Title
stringlengths 1
395
⌀ | abstractText
stringlengths 57
5.98k
| meshMajor
stringlengths 14
1.03k
| pmid
int64 22
33.2M
| meshid
stringlengths 2
3.14k
| meshroot
stringlengths 2
421
| A
int64 0
1
| B
int64 0
1
| C
int64 0
1
| D
int64 0
1
| E
int64 0
1
| F
int64 0
1
| G
int64 0
1
| H
int64 0
1
| I
int64 0
1
| J
int64 0
1
| L
int64 0
1
| M
int64 0
1
| N
int64 0
1
| Z
int64 0
1
|
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Successful treatment with infliximab in a patient with Diffuse Subretinal Fibrosis syndrome.
|
PURPOSE: To report a case of Diffuse Subretinal Fibrosis (DSF) syndrome refractory to immunosuppressive therapy who was successfully treated with anti-tumor necrosis factor-alpha (TNF)-alpha monoclonal antibodies infliximab.DESIGN: Interventional case report.METHODS: A 23-year-old male with bilateral choroiditis presented sudden dimness of vision associated to nasal scotoma in his right eye. Complete ophthalmic examination with appropriated clinical and laboratorial evaluations was carried out during all follow-up. Inform consent statement and Internal Ethics Board approval were also obtained for an open-label therapy schedule.RESULTS: Extensive temporal subretinal lesion in his right eye and DSF in the left eye were observed. The patient received intravenous infliximab infusion (5 mg/kg) schema. Four weeks after starting treatment his visual acuity improved with decrease in ocular inflammation.CONCLUSIONS: This report describes a case of DSF syndrome that responded remarkably well to infliximab treatment suggesting that TNF-alpha could play an important pathogenetic role in this syndrome.
|
['Adult', 'Anti-Inflammatory Agents', 'Antibodies, Monoclonal', 'Choroiditis', 'Fibrosis', 'Humans', 'Infliximab', 'Infusions, Intravenous', 'Male', 'Retina', 'Retinal Diseases', 'Spondylarthropathies', 'Syndrome', 'Tumor Necrosis Factor-alpha']
| 17,317,410
|
[['M01.060.116'], ['D27.505.954.158'], ['D12.776.124.486.485.114.224', 'D12.776.124.790.651.114.224', 'D12.776.377.715.548.114.224'], ['C11.941.160.478', 'C11.941.879.780.900.300'], ['C23.550.355'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.776.124.486.485.114.224.608', 'D12.776.124.790.651.114.224.537', 'D12.776.377.715.548.114.224.642'], ['E02.319.267.082.500', 'E02.319.267.510.590'], ['A09.371.729'], ['C11.768'], ['C05.116.900.853.625.800', 'C05.550.114.865.800'], ['C23.550.288.500'], ['D12.644.276.374.500.800', 'D12.644.276.374.750.626', 'D12.776.124.900', 'D12.776.395.930', 'D12.776.467.374.500.800', 'D12.776.467.374.750.626', 'D23.529.374.500.800', 'D23.529.374.750.626']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
[Growing teratoma syndrome].
|
Appearance of residual masses following chemotherapy of retroperitoneal metastasis from testicle's non-seminomatous germinal cell tumour does not always imply we are facing an active tumoral disease. When exeresis of these masses discovers the presence of a mature teratoma during the histological study, what we have is the so called Growing Teratoma Syndrome. Such teratoma is unresponsive to chemotherapy but the patient can be cured by means of a full exeresis of the residual mass, thus the relevance of having into account the likelihood of this pathology. The paper presents the case of a patient with retroperitoneal non-seminomatous testicle tumour and metastasis which, following chemotherapy, presented a residual mass with normal serological markers. After the exeresis it was found out that it was made up by mature teratoma. Four months after rescue surgery, the patient remains asymptomatic, has normal markers and no signs of neoplastic recurrence.
|
['Adult', 'Antineoplastic Combined Chemotherapy Protocols', 'Combined Modality Therapy', 'Humans', 'Lymph Node Excision', 'Male', 'Orchiectomy', 'Retroperitoneal Neoplasms', 'Teratoma', 'Testicular Neoplasms']
| 1,807,117
|
[['M01.060.116'], ['E02.183.750.500', 'E02.319.077.500', 'E02.319.310.037'], ['E02.186'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E04.446'], ['E04.270.282.679', 'E04.950.165.679', 'E04.950.774.860.618'], ['C04.588.033.731'], ['C04.557.465.910'], ['C04.588.322.762', 'C04.588.945.440.915', 'C12.294.260.937', 'C12.758.409.937', 'C19.344.762', 'C19.391.829.782']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Evolution of body mass index in two historical series of adolescents.
|
OBJECTIVE: To verify the evolution of body mass index (BMI) between two studies of adolescent populations.METHODS: Data on the BMI of 8,020 adolescents aged 10 to 15 years living in the city of S?o Paulo, Brazil, and enrolled on the 2005 study entitled "The nutritional profile of adolescents at public and private schools in S?o Paulo" were compared with data from the 1989 National Nutrition and Health Census (PNSN - Pesquisa Nacional sobre Sa?de e Nutri??o). Binomial testing was used to compare proportions once both data sets had been transformed into percentiles.RESULTS: Comparing the two surveys, significant increases were identified in 85th and 95th percentile BMI values for male adolescents aged 10 to 15 years and for female adolescents aged 10 to 14 years. Analysis of the difference between the 5th and 95th BMI percentiles of the S?o Paulo and PNSN samples indicates that there was probably an increase in the number of adolescents in the higher BMI ranges in S?o Paulo in relation to the PNSN survey.CONCLUSIONS: These results demonstrate a tendency for the adolescents observed mean BMI values to increase during the period between the two surveys, indicating a need for increased monitoring of this measurement as a form of preventing overweight in this population.
|
['Adolescent', 'Age Distribution', 'Age Factors', 'Biological Evolution', 'Body Mass Index', 'Body Weight', 'Brazil', 'Child', 'Female', 'Humans', 'Male', 'Nutrition Surveys', 'Nutritional Status', 'Obesity', 'Prevalence', 'Sex Distribution', 'Sex Factors']
| 17,426,871
|
[['M01.060.057'], ['I01.240.050', 'N01.224.033', 'N06.850.505.400.050'], ['N05.715.350.075', 'N06.850.490.250'], ['G05.045', 'G16.075'], ['E01.370.600.115.100.125', 'E05.041.124.125', 'G07.100.100.125', 'N06.850.505.200.100.175'], ['C23.888.144', 'E01.370.600.115.100.160.120', 'E05.041.124.160.750', 'G07.100.100.160.120', 'G07.345.249.314.120'], ['Z01.107.757.176'], ['M01.060.406'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.308.980.485', 'N05.715.360.300.800.469', 'N06.850.505.616', 'N06.850.520.308.980.469'], ['G07.203.650.650', 'N01.224.425.525'], ['C18.654.726.500', 'C23.888.144.699.500', 'E01.370.600.115.100.160.120.699.500', 'G07.100.100.160.120.699.500'], ['E05.318.308.985.525.750', 'N01.224.935.597.750', 'N06.850.505.400.975.525.750', 'N06.850.520.308.985.525.750'], ['I01.240.800', 'N01.224.803', 'N06.850.505.400.850'], ['N05.715.350.675', 'N06.850.490.875']]
|
['Named Groups [M]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Geographicals [Z]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 1
|
Cooperative cleavage of the R peptide in the Env trimer of Moloney murine leukemia virus facilitates its maturation for fusion competence.
|
The spike protein of murine leukemia virus, MLV, is made as a trimer of the Env precursor. This is primed for receptor-induced activation of its membrane fusion function first by cellular furin cleavage in the ectodomain and then by viral protease cleavage in the endodomain. The first cleavage separates the peripheral surface (SU) subunit from the transmembrane (TM) subunit, and the latter releases a 16-residue-long peptide (R) from the TM endodomain. Here, we have studied the distribution of R peptide cleavages in the spike TM subunits of Moloney MLV preparations with partially R-peptide-processed spikes. The spikes were solubilized as trimers and separated with an R peptide antibody. This showed that the spikes were either uncleaved or cleaved in all of its TM subunits. Further studies showed that R peptide cleavage-inhibited Env mutants, L(649)V and L(649)I, were rescued by wild-type (wt) Env in heterotrimeric spikes. These findings suggested that the R peptide cleavages in the spike are facilitated through positive allosteric cooperativity; i.e., the cleavage of the TM subunit in one Env promoted the cleavages of the TMs in the other Envs. The mechanism ensures that protease cleavage in newly released virus will generate R-peptide-cleaved homotrimers rather than heterotrimeric intermediates. However, using a cleavage site Env mutant, L(649)R, which was not rescued by wt Env, it was possible to produce virus with heterotrimers. These were shown to be less fusion active than the R-peptide-cleaved homotrimers. Therefore, the cooperative cleavage will speed up the maturation of released virus for fusion competence.
|
['Gene Products, env', 'Moloney murine leukemia virus', 'Protein Processing, Post-Translational', 'Virus Internalization', 'Virus Release']
| 21,228,228
|
[['D12.776.775.320', 'D12.776.964.775.325', 'D12.776.964.970.880.325'], ['B04.613.807.375.525.596', 'B04.820.650.375.525.596'], ['G02.111.660.871.790.600', 'G02.111.691.600', 'G03.734.871.790.600', 'G05.308.670.600'], ['G06.920.881'], ['G06.920.913']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[Evaluation of 99mTc-ECD SPECT/CT brain imaging with scenium analysis in patients with generalized anxiety disorder].
|
OBJECTIVE: To assess the perfusion changes in brains of patients with varying levels of generalized anxiety disorder (GAD).METHODS: A total of 38 GAD outpatients of Department of Psychiatry at our hospital from February to August, 2014 and 10 healthy controls received a 99mTC-ECD SPECT/CT scan with scenium analysis. Differences between brain perfusion and anxiety levels were analyzed by SPSS 16. 0 with one-way ANOVA, Pearson's Chi-square, t test and Spearman's correlation.RESULTS: They were grouped according to the levels of anxiety severity, i. e. mild (n = 11) , moderate (n = 16) and severe (n = 11). They had significantly lower blood flow in right superior frontal medial gyrus, right precuneus, right putamen, bilateral paracentral lobule and bilateral supplementary motor area (t = -2.19, -2.14, -2.22, -2.34, -2.08, -3.26, -2.72, P < 0.05). Individuals had significantly greater blood flow in mild group than those of control group in right olfactory (t = 2.09 P = 0.05). Individuals of moderate group had significantly lower blood flow than those of control group in left superior frontal gyrus medial orbital, right superior frontal gyrus medial orbital and left supplementary motor area, but greater in right olfactory (t = -2.16, -2.24, -2.49, 2.17, P = 0.04, 0.04, 0.02, 0.04). Individuals had lower blood flow in severe group than those of control group in left frontal lobe, right putamen, left paracentral lobule, right paracentral lobule, left precuneus, right precuneus, left parietal lobe, left precentral, right precentral, right postcentral, left rolandic operculum, left supplementary motor area, right supplementary motor area and left central region (t = -2.32, -2.11, -3.16, -2.61, -2.39, -2.18, -2.32, -2.67, -2.14, -2.11, -2.25, -4.38, -3.54, -2.38, P = 0.03, 0.05, 0.01, 0.02, 0.03, 0.04, 0.03, 0.02, 0.05, 0.05, 0.04, 0.00, 0.00, 0.03). Statistical differences existed in right middle frontal gyms orbital part (mild: 0.96 ± 0.07, moderate: 1.03 ± 0.06, severe: 0.98 ± 0.08, P = 0.04) and left paracentral (mild: 0.91 ± 0.07, moderate: 093 ± 004 severe: 0.87 ± 0.07, P = 0.02). There was a tendency of negative correlation between perfusion in right middle cingulate and paracingulate gyri, left precuneus, right precuneus and left thalamus and anxiety scores by Spearman's correlation analysis (r = -0.28, -0.28, -0.27, -0.29, P = 0.09, 0.09, 0.10, 0.07).CONCLUSION: Scenium software provides quantitative measurements for diagnosis of GAD in different anxiety levels. Also larger samples are required for confirming the results in further studies.
|
['Anxiety Disorders', 'Brain', 'Cysteine', 'Humans', 'Organotechnetium Compounds', 'Tomography, Emission-Computed, Single-Photon', 'Tomography, X-Ray Computed']
| 26,506,714
|
[['F03.080'], ['A08.186.211'], ['D02.886.030.230', 'D02.886.489.155', 'D12.125.154.299', 'D12.125.166.230'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D02.691.825'], ['E01.370.350.350.800.800', 'E01.370.350.600.350.800.800', 'E01.370.350.710.800.800', 'E01.370.350.825.800.800', 'E01.370.384.730.800.800'], ['E01.370.350.350.810', 'E01.370.350.600.350.700.810', 'E01.370.350.700.700.810', 'E01.370.350.700.810.810', 'E01.370.350.825.810.810']]
|
['Psychiatry and Psychology [F]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
The First Additional Port During Single-Incision Laparoscopic Cholecystectomy.
|
Background and Objectives: Single-incision laparoscopic cholecystectomy (SILC) has become increasingly popular. Regarding the difficulties of SILC in acute cholecystitis, additional port insertion is sometimes required. However, appropriate locations for additional port insertion have not been well studied. In the present study, the safety and effectiveness of the first additional port insertion in the epigastric region during SILC was assessed.Methods: Additional port insertions were needed in 52 of 113 patients who underwent SILC for acute cholecystitis. The first port was inserted in the epigastric region and the second (if required) was inserted in the right lateral subcostal area. A drainage catheter was positioned through the epigastric port.Results: One additional port was inserted in 43 patients and two additional ports were inserted in 9 patients. Mean operation time was 45.0 minutes in the Pure SILC group and 83.3 minutes in Additional Port group. Mean hospital stay was 3.7 days in the Pure SILC group and 5.9 days in Additional Port group. There was no open conversion. Intra-operative (n = 5) and postoperative bile leakages (n = 2) were identified in six patients. Timing of operation after onset of symptoms was significantly greater in the group with bile duct injury than in those without bile duct injury in patients who required additional ports.Conclusions: The first additional port in the epigastric area during SILC for acute cholecystitis helps to complete the operation without open conversion. However, the procedure can be performed safely in selective patients with relatively short duration of symptoms.
|
['Adult', 'Bile Ducts', 'Cholecystectomy, Laparoscopic', 'Cholecystitis, Acute', 'Female', 'Humans', 'Length of Stay', 'Male', 'Middle Aged', 'Operative Time', 'Patient Selection', 'Postoperative Complications', 'Treatment Outcome']
| 32,518,480
|
[['M01.060.116'], ['A03.159.183'], ['E04.210.120.172.140', 'E04.502.250.520.160'], ['C06.130.564.263.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.760.400.480', 'N02.421.585.400.480'], ['M01.060.116.630'], ['E04.614.374.500', 'N02.421.585.753.374.500'], ['E05.581.500.653', 'N04.590.731'], ['C23.550.767'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Named Groups [M]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Organisms [B]', 'Health Care [N]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
[The diverse effects of the combined action of cadmium chloride and ionizing radiation on the content of metallothioneins in mouse bone marrow and liver].
|
It was shown that introduction of cadmium chloride (0.75 mg/kg Cd2+) in combination with gamma irradiation (8.5 Gy) of mice increases the level of metallothioneines (MTs) in the bone marrow and liver of mice. The maximum effect was observed in 24-30 h after the performance. Similarly, irradiation with the doses of 3 to 10 Gy resulted in an increase in the contents of both MT isoforms (MT1 and MT2) in the bone marrow in 24 h. A combined action of gamma irradiation and a heavy metal caused an additive effect on the MT content in the bone marrow, whereas the MT content in liver was 2 times lower than that predicted theoretically. A possible mechanism of the discovered phenomenon was discussed. Supposedly, it is associated with different degrees of the radiation-induced inhibition of the MT1 and MT2 expression by cadmium ions.
|
['Animals', 'Bone Marrow', 'Bone Marrow Cells', 'Cadmium Chloride', 'Carcinogens', 'Dose-Response Relationship, Radiation', 'Gamma Rays', 'Liver', 'Male', 'Metallothionein', 'Mice', 'Mice, Inbred C57BL', 'Mice, Inbred CBA', 'Radiation Tolerance', 'Time Factors']
| 9,181,961
|
[['B01.050'], ['A15.382.216'], ['A11.148', 'A15.378.316'], ['D01.142.175', 'D01.210.450.150.125'], ['D27.888.569.100'], ['E05.799.513.500', 'G01.750.740.500', 'G04.712.500', 'G07.225', 'G07.738.500', 'N06.850.810.250.180'], ['G01.358.500.505.300', 'G01.750.250.300', 'G01.750.750.400'], ['A03.620'], ['D12.776.556.670'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.420', 'B01.050.150.900.649.313.992.635.505.500.400.420'], ['B01.050.050.199.520.520.440', 'B01.050.150.900.649.313.992.635.505.500.400.440'], ['G04.712', 'G07.738'], ['G01.910.857']]
|
['Organisms [B]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
[Some neuroendocrinological changes in rats of cataleptic strain GC. Influences of ontogenesis and generation of breeding].
|
The content of biogenic amines: dopamine, noradrenaline and serotonine, in rats of cataleptic strain GC as compared with the control strain Wistar at the age of 1 and 5 months is decreased, the maximal decrease being found in the so-called "nervous" animals. The aldosterone content was decreased at 5 month age in the GC rats. The testosterone content at the age of 1 month in GC rats does not differ from that in Wistar rats, but at the age of 5 months it was decreased as compared to Wistar, the maximal decrease being found again in "nervous" GC rats. The data obtained point to peculiarities of ontogenetic regulation and to commonness of mechanisms of catalepsy and "nervousness" in GC rats.
|
['Aging', 'Aldosterone', 'Animals', 'Biogenic Amines', 'Catalepsy', 'Disease Models, Animal', 'Neurosecretory Systems', 'Rats', 'Rats, Inbred Strains', 'Rats, Wistar', 'Species Specificity', 'Testosterone']
| 16,813,156
|
[['G07.345.124'], ['D04.210.500.745.745.654.062', 'D06.472.040.585.353.118'], ['B01.050'], ['D02.092.211'], ['C10.597.350.200', 'C23.888.592.350.200'], ['C22.232', 'E05.598.500', 'E05.599.395.080'], ['A06.688', 'A08.713'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.050.199.520.760', 'B01.050.150.900.649.313.992.635.505.700.400'], ['B01.050.150.900.649.313.992.635.505.700.900'], ['G16.824'], ['D04.210.500.054.079.429.824', 'D06.472.334.851.968.984']]
|
['Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
An analysis of computed tomographic brain scans. A computer-based study.
|
A microcomputer was used to analyse prospectively 1,000 sequential computed tomographic (CT) brain scans to assess whether the scanner was being used in a cost-effective and efficient manner. Of the patients scanned 58% had cerebral lesions. All the usual indications for CT examination were shown to be worth while, the exception being psychiatric cases.
|
['Adolescent', 'Adult', 'Aged', 'Brain Diseases', 'Brain Injuries', 'Brain Neoplasms', 'Child', 'Child, Preschool', 'Cost-Benefit Analysis', 'Female', 'Humans', 'Infant', 'Infant, Newborn', 'Male', 'Microcomputers', 'Middle Aged', 'Tomography, X-Ray Computed']
| 3,090,717
|
[['M01.060.057'], ['M01.060.116'], ['M01.060.116.100'], ['C10.228.140'], ['C10.228.140.199', 'C10.900.300.087', 'C26.915.300.200'], ['C04.588.614.250.195', 'C10.228.140.211', 'C10.551.240.250'], ['M01.060.406'], ['M01.060.406.448'], ['N03.219.151.125'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703'], ['M01.060.703.520'], ['L01.224.230.260.550'], ['M01.060.116.630'], ['E01.370.350.350.810', 'E01.370.350.600.350.700.810', 'E01.370.350.700.700.810', 'E01.370.350.700.810.810', 'E01.370.350.825.810.810']]
|
['Named Groups [M]', 'Diseases [C]', 'Health Care [N]', 'Organisms [B]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 1
| 0
|
Galectin-1 promotes lung cancer tumor metastasis by potentiating integrin á6â4 and Notch1/Jagged2 signaling pathway.
|
Lung cancer is a major cancer, leading in both incidence and mortality in the world, and metastasis underlies the majority of lung cancer-related deaths. Galectin-1, a glycan-binding protein, has been shown to be overexpressed in lung cancer and involved in tumor-mediated immune suppression. However, the functional role of galectin-1 in lung cancer per se remains unknown. We demonstrate that ectopic expression of galectin-1 in a low-metastatic CL1-0 lung cancer cell line promotes its migration, invasion and epithelial-mesenchymal transition. Conversely, we also show that suppression of galectin-1 expression in highly invasive CL1-5 and A549 cells inhibits migration and invasion of lung cancer cell and causes a mesenchymal-epithelial transition. These effects may be transduced by increasing the expression of integrin á6â4 and Notch1/Jagged2, which in turn co-operates in the phosphorylation of AKT. The effects of galectin-1 on cancer progression are reduced when integrin â4 and Notch1 are absent. Further study has indicated that galectin-1 knockdown prevents the spread of highly metastatic Lewis lung carcinoma in vivo. Our study suggests that galectin-1 represents a crucial regulator of lung cancer metastasis. Thus, the detection and targeted treatment of galectin-1-expressing cancer serves as a new therapeutic target for lung cancer.
|
['Animals', 'Carcinoma, Lewis Lung', 'Cell Line, Tumor', 'Cell Movement', 'Epithelial-Mesenchymal Transition', 'Galectin 1', 'Humans', 'Integrin alpha6beta4', 'Integrin beta4', 'Intercellular Signaling Peptides and Proteins', 'Jagged-2 Protein', 'Lung Neoplasms', 'Membrane Proteins', 'Mice', 'Mice, Inbred C57BL', 'Neoplasm Invasiveness', 'Neoplasm Metastasis', 'Neoplasm Transplantation', 'Phosphorylation', 'Proto-Oncogene Proteins c-akt', 'RNA Interference', 'RNA, Small Interfering', 'Receptor, Notch1', 'Signal Transduction']
| 23,389,289
|
[['B01.050'], ['C04.557.470.200.280', 'C04.619.230'], ['A11.251.210.190', 'A11.251.860.180'], ['G04.198', 'G07.568.500.180'], ['G04.356.500'], ['D12.776.503.307.100'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.776.543.750.705.408.460.170', 'D12.776.543.750.705.876.750'], ['D12.776.543.750.705.408.200.800'], ['D12.644.276', 'D12.776.467', 'D23.529'], ['D12.644.276.930.750', 'D12.776.157.125.797.750', 'D12.776.543.800.750', 'D23.529.930.750'], ['C04.588.894.797.520', 'C08.381.540', 'C08.785.520'], ['D12.776.543'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.420', 'B01.050.150.900.649.313.992.635.505.500.400.420'], ['C04.697.645', 'C23.550.727.645'], ['C04.697.650', 'C23.550.727.650'], ['E05.624'], ['G02.111.665', 'G02.607.780', 'G03.796'], ['D08.811.913.696.620.682.700.755', 'D12.776.476.565', 'D12.776.624.664.700.168'], ['G05.308.203.374.790'], ['D13.150.650.700', 'D13.444.735.150.700', 'D13.444.735.790.552.875'], ['D12.776.543.750.725.500', 'D12.776.930.770.500'], ['G02.111.820', 'G04.835']]
|
['Organisms [B]', 'Diseases [C]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Oxidative stress in the blood of farm workers following intensive pesticide exposure.
