Title
stringlengths 1
395
⌀ | abstractText
stringlengths 57
5.98k
| meshMajor
stringlengths 14
1.03k
| pmid
int64 22
33.2M
| meshid
stringlengths 2
3.14k
| meshroot
stringlengths 2
421
| A
int64 0
1
| B
int64 0
1
| C
int64 0
1
| D
int64 0
1
| E
int64 0
1
| F
int64 0
1
| G
int64 0
1
| H
int64 0
1
| I
int64 0
1
| J
int64 0
1
| L
int64 0
1
| M
int64 0
1
| N
int64 0
1
| Z
int64 0
1
|
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
TEs or not TEs? That is the evolutionary question.
|
Transposable elements (TEs) have contributed a wide range of functional sequences to their host genomes. A recent paper in BMC Molecular Biology discusses the creation of new transcripts by transposable element insertion upstream of retrocopies and the involvement of such insertions in tissue-specific post-transcriptional regulation.
|
['Animals', 'Base Sequence', 'Conserved Sequence', 'DNA Transposable Elements', 'Databases, Genetic', 'Drosophila melanogaster', 'Evolution, Molecular', 'Gene Deletion', 'Gene Dosage', 'Gene Duplication', 'Gene Expression Regulation', 'Genes, Plant', 'Genetic Speciation', 'Genome', 'Genome, Human', 'Humans', 'Models, Genetic', 'Mutagenesis, Insertional', 'Phylogeny']
| 19,863,763
|
[['B01.050'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['G02.111.570.580'], ['D13.444.308.520', 'G02.111.570.080.708.330.200', 'G05.360.080.708.330.200', 'G05.360.340.024.425.200'], ['L01.313.500.750.300.188.400.325', 'L01.470.750.750.325'], ['B01.050.500.131.617.720.500.500.750.310.250.500'], ['G05.045.250', 'G16.075.250'], ['G05.365.590.762.320', 'G05.558.800.320'], ['G05.380.350'], ['G05.365.590.320', 'G05.558.320'], ['G05.308'], ['G05.360.340.024.340.393', 'G05.360.340.365.500'], ['G05.045.350', 'G16.075.350'], ['G05.360.340'], ['G05.360.340.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.599.395.397'], ['E05.393.420.601.550', 'G05.365.590.575', 'G05.558.550'], ['G05.697', 'G16.075.605', 'L01.100.697']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Does sedentary behavior increase the risk of low back pain? A population-based co-twin study of Spanish twins.
|
BACKGROUND: The relationship between sedentary lifestyle and low back pain (LBP) remains unclear and previous research has not accounted for genetic and early environmental factors.PURPOSE: Our aim was to investigate if sedentary behavior is associated with the lifetime prevalence of persistent LBP and the risk of developing persistent LBP, care-seeking due to LBP, and activity limiting LBP when genetics and early environmental factors are accounted for.STUDY DESIGN: Both cross-sectional and longitudinal designs with a within-pair twin case-control were implemented.PATIENT SAMPLE: There were 2,148 twins included in the cross-sectional analysis whereas 1,098 twins free of persistent LBP at baseline were included in the longitudinal analysis.OUTCOME MEASURES: Sedentary behavior was the explanatory variable. Lifetime prevalence of LBP was the outcome variable in the cross-sectional analysis. The incidence of persistent LBP, care-seeking due to LBP, and activity limiting LBP were the outcome variables for the longitudinal analysis.METHODS: This observational study was supported by a grant in 2012. No competing interests were declared.RESULTS: In the cross-sectional analysis, sedentary behavior was slightly associated with an increased prevalence of persistent LBP in females but not in males. This association was not apparent when genetics and early environmental factors were accounted for. We acknowledge that the small sample included in the co-twin analyses have yielded wide confidence intervals, and that caution should be exercised when interpreting and an association may not be ruled out. In the longitudinal analysis, sedentary behavior did not significantly increase the risk of persistent LBP, care-seeking due to LBP, or activity limiting LBP.CONCLUSIONS: Sedentary behavior is associated with concurrent LBP. However, this association is weak; it only appears in females and decreases when accounting for genetics. Future studies using a twin design with larger samples should be conducted to further test these findings.
|
['Case-Control Studies', 'Female', 'Gene-Environment Interaction', 'Humans', 'Low Back Pain', 'Male', 'Middle Aged', 'Sedentary Behavior', 'Spain', 'Twins']
| 28,232,052
|
[['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['G05.695.337'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C23.888.592.612.107.400'], ['M01.060.116.630'], ['F01.145.749', 'F01.829.458.705'], ['Z01.542.846'], ['M01.438.873']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Diseases [C]', 'Named Groups [M]', 'Psychiatry and Psychology [F]', 'Geographicals [Z]']
| 0
| 1
| 1
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Reasons for readmission after carotid endarterectomy.
|
OBJECTIVE: With increasing oversight of postoperative outcomes with the Patient Protection and Affordable Care Act, the reduction of readmissions is necessary to avoid financial penalties. This article provides a multi-institutional, multivariate analysis of the pre- and postoperative patient factors associated with readmission after carotid endarterectomy (CEA).METHODS: Using the National Surgical Quality Improvement Program from 2011, we considered 8456 patients. The primary outcome variable was 30-day unplanned readmission. Multiple logistic regression was used, and we controlled for preoperative demographic variables, comorbidities and clinical characteristics, and postoperative medical and surgical complications.RESULTS: Patients with CEA had a 6.0% unplanned readmission rate. The most common comorbidities in the readmitted patients included hypertension, diabetes, and bleeding disorder. Risk-adjusted multiple regression indicated that preoperative bleeding disorder (odds ratio [OR] 1.62), diabetes (OR 1.46), history of a cerebrovascular accident/stroke (OR 1.46), and increasing age (OR 1.01) were statistically significant predictors for readmission. Postoperatively, surgical-site infection (OR 21.90), myocardial infarction (OR 10.35), sepsis/septic shock (OR 7.79), cerebrovascular accident/stroke (OR 6.58), pneumonia (OR 4.37), and urinary tract infection (OR 3.21) were associated with a greater rate of readmission.CONCLUSIONS: Readmission after CEA occurs at a comparatively high rate. Preoperative bleeding disorders, diabetes, cerebrovascular accidents, and age and postoperative surgical-site infection, myocardial infarction, sepsis/septic shock, pneumonia, and cerebrovascular accident were associated with readmission. These findings may help guide the surgical management of patients and prevent costly readmissions.
|
['Aged', 'Benchmarking', 'Comorbidity', 'Endarterectomy, Carotid', 'Female', 'Humans', 'Male', 'Patient Readmission', 'Postoperative Complications', 'Quality Improvement', 'Risk Adjustment', 'Risk Factors', 'Treatment Outcome']
| 23,973,514
|
[['M01.060.116.100'], ['N04.452.500.150', 'N04.761.685.150', 'N04.761.700.150', 'N05.700.150', 'N05.715.360.650.150'], ['N05.715.350.225', 'N06.850.490.687'], ['E04.100.814.456.250'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.760.400.620', 'N02.421.585.400.620'], ['C23.550.767'], ['J01.293.754', 'N04.761.744'], ['N04.452.871.715.800', 'N04.761.789.800', 'N05.715.360.750.625.700.690.800'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Named Groups [M]', 'Health Care [N]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Diseases [C]', 'Technology, Industry, and Agriculture [J]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 1
| 1
| 0
|
Impact of routine percutaneous coronary intervention after out-of-hospital cardiac arrest due to ventricular fibrillation.
|
INTRODUCTION: Since 2003, we have routinely used percutaneous coronary intervention (PCI) and mild therapeutic hypothermia (MTH) to treat patients < 80 years of age after out-of-hospital cardiac arrest (OHCA) related to ventricular fibrillation. The aim of our study was to evaluate the prognostic impact of routine PCI in association with MTH and the potential influence of age.METHODS: We studied 111 consecutive patients resuscitated successfully following OHCA related to shock-sensitive rhythm. They were divided into five groups according to age: < 45 years (n = 22, group 1), 45 to 54 years (n = 27, group 2), 55 to 64 years (n = 22, group 3), 65 to 74 years (n = 23, group 4) and ?75 years (n = 17, group 5). Emergency coronary angiography was performed in hemodynamically stable patients < 80 years old, regardless of the electrocardiogram pattern. MTH was targeted to a core temperature of 32°C to 34°C for 24 hours.RESULTS: Most patients (73%) had coronary heart disease, although its incidence in group 1 was lower than in other groups (41% versus 81%; P = 0.01). In group 1, all patients but one underwent coronary angiography, and 33% of them underwent associated PCI. In group 5, only 53% of patients underwent a coronary angiography and 44% underwent PCI. Overall in-hospital survival was 54%, ranging between 52% and 64% in groups 1 to 4 and 24% in group 5. Time from collapse to return of spontaneous circulation was associated with mortality (odds ratio (OR) = 1.05 (25th to 75th percentile range, 1.03 to 1.08); P < 0.001), whereas PCI was associated with survival (OR = 0.30 (25th to 75th percentile range, 0.11 to 0.79); P = 0.01).CONCLUSIONS: We suggest that routine coronary angiography with potentially associated PCI may favorably alter the prognosis of resuscitated patients with stable hemodynamics who are treated with MTH after OHCA related to ventricular fibrillation. Although age was not an independent cause of death, the clinical relevance of this therapeutic strategy remains to be determined in older people.
|
['Adult', 'Age Factors', 'Aged', 'Cardiopulmonary Resuscitation', 'Coronary Angiography', 'Female', 'Humans', 'Hypothermia, Induced', 'Male', 'Middle Aged', 'Out-of-Hospital Cardiac Arrest', 'Prognosis', 'Treatment Outcome', 'Ventricular Fibrillation']
| 21,569,361
|
[['M01.060.116'], ['N05.715.350.075', 'N06.850.490.250'], ['M01.060.116.100'], ['E02.365.647.110'], ['E01.370.350.130.625', 'E01.370.350.700.060.200', 'E01.370.370.050.200', 'E01.370.370.380.200'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.258.750'], ['M01.060.116.630'], ['C14.280.383.610'], ['E01.789'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['C14.280.067.922', 'C23.550.073.922']]
|
['Named Groups [M]', 'Health Care [N]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
A naturally occurring substitution in human immunodeficiency virus Tat increases expression of the viral genome.
|
A natural amino acid substitution in the human immunodeficiency virus type 1 (HIV-1) transcriptional activator Tat increases its activity and compensates for deleterious mutations elsewhere in the Tat protein. Substitution of asparagine for threonine 23 increases Tat transactivation of the HIV-1 promoter and the binding of Tat to the cellular kinase positive transcription elongation factor b (P-TEFb). Of nine other position 23 mutations tested, only the serine substitution retained wild-type activity. Correspondingly, asparagine is the most frequent amino acid at this position in HIV-1 isolates, followed by threonine and serine. Asparagine is prevalent in Tat proteins of viruses in clades A, C, and D, which are major etiologic agents of AIDS. We suggest that selection for asparagine in position 23 confers an advantage to the virus, since it can compensate for deleterious mutations in Tat. It may also support the replication of otherwise less fit drug-resistant viruses and permit the emergence of virulent strains.
|
['Amino Acid Sequence', 'Amino Acid Substitution', 'Asparagine', 'Gene Products, tat', 'Genetic Variation', 'Genome, Viral', 'HIV-1', 'Humans', 'Leukocytes, Mononuclear', 'Molecular Sequence Data', 'Positive Transcriptional Elongation Factor B', 'Protein-Serine-Threonine Kinases', 'Transcription, Genetic', 'Transcriptional Activation', 'tat Gene Products, Human Immunodeficiency Virus']
| 12,857,933
|
[['G02.111.570.060', 'L01.453.245.667.060'], ['E05.393.420.601.035', 'G05.558.109'], ['D12.125.068.060', 'D12.125.095.165', 'D12.125.154.049'], ['D12.776.260.755.199', 'D12.776.930.900.199', 'D12.776.964.900.750.750', 'D12.776.964.925.984.400'], ['G05.365'], ['G05.360.340.358.840'], ['B04.820.650.589.650.350.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A11.118.637.555', 'A15.145.229.637.555', 'A15.382.490.555'], ['L01.453.245.667'], ['D08.811.913.696.620.682.700.626', 'D12.776.930.955.500'], ['D08.811.913.696.620.682.700'], ['G02.111.873', 'G05.297.700'], ['G05.308.800'], ['D12.776.260.755.199.500', 'D12.776.930.900.199.500', 'D12.776.964.775.562.773', 'D12.776.964.900.750.750.500', 'D12.776.964.925.984.400.500']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Assessment of cadmium content of potato grown in Weining County, Guizhou Province, China.
|
A total of 54 soil and 54 potato samples have been collected from Weining County to evaluate the accumulation of cadmium in potatoes. The concentrations of the total Cd and the available Cd in the soil samples have been detected. The total concentrations of Cd were from 0.41 to 10.0 mg/kg with an average value of 2.60 mg/kg in soil. The concentrations of available Cd in the soil were 0.07 to 3.47 mg/kg with an average value of 0.59 mg/kg. The concentration of the available Cd showed a good linear positive correlation with the total Cd content in the soil. For the 54 potato samples, the Cd concentrations were from 0.023 to 0.18 mg/kg with an average value 0.083 mg/kg (fresh weight).The bioconcentration factor (BCF) values of Cd in potatoes, based on dry weight, were from 0.02 to 0.96 with an average value 0.24. The uptake of cadmium by plants is dependent on various soil and environmental factors. A regression model to predict the concentration of cadmium in Weining potatoes based on soil properties and elevation was developed. The results showed the elevation and the soil pH played an important role and had a negative influence on the uptake of Cd by potato in Weining County. The mean intake of Cd by adults through consumption of potato from Weining would be 5.9 ìg/day, and it is well below the provisionally tolerable daily intake for Cd (70 ìg/day).
|
['Cadmium', 'China', 'Environmental Monitoring', 'Models, Theoretical', 'Soil', 'Soil Pollutants', 'Solanum tuberosum']
| 28,432,509
|
[['D01.268.556.137', 'D01.268.956.061', 'D01.552.544.137'], ['Z01.252.474.164'], ['N06.850.460.350.080', 'N06.850.780.375'], ['E05.599'], ['D20.721', 'G01.311.820', 'G16.500.275.815', 'N06.230.600'], ['D27.888.284.756'], ['B01.650.940.800.575.912.250.908.500.725.777']]
|
['Chemicals and Drugs [D]', 'Geographicals [Z]', 'Health Care [N]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
|
Evaluation of Optical Coherence Tomography Meibography in Patients With Obstructive Meibomian Gland Dysfunction.
|
PURPOSE: To evaluate optical coherence tomography meibography (OCT-M) in patients with and without obstructive meibomian gland dysfunction (MGD) and to determine the relationship between OCT-M and ocular surface clinical tests.METHODS: Twenty-two patients with MGD and 16 control subjects were included. Each patient underwent an evaluation of ocular surface disease symptoms, lid margin abnormality score, lipid layer thickness evaluation, and meibomian gland (MG) morphological changes using noncontact infrared meibography and OCT-M. OCT-M scans were acquired in 4 different locations. OCT-M parameters including the MG length and width and palpebral conjunctival thickness were evaluated.RESULTS: Within the OCT-M parameters, the mean length and width of MGs were significantly decreased in patients with MGD (310 ± 60 ìm and 214 ± 30 ìm, respectively) compared with the control group (361 ± 53 ìm, P = 0.041 and 264 ± 41 ìm, P = 0.021, respectively). The mean conjunctival thickness was also significantly increased in patients with MGD (448 ± 68 ìm) than in the control group (356 ± 59 ìm, P = 0.03). The mean length of MGs was correlated with symptoms (r = 0.34, P = 0.034), and the mean MG width was correlated with tear film breakup time (r = -0.412, P = 0.009) and the lid margin abnormality score (r = 0.334, P = 0.038).CONCLUSIONS: The MG length and width were significantly decreased in patients with MGD than in the control group. These parameters were correlated to both ocular surface symptoms and clinical signs. This new imaging technique may be a useful tool for MGD evaluation.
|
['Adult', 'Diagnostic Techniques, Ophthalmological', 'Eyelid Diseases', 'Female', 'Humans', 'Male', 'Meibomian Glands', 'Middle Aged', 'Observer Variation', 'Tears', 'Tomography, Optical', 'Tomography, Optical Coherence', 'Young Adult']
| 26,226,467
|
[['M01.060.116'], ['E01.370.380'], ['C11.338'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A09.371.337.614', 'A10.336.827.600'], ['M01.060.116.630'], ['E01.354.753', 'N02.421.450.600', 'N05.715.350.150.675', 'N06.850.490.500.250'], ['A12.200.882'], ['E01.370.350.589.249', 'E01.370.350.825.805', 'E05.642.249'], ['E01.370.350.589.249.500', 'E01.370.350.825.805.500', 'E05.642.249.500'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Organisms [B]', 'Anatomy [A]', 'Health Care [N]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Cytochrome c is dispensable for fas-induced caspase activation and apoptosis.
|
Cytochrome c is thought to play an important role in the initiation of apoptosis following its release from mitochondria. It is controversial whether such release is also involved in caspase activation and apoptotic cell death after ligation of the cell surface molecule Fas. We addressed this issue by investigating cells from the human cell lines Jurkat and SKW6 which had been treated with the inhibitor of the mitochondrial F0/F1-ATPase, oligomycin. Oligomycin-treatment led, over a wide range of concentrations, to ATP-depletion and, at similar concentrations, abrogated the appearance of caspase-3-like activity caused by stauroporine. Electroporation of cytochrome c protein into intact cells induced caspase activation in both cell lines and significant nuclear apoptosis in Jurkat cells. In ATP-depleted cells, electroporation of cytochrome c induced neither caspase activation nor nuclear fragmentation. Fas-induced caspase activation and nuclear apoptosis, however, were unaffected by the depletion of ATP. Thus, cytochrome c is unlikely to be an important factor in Fas-induced cell death.
|
['Adenosine Triphosphate', 'Apoptosis', 'Caspases', 'Coumarins', 'Cytochrome c Group', 'Dose-Response Relationship, Drug', 'Electroporation', 'Enzyme Activation', 'Humans', 'Jurkat Cells', 'Oligomycins', 'Oligopeptides', 'Proton-Translocating ATPases', 'Signal Transduction', 'Staurosporine', 'Tumor Cells, Cultured', 'fas Receptor']
| 10,405,325
|
[['D03.633.100.759.646.138.236', 'D13.695.667.138.236', 'D13.695.827.068.236'], ['G04.146.954.035'], ['D08.811.277.656.262.500.126', 'D08.811.277.656.300.200.126', 'D12.644.360.075.405', 'D12.776.476.075.405'], ['D03.383.663.283.446', 'D03.633.100.150.446'], ['D08.244.286', 'D12.776.422.220.286'], ['G07.690.773.875', 'G07.690.936.500'], ['E05.200.500.454', 'E05.242.448', 'E05.301.500'], ['G02.111.263', 'G03.328'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A11.251.210.190.495', 'A11.251.860.180.495', 'A15.382.490.555.567.569.440'], ['D02.540.505.620'], ['D12.644.456'], ['D08.811.277.040.025.325', 'D08.811.913.696.650.150.500', 'D12.776.157.530.450.250.875.500', 'D12.776.543.585.450.250.875.500'], ['G02.111.820', 'G04.835'], ['D03.132.436.750', 'D03.633.100.473.144.750', 'D03.633.100.473.402.750', 'D03.633.100.496.500.500.750', 'D03.633.300.148.750'], ['A11.251.860'], ['D12.776.543.750.690.500', 'D12.776.543.750.705.852.760.195']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Pediatric lung transplantation for cystic fibrosis.
|
Pediatric lung transplantation for cystic fibrosis has uncertain survival effects. Three retrospective studies disagree on survival benefit. The US Lung Allocation Score has changed patient selection for lung transplantation but confounds the analysis of survival outcomes. A prospective trial is needed to resolve clinical equipoise and explore quality-of-life effects.
|
['Child', 'Cystic Fibrosis', 'Graft Survival', 'Humans', 'Lung Transplantation', 'Patient Selection', 'Treatment Outcome']
| 18,791,441
|
[['M01.060.406'], ['C06.689.202', 'C08.381.187', 'C16.320.190', 'C16.614.213'], ['G12.875.545.340'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E04.928.600.495', 'E04.936.450.495'], ['E05.581.500.653', 'N04.590.731'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Named Groups [M]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Monte Carlo calculation of the wall correction factors for ionization chambers and Aeq for 60Co gamma rays.
|
The application of cavity-ionization chambers to the standadization of 60Co gamma-ray beams, in terms of exposure, requires that the specific ionization of air Jg, be corrected for the attenuation and scatter of the incident rays by the wall, central electrode, and supporting stem of the chamber. A Monte Carlo photon-electron transport code has been developed for the purpose of calculating this correction for spherical and cylindrical chambers. The code has been applied to a spherical graphite chamber having dimensions typical of the chambers used by the NBS, the calculated wall-correction factor is in close agreement with the average of the NBS factors which were determined experimentally. The code was also used to calculate Aeq, which is central to the determination of tissue-air ratios. The calculated value, 0.989 +/- 0.003, is very close to the generally accepted value, 0.985.
|
['Cobalt Radioisotopes', 'Electron Transport', 'Gamma Rays', 'Monte Carlo Method', 'Nuclear Reactors', 'Operations Research', 'Radionuclide Generators', 'Scattering, Radiation']
| 713,976
|
[['D01.268.556.185.500.354', 'D01.268.956.155.500.354', 'D01.496.239.354', 'D01.496.749.256', 'D01.552.544.185.500.354'], ['G02.111.248', 'G03.295.531.403', 'G03.493.350'], ['G01.358.500.505.300', 'G01.750.250.300', 'G01.750.750.400'], ['E05.318.740.525', 'L01.906.394.422', 'N05.715.360.750.540', 'N06.850.520.830.525'], ['E07.710.600'], ['H01.770.644.333', 'L01.906.394'], ['E07.710.770'], ['E05.196.822', 'G01.867']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Information Science [L]', 'Health Care [N]', 'Disciplines and Occupations [H]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 0
|
Lower Magnetization Transfer Ratio in the Forceps Minor Is Associated with Poorer Gait Velocity in Older Adults.
|
BACKGROUND AND PURPOSE: Gait disturbances in the elderly are disabling and a major public health issue but are poorly understood. In this multimodal MR imaging study, we used 2 voxel-based analysis methods to assess the voxelwise relationship of magnetization transfer ratio and white matter hyperintensity location with gait velocity in older adults.MATERIALS AND METHODS: We assessed 230 community-dwelling participants of the Austrian Stroke Prevention Family Study. Every participant underwent 3T MR imaging, including magnetization transfer imaging. Voxel-based magnetization transfer ratio-symptom mapping correlated the white matter magnetization transfer ratio of each voxel with gait velocity. To assess a possible relationship between white matter hyperintensity location and gait velocity, we applied voxel-based lesion-symptom mapping.RESULTS: We found a significant association between the magnetization transfer ratio within the forceps minor and gait velocity (â = 0.134; 95% CI, 0.011-0.258; P = .033), independent of demographics, general physical performance, vascular risk factors, and brain volume. White matter hyperintensities did not significantly change this association.CONCLUSIONS: Our study provides new evidence for the importance of magnetization transfer ratio changes in gait disturbances at an older age, particularly in the forceps minor. The histopathologic basis of these findings is yet to be determined.
|
['Adult', 'Aged', 'Brain', 'Female', 'Gait', 'Gait Disorders, Neurologic', 'Humans', 'Magnetic Resonance Imaging', 'Male', 'Middle Aged']
| 27,979,793
|
[['M01.060.116'], ['M01.060.116.100'], ['A08.186.211'], ['E01.370.600.250', 'G11.427.410.568.900.750'], ['C10.597.404', 'C23.888.592.413'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.350.825.500'], ['M01.060.116.630']]
|
['Named Groups [M]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Organisms [B]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Immunogenicity and Safety of an EB66 Cell-Culture-Derived Influenza A/Indonesia/5/2005(H5N1) AS03-Adjuvanted Vaccine: A Phase 1 Randomized Trial.
|
BACKGROUND: Cell-culture-derived (CC) influenza vaccine production methods could provide benefits over classical embryonated-egg technology, including a higher production capacity and the faster creation of a supply that meets demand.METHODS: A CC-inactivated split-virus influenza A/Indonesia/5/2005(H5N1) vaccine derived from the EB66 cell line (hereafter, "CC-H5N1") was investigated in a phase 1 randomized, blinded study. Healthy adults (n = 521) received 2 vaccine doses (days 0 and 21) of either investigational CC-H5N1 vaccine (1.9 µg or 3.75 µg of hemagglutinin antigen [HA] with the AS03 adjuvant system or 15 µg of plain HA), embryonated-egg-derived vaccines (3.75 µg of HA with AS03 or 15 µg of plain HA), or placebo. Assessment of the adjuvant effect and immunogenicity was performed using Center for Biologics Evaluation and Research acceptability criteria 21 days after dose 2. Safety was assessed until month 12.RESULTS: AS03-adjuvanted CC-H5N1 elicited a homologous hemagglutination inhibition antibody response that satisfied immunogenicity criteria 21 days after dose 2 and persisted at month 12. Adjuvant effect and immune response against a drift-variant strain were demonstrated. No vaccine-related serious adverse events were reported. The immunogenicity and safety of the CC-H5N1 formulation containing 3.75 µg of HA and AS03 appeared to be similar to those for the licensed egg-derived AS03-adjuvanted control vaccine.CONCLUSIONS: The feasibility of the EB66 cell line to produce an immunogenic influenza vaccine with acceptable safety profile was demonstrated. Antigen sparing was achieved through combination with AS03 adjuvant. This CC-H5N1 might contribute to the rapid access of vaccine in the event of an influenza A(H5N1) pandemic.CLINICAL TRIALS REGISTRATION: NCT01236040.
|
['Adjuvants, Immunologic', 'Adolescent', 'Adult', 'Animals', 'Antibodies, Viral', 'Cell Line', 'Drug Combinations', 'Ducks', 'Embryonic Stem Cells', 'Female', 'Humans', 'Influenza A Virus, H5N1 Subtype', 'Influenza Vaccines', 'Influenza, Human', 'Male', 'Middle Aged', 'Polysorbates', 'Single-Blind Method', 'Squalene', 'Young Adult', 'alpha-Tocopherol']
| 25,722,291
|
[['D27.505.696.477.067'], ['M01.060.057'], ['M01.060.116'], ['B01.050'], ['D12.776.124.486.485.114.254', 'D12.776.124.790.651.114.254', 'D12.776.377.715.548.114.254'], ['A11.251.210'], ['D26.310'], ['B01.050.150.900.248.050.200', 'B01.050.150.900.248.690.345'], ['A11.872.700.250'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['B04.820.480.968.405.400.500'], ['D20.215.894.899.302'], ['C01.748.310', 'C01.925.782.620.365', 'C08.730.310'], ['M01.060.116.630'], ['D02.033.455.250.700.690', 'D05.750.741.700', 'D25.720.741.700', 'J01.637.051.720.741.700'], ['E05.318.370.850', 'N05.715.360.325.730', 'N06.850.520.445.850'], ['D02.455.326.271.665.806', 'D02.455.849.919.681'], ['M01.060.116.815'], ['D03.383.663.283.909.750.249', 'D03.633.100.150.909.750.249']]
|
['Chemicals and Drugs [D]', 'Named Groups [M]', 'Organisms [B]', 'Anatomy [A]', 'Diseases [C]', 'Technology, Industry, and Agriculture [J]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 1
| 1
| 0
|
Phenotypical characteristics of group B streptococcus in parturients.
