.559.590.800.760', 'N05.715.360.575.575.800.760'], ['E01.370.225.875.950', 'E05.200.875.950', 'G06.920.850'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Dynamic Reorganization of Functional Connectivity Reveals Abnormal Temporal Efficiency in Schizophrenia.
|
Emerging evidence suggests that schizophrenia is associated with brain dysconnectivity. Nonetheless, the implicit assumption of stationary functional connectivity (FC) adopted in most previous resting-state functional magnetic resonance imaging (fMRI) studies raises an open question of schizophrenia-related aberrations in dynamic properties of resting-state FC. This study introduces an empirical method to examine the dynamic functional dysconnectivity in patients with schizophrenia. Temporal brain networks were estimated from resting-state fMRI of 2 independent datasets (patients/controls = 18/19 and 53/57 for self-recorded dataset and a publicly available replication dataset, respectively) by the correlation of sliding time-windowed time courses among regions of a predefined atlas. Through the newly introduced temporal efficiency approach and temporal random network models, we examined, for the first time, the 3D spatiotemporal architecture of the temporal brain network. We found that although prominent temporal small-world properties were revealed in both groups, temporal brain networks of patients with schizophrenia in both datasets showed a significantly higher temporal global efficiency, which cannot be simply attributable to head motion and sampling error. Specifically, we found localized changes of temporal nodal properties in the left frontal, right medial parietal, and subcortical areas that were associated with clinical features of schizophrenia. Our findings demonstrate that altered dynamic FC may underlie abnormal brain function and clinical symptoms observed in schizophrenia. Moreover, we provide new evidence to extend the dysconnectivity hypothesis in schizophrenia from static to dynamic brain network and highlight the potential of aberrant brain dynamic FC in unraveling the pathophysiologic mechanisms of the disease.
|
['Adolescent', 'Adult', 'Aged', 'Atlases as Topic', 'Brain', 'Connectome', 'Female', 'Humans', 'Magnetic Resonance Imaging', 'Male', 'Middle Aged', 'Nerve Net', 'Schizophrenia', 'Time Factors', 'Young Adult']
| 29,878,254
|
[['M01.060.057'], ['M01.060.116'], ['M01.060.116.100'], ['L01.178.682.192.836.147'], ['A08.186.211'], ['E01.370.350.578.875.500.249', 'E01.370.376.537.625.500.249', 'E05.629.875.500.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.350.825.500'], ['M01.060.116.630'], ['A08.511'], ['F03.700.750'], ['G01.910.857'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Information Science [L]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 1
| 1
| 0
| 0
|
[Acceptance of transsexualism among university students from L?d?].
|
UNLABELLED: Transsexualism is one of the gender identity disorders where psychological sex is opposed to anatomical sex. This disorder leads to a discrepancy between the preferred social gender and the biological sex.AIM: The aim of this research is to compare knowledge and attitude toward transsexualism in student's opinion, coming from three universities in L?d?.METHOD: The questionnaire study was performed in the group of 300 students from three universities in L?d?: Technical University of L?d?, University of L?d?, Medical University of L?d?. The questionnaire contained 30 questions related to respondent's sex, birthplace, knowledge about definition and aetiology of transsexualism and also rights which students would grant to transsexuals.RESULTS: The right definition of transsexualism was pointed by 64% of students from Medical University, 57% from Technical University and 40% from University of L?d?. The right to surgical sex change for transsexuals would be granted by 87% of students from the Medical University, 69% from the University of L?d? and 40% from the Technical University. Majority of medical students (90%) and respectively 78% and 57% from the University of L?d? and Technical University would accept a transsexual as his/her co-worker.CONCLUSIONS: Student's knowledge about transsexualism is similar and does not differ from a foreign student's knowledge. Students from natural science studies (medicine and biology) are the most tolerant towards transsexuals.
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['Adult', 'Female', 'Health Knowledge, Attitudes, Practice', 'Humans', 'Interpersonal Relations', 'Male', 'Peer Group', 'Poland', 'Social Environment', 'Social Perception', 'Stereotyping', 'Students', 'Surveys and Questionnaires', 'Transsexualism', 'Universities']
| 18,567,410
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[['M01.060.116'], ['F01.100.150.500', 'N05.300.150.410'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F01.829.401'], ['F01.829.316.483'], ['Z01.542.248.679'], ['I01.880.853.500'], ['F02.463.593.752'], ['F01.100.920', 'F01.145.813.854'], ['M01.848'], ['E05.318.308.980', 'N05.715.360.300.800', 'N06.850.520.308.980'], ['F01.145.802.975.750'], ['I02.783.830', 'J03.832.830']]
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['Named Groups [M]', 'Psychiatry and Psychology [F]', 'Health Care [N]', 'Organisms [B]', 'Geographicals [Z]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Technology, Industry, and Agriculture [J]']
| 0
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| 0
| 0
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| 0
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