|
The aim of this study was to evaluate oxidative stress in workers who formulate organophosphate, synthetic pyrethroid and carbamate pesticides. In this survey, blood erythrocytes from a group of 94 pesticide-formulating workers (at least 5-years experience in pest-control in apple and cherry production) and 45 control subjects were examined for oxidative stress parameters. The control group was composed of 45 healthy people living in the same region with no exposure to pesticides. Lipid peroxidation level, catalase, superoxide dismutase and glutathione peroxidase activities in erythrocytes were analysed as biomarkers of oxidative stress. In addition, the acetylcholinesterase activity was measured as a biomarker of toxicity. Results indicated that chronic exposure to organophosphate, synthetic pyrethroid and carbamate pesticides were associated with increased activities of catalase, SOD and lipid peroxidation in erythrocytes (p < 0.05). Acetylcholinesterase activity did not show any significant differences between the two groups (p > 0.05). It is concluded that human chronic exposure to pesticides may result in stimulated antioxidant enzymes.
|
['Adult', 'Agriculture', 'Biomarkers', 'Carbamates', 'Cholinesterase Inhibitors', 'Erythrocytes', 'Female', 'Fruit', 'Humans', 'Insecticides', 'Lipid Peroxidation', 'Male', 'Occupational Exposure', 'Organophosphates', 'Oxidative Stress', 'Oxidoreductases', 'Pesticides', 'Pyrethrins', 'Time Factors', 'Turkey', 'Workforce']
| 21,450,927
|
[['M01.060.116'], ['J01.040'], ['D23.101'], ['D02.241.081.251'], ['D27.505.519.389.275', 'D27.505.519.625.120.300', 'D27.505.696.577.120.300'], ['A11.118.290', 'A11.443.240', 'A15.145.229.334'], ['A18.024.500', 'G07.203.300.562', 'J02.500.562'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D27.720.031.700.491', 'D27.888.723.491'], ['G02.111.515', 'G03.295.531.587'], ['N06.850.460.350.600'], ['D02.705.400'], ['G03.673', 'G07.775.750'], ['D08.811.682'], ['D27.720.031.700', 'D27.888.723'], ['D02.455.849.575.188.750'], ['G01.910.857'], ['Z01.252.245.500.850'], ['N04.452.525']]
|
['Named Groups [M]', 'Technology, Industry, and Agriculture [J]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Health Care [N]', 'Geographicals [Z]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 1
| 1
|
Effect of pre- and post-combined multidoses of epigallocatechin gallate and coenzyme Q10 on cisplatin-induced oxidative stress in rat kidney.
|
The nephroprotective effect of coenzyme Q10 and epigallocatechin gallate was investigated in rats with acute renal injury induced by a single nephrotoxic dose of cisplatin. Two days prior to cisplatin administration, epigallocatechin gallate and coenzyme Q10 alone and in four different combinations were given for 6 days. The treatment with antioxidants significantly protected the cisplatin-induced increase in the levels of blood urea nitrogen and serum creatinine. Both the antioxidants alone or in different combinations significantly compensated the increased malondialdehyde and reduced glutathione levels. Moreover, the decrease in the activities of superoxide dismutase, catalase, and glutathione peroxidase and the concentration of selenium, zinc, and copper ions were significantly attenuated in renal tissue. In conclusion, epigallocatechin gallate and coenzyme Q10 are equally effective against cisplatin-induced nephrotoxicity, whereas the intervention by combining these two antioxidants was found to be highly effective at low doses in attenuating oxidative stress in rat kidney.
|
['Animals', 'Antineoplastic Agents', 'Antioxidants', 'Catechin', 'Cisplatin', 'Creatinine', 'Kidney', 'Kidney Diseases', 'Male', 'Micronutrients', 'Oxidative Stress', 'Oxidoreductases', 'Rats', 'Rats, Wistar', 'Ubiquinone', 'Urea']
| 25,382,014
|
[['B01.050'], ['D27.505.954.248'], ['D27.505.519.217', 'D27.505.696.706.125', 'D27.720.799.047'], ['D03.383.663.283.240.190', 'D03.383.663.283.266.450.206', 'D03.633.100.150.240.190', 'D03.633.100.150.266.450.206'], ['D01.210.375', 'D01.625.125', 'D01.710.100'], ['D03.383.129.308.207'], ['A05.810.453'], ['C12.777.419', 'C13.351.968.419'], ['D27.505.696.494', 'G07.203.300.681.500', 'J02.500.681.500'], ['G03.673', 'G07.775.750'], ['D08.811.682'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.900'], ['D02.806.250.900', 'D08.211.935'], ['D02.065.950']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Technology, Industry, and Agriculture [J]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Diagnostic performances of [18F]fluorocholine positron emission tomography in brain tumors.
|
BACKGROUND: Brain tumors characterization by molecular imaging that allows the depiction of brain lesions metabolic pattern is crucial. Our study aimed to: 1) to evaluate the diagnostic performances of [18F]fluoroethylcholine positron emission tomography/computed tomography ([18F]FECH PET/CT), and 2) correlate PET imaging derived parameters of [18F]FECH to survival in brain tumors.METHODS: From 2009 to 2012, we enrolled 30 patients who underwent [18F]FECH PET/CT. Final diagnosis was established by clinical and radiological follow-up.RESULTS: Final diagnosis was consistent with tumor disease in 27/30 cases. In 3/30 cases tumor disease was ruled out. [18F]FECH PET/CT resulted true positive and negative in 21/30 and 9/30 patients, respectively. Sensitivity, specificity, positive predictive value, negative predictive value and accuracy of [18F]FECH PET/CT were 78%, 100%, 100%, 33%, and 80%, respectively. Mean and maximum standardized uptake value (SUVmean and SUVmax) resulted statistically correlated to histology (P=0.0255 and P=0.0222, respectively). Using a SUVmax cut-off of 2.0 or 3.2, we distinguished between low- and high-grade gliomas with a good specificity (70% and 80%, respectively). SUVmax and histology resulted correlated to overall survival and disease related survival at multivariate analysis.CONCLUSIONS: Our results, worthy of further investigations, show high diagnostic performances of [18F]FECH PET/CT, and a correlation between PET imaging derived parameters and survival.
|
['Adult', 'Aged', 'Brain Neoplasms', 'Choline', 'Female', 'Fluorine Radioisotopes', 'Fluorodeoxyglucose F18', 'Humans', 'Male', 'Middle Aged', 'Positron Emission Tomography Computed Tomography', 'Radiopharmaceuticals', 'Sensitivity and Specificity']
| 26,329,494
|
[['M01.060.116'], ['M01.060.116.100'], ['C04.588.614.250.195', 'C10.228.140.211', 'C10.551.240.250'], ['D02.033.100.291.211', 'D02.092.063.291.211', 'D02.092.877.883.333', 'D02.675.276.232'], ['D01.496.749.340'], ['D09.254.229.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['E01.370.350.350.800.700.500', 'E01.370.350.350.810.645', 'E01.370.350.567.500', 'E01.370.350.600.350.700.810.490', 'E01.370.350.600.350.800.399.500', 'E01.370.350.700.700.810.645', 'E01.370.350.700.810.810.723', 'E01.370.350.710.800.399.500', 'E01.370.350.825.800.399.500', 'E01.370.350.825.810.810.700', 'E01.370.384.730.800.399.500'], ['D27.505.259.843', 'D27.505.519.871', 'D27.720.470.410.650'], ['E05.318.370.800', 'E05.318.740.872', 'G17.800', 'N05.715.360.325.700', 'N05.715.360.750.725', 'N06.850.520.445.800', 'N06.850.520.830.872']]
|
['Named Groups [M]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Circulating LncRNA Serve as Fingerprint for Gestational Diabetes Mellitus Associated with Risk of Macrosomia.
|
BACKGROUND/AIMS: Fetal macrosomia and its associated complications are the most frequent and serious morbidities for infants associated with gestational diabetes mellitus (GDM). The aim of this study was to evaluate the lncRNAs involvement in GDM, especially for the prediction of risk for fetal overgrowth.METHODS: The peripheral blood obtained from four group including healthy control (NC), healthy volunteers with pregnancy (NC-P), GDM patients with and without macrosomia were screened by lncRNA microarray and validated by quantitative real-time PCR (RT-qPCR) arranged in the training and a two-stage validation sets. The positive and negative prediction ability for candidate lncRNAs were analyzed by risk score analysis.RESULTS: A multiple venny analysis was performed revealed five candidate lncRNA including XLOC_014172, RP11-230G5.2, PCBP1-AS1, LOC149086 and RP11-160H22.5 which was consistence with the following parameter: i, increased in GDM patients with macrosomia (GDM-M) comparing with patients without macrosomia; ii, increased in GDM-M comparing with NC-P group; iii, increased in GDM-M comparing with NC. Further validation found XLOC_014172 and RP11-230G5.2 was final consistence with these parameter in 150 samples each group. Further receiver operating characteristic curve (ROC) analysis, with the combined two stably expressed lncRNAs indicated a high diagnostic ability an area under ROC curve value (AUC) of 0.955 and 0.962 in training set and validation set respectively.CONCLUSIONS: Circulating XLOC_014172 and RP11-230G5.2 may act as novel biomarkers in GDM patients as fingerprint for the risk of macrosomia outcome.
|
['Adult', 'Area Under Curve', 'Biomarkers', 'Case-Control Studies', 'Diabetes, Gestational', 'Female', 'Fetal Macrosomia', 'Glucose Tolerance Test', 'Glycated Hemoglobin A', 'Humans', 'Pregnancy', 'Pregnancy Complications', 'RNA, Long Noncoding', 'ROC Curve', 'Risk Factors']
| 30,036,868
|
[['M01.060.116'], ['E05.318.740.200', 'G03.787.101', 'G07.690.725.064', 'N06.850.520.830.200'], ['D23.101'], ['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['C13.703.170', 'C18.452.394.750.448', 'C19.246.200'], ['C13.703.170.500', 'C13.703.277.570', 'C13.703.726.570', 'C16.300.570', 'C19.246.099.968', 'C23.888.144.186.500'], ['E01.370.225.124.100.355', 'E01.370.374.355', 'E05.200.124.100.355'], ['D09.400.430.937', 'D12.776.124.400.405.440', 'D12.776.395.381', 'D12.776.422.316.762.380.440'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G08.686.784.769'], ['C13.703'], ['D13.444.735.790.375'], ['E05.318.370.800.750', 'E05.318.740.872.750', 'N05.715.360.325.700.680', 'N06.850.520.445.800.750'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Prevention of peritendinous adhesions with electrospun chitosan-grafted polycaprolactone nanofibrous membranes.
|
As one of the common complications after tendon injury and subsequent surgery, peritendinous adhesions could be minimized by directly placing a physical barrier between the injured site and the surrounding tissue. With the aim of solving the shortcomings of current biodegradable anti-adhesion barrier membranes, we propose the use of an electrospun chitosan-grafted polycaprolactone (PCL-g-CS) nanofibrous membrane (NFM) to prevent peritendinous adhesions. After introducing carboxyl groups on the surface by oxygen plasma treatment, the polycaprolactone (PCL) NFM was covalently grafted with chitosan (CS) molecules, with carbodiimide as the coupling agent. Compared with PCL NFM, PCL-g-CS NFM showed a similar fiber diameter, permeation coefficient for bovine serum albumin, ultimate tensile strain, reduced pore diameter, lower water contact angle, increased water sorption and tensile strength. With its submicrometer pore diameter (0.6-0.9ìm), both NFMs could allow the diffusion of nutrients and waste while blocking fibroblast penetration to prevent adhesion formation after tendon surgery. Cell culture experiments verified that PCL-g-CS NFM can reduce fibroblast attachment while maintaining the biocompatibility of PCL NFM, implicating a synergistic anti-adhesion effect to raise the anti-adhesion efficacy. In vivo studies with a rabbit flexor digitorum profundus tendon surgery model confirmed that PCL-g-CS NFM effectively reduced peritendinous adhesion from gross observation, histology, joint flexion angle, gliding excursion and biomechanical evaluation. An injured tendon wrapped with PCL-g-CS NFM showed the same tensile strength as the naturally healed tendon, indicating that the anti-adhesion NFM will not compromise tendon healing.
|
['Animals', 'Chitosan', 'Electroplating', 'Equipment Design', 'Equipment Failure Analysis', 'Guided Tissue Regeneration', 'Materials Testing', 'Membranes, Artificial', 'Nanofibers', 'Particle Size', 'Rabbits', 'Rotation', 'Tendinopathy', 'Tendons', 'Tensile Strength', 'Tissue Adhesions', 'Tissue Scaffolds', 'Treatment Outcome']
| 25,192,729
|
[['B01.050'], ['D05.750.078.139.500', 'D09.698.211.500'], ['E05.301.250.348'], ['E05.320'], ['E05.325.192'], ['E04.680.300'], ['E05.570'], ['D25.479', 'J01.637.051.479', 'J01.637.087.500'], ['J01.637.512.300'], ['G02.712'], ['B01.050.150.900.649.313.968.700'], ['G01.482.703'], ['C05.651.869', 'C26.874.800'], ['A02.880'], ['G01.374.850'], ['C23.550.355.274.840'], ['E07.206.627', 'E07.695.825'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Technology, Industry, and Agriculture [J]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Anatomy [A]', 'Health Care [N]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
|
Novel coronaviruses, astroviruses, adenoviruses and circoviruses in insectivorous bats from northern China.
|
Bats are considered as the reservoirs of several emerging infectious disease, and novel viruses are continually found in bats all around the world. Studies conducted in southern China found that bats carried a variety of viruses. However, few studies have been conducted on bats in northern China, which harbours a diversity of endemic insectivorous bats. It is important to understand the prevalence and diversity of viruses circulating in bats in northern China. In this study, a total of 145 insectivorous bats representing six species were collected from northern China and screened with degenerate primers for viruses belonging to six families, including coronaviruses, astroviruses, hantaviruses, paramyxoviruses, adenoviruses and circoviruses. Our study found that four of the viruses screened for were positive and the overall detection rates for astroviruses, coronaviruses, adenoviruses and circoviruses in bats were 21.4%, 15.9%, 20% and 37.2%, respectively. In addition, we found that bats in northern China harboured a diversity of novel viruses. Common Serotine (Eptesicus serotinu), Fringed long-footed Myotis (Myotis fimriatus) and Peking Myotis (Myotis pequinius) were investigated in China for the first time. Our study provided new information on the ecology and phylogeny of bat-borne viruses.
|
['Adenoviridae', 'Animals', 'Astroviridae', 'China', 'Chiroptera', 'Circoviridae', 'Coronaviridae', 'Phylogeny', 'Viruses']
| 28,371,451
|
[['B04.280.030'], ['B01.050'], ['B04.820.578.250'], ['Z01.252.474.164'], ['B01.050.150.900.649.313.937'], ['B04.280.120'], ['B04.820.578.500.540'], ['G05.697', 'G16.075.605', 'L01.100.697'], ['B04']]
|
['Organisms [B]', 'Geographicals [Z]', 'Phenomena and Processes [G]', 'Information Science [L]']
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 1
|
Excessive twitch movements in rapid eye movement sleep with daytime sleepiness.
|
A man who showed excessive twitch movement, such as fragmentary myoclonus (FM) and periodic movements in sleep (PMS) predominantly during REM sleep, is reported. He complained of excessive daytime sleepiness (EDS). After examination, his twitch movements were shown not to accompany narcolepsy, and his EDS were considered to originate from nocturnal sleep disturbance caused by FM and PMS.
|
['Adult', 'Humans', 'Male', 'Movement', 'Myoclonus', 'Sleep Stages', 'Sleep Wake Disorders', 'Sleep, REM']
| 9,472,125
|
[['M01.060.116'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G07.568', 'G11.427.410'], ['C10.597.350.500', 'C23.888.592.350.500'], ['F02.830.855.796', 'G11.561.803.754'], ['C10.886', 'C23.888.592.796', 'F03.870'], ['F02.830.855.796.671', 'G11.561.803.754.671']]
|
['Named Groups [M]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Psychiatry and Psychology [F]']
| 0
| 1
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Online shear viscosity measurement of starchy melts enriched in wheat bran.
|
UNLABELLED: Addition of wheat bran to flours modifies their expansion properties after cooking extrusion. This can be attributed to changes in the melt shear viscosity at the die. The effect of wheat bran concentration added to achieve 2 levels of dietary fibers of 12. 6% and 24.4%, and process conditions on the shear viscosity of wheat flour was therefore assessed using an online twin-slit rheometer. The shear viscosity measured at 30 s⁻? ranged from 9.5 ? 10³ to 53.4 ? 10³ Pa s. Regardless of the process conditions and bran concentration, the extruded melts showed a pseudoplastic behavior with a power law index n ranging from 0.05 to 0.27. Increasing the barrel temperature of the extruder from 120 to 180 °C, the water content from 18% to 22% or the screw speed from 400 to 800 rpm significantly decreased the melt shear viscosity at the extruder exit. The addition of bran significantly increased the melt shear viscosity only at the highest bran concentration. The effect was process condition dependant. Mathematical interpretations, based upon observations, of the experimental data were carried out. They can be used to predict the effect of the process conditions on the melt shear viscosity at the die of extruded wheat flour with increasing bran concentration. The viscosity data will be applied in future works to study the expansion properties of extruded wheat flour supplemented with bran.PRACTICAL APPLICATION: Incorporation of wheat bran, a readily available and low cost by-product, in extruded puffed foods is constrained due to its negative effect on the product texture. Understanding the effect of wheat bran on rheological properties of extruded melts, driving the final product properties, is essential to provide solutions to the food industry and enhance its use.
|
['Cooking', 'Dietary Fiber', 'Flour', 'Food Additives', 'Rheology', 'Starch', 'Temperature', 'Triticum', 'Viscosity', 'Water']
| 22,417,431
|
[['J01.576.423.200.200'], ['D09.301.416', 'G07.203.300.400', 'J02.500.400'], ['G07.203.300.484', 'J02.500.484'], ['D27.720.372.300', 'G07.203.300.514.500', 'J02.500.514.500'], ['E05.830', 'H01.671.808'], ['D05.750.078.562.855', 'D09.301.915', 'D09.698.365.855'], ['G01.906.595', 'G16.500.275.063.725.710', 'G16.500.750.775.710', 'N06.230.150.450', 'N06.230.300.100.725.710'], ['B01.650.940.800.575.912.250.822.918'], ['G02.930'], ['D01.045.250.875', 'D01.248.497.158.459.650', 'D01.650.550.925']]
|
['Technology, Industry, and Agriculture [J]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Health Care [N]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
|
[Body-packer & body-stuffer - a medical challenge].
|
Since the seventies, the practice of drug smuggling in the form of body packing has increased in the Western world. The goal of our study was to present an algorithm for the safe management of intracorporal drug transport based on clinical experience and current evidence. The retrospective study, conducted over the past four years in our hospital prison, analyzes and discusses the diagnostic and therapeutic concepts. Thirty-four patients hospitalized 37 times in a 48-month period were included. In 28 patients drug packages were identified. Only two patients suffered from serious complications. The study demonstrates that following a specifically designed management algorithm based on clinical experience and principles of evidence-based medicine can optimize risk management, improve quality assurance and patient safety.
|
['Adolescent', 'Adult', 'Algorithms', 'Analgesics, Opioid', 'Cocaine', 'Commerce', 'Crime', 'Drug and Narcotic Control', 'Electrocardiography', 'Female', 'Follow-Up Studies', 'Foreign Bodies', 'Gastrointestinal Agents', 'Hospitalization', 'Humans', 'Lactulose', 'Laxatives', 'Length of Stay', 'Magnetic Resonance Imaging', 'Male', 'Middle Aged', 'Poisoning', 'Radiography, Abdominal', 'Risk Factors', 'Time Factors', 'Tomography, X-Ray Computed', 'Transportation', 'Ultrasonography']
| 20,449,821
|
[['M01.060.057'], ['M01.060.116'], ['G17.035', 'L01.224.050'], ['D27.505.696.277.600.500', 'D27.505.696.663.850.014.760.500', 'D27.505.954.427.040.550.500', 'D27.505.954.427.210.600.500'], ['D02.145.074.722.388', 'D03.132.889.354', 'D03.605.084.500.722.388', 'D03.605.869.388'], ['J01.219'], ['I01.198.240', 'I01.880.735.191'], ['I01.880.604.605.250', 'N03.706.615.402.250', 'N04.452.706.310'], ['E01.370.370.380.240', 'E01.370.405.240'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['C26.392'], ['D27.505.954.483'], ['E02.760.400', 'N02.421.585.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D09.698.629.305.423', 'D09.947.750.423'], ['D27.505.954.483.620'], ['E02.760.400.480', 'N02.421.585.400.480'], ['E01.370.350.825.500'], ['M01.060.116.630'], ['C25.723'], ['E01.370.350.700.715'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['G01.910.857'], ['E01.370.350.350.810', 'E01.370.350.600.350.700.810', 'E01.370.350.700.700.810', 'E01.370.350.700.810.810', 'E01.370.350.825.810.810'], ['J01.937'], ['E01.370.350.850']]
|
['Named Groups [M]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Chemicals and Drugs [D]', 'Technology, Industry, and Agriculture [J]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 1
| 1
| 1
| 1
| 0
|
Zona-float method for separating mouse eggs from other cells.
|
We have developed a new method for separating mouse eggs from other cells, such as cumulus cells, using centrifugation with Percoll. Solutions of 45, 22.5, 11.3, and 5.6% Percoll were tested. With the 22.5% solution, 99% of whole eggs obtained by in vitro fertilization were collected from the upper part of the Percoll solution, and 98% of 2-cell embryos collected from these eggs developed to the blastocyst stage. Offspring were obtained after transfer of collected embryos to female mice. The greatest advantage of this method is that undamaged eggs are separated from other cells in one simple operation, regardless of the number of eggs.
|
['Animals', 'Cell Separation', 'Centrifugation', 'Embryo Transfer', 'Embryo, Mammalian', 'Female', 'Male', 'Mice', 'Ovum', 'Povidone', 'Silicon Dioxide']
| 15,297,709
|
[['B01.050'], ['E01.370.225.500.363', 'E05.200.500.363', 'E05.242.363'], ['E05.181'], ['E02.875.800.500', 'E05.820.800.500'], ['A16.254'], ['B01.050.150.900.649.313.992.635.505.500'], ['A05.360.490.690', 'A11.497.497', 'A16.690'], ['D02.455.326.271.884.533.699', 'D03.383.773.812.615', 'D05.750.716.721.838', 'D25.720.716.721.838', 'J01.637.051.720.716.721.838'], ['D01.578.750', 'D01.650.550.825', 'D01.837.725']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Technology, Industry, and Agriculture [J]']
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Alterations in vitamin A and E levels in liver and testis of wild ungulates from a lead mining area.