|
Colonization by Group B Streptococcus (GBS) is highly prevalent among pregnant women, with prevalence rates ranging between 4% and 30%. The infection may be transmitted vertically and may result in serious neonatal consequences. In the period from November 2003 to May 2004, a cross-sectional study was carried out among 316 parturients at the Jundia? Teaching Hospital to establish the prevalence of genital GBS colonization, to identify the factors associated with colonization and the characteristic phenotypes of these streptococci. Samples from rectal and vaginal areas were collected for selective culture in Todd-Hewitt broth. Susceptibility to 7 antimicrobial agents was tested using the antibiotic diffusion disk technique, and the isolated strains were classified using specific antisera. The prevalence of GBS colonization was 14.6%. No strain was resistant to penicillin, ampicillin, erythromycin or nitrofurantoin. The majority of strains were sensitive to cephalothin. Greatest resistance was to gentamicin (76.1%), followed by clindamycin (17.4%). The most frequent serotype was Ib (23.9%), followed by serotypes II and Ia (19.6% and 17.4%, respectively). There was no correlation between serotype and greater antimicrobial resistance. In conclusion, the prevalence of GBS in parturients was high and penicillin continues to be the drug of choice for intrapartum prophylaxis. The most frequent serotype (Ib) found in this study differs from those found in the majority of studies carried out in other countries, revealing the need to identify prevalent serotypes in each region so that specific vaccines can be designed.
|
['Adult', 'Anti-Bacterial Agents', 'Carrier State', 'Cross-Sectional Studies', 'Female', 'Humans', 'Microbial Sensitivity Tests', 'Phenotype', 'Pregnancy', 'Pregnancy Complications, Infectious', 'Rectum', 'Risk Factors', 'Serotyping', 'Streptococcal Infections', 'Streptococcus agalactiae', 'Vagina']
| 17,625,774
|
[['M01.060.116'], ['D27.505.954.122.085'], ['N06.850.520.169'], ['E05.318.372.500.875', 'N05.715.360.330.500.875', 'N06.850.520.450.500.875'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.225.875.595', 'E05.200.875.595', 'E05.337.550.400'], ['G05.695'], ['G08.686.784.769'], ['C01.674', 'C13.703.700'], ['A03.556.124.526.767', 'A03.556.249.249.767'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['E01.370.225.812.742', 'E01.370.225.875.150.125.890', 'E05.200.812.742', 'E05.200.875.150.125.890', 'E05.478.594.780'], ['C01.150.252.410.890'], ['B03.353.750.737.872.100', 'B03.510.400.800.872.100', 'B03.510.550.737.872.100'], ['A05.360.319.779']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Health Care [N]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Decisional capacity of depressed elderly to consent to electroconvulsive therapy.
|
The purpose of this article is to determine the abilities of severely depressed elderly to consent to electroconvulsive therapy (ECT) and to investigate the impact of educational intervention on their capacity. Forty severely depressed adults referred for ECT, with Mini-Mental State Examination scores greater than 20, were recruited. Using the MacArthur Competence Assessment Tool for Treatment (MacCAT-T), decisional capacities were assessed at baseline and reassessed after education. Between the 2 assessments, all subjects received standard education, and half of the group was subsequently randomized to receive further education. At baseline, the geriatric group scored lower on understanding, reasoning, and choice and higher on appreciation. After education, all MacCAT-T scores increased for both age groups. Depressed elderly in the sample, as a group, had adequate decisional capacities to consent to ECT. They showed greater improvement in decisional capacity with education. The findings highlight the importance of providing education to the elderly to optimize their ability to give informed consent.
|
['Adult', 'Aged', 'Aged, 80 and over', 'Bipolar Disorder', 'Decision Making', 'Depressive Disorder, Major', 'Electroconvulsive Therapy', 'Female', 'Humans', 'Informed Consent', 'Male', 'Mental Competency', 'Mental Status Schedule', 'Middle Aged', 'Patient Education as Topic', 'Patient Participation', 'Psychiatric Department, Hospital', 'Rehabilitation Centers', 'Treatment Outcome']
| 15,018,698
|
[['M01.060.116'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['F03.084.500'], ['F02.463.785.373'], ['F03.600.300.375'], ['F04.570.200.583', 'F04.669.224.300'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['I01.880.604.473.650.718', 'I01.880.604.583.427', 'N03.706.437.650.312', 'N03.706.535.489'], ['F01.590', 'F02.410', 'I01.880.604.583.530', 'N03.706.535.625'], ['F04.711.513.603.500', 'F04.711.513.653.574'], ['M01.060.116.630'], ['I02.233.332.500', 'N02.421.726.407.680'], ['F01.100.150.750.500.620', 'F01.145.488.887.500.620', 'N02.421.143.212.300', 'N03.540.245.360.300', 'N05.300.150.800.500.620'], ['N02.278.216.500.968.641', 'N04.452.442.452.422.641'], ['N02.278.808'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Named Groups [M]', 'Psychiatry and Psychology [F]', 'Organisms [B]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 0
|
IL-5 receptor-mediated tyrosine phosphorylation of SH2/SH3-containing proteins and activation of Bruton's tyrosine and Janus 2 kinases.
|
Interleukin 5 (IL-5) induces proliferation and differentiation of B cells and eosinophils by interacting with its receptor (IL-5R) which consists of two distinct polypeptide chains, alpha and beta (beta c). Although both IL-5R alpha and beta c lack a kinase catalytic domain, IL-5 is capable of inducing tyrosine phosphorylation of cellular proteins. We investigated the role of IL-5R alpha in tyrosine phosphorylation of molecules involved in IL-5 signal transduction, using an IL-5-dependent early B cell line, Y16 and transfectants expressing intact or mutant IL-5R alpha together with intact beta c. The results revealed that the transfectants expressing truncated IL-5R alpha, which entirely lacks a cytoplasmic domain, together with beta c, showed neither protein-tyrosine phosphorylation nor proliferation in response to IL-5. This confirms that IL-5R alpha plays a critical role in protein-tyrosine phosphorylation which triggers cell growth. IL-5 stimulation results in rapid tyrosine phosphorylation of beta c and proteins containing Src homology 2 (SH2) and/or SH3 domains such as phosphatidyl-inositol-3 kinase, Shc, Vav, and HS1, suggesting their involvement in IL-5-mediated signal transduction. IL-5 stimulation significantly enhanced activities of Janus 2 and B cell-specific Bruton's tyrosine kinases (JAK2 and Btk) and increased the tyrosine phosphorylation of JAK2 kinase. These results and recent data on signaling of growth factors taken together, multiple biochemical pathways driven by tyrosine kinases such as JAK2 and Btk are involved in IL-5 signal transduction.
|
['Agammaglobulinaemia Tyrosine Kinase', 'Animals', 'Antibodies, Monoclonal', 'B-Lymphocytes', 'Benzoquinones', 'Cell Line', 'Cricetinae', 'Enzyme Activation', 'Humans', 'Interleukin-5', 'Janus Kinase 1', 'Janus Kinase 2', 'Kinetics', 'Lactams, Macrocyclic', 'Lymphocyte Activation', 'Macromolecular Substances', 'Mice', 'Phosphoproteins', 'Phosphorylation', 'Phosphotyrosine', 'Protein-Tyrosine Kinases', 'Proto-Oncogene Proteins', 'Quinones', 'Receptors, Interleukin', 'Receptors, Interleukin-5', 'Rifabutin', 'Transfection', 'Tyrosine']
| 7,525,847
|
[['D08.811.913.696.620.682.725.025'], ['B01.050'], ['D12.776.124.486.485.114.224', 'D12.776.124.790.651.114.224', 'D12.776.377.715.548.114.224'], ['A11.063.438', 'A11.118.637.555.567.562', 'A15.145.229.637.555.567.562', 'A15.382.032.438', 'A15.382.490.555.567.562'], ['D02.806.250'], ['A11.251.210'], ['B01.050.150.900.649.313.992.635.075.250'], ['G02.111.263', 'G03.328'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.644.276.374.465.202', 'D12.776.467.374.465.186', 'D23.529.374.465.202'], ['D08.811.913.696.620.682.725.124.100', 'D12.776.476.393.100'], ['D08.811.913.696.620.682.725.124.200', 'D12.776.476.393.200', 'D12.776.624.664.700.117'], ['G01.374.661', 'G02.111.490'], ['D02.065.589.327', 'D04.345.295'], ['E01.370.225.812.482', 'E05.200.812.482', 'E05.478.594.530', 'G12.450.050.400.545', 'G12.565'], ['D05'], ['B01.050.150.900.649.313.992.635.505.500'], ['D12.776.744'], ['G02.111.665', 'G02.607.780', 'G03.796'], ['D12.125.072.050.875.750', 'D12.125.740.740'], ['D08.811.913.696.620.682.725'], ['D12.776.624.664.700'], ['D02.806'], ['D12.776.543.750.705.852.420'], ['D12.776.543.750.705.852.420.380'], ['D03.633.400.811.650', 'D04.345.295.750.650'], ['E05.393.350.810', 'G05.728.860'], ['D12.125.072.050.875']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
One, two-, and three-level instrumented posterolateral fusion of the lumbar spine with a local bone graft: a prospective study with a 2-year follow-up.
|
STUDY DESIGN: Prospective trial.OBJECTIVE: To examine the difference in bone union and clinical results after one-, two-, and three-level instrumented posterolateral fusion surgery using a local bone graft.SUMMARY OF BACKGROUND DATA: The iliac crest bone graft technique for lumbar posterolateral fusion surgery is widely used; however, donor site problems such as pain and sensory disturbance have been reported. Local bone has been used for fusion surgery; however, its reliability as a graft for multiple segments has not been fully reported.METHODS: One hundred twenty-two patients diagnosed with degenerated spondylolisthesis were divided into three groups [spondylolisthesis at 1 level (n = 42), at 2 levels (n = 40), and at 3 levels (n = 40)]. All patients underwent decompression and instrumented posterolateral fusion with a local bone graft. The amount of bone graft, proportion of patients with (rate) and duration of bone union, Visual Analog Scale (VAS) score, Japanese Orthopedic Association Score (JOAS), and Oswestry Disability Index (ODI) were evaluated before and 2 years after therapy.RESULTS: VAS score, JOA score, and ODI were not significantly different among the three groups before and after surgery (P > 0.05). Average amount of local bone graft used for one segment significantly decreased in proportion to the number of fusion levels (P < 0.05). The rate of bone union was 88% in the one-level group, 85% in the two-level group, and 62.5% in the three-level group, which was significantly lower than that in the one- and two-level groups (P < 0.05).CONCLUSION: If one- and two-level posterolateral fusion were performed, the local bone graft technique provides a good and uniform bone union rate; however, for three-level fusion poor results were obtained because of an insufficient amount of local bone.
|
['Aged', 'Aged, 80 and over', 'Bone Transplantation', 'Female', 'Follow-Up Studies', 'Humans', 'Lumbar Vertebrae', 'Male', 'Middle Aged', 'Pain Measurement', 'Prospective Studies', 'Sacrum', 'Spinal Fusion']
| 21,224,776
|
[['M01.060.116.100'], ['M01.060.116.100.080'], ['E02.095.147.725.052', 'E04.555.130', 'E04.936.580.052'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A02.835.232.834.519'], ['M01.060.116.630'], ['E01.370.600.550.324'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['A02.835.232.834.717'], ['E04.555.100.700']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Anatomy [A]']
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
A methodological investigation of the Intermodal Preferential Looking paradigm: Methods of analyses, picture selection and data rejection criteria.
|
The Intermodal Preferential Looking paradigm provides a sensitive measure of a child's online word comprehension. To complement existing recommendations (Fernald, Zangl, Portillo, & Marchman, 2008), the present study evaluates the impact of experimental noise generated by two aspects of the visual stimuli on the robustness of familiar word recognition with and without mispronunciations: the presence of a central fixation point and the level of visual noise in the pictures (as measured by luminance saliency). Twenty-month-old infants were presented with a classic word recognition IPL procedure in 3 conditions: without a fixation stimulus (No Fixation - noisiest condition), with a fixation stimulus before trial onset (Fixation, intermediate), and with a fixation stimulus, a neutral background and equally salient images (Fixation Plus - least noisy). Data were systematically analyzed considering a range of data selection criteria and dependent variables (proportion of looking time towards the target, longest look, and time-course analysis). Critically, the expected pronunciation and naming interaction was only found in the Fixation Plus condition. We discuss the impact of data selection criteria and the dependent variable choice on the modulation of these effects across the different conditions.
|
['Acoustic Stimulation', 'Comprehension', 'Female', 'Fixation, Ocular', 'Humans', 'Infant', 'Infant Behavior', 'Language', 'Male', 'Noise', 'Photic Stimulation', 'Recognition, Psychology', 'Reproducibility of Results', 'Research Design']
| 26,176,183
|
[['E02.037', 'E02.190.888.030', 'E05.723.136'], ['F02.463.188.357'], ['G14.350.253'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703'], ['F01.145.179.500'], ['F01.145.209.399', 'L01.559'], ['G01.750.770.776.567', 'G16.500.275.600', 'N06.230.400', 'N06.850.460.610'], ['E05.723.729'], ['F02.463.425.540.706'], ['E05.318.370.725', 'E05.337.851', 'N05.715.360.325.685', 'N06.850.520.445.725'], ['E05.581.500', 'H01.770.644.728']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Named Groups [M]', 'Information Science [L]', 'Health Care [N]', 'Disciplines and Occupations [H]']
| 0
| 1
| 0
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 1
| 1
| 0
|
Elements of resilience after the World Trade Center disaster: reconstituting New York City's Emergency Operations Centre.
|
In this paper we examine the reconstitution of the Emergency Operations Centre (EOC) after its destruction in the World Trade Center attack, using that event to highlight several features of resilience. The paper summarises basic EOC functions, and then presents conceptions of resilience as understood from several disciplinary perspectives, noting that work in these fields has sought to understand how a natural or social system that experiences disturbance sustains its functional processes. We observe that, although the physical EOC facility was destroyed, the organisation that had been established to manage crises in New York City continued, enabling a response that drew on the resources of New York City and neighbouring communities, states and the federal government. Availability of resources--which substituted for redundancy of personnel, equipment and space--pre-existing relationships that eased communication challenges as the emergency developed and the continuation of organisational patterns of response integration and role assignments were among the factors that contributed to resilience following the attack.
|
['Aircraft', 'Crisis Intervention', 'Disaster Planning', 'Emergency Medical Services', 'Humans', 'Interinstitutional Relations', 'New York City', 'Rescue Work', 'Terrorism']
| 12,703,151
|
[['J01.937.285.100'], ['F04.754.252'], ['N06.230.100.035'], ['N02.421.297'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['N04.452.822.400'], ['Z01.107.567.875.350.530.530', 'Z01.107.567.875.500.530.530', 'Z01.433.741'], ['N06.230.100.350'], ['I01.198.240.856.800', 'I01.880.735.900.800']]
|
['Technology, Industry, and Agriculture [J]', 'Psychiatry and Psychology [F]', 'Health Care [N]', 'Organisms [B]', 'Geographicals [Z]', 'Anthropology, Education, Sociology, and Social Phenomena [I]']
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 1
| 1
|
Phase II and pharmacodynamic studies of pyrazine diazohydroxide (NSC 361456) in patients with advanced renal and colorectal cancer.
|
Pyrazine diazohydroxide (PZDH) is a novel antitumor agent that forms DNA adducts via the reactive pyrazine diazonium ion. In a recent Phase I study of PZDH, we identified a recommended Phase II dose of 100 mg/m2/day x 5, given as a 5-min i.v. bolus with the cycles repeated every 42 days (N. J. Vogelzang, et al, Cancer Res., 54: 114-119, 1994). There was a moderate negative correlation between serum chloride concentration and logarithm platelet nadir, suggesting the hypothesis that PZDH is activated in an acidic environment, leading to more toxicity in acidotic patients. Therefore, the University of Chicago Phase II cooperative network conducted two Phase II studies of PZDH in renal cancer (15 patients, 2 with liver metastases) and in 5-fluorouracil-refractory colorectal cancer (14 patients, 13 with liver metastases) to determine efficacy in each disease and to correlate safety and tolerance of the drug with PZDH pharmacokinetics/pharmacodynamics and with arterial blood gas measurements. There were no responses seen in either tumor type. The primary toxicity of PZDH was myelosuppression with neutropenia (absolute neutrophil count, < 1000/microl) and thrombocytopenia (<50,000 cells/microl), seen in 41 and 24% of all cycles, respectively. Other grade 3 and 4 toxicities were rare. Pharmacodynamic analysis revealed no significant correlation between plasma levels at 5, 60, and 120 min; WBCs; absolute neutrophil and platelet count nadirs; and initial serum chloride or blood pH levels. The colorectal patients experienced significantly more thrombocytopenia than did the renal cancer patients (median platelet nadir after cycle 1 was 151 x 10(3)/microl for renal patients versus 76 x 10(3)/microl for colon patients; P = 0.04), suggesting either that prior 5-fluorouracil and leucovorin reduced bone marrow reserve or that colorectal patients with liver metastases experienced more PZDH toxicity. Regression analyses revealed a possible relationship (P = 0.06) between serum pH and thrombocytopenia (i.e., for each increase of 0.03 in pH, there was a 34% increase in the platelet nadir), but there was no relationship between serum chloride and thrombocytopenia. Curiously, an increase in alkaline phosphatase was associated with an increase in the platelet nadir (P = 0.02). If PZDH continues to be developed as an antineoplastic agent, further studies of these relationships are suggested.
|
['Adult', 'Aged', 'Aged, 80 and over', 'Antineoplastic Agents', 'Cell Count', 'Colorectal Neoplasms', 'Female', 'Humans', 'Kidney Neoplasms', 'Liver Neoplasms', 'Male', 'Middle Aged', 'Neoplasm Invasiveness', 'Pyrazines', 'Survival Rate']
| 9,563,886
|
[['M01.060.116'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['D27.505.954.248'], ['E01.370.225.500.195', 'E05.200.500.195', 'E05.242.195', 'G04.140'], ['C04.588.274.476.411.307', 'C06.301.371.411.307', 'C06.405.249.411.307', 'C06.405.469.158.356', 'C06.405.469.491.307', 'C06.405.469.860.180'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C04.588.945.947.535', 'C12.758.820.750', 'C12.777.419.473', 'C13.351.937.820.535', 'C13.351.968.419.473'], ['C04.588.274.623', 'C06.301.623', 'C06.552.697'], ['M01.060.116.630'], ['C04.697.645', 'C23.550.727.645'], ['D03.383.679'], ['E05.318.308.985.550.900', 'N01.224.935.698.826', 'N06.850.505.400.975.550.900', 'N06.850.520.308.985.550.900']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Organisms [B]', 'Health Care [N]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Dietary exposure to acrylamide in a group of Japanese adults based on 24-hour duplicate diet samples.
|
Acrylamide is a probable human carcinogen and known human neurotoxin that can be generated in food through heating. Using a mathematical modelling approach, our previous study estimated long-term average dietary exposure to acrylamide in the Japanese people; however, the validity of these estimates remained unknown. Here, we aimed to obtain a more accurate estimate of acrylamide exposure that would reflect the usual practice of heat processing and consumption of foods in the population. We collected duplicate diet samples and dietary records during 24 h from a group of Japanese adults. A total of 110 duplicate diet samples were analysed for acrylamide by LC-MS/MS. Data from individual dietary records were used to examine the association between dietary acrylamide exposure and consumption of selected food groups (e.g., coffee, tea, confectioneries, and vegetables prepared at high temperature [deep-frying, stir-frying, saut?ing, and baking]). Of the 110 homogenised diet samples, 108 contained detectable levels of acrylamide. Dietary exposure to acrylamide ranged from 8 to 1582 ng/kg body weight (bw)/day, with the mean value of 215 ng/kg-bw/day and median value of 143 ng/kg-bw/day. This mean value was higher than the value we previously estimated for Japanese adults using a mathematical approach. Multiple linear regression analysis showed log dietary acrylamide exposure was significantly associated with consumption of coffee and vegetables prepared at high temperature during 24-hr of sampling (adj. R2 = 0.250, p < 0.001). We revealed significant difference in dietary acrylamide exposure between participants who had coffee and vegetables prepared at high temperature (median, 169 ng/kg-bw/day; range, 35-1224 ng/kg-bw/day, n = 42) and those who had none of them (median, 75 ng/kg-bw/day; range, 8-311 ng/kg-bw/day, n = 15) (Steel-Dwass test, p < 0.05).
|
['Acrylamide', 'Adult', 'Aged', 'Diet', 'Dietary Exposure', 'Female', 'Food Contamination', 'Healthy Volunteers', 'Humans', 'Japan', 'Male', 'Middle Aged', 'Vegetables', 'Young Adult']
| 30,650,020
|
[['D02.065.122.015', 'D02.241.081.069.094.015'], ['M01.060.116'], ['M01.060.116.100'], ['G07.203.650.240'], ['N06.850.460.350.040'], ['J01.576.423.850.730.500.249', 'N06.850.460.400', 'N06.850.601.500.249'], ['M01.774.500', 'M01.955.236'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['Z01.252.474.463', 'Z01.639.595'], ['M01.060.116.630'], ['B01.650.160.956', 'B01.650.510.956', 'G07.203.300.850', 'J02.500.850'], ['M01.060.116.815']]
|
['Chemicals and Drugs [D]', 'Named Groups [M]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Technology, Industry, and Agriculture [J]', 'Organisms [B]', 'Geographicals [Z]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 1
| 1
|
[Geosystemic zoning as a basis for rehabilitation environmental measures of mining and smelting complex (exemplified by metallurgic plant)].
|
The article covers a method for zoning of natural technogenic geosystem (exemplified by territory of ash-heap from metallurgic enterprise - one of the major metallurgic enterprises in Russia). The method is based on main principles of systemic approach and enables objective evaluation of space-time spread of pollution for effective management solutions. The studied territory appeared to be assigned to "dangerous" pollution category and requires specification and implementation of effective nature protection measures, with primary step of systematic monitoring of all environmental components including biota - that enables consideration of its results in geosystem studies and gives information for forecasting ecologic state of environment.
|
['Conservation of Natural Resources', 'Environmental Monitoring', 'Environmental Pollution', 'Metallurgy', 'Mining', 'Russia']
| 30,351,783
|
[['J01.256', 'N06.230.080'], ['N06.850.460.350.080', 'N06.850.780.375'], ['N06.850.460'], ['J01.576.655.875.400'], ['J01.576.655.875.500'], ['Z01.252.122.500', 'Z01.542.248.775']]
|
['Technology, Industry, and Agriculture [J]', 'Health Care [N]', 'Geographicals [Z]']
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 1
|
Quantitative detection of Helicobacter pylori in water samples by real-time PCR amplification of the cag pathogenicity island gene, cagE.
|
AIMS: A new real-time PCR assay that simultaneously amplifies a 102-bp fragment of the cagE gene from Helicobacter pylori and a new internal positive control containing a specific sequence of the gyrB gene from Aeromonas hydrophila, was developed and validated for the detection of H. pylori in environmental samples.METHODS AND RESULTS: The specificity, limits of detection and quantification, repeatability, reproducibility, and accuracy of the method were calculated. The resulting values confirmed the applicability of the method for the quantitative detection of H. pylori. The feasibility of the method was also evaluated by testing 13 pyloric antrum-positive biopsies and 69 water samples, including potable (10), surface (19) and wastewater (40) matrices. The results showed that all the biopsies and 3 of the 40 wastewater samples analysed were positive.CONCLUSIONS: This real-time PCR method provides a sensitive, specific, and accurate method for the rapid quantification of H. pylori in environmental samples.SIGNIFICANCE AND IMPACT OF THE STUDY: The PCR diagnostic system proposed in this work, provides a suitable tool for the quantitative detection of H. pylori in environmental samples and can be useful for verifying the role of water as a potential route of its transmission.
|
['Bacterial Proteins', 'DNA Primers', 'DNA, Bacterial', 'Helicobacter pylori', 'Limit of Detection', 'Polymerase Chain Reaction', 'Sensitivity and Specificity', 'Waste Disposal, Fluid', 'Water Microbiology', 'Water Supply']
| 19,302,298
|
[['D12.776.097'], ['D13.695.578.424.450.275', 'D27.720.470.530.600.223.600'], ['D13.444.308.212'], ['B03.440.500.550', 'B03.660.150.235.500.250.550'], ['E05.318.740.872.374', 'N05.715.360.750.725.500', 'N06.850.520.830.872.500'], ['E05.393.620.500'], ['E05.318.370.800', 'E05.318.740.872', 'G17.800', 'N05.715.360.325.700', 'N05.715.360.750.725', 'N06.850.520.445.800', 'N06.850.520.830.872'], ['N06.850.780.200.800.800.890', 'N06.850.860.510.900.600.900'], ['H01.158.273.540.274.777', 'N06.850.425.450'], ['J01.293.821.500']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Disciplines and Occupations [H]', 'Technology, Industry, and Agriculture [J]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
|
[Spinal meningeal melanocytoma simulating neurinoma: case report].
|
Meningeal melanocytomas are infrequent tumors that when located in the spinal cord and because of their close relationship to the nerve root can resemble a neurinoma. The MRl can help to differentiate them from the neurinomas preoperatively. The case of a female patient harboring a cervical meningeal melanocytoma involving the C7 nerve root, and diagnosed preoperatively as an hourglass neurinoma is presented.
|
['Adolescent', 'Diagnosis, Differential', 'Female', 'Humans', 'Magnetic Resonance Imaging', 'Meningeal Neoplasms', 'Meningioma', 'Neurilemmoma']
| 12,444,412
|
[['M01.060.057'], ['E01.171'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.350.825.500'], ['C04.588.614.250.580', 'C10.551.240.500'], ['C04.557.580.520', 'C04.557.645.520', 'C04.588.614.250.580.500', 'C10.551.240.500.500'], ['C04.557.465.625.650.595', 'C04.557.580.600.610.595', 'C04.557.580.625.650.595']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
[Effect of neuroactivating amino acids on the system activating sympathetic preganglionic neurons].
|
In anesthetized cats, the bioelectrical responses were recorded in T3 white ramus during stimulation of intercostal nerve of the T3 segment and of descending pathways (dorsolateral funiculus) of the C5 segment. The effects of GABA, glycine (and its synaptic antagonists picrotoxin and strychnine), and of glutamic acid on different components of the responses were not identical which suggested different sites of the activity effects of glutamic acid and of the inhibitory effects of GABA and glycine in the central pathways for activation of the sympathetic preganglionic neurons.
|
['Aminobutyrates', 'Animals', 'Autonomic Fibers, Preganglionic', 'Cats', 'Glutamates', 'Glycine', 'Interneurons', 'Muscles', 'Neurons, Afferent', 'Picrotoxin', 'Reaction Time', 'Reflex', 'Spinal Cord', 'Strychnine', 'Sympathetic Nervous System', 'Vasomotor System', 'gamma-Aminobutyric Acid']
| 658,543
|
[['D02.241.081.114.500', 'D12.125.190'], ['B01.050'], ['A08.675.127.500.060', 'A08.675.542.234.060', 'A08.800.050.050.060', 'A08.800.800.060.060', 'A11.671.188.500.060', 'A11.671.501.234.060'], ['B01.050.150.900.649.313.750.377.750.250.125'], ['D12.125.067.625', 'D12.125.119.409'], ['D12.125.481'], ['A08.675.358', 'A11.671.358'], ['A02.633', 'A10.690'], ['A08.675.650', 'A11.671.650'], ['D02.455.426.392.368.367.800', 'D02.540.552'], ['E05.796.817', 'F02.830.650', 'F04.669.817', 'G11.561.677'], ['E01.370.376.550.650', 'E01.370.600.550.650', 'F02.830.702', 'G11.561.731'], ['A08.186.854'], ['D03.132.436.681.722', 'D03.633.100.473.402.681.722', 'D03.633.100.496.500.500.681.722'], ['A08.800.050.800'], ['A08.800.050.800.900'], ['D02.241.081.114.500.350', 'D12.125.190.350']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Use of the Krusen Limb Load Monitor to quantify temporal and loading measurements of gait.
|
The purposes of this investigation were to determine whether the temporal and force measurements from the Krusen Limb Load Monitor produced clinically reliable data and to begin identifying the factors that determine the monitor's reliability. Temporal and loading measurements were made from the output of the Krusen Limb Load Monitor and compared to values obtained from a calibrated force platform. Such comparisons were made for 30 steps taken by two subjects on three separate occasions and from the same two subjects plus a third subject for 100 consecutive steps. For most measures, mean values from the limb-load monitor were significantly different from those recorded from the force platform. From a clinical perceptive, however, the range of measures was narrow for the 95 percent confidence level of the observed differences for the temporal components of stance between the limb-load monitor and force platform, with the narrowest range of measures related to the appropriateness of "fit" of the limb-load monitor force plate within the shoe. The loading components of stance showed a relatively wide 95 percent confidence interval that appeared unrelated to fit. Thus, given a "good fitting" force plate insert, the therapist can make clinically meaningful measurements of the temporal components of the stance phase of gait using the limb-load monitor.
|
['Biomechanical Phenomena', 'Female', 'Gait', 'Humans', 'Male', 'Physical Therapy Modalities']
| 7,089,061
|
[['G01.154.090', 'G01.374.089'], ['E01.370.600.250', 'G11.427.410.568.900.750'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.779', 'E02.831.535']]
|
['Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]']
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
The regeneration potential after human and autologous stem cell transplantation in a rat sciatic nerve injury model can be monitored by MRI.
|
Traumatic nerve injuries are a major clinical challenge. Tissue engineering using a combination of nerve conduits and cell-based therapies represents a promising approach to nerve repair. The aim of this study was to examine the regeneration potential of human adipose-derived stem cells (hASCs) after transplantation in a nonautogenous setting and to compare them with autogenous rat ASCs (rASCs) for early peripheral nerve regeneration. Furthermore, the use of MRI to assess the continuous process of nerve regeneration was elaborated. The sciatic nerve injury model in female Sprague-Dawley rats was applied, and a 10-mm gap created by using a fibrin conduit seeded with the following cell types: rASCs, Schwann cell (SC)-like cells from rASC, rat SCs (rSCs), hASCs from the superficial and deep abdominal layer, as well as human stromal vascular fraction (1 ? 10(6) cells). As a negative control group, culture medium only was used. After 2 weeks, nerve regeneration was assessed by immunocytochemistry. Furthermore, MRI was performed after 2 and 4 weeks to monitor nerve regeneration. Autogenous ASCs and SC-like cells led to accelerated peripheral nerve regeneration, whereas the human stem cell groups displayed inferior results. Nevertheless, positive trends could be observed for hASCs from the deep abdominal layer. By using a clinical 3T MRI scanner, we were able to visualize the graft as a small black outline and small hyperintensity indicating the regenerating axon front. Furthermore, a strong correlation was found between the length of the regenerating axon front measured by MRI and the length measured by immunocytochemistry (r = 0.74, p = 0.09). We successfully transplanted and compared human and autologous stem cells for peripheral nerve regeneration in a rat sciatic nerve injury model. Furthermore, we were able to implement the clinical 3T MRI scanner to monitor the efficacy of cellular therapy over time.
|
['Adipose Tissue', 'Animals', 'Disease Models, Animal', 'Female', 'Fibrin', 'Humans', 'Immunohistochemistry', 'Magnetic Resonance Imaging', 'Nerve Regeneration', 'Peripheral Nerve Injuries', 'Radiography', 'Rats', 'Rats, Sprague-Dawley', 'Schwann Cells', 'Sciatic Nerve', 'Stem Cell Transplantation', 'Stem Cells', 'Tissue Engineering', 'Transplantation, Autologous', 'Transplantation, Heterologous']
| 24,380,629
|
[['A10.165.114'], ['B01.050'], ['C22.232', 'E05.598.500', 'E05.599.395.080'], ['D12.776.124.270'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.225.500.607.512', 'E01.370.225.750.551.512', 'E05.200.500.607.512', 'E05.200.750.551.512', 'E05.478.583', 'H01.158.100.656.234.512', 'H01.158.201.344.512', 'H01.158.201.486.512', 'H01.181.122.573.512', 'H01.181.122.605.512'], ['E01.370.350.825.500'], ['G11.561.585', 'G16.762.611'], ['C10.668.829.712', 'C10.900.575', 'C26.915.650'], ['E01.370.350.700'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.750'], ['A08.637.800', 'A08.800.800.690', 'A11.650.800'], ['A08.800.800.720.450.760'], ['E02.095.147.500.500', 'E04.936.225.687'], ['A11.872'], ['E05.481.500.311.500', 'J01.293.069.249.500'], ['E04.936.664'], ['E04.936.764']]
|
['Anatomy [A]', 'Organisms [B]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Disciplines and Occupations [H]', 'Phenomena and Processes [G]', 'Technology, Industry, and Agriculture [J]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
|
[Situational profile and intervention strategy in the Mesoamerican region in maternal, neonatal and reproductive health area].
|
To present the main results of the regional situation diagnosis and intervention plan developed in 2010 as part of the planning activities of the Mesoamerican Health System by the Working Group on Maternal, Reproductive and Neonatal Health. A group of experts and representatives from countries in the region (Central America and nine southern Mexican states) conducted an exhaustive review of available data to construct a situational analysis and a review of effective practices for improving maternal, reproductive and neonatal health. Finally, the group proposed a regional action plan, defining regional goals and specific interventions. The situational diagnosis suggests that, although there has been progress in the last 10 years, maternal and neonatal mortality rates are still unnaceptably high in the region, with a substantial variability across countries. The group proposed as a regional goal the reduction of maternal and neonatal mortality in accordance with the Millenium Development Goals. The regional plan recommends specific maternal and neonatal health interventions emphasizing obstetric and neonatal emergency care, skilled birth attendance and family planning. The plan also includes a five year implementation strategy, along with training and evaluation strategies. The regional plan for maternal, neonatal and reproductive health has the potential to be successful, provided it is effectively implemented.
|
['Adolescent', 'Adult', 'Central America', 'Child', 'Child Health Services', 'Developing Countries', 'Family Planning Services', 'Female', 'Goals', 'Health Plan Implementation', 'Health Promotion', 'Health Services Needs and Demand', 'Humans', 'Infant Mortality', 'Infant Welfare', 'Infant, Newborn', 'International Cooperation', 'Maternal Health Services', 'Maternal Mortality', 'Maternal Welfare', 'Mexico', 'Middle Aged', 'Pregnancy', 'Regional Health Planning', 'Reproductive Health', 'Young Adult']
| 22,344,376
|
[['M01.060.057'], ['M01.060.116'], ['Z01.107.169'], ['M01.060.406'], ['N02.421.143.130'], ['I01.615.500.300'], ['N02.421.143.401', 'N02.421.800.249'], ['F01.658.500'], ['N03.349.300'], ['I02.233.332.445', 'N02.421.726.407.579'], ['N03.349.380.420', 'N05.300.450'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.308.985.550.475', 'N01.224.935.698.489', 'N06.850.505.400.975.550.475', 'N06.850.520.308.985.550.475'], ['I01.880.787.539'], ['M01.060.703.520'], ['I01.615.500'], ['N02.421.143.620', 'N02.421.800.500'], ['E05.318.308.985.550.500', 'N01.224.935.698.653', 'N06.850.505.400.975.550.500', 'N06.850.520.308.985.550.500'], ['I01.880.787.678'], ['Z01.107.567.589'], ['M01.060.116.630'], ['G08.686.784.769'], ['N03.349.650'], ['N01.400.625'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Geographicals [Z]', 'Health Care [N]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Psychiatry and Psychology [F]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 0
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 1
|
Effect of genetic variation in LRRTM3 on risk of Alzheimer disease.
|
OBJECTIVE: To explore the role of leucine-rich repeat transmembrane 3 (LRRTM3) in late-onset Alzheimer disease (AD) by independent genetic epidemiologic and functional studies.METHODS: First, we explored associations between LRRTM3 single-nucleotide polymorphisms and AD in the National Institute on Aging Late-Onset Alzheimer's Disease case-control data set (993 patients and 884 control subjects) and a cohort of Caribbean Hispanics (549 patients and 544 controls) using single-marker and haplotype analyses. Then we explored the effect of LRRTM3 small-hairpin RNAs on amyloid precursor protein processing.RESULTS: One single-nucleotide polymorphism in the promoter region (rs16923760; C allele: odds ratio, -0.74, P=.03), and a block of 4 single-nucleotide polymorphisms in intron 2 (rs1925608, C allele: 0.84, P=.04; rs7082306, A allele: 0.75, P=.04; rs1925609, T allele: 1.2, P=.03; and rs10997477, T allele: 0.88, P=.05) were associated with AD in the National Institute on Aging Late-Onset Alzheimer's Disease data set or the Caribbean Hispanic data set. The corresponding haplotypes were also associated with AD risk (.01 < P < .05). In addition, LRRTM3 knockdown with small-hairpin RNAs caused a significant decrease in amyloid precursor protein processing (P < .05 to P < .01) compared with the scrambled small-hairpin RNA condition.CONCLUSIONS: These complementary findings support the notions that genetic variation in LRRTM3 is associated with AD risk and that LRRTM3 may modulate ã-secretase processing of amyloid precursor protein. Additional studies are needed to determine whether the specific alleles associated with differential risk for AD indeed confer this risk through an effect of LRRTM3 expression levels that in turn modulates amyloid precursor protein processing.
|
['Aged', 'Aged, 80 and over', 'Alzheimer Disease', 'Amyloid beta-Protein Precursor', 'Apolipoprotein E3', 'Caribbean Region', 'Chi-Square Distribution', 'Cognition Disorders', 'Female', 'Gene Expression Regulation', 'Genetic Association Studies', 'Genetic Predisposition to Disease', 'HEK293 Cells', 'Hispanic Americans', 'Histone Acetyltransferases', 'Humans', 'Linkage Disequilibrium', 'Lysine Acetyltransferase 5', 'Male', 'Membrane Proteins', 'Mental Status Schedule', 'National Institute on Aging (U.S.)', 'Nerve Tissue Proteins', 'Neuropsychological Tests', 'Nuclear Proteins', 'Polymorphism, Single Nucleotide', 'RNA, Small Interfering', 'Transfection', 'United States']
| 22,393,166
|
[['M01.060.116.100'], ['M01.060.116.100.080'], ['C10.228.140.380.100', 'C10.574.945.249', 'F03.615.400.100'], ['D12.776.049.407.249', 'D12.776.543.039', 'D12.776.645.468.500', 'D12.776.811.050'], ['D10.532.091.500.500', 'D12.776.070.400.500.500', 'D12.776.521.120.500.500'], ['Z01.107.084'], ['E05.318.740.994.300', 'G17.820.300', 'N05.715.360.750.750.200', 'N06.850.520.830.994.300'], ['F03.615.250'], ['G05.308'], ['E05.393.385'], ['C23.550.291.687.500', 'G05.380.355'], ['A11.251.210.172.750', 'A11.436.334'], ['M01.686.754.441'], ['D08.811.913.050.134.415.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G05.348.500'], ['D08.811.913.050.134.415.500.062'], ['D12.776.543'], ['F04.711.513.603.500', 'F04.711.513.653.574'], ['I01.409.418.750.600.650.496.465', 'N03.540.052.750.465', 'N03.540.348.500.500.600.650.496.465'], ['D12.776.631'], ['F04.711.513'], ['D12.776.660'], ['G05.365.795.598'], ['D13.150.650.700', 'D13.444.735.150.700', 'D13.444.735.790.552.875'], ['E05.393.350.810', 'G05.728.860'], ['Z01.107.567.875']]
|
['Named Groups [M]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Chemicals and Drugs [D]', 'Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Anatomy [A]', 'Organisms [B]', 'Anthropology, Education, Sociology, and Social Phenomena [I]']
| 1
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 1
|
Positive effects of cryopreserved adult or fetal liver cell transplants on hypercholesterolemia and hepatic antioxidant defenses in cholesterol-fed rabbits.
|
The liver plays a central role in lipid metabolism and the pathophysiology of many lipid disorders leads in turn to liver cell injury. Adult hepatocyte transplants provide well-recognized metabolic support, whilst hepatic stem cells may promote liver regeneration and repair, but in both cases, any clinical application would require low temperature banking of the cells. A model of dietary hypercholesterolemia was established in rabbits over 5 months, and transplants of cryopreserved adult hepatocytes (CH) and cryopreserved fetal liver cells (CFLC) were compared to Sham transplants. Cryopreservation was performed by a two-step freezing protocol using 1.5mol/l dimethyl sulfoxide (Me(2)SO). Serum contents of cholesterol lipid classes were measured during the subsequent 4 weeks, in addition to markers of serum and liver oxidative stress. Both CH and CFLC transplantation resulted in a decrease of serum lipids during the 1st week after transplantation. The effect of CH was limited to the 1st week, but CFLC provided a sustained lipid-lowering effect over the 4 weeks. The ultimate outcome of CFLC transplantation by the end of 4 weeks was more pronounced and statistically significant for both serum total cholesterol (0.15+/-0.05 versus 3.65+/-1.4mmol/l) and high-density lipoprotein-cholesterol (0.04+/-0.01 versus 0.56+/-0.06mmol/l) compared to Sham transplants (p<0.05 in both cases). CFLC transplantation also normalized hepatic tissue antioxidant defenses, namely an increase in reduced glutathione content, and enzyme activities for catalase and glutathione reductase (all significantly higher at p<0.05 than in Sham transplants) by 4 weeks.
|
['Animals', 'Antioxidants', 'Cell Separation', 'Cryopreservation', 'Fetal Tissue Transplantation', 'Hepatocytes', 'Hypercholesterolemia', 'Lipid Peroxidation', 'Lipids', 'Liver', 'Rabbits']
| 17,624,324
|
[['B01.050'], ['D27.505.519.217', 'D27.505.696.706.125', 'D27.720.799.047'], ['E01.370.225.500.363', 'E05.200.500.363', 'E05.242.363'], ['E01.370.225.500.620.760.160', 'E01.370.225.750.600.760.160', 'E02.792.156', 'E05.200.500.620.760.160', 'E05.200.750.600.760.160', 'E05.760.156'], ['E02.095.147.725.300', 'E04.936.580.300'], ['A11.436.348'], ['C18.452.584.500.500.396'], ['G02.111.515', 'G03.295.531.587'], ['D10'], ['A03.620'], ['B01.050.150.900.649.313.968.700']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Diseases [C]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Short- and long-term effects of a novel paclitaxel coated stent in the porcine coronary model.
|
BACKGROUND: Drug eluting stents (DES) are unique in allowing sustained release for weeks after a single short intervention. The challenge with DES still remaining is the combination of a biocompatible drug-eluting matrix including an antiproliferative drug showing efficacy and safety in restenosis prevention. The aim of the present animal studies was to evaluate the novel paclitaxel coated Coroflex. Please stent in the porcine coronary model.METHODS AND RESULTS: Stents were implanted into LAD and CX arteries of 49 domestic pigs. After 5 days, 4 weeks, 3 months, or 6 months, the animals underwent control angiography including dissection of the stented coronary arteries for histology. After 5 days, 3 and 6 months the Coroflex. Please stent was compared with its uncoated counterpart and a paclitaxel free but polymer coated version. After 28 days, an additional group received the Taxus Express(2) stent. After 5 days, healing with the paclitaxel coated stent was comparable to the uncoated bare metal stent as reflected in a similar neointimal proliferation. Compared to the Taxus stent, the new Coroflex. Please stent showed a similar neointimal proliferation after 4 weeks. Inflammatory reaction was comparable among the bare stent and polymer coated stent groups. Paclitaxel coating was associated with a slightly increased inflammatory reaction with both DES. After 3 and 6 months, all groups showed a similar neointimal proliferation and inflammatory reaction.CONCLUSION: The present porcine studies demonstrate excellent safety of the new paclitaxel coated Coroflex stent in the porcine coronary stent model.
|
['Angioplasty, Balloon, Coronary', 'Animals', 'Coronary Angiography', 'Coronary Disease', 'Coronary Restenosis', 'Coronary Vessels', 'Disease Models, Animal', 'Drug-Eluting Stents', 'Follow-Up Studies', 'Paclitaxel', 'Probability', 'Random Allocation', 'Risk Factors', 'Survival Rate', 'Swine', 'Swine, Miniature', 'Time Factors', 'Treatment Outcome']
| 18,087,665
|
[['E02.148.050.060.100', 'E04.100.376.719.100', 'E04.100.814.529.124.060.100', 'E04.100.814.529.968.050', 'E04.502.382.124.060.100', 'E04.502.382.968.050', 'E04.928.220.520.100', 'E05.157.016.060.100'], ['B01.050'], ['E01.370.350.130.625', 'E01.370.350.700.060.200', 'E01.370.370.050.200', 'E01.370.370.380.200'], ['C14.280.647.250', 'C14.907.585.250'], ['C14.280.647.250.285.200', 'C14.907.585.250.285.200'], ['A07.015.114.269', 'A07.015.908.194'], ['C22.232', 'E05.598.500', 'E05.599.395.080'], ['E07.695.750.500'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['D02.455.426.392.368.242.888.777', 'D02.455.849.291.850.777'], ['E05.318.740.600', 'G17.680', 'N05.715.360.750.625', 'N06.850.520.830.600'], ['E05.318.370.700', 'E05.581.500.805', 'N05.715.360.325.675', 'N06.850.520.445.700'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['E05.318.308.985.550.900', 'N01.224.935.698.826', 'N06.850.505.400.975.550.900', 'N06.850.520.308.985.550.900'], ['B01.050.150.900.649.313.500.880'], ['B01.050.150.900.649.313.500.880.399.800'], ['G01.910.857'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Diseases [C]', 'Anatomy [A]', 'Health Care [N]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
3D ultrasound tissue motion tracking using correlation search.
|
Several methods for ultrasound tissue motion tracking and blood velocity estimation have been proposed and clinically applied. While providing valuable information to the clinician that was not obtainable from B-mode anatomical imaging, these methods still suffer from various fundamental and practical limitations that compromise their performance in certain clinical situations. A significant limitation is the inability of most of these methods to estimate the complete 3D motion or velocity vectors. With the introduction of ultrasound volumetric imaging, the need for a method that is capable of obtaining the complete motion vector is even more pressing. In this paper, we investigate the implementation of a correlation search scheme to estimate the 3D motion vectors using successive volumetric ultrasound scans. We present tracking results for motion along different axes and discuss the advantages and limitations of performing the correlation search in 3D.
|
['Algorithms', 'Humans', 'Image Processing, Computer-Assisted', 'Movement', 'Transducers', 'Ultrasonography, Doppler']
| 9,921,616
|
[['G17.035', 'L01.224.050'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['L01.224.308'], ['G07.568', 'G11.427.410'], ['E07.305.812'], ['E01.370.350.850.850']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Assessment of metal enrichment and their bioavailability in sediment and bioaccumulation by mangrove plant pneumatophores in a tropical (Zuari) estuary, west coast of India.
|
Sediment collected from the estuarine mangroves of the Zuari estuary and Cumbharjua canal were analyzed to assess the concentration, contamination and bioavailability of metals. Mangrove pneumatophores were also analyzed to understand the metal bioaccumulation in mangrove plants. The results indicated the variation of metal concentrations in sediment along the estuary was attributed to changing hydrodynamic conditions, type of sediment and metal sources. Further, speciation studies revealed that Fe, Cr, Co, Ni, Cu and Zn were mainly of lithogenic origin and less bioavailable while high Mn content in the sediment raised concerns over its potential mobility, bioavailability and subsequent toxicity. The mangrove plants exhibited difference in metal accumulation due to variations in sediment parameters and metal availability, in addition to difference in plant species and tissue physiology that affect metal uptake. Moreover, the mangrove plants reflected the quality of the underlying sediment and can be used as a potential bio-indicator tool.
|
['Biological Availability', 'Ecotoxicology', 'Environmental Monitoring', 'Estuaries', 'Geologic Sediments', 'India', 'Metals', 'Water Pollutants, Chemical', 'Wetlands']
| 27,325,605
|
[['G03.787.151', 'G07.690.725.129'], ['H01.158.273.248.500', 'H01.158.891.211', 'H01.277.249.500', 'H02.884.211'], ['N06.850.460.350.080', 'N06.850.780.375'], ['G01.311.625.540'], ['G01.311.330', 'G16.500.320'], ['Z01.252.245.393'], ['D01.552'], ['D27.888.284.903.655'], ['G16.500.275.157.812', 'N06.230.124.625']]
|
['Phenomena and Processes [G]', 'Disciplines and Occupations [H]', 'Health Care [N]', 'Geographicals [Z]', 'Chemicals and Drugs [D]']
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 1
|
[Retrospective study of 200 cases of nasopharyngeal carcinoma].
|
Nasopharyngeal carcinoma is frequently diagnosed in advanced stages due to the anatomic characteristics of its site of origin and of the inspecificity of many of its clinical manifestations. However, cure rates are directly related with the early diagnosis. In this study of 200 patients affected by this tumor we analyse the different clinical manifestations, their chronology and their value as early indicators of nasopharyngeal tumour.
|
['Carcinoma, Squamous Cell', 'Diagnosis, Differential', 'Humans', 'Nasopharyngeal Neoplasms', 'Otitis Media with Effusion', 'Recurrence', 'Retrospective Studies']
| 12,784,563
|
[['C04.557.470.200.400', 'C04.557.470.700.400'], ['E01.171'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C04.588.443.665.710.650', 'C07.550.350.650', 'C07.550.745.650', 'C09.647.710.650', 'C09.775.350.650', 'C09.775.549.650'], ['C09.218.705.663.670'], ['C23.550.291.937'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825']]
|
['Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Health Care [N]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Proximal muscle weakness in children.
|
In as many as half of the children referred to a muscle disease clinic, the original diagnosis is ultimately changed. Five cases histories are reviewed here in which Duchenne muscular dystrophy had been mistakenly diagnosed 3 to 12 years previously and the correct diagnosis was made only after repeat muscle biopsy or biopsy review. Four boys had diseases with a much better prognosis, and one boy did not have a muscle disease at all.
|
['Biopsy', 'Child', 'Child, Preschool', 'Diagnosis, Differential', 'Diagnostic Errors', 'Humans', 'Infant', 'Infant, Newborn', 'Male', 'Muscles', 'Muscular Dystrophies', 'Neuromuscular Diseases']
| 7,454,645
|
[['E01.370.225.500.384.100', 'E01.370.225.998.054', 'E01.370.388.100', 'E04.074', 'E05.200.500.384.100', 'E05.200.998.054', 'E05.242.384.100'], ['M01.060.406'], ['M01.060.406.448'], ['E01.171'], ['E01.354', 'N02.421.450.280'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703'], ['M01.060.703.520'], ['A02.633', 'A10.690'], ['C05.651.534.500', 'C10.668.491.175.500', 'C16.320.577'], ['C10.668']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Named Groups [M]', 'Health Care [N]', 'Organisms [B]', 'Anatomy [A]', 'Diseases [C]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
tPA promotes the proliferation of lung fibroblasts and activates the Wnt/â-catenin signaling pathway in idiopathic pulmonary fibrosis.
|
Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, irreversible and the most common fatal interstitial lung disease, which is characterized by damaged alveolar structure, the massive proliferation of fibroblasts and deposition of extracellular matrix (ECM). While the pathogenesis of IPF remains unclear, it has been clearly established that the excessive proliferation of lung fibroblasts is the most direct cause of fibrogenesis. Numerous proliferating fibroblasts form fibrous foci and secrete a large amount of ECM to aggravate the process of pulmonary fibrosis. Tissue plasminogen activator (tPA) is a kind of serine protease, its main function is to activate zymogens into active enzymes involved in fibrinolysis. Our study found tPA functioned as a cytokine to promote the proliferation of lung fibroblasts through intracellular signaling events involving Erk1/2, p90RSK, GSK-3â phosphorylation, and cyclinD1 induction. We also uncovered that tPA indirectly activated the Wnt/â-catenin signaling pathway by regulating the GSK-3â phosphorylation level. It's well-known that Wnt/â-catenin signaling pathway plays an important role in the pathogenesis of pulmonary fibrosis, in which the accumulation of â-catenin in the cytoplasm is an important signal of the activation of Wnt/â-catenin signaling pathway. Our study unveiled that tPA can serve as a cytokine involved in Wnt/â-catenin signaling pathway and be implicated in pulmonary fibrosis.
|
['Animals', 'Cell Proliferation', 'Cells, Cultured', 'Cyclin D1', 'Extracellular Matrix', 'Fibroblasts', 'Glycogen Synthase Kinase 3 beta', 'Idiopathic Pulmonary Fibrosis', 'Mice', 'Mice, Inbred C57BL', 'Mitogen-Activated Protein Kinase 1', 'Mitogen-Activated Protein Kinase 3', 'Ribosomal Protein S6 Kinases, 90-kDa', 'Tissue Plasminogen Activator', 'Wnt Signaling Pathway', 'beta Catenin']
| 31,550,972
|
[['B01.050'], ['G04.161.750', 'G07.345.249.410.750'], ['A11.251'], ['D12.644.360.262.150.100', 'D12.776.167.218.150.100', 'D12.776.476.262.150.100', 'D12.776.624.664.700.100'], ['A11.284.295.310'], ['A11.329.228'], ['D05.500.117.875.500', 'D08.811.913.696.620.682.700.429.500.500', 'D08.811.913.696.620.682.700.646.625.500', 'D12.644.360.300.500.500', 'D12.776.476.081.875.500', 'D12.776.476.300.500.500'], ['C08.381.765.500'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.420', 'B01.050.150.900.649.313.992.635.505.500.400.420'], ['D08.811.913.696.620.682.700.567.249.500', 'D12.644.360.450.169.500', 'D12.776.476.450.169.500'], ['D08.811.913.696.620.682.700.567.249.750', 'D12.644.360.450.169.750', 'D12.776.476.450.169.750'], ['D08.811.913.696.620.682.700.862.500', 'D12.644.360.600.500', 'D12.776.476.600.500'], ['D08.811.277.656.300.760.875', 'D08.811.277.656.959.350.875', 'D12.776.124.125.662.768', 'D23.119.970'], ['G02.111.820.925', 'G04.835.925'], ['D12.776.091.249', 'D12.776.220.145.500', 'D12.776.930.130']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Three-dimensional organization of the 30S ribosomal proteins from Escherichia coli. I. Preliminary classification of the proteins.