|
In animals, exposure to metal pollution can induce oxidative stress via several mechanisms. This stress might then cause adverse effects on functions such as male reproductive capacity. Antioxidant vitamins A and E play an important role in maintaining organism functions under stressed conditions. This study assessed the effect of different metals and metalloids on levels of vitamins A and E in livers and testis (n = 67 and 36) of red deer and in livers (n = 22) of wild boar. The study compared animals residing within and outside a polluted mining area. Red deer from mined areas showed significant reductions in liver retinyl docosahexaenoate and retinyl docosapentaenoate. Free retinol, á-tocopherol, and retinyl palmitate in the testis were also lower. This might indicate that increased internal usage of these antioxidants is occurring as deer try to maintain the integrity and function of reproductive tissue. Wild boar from mined areas also showed significant reductions in liver retinyl stearate but increased free retinol levels. This might suggest that vitamin A is being mobilized to a greater degree to cope with the induced oxidative stress caused by exposure to metal pollution. Additionally, a significant negative relationship between liver á-tocopherol and bone lead (Pb) in boar might indicate some long-term effects of Pb on antioxidant levels. Results suggest that vitamin A and E status can be altered as a consequence of exposure to Pb pollution and that complex differences in this response probably exist between species.
|
['Animals', 'Animals, Wild', 'Bone and Bones', 'Deer', 'Fatty Acids, Unsaturated', 'Lead', 'Liver', 'Male', 'Metalloids', 'Metals', 'Mining', 'Soil Pollutants', 'Spain', 'Sus scrofa', 'Testis', 'Vitamin A', 'Vitamin E']
| 20,857,095
|
[['B01.050'], ['B01.050.050.300'], ['A02.835.232', 'A10.165.265'], ['B01.050.150.900.649.313.500.380.373'], ['D10.251.355'], ['D01.268.556.435', 'D01.552.544.435'], ['A03.620'], ['D01.268.513'], ['D01.552'], ['J01.576.655.875.500'], ['D27.888.284.756'], ['Z01.542.846'], ['B01.050.150.900.649.313.500.880.399'], ['A05.360.444.849', 'A05.360.576.782', 'A06.300.312.782'], ['D02.455.326.271.665.202.495.818', 'D02.455.426.392.368.367.379.249.700.860', 'D02.455.849.131.495.818', 'D02.455.849.291.925', 'D23.767.261.700.860'], ['D03.383.663.283.909', 'D03.633.100.150.909']]
|
['Organisms [B]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Technology, Industry, and Agriculture [J]', 'Geographicals [Z]']
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
| 0
| 1
|
Risk factors of invasive Candida and non-Candida fungal infections after liver transplantation.
|
Fungal infections are associated with a high mortality rate after liver transplantation. To describe risk factors for fungal infections, 405 consecutive liver transplant recipients were analyzed. Forty-five patients (11%) developed invasive fungal infection. Median posttransplantation time to the first episode was 60 days. Pathogens were Candida species (spp) (n=24, 53%), Cryptococcus neoformans (n=10, 22%), Aspergillus spp (n=6, 13%), Rhizopus spp (n=l), and others (n=4). Presentations of infection included disseminated (n=9), intra-abdominal (n=9), esophageal (n=9), lung (n=8), blood (n=6), and central nervous system infections (n=3), and sinusitis with esophagitis (n=1). Eighteen patients (40%) with invasive fungal infection died, and 13 (72%) of these deaths were attributable to fungi. Mortality in the nonfungal infection group was 12%. Univariate analysis identified separate risk factors for Candida (intra-abdominal bleeding), Aspergillus (fulminant hepatitis), and cryptococcal (symptomatic cytomegalovirus infection) infections. In both univariate and multivariate analyses, a high intratransplant transfusion requirement and posttransplant bacterial infection were identified as significant risk factors for all types of fungal infection. The risk factor analysis reported here suggests that different pathogenic processes lead to Candida and non-Candida infection in liver transplant recipients. Their identification should prompt specific prophylactic measures to reduce morbidity and mortality in this population.
|
['Analysis of Variance', 'Candidiasis', 'Evaluation Studies as Topic', 'Humans', 'Liver Transplantation', 'Mycoses', 'Postoperative Complications', 'Risk Factors']
| 8,878,386
|
[['E05.318.740.150', 'N05.715.360.750.125', 'N06.850.520.830.150'], ['C01.150.703.160'], ['E05.337', 'N05.715.360.335'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.095.147.725.490', 'E04.210.650', 'E04.936.450.490', 'E04.936.580.490'], ['C01.150.703'], ['C23.550.767'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Diseases [C]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Quantification of febuxostat polymorphs using powder X-ray diffraction technique.
|
Febuxostat is a pharmaceutical compound with more than 20 polymorphs of which form A is most widely used and usually exists in a mixed polymorphic form with form G. In the present study, a quantification method for polymorphic form A and form G of febuxostat (FEB) has been developed using powder X-ray diffraction (PXRD). Prior to development of a quantification method, pure polymorphic form A and form G are characterized. A continuous scan with a scan rate of 3° min(-1) over an angular range of 3-40° 2è is applied for the construction of the calibration curve using the characteristic peaks of form A at 12.78° 2è (I/I0100%) and form G at 11.72° 2è (I/I0100%). The linear regression analysis data for the calibration plots shows good linear relationship with R(2)=0.9985 with respect to peak area in the concentration range 10-60 wt.%. The method is validated for precision, recovery and ruggedness. The limits of detection and quantitation are 1.5% and 4.6%, respectively. The obtained results prove that the method is repeatable, sensitive and accurate. The proposed developed PXRD method can be applied for the quantitative analysis of mixtures of febuxostat polymorphs (forms A and G).
|
['Calibration', 'Calorimetry, Differential Scanning', 'Febuxostat', 'Powder Diffraction', 'Thiazoles', 'X-Ray Diffraction']
| 25,636,167
|
[['E05.978.155'], ['E05.196.131.310', 'E05.196.370.310'], ['D02.886.675.274', 'D03.383.129.708.274'], ['E05.196.309.711'], ['D02.886.675', 'D03.383.129.708'], ['E05.196.309.742', 'E05.196.822.950', 'G01.867.950', 'G02.965']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Surface markers for guiding cylindrical diffuser fiber insertion in interstitial photodynamic therapy of head and neck cancer.
|
BACKGROUND AND OBJECTIVES: Image-based treatment planning can be used to compute the delivered light dose during interstitial photodynamic therapy (I-PDT) of locally advanced head and neck squamous cell carcinoma (LA-HNSCC). The objectives of this work were to evaluate the use of surface fiducial markers and flexible adhesive grids in guiding interstitial placement of laser fibers, and to quantify the impact of discrepancies in fiber location on the expected light dose volume histograms (DVHs).METHODS: Seven gel-based phantoms were made to mimic geometries of LA-HNSCC. Clinical flexible grids and fiducial markers were used to guide the insertion of optically transparent catheters, which are used to place cylindrical diffuser fibers within the phantoms. A computed tomography (CT) was used to image the markers and phantoms before and after catheter insertion and to determine the difference between the planned and actual location of the catheters. A finite element method was utilized to compute the light DVHs. Statistical analysis was employed to evaluate the accuracy of fiber placement and to investigate the correlation between the location of the fibers and the calculated DVHs.RESULTS: There was a statistically significant difference (P = 0.018) between all seven phantoms in terms of the mean displacement. There was also statistically significant correlation between DVHs and depth of insertion (P = 0.0027), but not with the lateral displacement (P = 0.3043). The maximum difference between actual and planned DVH was related to the number of fibers (P = 0.0025) and the treatment time.CONCLUSIONS: Surface markers and a flexible grid can be used to assist in the administration of a prescribed DVH within 15% of the target dose provided that the treatment fibers are placed within 1.3 cm of the planned depth of insertion in anatomies mimicking LA-HNSCC. The results suggest that the number of cylindrical diffuser fibers and treatment time can impact the delivered DVHs. Lasers Surg. Med. 49:599-608, 2017. © 2017 Wiley Periodicals, Inc.
|
['Antineoplastic Agents', 'Carcinoma, Squamous Cell', 'Catheterization', 'Fiducial Markers', 'Head and Neck Neoplasms', 'Humans', 'Models, Theoretical', 'Phantoms, Imaging', 'Photochemotherapy', 'Photosensitizing Agents', 'Squamous Cell Carcinoma of Head and Neck', 'Tomography, X-Ray Computed']
| 28,185,275
|
[['D27.505.954.248'], ['C04.557.470.200.400', 'C04.557.470.700.400'], ['E02.148', 'E05.157'], ['E05.978.808.249', 'E07.695.237', 'E07.710.259'], ['C04.588.443'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.599'], ['E07.671'], ['E02.186.500', 'E02.319.685', 'E02.774.722'], ['D27.505.954.444.600', 'D27.505.954.600.710'], ['C04.557.470.200.400.565', 'C04.588.443.177'], ['E01.370.350.350.810', 'E01.370.350.600.350.700.810', 'E01.370.350.700.700.810', 'E01.370.350.700.810.810', 'E01.370.350.825.810.810']]
|
['Chemicals and Drugs [D]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Tripartite organization of the ventral stream by animacy and object size.
|
Occipito-temporal cortex is known to house visual object representations, but the organization of the neural activation patterns along this cortex is still being discovered. Here we found a systematic, large-scale structure in the neural responses related to the interaction between two major cognitive dimensions of object representation: animacy and real-world size. Neural responses were measured with functional magnetic resonance imaging while human observers viewed images of big and small animals and big and small objects. We found that real-world size drives differential responses only in the object domain, not the animate domain, yielding a tripartite distinction in the space of object representation. Specifically, cortical zones with distinct response preferences for big objects, all animals, and small objects, are arranged in a spoked organization around the occipital pole, along a single ventromedial, to lateral, to dorsomedial axis. The preference zones are duplicated on the ventral and lateral surface of the brain. Such a duplication indicates that a yet unknown higher-order division of labor separates object processing into two substreams of the ventral visual pathway. Broadly, we suggest that these large-scale neural divisions reflect the major joints in the representational structure of objects and thus place informative constraints on the nature of the underlying cognitive architecture.
|
['Adolescent', 'Adult', 'Animals', 'Area Under Curve', 'Brain Mapping', 'Cerebral Cortex', 'Cognition', 'Face', 'Female', 'Humans', 'Image Processing, Computer-Assisted', 'Magnetic Resonance Imaging', 'Male', 'Mental Processes', 'Nerve Net', 'Photic Stimulation', 'Size Perception', 'Visual Pathways', 'Visual Perception', 'Young Adult']
| 23,785,139
|
[['M01.060.057'], ['M01.060.116'], ['B01.050'], ['E05.318.740.200', 'G03.787.101', 'G07.690.725.064', 'N06.850.520.830.200'], ['E01.370.350.578.875.500', 'E01.370.376.537.625.500', 'E05.629.875.500'], ['A08.186.211.200.885.287.500'], ['F02.463.188'], ['A01.456.505'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['L01.224.308'], ['E01.370.350.825.500'], ['F02.463'], ['A08.511'], ['E05.723.729'], ['F02.463.593.725', 'F02.463.593.778.794'], ['A08.612.220.860'], ['F02.463.593.932'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Anatomy [A]', 'Psychiatry and Psychology [F]', 'Information Science [L]']
| 1
| 1
| 0
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 1
| 1
| 1
| 0
|
Expression of acquired immunity to the hookworm Ancylostoma ceylanicum in hamsters.
|
Four experiments are described in which hamsters, initially exposed to primary infection with Ancylostoma ceylanicum, were given a homologous challenge and components of the secondary response were quantified and compared to relevant control groups. The initial establishment of the L3 larvae was not prevented in immunized hamsters but their growth was slowed and virtually all larvae were lost within a week of challenge, when the majority were still at the L4 stage of development. The loss of worms was associated with an accelerated mucosal mastocytosis and increased systemic antibody. Thus acquired immunity to hookworm larvae in this system acted on L3 and L4 stages, thereby preventing larvae from maturing in immunized animals. In contrast to primary infections, immunized hamsters responding to a challenge infection did not lose weight nor did they experience significant anaemia, because of the lack of adult worms. The secondary immune responses therefore prevented manifestation of hookworm disease among immunized-challenged animals.
|
['Ancylostoma', 'Ancylostomiasis', 'Animals', 'Antibodies, Helminth', 'Cricetinae', 'Female', 'Hematocrit', 'Immunity, Mucosal', 'Immunization', 'Immunization, Secondary', 'Larva', 'Male', 'Mast Cells', 'Weight Gain']
| 9,278,943
|
[['B01.050.500.500.294.400.968.100.100'], ['C01.610.335.508.700.775.455.154'], ['B01.050'], ['D12.776.124.486.485.114.185', 'D12.776.124.790.651.114.185', 'D12.776.377.715.548.114.185'], ['B01.050.150.900.649.313.992.635.075.250'], ['E01.370.225.625.400', 'E05.200.625.400', 'G09.188.370.374'], ['G12.450.573'], ['E02.095.465.425.400', 'E05.478.550', 'N02.421.726.758.310', 'N06.850.780.200.425', 'N06.850.780.680.310'], ['E02.095.465.425.400.485', 'E05.478.550.550'], ['B05.500.500', 'G07.345.500.550.500.500'], ['A11.329.427', 'A15.382.652'], ['C23.888.144.243.926', 'G07.345.249.314.120.200.926']]
|
['Organisms [B]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Biodiversity of algae and protozoa in a natural waste stabilization pond: a field study.
|
A field study was carried out on the biodiversity of protozoa and algae from a natural waste stabilization pond during November, 1996 to April, 1997. The raw waste and pond samples were analysed for physico-chemical and biological parameters. High dissolved oxygen (DO) coinciding with phytoplankton peak was recorded. The algae--Chlorella vulgaris, Scenedesmus acuminatus, Oscillatoria brevis and Nostoc piscinale and Protozoa--Paramecium caudatum, Acanthamoeba sp., Bodo saltans and Oikomonas termo were obvious as dominant species, whereas algae Ochromonas pyriformis and Synura uvella and protozoa, Didinium masutum and Stentor coerulus were noted as rare species. Totally 71 species of algae and 13 species of protozoa were identified.
|
['Animals', 'Catalase', 'Eukaryota', 'Oxygen', 'Population Dynamics', 'Waste Disposal, Fluid', 'Water Microbiology']
| 12,974,463
|
[['B01.050'], ['D08.811.682.732.332'], ['B01'], ['D01.268.185.550', 'D01.362.670'], ['I01.240.600', 'N01.224.625', 'N06.850.505.400.700'], ['N06.850.780.200.800.800.890', 'N06.850.860.510.900.600.900'], ['H01.158.273.540.274.777', 'N06.850.425.450']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]', 'Disciplines and Occupations [H]']
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 1
| 0
|
Motor unit firing in amyotrophic lateral sclerosis and other upper and lower motor neurone disorders.
|
OBJECTIVE: Motor unit recruitment order and firing rate was investigated in healthy subjects, and in small numbers of patients 50years ago. We aimed to investigate firing rate in different disorders, testing the same target muscle with normal strength, to evaluate possible application in diagnosing upper motor neuron (UMN) lesion.METHODS: We studied motor unit firing in the tibialis anterior muscle in six groups of subjects; normal subjects (n=45), patients with amyotrophic lateral sclerosis (ALS) (n=36), primary lateral sclerosis (PLS) (n=21), progressive muscular atrophy (PMA) (n=14), various upper motor neurone lesions (n=16) and polyneuropathy (n=42). In all these subjects the tibialis anterior muscle was of normal strength. Motor units were recruited during slight contraction in order to study 2-5 motor units at each recording site, using a standard concentric needle electrode, so that 20-22 motor units were recorded in each muscle. We analysed the coefficient of variation (CV) for amplitude, area, duration and firing rate in these motor units, and the correlation between motor unit potential size and recruitment order.RESULTS: The mean MU firing rate in this task was similar in each group. No recruitment order was disclosed within the limits of the study task. The CV of firing rate was decreased in UMN and PLS groups. ALS patients with marked spasticity showed a lower CV of motor unit firing rate. The CV of amplitude, area and duration was similar between groups.CONCLUSIONS: These results in tibialis anterior indicate that physiological modulation of lower motor neuron (LMN) firing rate is decreased in patients with lower limb spasticity. The variability of MU discharges tends to be greater in diseases affecting the LMN.SIGNIFICANCE: These results suggest that, notwithstanding the simplicity of the task we have used, the physiological variability of motor unit firing may be a useful variable in assessing UMN involvement in motor system disorders.
|
['Adult', 'Aged', 'Aged, 80 and over', 'Amyotrophic Lateral Sclerosis', 'Case-Control Studies', 'Electromyography', 'Female', 'Humans', 'Male', 'Middle Aged', 'Motor Neuron Disease', 'Motor Neurons', 'Muscle, Skeletal', 'Muscular Atrophy, Spinal', 'Polyneuropathies', 'Recruitment, Neurophysiological']
| 22,627,021
|
[['M01.060.116'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['C10.228.854.139', 'C10.574.562.250', 'C10.574.950.050', 'C10.668.467.250', 'C18.452.845.800.050'], ['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['E01.370.405.255', 'E01.370.530.255'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['C10.574.562', 'C10.668.467'], ['A08.675.655.500', 'A11.671.655.500'], ['A02.633.567', 'A10.690.552.500'], ['C10.228.854.468', 'C10.574.562.500', 'C10.668.467.500'], ['C10.668.829.800'], ['G07.265.753.760', 'G11.561.601.760']]
|
['Named Groups [M]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Anatomy [A]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Study on the influence of oxidative stress on the fibrillization of fibrinogen.
|
Human fibrinogen is an important coagulation factor as well as an independent predictor of coronary heart disease and stroke. Analysis of dysfibrinogens may provide useful information and help us to understand the molecular defects in fibrin polymerization. In the present study, we investigated the influence of oxidative stress of fibrinogen induced by H2O2 on the polymerization state of fibrin. UV absorbance spectroscopy, circular dichroism, æ-potential, dynamic light scattering and steady shear viscosity were all employed to study the influence of oxidative stress on the molecular structure, the surface charges, and the size and shape of fibrinogen molecules. The fibrin morphology obtained was imaged and investigated using atomic force microscopy. The results demonstrated that the cross-linking, branching and height distribution of formed fibrin will be influenced by the oxidative stress of fibrinogen. This study presents new insights into the aggregation behaviour of fibrinogen and will be helpful to understand the formation mechanism of thrombosis under oxidative stress.
|
['Circular Dichroism', 'Fibrin', 'Fibrinogen', 'Humans', 'Hydrogen Peroxide', 'Microscopy, Atomic Force', 'Oxidative Stress', 'Spectrometry, Fluorescence', 'Spectrophotometry, Ultraviolet']
| 27,702,872
|
[['E05.196.867.151'], ['D12.776.124.270'], ['D12.776.124.050.250', 'D12.776.124.125.500', 'D12.776.811.300', 'D23.119.490'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D01.248.497.158.685.750.424', 'D01.339.431.374.424', 'D01.650.550.750.400', 'D02.389.338.253'], ['E01.370.350.515.666.400', 'E05.595.666.400'], ['G03.673', 'G07.775.750'], ['E05.196.712.516.600.676', 'E05.196.867.726'], ['E05.196.712.726.802', 'E05.196.867.826.802']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Fetal weight estimation in diabetic pregnancies using the gestation-adjusted projection method: comparison of two timing strategies for third-trimester sonography.
|
OBJECTIVES: The gestation-adjusted projection method extrapolates birth weight using third-trimester sonography. This technique is shown to be more accurate for sonographic examinations from 34 weeks to 36 weeks 6 days than 37 weeks to 38 weeks 6 days. Our objective was to determine whether even earlier sonographic examinations (31 weeks-33 weeks 6 days) further improves birth weight prediction in patients with diabetes.METHODS: We conducted a retrospective cohort analysis of 388 pregnant women with pregestational or gestational diabetes who delivered at 37 weeks or later and had a sonographic examination performed between 31 weeks and 36 weeks 6 days. Sonographic examinations were categorized as "early" if performed at 31 weeks to 33 weeks 6 days or "late" if performed at 34 weeks to 36 weeks 6 days. We estimated birth weight using the gestation-adjusted projection method, compared errors in prediction of birth weight using the t test and Mann-Whitney U test, and performed a 2-sample test of proportions to compare prediction of macrosomia (birth weight >4000 g).RESULTS: The early and late groups had similar mean gestational ages at birth (38 weeks 4 days versus 38 weeks 5 days; P = .13) and rates of macrosomia (10.7% versus 12.4%; P = .63). The early group had a greater mean absolute error (336 versus 297 g; P = .03) and percent error (9.9% versus 7.9%; P = .01) in birth weight prediction but a lower mean birth weight (3303 versus 3426 g; P = .02). Sensitivity for prediction of macrosomia was 19% in the early group versus 45% in the late group (P = .07), whereas specificity was similar (98% versus 96%; P = .27).CONCLUSIONS: Using the gestation-adjusted projection method in our patients with diabetes, we found that sonographic examinations performed at 34 weeks to 36 weeks 6 days better predicted birth weight than those performed at 31 weeks to 33 weeks 6 days.
|
['Adult', 'Birth Weight', 'Cohort Studies', 'Diabetes, Gestational', 'Female', 'Fetal Weight', 'Humans', 'Pregnancy', 'Pregnancy Complications', 'Pregnancy Trimester, Third', 'Retrospective Studies', 'Time Factors', 'Ultrasonography, Prenatal']
| 26,014,315
|
[['M01.060.116'], ['C23.888.144.186', 'E01.370.600.115.100.160.120.186', 'E05.041.124.160.750.149', 'G07.100.100.160.120.186', 'G07.345.249.314.120.186'], ['E05.318.372.500.750', 'N05.715.360.330.500.750', 'N06.850.520.450.500.750'], ['C13.703.170', 'C18.452.394.750.448', 'C19.246.200'], ['C23.888.144.300', 'E01.370.600.115.100.160.120.300', 'E05.041.124.160.750.300', 'G07.100.100.160.120.300', 'G07.345.249.314.120.300', 'G07.345.500.325.235.937', 'G08.686.280'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G08.686.784.769'], ['C13.703'], ['G08.686.707.520'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['G01.910.857'], ['E01.370.350.850.865', 'E01.370.378.630.865']]
|
['Named Groups [M]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
New insights on the use of desipramine as an inhibitor for acid ceramidase.
|
Treatment of different cancer cell lines with desipramine induced a time- and dose-dependent downregulation of acid ceramidase. Desipramine's effect on acid ceramidase appeared specific for amphiphilic agents (desipramine, chlorpromazine, and chloroquine) but not other lysomotropic agents such as ammonium chloride and bafilomycin A1, and was not transcriptionally regulated. The cathepsin B/L inhibitor, CA074ME, but not the cathepsin D inhibitor, pepstatin A, blocked desipramine's effect on acid ceramidase. Desipramine led to a more pronounced downregulation of sphingosine compared to ceramide suggesting acid ceramidase inhibition is important to desipramine's mechanism of action. This study reveals a new mechanism of action for desipramine.