|
30S ribosomal subunits from Escherichia coli were reacted with three protein reagents. After reaction, the ribosomal proteins were extracted and examined; of the 20 proteins known to compose the particle, nine did not react with any of the three reagents. We have tentatively classified these proteins as "internal," and the remaining eleven as "external." A strong correlation was found between these results and the sequence of assembly. Those proteins which enter the assembly process early are classed as internal, whereas those proteins found to participate later in the assembly sequence are classed as external.
|
['Bacterial Proteins', 'Centrifugation, Density Gradient', 'Centrifugation, Zonal', 'Chromatography', 'Electrophoresis', 'Escherichia coli', 'Iodoacetates', 'Models, Biological', 'Nitrophenols', 'Ribosomes', 'Trypsin']
| 4,922,288
|
[['D12.776.097'], ['E05.181.724.336', 'E05.196.941.336'], ['E05.181.724.336.336', 'E05.196.941.336.419'], ['E05.196.181'], ['E05.196.401', 'E05.301.300'], ['B03.440.450.425.325.300', 'B03.660.250.150.180.100'], ['D02.241.081.018.487', 'D02.455.526.581.247'], ['E05.599.395'], ['D02.455.426.559.389.657.566', 'D02.640.743'], ['A11.284.430.214.190.875.811'], ['D08.811.277.656.300.760.895', 'D08.811.277.656.959.350.895']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Cesium and rubidium salts: effects on voluntary intake of ethanol by the rat.
|
The effects of RbCl and CsCl on voluntary intake of ethanol solution by rats preferring ethanol solution 5% (w/w) over water as the drinking fluid was studied as a function of the dose given and the vehicle injected. Administration of RbCl or CsCl, 0.5 mEq/kg/day or 1.5 mEq/kg/day for three consecutive days, did not alter amounts of ethanol consumed. Repeated administration of RbCl or CsCl, 3.0 mEq/kg/day for three days, produced some moderate reduction in ethanol consumption. Simultaneous injection of RbCl (1.5 mEq/kg) and CsCl (1.5 mEq/kg) resulted in greater and profound lasting decrease in ethanol drinking. The later treatment did not alter specific activities of rat liver alcohol- and aldehyde dehydrogenase from saline treated controls. In general, dissolving the chloride salt of the alkali metals in saline resulted in greater effects on ethanol drinking than that determined after identical dose injected with water as the vehicle. The possible mechanism(s) underlying the effects of alkali metal salts used are suggested.
|
['Alcohol Drinking', 'Animals', 'Cesium', 'Drinking Behavior', 'Feeding Behavior', 'Male', 'Rats', 'Rubidium']
| 733,853
|
[['F01.145.317.269'], ['B01.050'], ['D01.268.549.125', 'D01.268.556.165', 'D01.552.528.160', 'D01.552.544.165'], ['F01.145.317'], ['F01.145.113.547', 'F01.145.407', 'G07.203.650.353'], ['B01.050.150.900.649.313.992.635.505.700'], ['D01.268.549.650', 'D01.268.556.800', 'D01.552.528.759', 'D01.552.544.800']]
|
['Psychiatry and Psychology [F]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Outcomes for Correction of Long-Gap Esophageal Atresia: A 22-Year Experience.
|
BACKGROUND: Long-gap esophageal atresia (LGEA) precludes immediate primary repair. When delayed primary esophagoesophagostomy (DPE) is not feasible, a reverse gastric tube (RGT) is a potential salvage option. The purpose of this study was to determine if DPE and RGT had both similar short-term and long-term outcomes.METHODS: A retrospective review of all EA patients from 1994 to 2016 was undertaken. Data were stratified by surgical management (DPE versus RGT). Baseline demographics, operative information, postoperative management, and complications were analyzed. Descriptive statistics were used and P-values <0.05 were considered statistically significant.RESULTS: Two hundred and eighteen patients with EA were treated during this period; 37/218 (17%) had LGEA. Mean gap length was 3.3 ± 1.2 cm. Thirty-three patients underwent some form of repair, all of which were managed initially with a gastrostomy tube feeds. Twenty-five patients underwent DPE with 89% of these never requiring revision, and 86% having excellent function with long-term follow-up. In eight patients, esophageal length was never adequate for DPE; therefore, six were reconstructed with RGT, and two underwent gastric transposition. There were no significant differences in complications, revisions, ventilator days, overall length of stay, weight percentiles, or conduit function between children undergoing RGT compared with DPE at a mean follow-up of 5.5 years.CONCLUSIONS: Surgical treatment of LGEA is complex, and controversy exists regarding the optimal repair method when DPE is not feasible. In this series, DPE after gastrostomy tube feeds often allowed for sufficient esophageal lengthening with satisfactory long-term esophageal function. However, when adequate length for DPE was not attainable, these data suggest that RGT is a viable conduit with favorable postoperative outcomes.
|
['Esophageal Atresia', 'Esophagoplasty', 'Female', 'Humans', 'Infant', 'Male', 'Retrospective Studies']
| 32,113,037
|
[['C06.198.330', 'C06.405.117.260', 'C16.131.314.330'], ['E04.210.355'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825']]
|
['Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Named Groups [M]', 'Health Care [N]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Possible involvement of natural killer cells in bone marrow graft rejection.
|
In the present study comparison between Natural killer (NK) cells and cells responsible for rejection of bone marrow grafts was made. Both cell populations were found to be inhibited by cyclophosphamide, silica, carrageenan, and C. parvum. The reactivity of both cell populations occurred late in life and was not expressed in infant mice. Mice tolerant to bone marrow grafts and, therefore, accepting parental marrow transplants showed also decreased NK cell reactivities. These common features between NK cells and bone marrow effector cells suggest but not prove that these cells could represent the same cell population.
|
['Animals', 'Bacterial Vaccines', 'Bone Marrow Transplantation', 'Carrageenan', 'Cyclophosphamide', 'Female', 'Graft Rejection', 'Hematopoietic System', 'Killer Cells, Natural', 'Mice', 'Mice, Inbred Strains', 'Propionibacterium acnes', 'Silicon Dioxide', 'Transplantation, Homologous']
| 203,349
|
[['B01.050'], ['D20.215.894.135'], ['E02.095.147.725.040', 'E04.936.580.040'], ['D09.698.152'], ['D02.455.526.728.650.730.243', 'D02.705.672.500.243'], ['G12.875.545.328'], ['A15.378'], ['A11.118.637.555.567.537', 'A15.145.229.637.555.567.537', 'A15.382.490.555.567.537'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520', 'B01.050.150.900.649.313.992.635.505.500.400'], ['B03.510.024.990.600.600', 'B03.510.460.400.400.600.600.600'], ['D01.578.750', 'D01.650.550.825', 'D01.837.725'], ['E04.936.864']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
N-3 fatty acids modulate repeated stress-evoked pain chronicity.
|
N-3 fatty acids, including docosahexaenoic acid (DHA), have a beneficial effect in both pain and psychiatric disorders. In fact, we previously reported that stress-induced pain prolongation might be mediated through the suppression of the G-protein coupled-receptor 40/free fatty acid receptor 1 (GPR40/FFAR1), which is activated by DHA and long-chain fatty acids. However, the involvement of GPR40/FFAR1 ligands in the development of stress-induced chronic pain has not yet been described. In this study, we investigated the role of DHA in stress-evoked pain chronicity using diet-induced n-3 fatty acid deficient mice. The n-3 fatty acid deficient mice showed exacerbation of anxiety-like behavior after repeated exposure to social defeat stress. The intact n-3 fatty acid deficient mice showed a decrease in paw threshold values. On the other hand, paw withdrawal thresholds of defeated but not non-stressed, n-3 fatty acid deficient mice continued until day 49 after paw surgery. We evaluated changes in phosphatidylcholine composition in the brains of repeat stress-evoked chronic pain model mice which were not on n-3 fatty acid deficiency diets. On day 7 after paw surgery, phosphatidylcholines with DHA and other long-chain fatty acids were found to have decreased in the brains of stressed mice. Moreover, stress-induced persistent mechanical allodynia was improved by oral DHA supplementation. These results indicated that chronic stress may directly affect brain lipid composition; the related changes could be involved in chronic pain development. Our findings suggested that n-3 fatty acids, particularly DHA, are useful as a potential therapeutic target for stress-evoked chronic pain.
|
['Animals', 'Anxiety', 'Brain', 'Chronic Pain', 'Docosahexaenoic Acids', 'Fatty Acids', 'Fatty Acids, Omega-3', 'Female', 'Hyperalgesia', 'Male', 'Mice', 'Mice, Inbred C57BL', 'Mice, Inbred ICR', 'Receptors, G-Protein-Coupled', 'Signal Transduction', 'Stress, Psychological']
| 30,831,087
|
[['B01.050'], ['F01.470.132'], ['A08.186.211'], ['C23.888.592.612.274'], ['D10.212.302.380.410.210', 'D10.251.355.337.250', 'D10.627.430.450.375'], ['D10.251'], ['D10.212.302.380.410', 'D10.251.355.337', 'D10.627.430.450'], ['C10.597.751.791.400', 'C23.888.592.763.770.400'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.420', 'B01.050.150.900.649.313.992.635.505.500.400.420'], ['B01.050.050.199.520.520.510', 'B01.050.150.900.649.313.992.635.505.500.400.510'], ['D12.776.543.750.695'], ['G02.111.820', 'G04.835'], ['F01.145.126.990', 'F02.830.900']]
|
['Organisms [B]', 'Psychiatry and Psychology [F]', 'Anatomy [A]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Glycerol oxidation in rat brain: subcellular localization and kinetic characteristics.
|
The oxidation of [1,3-14C] glycerol to 14CO was measured in slices, whole homogenates, and subcellular fractions of rat brain. In all of these tissue preparations, the Lineweaver-Burk plots of glycerol oxidation were biphasic, yielding two apparent Km and V values. Similar kinetic characteristics were obtained with brain homogenates from guinea pig, mouse, rabbit, monkey, and pig. In other tissues of the rat, including heart, kidney, liver, and skeletal muscle, the Lineweaver-Burk plots for glycerol oxidation were not biphasic but were linear. Heating the brain homogenates for five minutes at 5 degrees C caused a 50% decrease in the rate of oxidation of glycerol without a change in the biphasic double reciprocal plot. The addition of purified glycerol kinase (EC 2.7.1.30) to the homogenate caused an increase in the rate of oxidation and resulted in linear Lineweaver-Burke plot. Brain mitochondria were prepared by two different methods, both of which yielded an enrichment of glycerol oxidation. In contrast, the rate of glucose oxidation was higher in homogenates than in mitochondria, and glucose competed with glycerol as substrate only extramitochondrially. The effects of various metabolic inhibitors suggested the participation of intact, coupled mitochondria, of glycolytic enzymes, and of electron transport in the oxidation of glycerol. The data support the primary localization of glycerol oxidation in nonsynaptic mitochondria in brain and the presence in that organelle of enzymes of the Embden-Meyerhoff pathway or an as yet unidentified system for oxidizing this compound.
|
['Animals', 'Brain', 'Carbon Dioxide', 'Glucose', 'Glycerol', 'Glycerol Kinase', 'Guinea Pigs', 'Haplorhini', 'Kinetics', 'Male', 'Mice', 'Mitochondria', 'Oxidation-Reduction', 'Rabbits', 'Rats', 'Species Specificity', 'Subcellular Fractions', 'Swine']
| 6,770,101
|
[['B01.050'], ['A08.186.211'], ['D01.200.200', 'D01.362.150', 'D01.650.550.200'], ['D09.947.875.359.448'], ['D02.033.800.875.500', 'D09.853.875.500'], ['D08.811.913.696.620.275'], ['B01.050.150.900.649.313.992.550'], ['B01.050.150.900.649.313.988.400'], ['G01.374.661', 'G02.111.490'], ['B01.050.150.900.649.313.992.635.505.500'], ['A11.284.430.214.190.875.564', 'A11.284.835.626'], ['G02.700', 'G03.295.531'], ['B01.050.150.900.649.313.968.700'], ['B01.050.150.900.649.313.992.635.505.700'], ['G16.824'], ['A11.284.835'], ['B01.050.150.900.649.313.500.880']]
|
['Organisms [B]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Professional ballet dancers have a similar prevalence of articular cartilage defects compared to age- and sex-matched non-dancing athletes.
|
Ballet exposes the hip joint to repetitive loading in extreme ranges of movement and may predispose a dancer to pain and osteoarthritis (OA). The aims of this study were to compare the prevalence of cartilage defects in professional ballet dancers and athletes and to determine the relationship of clinical signs and symptoms. Forty-nine male and female, current and retired professional ballet dancers and 49 age- and sex-matched non-dancing athletes completed hip pain questionnaires, including the Copenhagen Hip and Groin Outcome Score (HAGOS), and underwent hip range of movement (ROM) testing and 3-Tesla magnetic resonance imaging to score cartilage defects (no defect, grade 1: focal partial defect and grade 2: diffuse or full thickness defect). Thirty (61 %) dancers and 27 (55 %) athletes had cartilage defects (p = 0.54). The frequency of grade 1 and 2 cartilage defects did not differ between dancers and athletes (p = 0.83). The frequency of cartilage defects was similar in male and female dancers (p = 0.34), and male and female athletes (p = 0.24). Cartilage defects were not related to history of hip pain (p = 0.34), HAGOS pain (p = 0.14), sports/rec (p = 0.15) scores or hip internal rotation ?20° (p > 0.01). Cartilage defects were related to age in male dancers (p = 0.002). Ballet dancers do not appear to be at a greater risk of cartilage injury compared to non-dancing athletes. Male dancers develop cartilage defects at an earlier age than athletes and female dancers. Cartilage defects were not related to clinical signs and symptoms; thus, prospective studies are required to determine which cartilage defects progress to symptomatic hip OA.
|
['Adult', 'Athletes', 'Cartilage, Articular', 'Dancing', 'Female', 'Hip Joint', 'Humans', 'Magnetic Resonance Imaging', 'Male', 'Middle Aged', 'Osteoarthritis, Hip', 'Prevalence', 'Range of Motion, Articular', 'Severity of Illness Index', 'Surveys and Questionnaires']
| 27,567,627
|
[['M01.060.116'], ['M01.072'], ['A02.165.407.150', 'A02.835.583.192'], ['I03.450.642.287'], ['A02.835.583.411'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.350.825.500'], ['M01.060.116.630'], ['C05.550.114.606.400', 'C05.799.613.400'], ['E05.318.308.985.525.750', 'N01.224.935.597.750', 'N06.850.505.400.975.525.750', 'N06.850.520.308.985.525.750'], ['E01.370.600.700', 'G11.427.760'], ['E05.318.308.980.438.475.456.500', 'N05.715.360.300.800.438.375.364.500', 'N06.850.520.308.980.438.475.364.500'], ['E05.318.308.980', 'N05.715.360.300.800', 'N06.850.520.308.980']]
|
['Named Groups [M]', 'Anatomy [A]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Health Care [N]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 0
|
Isolation and characterization of human monoclonal antibodies to digoxin.
|
Fab preparations of sheep polyclonal anti-digoxin Abs have proven useful for reversal of the toxic effects of digoxin overdoses in patients. Unfortunately, the use of foreign species proteins in humans is limited because of the potential for immunological responses that include hypersensitivity reactions and acute anaphylaxis. Immunization of recently developed transgenic mice, whose endogenous micro heavy and kappa light chain Ig genes are inactivated and which carry human Ig gene segments, with a digoxin-protein conjugate has enabled us to generate and isolate eight hybridoma cell lines secreting human sequence anti-digoxin mAbs. Six of the mAbs have been partially characterized and shown to have high specificity and low nanomolar affinities for digoxin. In addition, detailed competition binding studies performed with three of these mAbs have shown them to have distinct differences in their digoxin binding, and that all three structural moieties of the drug, the primary digitoxose sugar, steroid, and five-member unsaturated lactone ring, contribute to Ab recognition.
|
['Animals', 'Antibodies, Monoclonal', 'Antibody Affinity', 'Binding Sites, Antibody', 'Binding, Competitive', 'Digoxin', 'Haptens', 'Humans', 'Hybridomas', 'Immunoglobulin G', 'Immunoglobulin Variable Region', 'Injections, Intraperitoneal', 'Mice', 'Mice, Inbred C57BL', 'Mice, Inbred CBA', 'Mice, Transgenic', 'Species Specificity']
| 10,438,974
|
[['B01.050'], ['D12.776.124.486.485.114.224', 'D12.776.124.790.651.114.224', 'D12.776.377.715.548.114.224'], ['G12.040', 'G12.122.125'], ['G02.111.570.060.425.079', 'G02.111.570.120.408', 'G12.122.232', 'G12.125'], ['E05.196.080', 'G02.111.084', 'G02.111.570.120.309'], ['D04.210.500.155.580.130.500.436', 'D04.210.500.155.580.130.688', 'D09.408.180.261.436'], ['D23.050.550.480'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A11.251.353.485', 'A11.251.600.485'], ['D12.776.124.486.485.114.619.393', 'D12.776.124.790.651.114.619.393', 'D12.776.377.715.548.114.619.393'], ['D12.644.541.500.650.500', 'D12.776.124.486.485.680.650.500', 'D12.776.124.486.485.797', 'D12.776.124.790.651.680.650.500', 'D12.776.124.790.651.797', 'D12.776.377.715.548.680.650.500', 'D12.776.377.715.548.797', 'G02.111.570.060.425'], ['E02.319.267.530.490'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.420', 'B01.050.150.900.649.313.992.635.505.500.400.420'], ['B01.050.050.199.520.520.440', 'B01.050.150.900.649.313.992.635.505.500.400.440'], ['B01.050.050.136.500', 'B01.050.150.900.649.313.992.635.505.500.800'], ['G16.824']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Early prednisone therapy in Henoch-Sch?nlein purpura: a randomized, double-blind, placebo-controlled trial.
|
OBJECTIVE: To evaluate the efficacy of early prednisone therapy in preventing renal and treating extrarenal and renal symptoms in Henoch-Sch?nlein purpura (HSP) in a placebo-controlled trial.STUDY DESIGN: A total of 171 patients (84 treated with prednisone and 87 receiving placebo) were included and followed up for 6 months. The endpoints were renal involvement at 1, 3, and 6 months and healing of extrarenal symptoms. The analyses were performed on an intent-to-treat basis.RESULTS: Prednisone (1 mg/kg/day for 2 weeks, with weaning over the subsequent 2 weeks) was effective in reducing the intensity of abdominal pain (pain score, 2.5 vs 4.8; P = .029) and joint pain (4.6 vs 7.3; P = .030). Prednisone did not prevent the development of renal symptoms but was effective in treating them; renal symptoms resolved in 61% of the prednisone patients after treatment, compared with 34% of the placebo patients (difference = 27%; 95% confidence interval = 3% to 47%; P = .024).CONCLUSIONS: The general use of prednisone in HSP is not supported, but patients with disturbing symptoms may benefit from early treatment, because prednisone reduces extrarenal symptoms and is effective in altering (but not preventing) the course of renal involvement.
|
['Abdominal Pain', 'Adolescent', 'Anti-Inflammatory Agents', 'Arthralgia', 'Child', 'Double-Blind Method', 'Drug Administration Schedule', 'Female', 'Humans', 'Kidney Diseases', 'Male', 'Prednisone', 'Prevalence', 'Prospective Studies', 'Purpura, Schoenlein-Henoch', 'Risk Factors', 'Severity of Illness Index', 'Treatment Outcome']
| 16,887,443
|
[['C23.888.592.612.054', 'C23.888.821.030'], ['M01.060.057'], ['D27.505.954.158'], ['C05.550.091', 'C23.888.592.612.094', 'F02.830.816.444.350', 'G11.561.790.444.350'], ['M01.060.406'], ['E05.318.370.300', 'E05.581.500.300', 'N05.715.360.325.320', 'N06.850.520.445.300'], ['E02.319.283'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C12.777.419', 'C13.351.968.419'], ['D04.210.500.745.432.719.702'], ['E05.318.308.985.525.750', 'N01.224.935.597.750', 'N06.850.505.400.975.525.750', 'N06.850.520.308.985.525.750'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['C14.907.940.777', 'C15.378.100.802.375', 'C15.378.463.515.580', 'C20.543.520.600', 'C23.550.414.950.375', 'C23.888.885.687.375'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['E05.318.308.980.438.475.456.500', 'N05.715.360.300.800.438.375.364.500', 'N06.850.520.308.980.438.475.364.500'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Diseases [C]', 'Named Groups [M]', 'Chemicals and Drugs [D]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Residual behavior and risk assessment of tridemorph in banana conditions.
|
An efficient method has been developed for determining tridemorph in banana using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The dissipation and terminal residue of tridemorph in banana fields were carried out at good agricultural practice (GAP) conditions. The average recoveries ranged from 84.4% to 90.0% with relative standard deviations (RSDs) of 3.0%-7.0% at three different spiking levels. The results indicated that the tridemorph dissipated quickly in banana with half-lives of 7.0-7.7days. The results of residual distribution ranged from 0.01 to 0.26mg/kg, 0.01-0.62mg/kg and <0.01mg/kg in whole banana, peel and pulp, respectively. The relationship between application factor and residue was discussed. The results of risk assessment showed that the risk quotient (RQ) value was all below RQ=1. Given that China has not set an maximum residue limit (MRL) value for tridemorph in banana, this study could provide guidance for the reasonable use of tridemorph.
|
['China', 'Chromatography, High Pressure Liquid', 'Food Analysis', 'Food Contamination', 'Half-Life', 'Morpholines', 'Musa', 'Pesticide Residues', 'Risk Assessment', 'Tandem Mass Spectrometry']
| 29,120,807
|
[['Z01.252.474.164'], ['E05.196.181.400.300'], ['E05.362', 'J01.576.423.850.100'], ['J01.576.423.850.730.500.249', 'N06.850.460.400', 'N06.850.601.500.249'], ['G01.910.405'], ['D03.383.533.640'], ['B01.650.940.800.575.912.250.618.937.555.500'], ['D27.720.031.700.672', 'D27.888.723.697', 'N06.850.460.200.700'], ['E05.318.740.600.800.715', 'N04.452.871.715', 'N05.715.360.750.625.700.690', 'N06.850.505.715', 'N06.850.520.830.600.800.715'], ['E05.196.566.880']]
|
['Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Technology, Industry, and Agriculture [J]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
|
Simultaneous modeling of the Kovats retention indices on OV-1 and SE-54 stationary phases using artificial neural networks.
|
In this study, a quantitative structure-property relationship technique has been used for the simultaneous prediction of Kovats retention indices for some esters, alcohols, aldehyde and ketones on OV-1 and SE-54 stationary phases, using an artificial neural network (ANN). The best-selected descriptors that appear in the models are the molecular values, number of atoms in each molecule, molecular shadow area on the xy plane and the energy level of the highest occupied molecular orbital. A 4-6-2 ANN was generated using these descriptors as inputs and its outputs will be the Kovats retention indices on OV-1 and SE-54 stationary phases. After optimization of the network parameters, the network was trained using a training set. For the evaluation of the predictive power of the generated ANN, an optimized network was used to predict the Kovats retention indices of the prediction set. The results obtained in this study showed that the average percentage deviation between the predicted ANN and the experimental values of Kovats retention indices for the prediction set were 2.5 and 3.0% on the OV-1 and SE-54 stationary phases, respectively. These values are in good agreement with the experimental results.
|
['Chromatography, Gas', 'Models, Chemical', 'Neural Networks, Computer']
| 12,075,931
|
[['E05.196.181.349'], ['E05.599.495'], ['G17.485', 'L01.224.050.375.605']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Information Science [L]']
| 0
| 0
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
What do nurses do when they take to the streets? An analysis of psychiatric and mental health nursing interventions in the community.
|
Little research can be found on nursing practice for populations needing non-traditional mental health care. A descriptive study was done with the nurses on the Community Link Service (CLS), an intensive psychiatric community follow-up program, to identify nursing interventions and the types of situations encountered. These nurses use the guiding principles of two current treatment modalities, Assertive Community Treatment and Clinical Case Management. The Nursing Interventions Classification System (NIC, McCloskey & Bulecheck, 2000) was used to code nursing interventions reported through interviews and critical incidents. The nursing interventions represented 93 interventions and included 400 different activities. The analysis showed that Case Management (79%) and Complex Relationship Building (71%) were their most common interventions followed by Medication Management (64%) and Surveillance (60%). Systems such as the NIC are useful tools, but they may not give a complete description of nursing practice in this setting.
|
['Case Management', 'Community Health Nursing', 'Community Mental Health Services', 'Follow-Up Studies', 'Humans', 'Mental Disorders', 'Nurse-Patient Relations', "Practice Patterns, Physicians'", 'Psychiatric Nursing']
| 16,335,355
|
[['N04.590.233.624.250'], ['H02.478.676.150', 'N02.421.143.150'], ['F04.408.307', 'N02.421.143.183', 'N02.421.461.232'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F03'], ['F01.829.401.650.600', 'N05.300.660.560'], ['N04.590.374.577', 'N05.300.625'], ['H02.478.676.710', 'N02.421.533.778']]
|
['Health Care [N]', 'Disciplines and Occupations [H]', 'Psychiatry and Psychology [F]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
|
Assessing continuing care needs: findings from the Fife Healthcare Assessment for Placement Project for elderly people.
|
Previous surveys of elderly patients in different continuing care settings suggest considerable overlap between settings. However, re-analysis of this data in terms of patients' profiles of dependency reveals a somewhat different picture, suggesting that overlap is not such a general phenomenon. Further data from new surveys in Fife confirm this view. The findings of these studies raise questions about some of the previous research on "misplacement' of people in long-stay care, and suggest that planners may need to be more discriminating in their analysis of which types of patient to transfer and what continuing care resources might be required. Failure to address these issues may lead to undue pressure on many of the continuing care placements.
|
['Activities of Daily Living', 'Aged', 'Geriatric Assessment', 'Health Services Needs and Demand', 'Health Services Research', 'Health Surveys', 'Housing for the Elderly', 'Humans', 'Patient Transfer', 'Scotland', 'Skilled Nursing Facilities']
| 8,655,302
|
[['E02.760.169.063.500.067', 'E02.831.067', 'I03.050', 'N02.421.784.110'], ['M01.060.116.100'], ['E05.318.308.225', 'I01.240.425.350', 'N01.224.425.350', 'N05.715.360.300.360', 'N06.850.505.400.425.350', 'N06.850.520.308.225'], ['N03.349.380.420', 'N05.300.450'], ['H01.770.644.145.360', 'N03.349.380', 'N05.425'], ['E05.318.308.980.438', 'N05.715.360.300.800.438', 'N06.850.520.308.980.438'], ['J03.340.260', 'N01.224.791.400.410', 'N06.230.150.360.260'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.760.169.624', 'E02.760.400.630', 'N02.421.585.169.624', 'N02.421.585.400.630', 'N04.590.233.727.210.624'], ['Z01.542.363.766'], ['N02.278.825.610.770']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]', 'Named Groups [M]', 'Disciplines and Occupations [H]', 'Technology, Industry, and Agriculture [J]', 'Organisms [B]', 'Geographicals [Z]']
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 1
| 0
| 1
| 1
| 1
|
The case for uncontrolled clinical trials: a starting point for the evidence base for CAM.