|
['Antidepressive Agents, Tricyclic', 'Cell Line, Tumor', 'Cysteine Endopeptidases', 'Desipramine', 'Enzyme Inhibitors', 'Galactosylgalactosylglucosylceramidase', 'Humans', 'Hydrolysis', 'Male', 'Reverse Transcriptase Polymerase Chain Reaction', 'Sphingolipids']
| 16,901,483
|
[['D27.505.954.427.700.122.055'], ['A11.251.210.190', 'A11.251.860.180'], ['D08.811.277.656.262.500', 'D08.811.277.656.300.200'], ['D03.633.300.240.281'], ['D27.505.519.389'], ['D08.811.277.450.410.150'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G02.380'], ['E05.393.620.500.725'], ['D10.570.877']]
|
['Chemicals and Drugs [D]', 'Anatomy [A]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[HIV/AIDS and middle aged: knowledge assessment of people from Vale do Sinos, Rio Grande do Sul State, Brazil].
|
The objective of this article is to assess the knowledge about HIV/aids in middle aged acquaintance group participants from the Vale do Sinos, Rio Grande do Sul State, Brazil. A prospective cross-sectional study with 168 individuals (9.5% male and 90.5% female) between 40 and 59 years of age was performed. A questionnaire comprising questions about the concept, transmission, vulnerability, prevention, and treatment domains on HIV issues was constructed. It was observed that 61.3% of the sample had finished elementary education and 45.2% had a monthly income ranging from 1 to 3 minimum wages. In the conceptual domain, 65.2% did not know that HIV infection has an asymptomatic phase and 34.5% believed HIV could be transmitted by a mosquito bite. In prevention and vulnerability domains, 19.5% had no knowledge about female condoms and 29.2% believed that the disease was confined to specific groups. Regarding to antiretroviral treatment, 12.5% had no idea about its existence. According to findings, middle aged participants from acquaintance groups in Vale do Sinos had misconceptions about HIV/aids which might enhance the risk of infection. Therefore it is necessary to provide public health programs directed to this population group in order to prevent or decrease the risk of HIV transmission.
|
['Acquired Immunodeficiency Syndrome', 'Adult', 'Brazil', 'Cross-Sectional Studies', 'Female', 'HIV Infections', 'Health Knowledge, Attitudes, Practice', 'Humans', 'Male', 'Middle Aged', 'Prospective Studies', 'Surveys and Questionnaires']
| 20,640,277
|
[['C01.221.250.875.040', 'C01.221.812.640.400.040', 'C01.778.640.400.040', 'C01.925.782.815.616.400.040', 'C01.925.813.400.040', 'C01.925.839.040', 'C20.673.480.040'], ['M01.060.116'], ['Z01.107.757.176'], ['E05.318.372.500.875', 'N05.715.360.330.500.875', 'N06.850.520.450.500.875'], ['C01.221.250.875', 'C01.221.812.640.400', 'C01.778.640.400', 'C01.925.782.815.616.400', 'C01.925.813.400', 'C20.673.480'], ['F01.100.150.500', 'N05.300.150.410'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['E05.318.308.980', 'N05.715.360.300.800', 'N06.850.520.308.980']]
|
['Diseases [C]', 'Named Groups [M]', 'Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Psychiatry and Psychology [F]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Effect of age and race/ethnicity on HbA1c levels in people without known diabetes mellitus: implications for the diagnosis of diabetes.
|
AIMS: To determine if age and race/ethnicity affect HbA1c levels independent of glycemia.METHODS: We analyzed 2712 individuals from the NHANES III population 40-74 years old without diabetes history.RESULTS: HbA1c levels increased by 0.10% per decade in people with NGT and 0.07% in those with IFG and/or IGT, independent of fasting and 2-h glucose on OGTT's. Compared to non-Hispanic whites, HbA1c levels increased by 0.12% (NGT) and 0.10% (IFG/IGT) in Mexican-Americans and 0.21% (NGT) and 0.35% (IFG/IGT) in non-Hispanic blacks, independent of glycemia. At HbA1c levels of >or=6.5%, >or=7.0% and 6.5-6.9%, diabetes diagnosed by current FPG/OGTT criteria occurred in 82%, 94% and 65%, respectively. In non-Hispanic blacks with HbA1c levels of 6.5-6.9%, 68% of those 40-74 years old and 87% of those over 64 years old would not have diabetes by FPG/OGTT criteria. Over 90% of all race/ethnicity groups would have diabetes with HbA1c levels >or=7.0%.CONCLUSIONS: Because many people, especially older non-Hispanic blacks, with HbA1c levels of 6.5-6.9% would not have diabetes by current FPG/OGTT criteria and clinical retinopathy and nephropathy are very unusual in patients whose HbA1c levels are kept <7.0%; we recommend an HbA1c level of >or=7.0% to diagnose diabetes.
|
['Adult', 'African Americans', 'Age Factors', 'Aged', 'Continental Population Groups', 'Diabetes Mellitus', 'European Continental Ancestry Group', 'Female', 'Glucose Tolerance Test', 'Glycated Hemoglobin A', 'Health Surveys', 'Hispanic Americans', 'Humans', 'Male', 'Middle Aged', 'Population Surveillance', 'Regression Analysis', 'Sex Factors']
| 20,061,043
|
[['M01.060.116'], ['M01.686.508.100.100', 'M01.686.754.100'], ['N05.715.350.075', 'N06.850.490.250'], ['M01.060.116.100'], ['M01.686.508'], ['C18.452.394.750', 'C19.246'], ['M01.686.508.400'], ['E01.370.225.124.100.355', 'E01.370.374.355', 'E05.200.124.100.355'], ['D09.400.430.937', 'D12.776.124.400.405.440', 'D12.776.395.381', 'D12.776.422.316.762.380.440'], ['E05.318.308.980.438', 'N05.715.360.300.800.438', 'N06.850.520.308.980.438'], ['M01.686.754.441'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['E05.318.308.980.438.700', 'N05.715.360.300.800.438.625', 'N06.850.520.308.980.438.700', 'N06.850.780.675'], ['E05.318.740.750', 'N05.715.360.750.695', 'N06.850.520.830.750'], ['N05.715.350.675', 'N06.850.490.875']]
|
['Named Groups [M]', 'Health Care [N]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
A code of ethics for European health librarians: the points of departure.
|
Codes of ethics are a classic mark of a profession, and their preparation is an important part of the work expected of a professional organization. EAHIL has recently embarked on drafting a code for European health librarians. This paper explains the background to EAHIL's decision, and reviews existing codes of ethical practice in the fields of both medicine and library and information work.
|
['Codes of Ethics', 'England', 'Ethics, Professional', 'Europe', 'Information Dissemination', 'Internationality', 'Libraries, Medical', 'Library Associations']
| 10,151,526
|
[['K01.752.566.479.068', 'K01.752.566.479.171.066', 'N04.761.700.350.324', 'N05.350.213', 'N05.350.340.080', 'N05.700.350.324'], ['Z01.542.363.300'], ['K01.752.566.479.171', 'N05.350.340'], ['Z01.542'], ['L01.143.443'], ['I01.615'], ['J03.400.596.831.937', 'L01.346.596.719.875'], ['L01.583.390']]
|
['Humanities [K]', 'Health Care [N]', 'Geographicals [Z]', 'Information Science [L]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Technology, Industry, and Agriculture [J]']
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 1
| 0
| 1
| 1
|
The phenology of Rubus fruticosus in Ireland: herbarium specimens provide evidence for the response of phenophases to temperature, with implications for climate warming.
|
To date, phenological research has provided evidence that climate warming is impacting both animals and plants, evidenced by the altered timing of phenophases. Much of the evidence supporting these findings has been provided by analysis of historic records and present-day fieldwork; herbaria have been identified recently as an alternative source of phenological data. Here, we used Rubus specimens to evaluate herbaria as potential sources of phenological data for use in climate change research and to develop the methodology for using herbaria specimens in phenological studies. Data relevant to phenology (collection date) were recorded from the information cards of over 600 herbarium specimens at Ireland's National Herbarium in Dublin. Each specimen was assigned a score (0-5) corresponding to its phenophase. Temperature data for the study period (1852 - 2007) were obtained from the University of East Anglia's Climate Research Unit (CRU); relationships between temperature and the dates of first flower, full flower, first fruit and full fruit were assessed using weighted linear regression. Of the five species of Rubus examined in this study, specimens of only one (R. fruticosus) were sufficiently abundant to yield statistically significant relationships with temperature. The results revealed a trend towards earlier dates of first flower, full flower and first fruit phenophases with increasing temperature. Through its multi-phenophase approach, this research serves to extend the most recent work-which validated the use of herbaria through use of a single phenophase-to confirm herbarium-based research as a robust methodology for use in future phenological studies.
|
['Climate Change', 'Flowers', 'Fruit', 'History, 19th Century', 'History, 20th Century', 'History, 21st Century', 'Ireland', 'Periodicity', 'Rosaceae', 'Temperature']
| 22,382,508
|
[['G16.500.175.374'], ['A18.024.249.500'], ['A18.024.500', 'G07.203.300.562', 'J02.500.562'], ['K01.400.504.937'], ['K01.400.504.968'], ['K01.400.504.984'], ['Z01.542.467', 'Z01.639.587'], ['G01.910.645', 'G07.180.562'], ['B01.650.940.800.575.912.250.859.937.500'], ['G01.906.595', 'G16.500.275.063.725.710', 'G16.500.750.775.710', 'N06.230.150.450', 'N06.230.300.100.725.710']]
|
['Phenomena and Processes [G]', 'Anatomy [A]', 'Technology, Industry, and Agriculture [J]', 'Humanities [K]', 'Geographicals [Z]', 'Organisms [B]', 'Health Care [N]']
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
|
Robust optimization in IMPT using quadratic objective functions to account for the minimum MU constraint.
|
PURPOSE: Currently, in clinical practice of intensity-modulated proton therapy (IMPT), the influence of the minimum monitor unit (MU) constraint is taken into account through postprocessing after the optimization is completed. This may degrade the plan quality and plan robustness. This study aims to mitigate the impact of the minimum MU constraint directly during the plan robust optimization.METHODS AND MATERIALS: Cao et al. have demonstrated a two-stage method to account for the minimum MU constraint using linear programming without the impact of uncertainties considered. In this study, we took the minimum MU constraint into consideration using quadratic optimization and simultaneously had the impact of uncertainties considered using robust optimization. We evaluated our method using seven cancer patients with different machine settings.RESULT: The new method achieved better plan quality than the conventional method. The D95% of the clinical target volume (CTV) normalized to the prescription dose was (mean [min-max]): (99.4% [99.2%-99.6%]) vs. (99.2% [98.6%-99.6%]). Plan robustness derived from these two methods was comparable. For all seven patients, the CTV dose-volume histogram band gap (narrower band gap means more robust plans) at D95% normalized to the prescription dose was (mean [min-max]): (1.5% [0.5%-4.3%]) vs. (1.2% [0.6%-3.8%]).CONCLUSION: Our new method of incorporating the minimum MU constraint directly into the plan robust optimization can produce machine-deliverable plans with better tumor coverage while maintaining high-plan robustness.
|
['Algorithms', 'Head and Neck Neoplasms', 'Humans', 'Lung Neoplasms', 'Male', 'Prostatic Neoplasms', 'Radiotherapy Dosage', 'Radiotherapy Planning, Computer-Assisted', 'Radiotherapy, Intensity-Modulated', 'Retrospective Studies', 'Time Factors', 'Tomography, X-Ray Computed']
| 29,148,570
|
[['G17.035', 'L01.224.050'], ['C04.588.443'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C04.588.894.797.520', 'C08.381.540', 'C08.785.520'], ['C04.588.945.440.770', 'C12.294.260.750', 'C12.294.565.625', 'C12.758.409.750'], ['E02.815.639'], ['E02.950.825', 'L01.313.500.750.100.710.600.608'], ['E02.815.635.700.700', 'L01.313.500.750.100.710.600.550.700'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['G01.910.857'], ['E01.370.350.350.810', 'E01.370.350.600.350.700.810', 'E01.370.350.700.700.810', 'E01.370.350.700.810.810', 'E01.370.350.825.810.810']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Diseases [C]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 1
| 0
|
A system of safety management practices and worker engagement for reducing and preventing accidents: an empirical and theoretical investigation.
|
OBJECTIVE: The overall research objective was to theoretically and empirically develop the ideas around a system of safety management practices (ten practices were elaborated), to test their relationship with objective safety statistics (such as accident rates), and to explore how these practices work to achieve positive safety results (accident prevention) through worker engagement.METHOD: Data were collected using safety manager, supervisor and employee surveys designed to assess and link safety management system practices, employee perceptions resulting from existing practices, and safety performance outcomes.RESULTS: Results indicate the following: there is a significant negative relationship between the presence of ten individual safety management practices, as well as the composite of these practices, with accident rates; there is a significant negative relationship between the level of safety-focused worker emotional and cognitive engagement with accident rates; safety management systems and worker engagement levels can be used individually to predict accident rates; safety management systems can be used to predict worker engagement levels; and worker engagement levels act as mediators between the safety management system and safety performance outcomes (such as accident rates).IMPLICATIONS: Even though the presence of safety management system practices is linked with incident reduction and may represent a necessary first-step in accident prevention, safety performance may also depend on mediation by safety-focused cognitive and emotional engagement by workers. Thus, when organizations invest in a safety management system approach to reducing/preventing accidents and improving safety performance, they should also be concerned about winning over the minds and hearts of their workers through human performance-based safety management systems designed to promote and enhance worker engagement.
|
['Accident Prevention', 'Accidents, Occupational', 'Humans', 'Models, Organizational', 'Models, Theoretical', 'Organizational Objectives', 'Safety Management']
| 23,993,683
|
[['N06.850.135.060'], ['N06.850.135.240'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.599.670', 'N04.452.534'], ['E05.599'], ['N04.452.615'], ['N04.452.871.900', 'N06.850.135.060.075.800']]
|
['Health Care [N]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Association of adiposity, dysmetabolisms, and inflammation with aggressive breast cancer subtypes: a cross-sectional study.
|
Obesity and metabolic syndrome are risk and prognostic factors for breast cancer (BC) and are associated with chronic inflammation. We investigated the association between distinct BC subtypes and markers of adiposity, dysmetabolisms, and inflammation. We analyzed 1779 patients with primary invasive BC treated at a single institution, for whom anthropometric and clinical-pathological data were archived. BC subtypes were classified by immunohistochemical staining of ER, PR, HER2, and Ki67, and their relations with the study markers were assessed by multinomial logistic regression. Adjusted odds ratios (ORs) and 95 % confidence intervals (CIs) were calculated taking luminal A as reference. All subtypes more aggressive than luminal A were significantly more frequent in younger (<45 years) than older women. Before menopause, luminal B HER2-negative tumors were positively associated with large waist (OR 2.55, 95 % CI 1.53-4.24) and insulin resistance (OR 1.90, 95 % CI 1.05-3.41); luminal B HER2-positive tumors with large waist (OR 2.11, 95 % CI 1.03-4.35) and triple-negative tumors with overweight (OR 3.04, 95 % CI 1.43-6.43) and high C-reactive protein (p trend = 0.026). In postmenopausal women aged <65, luminal B HER2-negative (OR 1.94, 95 % CI 1.16-3.24) and luminal B HER2-positive tumors (OR 2.48, 95 % CI 1.16-5.27) were positively related with metabolic syndrome. Dysmetabolisms and inflammation may be related to different BC subtypes. Before menopause, triple-negative cancers were related to obesity and chronic inflammation, and aggressive luminal subtypes to abdominal adiposity. After menopause, in women aged <65 these latter subtypes were related to metabolic syndrome. Control of adiposity and dysmetabolism can reduce the risk of aggressive BC subtypes, improving the prognosis.
|
['Adult', 'Aged', 'Biomarkers, Tumor', 'Breast Neoplasms', 'C-Reactive Protein', 'Cross-Sectional Studies', 'Female', 'Humans', 'Insulin Resistance', 'Metabolic Syndrome', 'Middle Aged', 'Obesity', 'Odds Ratio', 'Waist Circumference']
| 27,117,160
|
[['M01.060.116'], ['M01.060.116.100'], ['D23.101.140'], ['C04.588.180', 'C17.800.090.500'], ['D12.776.034.145', 'D12.776.124.050.120', 'D12.776.124.486.157'], ['E05.318.372.500.875', 'N05.715.360.330.500.875', 'N06.850.520.450.500.875'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C18.452.394.968.500', 'G07.690.773.984.617'], ['C18.452.394.968.500.570', 'C18.452.625'], ['M01.060.116.630'], ['C18.654.726.500', 'C23.888.144.699.500', 'E01.370.600.115.100.160.120.699.500', 'G07.100.100.160.120.699.500'], ['E05.318.740.600.600', 'G17.680.500', 'N05.715.360.750.625.590', 'N06.850.520.830.600.600'], ['E01.370.600.115.100.160.560', 'E05.041.124.160.875', 'G07.100.100.160.560']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
[Analysis of risk factors for triplet pregnancy after a simultaneous transfer of triplicate embryos].
|
OBJECTIVE: To analyze the risk factors for triplet pregnancy after a simultaneous transfer of triplicate embryos.METHODS: The investigators carried out a retrospective analysis of 769 cycles in which three embryos were transferred in one treatment cycle, including 298 fresh embryo transfer (ET) cycles and 471 frozen-thawed ET (FET) cycles. The impact of patient age and the number of good embryos transferred on the rates of clinical pregnancy and triplet pregnancy was studied according to different cycle types.RESULTS: (1) The rates of clinical and triplet pregnancy were significantly higher in the FET group (P < 0.05) than those in the fresh ET group; (2) all patients with a triplet pregnancy in the fresh ET group (n = 6) were younger than 35 years old (P < 0.01). There was no significant difference between the subgroups in the FET cycle according to patient age (P > 0.05); (3) when none, 1, 2 or 3 good embryos were transferred in the fresh ET cycle, the clinical pregnancy rates were 28.3%, 46.7%, 50.6% and 58.7% and the triplet pregnancy rates 0, 2.3%, 4.7% and 6.8% respectively. A similar clinical pregnancy rate (P > 0.05) and a significantly lower triplet pregnancy rate (P < 0.05) were observed when 1 good embryo was transferred versus 2 good embryos (P < 0.05). When 0, 1, 2 or 3 good embryos were transferred in the FET cycle, the clinical pregnancy rates were 38.9%, 54.8%, 59.7%, 63.9% and the triplet pregnancy rates 0, 5.0%, 13.8%, 15.8% respectively. A similar clinical pregnancy rate (P > 0.05) and a significantly lower triplet pregnancy rate (P < 0.05) were observed when 1 good embryo was transferred versus two good embryos (P < 0.05). All triplet pregnancies occurred in cycles in which more than 1 good embryo was transferred (P < 0.05).CONCLUSION: The patients have more triplet pregnancies in the FET cycle than in the fresh ET cycle. In the FET cycle, the patient age is irrelevant. It is recommended that no more than 2 embryos should be transferred. Selective single blastocyst embryo transfer is preferable if there are more than 2 good embryos available for transfer. No more than 2 embryos should be transferred in the fresh ET cycle if good embryos are available and a patient is under 35 years old.
|
['Adult', 'Embryo Transfer', 'Female', 'Humans', 'Maternal Age', 'Pregnancy', 'Pregnancy Rate', 'Retrospective Studies', 'Risk Factors', 'Triplets', 'Young Adult']
| 21,418,975
|
[['M01.060.116'], ['E02.875.800.500', 'E05.820.800.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G08.686.560', 'N05.715.350.075.550', 'N06.850.490.250.550'], ['G08.686.784.769'], ['E05.318.308.985.775', 'G08.686.705', 'N01.224.935.849', 'N06.850.505.400.975.775', 'N06.850.520.308.985.775'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['M01.438.768'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Health Care [N]']
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Intracisternal administration of NR2 antagonists attenuates facial formalin-induced nociceptive behavior in rats.
|
AIMS: To examine the antinociceptive effects of N-Methyl-D-aspartate (NMDA) receptor NR2 subunit antagonists in a rat model of the facial formalin test.METHODS: Experiments were carried out on adult male Sprague-Dawley rats weighing 220 to 280 g. Anesthetized rats were individually mounted on a stereotaxic frame and a polyethylene tube was implanted for intracisternal injection and, 72 hours later, formalin tests were performed. NMDA receptor antagonists were administered intracisternally 10 minutes prior to subcutaneous injection of 5% formalin (50 MicroL) into the vibrissal pad.RESULTS: The intracisternal administration of 25, 50, or 100 Microg of memantine, an antagonist that acts at the NMDA ion channel site, significantly suppressed the number of scratches in the second phase of the behavioral responses to formalin. Intracisternal administration of a range of doses of 5,7-dichlorokynurenic acid, a glycine site antagonist, or DL-2-amino-5-phosphonopentanoate (AP-5), a nonselective NMDA site antagonist, produced significant antinociceptive effects in the second phase. Intracisternal administration of 1, 2.5, or 5 Microg of (2R,4S)-4-(3 Phosphonopropyl)-2-piperidine_carboxylic acid (PPPA), a competitive NR2A antagonist, significantly suppressed the number of scratches in the second phase, while only the highest dose of PPPA (5 Microg) significantly suppressed the number of scratches in the first phase. The antinociceptive effects of intracisternal injection of (alphaR, betaS)-alpha-(4Hydroxyphenyl)-_ methyl-4-(phenylmethyl)-1-Piperidinepropanol maleate(Ro 25-6981), a selective NR2B antagonist, were similar to those of PPPA. Injection of memantine, AP-5, Ro 25-6981, or vehicle did not result in any motor dysfunction. A low dose of PPPA (1 microg) or 5,7-dichlorokynurenic acid (2.5 microg) did not affect motor function. However, higher doses of PPPA and 5,7-dichlorokynurenic acid produced motor dysfunction.CONCLUSION: The present results suggest that central NR2 subunits play an important role in orofacial nociceptive transmission. Moreover, this data also indicate that targeted inhibition of the NMDA receptor NR2 subunit is a potentially important new treatment approach for inflammatory pain originating in the orofacial area.
|
['Animals', 'Behavior, Animal', 'Cisterna Magna', 'Disease Models, Animal', 'Excitatory Amino Acid Antagonists', 'Facial Pain', 'Formaldehyde', 'Injections', 'Injections, Subcutaneous', 'Kynurenic Acid', 'Male', 'Memantine', 'Motor Activity', 'Nociceptors', 'Phenols', 'Piperazines', 'Piperidines', 'Pruritus', 'Rats', 'Rats, Sprague-Dawley', 'Receptors, N-Methyl-D-Aspartate', 'Synaptic Transmission', 'Time Factors', 'Vibrissae']
| 20,401,359
|
[['B01.050'], ['F01.145.113'], ['A08.186.566.166.686.351'], ['C22.232', 'E05.598.500', 'E05.599.395.080'], ['D27.505.519.625.190.300', 'D27.505.696.577.190.300'], ['C23.888.592.612.330'], ['D02.047.407'], ['E02.319.267.530'], ['E02.319.267.530.620'], ['D03.066.942.505', 'D03.633.100.810.350.400'], ['D02.455.426.100.050.035.500'], ['F01.145.632', 'G11.427.410.698'], ['A08.675.650.915.875', 'A08.800.950.875', 'A11.671.650.915.875'], ['D02.455.426.559.389.657'], ['D03.383.606'], ['D03.383.621'], ['C17.800.685', 'C23.888.885.625'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.750'], ['D12.776.157.530.400.400.500.500', 'D12.776.543.550.450.500.200.500', 'D12.776.543.585.400.500.200.500', 'D12.776.543.750.720.200.450.400.500'], ['G02.111.820.850', 'G04.835.850', 'G07.265.880', 'G11.561.830'], ['G01.910.857'], ['A13.950']]
|
['Organisms [B]', 'Psychiatry and Psychology [F]', 'Anatomy [A]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Exposing women to workplace stress factors as a risk factor for developing arterial hypertension.