|
Clinical research into the effectiveness of any therapy usually progresses in a series of steps that are aimed at providing evidence that will lead to improved patient care. One important early step is the uncontrolled trial. Uncontrolled trials serve several crucial purposes, including establishing firmly that there is a clinical effect worth investigating, identifying the most suitable patients and the most appropriate treatments, and providing information on how large the effect might be. They can be conducted relatively easily by practitioners in the course of their normal work, and are an excellent first step for anyone interested in clinical research. Such studies are not only satisfying, but develop skills in searching the literature and writing protocols, and promote rigorous thinking and attention to detail. The ultimate purpose of uncontrolled trials is to facilitate subsequent definitive studies that will help define the place of CAM in health care.
|
['Clinical Trials as Topic', 'Complementary Therapies', 'Ethics, Medical', 'Evidence-Based Medicine', 'Humans', 'Sample Size']
| 11,444,891
|
[['E05.318.372.250.250', 'N05.715.360.330.250.250', 'N06.850.520.450.250.250'], ['E02.190'], ['K01.752.566.479.171.132.750', 'N05.350.340.162.500'], ['H02.249.750', 'H02.403.200.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.370.762', 'E05.581.500.902', 'N05.715.360.325.692', 'N06.850.520.445.762']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Humanities [K]', 'Disciplines and Occupations [H]', 'Organisms [B]']
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
|
The ability of bovine mammary tissue to synthesize lipids for 96 h when cocultured with liver and adipose tissue.
|
Explants of bovine mammary, liver, and adipose tissues were cocultured in the same well for 24, 48, 72 or 96 h and their ability to synthesize lipids was measured by the incorporation of [14C]acetate into triglycerides, free fatty acids, and phospholipids. The model was developed to study the effect of bovine somatotropin on lipid synthesis in mammary tissue, because somatotropin will not affect mammary tissue unless liver tissue is present. Somatotropin reduced incorporation by mammary tissue relative to the control. However, the rate of incorporation remained constant through 96 h with somatotropin, but steadily decreased in control mammary tissue. Phospholipid release into the media increased dramatically from 2% of that synthesized during the first 24 h to 218% of that synthesized from 72 to 96 h. The high release of phospholipids after 24 h suggests that cellular breakdown was occurring. A 24-h incubation is recommended to study lipid synthesis with this system.
|
['Adipose Tissue', 'Animals', 'Cattle', 'Female', 'Growth Hormone', 'Insulin-Like Growth Factor I', 'Lipids', 'Liver', 'Mammary Glands, Animal', 'Membrane Lipids', 'Organ Culture Techniques', 'Phospholipids', 'Temperature', 'Time Factors']
| 1,497,862
|
[['A10.165.114'], ['B01.050'], ['B01.050.150.900.649.313.500.380.271'], ['D06.472.699.631.525.425', 'D12.644.548.691.525.425'], ['D12.644.276.937.400', 'D12.776.124.862.400', 'D12.776.467.937.400', 'D23.529.937.400'], ['D10'], ['A03.620'], ['A10.336.482', 'A13.589'], ['D10.570'], ['E05.481.500.484'], ['D10.570.755'], ['G01.906.595', 'G16.500.275.063.725.710', 'G16.500.750.775.710', 'N06.230.150.450', 'N06.230.300.100.725.710'], ['G01.910.857']]
|
['Anatomy [A]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Pseudohalide anions reveal a novel extracellular site for potentiators to increase CFTR function.
|
BACKGROUND AND PURPOSE: There is great interest in the development of potentiator drugs to increase the activity of the cystic fibrosis transmembrane conductance regulator (CFTR) in cystic fibrosis. We tested the ability of several anions to potentiate CFTR activity by a novel mechanism.EXPERIMENTAL APPROACH: Patch clamp recordings were used to investigate the ability of extracellular pseudohalide anions (Co(CN)(6) (3-) , Co(NO(2) )(6) (3-) , Fe(CN)(6) (3-) , IrCl(6) (3-) , Fe(CN)(6) (4-) ) to increase the macroscopic conductance of mutant CFTR in intact cells via interactions with cytoplasmic blocking anions. Mutagenesis of CFTR was used to identify a possible molecular mechanism of action. Transepithelial short-circuit current recordings from human airway epithelial cells were used to determine effects on net anion secretion.KEY RESULTS: Extracellular pseudohalide anions were able to increase CFTR conductance in intact cells, as well as increase anion secretion in airway epithelial cells. This effect appears to reflect the interaction of these substances with a site on the extracellular face of the CFTR protein.CONCLUSIONS AND IMPLICATIONS: Our results identify pseudohalide anions as increasing CFTR function by a previously undescribed molecular mechanism that involves an interaction with an extracellular site on the CFTR protein. Future drugs could utilize this mechanism to increase CFTR activity in cystic fibrosis, possibly in conjunction with known intracellularly-active potentiators.
|
['Animals', 'Cell Line', 'Cobalt', 'Cricetinae', 'Cyanides', 'Cystic Fibrosis Transmembrane Conductance Regulator', 'Humans', 'Iridium', 'Mutation', 'Platinum Compounds']
| 22,612,315
|
[['B01.050'], ['A11.251.210'], ['D01.268.556.185', 'D01.268.956.155', 'D01.552.544.185'], ['B01.050.150.900.649.313.992.635.075.250'], ['D01.248.497.158.291', 'D01.625.400.100'], ['D12.776.157.530.100.304.500', 'D12.776.157.530.400.175.125', 'D12.776.157.530.450.074.500.500.500.500', 'D12.776.543.550.450.175.125', 'D12.776.543.585.100.304.500', 'D12.776.543.585.400.175.125', 'D12.776.543.585.450.074.500.500.500.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D01.268.556.401', 'D01.268.956.280', 'D01.552.544.401'], ['G05.365.590'], ['D01.710']]
|
['Organisms [B]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Bimatoprost versus Mometasone Furoate in the Treatment of Scalp Alopecia Areata: A Pilot Study.
|
BACKGROUND: Alopecia areata (AA) is an immune-mediated disease that targets anagen hair follicles. Despite various therapeutic options, there is no cure for AA. Prostaglandin analogues have been recognized as being capable of inducing hypertrichosis.OBJECTIVE: To compare the efficacy and safety of bimatoprost to those of corticosteroid in the treatment of scalp AA.METHODS: Thirty adult patients with patchy AA (S1) were included. Two AA patches were randomly assigned to treatment either by mometasone furoate 0.1% cream once daily (area A) or bimatoprost 0.03% solution twice daily (area B) for 3 months. Patients were assessed using the Severity of Alopecia Tool (SALT) scoring system for hair re-growth.RESULTS: All responding AA patches showed significant reduction in their SALT score after therapy. Area B demonstrated significantly better results regarding rapidity of response in weeks, percentage of hair re-growth and side effects compared to area A.CONCLUSION: Bimatoprost solution represents a therapeutic option for scalp AA.
|
['Administration, Cutaneous', 'Adult', 'Alopecia Areata', 'Bimatoprost', 'Dermatologic Agents', 'Female', 'Hair', 'Humans', 'Male', 'Middle Aged', 'Mometasone Furoate', 'Pilot Projects', 'Prospective Studies', 'Scalp', 'Severity of Illness Index', 'Single-Blind Method', 'Skin Cream', 'Young Adult']
| 25,765,294
|
[['E02.319.267.120.060'], ['M01.060.116'], ['C17.800.329.937.122.147'], ['D02.065.141', 'D10.251.355.255.550.775.500.175.250', 'D23.469.050.175.725.775.500.175.250', 'D23.469.700.670.175.250'], ['D27.505.954.444'], ['A17.360'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['D04.210.500.745.432.719.526'], ['E05.318.372.750', 'E05.337.737', 'N05.715.360.330.720', 'N06.850.520.450.720'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['A01.456.810'], ['E05.318.308.980.438.475.456.500', 'N05.715.360.300.800.438.375.364.500', 'N06.850.520.308.980.438.475.364.500'], ['E05.318.370.850', 'N05.715.360.325.730', 'N06.850.520.445.850'], ['E02.547.800.500', 'J01.516.213.249'], ['M01.060.116.815']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Named Groups [M]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Organisms [B]', 'Health Care [N]', 'Technology, Industry, and Agriculture [J]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 1
| 1
| 0
|
RhoA-sensitive trafficking of muscarinic acetylcholine receptors.
|
The clathrin-mediated sequestration pathway is used by non-G protein-coupled receptors (e.g., transferrin receptors) and a large number of G protein-coupled receptors, including beta-2 adrenoceptors and various muscarinic acetylcholine receptor (mAChR) subtypes. Recently, the ubiquitously expressed small GTPase RhoA has been implicated as a negative regulator of transferrin receptor internalization. Because mAChRs and other G protein-coupled receptors are able to activate RhoA, we investigated in HEK-293 cells whether RhoA regulates the sequestration of m1 and m2 mAChRs, which internalize via clathrin-coated and nonclathrin-coated vesicles in HEK-293 cells, respectively. Overexpression of wild-type RhoA inhibited agonist-induced sequestration of both m1 and m2 mAChRs by as much as 70%. Inhibition could be reversed by coexpression of Clostridium botulinum C3 transferase, which inactivates RhoA by ADP-ribosylation. Overexpression of C3 transferase alone had no effect on m1 and m2 mAChR sequestration. In addition, overexpression of RhoA inhibited m1 and m2 mAChR transport to the plasma membrane by 60 and 31%, respectively, which was blocked by coexpression of C3 transferase. We conclude that RhoA is not an endogenous regulator of mAChR sequestration, but when overexpressed, strongly inhibits mAChR trafficking (i.e., sequestration and transport to the plasma membrane) in HEK-293 cells.
|
['Biological Transport', 'Cell Line, Transformed', 'Clathrin', 'GTP-Binding Proteins', 'Humans', 'Receptor, Muscarinic M1', 'Receptor, Muscarinic M2', 'Receptors, Cell Surface', 'Receptors, Muscarinic', 'rhoA GTP-Binding Protein']
| 9,862,750
|
[['G03.143'], ['A11.251.210.172'], ['D12.776.543.990.200'], ['D08.811.277.040.330.300', 'D12.776.157.325'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.776.543.750.695.475.100', 'D12.776.543.750.720.360.500.099'], ['D12.776.543.750.695.475.200', 'D12.776.543.750.720.360.500.200'], ['D12.776.543.750'], ['D12.776.543.750.695.475', 'D12.776.543.750.720.360.500'], ['D08.811.277.040.330.300.400.700.200', 'D12.644.360.525.700.200', 'D12.776.157.325.515.700.200', 'D12.776.476.525.700.200']]
|
['Phenomena and Processes [G]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Organisms [B]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Seasonal variation, weather and behavior in day-care children: a multilevel approach.
|
This study analyzes the effect of weather variables, such as solar radiation, indoor and outdoor air temperature, relative humidity and time spent outdoor, on the behavior of 2-year-old children and their affects across different seasons: winter, spring and summer. Participants were a group of 61 children (33 males and 28 females) attending four day-care centers in Florence (Central Italy). Mean age of children at the beginning of the study was 24.1 months (SD = 3.6). We used multilevel linear analyses to account for the hierarchical structure of our data. The study analyzed the following behavioral variables: Activity Level, Attentional Focusing, Frustration, and Aggression. Results showed a different impact of some weather variables on children's behavior across seasons, indicating that the weather variable that affects children's behavior is usually the one that shows extreme values during the studied seasons, such as air temperature and relative humidity in winter and summer. Studying children and their reactions to weather conditions could have potentially wide-reaching implications for parenting and teaching practices, as well as for researchers studying social relationships development.
|
['Aggression', 'Child Behavior', 'Child, Preschool', 'Day Care, Medical', 'Environment', 'Female', 'Frustration', 'Humans', 'Italy', 'Male', 'Seasons', 'Weather']
| 23,238,531
|
[['F01.145.126.125', 'F01.145.813.045'], ['F01.145.179'], ['M01.060.406.448'], ['E02.760.246', 'N02.421.585.246'], ['G16.500.275', 'N06.230'], ['F01.470.405'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['Z01.542.489'], ['G01.910.645.661', 'G16.500.275.071.590', 'N06.230.300.100.250.525'], ['G16.500.275.063.725', 'G16.500.750.775', 'N06.230.300.100.725']]
|
['Psychiatry and Psychology [F]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Geographicals [Z]']
| 0
| 1
| 0
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Pulmonary veno-occlusive disease: diagnostic and therapeutic alternatives.
|
Pulmonary veno-occlusive disease (PVOD) is a rare cause of pulmonary hypertension. Surgical biopsy was usually required for diagnostic confirmation. However, the morbidity, mortality and limited benefit of this procedure have generated discussion regarding noninvasive diagnostic techniques. We present the case of a female patient with progressive dyspnea, hypoxemia and pulmonary hypertension, the last diagnosed via catheterization. Computed tomography revealed septal thickening and diffuse micronodules. Bronchoalveolar lavage revealed occult alveolar hemorrhage. Treatment with an endothelin antagonist was started, resulting in symptomatic and functional improvement. Occult alveolar hemorrhage differentiates PVOD from idiopathic pulmonary hypertension. We believe that this finding, in combination with characteristic tomographic findings, is sufficient to establish a diagnosis of PVOD.
|
['Biopsy', 'Bronchoalveolar Lavage', 'Bronchoscopy', 'Endothelin Receptor Antagonists', 'Female', 'Humans', 'Hypertension, Pulmonary', 'Lung', 'Middle Aged', 'Pulmonary Veno-Occlusive Disease', 'Receptors, Endothelin']
| 18,982,212
|
[['E01.370.225.500.384.100', 'E01.370.225.998.054', 'E01.370.388.100', 'E04.074', 'E05.200.500.384.100', 'E05.200.998.054', 'E05.242.384.100'], ['E05.927.100'], ['E01.370.386.105', 'E01.370.388.250.100', 'E04.502.250.100', 'E04.928.600.080'], ['D27.505.519.364'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C08.381.423', 'C14.907.489.556'], ['A04.411'], ['M01.060.116.630'], ['C08.381.780', 'C14.907.690'], ['D12.776.543.750.695.220', 'D12.776.543.750.750.320']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Diseases [C]', 'Anatomy [A]', 'Named Groups [M]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Challenges to using an electronic personal health record by a low-income elderly population.
|
BACKGROUND: Electronic personal health records (PHRs) are increasingly recognized and used as a tool to address various challenges stemming from the scattered and incompatible personal health information that exists in the contemporary US health care system. Although activity around PHR development and deployment has increased in recent years, little has been reported regarding the use and utility of PHRs among low-income and/or elderly populations.OBJECTIVE: The aim was to assess the use and utility of PHRs in a low-income, elderly population.METHODS: We deployed a Web-based, institution-neutral PHR system, the Personal Health Information Management System (PHIMS), in a federally funded housing facility for low-income and elderly residents. We assessed use and user satisfaction through system logs, questionnaire surveys, and user group meetings.RESULTS: Over the 33-month study period, 70 residents participated; this number was reduced to 44 by the end of the study. Although the PHIMS was available for free and personal assistance and computers with Internet connection were provided without any cost to residents, only 13% (44/330) of the eligible residents used the system, and system usage was limited. Almost one half of the users (47%, 33/70) used the PHIMS only on a single day. Use was also highly correlated with the availability of in-person assistance; 77% of user activities occurred while the assistance was available. Residents' ability to use the PHR system was limited by poor computer and Internet skills, technophobia, low health literacy, and limited physical/cognitive abilities. Among the 44 PHIMS users, 14 (32%) responded to the questionnaire. In this selected subgroup of survey participants, the majority (82%, 9/11) used the PHIMS three times or more and reported that it improved the quality of overall health care they received.CONCLUSIONS: Our findings suggest that those who can benefit the most from a PHR system may be the least able to use it. Disparities in access to and use of computers, the Internet, and PHRs may exacerbate health care inequality in the future.
|
['Adult', 'Aged', 'Aged, 80 and over', 'Delivery of Health Care', 'Education, Nursing, Graduate', 'Electronic Health Records', 'Female', 'Health Services for the Aged', 'Humans', 'Internet', 'Male', 'Medical Records Systems, Computerized', 'Middle Aged', 'Patient Participation', 'Poverty', 'Young Adult']
| 19,861,298
|
[['M01.060.116'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['N04.590.374', 'N05.300'], ['I02.358.337.450', 'I02.358.462.565'], ['E05.318.308.940.968.625.500', 'N04.452.859.564.650.125', 'N05.715.360.300.715.500.530.250', 'N06.850.520.308.940.968.625.250'], ['N02.421.320'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['L01.224.230.110.500'], ['E05.318.308.940.968.625', 'L01.313.500.750.300.695', 'N04.452.859.564.650', 'N05.715.360.300.715.500.530', 'N06.850.520.308.940.968.625'], ['M01.060.116.630'], ['F01.100.150.750.500.620', 'F01.145.488.887.500.620', 'N02.421.143.212.300', 'N03.540.245.360.300', 'N05.300.150.800.500.620'], ['I01.880.735.634', 'I01.880.853.996.535', 'N01.824.600'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Health Care [N]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Information Science [L]', 'Psychiatry and Psychology [F]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 1
| 1
| 0
|
Interferon consensus sequence-binding protein is constitutively expressed and differentially regulated in the ocular lens.
|
Interferon signaling is mediated by STATs and interferon regulatory factor (IRF) families of transcription factors. Ten distinct IRFs have been described and most are expressed in a variety of cells except for interferon consensus sequence-binding protein (ICSBP) and lymphoid-specific IRF/Pip that are thought to be exclusively expressed in lymphoid cells. We show here for the first time that ICSBP is constitutively and inducibly expressed in the mouse lens. In contrast to lymphoid cells with exclusive expression of ICSBP in the nucleus, ICSBP is present in both the cytoplasm and nucleus of the lens cell. However, ICSBP in the nucleus is of lower apparent molecular weight. We further show that the ICSBP promoter is constitutively bound by lens nuclear factors and that its activation requires binding of additional factors including STAT1. Furthermore, transcriptional activation of ICSBP gene by interferon gamma is accompanied by selective nuclear localization of ICSBP in proliferating epithelial cells but not in the nuclei of nondividing cells in the lens fiber compartment. Constitutive and inducible expression of ICSBP in the ocular lens and differential regulation of its subcellular localization in the developing lens suggest that ICSBP may have nonimmunity related functions and that the commonly held view that it is lymphoid-specific be modified.
|
['Animals', 'Cells, Cultured', 'Consensus Sequence', 'DNA-Binding Proteins', 'Epithelial Cells', 'Gene Expression Regulation', 'Immunohistochemistry', 'Interferon Regulatory Factors', 'Interferon-gamma', 'Lens, Crystalline', 'Mice', 'Mice, Inbred BALB C', 'Repressor Proteins', 'STAT1 Transcription Factor', 'Trans-Activators', 'Transcription, Genetic']
| 10,092,656
|
[['B01.050'], ['A11.251'], ['G02.111.570.580.175'], ['D12.776.260'], ['A11.436'], ['G05.308'], ['E01.370.225.500.607.512', 'E01.370.225.750.551.512', 'E05.200.500.607.512', 'E05.200.750.551.512', 'E05.478.583', 'H01.158.100.656.234.512', 'H01.158.201.344.512', 'H01.158.201.486.512', 'H01.181.122.573.512', 'H01.181.122.605.512'], ['D12.644.360.024.302', 'D12.776.157.057.050', 'D12.776.260.504', 'D12.776.476.024.385', 'D12.776.930.332'], ['D12.644.276.374.440.893', 'D12.644.276.374.480.615.350', 'D12.776.467.374.440.893', 'D12.776.467.374.480.615.350', 'D23.529.374.440.893', 'D23.529.374.480.615.350'], ['A09.371.060.500'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.338', 'B01.050.150.900.649.313.992.635.505.500.400.338'], ['D12.776.260.703', 'D12.776.930.780'], ['D12.644.360.024.303.500.500', 'D12.644.360.024.342.100', 'D12.776.157.057.061.500.500', 'D12.776.157.057.186.100', 'D12.776.260.513.249.500', 'D12.776.476.024.386.500.500', 'D12.776.476.024.430.100', 'D12.776.930.354.249.500', 'D12.776.930.840.100'], ['D12.776.260.755', 'D12.776.930.900', 'D12.776.964.925.984'], ['G02.111.873', 'G05.297.700']]
|
['Organisms [B]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
[Addison's disease as a rare cause of chronically elevated liver enzymes].
|
Common causes of chronically elevated serum liver enzymes include fatty liver disease, chronic viral hepatitis, autoimmune hepatitis, or hereditary metabolic disorders. Adrenocortical insufficiency can also cause elevated liver enzymes. Since 1990 only 11 cases have been reported. We here report a 52-year-old man with elevated liver enzymes (1.5 x upper limit of normal) over the past 10 years. Furthermore, hyponatremia and hyperkalemia were noted. He complained of fatigue and low blood pressure over the past few years. At physical examination a dark complexion was noted. After ruling out chronic viral hepatitis, autoimmune disease, metabolic or hereditary disorders, rare causes of elevated liver enzymes were considered. The endocrinological work-up revealed Addison's disease as cause of serum electrolyte disturbance and elevated liver enzymes. The patient was successfully treated with hydrocortisol and fludrocortisol. After one week, liver enzymes, serum electrolytes and arterial blood pressure had normalized. In conclusion, for patients with constantly elevated liver enzymes also rare, extrahepatic diseases have to be considered. Addison's disease is a rare but fully reversible cause for elevated liver enzymes.
|
['Addison Disease', 'Fludrocortisone', 'Humans', 'Hydrocortisone', 'Liver', 'Liver Diseases', 'Male', 'Middle Aged', 'Rare Diseases', 'Treatment Outcome']
| 16,456,760
|
[['C19.053.500.263', 'C20.111.163'], ['D04.210.500.745.745.654.600.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D04.210.500.745.745.654.600', 'D06.472.040.585.353.476', 'D06.472.040.585.478.392'], ['A03.620'], ['C06.552'], ['M01.060.116.630'], ['C23.550.291.906'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Diseases [C]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Anatomy [A]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
[Lateral arm free flap transfer to cover traumatic hand injuries].
|
The advantages of free flaps over distant pedicled flaps in hand reconstruction are well documented. They often require only a single operation, do not depend on local conditions of the wound and permit early physiotherapy. The lateral arm free flap is essentially suitable for reconstruction of the hand. It is thin, potentially sensate, and gives nearly perfect skin color and texture match. An additional technical advantage is the possibility of elevating the lateral arm free flap with tourniquet control at the same surgical field as the recipient site. We describe 3 cases of traumatic damage to skin and soft tissue of the hand, in which reconstruction was by both elective and emergency lateral arm, free flap transfer. They demonstrate the merits of this method of reconstruction.
|
['Adult', 'Arm', 'Hand Injuries', 'Humans', 'Male', 'Surgical Flaps']
| 1,572,576
|
[['M01.060.116'], ['A01.378.800.075'], ['C26.448'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A10.850.710', 'E07.862.710']]
|
['Named Groups [M]', 'Anatomy [A]', 'Diseases [C]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
The novel acidophilic structure of the killer toxin from halotolerant yeast demonstrates remarkable folding similarity with a fungal killer toxin.
|
BACKGROUND: Several strains of yeasts and fungi produce proteinous substances, termed killer toxins, which kill sensitive strains. The SMK toxin, secreted by the halotolerant yeast Pichia farinosa KK1 strain, uniquely exhibits its maximum killer activity under conditions of acidic pH and high salt concentration. The toxin is composed of two distinct subunits, alpha and beta, which tightly interact with each other under acidic conditions. However, they are easily dissociated under neutral conditions and lose the killer activity. The three-dimensional structure of the SMK toxin will provide a better understanding of the mechanism of toxicity of this protein and the cause of its unique pH-dependent stability.RESULTS: Two crystal structures of the SMK toxin have been determined at 1.8 A resolution in different ionic strength conditions. The two subunits, alpha and beta, are jointly folded into an ellipsoidal, single domain structure belonging to the alpha/beta-sandwich family. The folding topology of the SMK toxin is essentially the same as that of the fungal killer toxin, KP4. This shared topology contains two left-handed split betaalphabeta motifs, which are rare in the other proteins. Many acidic residues are clustered at the bottom of the SMK toxin molecule. Some of the carboxyl sidechains interact with each other through hydrogen bonds. The ionic strength difference induces no evident structural change of the SMK toxin except that, in the high ionic strength crystal, a number of sulfate ions are electrostatically bound near the basic residues which are also locally distributed at the bottom of the toxin molecule.CONCLUSIONS: The two killer toxins, SMK and KP4, share a unique folding topology which contains a rare structural motif. This observation may suggest that these toxins are evolutionally and/or functionally related. The pH-dependent stability of the SMK toxin is a result of the intensive interactions between the carboxyl groups. This finding is important for protein engineering, for instance, towards stabilization of the toxin molecule in a broader pH range. The present crystallographic study revealed that the structure of the SMK toxin itself is hardly affected by the ionic strength, implying that a high salt concentration affects the sensitivity of the cell against the toxin.
|
['Amino Acid Sequence', 'Crystallization', 'Crystallography, X-Ray', 'Dimerization', 'Fungal Proteins', 'Hydrogen Bonding', 'Killer Factors, Yeast', 'Models, Molecular', 'Molecular Sequence Data', 'Mycotoxins', 'Pichia', 'Protein Folding', 'Protein Processing, Post-Translational', 'Protein Structure, Secondary', 'Protein Structure, Tertiary', 'Sequence Alignment', 'Viral Proteins']
| 9,016,714
|
[['G02.111.570.060', 'L01.453.245.667.060'], ['E05.196.300', 'G02.171'], ['E05.196.309.742.225'], ['G02.206', 'G03.230'], ['D12.776.354'], ['G02.282'], ['D12.776.354.374', 'D23.946.587.531'], ['E05.599.595'], ['L01.453.245.667'], ['D23.946.587'], ['B01.300.107.795.700', 'B01.300.930.600'], ['G01.154.651', 'G02.111.688'], ['G02.111.660.871.790.600', 'G02.111.691.600', 'G03.734.871.790.600', 'G05.308.670.600'], ['G02.111.570.820.709.600'], ['G02.111.570.820.709.610'], ['E05.393.751'], ['D12.776.964']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
A structure-based multiple sequence alignment of all class I aminoacyl-tRNA synthetases.
|
The superimposable dinucleotide fold domains of MetRS, GlnRS and TyrRS define structurally equivalent amino acids which have been used to constrain the sequence alignments of the 10 class I aminoacyl-tRNA synthetases (aaRS). The conservation of those residues which have been shown to be critical in some aaRS enables to predict their location and function in the other synthetases, particularly: i) a conserved negatively-charged residue which binds the alpha-amino group of the amino acid substrate; ii) conserved residues within the inserted domain bridging the two halves of the dinucleotide-binding fold; and iii) conserved residues in the second half of the fold which bind the amino acid and ATP substrate. The alignments also indicate that the class I synthetases may be partitioned into two subgroups: a) MetRS, IleRS, LeuRS, ValRS, CysRS and ArgRS; b) GlnRS, GluRS, TyrRS and TrpRS.
|
['Amino Acid Sequence', 'Amino Acyl-tRNA Synthetases', 'Escherichia coli', 'Methionine-tRNA Ligase', 'Models, Chemical', 'Molecular Sequence Data', 'Protein Conformation', 'Sequence Alignment', 'Sequence Homology, Amino Acid']
| 7,647,112
|
[['G02.111.570.060', 'L01.453.245.667.060'], ['D08.811.464.263.200'], ['B03.440.450.425.325.300', 'B03.660.250.150.180.100'], ['D08.811.464.263.200.600'], ['E05.599.495'], ['L01.453.245.667'], ['G02.111.570.820.709'], ['E05.393.751'], ['G02.111.810.200', 'G05.810.200']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Strategies for pharmacologic treatment of high functioning autism and Asperger syndrome.
|
The treatment of complex, polymorphous disorders like HFA/AS always brings a particular challenge to pharmacotherapy. Additionally, the specific characteristics presented by HFA/AS introduce unique complications to patient care and place unusual demands on a clinician's skill and experience. To provide safe and effective treatment, the clinician must understand the core features of the disorder and the manifestations of the condition in his or her patient. Furthermore, a thorough understanding of the family, school, and community resources and limitations is necessary. Once an assessment has been made, focusing on target symptoms provides a crucial framework for care. Knowing manifestations of symptoms and characterizing their distribution and behavior in that patient is most important. For patients with HFA/AS it is particularly essential to coordinate behavioral and pharmacologic objectives. The target symptoms should be tracked carefully and placed into a priority system that is based on the risks and disability they create for the patient. The skill of pharmacotherapy also means setting out realistic expectations, keeping track of the larger systems of care at school and home, and collaboration with parents and care providers. There is an expanding range and pace of biologic and intervention research into HFA/AS. The genetic work has produced exciting leads that are likely to be helpful to future generations [82-84], but the task of clinicians is to tend to today's patients. As we discover more about the complex neural circuitry subserving repetitive behaviors, reward systems, and social cognition, there are good reasons to believe our treatments will become more sophisticated and specific. Psychopharmacology is also moving to design medications that target more specific populations of receptor and brain functions. This is likely to produce medicines that have fewer side effects, are more effective, and are more symptom-specific. Pharmacotherapy is not the ultimate treatment for HFA/AS but it has a definite place. Medication can be a critical element in a comprehensive treatment plan. There is a wider range of medications with more specific biologic effects than ever before. For patients with HFA/AS these newer agents are safer and less disruptive. When paired with clinicians who are becoming more skilled at recognizing and managing symptoms, patients have a greater opportunity to reach their potential and lead pleasurable lives.
|
['Adolescent', 'Asperger Syndrome', 'Autistic Disorder', 'Child', 'Combined Modality Therapy', 'Female', 'Humans', 'Intelligence', 'Male', 'Patient Care Team', 'Psychotropic Drugs']
| 12,512,397
|
[['M01.060.057'], ['F03.625.164.113.250'], ['F03.625.164.113.500'], ['M01.060.406'], ['E02.186'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F01.752.543'], ['N04.590.715'], ['D27.505.954.427.700']]
|
['Named Groups [M]', 'Psychiatry and Psychology [F]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Health Care [N]', 'Chemicals and Drugs [D]']
| 0
| 1
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Safety, efficacy, and clinical applicability of pulmonary vein isolation with circular multi-electrode ablation systems: PVAC® vs. nMARQ™ for atrial fibrillation ablation.
|
AIMS: We compare our experience with available circular multi-electrode catheters for atrial fibrillation (AF) ablation: PVAC(®), a phased radiofrequency system, and nMARQ™, an irrigated tip-CARTO-based technology.METHODS AND RESULTS: Prospective observational study of 175 consecutive patients with follow-up duration of at least 5 months who underwent pulmonary vein isolation (PVI) for symptomatic AF using PVAC(®) (n = 93, age 61.4 ± 9.8 years; 60% male, 13% persistent AF) vs. nMARQ™ (n = 82, age 63.2 ± 10.6 years; 67% male, 24% persistent AF). Procedure and radiation times were 94 ± 27 and 33 ± 13 min for PVAC(®) and 81 ± 18 and 30 ± 8.5 for nMARQ™ (P = 0.0008 and P = 0.18), respectively. The number of applications and the total burning time (min) were 20 ± 7 and 19 ± 6.7 for PVAC(®) and 16 ± 5.6 and 11 ± 4 for nMARQ™ (P < 0.0001 for both), respectively. In two nMARQ™ patients with small atria and pulmonary veins (PVs) and in two PVAC(®) patients with large PVs, the procedure failed; switching to the alternative technology was successful. Acute success rate was 97% for PVAC(®) and 95% for nMARQ™. There was one tamponade in nMARQ™ group and non-significant different minor complications for both techniques. One-year freedom from AF was 79 and 80.7% with PVAC(®) vs. nMARQ™, after one PVI, and 88 vs. 87.7% after two PVIs.CONCLUSION: Both technologies have short procedure and fluoroscopy times, comparable complication rates, and comparable acute and 1-year success rates. The number of applications and total procedure and burning times were shorter with nMARQ™. nMARQ™ was more suitable for larger atria and PVs. Thus, a patient-based pre-ablation anatomy definition is probably warranted for appropriate selection of technology type.
|
['Aged', 'Atrial Fibrillation', 'Catheter Ablation', 'Electrodes, Implanted', 'Equipment Design', 'Equipment Failure Analysis', 'Female', 'Fluoroscopy', 'Follow-Up Studies', 'Heart Atria', 'Humans', 'Israel', 'Male', 'Middle Aged', 'Prospective Studies', 'Pulmonary Veins', 'Treatment Outcome']
| 26,589,623
|
[['M01.060.116.100'], ['C14.280.067.198', 'C23.550.073.198'], ['E02.808.750.500', 'E04.014.760.500'], ['E07.305.250.319', 'E07.695.202'], ['E05.320'], ['E05.325.192'], ['E01.370.350.700.225'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['A07.541.358'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['Z01.252.245.500.375'], ['M01.060.116.630'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['A07.015.908.713'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Named Groups [M]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Anatomy [A]', 'Organisms [B]', 'Geographicals [Z]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Role of pharmacological aids and social supports in smoking cessation associated with Quebec's 2000 Quit and Win campaign.
|
BACKGROUND: This evaluation of the 2000 Quit and Win campaign in the province of Quebec, Canada, assessed the use and effectiveness of pharmacological aids, social support, and support resources (clinic program, support groups, books, telephone support) among contest participants. The reach of the contest was 1.3% of adult smokers: 20,400 participants.METHODS: Six months after the contest ended, 3,033 randomly selected participants completed telephone interviews about their smoking status and their use of nonform aids, social support, support resources, and pharmacological aids during their cessation attempt. Those who were abstinent from smoking were then reinterviewed 6 months later, that is, 12 months after the contest.RESULTS: Cessation rates were 66% at contest end, 36% at 6 months, and 22% at 12 months. Heavier smokers were somewhat more likely to have quit. Overall, 41% of respondents used any form of aid (support resources and pharmacological aids) in the first 6 months; among these, 42% used bupropion and 38% used nicotine patches. Those using bupropion were more likely to successfully quit smoking. Successful quitters rated the social support received from their buddy as more useful than did relapsers, and social support was unrelated to the use of pharmacological aids.CONCLUSIONS: The results suggest that adequate investment in population-wide Quit and Win programs that provide a variety of appropriate aids to smokers, including social support and pharmacological products, can improve the reach of smokers.
|
['Adolescent', 'Adult', 'Drug Therapy', 'Female', 'Humans', 'Male', 'Middle Aged', 'Quebec', 'Smoking Cessation', 'Social Support']
| 15,066,370
|
[['M01.060.057'], ['M01.060.116'], ['E02.319'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['Z01.107.567.176.791'], ['F01.145.488.732'], ['I01.880.853.500.600']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Geographicals [Z]', 'Psychiatry and Psychology [F]', 'Anthropology, Education, Sociology, and Social Phenomena [I]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 1
|
Shoulder pain: pathogenesis, diagnosis and management.
|
Shoulder pain is a common musculoskeletal problem that can have a significant effect on people's activities of daily living and quality of life. Nurses have a central role in the assessment, management and follow up of patients undergoing conservative and surgical treatments. Understanding the often complex and chronic nature of shoulder pain will help healthcare professionals to provide holistic patient-centred care, considering the most suitable treatment options for the individual.
|
['Analgesics', 'Humans', 'Pain Management', 'Patient-Centered Care', 'Practice Guidelines as Topic', 'Shoulder Pain']
| 24,471,638
|
[['D27.505.696.663.850.014', 'D27.505.954.427.040'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.745', 'N04.590.607.500'], ['N04.590.233.727.407'], ['N04.761.700.350.650', 'N05.700.350.650'], ['C05.550.091.700', 'C23.888.592.612.094.700', 'F02.830.816.444.350.500', 'G11.561.790.444.350.500']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Biochemical characteristics of AtFAR2, a fatty acid reductase from Arabidopsis thaliana that reduces fatty acyl-CoA and -ACP substrates into fatty alcohols.
|
Fatty alcohols and derivatives are important for proper deposition of a functional pollen wall. Mutations in specific genes encoding fatty acid reductases (FAR) responsible for fatty alcohol production cause abnormal development of pollen. A disrupted AtFAR2 (MS2) gene in Arabidopsis thaliana results in pollen developing an abnormal exine layer and a reduced fertility phenotype. AtFAR2 has been shown to be targeted to chloroplasts and in a purified form to be specific for acyl-ACP substrates. Here, we present data on the in vitro and in planta characterizations of AtFAR2 from A. thaliana and show that this enzyme has the ability to use both, C16:0-ACP and C16:0-CoA, as substrates to produce C16:0-alcohol. Our results further show that AtFAR2 is highly similar in properties and substrate specificity to AtFAR6 for which in vitro data has been published, and which is also a chloroplast localized enzyme. This suggests that although AtFAR2 is the major enzyme responsible for exine layer functionality, AtFAR6 might provide functional redundancy to AtFAR2.
|
['Acyl Carrier Protein', 'Acyl Coenzyme A', 'Aldehyde Oxidoreductases', 'Arabidopsis', 'Arabidopsis Proteins', 'Fatty Alcohols', 'Hydrogen-Ion Concentration', 'Kinetics', 'Plant Leaves', 'Serum Albumin, Bovine', 'Substrate Specificity', 'Tobacco']
| 27,274,541
|
[['D12.776.157.050'], ['D03.633.100.759.646.138.382.300', 'D08.211.211.300', 'D13.695.667.138.382.300', 'D13.695.827.068.382.300'], ['D08.811.682.657.163'], ['B01.650.940.800.575.912.250.157.100'], ['D12.776.765.149'], ['D02.033.415', 'D10.289'], ['G02.300'], ['G01.374.661', 'G02.111.490'], ['A18.024.812'], ['D12.776.034.841.540', 'D12.776.124.727.875'], ['G02.111.835'], ['B01.650.940.800.575.912.250.908.500.900']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Nucleotide and nucleic acid metabolism in rat thymocytes during cell cycle progression.
|
A complete cell cycle of mature, concanavalin A (Con A) stimulated rat thymocytes was documented by analyzing the cell number as well as the content and synthesis of DNA and RNA. Cell cycle progression is accompanied by an elevation of class I, II and III RNA polymerase activities (about 10-fold) in the S phase maximum, 48 h after stimulation. Moreover, maximal cellular contents of DNA, ATP, ADP and AMP were observed at this culture period, whereas the RNA level peaked at 60 h. The synthesis of purine and pyrimidine nucleotides de novo was detected by use of [14C]HCO3-. Maximal incorporation rates of [14C]HCO3- into nucleotides (de novo synthesis) and of [3H]adenine into adenylates ('salvage pathway') occur during the S phase. However, the de novo synthesis rates were markedly lower than those of the 'salvage pathway'. The highest cellular level of the nucleotide precursor 5-phosphoribosyl-1-pyrophosphate (8.4-fold increase) also coincided with the S phase.
|
['Animals', 'Cell Cycle', 'Concanavalin A', 'DNA', 'DNA-Directed RNA Polymerases', 'Female', 'Kinetics', 'Lymphocyte Activation', 'Nucleotides', 'Phosphoribosyl Pyrophosphate', 'RNA', 'Rats', 'T-Lymphocytes', 'Thymus Gland']
| 1,718,437
|
[['B01.050'], ['G04.144'], ['D12.776.503.499.500', 'D12.776.765.678.500'], ['D13.444.308'], ['D08.811.913.696.445.735.270'], ['G01.374.661', 'G02.111.490'], ['E01.370.225.812.482', 'E05.200.812.482', 'E05.478.594.530', 'G12.450.050.400.545', 'G12.565'], ['D09.408.620', 'D13.695'], ['D09.894.643.650'], ['D13.444.735'], ['B01.050.150.900.649.313.992.635.505.700'], ['A11.118.637.555.567.569', 'A15.145.229.637.555.567.569', 'A15.382.490.555.567.569'], ['A10.549.750', 'A15.382.520.604.750']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
To swim or not to swim: A population-level model of Xenopus tadpole decision making and locomotor behaviour.
|
We present a detailed computational model of interacting neuronal populations that mimic the hatchling Xenopus tadpole nervous system. The model includes four sensory pathways, integrators of sensory information, and a central pattern generator (CPG) network. Sensory pathways of different modalities receive inputs from an "environment"; these inputs are then processed and integrated to select the most appropriate locomotor action. The CPG populations execute the selected action, generating output in motor neuron populations. Thus, the model describes a detailed and biologically plausible chain of information processing from external signals to sensors, sensory pathways, integration and decision-making, action selection and execution and finally, generation of appropriate motor activity and behaviour. We show how the model produces appropriate behaviours in response to a selected scenario, which consists of a sequence of "environmental" signals. These behaviours might be relatively complex due to noisy sensory pathways and the possibility of spontaneous actions.
|
['Animals', 'Behavior, Animal', 'Decision Making', 'Larva', 'Locomotion', 'Models, Neurological', 'Motor Neurons', 'Nerve Net', 'Neural Inhibition', 'Swimming', 'Xenopus']
| 28,720,508
|
[['B01.050'], ['F01.145.113'], ['F02.463.785.373'], ['B05.500.500', 'G07.345.500.550.500.500'], ['G07.568.500', 'G11.427.410.568'], ['E05.599.395.642'], ['A08.675.655.500', 'A11.671.655.500'], ['A08.511'], ['G07.265.755', 'G11.561.616'], ['G11.427.410.568.800', 'G11.427.410.698.277.875', 'I03.350.875', 'I03.450.642.845.945.500'], ['B01.050.150.900.090.180.610.500']]
|
['Organisms [B]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Anthropology, Education, Sociology, and Social Phenomena [I]']
| 1
| 1
| 0
| 0
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
|
[The current status of organ transplantation: the role of xenotransplantation?].
|
In the last few years, transplantation was an area of intense research activity. However, there is a worldwide shortage of donor organs for clinical transplantations. Currently, interest in xenotransplantation research is growing not only because of the increased demand for organs but also because of advances in molecular biology techniques that make possible the genetic or immunological manipulations of the animal donor rather than the human recipient. The better definitions of the mechanisms responsible for xenograft rejection should facilitate appropriate therapeutic strategies for long xenograft survival.
|
['Animals', 'Genetic Techniques', 'Graft Rejection', 'Graft Survival', 'Humans', 'Immunologic Techniques', 'Molecular Biology', 'Organ Transplantation', 'Research', 'Tissue Donors', 'Tissue and Organ Procurement', 'Transplantation Immunology', 'Transplantation, Heterologous']
| 9,564,229
|
[['B01.050'], ['E05.393'], ['G12.875.545.328'], ['G12.875.545.340'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.478'], ['H01.158.201.636', 'H01.158.273.343.595', 'H01.181.122.650'], ['E04.936.450'], ['H01.770.644'], ['M01.898'], ['N02.421.911'], ['G12.875'], ['E04.936.764']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Disciplines and Occupations [H]', 'Named Groups [M]', 'Health Care [N]']
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 1
| 1
| 0
|
Fractal scaling mechanisms in biomembranes. Oscillations in the lateral diffusion coefficient.
|
A modified model of the Cohen-Turnbull free volume theory for lateral transport processes in biomembranes is presented. The model which is based on renormalization group theoretical concepts incorporates fractal rather than Markovian diffusion kinetics. It predicts harmonic oscillations in the lateral diffusion coefficient around a dominant power-law trend and clarifies, in addition, recently observed deviations from the Cohen-Turnbull exponential law.
|
['Cell Membrane', 'Diffusion', 'Membrane Fluidity', 'Membrane Proteins', 'Models, Theoretical', 'Periodicity']
| 2,924,739
|
[['A11.284.149'], ['G01.202', 'G02.196'], ['G01.154.500', 'G02.111.550', 'G04.570'], ['D12.776.543'], ['E05.599'], ['G01.910.645', 'G07.180.562']]
|
['Anatomy [A]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
C-peptide and insulin secretion in diabetes mellitus treated with oral hypoglycaemic agents or diet alone. A 3 years epidemiological cohort study on the Island of Falster, Denmark.
|
In a 3 yr epidemiological cohort study of 273 diabetics treated with oral hypoglycaemic agents (OHA) and 60 diet-treated diabetics the predictive value of fasting plasma C-peptide levels was assessed with the attempt to discriminate between insulin dependence and non-insulin dependence. Serum insulin, blood glucose, haemoglobin AI, bicarbonate, urine for ketone bodies, height and weight were measured too. All but 8 OHA-treated patients (97%) had fasting C-peptide greater than 0.40 pmol/ml at both investigations. Six had C-peptide in the interval 0.21-0.40 pmol/ml at both investigations and 2 a C-peptide less than or equal to 0.20 pmol/ml all of which became insulin dependent during the 3 yr period. The highest fasting C-peptide concentrations were found in overweight diabetics with a blood glucose level greater than 8.5 mmol/l. Overweight diabetics had significantly elevated fasting insulin compared to the normal weights but when the IRI concentrations were corrected by the body mass index hyperinsulinaemia was positively correlated with high levels of blood glucose and haemoglobin AI, i.e. poor glycaemic control and not with overweight. The results suggest that determination of fasting plasma C-peptide can be an additional clinical help in discriminating between insulin dependence and non-dependence.
|
['Adult', 'Blood Glucose', 'Body Weight', 'C-Peptide', 'Diabetes Mellitus', 'Diet, Diabetic', 'Female', 'Humans', 'Hypoglycemic Agents', 'Insulin', 'Insulin Secretion', 'Male', 'Middle Aged']
| 3,301,161
|
[['M01.060.116'], ['D09.947.875.359.448.500'], ['C23.888.144', 'E01.370.600.115.100.160.120', 'E05.041.124.160.750', 'G07.100.100.160.120', 'G07.345.249.314.120'], ['D06.472.699.587.200.500.250', 'D12.644.548.586.200.500.250'], ['C18.452.394.750', 'C19.246'], ['E02.642.249.240', 'G07.203.650.240.240'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D27.505.696.422'], ['D06.472.699.587.200.500.625', 'D12.644.548.586.200.500.625'], ['G03.442', 'G07.475'], ['M01.060.116.630']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
The Fate of Spacers in the Treatment of Periprosthetic Joint Infection.
|
BACKGROUND: Two-stage exchange arthroplasty remains the preferred method to treat periprosthetic joint infection. The aim of this study was to investigate the clinical course of periprosthetic joint infection following resection arthroplasty and insertion of a spacer.METHODS: Our institutional database was used to identify 504 cases of periprosthetic joint infection (326 knees and 178 hips) treated with resection arthroplasty and spacer insertion as part of a two-stage exchange arthroplasty. A review of the patient charts was performed to extract information relevant to the objectives of this study that included the details of the clinical course following resection arthroplasty.RESULTS: The mean follow-up duration after initial spacer implantation was 56.2 months. Reimplantation occurred in the joints of 417 (82.7%) of 504 cases. Of these 417 cases, 329 (78.9%) had a minimum one-year follow-up, and 81.4% of these had successful treatment. The mean duration from resection arthroplasty to reimplantation was 4.2 months (range, 0.7 to 131.7 months). Sixty (11.9%) of the 504 joints required interim spacer exchange(s). Of the eighty-seven cases that did not undergo reimplantation, six (6.9%) required amputation, five (5.7%) underwent a Girdlestone procedure, four (4.6%) underwent arthrodesis, and seventy-two (82.8%) underwent spacer retention. Thirty-six patients died in the interstage period.CONCLUSIONS: The commonly held belief that two-stage exchange arthroplasty carries a high success rate for the eradication of periprosthetic joint infection may need to be reexamined. A considerable number of patients undergoing the first stage of a two-stage procedure do not undergo a subsequent reimplantation for a variety of reasons or require an additional spacer exchange in the interim. Reports on the success of two-stage exchange should account for the mortality of these patients and for patients who never undergo reimplantation.
|
['Aged', 'Arthrodesis', 'Arthroplasty, Replacement, Hip', 'Arthroplasty, Replacement, Knee', 'Confidence Intervals', 'Databases, Factual', 'Device Removal', 'Female', 'Follow-Up Studies', 'Humans', 'Kaplan-Meier Estimate', 'Logistic Models', 'Male', 'Middle Aged', 'Prosthesis-Related Infections', 'Reoperation', 'Retrospective Studies', 'Risk Assessment', 'Severity of Illness Index', 'Statistics, Nonparametric', 'Survival Rate', 'Suture Anchors', 'Time Factors', 'Treatment Outcome']
| 26,378,265
|
[['M01.060.116.100'], ['E04.555.100'], ['E04.555.110.110.110', 'E04.650.110.110', 'E04.680.101.110.110'], ['E04.555.110.110.115', 'E04.650.110.115', 'E04.680.101.110.115'], ['E05.318.740.275', 'N05.715.360.750.220', 'N06.850.520.830.275'], ['L01.313.500.750.300.188.400', 'L01.470.750.750'], ['E04.199'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.740.998.650', 'N05.715.360.750.795.650', 'N06.850.520.830.998.650'], ['E05.318.740.500.525', 'E05.318.740.600.800.450', 'E05.318.740.750.450', 'E05.599.835.875', 'N05.715.360.750.530.480', 'N05.715.360.750.625.700.450', 'N05.715.360.750.695.470', 'N06.850.520.830.500.525', 'N06.850.520.830.600.800.450', 'N06.850.520.830.750.450'], ['M01.060.116.630'], ['C01.685', 'C23.550.767.868'], ['E04.690'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E05.318.740.600.800.715', 'N04.452.871.715', 'N05.715.360.750.625.700.690', 'N06.850.505.715', 'N06.850.520.830.600.800.715'], ['E05.318.308.980.438.475.456.500', 'N05.715.360.300.800.438.375.364.500', 'N06.850.520.308.980.438.475.364.500'], ['E05.318.740.995', 'N05.715.360.750.760', 'N06.850.520.830.995'], ['E05.318.308.985.550.900', 'N01.224.935.698.826', 'N06.850.505.400.975.550.900', 'N06.850.520.308.985.550.900'], ['E07.695.370.734', 'E07.858.442.660.460.734', 'E07.858.690.725.460.734'], ['G01.910.857'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Information Science [L]', 'Organisms [B]', 'Diseases [C]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 1
| 1
| 0
|
The effects of grommet insertion on Eustachian tube function.
|
A prospective study on the effect of grommet insertion on the passive Eustachian tube opening pressure was undertaken in children's ears with otitis media with effusion. After an initial immediate improvement following aspiration of the middle ear no further change in Eustachian tube function occurred over 4 subsequent weeks. Grommet insertion probably has no effect on Eustachian tube function.
|
['Child', 'Child, Preschool', 'Eustachian Tube', 'Humans', 'Middle Ear Ventilation', 'Otitis Media with Effusion', 'Tympanic Membrane']
| 8,877,219
|
[['M01.060.406'], ['M01.060.406.448'], ['A09.246.397.369'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E04.579.592', 'E04.580.450.472'], ['C09.218.705.663.670'], ['A09.246.272.702']]
|
['Named Groups [M]', 'Anatomy [A]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Ultraviolet difference spectroscopy study of peanut lectin binding to Mono- and disaccharides.
|
Ultraviolet difference spectroscopy was used to study the interaction of peanut (Arachis hypogaea) lectin with complementary carbohydrates. A correlation was observed between variations of ultraviolet spectra during the binding of sugars to the lectin and the specificity and the strength of the binding. The association constant, free energy, enthalpy and entropy for peanut lectin-lactose interactions were calculated over the temperature range 10-30 degrees C. The binding constants for 10 mono- and disaccharides containing a D-galactopyranosyl or a D-talopyranosyl residue were calculated. Comparing their effectiveness to interact with peanut lectin, methyl alpha-D-galactopyranoside appeared to have a more marked affinity than lactose; D-galacturonic acid and methyl 7-deoxy-D-glycero-beta-D-galacto-heptopyranoside had no measurable affinity; the other sugars showed a lower affinity than lactose. The correlations between these differences and the conformations of the sugars obtained by X-ray analysis are discussed.
|
['Binding Sites', 'Chemical Phenomena', 'Chemistry', 'Disaccharides', 'Lactose', 'Lectins', 'Monosaccharides', 'Peanut Agglutinin', 'Spectrophotometry, Ultraviolet']
| 7,126,638
|
[['G02.111.570.120'], ['G02'], ['H01.181'], ['D09.698.629.305', 'D09.947.750'], ['D09.698.629.305.340', 'D09.947.750.340'], ['D12.776.503'], ['D09.947.875'], ['D12.776.503.499.625', 'D12.776.765.678.625'], ['E05.196.712.726.802', 'E05.196.867.826.802']]
|
['Phenomena and Processes [G]', 'Disciplines and Occupations [H]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
[Content of total immunoglobulin E in patients with acute appendicitis].
|
The authors investigated the content of total immunoglobulin E in 34 patients with different forms of acute appendicitis. Higher level of immunoglobulin E was revealed in 19 patients. All the patients had destructive forms of appendicitis, in the third of the patients they were complicated by the development of local peritonitis or by spread of the inflammation outside the appendix. The postoperative period in patients with the elevated level of immunoglobulin E was characterized by a higher incidence of purulent wound complications which can be accounted for by the initially more severe forms of lesions of the appendix.