|
The purpose of this study is the evaluation of women's exposure to stress-inducing factors at work, definition of a scale of the problem, as well as assessment of the impact of professional work on the value of arterial pressure. The research was conducted on four professional groups of women: working in agriculture, working as clerks, seamstresses, as well as those working as medical representative in the period from August- September 2008 in the Lublin region. A total number of 416 women was examined, ages ranging from 30-40, who had not been previously treated due to arterial hypertension. The women under examination had their arterial blood pressure measured twice on a working day at 08:00 and at 14:00. The values of measurements were averaged. The research tool was also the standardised Questionnaire for Subjective Work Evaluation. The raw result was obtained on the basis of summing up all the points, which were afterwards transformed into 10 standard values. The general result was given in 10 standard values, whereas the results of stress factors were quoted as mean results of raw values and were referred to results defined as high for a given factor. The results obtained were statistically analysed on the basis of t-Student test. The significance level adopted was p<0.05. The results obtained in a particular professional group were compared as well as the impact of socio-demographic variables, such as level of education, marital status, place of residence, on the intensification of stress related to a particular factor of work evaluation, was also analysed. Stress experienced by women at a workplace affects not ony their professional life, but also family life and social intercourses. In the women's opinion, an unpleasant workplace is such a workplace where the feeling of mental workload is connected with the lack of rewards (motivation), uncertainty resulting from organisation of daily chores and lack of support from others. The high general level of stress was noted among the group of women working in agriculture, in pharmaceutical companies, as well as among those who perform physical work (seamstresses). The intensification of stress at a workplace had a considerable impact on the value of arterial pressure among the group of woman medical representatives, as well as among the group of woman office workers. No significant dependencies were concluded between socio-demographic variables and the general level of exposure to intensified stress in the examined professional groups. The above research confirms the need for further examination of the working environment of women and its impact on health. Obviously, attempts should be made in order to improve the conditions of work for women, bearing in mind the fact that the adoption of neutral attitude towards the sexes when assessing risk and undertaking preventive activities may result in the female gender being underestimated or even disregarded.
|
['Adult', 'Female', 'Humans', 'Hypertension', 'Occupational Exposure', 'Poland', 'Risk Factors', 'Stress, Psychological', 'Workplace']
| 21,736,283
|
[['M01.060.116'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C14.907.489'], ['N06.850.460.350.600'], ['Z01.542.248.679'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['F01.145.126.990', 'F02.830.900'], ['N01.824.245.925', 'N04.452.677.975']]
|
['Named Groups [M]', 'Organisms [B]', 'Diseases [C]', 'Health Care [N]', 'Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Psychiatry and Psychology [F]']
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Market fallacies in health economics.
|
Serious methodological errors that plague studies in health economics are examined with the focus on misconceptions about the nature and functions of markets. The belief that market economics do not apply to the medical marketplace involves circular reasoning that treats man-made laws and regulations as though they were unchangeable laws of nature. Arguments against the market provision of health care are questioned and the 'information gap' problem is shown to be aggravated, if not caused, by regulations that prevent normal information flows in the market. Similarly, the contention that health care insurance pushes up costs is criticised on the basis of both theory and empirical evidence. The apparent failure to contain costs may be blamed on legal restrictions, government spending and pressure from medical associations. Confusion between normative theory and positive theory is also examined.
|
['Delivery of Health Care', 'Health Policy', 'Insurance, Health', 'Marketing of Health Services']
| 1,745,941
|
[['N04.590.374', 'N05.300'], ['I01.655.500.608.400', 'I01.880.604.825.608.400', 'N03.623.500.608.428'], ['N03.219.521.576.343'], ['J01.219.687.550', 'N03.219.463.548', 'N05.300.430.500']]
|
['Health Care [N]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Technology, Industry, and Agriculture [J]']
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
| 0
| 1
| 0
|
[Age-related changes in the cholin- and adrenergic innervation of the human heart].
|
The hearts of 35 patients of different age have been studied. The cholinergic innervation reaches full differentiation by the age of 30, its involution begins after 50. The adrenergic innervation is most manifest in children, its involution begins from 30. After 60 the nervous plexuses of the heart are fully devoid of catecholamines. The sensitivity of the myocardium to adrenalin at different age levels is roughly the same.
|
['Adolescent', 'Adult', 'Aging', 'Child', 'Heart', 'Heart Atria', 'Heart Septum', 'Heart Ventricles', 'Humans', 'Middle Aged', 'Receptors, Adrenergic', 'Receptors, Cholinergic']
| 7,311,288
|
[['M01.060.057'], ['M01.060.116'], ['G07.345.124'], ['M01.060.406'], ['A07.541'], ['A07.541.358'], ['A07.541.459'], ['A07.541.560'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['D12.776.543.750.670.300.300', 'D12.776.543.750.695.150.300', 'D12.776.543.750.720.330.300'], ['D12.776.543.750.720.360']]
|
['Named Groups [M]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Organisms [B]', 'Chemicals and Drugs [D]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Enhanced plasma availability of the metabolites of albendazole in fasted adult sheep.
|
The influence of fasting prior to treatment and of dosing rate on the plasma availability and disposition kinetics of albendazole (ABZ) and its sulphoxide (ABZSO) and sulphone (ABZSO2) metabolites was studied in adult sheep grazing on pasture. A micronized suspension of ABZ was administered orally at either 7.5 mg/kg (group A) or 11.3 mg/kg (group C) to sheep fed ad libitum, and at 7.5 mg/kg to sheep subjected to a 24 h fasting period prior to treatment (group B). Blood samples were taken serially over 96 h after treatment, and the plasma was analysed for ABZ and its metabolites by high-performance liquid chromatography. ABZSO and ABZSO2 were recovered from the plasma. Fasting induced marked modifications in the pharmacokinetic behaviour of the ABZ metabolites in sheep. An extended absorption process, with a delayed peak concentration in the plasma, was observed for both metabolites in the fasted sheep. Significantly higher area under the curve (AUC) and peak plasma concentration (Cmax) values were obtained for both metabolites in the fasted animals compared to those fed ad libitum. Delayed elimination with prolonged detection in plasma was also observed in the fasted sheep. Treatment with ABZ at 7.5 mg/kg in the starved animals resulted in bioequivalence to the administration of the compound at a 50% higher dose rate (11.3 mg/kg) in the fed animals. It is suggested that fasting enhances ABZ dissolution and absorption by delaying its passage down the digestive tract.
|
['Albendazole', 'Animals', 'Anthelmintics', 'Area Under Curve', 'Biological Availability', 'Chromatography, High Pressure Liquid', 'Fasting', 'Female', 'Random Allocation', 'Sheep', 'Time Factors']
| 9,090,047
|
[['D02.241.081.251.022', 'D03.633.100.103.070'], ['B01.050'], ['D27.505.954.122.250.075'], ['E05.318.740.200', 'G03.787.101', 'G07.690.725.064', 'N06.850.520.830.200'], ['G03.787.151', 'G07.690.725.129'], ['E05.196.181.400.300'], ['F01.145.407.400', 'G07.203.650.240.587', 'G07.203.650.353.400'], ['E05.318.370.700', 'E05.581.500.805', 'N05.715.360.325.675', 'N06.850.520.445.700'], ['B01.050.150.900.649.313.500.380.791'], ['G01.910.857']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Psychiatry and Psychology [F]']
| 0
| 1
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Protein kinase Cdelta-dependent and -independent signaling in genotoxic response to treatment of desferroxamine, a hypoxia-mimetic agent.
|
Protein kinase C (PKC) plays a critical role in diseases such as cancer, stroke, and cardiac ischemia and participates in a variety of signal transduction pathways including apoptosis, cell proliferation, and tumor suppression. Here, we demonstrate that PKCdelta is proteolytically cleaved and translocated to the nucleus in a time-dependent manner on treatment of desferroxamine (DFO), a hypoxia-mimetic agent. Specific knockdown of the endogenous PKCdelta by RNAi (sh-PKCdelta) or expression of the kinase-dead (Lys376Arg) mutant of PKCdelta (PKCdeltaKD) conferred modulation on the cellular adaptive responses to DFO treatment. Notably, the time-dependent accumulation of DFO-induced phosphorylation of Ser-139-H2AX (gamma-H2AX), a hallmark for DNA damage, was altered by sh-PKCdelta, and sh-PKCdelta completely abrogated the activation of caspase-3 in DFO-treated cells. Expression of Lys376Arg-mutated PKCdelta-enhanced green fluorescent protein (EGFP) appears to abrogate DFO/hypoxia-induced activation of endogenous PKCdelta and caspase-3, suggesting that PKCdeltaKD-EGFP serves a dominant-negative function. Additionally, DFO treatment also led to the activation of Chk1, p53, and Akt, where DFO-induced activation of p53, Chk1, and Akt occurred in both PKCdelta-dependent and -independent manners. In summary, these findings suggest that the activation of a PKCdelta-mediated signaling network is one of the critical contributing factors involved in fine-tuning of the DNA damage response to DFO treatment.
|
['Active Transport, Cell Nucleus', 'Animals', 'Caspase 3', 'Cell Line', 'DNA Damage', 'Deferoxamine', 'Epithelial Cells', 'Hypoxia', 'Oncogene Protein v-akt', 'Protein Kinase C-delta', 'Rats', 'Signal Transduction']
| 17,563,398
|
[['G03.143.310.100', 'G03.143.700.100'], ['B01.050'], ['D08.811.277.656.262.500.126.350.300', 'D08.811.277.656.300.200.126.350.300', 'D12.644.360.075.405.350.300', 'D12.776.476.075.405.350.300'], ['A11.251.210'], ['G05.200'], ['D02.092.570.394.265', 'D02.241.511.372.265'], ['A11.436'], ['C23.888.852.079'], ['D08.811.913.696.620.682.700.586', 'D12.776.624.664.520.750.788'], ['D08.811.913.696.620.682.700.725.400'], ['B01.050.150.900.649.313.992.635.505.700'], ['G02.111.820', 'G04.835']]
|
['Phenomena and Processes [G]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Barrel Jellyfish (Rhizostoma pulmo
|
The jellyfish Rhizostoma pulmo, Macr? 1778 (Cnidaria, Rhizostomae) undergoes recurrent outbreaks in the Mediterranean coastal waters, with large biomass populations representing a nuisance or damage for marine and maritime activities. A preliminary overview of the antioxidant activity (AA) of R. pulmo proteinaceous compounds is provided here based on the extraction and characterization of both soluble and insoluble membrane-fractioned proteins, the latter digested by sequential enzymatic hydrolyses with pepsin and collagenases. All jellyfish proteins showed significant AA, with low molecular weight (MW) proteins correlated with greater antioxidant activity. In particular, collagenase-hydrolysed collagen resulted in peptides with MW lower than 3 kDa, ranging 3⁻10 kDa or 10⁻30 kDa, with AA inversely proportional to MW. No cytotoxic effect was detected on cultured human keratinocytes (HEKa) in a range of protein concentration 0.05⁻20 ìg/mL for all tested protein fractions except for soluble proteins higher than 30 kDa, likely containing the jellyfish venom compounds. Furthermore, hydrolyzed jellyfish collagen peptides showed a significantly higher AA and provided a greater protective effect against oxidative stress in HEKa than the hydrolyzed collagen peptides from vertebrates. Due to a high reproductive potential, jellyfish may represent a potential socioeconomic opportunity as a source of natural bioactive compounds, with far-reaching beneficial implications. Eventually, improvements in processing technology will promote the use of untapped marine biomasses in nutraceutical, cosmeceutical, and pharmaceutical fields, turning marine management problems into a more positive perspective.
|
['Animals', 'Antioxidants', 'Cell Survival', 'Cells, Cultured', 'Collagen', 'Humans', 'Keratinocytes', 'Oxidative Stress', 'Peptides', 'Scyphozoa']
| 30,813,405
|
[['B01.050'], ['D27.505.519.217', 'D27.505.696.706.125', 'D27.720.799.047'], ['G04.346'], ['A11.251'], ['D05.750.078.280', 'D12.776.860.300.250'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A11.409.500', 'A11.436.397'], ['G03.673', 'G07.775.750'], ['D12.644'], ['B01.050.500.308.690']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
The risk of periventricular-intraventricular hemorrhage with vacuum extraction of neonates weighing 2000 grams or less.
|
OBJECTIVE: The objective of this retrospective study was to test the hypothesis that vacuum extraction of neonates with a birth weight < or = 2000 gm is associated with an increased risk of periventricular-intraventricular hemorrhage (PV-IVH).STUDY DESIGN: One hundred sixty-eight neonates weighing < or = 2000 gm were entered into this case control study. Fifty-six neonates delivered by silicone-rubber vacuum comprised the study group. For every infant born by vacuum-assisted delivery, two spontaneously delivered neonates served as controls (n = 112) and were matched for best obstetric gestational age (within 1 week), delivery date within 1 year, and birth weight < or = 2000 gm. Maternal and neonatal medical records were reviewed for demographic variables, antenatal complications, indication for vacuum-assisted delivery, neonatal birth weight, Apgar scores, umbilical cord blood gas values, and neonatal morbidity including the incidence of PV-IVH. All perinatal outcome variables were compared between the vacuum-assisted and spontaneously delivered groups.RESULTS: Both groups were similar with regard to maternal age, parity, antepartum complications, gestational age at delivery, neonatal birth weight, and gender. Both groups were also similar with regard to the incidence of 1-minute Apgar score < 5, small for gestational age, cephalhematoma, birth trauma, respiratory distress syndrome, hyperbilirubinemia, and sepsis. There was a significant increased incidence of 5-minute Apgar scores < 7 in the vacuum-assisted group (p = 0.04). No significant difference was observed in the incidence of PV-IVH grades 1 through 4 (21.4% vacuum group vs 16.1% control group, odds ratio 1.42, 0.58 to 3.45).CONCLUSION: Indicated use of the silicone-rubber vacuum to assist vaginal delivery of neonates weighing < or = 2000 gm does not appear to be associated with an increased risk of PV-IVH or other neonatal complications.
|
['Case-Control Studies', 'Cerebral Hemorrhage', 'Confidence Intervals', 'Female', 'Humans', 'Incidence', 'Infant, Low Birth Weight', 'Infant, Newborn', 'Leukomalacia, Periventricular', 'Odds Ratio', 'Pregnancy', 'Pregnancy Outcome', 'Retrospective Studies', 'Risk Factors', 'Survival Rate', 'Vacuum Extraction, Obstetrical']
| 9,069,063
|
[['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['C10.228.140.300.535.200', 'C14.907.253.573.200', 'C23.550.414.913.100'], ['E05.318.740.275', 'N05.715.360.750.220', 'N06.850.520.830.275'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.308.985.525.375', 'N01.224.935.597.500', 'N06.850.505.400.975.525.375', 'N06.850.520.308.985.525.375'], ['M01.060.703.520.460'], ['M01.060.703.520'], ['C10.228.140.300.700', 'C10.228.140.461.550', 'C14.907.253.612', 'C16.614.521.450'], ['E05.318.740.600.600', 'G17.680.500', 'N05.715.360.750.625.590', 'N06.850.520.830.600.600'], ['G08.686.784.769'], ['E01.789.700', 'G08.686.784.769.496'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['E05.318.308.985.550.900', 'N01.224.935.698.826', 'N06.850.505.400.975.550.900', 'N06.850.520.308.985.550.900'], ['E04.520.252.875.970']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Diseases [C]', 'Organisms [B]', 'Named Groups [M]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Identification of proteins differentially expressed in human monocytes exposed to Porphyromonas gingivalis and its purified components by high-throughput immunoblotting.
|
To characterize the roles of Porphyromonas gingivalis and its components in disease processes, we investigated the cytokine profiles induced by live P. gingivalis, its lipopolysaccharide (LPS), and its major fimbrial protein, fimbrillin (FimA). A cytokine antibody array revealed that human monocyte-derived macrophages were induced to produce chemokines (e.g., monocyte chemoattractant protein 1, macrophage inflammatory protein 1beta [MIP-1beta], and MIP-3alpha) as early as 1 h after exposure to P. gingivalis, with production declining after 24 h of exposure. As expected, an extensive repertoire of inflammatory mediators increased subsequent to infection, most predominantly tumor necrosis factor alpha (TNF-alpha), interleukin 1beta (IL-1beta), IL-6, IL-10, and granulocyte-macrophage colony-stimulating factor. The induction of cytokines by P. gingivalis was not triggered simply by bacterial cell surface components, since purified P. gingivalis LPS and FimA induced similar patterns of cytokines, while the pattern of cytokines induced by live P. gingivalis was significantly different, indicating that the host defense system senses live bacteria differently than it does the cell surface components LPS and FimA. To further understand the mechanisms by which live P. gingivalis and its components exert their effects, we used a high-throughput immunoblot screening approach (Becton-Dickinson PowerBlot) to analyze intracellular proteins involved in P. gingivalis infection in human macrophages. Exposure of human macrophages to either live P. gingivalis, its LPS, or its FimA protein led to the up-regulation of 12, 8, and 10 proteins and the down-regulation of 15, 8, and 17 proteins, respectively. The expression of proteins involved in gene transcription (e.g., monocyte enhancer factor 2D [MEF2D], signal transducer and activator of transcription 1 [STAT1], STAT3, STAT6, and IL enhancer binding factors [ILF3]), of protein kinases (e.g., mitogen-activated protein kinase 3 [MAPK3], MAP3K8, double-stranded RNA-activated protein kinase [PRKR], and MAP2K4), and of proteins involved in immune responses (e.g., TNF super family member 6 [TNFSF6] and interferon-induced protein with tetratricopeptide repeat 4 [IFIT4]), apoptosis (e.g., genes associated with retinoid interferon-induced mortality 19 [GRIM19]), and other fundamental cellular processes (e.g., clathrin heavy-chain polypeptide, culreticulin, and Ras-associated protein RAB27A) was found to be modulated differentially by P. gingivalis, LPS, and FimA. These differential changes are interpreted as preferential signal pathway activation in host immune/inflammatory responses to P. gingivalis infection.
|
['Apoptosis', 'Cells, Cultured', 'Cytokines', 'Fimbriae Proteins', 'Gene Expression Regulation', 'Humans', 'Immunity, Innate', 'Immunoblotting', 'Lipopolysaccharides', 'Macrophages', 'Monocytes', 'Porphyromonas gingivalis']
| 16,428,770
|
[['G04.146.954.035'], ['A11.251'], ['D12.644.276.374', 'D12.776.467.374', 'D23.529.374'], ['D12.776.097.120.425', 'D12.776.543.100.300'], ['G05.308'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G12.450.564'], ['E05.478.566.320', 'E05.601.470.320'], ['D09.400.500', 'D09.698.718.450', 'D10.494', 'D23.050.161.616.525', 'D23.946.123.329.500'], ['A11.329.372', 'A11.627.482', 'A11.733.397', 'A15.382.670.522', 'A15.382.680.397'], ['A11.118.637.555.652', 'A11.148.580', 'A11.627.624', 'A11.733.547', 'A15.145.229.637.555.652', 'A15.378.316.580', 'A15.382.490.555.652', 'A15.382.670.547', 'A15.382.680.547'], ['B03.440.080.094.625.515', 'B03.440.425.410.194.625.515']]
|
['Phenomena and Processes [G]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
On mechanisms of sex differences in chemical carcinogenesis: effects of implantation of ectopic pituitary grafts on the early stages of liver carcinogenesis in the rat.
|
The resistant hepatocyte model was used to investigate the influence of pituitary factors on the early events of chemical carcinogenesis in rat liver. Diethylnitrosamine (DEN), 200 mg/kg body weight, was used as an initiator of enzyme altered foci. Two weeks after initiation the rats were placed on a 0.02% (w/w) 2-acetylaminofluorene (2-AAF) diet for two weeks. Partial hepatectomy (70%) was performed three weeks after initiation. The rats were killed four to six weeks after DEN initiation. Sex differences in area/foci as well as in area ratio (mm2 foci/cm2 liver section) were found in liver sections from sexually mature male and female rats (male greater than female) of both the Sprague-Dawley and Wistar strains. Ectopic pituitary grafts (PG:s) implanted under the kidney capsule of male Wistar rats one week before DEN initiation and removed by unilateral nephrectomy one week after initiation did not influence the number or area of enzyme altered foci as compared with sham operated male rats. On the other hand, PG:s implanted one week before 2-AAF selection in male Wistar rats and allowed to remain until the rats were killed two weeks after the 2-AAF selection period, decreased the area ratio to a level close to that of sham operated female rats, whereas no effect on the number of enzyme altered foci was found. The results suggest that the hypothalamo-pituitary axis may be involved in the regulation of early stages of liver carcinogenesis.
|
['2-Acetylaminofluorene', 'Animals', 'Diethylnitrosamine', 'Female', 'Hepatectomy', 'Hypothalamo-Hypophyseal System', 'Liver Neoplasms, Experimental', 'Male', 'Pituitary Gland', 'Rats', 'Rats, Inbred Strains', 'Sex Factors', 'Steroids', 'Testosterone']
| 6,488,447
|
[['D02.065.064.150', 'D02.241.081.018.110.080', 'D02.455.426.559.847.389.050', 'D04.615.389.050'], ['B01.050'], ['D02.654.442.200'], ['E04.210.556'], ['A06.688.357', 'A08.186.211.180.497.352.435', 'A08.186.211.200.317.357.352.435', 'A08.713.357'], ['C04.588.274.623.460', 'C04.619.540', 'C06.301.623.460', 'C06.552.697.580', 'E05.598.500.496.750'], ['A06.300.747', 'A06.688.357.750', 'A08.186.211.180.497.352.435.500', 'A08.186.211.200.317.357.352.435.500', 'A08.713.357.750'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.050.199.520.760', 'B01.050.150.900.649.313.992.635.505.700.400'], ['N05.715.350.675', 'N06.850.490.875'], ['D04.210.500'], ['D04.210.500.054.079.429.824', 'D06.472.334.851.968.984']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Diseases [C]', 'Health Care [N]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
[Rectal cancer (a differential electron microscopic diagnosis)].
|
A tumor of the rectum in a woman of 74 is described. The analysis of the tumor in light microscope suggested a low-differentiated cancer or melanoma. Electron microscopic examination of the tumor metastasis into a lymph node showed most of the tumor cells to have low differentiation and contain no ultrastructural organspecific markers. However, in the tumor cells comprising a small group, typical tonofibrils and desmosomes were found which substantiated the diagnosis of squamous cell carcinoma.