|
['Acute Disease', 'Appendicitis', 'Humans', 'Immunoenzyme Techniques', 'Immunoglobulin E', 'Peritonitis', 'Postoperative Complications', 'Suppuration', 'Surgical Wound Infection']
| 11,496,488
|
[['C23.550.291.125'], ['C01.463.099', 'C06.405.205.099', 'C06.405.469.110.207'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.478.566.350', 'E05.478.583.400', 'E05.601.470.350'], ['D12.776.124.486.485.114.619.312', 'D12.776.124.790.651.114.619.312', 'D12.776.377.715.548.114.619.312'], ['C01.463.600', 'C06.844.640'], ['C23.550.767'], ['C01.830', 'C23.550.470.756'], ['C01.947.692', 'C23.550.767.925']]
|
['Diseases [C]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
High dietary cholesterol masks type 2 diabetes-induced osteopenia and changes in bone microstructure in rats.
|
Type 2 diabetes mellitus (T2DM) often occurs concurrently with high blood cholesterol or dyslipidemia. Although T2DM has been hypothesized to impair bone microstructure, several investigations showed that, when compared to age-matched healthy individuals, T2DM patients had normal or relatively high bone mineral density (BMD). Since cholesterol and lipids profoundly affect the function of osteoblasts and osteoclasts, it might be cholesterol that obscured the changes in BMD and bone microstructure in T2DM. The present study, therefore, aimed to determine bone elongation, epiphyseal histology, and bone microstructure in non-obese T2DM Goto-Kakizaki rats treated with normal (GK-ND) and high cholesterol diet. We found that volumetric BMD was lower in GK-ND rats than the age-matched wild-type controls. In histomorphometric study of tibial metaphysis, T2DM evidently suppressed osteoblast function as indicated by decreases in osteoblast surface, mineral apposition rate, and bone formation rate in GK-ND rats. Meanwhile, the osteoclast surface and eroded surface were increased in GK-ND rats, thus suggesting an activation of bone resorption. T2DM also impaired bone elongation, presumably by retaining the chondrogenic precursor cells in the epiphyseal resting zone. Interestingly, several bone changes in GK rats (e.g., increased osteoclast surface) disappeared after high cholesterol treatment as compared to wild-type rats fed high cholesterol diet. In conclusion, high cholesterol diet was capable of masking the T2DM-induced osteopenia and changes in several histomorphometric parameters that indicated bone microstructural defect. Cholesterol thus explained, in part, why a decrease in BMD was not observed in T2DM, and hence delayed diagnosis of the T2DM-associated bone disease.
|
['Animals', 'Bone Density', 'Bone Diseases, Metabolic', 'Bone and Bones', 'Cholesterol, Dietary', 'Delayed Diagnosis', 'Diabetes Mellitus, Type 2', 'Dyslipidemias', 'Female', 'Growth Plate', 'Lipid Metabolism', 'Osteoblasts', 'Osteoclasts', 'Rats', 'Rats, Inbred Strains', 'X-Ray Microtomography']
| 25,200,330
|
[['B01.050'], ['G11.427.100'], ['C05.116.198', 'C18.452.104'], ['A02.835.232', 'A10.165.265'], ['D04.210.500.247.808.197.225', 'D10.212.302.347', 'D10.570.938.208.222'], ['E01.110', 'E02.760.273', 'N02.421.585.273'], ['C18.452.394.750.149', 'C19.246.300'], ['C18.452.584.500'], ['A02.835.232.251.352'], ['G03.458'], ['A11.329.629'], ['A11.329.372.700', 'A11.627.482.700'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.050.199.520.760', 'B01.050.150.900.649.313.992.635.505.700.400'], ['E01.370.350.700.810.810.900', 'E01.370.350.825.810.810.900']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Dynamics of viral hepatitis B epidemiological process in Plovdiv region.
|
Registration of viral hepatitis cases by type started in 1982 in Bulgaria, and in August 1991 Bulgaria launched a mass immunisation programme to vaccinate infants against hepatitis B. The objective of this dissertation thesis is to study the epidemiological status of hepatitis B virus (HBV) infection in Plovdiv region (the second largest administrative region in Bulgaria with a population of 683,027 people in 2011), and assess the epidemiological process dynamics as a result of the administration of a recombinant vaccine that has been used over the past two decades.
|
['Bulgaria', 'Hepatitis B', 'Hepatitis B Surface Antigens', 'Hepatitis B Vaccines', 'Humans', 'Incidence', 'Renal Dialysis', 'Vaccination']
| 24,191,410
|
[['Z01.542.248.180'], ['C01.221.250.500', 'C01.925.256.430.400', 'C01.925.440.435', 'C06.552.380.705.437'], ['D23.050.327.495.500.475'], ['D20.215.894.899.955.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.308.985.525.375', 'N01.224.935.597.500', 'N06.850.505.400.975.525.375', 'N06.850.520.308.985.525.375'], ['E02.870.300', 'E02.912.800'], ['E02.095.465.425.400.530.890', 'E05.478.550.600.890', 'N02.421.726.758.310.890', 'N06.850.780.200.425.900', 'N06.850.780.680.310.890']]
|
['Geographicals [Z]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
|
Immune enhancing effects of Lactobacillus acidophilus LAFTI L10 and Lactobacillus paracasei LAFTI L26 in mice.
|
The immune enhancing properties of Lactobacillus acidophilus LAFTI L10 and Lactobacillus paracasei LAFTI L26 in mice were investigated. Each mouse (BALB/c) was orally fed with cultures of either L. acidophilus or L. paracasei at 10(8) CFU/50 mul per day for 14 days. The effect of these strains on immunoglobulin A, interleukin-10 and interferon gamma producing cells in the gut immune system was determined by immunofluorescence assays. Systemic immune responses were analysed in mice serum upon euthanising after a 14-day feeding trial to estimate cytokines such as IL-10 and IFN-gamma by enzyme-linked immunosorbent assays. L. acidophilus and L. paracasei strains demonstrated an increase in the number of IgA producing cells, IL-10 and IFN-gamma cytokine producing cells in the small intestine. In the systemic immune response, mice fed with L. acidophilus or L. paracasei also enhanced the secretion of anti-inflammatory cytokine (IL-10) and pro-inflammatory cytokine (IFN-gamma). The results of this study suggest that L. acidophilus and L. paracasei were able to enhance specific gut and systemic immune responses in mice.
|
['Animals', 'Colony Count, Microbial', 'Enzyme-Linked Immunosorbent Assay', 'Fluorescent Antibody Technique, Direct', 'Immunoglobulin A', 'Interferon-gamma', 'Interleukin-10', 'Intestine, Small', 'Lactobacillus', 'Lactobacillus acidophilus', 'Male', 'Mice', 'Mice, Inbred BALB C', 'Random Allocation']
| 17,134,782
|
[['B01.050'], ['E01.370.225.875.220', 'E05.200.875.220'], ['E05.478.566.350.170', 'E05.478.566.380.360', 'E05.478.583.400.170', 'E05.601.470.350.170', 'E05.601.470.380.360'], ['E01.370.225.500.607.512.240.300', 'E01.370.225.750.551.512.240.300', 'E05.200.500.607.512.240.300', 'E05.200.750.551.512.240.300', 'E05.478.583.375.300'], ['D12.776.124.486.485.114.619.026', 'D12.776.124.790.651.114.619.026', 'D12.776.377.715.548.114.619.026'], ['D12.644.276.374.440.893', 'D12.644.276.374.480.615.350', 'D12.776.467.374.440.893', 'D12.776.467.374.480.615.350', 'D23.529.374.440.893', 'D23.529.374.480.615.350'], ['D12.644.276.374.465.510', 'D12.776.467.374.465.510', 'D23.529.374.465.510'], ['A03.556.124.684'], ['B03.353.750.450.475', 'B03.510.460.400.410.475.475', 'B03.510.550.450.475'], ['B03.353.750.450.475.100', 'B03.510.460.400.410.475.475.100', 'B03.510.550.450.475.100'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.338', 'B01.050.150.900.649.313.992.635.505.500.400.338'], ['E05.318.370.700', 'E05.581.500.805', 'N05.715.360.325.675', 'N06.850.520.445.700']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Health Care [N]']
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Effect of cytidine on the modification of phospholipid metabolism induced by ischemia.
|
[1-14C-]Arachidonic acid was injected into the lateral ventricle of the gerbils (meriones unguiculatus) two hours before producing brain ischemia by the bilateral ligation of the carotid arteries. Ten minutes before the carotid ligation a group of animals received an additional intraventricular injection of cold cytidine (2.5 mumol/brain). Control animals with and without cytidine, together with the ischemic group, were decapitated directly into liquid nitrogen ten minutes after carotid ligation or sham surgery. Cytidine is able to both stimulate arachidonic acid incorporation into lipids and noticeably correct the release of this acid from polar lipids induced by ischemia. Based on these findings, it is possible to assume that cytidine exerts an effect on the biosynthesis of phosphoglycerides as well as on their catabolic activities.
|
['Animals', 'Arachidonic Acid', 'Arachidonic Acids', 'Brain', 'Brain Ischemia', 'Cytidine', 'Diglycerides', 'Gerbillinae', 'Injections, Intraventricular', 'Male', 'Phospholipids', 'Plasmalogens']
| 3,108,688
|
[['B01.050'], ['D10.251.355.255.100.100', 'D10.251.355.310.166.100'], ['D10.251.355.255.100', 'D10.251.355.310.166'], ['A08.186.211'], ['C10.228.140.300.150', 'C14.907.253.092'], ['D03.383.742.680.245', 'D13.570.685.245', 'D13.570.800.286'], ['D10.351.303'], ['B01.050.150.900.649.313.992.635.300'], ['E02.319.267.530.550'], ['D10.570.755'], ['D10.570.755.375.760.400.985.820']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Insights into the alteration of osteoblast mechanical properties upon adhesion on chitosan.
|
Cell adhesion on substrates is accompanied by significant changes in shape and cytoskeleton organization, which affect subsequent cellular and tissue responses, determining the long-term success of an implant. Alterations in osteoblast stiffness upon adhesion on orthopaedic implants with different surface chemical composition and topography are, thus, of central interest in the field of bone implant research. This work aimed to study the mechanical response of osteoblasts upon adhesion on chitosan-coated glass surfaces and to investigate possible correlations with the level of adhesion, spreading, and cytoskeleton reorganization. Using the micropipette aspiration technique, the osteoblast elastic modulus was found higher on chitosan-coated than on uncoated control substrates, and it was found to increase in the course of spreading for both substrates. The cell-surface contact area was measured throughout several time points of adhesion to quantify cell spreading kinetics. Significant differences were found between chitosan and control surfaces regarding the response of cell spreading, while both groups displayed a sigmoidal kinetical behavior with an initially elevated spreading rate which stabilizes in the second hour of attachment. Actin filament structural changes were confirmed after observation with confocal microscope. Biomaterial surface modification can enhance osteoblast mechanical response and induce favorable structural organization for the implant integration.
|
['Actin Cytoskeleton', 'Aged', 'Cell Adhesion', 'Cells, Cultured', 'Chitosan', 'Coated Materials, Biocompatible', 'Elastic Modulus', 'Female', 'Humans', 'Male', 'Middle Aged', 'Osteoblasts']
| 24,987,701
|
[['A11.284.430.214.190.750.050'], ['M01.060.116.100'], ['G04.022'], ['A11.251'], ['D05.750.078.139.500', 'D09.698.211.500'], ['D25.130.420', 'J01.637.051.130.420'], ['G01.374.590.605'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['A11.329.629']]
|
['Anatomy [A]', 'Named Groups [M]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Technology, Industry, and Agriculture [J]', 'Organisms [B]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
|
Echocardiographic detection of an intact aneurysm in a horse.
|
Two-dimensional real-time echocardiographic examination of a 3-year-old Thoroughbred gelding with pleuropneumonia revealed an intact aneurysm of the right sinus of Valsalva, which was confirmed at postmortem examination. The horse had no clinical signs associated with the aneurysm.
|
['Animals', 'Aortic Aneurysm', 'Auscultation', 'Echocardiography', 'Horse Diseases', 'Horses', 'Male', 'Pleuropneumonia', 'Punctures', 'Sinus of Valsalva', 'Thorax']
| 2,211,328
|
[['B01.050'], ['C14.907.055.239', 'C14.907.109.139'], ['E01.370.600.060'], ['E01.370.350.130.750', 'E01.370.350.850.220', 'E01.370.370.380.220'], ['C22.488'], ['B01.050.150.900.649.313.984.235.472'], ['C01.748.582.473', 'C01.748.610.473', 'C08.381.677.473', 'C08.528.735.473', 'C08.730.582.473', 'C08.730.610.473'], ['E02.800', 'E04.665'], ['A07.015.114.056.847'], ['A01.923.761']]
|
['Organisms [B]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Development of rat urinary HPLC-UV profiling for metabonomic study on Liuwei Dihuang Pills.
|
Since urine contains thousands of metabolites, there are no universal analytical techniques to analyze these compounds simultaneously. Therefore, a readily obtained and simple sample pretreatment high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection urinary profiling with good reproducibility was developed for metabonomic study in this paper. The results of current study clearly showed that there were different phenotypes of metabolites based on HPLC-UV urinary profiling after administration of Liuwei Dihuang Pills (LWPs) or Carrageenan-stimulated inflammation model, and those could be conveniently discriminated by principal component analysis (PCA). In addition, the results also showed that LWPs could restore the metabolite network that disturbed by inflammation, which would be a proof of therapeutic efficacy of LWPs to inflammation by metabonomics study. Our study indicated that HPLC-UV method could be used as a potentially powerful tool for urinary profiling on metabonomic studies.
|
['Animals', 'Chromatography, High Pressure Liquid', 'Dosage Forms', 'Drugs, Chinese Herbal', 'Gene Expression Profiling', 'Male', 'Medicine, Chinese Traditional', 'Metabolome', 'Random Allocation', 'Rats', 'Rats, Sprague-Dawley', 'Reproducibility of Results', 'Spectrophotometry, Ultraviolet', 'Urinalysis']
| 19,081,221
|
[['B01.050'], ['E05.196.181.400.300'], ['D26.255', 'E05.916.250'], ['D20.215.784.500.350', 'D26.335'], ['E05.393.332'], ['E02.190.488.585.520', 'I01.076.201.450.654.558.520'], ['G03.500'], ['E05.318.370.700', 'E05.581.500.805', 'N05.715.360.325.675', 'N06.850.520.445.700'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.750'], ['E05.318.370.725', 'E05.337.851', 'N05.715.360.325.685', 'N06.850.520.445.725'], ['E05.196.712.726.802', 'E05.196.867.826.802'], ['E01.370.225.124.810', 'E01.370.390.810', 'E05.200.124.810']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Phenomena and Processes [G]', 'Health Care [N]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
|
Advantages of the enlarged middle cranial fossa approach in acoustic neurinoma surgery. A review.
|
Utilizing an enlarged middle cranial fossa approach to the cerebello-pontine angle without destruction of the labyrinth or cochlea the authors have since 1981 operated on 263 unilateral acoustic neurinomas. Tumour sizes ranged between 3 mm intrameatal and 35 mm within the cerebello-pontine angle. Complete tumour removal was accomplished in 96%. There was one postoperative mortality, and only rarely neurological complications. Excellent function of the facial nerve was obtained in 78% (in small and medium sized neurinomas 90% House I and II) and severe paralysis persisted in only 6%. Preservation of hearing was possible in 70% of the small tumours, and in 50% of the total group. Against this background comparable data of the literature are reviewed, and the indications for the enlarged midfossa approach analyzed.
|
['Adolescent', 'Adult', 'Aged', 'Aged, 80 and over', 'Deafness', 'Evoked Potentials, Auditory, Brain Stem', 'Facial Nerve', 'Hearing', 'Humans', 'Middle Aged', 'Monitoring, Intraoperative', 'Neuroma, Acoustic', 'Petrous Bone', 'Postoperative Complications', 'Retrospective Studies', 'Semicircular Canals', 'Skull', 'Vestibulocochlear Nerve']
| 1,441,980
|
[['M01.060.057'], ['M01.060.116'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['C09.218.458.341.186', 'C10.597.751.418.341.186', 'C23.888.592.763.393.341.186'], ['G07.265.216.500.370.300', 'G07.888.250.300', 'G11.561.200.500.370.300'], ['A08.800.050.050.275', 'A08.800.050.600.149', 'A08.800.800.060.275', 'A08.800.800.120.250'], ['F02.830.816.263', 'G07.888.500', 'G11.561.790.263'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['E01.370.520.510', 'E04.510'], ['C04.557.465.625.650.595.610', 'C04.557.580.600.610.595.610', 'C04.557.580.625.650.595.610', 'C04.588.614.300.015', 'C04.588.614.596.240.015', 'C09.218.807.800.675', 'C09.647.675', 'C10.292.225.750', 'C10.292.910.600'], ['A02.835.232.781.885.681'], ['C23.550.767'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['A09.246.300.663'], ['A02.835.232.781'], ['A08.800.800.120.910']]
|
['Named Groups [M]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Psychiatry and Psychology [F]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 1
| 1
| 1
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Three-dimensional quantitative assessment of lesion response to MR-guided high-intensity focused ultrasound treatment of uterine fibroids.
|
RATIONALE AND OBJECTIVES: To investigate the response after magnetic resonance-guided high-intensity focused ultrasound (MRgHIFU) treatment of uterine fibroids (UF) using a three-dimensional (3D) quantification of total and enhancing lesion volume (TLV and ELV, respectively) on contrast-enhanced MRI (ceMRI) scans.METHODS AND MATERIALS: In a total of 24 patients, ceMRI scans were obtained at baseline and 24 hours, and 6, 12, and 24 months after MRgHIFU treatment. The dominant lesion was assessed using a semiautomatic quantitative 3D segmentation technique. Agreement between software-assisted and manual measurements was then analyzed using a linear regression model. Patients were classified as responders (R) or nonresponders (NR) on the basis of their symptom report after 6 months. Statistical analysis included the paired t-test and Mann-Whitney test.RESULTS: Preprocedurally, the median TLV and ELV were 263.74 cm(3) (30.45-689.56 cm(3)) and 210.13 cm(3) (14.43-689.53 cm(3)), respectively. The 6-month follow-up demonstrated a reduction of TLV in 21 patients (87.5%) with a median TLV of 171.7 cm(3) (8.5-791.2 cm(3); P < .0001). TLV remained stable with significant differences compared to baseline (P < .001 and P = .047 after 12 and 24 months). A reduction of ELV was apparent in 16 patients (66.6%) with a median ELV of 158.91 cm(3) (8.55-779.61 cm(3)) after 6 months (P = .065). Three-dimensional quantification and manual measurements showed strong intermethod agreement for fibroid volumes (R(2) = .889 and .917) but greater discrepancy for enhancement calculations (R(2) = .659 and .419) at baseline and 6 months. No significant differences in TLV or ELV were observed between clinical R (n = 15) and NR (n = 3).CONCLUSIONS: The 3D assessment has proven feasible and accurate in the quantification of fibroid response to MRgHIFU. Contrary to ELV, changes in TLV may be representative of the clinical outcome.
|
['Adult', 'Cohort Studies', 'Contrast Media', 'Feasibility Studies', 'Female', 'Follow-Up Studies', 'High-Intensity Focused Ultrasound Ablation', 'Humans', 'Image Processing, Computer-Assisted', 'Imaging, Three-Dimensional', 'Leiomyoma', 'Magnetic Resonance Imaging, Interventional', 'Middle Aged', 'Remission Induction', 'Retrospective Studies', 'Treatment Outcome', 'Tumor Burden', 'Uterine Neoplasms']
| 26,160,057
|
[['M01.060.116'], ['E05.318.372.500.750', 'N05.715.360.330.500.750', 'N06.850.520.450.500.750'], ['D27.505.259.500', 'D27.720.259'], ['E05.318.372.550', 'E05.337.675', 'N05.715.360.330.550', 'N06.850.520.450.550'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['E02.565.280.945.399', 'E04.014.380'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['L01.224.308'], ['E01.370.350.400', 'L01.224.308.410'], ['C04.557.450.590.450'], ['E04.502.515'], ['M01.060.116.630'], ['E02.860'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['E05.041.124.892'], ['C04.588.945.418.948', 'C13.351.500.852.762', 'C13.351.937.418.875']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Information Science [L]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 1
| 0
|
Micromethod for the determination of 3-beta-HSD activity in cultured cells.
|
A modified radioassay for the determination of the 3 beta-hydroxy-delta 5-steroid dehydrogenase (3-beta-HSD) is described. The assay is based on the conversion of [3H]pregnenolone to [3H]progesterone followed by a digitonin precipitation step. The method was applied to neurons, glial cells, C6 glioma cells and adrenal tumor cells in culture. Adrenal tumor cells and C6 glioma cells showed higher enzyme activity than primary cultures of astrocytes and neurons. Dependence of enzyme activity on pH, protein concentration and reaction time was demonstrated for C6 cells. A pH optimum was shown between 7.5 and 8.1, and the reaction was linear up to 2 h. beta-oestradiol inhibited 3-beta-HSD activity completely. The assay presented is fast, highly reproducible, and offers the possibility of studying 3-beta-HSD activity in differentiating cells in culture without preparation of microsomes or extraction of reaction products.
|
['3-Hydroxysteroid Dehydrogenases', 'Animals', 'Brain', 'Cells, Cultured', 'Mice', 'Mice, Inbred BALB C', 'Neuroglia', 'Neurons', 'Tritium']
| 2,811,376
|
[['D08.811.682.047.436.350'], ['B01.050'], ['A08.186.211'], ['A11.251'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.338', 'B01.050.150.900.649.313.992.635.505.500.400.338'], ['A08.637', 'A11.650'], ['A08.675', 'A11.671'], ['D01.268.406.875', 'D01.362.340.875', 'D01.496.749.925']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Flavobacterium antarcticum sp. nov., a novel psychrotolerant bacterium isolated from the Antarctic.
|
A yellow-pigmented, Gram-negative and aerobic bacterial strain, designated AT1026(T), was isolated from a terrestrial sample from the Antarctic. Results of 16S rRNA gene sequence analysis indicated that the Antarctic isolate belonged to the genus Flavobacterium, with the highest sequence similarity to Flavobacterium tegetincola (96.4 %). Cells were non-motile, non-gliding and psychrotolerant, with optimum and maximum temperatures of about 20 and 25 degrees C. Flexirubins were absent. The major isoprenoid quinone (MK-6), predominant cellular fatty acids (iso-C(15 : 1) G, iso-C(15 : 0) and a mixture of C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH) and DNA G+C content (38 mol%) of the Antarctic isolate were consistent with those of the genus Flavobacterium. In contrast, several phenotypic characters can be used to differentiate this isolate from other flavobacteria. The polyphasic data presented in this study indicated that this isolate should be classified as a novel species in the genus Flavobacterium. The name Flavobacterium antarcticum sp. nov. is therefore proposed for the Antarctic isolate; the type strain is AT1026(T) (=IMSNU 14042(T)=KCTC 12222(T)=JCM 12383(T)).
|
['Antarctic Regions', 'Bacterial Typing Techniques', 'Cold Temperature', 'DNA, Bacterial', 'DNA, Ribosomal', 'Fatty Acids', 'Flavobacterium', 'Molecular Sequence Data', 'Phenotype', 'RNA, Ribosomal, 16S', 'Sequence Analysis, DNA', 'Soil Microbiology']
| 15,774,636
|
[['Z01.158'], ['E01.370.225.875.150.125', 'E05.200.875.150.125'], ['G01.906.595.272', 'G16.500.275.063.725.710.300', 'G16.500.750.775.710.300', 'N06.230.300.100.725.154', 'N06.230.300.100.725.710.300'], ['D13.444.308.212'], ['D13.444.308.475'], ['D10.251'], ['B03.440.080.190.250', 'B03.440.400.425.310.250'], ['L01.453.245.667'], ['G05.695'], ['D13.444.735.686.670'], ['E05.393.760.700'], ['H01.158.273.540.274.555', 'N06.850.425.300']]
|
['Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Information Science [L]', 'Disciplines and Occupations [H]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 1
|
Highly sensitive detection of hybridization of oligonucleotides to specific sequences of nucleic acids by application of fluorescence resonance energy transfer.
|
We show a new application of fluorescence resonance energy transfer (FRET) in two stages to detect specific sequences of nucleic acids. In the first stage, two fluorescently tagged oligonucleotides hybridize with a complementary target molecule to produce FRET. The sequences of the oligonucleotides and spectral properties of fluorophores are chosen to provide a basis for an efficient energy transfer. In the next step, the specificity of hybridization is tested by competition of labeled probes with an excess of unlabeled oligonucleotides of the same sequence. The resulting emission spectra, one obtained in the excess of unlabeled donor probe and the other produced in the excess of unlabeled acceptor probe, are compared with the spectrum from the first stage to look for differences in the emission pattern of the fluorescent labels. We show that it is possible to detect the existence of specific hybrids composed of the two probes and complementary target molecule even in very unfavorable conditions, such as the presence of unhybridized probes in the final reaction mixture, secondary nonacceptor quenching of donor probe fluorescence, and strong background emission of acceptor produced by its direct excitation with a donor excitation light.
|
['Binding, Competitive', 'Energy Transfer', 'Fluorescence Polarization', 'Nucleic Acid Hybridization', 'Oligonucleotide Probes', 'Spectrometry, Fluorescence', 'Titrimetry']
| 10,805,160
|
[['E05.196.080', 'G02.111.084', 'G02.111.570.120.309'], ['G01.154.240', 'G02.111.255', 'G02.216'], ['E05.196.712.516.600.390'], ['E05.393.661', 'G02.111.611'], ['D13.444.600.601', 'D27.505.259.750.600.650', 'D27.720.470.530.600.650'], ['E05.196.712.516.600.676', 'E05.196.867.726'], ['E05.196.922']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Associations between working memory, health literacy, and recall of the signs of stroke among older adults.
|
Stroke remains a major cause of mortality and disability among older adults. Although early treatment after stroke is known to reduce both mortality and disability, the first step in seeking early treatment is dependent on the rapid recognition of the signs of stroke. Recall of the signs of stroke may be dependent on factors that exist before the stroke itself. Although it is known that both working memory and health literacy decline with advancing age, these factors have not been thoroughly examined with respect to recall of the signs of stroke. Therefore, the purpose of the current study was to investigate associations between working memory, health literacy, and recall of the signs of stroke among older adults. Community dwelling older adults (?65 years of age) were recruited from two senior centers. Fifty-six participants meeting inclusion criteria provided demographic and health information and were asked to read a public service brochure listing the five warning signs of stroke. Working memory was then assessed using the Wechsler Adult Intelligence Scale 3rd Edition Working Memory Index. Health literacy was assessed by the Short Test of Functional Health Literacy in Adults. Participants' recall of the five warning signs of stroke was evaluated. The mean age was 80.4 years. The mean number of the signs of stroke recalled was 2.9 ± 1.33. Working memory and health literacy were positively correlated with recall of the signs of stroke (r = .38, p < 0.01; r = .44, p < 0.01). In a simultaneous regression, only health literacy remained a significant predictor of recall. There was no statistically significant interaction between working memory and health literacy. Findings from this study indicate that working memory and health literacy were associated with successful recall of the warning signs of stroke in older adults. Further studies are needed to determine if programs that include cognitive and literacy assessments could identify older adults who need additional support to learn and recall the signs of stroke.
|
['Aged', 'Aged, 80 and over', 'Diagnostic Self Evaluation', 'Female', 'Health Literacy', 'Humans', 'Male', 'Memory, Short-Term', 'Mental Recall', 'New York City', 'Pamphlets', 'Stroke']
| 22,955,236
|
[['M01.060.116.100'], ['M01.060.116.100.080'], ['E01.370.360', 'F01.752.747.792.220'], ['I02.233.332.186.500', 'L01.143.450.500', 'N02.421.726.407.229.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F02.463.425.540.407'], ['F02.463.425.540.641'], ['Z01.107.567.875.350.530.530', 'Z01.107.567.875.500.530.530', 'Z01.433.741'], ['L01.178.682.707'], ['C10.228.140.300.775', 'C14.907.253.855']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Psychiatry and Psychology [F]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Information Science [L]', 'Health Care [N]', 'Organisms [B]', 'Geographicals [Z]', 'Diseases [C]']
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 1
| 1
| 1
|
Quality of Life in Painful Peripheral Neuropathies: A Systematic Review.
|
Objective: Neuropathic pain is a common presenting complaint of patients with peripheral neuropathy (PN) and is considered one of the most disabling neuropathic symptoms, with detrimental effects on patients' quality of life (QoL). The aim of this review was to overview the current literature that focuses on QoL in painful PN of various aetiologies. We sought to clarify the direct effect of pain and its treatment on patients' QoL.Methodology: A systematic computer-based literature search was conducted using the PubMed database to search for papers on QoL in painful PN. Information was extracted regarding prevalence, demographics, and response to treatment where relevant.Results: We identified 66 articles eligible for inclusion. The vast majority of studies (n=47) focused on patients with diabetic PN. Other aetiologies of painful PN where QoL has been studied to date include gluten, immune-mediated, HIV, chemotherapy-induced, and chronic idiopathic axonal polyneuropathy. Pharmacological treatment is the mainstay in managing pain and has a direct positive and independent effect on the overall QoL. Other nonpharmacological approaches can also be of benefit, either alone or as adjuvant treatments, and are discussed.Conclusion: The findings demonstrate that QoL is impaired in painful PN and should not be neglected in clinical practice. Patients' pain management and subsequent impact on QoL should routinely be assessed and monitored.
|
['Humans', 'Neuralgia', 'Pain Management', 'Quality of Life']
| 31,249,636
|
[['B01.050.150.900.649.313.988.400.112.400.400'], ['C10.668.829.600', 'C23.888.592.612.664'], ['E02.745', 'N04.590.607.500'], ['I01.800', 'K01.752.400.750', 'N06.850.505.400.425.837']]
|
['Organisms [B]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Humanities [K]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 0
|
The absorption characterization effects and mechanism of Radix Angelicae dahuricae extracts on baicalin in Radix Scutellariae using in vivo and in vitro absorption models.
|
ETHNOPHARMACOLOGICAL RELEVANCE: Angelicae Dahurica(Hoffm.)Benth.&Hook.f.ex Franch.&Sav combined with Scutellaria baicalensis Georgi. has been widely used as herb-pairs in traditional Chinese medicine (TCM) to treat migraine headache and cataract, but the underlying compatibility mechanism of the two herbs remains unknown.AIM OF STUDY: In the present work, we investigated the additive or synergistic effects of absorption behavior of Radix Angelicae dahuricae extracts on baicalin, and the absorption-enhancing mechanism of Radix Angelicae dahuricae extracts on baicalin.MATERIALS AND METHODS: Total coumarins (Cou) and volatile oil (VO), as the two main components of Radix Angelicae dahuricae, were extracted by supercritical fluid extraction (SFE) further treated with liquid-liquid separation method. The absorption behavior was investigated by applying the everted gut sac technique and in situ single-pass intestinal perfusion method.RESULTS AND CONCLUSIONS: The results showed that both the Cou and the VO could improve the intestinal absorption of baicalin in vivo, and had synergistic action the enhanced absorption of baicalin. Since verapamil did not affect the P(app) and K(a) of baicalin significantly, we concluded that the absorption of Baicalin could not be an active transportation in dependent of P-glycoprotein-Mediated efflux systems. Based on intestinal absorption of drug studying was one of the efficacious methods to clarify the compatibility of principles of herb-pairs. The everted gut sac technique and in situ single-pass intestinal perfusion technique model were the effective methods to study the absorption of drug, the application of the animal model to investigating the absorption of herb-drug interactions or other relevant research purposes is envisioned.
|
['ATP Binding Cassette Transporter, Subfamily B, Member 1', 'Angelica', 'Animals', 'Coumarins', 'Drugs, Chinese Herbal', 'Female', 'Flavonoids', 'Herb-Drug Interactions', 'In Vitro Techniques', 'Intestinal Absorption', 'Intestinal Mucosa', 'Male', 'Oils, Volatile', 'Rats', 'Rats, Sprague-Dawley', 'Scutellaria baicalensis']
| 22,023,882
|
[['D12.776.157.530.100.075.063', 'D12.776.157.530.450.074.500.500.250.125', 'D12.776.395.550.020.400.153', 'D12.776.543.550.192.400.153', 'D12.776.543.585.100.200.125', 'D12.776.543.585.450.074.500.500.250.125'], ['B01.650.940.800.575.912.250.075.088'], ['B01.050'], ['D03.383.663.283.446', 'D03.633.100.150.446'], ['D20.215.784.500.350', 'D26.335'], ['D03.383.663.283.266.450', 'D03.633.100.150.266.450'], ['G07.690.773.968.755'], ['E05.481'], ['G03.015.500.374.500', 'G03.787.024.500.374.500', 'G07.203.650.372.500', 'G07.690.725.015.500.374.500', 'G10.261.353.500'], ['A03.556.124.369', 'A10.615.550.444'], ['D10.627.675'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.750'], ['B01.650.940.800.575.912.250.583.520.942.500']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Up-regulated HMGB1 in EAM directly led to collagen deposition by a PKCâ/Erk1/2-dependent pathway: cardiac fibroblast/myofibroblast might be another source of HMGB1.
|
High mobility group box 1 (HMGB1), an important inflammatory mediator, is actively secreted by immune cells and some non-immune cells or passively released by necrotic cells. HMGB1 has been implicated in many inflammatory diseases. Our previous published data demonstrated that HMGB1 was up-regulated in heart tissue or serum in experimental autoimmune myocarditis (EAM); HMGB1 blockade could ameliorate cardiac fibrosis at the last stage of EAM. And yet, until now, no data directly showed that HMGB1 was associated with cardiac fibrosis. Therefore, the aims of the present work were to assess whether (1) up-regulated HMGB1 could directly lead to cardiac fibrosis in EAM; (2) cardiac fibroblast/myofibroblasts could secrete HMGB1 as another source of high-level HMGB1 in EAM; and (3) HMGB1 blockade could effectively prevent cardiac fibrosis at the last stage of EAM. Our results clearly demonstrated that HMGB1 could directly lead to cardiac collagen deposition, which was associated with PKCâ/Erk1/2 signalling pathway; furthermore, cardiac fibroblast/myofibroblasts could actively secrete HMGB1 under external stress; and HMGB1 secreted by cardiac fibroblasts/myofibroblasts led to cardiac fibrosis via PKCâ activation by autocrine means; HMGB1 blockade could efficiently ameliorate cardiac fibrosis in EAM mice.
|
['Animals', 'Autoimmune Diseases', 'Disease Models, Animal', 'Extracellular Signal-Regulated MAP Kinases', 'Fibrillar Collagens', 'HMGB1 Protein', 'MAP Kinase Signaling System', 'Mice, Inbred BALB C', 'Myocarditis', 'Myofibroblasts', 'Osteopontin', 'Protein Kinase C beta', 'Up-Regulation']
| 24,912,759
|
[['B01.050'], ['C20.111'], ['C22.232', 'E05.598.500', 'E05.599.395.080'], ['D08.811.913.696.620.682.700.567.249', 'D12.644.360.450.169', 'D12.776.476.450.169'], ['D05.750.078.280.300', 'D12.776.860.300.250.300'], ['D12.776.260.356.300', 'D12.776.660.235.400.600.300', 'D12.776.664.235.400.600.300'], ['G02.111.820.560', 'G03.493.560', 'G04.835.560'], ['B01.050.050.199.520.520.338', 'B01.050.150.900.649.313.992.635.505.500.400.338'], ['C14.280.238.625'], ['A11.329.228.975', 'A11.620.520.500'], ['D12.644.276.374.625', 'D12.776.395.700.837', 'D12.776.467.374.625', 'D12.776.860.300.762', 'D23.529.374.625'], ['D08.811.913.696.620.682.700.725.049'], ['G02.111.905', 'G05.308.850', 'G07.690.773.998']]
|
['Organisms [B]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Antihypertensives and myocardial infarction risk: the modifying effect of history of drug use.
|
PURPOSE: Confounding by indication is common in observational studies of outcomes that treatment is intended to affect. In light of the stepped-care approach to hypertension management, we reexamined the controversy around myocardial infarction (MI) risk in relation to antihypertensive agents by considering past drug history both as a confounder and as an effect modifier.METHODS: Case-control design nested within a cohort of 19,501 adults initiating therapy with angiotensin-converting enzyme inhibitors (ACEI), calcium channel blockers (CCB) or beta-blockers in Saskatchewan (1990-93) and followed up to 1997. MI cases were identified using death certificates and hospital discharge diagnoses (ICD-9,410). Four controls were matched to each case to account for duration and timing of follow-up.RESULTS: 812 MI cases were identified, of which 26% were fatal. At first, current use of CCB and ACEI (versus beta-blockers) appeared to be associated with an increased risk of MI (RR = 2.2; 95% CI = 1.8-2.7 and RR = 1.3; CI = 1.0-1.6 respectively). Adjustment for drug use history attenuated both associations (RR = 1.6; CI = 1.1-2.2 and RR = 1.0; CI = 0.7-1.4). Moreover, the risk for CCB use disappeared when restricted to patients who had already used these agents in the past (RR = 1.1; CI = 0.77-1.7) whereas a high risk of MI for ACEI was found in digoxin users (RR = 9.4; CI = 3.2-27.5).CONCLUSION: Past drug history can be both a confounder and an effect modifier in observational studies. We found adjustment for medication history to attenuate the associations between antihypertensive agents and MI risk. In addition, the estimates significantly varied across drug history profiles thus suggesting the presence of preferential prescribing of specific drug classes to high-risk patients.
|
['Adrenergic beta-Antagonists', 'Adult', 'Aged', 'Angiotensin-Converting Enzyme Inhibitors', 'Antihypertensive Agents', 'Calcium Channel Blockers', 'Case-Control Studies', 'Cohort Studies', 'Digoxin', 'Drug Prescriptions', 'Drug Utilization', 'Female', 'Humans', 'Hypertension', 'Male', 'Middle Aged', 'Myocardial Infarction', 'Risk Assessment', 'Saskatchewan']
| 11,760,488
|
[['D27.505.519.625.050.200.200', 'D27.505.696.577.050.200.200'], ['M01.060.116'], ['M01.060.116.100'], ['D27.505.519.389.745.085'], ['D27.505.954.411.162'], ['D27.505.519.562.249', 'D27.505.696.260.500', 'D27.505.954.411.192'], ['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['E05.318.372.500.750', 'N05.715.360.330.500.750', 'N06.850.520.450.500.750'], ['D04.210.500.155.580.130.500.436', 'D04.210.500.155.580.130.688', 'D09.408.180.261.436'], ['E02.319.307', 'N02.421.668.778.500'], ['N04.452.706.477'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C14.907.489'], ['M01.060.116.630'], ['C14.280.647.500', 'C14.907.585.500', 'C23.550.513.355.750', 'C23.550.717.489.750'], ['E05.318.740.600.800.715', 'N04.452.871.715', 'N05.715.360.750.625.700.690', 'N06.850.505.715', 'N06.850.520.830.600.800.715'], ['Z01.107.567.176.858']]
|
['Chemicals and Drugs [D]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Diseases [C]', 'Geographicals [Z]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Time effects, cultural influences, and individual differences in crew behavior during the Mars-500 experiment.
|
INTRODUCTION: This note provides an overview of salient factors that could have an impact on the behavior of a crew in an isolated and confined environment during a very long-term adaptive process. We present the Mars-500 experiment, which took place in Moscow, Russia, over 520 d from June 5, 2010, to November 4, 2011. It was designed to simulate a 250-d interplanetary mission from Earth to Mars, a 30-d orbital stay with a Mars landing, and a 240-d interplanetary mission from Mars back to Earth. The six-person crew was composed of three Russians, two Europeans, and one Chinese.METHODS: We applied the ethological method based on observation, description, and quantification of nonverbal behavior expressed by actions and interactions, as well as verbal behavior expressed through positions and communications. These events were scored with The Observer XT software from video recordings made every 2 wk during a daily life activity at breakfast time and every month during a group discussion task.RESULTS: We show that the frequency of occurrences of personal actions, visual interactions, facial expressions and collateral acts are linked to certain phases, periods, and temporal points of the mission. Verbal communications in English and in Russian involve prevalent language associated with place preferences and preferential relationships among the crewmembers.DISCUSSION: We found evidence that the Mars-500 crew behavior was dependent on time, culture, and the individual.
|
['Adult', 'Communication', 'Cross-Cultural Comparison', 'Culture', 'Ecological Systems, Closed', 'Factor Analysis, Statistical', 'Humans', 'Male', 'Russia', 'Space Flight']
| 24,261,062
|
[['M01.060.116'], ['F01.145.209', 'L01.143'], ['I01.076.201.450.281', 'I01.880.853.100.257'], ['I01.076.201.450', 'I01.880.853.100'], ['G16.500.275.157.240', 'N06.230.124.240', 'N06.230.150.225'], ['E05.318.740.400', 'N05.715.360.750.350', 'N06.850.520.830.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['Z01.252.122.500', 'Z01.542.248.775'], ['J01.937.285.850']]
|
['Named Groups [M]', 'Psychiatry and Psychology [F]', 'Information Science [L]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Geographicals [Z]', 'Technology, Industry, and Agriculture [J]']
| 0
| 1
| 0
| 0
| 1
| 1
| 1
| 0
| 1
| 1
| 1
| 1
| 1
| 1
|
Statistical literacy in obstetricians and gynecologists.
|
The Obstetrician-Gynecologist Statistical Literacy Questionnaire (OGSLQ) was designed to examine physicians' understanding of various number tasks that are relevant to obstetrician-gynecologists (ob-gyns) practice. Forty-seven percent of the nationally representative, practicing ob-gyns responded. Physicians did poorly on the questions about numerical facts (e.g., number of women living with HIV/AIDS), better on questions about statistical concepts (e.g., incidence, prevalence), and best on questions about numerical relationships (e.g., convert frequency to percentage) with 0%, 7%, 36%, answering all correctly, respectively. Only 19% correctly estimated the number of U.S. women with cancer. Sixty-six percent were able to use sensitivity and specificity to choose a test option. Around 90% could translate between frequency and probability formats. Forty-nine percent of respondents were able to calculate the positive predictive value of a mammography screening test. Physicians lack some understanding of statistical literacy. It is important that we monitor physicians' statistical literacy and provide training to students and physicians.
|
['Adult', 'Female', 'Gynecology', 'Humans', 'Male', 'Obstetrics', 'Physicians', 'Professional Competence', 'Statistics as Topic']
| 22,292,459
|
[['M01.060.116'], ['H02.403.763.750', 'H02.403.810.310'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['H02.403.810.450'], ['M01.526.485.810', 'N02.360.810'], ['I02.399.630'], ['E05.318.740', 'H01.548.832', 'N05.715.360.750', 'N06.850.520.830']]
|
['Named Groups [M]', 'Disciplines and Occupations [H]', 'Organisms [B]', 'Health Care [N]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 1
| 1
| 0
|
The analgesic effect of decursinol.
|
Although decursinol, which is one of the coumarins purified from the dried roots of Angelica gigas Nakai, was previously demonstrated to have antinociceptive effects on various mouse pain models such as tail-flick, hot-plate, formalin, writhing, and several cytokine-induced pain tests, the possible involvement of its analgesic effects and non-steroidal anti-inflammatory drugs (NSAIDs) has not been clearly elucidated yet. In this study, we characterized the possible interaction between decursinol and aspirin or acetaminophen in the writhing test. The antinociceptive effects of decursinol were observed at an orally-administered dose of 50 mg/kg but not at 25 or 10 mg/kg. In addition, the analgesic effects of aspirin (ASA) and acetaminophen (APAP) were shown at an orally-administered dose of 200 mg/kg but not at 50 or 100 mg/kg. We examined the effects of decursinol on the ASA or APAP at sub-analgesic doses. Although the co-administration of decursinol and ASA did not show any differences at doses of 10 or 25 mg/kg and 50 or 100 mg/kg, respectively, synergistic effects between decursinol and APAP were observed in the group of decursinol (25 mg/kg) and APAP (100 mg/kg) co-administration. These results indicated that the analgesic effect of decursinol might be involved in supraspinal cyclooxygenase regulation that might be overlapped with APAP-induced analgesic mechanisms rather than systemic or peripheral prostaglandin modulation.
|
['Acetaminophen', 'Acetic Acid', 'Analgesics', 'Angelica', 'Animals', 'Aspirin', 'Benzopyrans', 'Butyrates', 'Disease Models, Animal', 'Drug Synergism', 'Drug Therapy, Combination', 'Male', 'Mice', 'Mice, Inbred ICR', 'Pain', 'Plant Extracts']
| 19,557,373
|
[['D02.065.199.092.040', 'D02.092.146.113.092.040'], ['D02.241.081.018.165', 'D10.251.400.045.500'], ['D27.505.696.663.850.014', 'D27.505.954.427.040'], ['B01.650.940.800.575.912.250.075.088'], ['B01.050'], ['D02.455.426.559.389.657.410.595.176'], ['D03.383.663.283', 'D03.633.100.150'], ['D02.241.081.114', 'D10.251.400.143'], ['C22.232', 'E05.598.500', 'E05.599.395.080'], ['G07.690.773.968.477'], ['E02.319.310'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.510', 'B01.050.150.900.649.313.992.635.505.500.400.510'], ['C23.888.592.612', 'F02.830.816.444', 'G11.561.790.444'], ['D20.215.784.500', 'D26.667']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Psychiatry and Psychology [F]']
| 0
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
High-field, variable-temperature M?ssbauer effect measurements on oxyhemeproteins.
|
We measured M?ssbauer spectra of human oxyhemoglobin, its isolated beta chains, and of oxymyoglobin from horse and sperm whale in fields of 4 or 6 T between 4.2 and 200 K in order to characterize the electronic state of the oxyheme complex. Diamagnetic sodium nitroprusside measured under the same conditions served as a control. The spectra of all compounds are reproduced adequately by a model that assumes a diagmagnetic iron and treats the quadrupole splitting, the asymmetry parameter and the M?ssbauer linewidth as adjustable parameters. The results provide no indication in the oxyhemeproteins of the excited triplet state that was postulated by Cerdonio and co-workers (Cerdonio, M., Congiu-Castellano, A., Mogno, F., Pispisa, B., Romani, G.L. and Vitale, S. (1977) Proc. Natl. Acad. Sci. USA 74, 398-400) on the basis of susceptibility measurements on oxyhemoglobin.
|
['Humans', 'Myoglobin', 'Oxyhemoglobins', 'Spectrometry, Gamma', 'Temperature']
| 6,509,067
|
[['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.776.210.500.588', 'D12.776.422.316.940'], ['D12.776.124.400.707', 'D12.776.422.316.762.687'], ['E05.196.867.776', 'E05.799.801'], ['G01.906.595', 'G16.500.275.063.725.710', 'G16.500.750.775.710', 'N06.230.150.450', 'N06.230.300.100.725.710']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Identification of the protein encoded by the transposable element Tn3 which is required for its transposition.
|
Protein products have now been identified which account for the entire coding capacity of the transposable element Tn3. Mutations in Tn3 have allowed us to map the genes encoding each of these peptides and to identify their role in transposition. We have found that only a single Tn3-encoded peptide is required for transposition. Expression of this peptide is repressed by the product of a second gene, which is itself autogenously regulated.
|
['Bacterial Proteins', 'Bacteriocin Plasmids', 'DNA Transposable Elements', 'DNA, Bacterial', 'Escherichia coli', 'Genes', 'Genes, Regulator', 'Molecular Weight', 'Plasmids', 'Transcription, Genetic']
| 390,401
|
[]
|
[]
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Decellularized human cadaveric dermis provides a safe alternative for primary inguinal hernia repair in contaminated surgical fields.
|
BACKGROUND: Repair options for inguinal hernias in contaminated surgical fields include local tissue repair or mesh repair. The former is associated with higher hernia recurrence rates. The latter generally is avoided because of an unacceptably high infection rate. AlloDerm (Lifecell Corp., Branchburg, NJ) can potentially provide a strong repair resistant to infection. The aim of our study was to evaluate if AlloDerm provides a safe alternative for inguinal hernia repair in contaminated surgical fields.METHODS: We retrospectively reviewed the medical records of all patients who underwent inguinal hernia repair with AlloDerm from December 2004 to April 2006. Demographics, risk factors, perioperative data, and recurrence rates were evaluated.RESULTS: Twelve patients with contaminated surgical fields underwent inguinal hernia repair with AlloDerm. Serious perioperative complications occurred in only 1 patient. Two patients developed superficial wound infections (treated conservatively). There were no mortalities. No hernia recurrence has been documented with a follow-up period of 3 to 17 months (median, 9 mo).CONCLUSIONS: AlloDerm provides a safe alternative for inguinal hernia repair in contaminated surgical fields.
|
['Aged', 'Aged, 80 and over', 'Collagen', 'Digestive System Surgical Procedures', 'Emergency Medical Services', 'Hernia, Femoral', 'Hernia, Inguinal', 'Humans', 'Length of Stay', 'Male', 'Middle Aged', 'Postoperative Complications', 'Recurrence', 'Retrospective Studies', 'Risk Factors', 'Serum Albumin', 'Skin, Artificial', 'Tomography, X-Ray Computed']
| 17,071,174
|
[['M01.060.116.100'], ['M01.060.116.100.080'], ['D05.750.078.280', 'D12.776.860.300.250'], ['E04.210'], ['N02.421.297'], ['C23.300.707.374.750'], ['C23.300.707.374.875'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.760.400.480', 'N02.421.585.400.480'], ['M01.060.116.630'], ['C23.550.767'], ['C23.550.291.937'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['D12.776.034.841', 'D12.776.124.727'], ['E07.858.082.800'], ['E01.370.350.350.810', 'E01.370.350.600.350.700.810', 'E01.370.350.700.700.810', 'E01.370.350.700.810.810', 'E01.370.350.825.810.810']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Diseases [C]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Inhibition of microRNA-21-3p suppresses proliferation as well as invasion and induces apoptosis by targeting RNA-binding protein with multiple splicing through Smad4/extra cellular signal-regulated protein kinase signalling pathway in human colorectal cancer HCT116 cells.
|
MicroRNA-21-3p (miR-21-3p), the passenger strand of pre-mir-21, has been found to be high-expressing in various cancers and to be associated with tumour malignancy, which is proposed as a novel focus in malignant tumours. Colorectal cancer (CRC), currently known as one of the most prevalent malignancy, is a leading cause of cancer death. This study aimed to investigate the key role of miR-21-3p in CRC by inhibiting its expression using transfection with miR-21-3p inhibitors into human CRC HCT116 cells. Results showed that the expression of miR-21-3p was higher than other CRC cells used in the study including Lovo, HT29, Colo320 and SW480 cells, inhibition of which suppressed the proliferation and induced cell cycle arrest in HCT116 cells. Besides, transfection with miR-21-3p inhibitors also attenuated cell migration and invasion, and induced apoptosis as well. Moreover, luciferase assay confirmed RBPMS as a direct target of miR-21-3p in HCT116 cells. Further, miR-21-3p inhibitors increased the nuclear accumulation of Smad4 and reduced phosphorylation of ERK. Interestingly, we found that silence of RBPMS using RNA interference (siRNA) not only elevated the cell viability but also increased the phosphorylation of ERK and reversed the nuclear accumulation of Smad4 induced by miR-21-3p inhibitors in HCT116 cells. Data suggest that inhibition of miR-21-3p suppresses cell proliferation, invasion as well as migration and induces apoptosis by directly targeting RBPMS through Smad4/ERK signalling pathway in HCT116 cells. Our study demonstrates miR-21-3p as a potent target for suppressing tumour progression of CRC which may have implications in CRC therapy in the future.
|
['Apoptosis', 'Cell Movement', 'Cell Proliferation', 'Colorectal Neoplasms', 'Extracellular Signal-Regulated MAP Kinases', 'HCT116 Cells', 'Humans', 'MAP Kinase Signaling System', 'MicroRNAs', 'Neoplasm Invasiveness', 'RNA-Binding Proteins', 'Smad4 Protein']
| 29,542,167
|
[['G04.146.954.035'], ['G04.198', 'G07.568.500.180'], ['G04.161.750', 'G07.345.249.410.750'], ['C04.588.274.476.411.307', 'C06.301.371.411.307', 'C06.405.249.411.307', 'C06.405.469.158.356', 'C06.405.469.491.307', 'C06.405.469.860.180'], ['D08.811.913.696.620.682.700.567.249', 'D12.644.360.450.169', 'D12.776.476.450.169'], ['A11.251.210.190.380', 'A11.251.860.180.380'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G02.111.820.560', 'G03.493.560', 'G04.835.560'], ['D13.150.650.319', 'D13.444.735.150.319', 'D13.444.735.790.552.500'], ['C04.697.645', 'C23.550.727.645'], ['D12.776.157.725', 'D12.776.664.962'], ['D12.644.360.024.334.750', 'D12.776.157.057.170.750', 'D12.776.260.713.750', 'D12.776.476.024.428.750', 'D12.776.624.776.760', 'D12.776.930.806.750']]
|
['Phenomena and Processes [G]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Organisms [B]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[Urinary retention caused by female urethral diverticulum].
|
We report a 49-year-old female who had circumferential urethral diverticulum. She was admitted to our department for urinary retention. A cystic lesion surrounding the urethra had been detected by magnetic resonance imaging. We diagnosed the lesion as a urethral diverticulum after injecting dye to the cystic mass from vagina and confirming its discharge from the urethra at the pubic side by urethroscopy. Transvaginal resection of the diverticulum was peformed. In general, 4% of female urethral diverticula cause urinary retention. On the other hand, 1% of female bladder outlet obstruction is caused by urethral diverticula. It is neccessary for us to keep in mind urethral diverticulum when we evaluate female patients with urinary retention.
|
['Coloring Agents', 'Diagnostic Imaging', 'Diverticulum', 'Endoscopy', 'Female', 'Humans', 'Indigo Carmine', 'Middle Aged', 'Treatment Outcome', 'Urethral Diseases', 'Urinary Retention']
| 18,051,810
|
[['D27.720.233'], ['E01.370.350'], ['C06.405.205.282.750', 'C23.300.415'], ['E01.370.388.250', 'E04.502.250'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D03.633.100.473.396'], ['M01.060.116.630'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['C12.777.767', 'C13.351.968.767'], ['C12.777.934.880', 'C13.351.968.934.880']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Organisms [B]', 'Named Groups [M]', 'Health Care [N]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.