|
['Aged', 'Biopsy', 'Carcinoma, Squamous Cell', 'Diagnosis, Differential', 'Female', 'Humans', 'Lymphatic Metastasis', 'Microscopy, Electron', 'Rectal Neoplasms']
| 7,125,942
|
[['M01.060.116.100'], ['E01.370.225.500.384.100', 'E01.370.225.998.054', 'E01.370.388.100', 'E04.074', 'E05.200.500.384.100', 'E05.200.998.054', 'E05.242.384.100'], ['C04.557.470.200.400', 'C04.557.470.700.400'], ['E01.171'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C04.697.650.560', 'C23.550.727.650.560'], ['E01.370.350.515.402', 'E05.595.402'], ['C04.588.274.476.411.307.790', 'C06.301.371.411.307.790', 'C06.405.249.411.307.790', 'C06.405.469.491.307.790', 'C06.405.469.860.180.500']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Risk factors in cesarean section infection.
|
Factors associated with risk of postoperative infection after cesarean section were studied in 321 patients not given antibiotic prophylaxis. Infections occurred in 56 (25%) of the 228 patients who were delivered by emergency cesarean section and in eight (9%) of the 93 patients who underwent elective surgery (P less than .01). These frequencies corresponded well with the infection rates reported after administration of antibiotics in other studies. Risk factors were: duration of operation more than one hour; blood loss more than 800 ml; presence of Staphylococcus aureus in the nares; signs of intrauterine infection before surgery; and failure of progress in labor. The results indicated that obstetric interventions had been performed more frequently in patients at risk of infection, rather than being the real cause of the infections. The importance of strict preoperative hygienic routine is discussed.
|
['Bacterial Infections', 'Cesarean Section', 'Endometritis', 'Female', 'Hemorrhage', 'Humans', 'Postoperative Complications', 'Pregnancy', 'Pregnancy, Prolonged', 'Risk', 'Staphylococcal Infections', 'Staphylococcus aureus', 'Streptococcal Infections', 'Surgical Wound Infection', 'Time Factors', 'Uterine Diseases']
| 6,866,354
|
[['C01.150.252'], ['E04.520.252.500'], ['C13.351.500.056.750.249', 'C13.351.500.852.299'], ['C23.550.414'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C23.550.767'], ['G08.686.784.769'], ['C13.703.805'], ['E05.318.740.600.800', 'G17.680.750', 'N05.715.360.750.625.700', 'N06.850.520.830.600.800'], ['C01.150.252.410.868'], ['B03.300.390.400.800.750.100', 'B03.353.500.750.750.100', 'B03.510.100.750.750.100', 'B03.510.400.790.750.100'], ['C01.150.252.410.890'], ['C01.947.692', 'C23.550.767.925'], ['G01.910.857'], ['C13.351.500.852']]
|
['Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Health Care [N]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Effects of biomineralization peptide topology on the structure and catalytic activity of Pd nanomaterials.
|
Highly branched, coral-like Pd nanostructures were formed using a biomineralization peptide conjugated to an oligomeric peptide that simultaneously controls the spatial orientation, arrangement and valency. The Pd nanocoral showed very high catalytic activity in the reduction of nitrophenol. The results highlight the importance of topological arrangement in nanostructure formation and catalytic activity.
|
['Amino Acid Sequence', 'Catalysis', 'Microscopy, Electron, Scanning Transmission', 'Minerals', 'Molecular Sequence Data', 'Molecular Structure', 'Nanostructures', 'Palladium', 'Peptides']
| 24,963,622
|
[['G02.111.570.060', 'L01.453.245.667.060'], ['G02.130'], ['E01.370.350.515.402.580.480', 'E05.595.402.580.480'], ['D01.578'], ['L01.453.245.667'], ['G02.111.570', 'G02.466'], ['J01.637.512'], ['D01.268.556.680', 'D01.268.956.718', 'D01.552.544.680'], ['D12.644']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Technology, Industry, and Agriculture [J]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 0
|
Evaluation of a personal-computer-based teleradiology system serving an isolated Canadian community.
|
OBJECTIVE: To evaluate a personal-computer-based teleradiology system for the interpretation of radiologic studies from an isolated community.METHODS: During a 5-month study period, 240 radiologic studies from the Grand Manan Hospital, Grand Manan, NB, were digitized and transmitted via telephone lines to the Saint John Regional Hospital, NB, for interpretation. The first 110 cases were interpreted with the use of a 1 K x 1 K monitor, and the remaining cases were interpreted with a 2 K x 2 K monitor. The teleradiology image reports were compared with the plain film reports for each case and discrepancies were identified. A panel reviewed all clinically significant discrepant cases to determine the source of the discrepancy.RESULTS: There was 90.9% concordance between the teleradiology and plain film reports in the studies interpreted with the 1 K x 1 K monitor and a 88.4% concordance in the studies interpreted with the 2 K x 2 K monitor. Only 1.7% (4/240) of the discrepancies were attributed to an inadequate teleradiology digital image.CONCLUSION: A personal-computer-based teleradiology system can be used to provide diagnostic imaging service of high quality to an isolated community.
|
['Computer Communication Networks', 'Computer Terminals', 'Evaluation Studies as Topic', 'Humans', 'Image Processing, Computer-Assisted', 'Microcomputers', 'New Brunswick', 'Observer Variation', 'Pilot Projects', 'Radiography', 'Remote Consultation', 'Rural Health Services', 'Teleradiology']
| 9,494,458
|
[['L01.224.230.110'], ['L01.224.230.260.115.500'], ['E05.337', 'N05.715.360.335'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['L01.224.308'], ['L01.224.230.260.550'], ['Z01.107.567.176.494'], ['E01.354.753', 'N02.421.450.600', 'N05.715.350.150.675', 'N06.850.490.500.250'], ['E05.318.372.750', 'E05.337.737', 'N05.715.360.330.720', 'N06.850.520.450.720'], ['E01.370.350.700'], ['L01.178.847.652.550', 'N04.452.758.849.550', 'N04.590.374.800.550'], ['N02.421.816'], ['E05.920.700', 'H02.010.850.700', 'H02.403.840.700', 'L01.178.847.652.700', 'N04.452.515.825.500', 'N04.590.374.800.700']]
|
['Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Geographicals [Z]', 'Disciplines and Occupations [H]']
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 1
|
Coffee polyphenols protect human plasma from postprandial carbonyl modifications.
|
The antioxidant capability of coffee polyphenols to inhibit red-meat lipid peroxidation in stomach medium and absorption into blood of malondialdehyde (MDA) in humans was studied. Roasted-ground coffee polyphenols that were found to inhibit lipid peroxidation in stomach medium are 2- to 5-fold more efficient antioxidant than those found in instant coffee. Human plasma from ten volunteers analyzed after a meal of red-meat cutlets (250 g) revealed a rapid accumulation of MDA. The accumulation of MDA in human plasma modified low-density lipoprotein is known to trigger atherogenesis. Consumption of 200 mL roasted coffee by ten volunteers during a meal of red-meat cutlets, resulted after 2 and 4 h in the inhibition by 80 and 50%, respectively, of postprandial plasma MDA absorption. The results obtained in vitro simulated stomach model on MDA accumulation were predictive for the amount of MDA absorbed into circulating human plasma, in vivo. Timing the consumption of coffee during the meals may make it a very active functional food.
|
['Animals', 'Antioxidants', 'Cattle', 'Coffee', 'Gastric Mucosa', 'Humans', 'Lipid Peroxidation', 'Lipoproteins, LDL', 'Malondialdehyde', 'Meat', 'Polyphenols', 'Postprandial Period', 'Stomach']
| 23,322,503
|
[['B01.050'], ['D27.505.519.217', 'D27.505.696.706.125', 'D27.720.799.047'], ['B01.050.150.900.649.313.500.380.271'], ['D20.215.784.249', 'G07.203.100.325', 'J02.200.325'], ['A03.556.875.875.440', 'A10.615.550.291'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G02.111.515', 'G03.295.531.587'], ['D10.532.515', 'D12.776.521.550'], ['D02.047.700'], ['G07.203.300.600', 'J02.500.600'], ['D02.455.426.559.389.657.715', 'D03.633.100.150.266.450.260.777'], ['G10.261.700'], ['A03.556.875.875']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Technology, Industry, and Agriculture [J]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
The effect of intracranial stent implantation on the curvature of the cerebrovasculature.
|
BACKGROUND AND PURPOSE: Recently, the use of stents to assist in the coiling and repair of wide-neck aneurysms has been shown to be highly effective; however, the effect of these stents on the RC of the parent vessel has not been quantified. The purpose of this study was to quantify the effect of intracranial stenting on the RC of the implanted artery using 3D datasets.MATERIALS AND METHODS: Twenty-four patients receiving FDA-approved neurovascular stents to support coil embolization of brain aneurysms were chosen for this study. The stents were located in the ICA, ACA, or MCA. We analyzed C-arm rotational angiography and contrast-enhanced cone beam CT datasets before and after stent implantation, respectively, to ascertain changes in vessel curvature. The images were reconstructed, and the vessel centerline was extracted. From the centerline, the RC was calculated.RESULTS: The average implanted stent length was 25.4 ± 5.8 mm, with a pre-implantation RC of 7.1 ± 2.1 mm and a postimplantation RC of 10.7 ± 3.5 mm. This resulted in a 3.6 ± 2.7 mm change in the RC due to implantation (P < .0001), more than a 50% increase from the pre-implantation value. There was no difference in the change of RC for the different locations studied. The change in RC was not impacted by the extent of coil packing within the aneurysm.CONCLUSIONS: The implantation of neurovascular stents can be shown to have a large impact on the RC of the vessel. This will lead to a change in the local hemodynamics and flow pattern within the aneurysm.
|
['Adolescent', 'Adult', 'Aged', 'Blood Vessel Prosthesis', 'Female', 'Humans', 'Intracranial Aneurysm', 'Male', 'Mechanical Thrombolysis', 'Middle Aged', 'Prosthesis Implantation', 'Radiography', 'Stents', 'Treatment Outcome', 'Young Adult']
| 22,538,075
|
[['M01.060.057'], ['M01.060.116'], ['M01.060.116.100'], ['E07.695.110'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C10.228.140.300.510.600', 'C14.907.055.635', 'C14.907.253.560.300'], ['E02.631', 'E04.100.814.842.500'], ['M01.060.116.630'], ['E04.650'], ['E01.370.350.700'], ['E07.695.750'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Diseases [C]', 'Health Care [N]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Mouse fetal trisomy 13 and hypotrophy of the spinal cord: effect on calbindin-D28k and calretinin expressed by neurons of the spinal cord and dorsal root ganglia.
|
Trisomy 13 was detected in 10% of mouse embryos obtained from pregnant females which were doubly heterozygous for Robertsonian chromosomes involving chromosome 13. The developing dorsal root ganglia and spinal cords were examined in trisomy 13 and littermate control mice between days 12 and 18 of gestation (E12-18). The overall size of the dorsal root ganglia and number of ganglion cells within a given ganglion were not altered, but the number of neurons immunoreactive for calbindin and calretinin was reduced. The trisomic spinal cord was reduced in size with neurons lying in a tightly compact distribution in the gray matter. In trisomic fetuses, the extent of the neuropil of the spinal cord was reduced, and may represent a diminished field of interneuronal connectivity, due to reduced arborization of dendritic processes of the neurons present, particularly of calbindin-immunostained neurons. Furthermore, the subpopulation of calretinin-immunoreactive neurons and axons was also reduced in developing trisomic gray and white matter, respectively. Thus, overexpression of genes on mouse chromosome 13 exerts a deleterious effect on the development of neuropil, affecting both dendritic and axonal arborization in the trisomy 13 mouse. The defect of calbindin or calretinin expression by subsets of dorsal root ganglion or spinal cord neurons may result from deficient cell-to-cell interactions with targets which are hypoplastic.
|
['Animals', 'Calbindin 1', 'Calbindin 2', 'Calbindins', 'Embryo, Mammalian', 'Embryonic and Fetal Development', 'Female', 'Ganglia, Spinal', 'Humans', 'Immunohistochemistry', 'Mice', 'Molecular Weight', 'Nerve Tissue Proteins', 'Neurons', 'Neurons, Afferent', 'S100 Calcium Binding Protein G', 'Spinal Cord', 'Trisomy']
| 8,309,546
|
[['B01.050'], ['D12.776.157.125.090.124', 'D12.776.631.087'], ['D12.776.157.125.090.249'], ['D12.776.157.125.090'], ['A16.254'], ['G07.345.500.325', 'G08.686.784.170'], ['A08.340.390.340', 'A08.800.350.340', 'A08.800.800.720.725.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.225.500.607.512', 'E01.370.225.750.551.512', 'E05.200.500.607.512', 'E05.200.750.551.512', 'E05.478.583', 'H01.158.100.656.234.512', 'H01.158.201.344.512', 'H01.158.201.486.512', 'H01.181.122.573.512', 'H01.181.122.605.512'], ['B01.050.150.900.649.313.992.635.505.500'], ['G02.494'], ['D12.776.631'], ['A08.675', 'A11.671'], ['A08.675.650', 'A11.671.650'], ['D12.776.157.125.090.500', 'D12.776.157.125.750.750'], ['A08.186.854'], ['C23.550.210.050.750', 'C23.550.210.182.500', 'G05.365.590.175.050.750', 'G05.365.590.175.183.500', 'G05.700.131.750']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
Mentally simulated movements in virtual reality: does Fitts's law hold in motor imagery?
|
This study was designed to investigate mentally simulated actions in a virtual reality environment. Naive human subjects (n = 15) were instructed to imagine themselves walking in a three-dimensional virtual environment toward gates of different apparent widths placed at three different apparent distances. Each subject performed nine blocks of six trials in a randomised order. The response time (reaction time and mental walking time) was measured as the duration between an acoustic go signal and a motor signal produced by the subject. There was a combined effect on response time of both gate width and distance. Response time increased for decreasing apparent gate widths when the gate was placed at different distances. These results support the notion that mentally simulated actions are governed by central motor rules.
|
['Adult', 'Computer Graphics', 'Female', 'Humans', 'Imagination', 'Male', 'Movement', 'Time Perception', 'Walking']
| 8,788,865
|
[['M01.060.116'], ['L01.224.108', 'L01.296.110'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F02.463.188.634'], ['G07.568', 'G11.427.410'], ['F02.463.593.857'], ['G11.427.410.568.900', 'G11.427.410.698.277.937', 'I03.350.937', 'I03.450.642.845.940']]
|
['Named Groups [M]', 'Information Science [L]', 'Organisms [B]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]', 'Anthropology, Education, Sociology, and Social Phenomena [I]']
| 0
| 1
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 1
| 0
| 0
|
Are insulin autoantibodies markers for insulin-dependent diabetes mellitus?
|
Recent studies have shown that insulin autoantibodies occur in patients with newly diagnosed insulin-dependent diabetes mellitus (IDDM) before exogenous insulin treatment. Our study was designed to test the hypothesis that insulin autoantibodies, like cytoplasmic islet cell antibodies (ICAs), can identify individuals with ongoing autoimmune beta-cell destruction and increased risk of IDDM development. Insulin autoantibodies detected by use of a radioligand-binding assay were found in 1.4% of normal controls, 4% of first-degree relatives of IDDM patients, and in 37% of newly diagnosed IDDM patients. A strong positive correlation between insulin autoantibodies and ICAs was observed. HLA typing of insulin-autoantibody-positive first-degree relatives of IDDM patients, as well as in the general population, revealed a strong association with HLA-DR3 and/or-DR4, suggesting that insulin autoantibodies are restricted to persons genetically susceptible to IDDM. In an ongoing study of beta-cell function in ICA-positive nondiabetic individuals, the additional presence of insulin autoantibodies significantly increased the likelihood of beta-cell dysfunction. After intravenous glucose stimulation, insulinopenia was present in 70% of ICA and insulin-autoantibody-positive individuals in contrast to only 23% of ICA-positive, insulin-autoantibody-negative persons. These data document a significant association between insulin autoantibodies and ICAs and support the contention that insulin autoantibodies, like ICAs, are markers of ongoing beta-cell destruction.
|
['Autoantibodies', 'Child', 'Diabetes Mellitus, Type 1', 'Family', 'Female', 'HLA Antigens', 'HLA-DR Antigens', 'Histocompatibility Antigens Class II', 'Humans', 'Insulin', 'Male']
| 3,525,287
|
[['D12.776.124.486.485.114.323', 'D12.776.124.790.651.114.323', 'D12.776.377.715.548.114.323'], ['M01.060.406'], ['C18.452.394.750.124', 'C19.246.267', 'C20.111.327'], ['F01.829.263', 'I01.880.853.150'], ['D23.050.301.500.450', 'D23.050.705.552.450'], ['D12.776.395.550.509.400.440', 'D12.776.543.550.440.400.440', 'D23.050.301.500.400.400.440', 'D23.050.301.500.450.400.440', 'D23.050.705.552.410.400.440', 'D23.050.705.552.450.400.440'], ['D12.776.395.550.509', 'D12.776.543.550.440', 'D23.050.301.500.400', 'D23.050.705.552.410'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D06.472.699.587.200.500.625', 'D12.644.548.586.200.500.625']]
|
['Chemicals and Drugs [D]', 'Named Groups [M]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
|
Toxicological responses of Cyprinus carpio exposed to a commercial formulation containing glyphosate.
|
The effects of commercial glyphosate herbicide formulation on the activity of acetylcholinesterase (AChE) enzyme and oxidative stress were studied in Cyprinus carpio exposed for 96 h to 0.0, 0.5, 2.5, 5.0 and 10.0 mg/L and then allowed to equal recovery period in water without herbicide. The activity of AChE was inhibited in the brain and in the muscle after exposure. However, after recovery period brain and muscle AChE activity increased. Brain thiobarbituric acid reactive species (TBARS) were measured as an indicator of oxidative stress. Increased TBARS levels were observed with all concentrations tested of the glyphosate formulation, and remained increased after the recovery period. The results recorded clearly indicate lipid peroxidation and anti-AChE action induced by Roundup(®) exposure.
|
['Acetylcholinesterase', 'Animals', 'Brain', 'Carps', 'Dose-Response Relationship, Drug', 'Environmental Monitoring', 'Glycine', 'Herbicides', 'Lipid Peroxidation', 'Muscles', 'Oxidative Stress', 'Thiobarbituric Acid Reactive Substances', 'Water Pollutants, Chemical']
| 21,931,962
|
[['D08.811.277.352.100.170.176'], ['B01.050'], ['A08.186.211'], ['B01.050.150.900.493.200.244.248'], ['G07.690.773.875', 'G07.690.936.500'], ['N06.850.460.350.080', 'N06.850.780.375'], ['D12.125.481'], ['D27.720.031.700.366', 'D27.888.723.366'], ['G02.111.515', 'G03.295.531.587'], ['A02.633', 'A10.690'], ['G03.673', 'G07.775.750'], ['D02.047.700.700', 'D27.720.470.410.750'], ['D27.888.284.903.655']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Health Care [N]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
The dynamics of reactive oxygen species in photodynamic therapy with tetra sulfophenyl-porphyrin.
|
Photodynamic therapy (PDT) is a promising therapy especially in skin cancer, using the systemic administration of a photosensitizer (PS), followed by the local irradiation of the tumor with visible light. The antitumor effects of PDT are determined especially by the generation of cytotoxic reactive oxygen species (ROS). The 5,10,15,20-tetra-sulfo-phenyl-porphyrin (TSPP) is a synthetic photosensitizer, which proved its efficiency in in vitro studies. Our study evaluates the effects of PDT with TSPP upon the tumor levels of ROS and upon the metalloproteinases 2 (MMP2) activities on Wistar male rats bearing 256 Walker carcinosarcoma in correlation with the accumulation of PS in the tumor and with the intratumor histological alterations. The evaluations were performed dynamically, at 3 hours, 6 hours, 24 hours and 14 days after the PDT with TSPP. Our results emphasize that 24 hours after the PDT with TSPP, the ROS generation increases, as revealed by protein carbonyls and malondialdehyde levels and the antioxidant capacity (hydrogen donors, thiol groups) decreases in the tumor tissue. These parameters were correlated with the appearance of the histological disorders. The MMP-2 activity increases exponentially in the 24 hours-14 days post PDT interval. PDT with TSPP offers, in vivo , consistent results regarding ROS generation, MMP2 activation and cytotoxic capacity.
|
['Animals', 'Antioxidants', 'Carcinoma 256, Walker', 'Kinetics', 'Male', 'Matrix Metalloproteinase 2', 'Oxidative Stress', 'Photochemotherapy', 'Photosensitizing Agents', 'Porphyrins', 'Rats', 'Rats, Wistar', 'Reactive Oxygen Species', 'Time Factors']
| 20,233,689
|
[['B01.050'], ['D27.505.519.217', 'D27.505.696.706.125', 'D27.720.799.047'], ['C04.557.435.290.210', 'C04.557.450.795.290.210', 'C04.619.045'], ['G01.374.661', 'G02.111.490'], ['D08.811.277.656.300.480.205.352', 'D08.811.277.656.300.480.252.420', 'D08.811.277.656.300.480.525.700.150', 'D08.811.277.656.675.374.205.352', 'D08.811.277.656.675.374.252.420', 'D08.811.277.656.675.374.525.700.150', 'D12.644.276.848.150', 'D12.776.467.836.150'], ['G03.673', 'G07.775.750'], ['E02.186.500', 'E02.319.685', 'E02.774.722'], ['D27.505.954.444.600', 'D27.505.954.600.710'], ['D03.383.129.578.840.500', 'D03.633.400.909.500', 'D04.345.783.500', 'D23.767.727'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.900'], ['D01.339.431', 'D01.650.775'], ['G01.910.857']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Simultaneous endoscopic full-thickness resection of two synchronous colonic granular cell tumours.
|
Granular cell tumours (GCTs) are rare soft tissue tumours originating from Schwann cells. Due to potential malignant transformation, complete endoscopic resection should be aimed for. We report on a 49-year-old patient with two synchronous GCTs found in the caecum and the ascending colon, respectively. Synchronous endoscopic full-thickness resection (EFTR) using an all-in-one full-thickness resection device (FTRD) was performed under propofol sedation. Completeness of resection was proven histologically. No adverse events occurred. We report safe and complete simultaneous EFTR of two synchronous colonic GCTs.
|
['Cecum', 'Colon, Ascending', 'Colonic Neoplasms', 'Early Detection of Cancer', 'Endoscopic Mucosal Resection', 'Granular Cell Tumor', 'Humans', 'Male', 'Middle Aged', 'Treatment Outcome']
| 29,391,355
|
[['A03.556.124.526.209', 'A03.556.249.249.209'], ['A03.556.124.526.356.333', 'A03.556.249.249.356.333'], ['C04.588.274.476.411.307.180', 'C06.301.371.411.307.180', 'C06.405.249.411.307.180', 'C06.405.469.158.356.180', 'C06.405.469.491.307.180'], ['E01.390.500'], ['E01.370.372.250.250.250', 'E01.370.388.250.250.250.230', 'E04.210.240.250.230', 'E04.502.250.250.250.230'], ['C04.557.450.590.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Anatomy [A]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Named Groups [M]', 'Health Care [N]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
The effectiveness and side effects of 0.1% and 0.2% chlorhexidine mouthrinses: a clinical study.
|
OBJECTIVE: The study compared two commercial chlorhexidine mouthrinses (Chlorhexamed 0.1% and Corsodyl 0.2%) for their effects on dental plaque and gingival inflammation, their side effects (eg, tooth staining and mucosal irritation), and patient acceptance.METHOD AND MATERIALS: One hundred thirty healthy volunteers were randomly distributed into two groups of 65 each. Each volunteer had gingivitis or chronic marginal periodontitis and used the rinse two times a day for 4 weeks. The sulcular bleeding index, approximal plaque index, gingival index, and a discoloration index were taken at baseline and once a week thereafter. The patients were questioned about taste disturbances, mucosal irritation, and their perception of the taste of the mouthrinse.RESULTS: In both groups, after 4 weeks, the mean sulcular bleeding index, approximal plaque index, and gingival index scores had decreased significantly. The discoloration index had increased significantly in both groups. There were no statistically significant differences between the two mouthrinses in any of these measurements. There were no significant differences in side effects reported by the two groups.CONCLUSION: The increase in concentration of chlorhexidine provided no clinical advantages or disadvantages.
|
['Adult', 'Chlorhexidine', 'Dental Plaque', 'Dental Plaque Index', 'Dose-Response Relationship, Drug', 'Gingivitis', 'Humans', 'Male', 'Mouthwashes', 'Periodontal Index', 'Periodontitis', 'Taste Disorders', 'Tooth Discoloration']
| 9,759,061
|
[['M01.060.116'], ['D02.078.370.141.100'], ['C07.793.208.377'], ['E05.318.308.980.438.300.300', 'E06.208.250', 'N05.715.360.300.800.438.300.325', 'N06.850.520.308.980.438.300.300', 'N06.890.160.090'], ['G07.690.773.875', 'G07.690.936.500'], ['C01.408', 'C07.465.714.258.480'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D25.583', 'D27.720.102.583', 'D27.720.269.583', 'J01.637.051.583'], ['E05.318.308.980.438.300.725', 'E06.208.720', 'E06.721.658', 'N05.715.360.300.800.438.300.690', 'N06.850.520.308.980.438.300.725', 'N06.890.160.215'], ['C07.465.714.533'], ['C10.597.751.861', 'C23.888.592.763.861'], ['C07.793.735']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Technology, Industry, and Agriculture [J]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 1
| 0
|
Haploidentical stem cell transplantation for acute leukemia.
|
PREMISE: Since March 1993, 133 patients with high-risk acute leukemia (66 AML, 67 ALL) have received a megadose of T-cell depleted hematopoietic stem cells. The 1993-95 conditioning protocol included TBI, thiotepa, ATG and CY for 36 patients who received an inoculum made up of lectin-separated bone marrow and PBPCs. After 1995, to minimise the extra-hematological toxicity of the conditioning and eliminate GvHD, we substituted fludarabine for CY in the conditioning and PBPCs were depleted of T-cells by a positive selection of the CD34+ cells using CellPro (n=44 patients) or, since January 1999, CliniMacs (n = 53 patients). A later modification to the protocol in January 1999 was the suspension of post transplant G-CSF. WORK IN PROGRESS: We report here the results in the last 53 acute leukemia patients all of whom were transplanted under our modified protocol. Ages ranged from 9 to 62 years with a median of 38 years for the 33 patients with AML and 23 for the 20 with ALL. All were at high risk because 25 were actually in relapse at transplant, 16 were in second or later CR and even the 12 patients in CR1 were at high risk because of the unfavourable prognostic features. Overall 52/53 patients (98%) engrafted. The TBI-Fludarabine-based conditioning was well tolerated even in the 14 patients between 45 and 62 years of age. There was no veno-occlusive disease of the liver and the incidence of severe mucositis was low. Even though no post-transplant immunosuppressive therapy was given, acute GvHD grade > or = II occurred in only 4 cases and only one progressed to chronic GvHD. Overall, 16 patients (30%) have died of non-leukemic causes. Relapses occurred mainly in patients who were already in relapse at transplant (12/25). Only 3 of the 28 who were in any CR at transplant have so far relapsed. As our group has already shown, donor-vs-recipient NK cell alloreactivity exerts a specific graft-vs-AML effect in the absence of GvHD. In fact, leukemia relapse was largely controlled in AML recipients whose donor was NK alloreactive, with only 2 out of 16 relapsing. To date, 13 of 18 AML (72%) and 5 of 10 ALL (50%) who were in any CR at transplant, survive disease-free while 4 of the 15 patients (16%) in relapse at transplant survive. The probability of event-free survival for patients transplanted in CR is 60% in the 18 AML patients and 38% in the 10 ALL. The probability of EFS was significantly better in the 16 AML patients whose transplant included donor vs recipient NK cell alloreactivity than in those whose transplant did not (70% vs 7%). In conclusion, given our current results, the most suitable candidate for the full haplotype mismatched transplant should be in early stage disease and selection of an NK alloreactive donor is recommended.
|
['Acute Disease', 'Adolescent', 'Adult', 'Antineoplastic Combined Chemotherapy Protocols', 'Child', 'Disease-Free Survival', 'Haplotypes', 'Histocompatibility', 'Humans', 'Killer Cells, Natural', 'Leukemia', 'Middle Aged', 'Peripheral Blood Stem Cell Transplantation', 'Retrospective Studies', 'Transplantation Conditioning', 'Treatment Outcome', 'Vidarabine', 'Whole-Body Irradiation']
| 12,430,847
|
[['C23.550.291.125'], ['M01.060.057'], ['M01.060.116'], ['E02.183.750.500', 'E02.319.077.500', 'E02.319.310.037'], ['M01.060.406'], ['E01.789.800.190', 'E05.318.740.998.300', 'N04.761.559.590.800.190', 'N05.715.360.575.575.800.190', 'N05.715.360.750.795.300', 'N06.850.520.830.998.300'], ['G05.380.360'], ['G12.875.519'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A11.118.637.555.567.537', 'A15.145.229.637.555.567.537', 'A15.382.490.555.567.537'], ['C04.557.337'], ['M01.060.116.630'], ['E02.095.147.500.500.500.500', 'E04.936.225.687.500.500'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E02.095.465.425.450.800', 'E05.478.610.800'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['D03.633.100.759.590.138.900', 'D13.570.065.950', 'D13.570.583.138.900'], ['E02.815.814', 'E05.980']]
|
['Diseases [C]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Anatomy [A]', 'Chemicals and Drugs [D]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Heparin-induced thrombocytopenia complicated with massive thrombosis of the inferior vena cava after filter placement.
|
A 45-year-old man presented with deep vein thrombosis of the right leg and bilateral pulmonary embolism. Heparin was administered on the initial one and a half days. On the 3rd day, an inferior vena cava (IVC) filter was placed with a heparin flush, after which massive IVC thrombosis developed. The platelet count was 221000/mm3, decreased 42% from the initial level, but remained within the normal range. Heparin was replaced by argatroban on the 13th day. The platelet count increased to 355000/mm3 on the 15th day. The patient was positive for antibody against complexes of heparin and platelet factor 4, and was diagnosed as heparin-induced thrombocytopenia with thrombosis syndrome (HITTS). When thrombosis develops during heparin treatment, it is important to suspect HITTs and to assay for the associated antibodies, regardless of the actual platelet count.
|
['Anticoagulants', 'Femoral Vein', 'Follow-Up Studies', 'Heparin', 'Humans', 'Male', 'Middle Aged', 'Phlebography', 'Popliteal Vein', 'Prosthesis Failure', 'Pulmonary Embolism', 'Thrombocytopenia', 'Tomography, X-Ray Computed', 'Vena Cava Filters', 'Vena Cava, Inferior', 'Venous Thrombosis']
| 16,355,099
|
[['D27.505.954.502.119'], ['A07.015.908.314'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['D09.698.373.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['E01.370.350.700.060.600', 'E01.370.370.050.600'], ['A07.015.908.641'], ['C23.550.767.865', 'E05.325.771'], ['C08.381.746', 'C14.907.355.350.700'], ['C15.378.140.855'], ['E01.370.350.350.810', 'E01.370.350.600.350.700.810', 'E01.370.350.700.700.810', 'E01.370.350.700.810.810', 'E01.370.350.825.810.810'], ['E07.695.207.500'], ['A07.015.908.949.648'], ['C14.907.355.830.925']]
|
['Chemicals and Drugs [D]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Named Groups [M]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Transcriptional and enzymatic profiling of Pleurotus ostreatus laccase genes in submerged and solid-state fermentation cultures.
|
The genome of the white rot basidiomycete Pleurotus ostreatus includes 12 phenol oxidase (laccase) genes. In this study, we examined their expression profiles in different fungal strains under different culture conditions (submerged and solid cultures) and in the presence of a wheat straw extract, which was used as an inducer of the laccase gene family. We used a reverse transcription-quantitative PCR (RT-qPCR)-based approach and focused on determining the reaction parameters (in particular, the reference gene set for the normalization and reaction efficiency determinations) used to achieve an accurate estimation of the relative gene expression values. The results suggested that (i) laccase gene transcription is upregulated in the induced submerged fermentation (iSmF) cultures but downregulated in the solid fermentation (SSF) cultures, (ii) the Lacc2 and Lacc10 genes are the main sources of laccase activity in the iSmF cultures upon induction with water-soluble wheat straw extracts, and (iii) an additional, as-yet-uncharacterized activity (Unk1) is specifically induced in SSF cultures that complements the activity of Lacc2 and Lacc10. Moreover, both the enzymatic laccase activities and the Lacc gene family transcription profiles greatly differ between closely related strains. These differences can be targeted for biotechnological breeding programs for enzyme production in submerged fermentation reactors.
|
['Biotechnology', 'Culture Media', 'Fermentation', 'Fungal Proteins', 'Gene Expression Profiling', 'Gene Expression Regulation, Enzymologic', 'Gene Expression Regulation, Fungal', 'Laccase', 'Mycology', 'Pleurotus', 'Reverse Transcriptase Polymerase Chain Reaction']
| 22,467,498
|
[['H01.158.550', 'J01.897.120'], ['D27.720.470.305', 'E07.206'], ['G02.111.158.249', 'G03.191.249'], ['D12.776.354'], ['E05.393.332'], ['G05.308.320'], ['G05.308.330'], ['D08.811.682.494'], ['H01.158.273.540.553'], ['B01.300.179.100.650'], ['E05.393.620.500.725']]
|
['Disciplines and Occupations [H]', 'Technology, Industry, and Agriculture [J]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
|
An experimental mouse testicular teratoma as a model for neuroepithelial neoplasia and differentiation. II. Electron microscopy.
|
The electron microscopic features of the stages of divergent neuroepithelial differentiation in the solid implants of a transplantable mouse testicular teratoma (OTT-6050) are presented and compared to the sequential stages of cytogenesis that have been described in the developing avian and mammalian central nervous system. Primitive neuroepithelial tumor cells showed the features of undifferentiated multipotential matrix (or ventricular) cells of the neural tube. They formed primitive medullary rosettes, from which various transitions were traced to more differentiated, cilia-containing ependymoblastomatous rosettes; the transitional features included increased granular endoplasmic reticulum and microvilli formation. Glial differentiation was characterized by the presence of mature ependymal rosettes and of astrocytes containing glial filaments. Neuronal differentiation included the development of synapses and the presence of dense-core vesicles in nerve cell processes. No intermediate cell forms were found that suggested multiple lines of differentiation occurring within a single cell.
|
['Animals', 'Cell Differentiation', 'Central Nervous System', 'Male', 'Mice', 'Microscopy, Electron', 'Neoplasm Transplantation', 'Neoplasms, Experimental', 'Neoplasms, Nerve Tissue', 'Neuroectodermal Tumors, Primitive, Peripheral', 'Teratoma', 'Testicular Neoplasms']
| 171,962
|
[['B01.050'], ['G04.152'], ['A08.186'], ['B01.050.150.900.649.313.992.635.505.500'], ['E01.370.350.515.402', 'E05.595.402'], ['E05.624'], ['C04.619', 'E05.598.500.496'], ['C04.557.580'], ['C04.557.465.625.600.590.650', 'C04.557.470.670.590.650', 'C04.557.580.625.600.590.650'], ['C04.557.465.910'], ['C04.588.322.762', 'C04.588.945.440.915', 'C12.294.260.937', 'C12.758.409.937', 'C19.344.762', 'C19.391.829.782']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Antitumor and immunosuppressive activity of Merbarone's analogues and arylidenehydrazinopyrimidines.
|
The synthesis of Merbarone's analogues, the 4-thio- or 4-oxo derivatives of 5-carbamoyl-6-methyl-2-oxo-1,2,3,4-tetrahydropyrimidine is described, starting from 5-carboxy-6-methyl-2-oxo-4-thioxo-1,2,3,4-tetrahydropyrimidine+ ++. That was also used as staring material for synthesis of NCS624947 analogues, the 4-arylidenehydrazino-5-carbamoylpyrimidines. The reduction of arylidenehydrazinopyrimidines with hydrogen on Pd/C was also performed. The obtained compounds were successfully tested for anticancer and immunomodulating activity.
|
['Animals', 'Antibody Formation', 'Antineoplastic Agents', 'Drug Screening Assays, Antitumor', 'Hydrazines', 'Immunoglobulin M', 'Immunosuppressive Agents', 'Magnetic Resonance Spectroscopy', 'Mice', 'Pyrimidines', 'Rats', 'Rats, Sprague-Dawley', 'Sheep', 'Spleen', 'Thiobarbiturates']
| 7,568,316
|
[['B01.050'], ['G12.450.050.370.250'], ['D27.505.954.248'], ['E01.370.225.500.388', 'E05.200.500.388', 'E05.242.417', 'E05.337.550.200'], ['D02.442'], ['D12.776.124.486.485.114.619.574', 'D12.776.124.790.651.114.619.574', 'D12.776.377.715.548.114.619.574'], ['D27.505.696.477.656'], ['E05.196.867.519'], ['B01.050.150.900.649.313.992.635.505.500'], ['D03.383.742'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.750'], ['B01.050.150.900.649.313.500.380.791'], ['A10.549.700', 'A15.382.520.604.700'], ['D03.383.742.698.253.800']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Comparison of Parenteral Opioid Dosing in Adult Sickle Cell Disease Patients With Vaso-occlusive Crisis.
|
Sickle cell disease (SCD) is a chronic condition characterized by multiple vaso-occlusive complications, including acute pain crisis. The mainstay of treatment for patients presenting with vaso-occlusive crisis (VOC) is pain control and adequate hydration. Currently, there are no studies to determine an optimal pain control regimen in adult SCD patients. The main objective of this study is to evaluate whether outcomes differ in patients with VOC based on pain management treatment modality. A retrospective review of admissions with a primary diagnosis of VOC admitted to our facility was conducted. The primary outcome was to compare the average length of stay (LOS) in patients treated with intermittent injection (INT) or patient-controlled analgesia (PCA). Secondary outcomes assessed included 30-day readmission, treatment failure, and impact on pain scores. Of 302 admissions screened, 150 met inclusion criteria (INT: n = 100; PCA: n = 50). Selection of initial pain control regimen showed no difference in average LOS (INT: 5.96 ± 4.19 days vs. PCA: 6.01 ± 3.47 days; P = .94) or 30-day readmission rates (INT: 21% vs. PCA: 16%; P = .52). Treatment failure was significantly higher in the INT group, occurring in 64% of patients vs. 14% in the PCA group (P < .0001). Pain scores were not significantly impacted by selection of pain regimen. Our study indicates that INT and PCA treatment modalities are both effective at controlling pain in VOC; however, more patients in the INT group were characterized as having a treatment failure. Based on our results, it is reasonable to initiate PCA as the primary pain treatment strategy in SCD patients presenting in VOC.
|
['Adult', 'Analgesia, Patient-Controlled', 'Analgesics, Opioid', 'Anemia, Sickle Cell', 'Arterial Occlusive Diseases', 'Cohort Studies', 'Female', 'Hospitalization', 'Humans', 'Length of Stay', 'Male', 'Pain', 'Pain Management', 'Pain Measurement', 'Patient Readmission', 'Retrospective Studies']
| 30,896,312
|
[['M01.060.116'], ['E03.091.120'], ['D27.505.696.277.600.500', 'D27.505.696.663.850.014.760.500', 'D27.505.954.427.040.550.500', 'D27.505.954.427.210.600.500'], ['C15.378.071.141.150.150', 'C15.378.420.155', 'C16.320.070.150', 'C16.320.365.155'], ['C14.907.137'], ['E05.318.372.500.750', 'N05.715.360.330.500.750', 'N06.850.520.450.500.750'], ['E02.760.400', 'N02.421.585.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.760.400.480', 'N02.421.585.400.480'], ['C23.888.592.612', 'F02.830.816.444', 'G11.561.790.444'], ['E02.745', 'N04.590.607.500'], ['E01.370.600.550.324'], ['E02.760.400.620', 'N02.421.585.400.620'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Health Care [N]', 'Organisms [B]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Bronchial intraepithelial neoplastic lesions in head and neck cancer patients: results of autofluorescence bronchoscopy.
|
To determine the efficacy of autofluorescence bronchoscopy for detection of bronchial intraepithelial neoplastic lesions in head and neck cancer patients, we analyzed data from head and neck cancer patients who underwent both white light bronchoscopy and autofluorescence bronchoscopy for the rates of detection of intraepithelial neoplastic lesions. The results of the histopathologic examination were compared with the bronchoscopic findings. The sensitivity for detection of intraepithelial neoplastic lesions was calculated. Eleven moderate dysplasias and 3 severe dysplasias were detected during 8 of the 42 bronchoscopic examinations (19%) in 6 of the 24 patients (25%). The sensitivities for white light bronchoscopy alone and for white light bronchoscopy combined with autofluorescence bronchoscopy for detection of intraepithelial neoplastic lesions were, respectively, 21% (3 of 14) and 57% (8 of 14). In short, bronchial intraepithelial neoplastic lesions were found in a considerable percentage of head and neck cancer patients. Use of autofluorescence bronchoscopy improved the detection of these lesions.
|
['Adult', 'Aged', 'Aged, 80 and over', 'Bronchi', 'Bronchial Neoplasms', 'Bronchoscopy', 'Carcinoma in Situ', 'Carcinoma, Squamous Cell', 'Female', 'Fluorescence', 'Humans', 'Male', 'Middle Aged', 'Neoplasms, Multiple Primary', 'Otorhinolaryngologic Neoplasms', 'Precancerous Conditions']
| 11,465,822
|
[['M01.060.116'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['A04.411.125'], ['C04.588.894.797.520.109', 'C08.127.265', 'C08.785.520.100'], ['E01.370.386.105', 'E01.370.388.250.100', 'E04.502.250.100', 'E04.928.600.080'], ['C04.557.470.200.240'], ['C04.557.470.200.400', 'C04.557.470.700.400'], ['G01.358.500.505.650.665.500', 'G01.590.540.665.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['C04.651'], ['C04.588.443.665', 'C09.647'], ['C04.834']]
|
['Named Groups [M]', 'Anatomy [A]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Adult psychiatric disorder and childhood experiences. The validity of retrospective data.
|
In a sample of young mothers an association was found between a depressed mood and the recall of poor parental relationships during childhood. Women who had been depressed but had recovered by the time of questioning did not recall a poor relationship. A second retrospective measure of a childhood experience, based on more factual details, was unrelated to current mental state.
|
['Adult', 'Depression', 'Female', 'Humans', 'Memory', 'Mental Recall', 'Mothers', 'Parent-Child Relations', 'Pregnancy', 'Pregnancy Complications', 'Research Design', 'Retrospective Studies']
| 6,616,120
|
[['M01.060.116'], ['F01.145.126.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F02.463.425.540'], ['F02.463.425.540.641'], ['F01.829.263.500.320.200', 'I01.880.853.150.500.340.270', 'M01.620.630'], ['F01.829.263.370.290'], ['G08.686.784.769'], ['C13.703'], ['E05.581.500', 'H01.770.644.728'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825']]
|
['Named Groups [M]', 'Psychiatry and Psychology [F]', 'Organisms [B]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Health Care [N]']
| 0
| 1
| 1
| 0
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 1
| 0
|
A novel small-molecule agonist of PPAR-ã potentiates an anti-inflammatory M2 glial phenotype.
|
Neuroinflammation is a key process for many neurodegenerative diseases. Activated microglia and astrocytes play an essential role in neuroinflammation by producing nitric oxide (NO), inflammatory cytokines, chemokines, and neurotoxins. Therefore, targeting glia-mediated neuroinflammation using small-molecules is a potential therapeutic strategy. In this study, we performed a phenotypic screen using microglia cell-based assay to identify a hit compound containing N-carbamoylated urethane moiety (SNU-BP), which inhibits lipopolysaccharide (LPS)-induced NO production in microglia. SNU-BP inhibited pro-inflammatory cytokines and inducible nitric oxide synthase in LPS-stimulated microglia, and potentiated interleukin-4-induced arginase-1 expression. PPAR-ã was identified as a molecular target of SNU-BP. The PPAR response element reporter assay revealed that SNU-BP specifically activated PPAR-ã, but not PPAR-ä or -á, confirming that PPAR-ã is the target protein of SNU-BP. The anti-inflammatory effect of SNU-BP was attenuated by genetic and pharmacological inhibition of PPAR-ã. In addition, SNU-BP induced an anti-inflammatory phenotype in astrocytes as well, by inhibiting pro-inflammatory NO and TNF-á, while increasing anti-inflammatory genes, such as arginase-1 and Ym-1. Finally, SNU-BP exhibited an anti-inflammatory effect in the LPS-injected mouse brain, demonstrating a protective potential for neuroinflammatory diseases.
|
['Animals', 'Anti-Inflammatory Agents', 'Cell Line', 'Cell Survival', 'Dose-Response Relationship, Drug', 'Inflammation Mediators', 'Mice', 'Mice, Inbred C57BL', 'Neuroglia', 'PPAR gamma', 'Phenotype', 'Rats', 'Small Molecule Libraries']
| 27,288,982
|
[['B01.050'], ['D27.505.954.158'], ['A11.251.210'], ['G04.346'], ['G07.690.773.875', 'G07.690.936.500'], ['D23.469'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.420', 'B01.050.150.900.649.313.992.635.505.500.400.420'], ['A08.637', 'A11.650'], ['D12.776.826.239.588'], ['G05.695'], ['B01.050.150.900.649.313.992.635.505.700'], ['D27.720.470.765']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Disturbed gastrointestinal motility and decreased interstitial cells of Cajal in diabetic db/db mice.
|
BACKGROUND AND AIM: Diabetes mellitus (DM) often causes gastrointestinal dysmotility. Interstitial cells of Cajal (ICC), which express c-kit receptor tyrosine kinase (KIT), are considered the pacemaker cells for gastrointestinal movement. The present study was designed to determine the role of ICC in the pathogenesis of gastroenteropathy in type 2 DM.METHODS: We examined C57BL/KsJ-db/db mice as a model for type 2 DM. Gastrointestinal motility was evaluated by measuring gastric emptying, whole gut transit time, and isometric tension of the isolated small intestine. The area of KIT-positive cells in the gastrointestinal tract was examined by image analysis of fluorescent immunohistochemistry. The mRNA expression of KIT ligand, stem cell factor (SCF), in the gastrointestinal tract was quantified by real-time reverse transcription-polymerase chain reaction (RT-PCR).RESULTS: Compared with 12-week-old db/+m control mice, diabetic db/db mice of the same age exhibited delayed gastric emptying, prolonged whole gut transit time, irregular frequency of isometric tension in the small intestine, smaller areas of KIT-positive cells in the antrum, small intestine, and colon, and lower mRNA expression levels of SCF in the small intestine and colon.CONCLUSIONS: We demonstrated disturbed gastrointestinal motility in db/db mice with reduced areas of ICC and expression of SCF. Our results suggest the involvement of ICC in the gastroenteropathy of type 2 DM.
|
['Animals', 'Diabetes Complications', 'Diabetes Mellitus, Type 2', 'Gastrointestinal Diseases', 'Gastrointestinal Motility', 'Male', 'Mice', 'Mice, Inbred C57BL', 'Muscle, Smooth']
| 18,341,539
|
[['B01.050'], ['C19.246.099'], ['C18.452.394.750.149', 'C19.246.300'], ['C06.405'], ['G10.261.360'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.420', 'B01.050.150.900.649.313.992.635.505.500.400.420'], ['A02.633.570', 'A10.690.467']]
|
['Organisms [B]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Anatomy [A]']
| 1
| 1
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
A family with von Hippel-Lindau disease revealed by pheochromocytoma.
|
Von Hippel-Lindau (VHL) disease is an inherited neoplastic disease characterized by a predisposition to develop retinal angiomas, central nervous system hemangioblastomas, renal cell carcinomas, pancreatic cysts and pheochromocytomas. Recently, we encountered three members of the same family who each had both VHL disease and pheochromocytoma. As in all three patients we suspected pheochromocytoma, the diagnosis of VHL disease should be considered. The possible presence of VHL disease was initially investigated in all three patients based on the presence of pheochromocytoma. A mutational analysis of the VHL gene revealed the presence of a missense mutation, consisting of a G to A transversion, at nucleotide 713 in all three patients. This germline point mutation in the VHL gene is often detected in type 2 VHL disease with pheochromocytoma. Genetic analysis seems to be useful for early detection of VHL disease, even when the formal criteria for diagnosis of this disease are lacking.
|
['Adrenal Gland Neoplasms', 'Adult', 'Base Sequence', 'DNA Mutational Analysis', 'Female', 'Humans', 'Ligases', 'Magnetic Resonance Imaging', 'Male', 'Middle Aged', 'Mutation, Missense', 'Pedigree', 'Pheochromocytoma', 'Tomography, X-Ray Computed', 'Tumor Suppressor Proteins', 'Ubiquitin-Protein Ligases', 'Von Hippel-Lindau Tumor Suppressor Protein', 'von Hippel-Lindau Disease']
| 11,510,758
|
[['C04.588.322.078', 'C19.053.347', 'C19.344.078'], ['M01.060.116'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['E05.393.760.700.300'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D08.811.464'], ['E01.370.350.825.500'], ['M01.060.116.630'], ['G05.365.590.650'], ['E05.393.673'], ['C04.557.465.625.650.700.725', 'C04.557.580.625.650.700.725'], ['E01.370.350.350.810', 'E01.370.350.600.350.700.810', 'E01.370.350.700.700.810', 'E01.370.350.700.810.810', 'E01.370.350.825.810.810'], ['D12.776.624.776'], ['D08.811.464.938.750'], ['D08.811.464.938.750.875', 'D12.776.624.776.865'], ['C10.562.925', 'C14.907.077.925', 'C16.131.077.245.750', 'C16.320.184.750']]
|
['Diseases [C]', 'Named Groups [M]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Chemicals and Drugs [D]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 1
| 0
| 0
|
Effect of C/N ratio on extracellular polymeric substances (EPS) and physicochemical properties of activated sludge flocs.
|
The influences of C/N ratio on the extracellular polymeric substances (EPS) and physicochemical properties of the activated sludge flocs were investigated using laboratory-scale sequencing batch reactors (SBRs). Flocs sizes decreased when C/N ratio increased from 20 to 100 and decreased from 20 to 4. The amount of total EPS, TB-EPS, and the carbohydrate and protein contents in TB-EPS were independent of the C/N ratio. In LB-EPS, the protein content increased and the carbohydrate content decreased at decreased C/N ratio, whereas the protein content decreased and the carbohydrate content increased at increased C/N ratio. Effluent suspended solids (ESS) content, turbidity, sludge volume index (SVI), capillary suction time (CST), and specific resistance to filtration (SRF) increased when the C/N ratio decreased, indicating poor flocculation, settleability and dewaterability of the flocs. However, when the C/N ratio increased, only ESS content, SVI and CST value increased. These properties of the flocs were deteriorated greatly under decreased C/N ratio as compared to increased C/N ratio. The characteristics of the flocs could be recovered when C/N ratio returned to the original value. Only the content of protein in LB-EPS was positively correlated with the flocculation, settleability and dewaterability of the flocs.
|
['Carbohydrates', 'Carbon', 'Chemical Phenomena', 'Flocculation', 'Nitrogen', 'Polymers', 'Proteins', 'Sewage', 'Wettability']
| 21,333,444
|
[['D09'], ['D01.268.150'], ['G02'], ['E05.196.150.347', 'G02.159.347'], ['D01.268.604', 'D01.362.625'], ['D05.750', 'D25.720', 'J01.637.051.720'], ['D12.776'], ['D20.944.932.500'], ['G02.409.500', 'G02.860.908']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Technology, Industry, and Agriculture [J]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Clinical Risk Factors Associated With Peripartum Maternal Bacteremia.
|
OBJECTIVE: To evaluate risk factors associated with maternal bacteremia in febrile peripartum women.METHODS: We performed a case-control study of women with fevers occurring between 7 days before and up to 42 days after delivery of viable neonates at two academic hospitals. Women with positive blood cultures were matched with the next two febrile women meeting inclusion criteria with negative blood cultures in the microbiology data without other matching parameters. We compared maternal and neonatal characteristics and outcomes between women in the case group and those in the control group with univariate analysis. We then used logistic regression to examine the association between clinical characteristics and maternal bacteremia.RESULTS: After excluding blood cultures positive only for contaminants, we compared 115 women in the case group with 285 in the control group. Bacteremic women were more likely to experience their initial fever during labor (40.9% compared with 22.8%, P<.01) and more likely to have fever at or above 102°F (62.6% compared with 31.6%, P<.01). These associations persisted in the adjusted analysis: multiparity (adjusted odds ratio [OR] 1.75, 95% CI 1.07-2.87), initial fever during labor (adjusted OR 2.82, 95% CI 1.70-4.70), and fever at or above 102°F (adjusted OR 3.83, 95% CI 2.37-6.19). In an analysis restricted to neonates whose mothers had initial fevers before or in the immediate 24 hours after delivery, neonates born to women in the case group had higher rates of bacteremia compared with those born to women in the control group (9.0% compared with 1.3%, P<.01). Eight of the nine bacteremic neonates born to bacteremic mothers (89%) grew the same organism as his or her mother in blood culture.CONCLUSION: Maternal bacteremia is associated with multiparity, initial fever during labor, and fever at or above 102°F; however, 37.5% of cases of bacteremia occurred in women with maximum fevers below this threshold. Obstetricians should maintain a heightened suspicion for an infectious source of fever in women with these clinical characteristics.
|
['Bacteremia', 'Case-Control Studies', 'Female', 'Fever', 'Humans', 'Infant, Newborn', 'Logistic Models', 'Neonatal Sepsis', 'Obstetric Labor Complications', 'Odds Ratio', 'Peripartum Period', 'Pregnancy', 'Puerperal Infection', 'Risk Factors']
| 28,885,399
|
[['C01.150.252.100', 'C01.757.100', 'C23.550.470.790.500.100'], ['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['C23.888.119.344'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703.520'], ['E05.318.740.500.525', 'E05.318.740.600.800.450', 'E05.318.740.750.450', 'E05.599.835.875', 'N05.715.360.750.530.480', 'N05.715.360.750.625.700.450', 'N05.715.360.750.695.470', 'N06.850.520.830.500.525', 'N06.850.520.830.600.800.450', 'N06.850.520.830.750.450'], ['C01.757.580', 'C16.614.627', 'C23.550.470.790.500.470'], ['C13.703.420'], ['E05.318.740.600.600', 'G17.680.500', 'N05.715.360.750.625.590', 'N06.850.520.830.600.600'], ['G08.686.701'], ['G08.686.784.769'], ['C01.674.715', 'C13.703.700.715', 'C13.703.844.757'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725']]
|
['Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Named Groups [M]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Impact of aortic repair based on flow field computer simulation within the thoracic aorta.
|
Purpose of this computational study is to examine the hemodynamic parameters of velocity fields and shear stress in the thoracic aorta with and without aneurysm, based on an individual patient case and virtual surgical intervention. These two cases, case I (with aneurysm) and II (without aneurysm), are analyzed by computational fluid dynamics. The 3D Navier-Stokes equations and the continuity equation are solved with an unsteady stabilized finite element method. The vascular geometries are reconstructed based on computed tomography angiography images to generate a patient-specific 3D finite element mesh. The input data for the flow waveforms are derived from MR phase contrast flow measurements of a patient before surgical intervention. The computed results show velocity profiles skewed towards the inner aortic wall for both cases in the ascending aorta and in the aortic arch, while in the descending aorta these velocity profiles are skewed towards the outer aortic wall. Computed streamlines indicate that flow separation occurs at the proximal edge of the aneurysm, i.e. computed flow enters the aneurysm in the distal region, and that there is essentially a single, slowly rotating, vortex within the aneurysm during most of the systole. In summary, after virtual surgical intervention in case II higher shear stress distribution along the descending aorta could be found, which may produce more healthy reactions in the endothelium and benefit of vascular reconstruction of an aortic aneurysm at this particular location.
|
['Aorta, Thoracic', 'Aortic Aneurysm, Thoracic', 'Blood Flow Velocity', 'Blood Pressure', 'Computer Simulation', 'Humans', 'Imaging, Three-Dimensional', 'Models, Cardiovascular', 'Radiography', 'Stress, Mechanical']
| 21,316,789
|
[['A07.015.114.056.372'], ['C14.907.055.239.125', 'C14.907.109.139.125'], ['E01.370.370.130', 'G09.330.380.630.080'], ['E01.370.600.875.249', 'G09.330.380.076'], ['L01.224.160'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.350.400', 'L01.224.308.410'], ['E05.599.395.161'], ['E01.370.350.700'], ['G01.374.835']]
|
['Anatomy [A]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Organisms [B]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Simultaneous determination of AZD1152 (prodrug) and AZD1152-hydroxyquinazoline pyrazol anilide by reversed phase liquid chromatography.
|
A simple, selective and sensitive reversed phase liquid chromatography method utilizing ultraviolet detection has been developed and validated for the simultaneous determination of the prodrug AZD1152 and its active product AZD1152-hydroxyquinazoline pyrazol anilide (hQPA) in human and mouse plasma and mouse tissues. Isocratic separation was achieved using an 5mum UptiSphere HDO C-18 column (150 mm x 4.6 mm) with guard column in combination with a mobile phase comprised of phosphate buffered water (50 mM; pH 3.4) and acetonitrile (81.5: 18.5; v/v). UV detection at 318 nm was used. Sample preparation involved a single-step protein precipitation with ethanol. Ex vivo conversion of AZD1152 by endogenous phosphatases was prevented by immediate cooling of the samples in ice-water and addition of sodium fluoride and EDTA. The validation parameters included specificity, recovery, accuracy, precision, sensitivity and stability. The lower limit of quantification in human plasma for AZD1152 and hQPA was 25 ng/ml. The applicability of the method was demonstrated by successful determination of AZD1152 and hQPA in human plasma and in plasma, brain, liver, kidney and ileum samples from mice dosed with AZD1152.
|
['Animals', 'Chromatography, High Pressure Liquid', 'Humans', 'Hydroxyquinolines', 'Mice', 'Molecular Structure', 'Organophosphates', 'Pyrazoles', 'Quinazolines', 'Reproducibility of Results']
| 19,744,901
|
[['B01.050'], ['E05.196.181.400.300'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D03.633.100.810.350'], ['B01.050.150.900.649.313.992.635.505.500'], ['G02.111.570', 'G02.466'], ['D02.705.400'], ['D03.383.129.539'], ['D03.633.100.786'], ['E05.318.370.725', 'E05.337.851', 'N05.715.360.325.685', 'N06.850.520.445.725']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Health Care [N]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
The hospital as a work of art: one hundred thirty years of dermatology in Dresden-Friedrichstadt.
|
The department of dermatology at the Hospital Dresden-Friedrichstadt (Dresden, Germany) was founded in 1874 as one of the oldest departments of dermatology in a municipal hospital in Germany. Dresden was the capital of Saxony and, as such, one of the most influential cultural centers of Germany. This particular situation is also reflected by the hospital's history itself.
|
['Art', 'Dermatology', 'Germany', 'History, 18th Century', 'History, 19th Century', 'History, 20th Century', 'Hospital Departments', 'Hospitals, Municipal', 'Humans', 'Models, Anatomic']
| 18,280,908
|
[['K01.093'], ['H02.403.225'], ['Z01.542.315'], ['K01.400.504.875'], ['K01.400.504.937'], ['K01.400.504.968'], ['N02.278.216.500.968', 'N04.452.442.452.422'], ['N02.278.421.510.210', 'N02.278.421.660.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['J01.897.280.500.545.129', 'L01.178.820.090.545.129']]
|
['Humanities [K]', 'Disciplines and Occupations [H]', 'Geographicals [Z]', 'Health Care [N]', 'Organisms [B]', 'Technology, Industry, and Agriculture [J]', 'Information Science [L]']
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 1
|
Multivitamin supplementation of adult omnivores and lactovegetarians: circulating levels of vitamin A, D and E, lipids, apolipoproteins and selenium.
|
Serum levels of fat-soluble vitamins, lipids, apolipoproteins, total protein, hemoglobin, iron, and selenium were determined in healthy Finnish adults during a 7-month period beginning in January and ending in August. The subjects were either omnivores or established lactovegetarians, who had consumed their respective diets for at least 6 months prior to the study. Half of the subjects in both groups received daily multivitamin supplementation and the other half served as controls. In the beginning, the lactovegetarians differed from the omnivores in having lower serum levels of protein, apolipoproteins A-I and C-II, and higher levels of standardized alpha-tocopherol. During the study, serum retinol and standardized alpha-tocopherol (in March and May), as well as apolipoproteins A-I and C-II, and selenium decreased in the omnivores and 25-hydroxyvitamin D2, 25-hydroxyvitamin D3, cholesterol, HDL-cholesterol, and the HDL-cholesterol/cholesterol ratio increased. Apolipoprotein B decreased and then increased. In the lactovegetarians, serum selenium and protein decreased during the study, whereas retinol and alpha-tocopherol stayed higher than in the omnivores. Consumption of the lactovegetarian diet was accompanied by lower circulating levels of cholesterol and selenium and higher levels of retinol and standardized alpha-tocopherol than the mixed diet. Multivitamin supplementation may have value especially for omnivores in northern countries, like Finland, in providing better retinol, alpha-tocopherol, vitamin D, and selenium status in late winter and early spring.
|
['Adult', 'Apolipoprotein A-I', 'Apolipoprotein C-II', 'Apolipoproteins', 'Apolipoproteins A', 'Apolipoproteins B', 'Apolipoproteins C', 'Cholesterol, HDL', 'Diet, Vegetarian', 'Eating', 'Female', 'Humans', 'Lipid Metabolism', 'Lipids', 'Male', 'Nutritive Value', 'Selenium', 'Triglycerides', 'Vitamin A', 'Vitamin D', 'Vitamin E', 'Vitamins']
| 2,117,596
|
[['M01.060.116'], ['D10.532.091.200.100', 'D12.776.070.400.200.100', 'D12.776.521.120.200.100'], ['D10.532.091.400.750', 'D12.776.070.400.400.750', 'D12.776.521.120.400.750'], ['D10.532.091', 'D12.776.070.400', 'D12.776.521.120'], ['D10.532.091.200', 'D12.776.070.400.200', 'D12.776.521.120.200'], ['D10.532.091.300', 'D12.776.070.400.300', 'D12.776.521.120.300'], ['D10.532.091.400', 'D12.776.070.400.400', 'D12.776.521.120.400'], ['D04.210.500.247.808.197.238', 'D10.532.432.400', 'D10.570.938.208.270', 'D12.776.521.479.470'], ['E02.642.249.300', 'G07.203.650.240.300'], ['G07.203.650.283', 'G10.261.330'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G03.458'], ['D10'], ['G07.203.650.660', 'J01.576.423.850.730.750', 'N06.850.601.750'], ['D01.268.185.850', 'D01.578.700'], ['D10.351.801'], ['D02.455.326.271.665.202.495.818', 'D02.455.426.392.368.367.379.249.700.860', 'D02.455.849.131.495.818', 'D02.455.849.291.925', 'D23.767.261.700.860'], ['D04.210.500.812.768'], ['D03.383.663.283.909', 'D03.633.100.150.909'], ['D27.505.696.494.600', 'G07.203.300.681.500.600', 'J02.500.681.500.600']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Technology, Industry, and Agriculture [J]', 'Health Care [N]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 1
| 0
|
Identification and functional annotation of metabolism-associated lncRNAs and their related protein-coding genes in gastric cancer.
|
BACKGROUND: Long noncoding RNAs (lncRNAs) play important roles in carcinogenesis. However, the roles of metabolism-associated lncRNAs in cancers are still unclear.METHODS: A microarray of metabolism-associated lncRNAs was used to detect their expression patterns between gastric cancer and paired nontumorous tissues. Its results and gastric cancer differential gene expression data from public databases were used to screen the metabolic pathway-associated lncRNAs. A metabolic network with microRNAs (miRNAs), lncRNAs, and protein-coding genes was further constructed. Finally, the expression of TOPORS antisense RNA 1 (TOPORS-AS1), a screened highly expressed lncRNA and its associated protein-coding gene, NADH: ubiquinone oxidoreductase subunit B6 (NDUFB6), were verified by reverse transcription polymerase chain reaction.RESULTS: A total of eight upregulated and one downregulated lncRNAs and 25 upregulated and 20 downregulated protein-coding genes were found to be involved in metabolism in gastric cancer. Within the lncRNAs-miRNAs-mRNAs metabolic network, 78 miRNA-target links, 546 positive coexpression relationships, and 191 protein-protein interactions were found. The expression of TOPORS-AS1 and its associated gene, NDUFB6 in gastric cancer tissues was significantly lower than that in adjacent nontumor tissues. Moreover, NDUFB6 expression was associated with the invasion and distal metastasis of gastric cancer.CONCLUSIONS: The metabolism-associated lncRNAs play important roles in the occurrence of gastric cancer.
|
['Female', 'Gene Expression Regulation, Neoplastic', 'Gene Regulatory Networks', 'Humans', 'Male', 'Neoplasm Invasiveness', 'Neoplasm Metastasis', 'Neoplasm Proteins', 'RNA, Long Noncoding', 'RNA, Neoplasm', 'Stomach Neoplasms']
| 29,992,774
|
[['G05.308.370'], ['G05.360.080.689.360'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C04.697.645', 'C23.550.727.645'], ['C04.697.650', 'C23.550.727.650'], ['D12.776.624'], ['D13.444.735.790.375'], ['D13.444.735.615'], ['C04.588.274.476.767', 'C06.301.371.767', 'C06.405.249.767', 'C06.405.748.789']]
|
['Phenomena and Processes [G]', 'Organisms [B]', 'Diseases [C]', 'Chemicals and Drugs [D]']
| 0
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Direct observation of catalytic oxidation of particulate matter using in situ TEM.
|
The ability to observe chemical reactions at the molecular level convincingly demonstrates the physical and chemical phenomena occurring throughout a reaction mechanism. Videos obtained through in situ transmission electron microscopy (TEM) revealed the oxidation of catalytic soot under practical reaction conditions. Carbon oxidation reactions using Ag/SiO2 or Cs2CO3/nepheline catalysts were performed at 330 °C under an O2 flow of 0.5 Pa in the TEM measurement chamber. Ag/SiO2 catalyzed the reaction at the interface of the mobile Ag species and carbon, while the Cs species was fixed on the nepheline surface during the reaction. In the latter case, carbon particles moved, remained attached to the Cs2CO3/nepheline surface, and were consumed at the interface by the oxidation reaction. Using this technique, we were able to visualize such mobile and immobile catalysis according to different mechanisms.
|
['Carbon', 'Catalysis', 'Microscopy, Electron, Transmission', 'Oxidation-Reduction', 'Particulate Matter', 'Silicon Dioxide', 'Silver']
| 26,154,580
|
[['D01.268.150'], ['G02.130'], ['E01.370.350.515.402.580', 'E05.595.402.580'], ['G02.700', 'G03.295.531'], ['D20.633'], ['D01.578.750', 'D01.650.550.825', 'D01.837.725'], ['D01.268.556.812', 'D01.268.956.843', 'D01.552.544.812']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Usability analysis of the tele-nursing call management software at HealthLink BC.
|
Usability engineering methods have been shown to be effective in identifying software problems that may lead to user operating inefficiencies, user errors, data encoding errors or far more serious health threatening consequences. This research project applied two complementary usability engineering analysis methods to a mature tele-nursing clinical call management software platform (a knowledgebase and an EMR product). Findings from the study revealed 100 discrete usability errors or problems. This research also introduced an adaptation of cognitive task analysis, with the development of a 'cognitive task screen-turn' analysis, which provided useful information about operating differences among users performing identical tasks that was particularly useful in revealing four unnecessary steps within the system.
|
['British Columbia', 'Electronic Health Records', 'Humans', 'Software', 'Telenursing', 'User-Computer Interface']
| 21,335,712
|
[['Z01.107.567.176.160'], ['E05.318.308.940.968.625.500', 'N04.452.859.564.650.125', 'N05.715.360.300.715.500.530.250', 'N06.850.520.308.940.968.625.250'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['L01.224.900'], ['N04.452.758.377.937'], ['L01.224.900.910']]
|
['Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Information Science [L]']
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 1
| 1
|
Interactions of membrane-active peptides with thick, neutral, nonzwitterionic bilayers.
|
Alamethicin is a well-studied channel-forming peptide that has a prototypical amphipathic helix structure. It permeabilizes both microbial and mammalian cell membranes, causing loss of membrane polarization and leakage of endogenous contents. Antimicrobial peptide-lipid systems have been studied quite extensively and have led to significant advancements in membrane biophysics. These studies have been performed on lipid bilayers that are generally charged or zwitterionic and restricted to a thickness range of 3-5 nm. Bilayers of amphiphilic diblock copolymers are a relatively new class of membranes that can have significantly different physicochemical properties compared with those of lipid membranes. In particular, they can be made uncharged, nonzwitterionic, and much thicker than their lipid counterparts. In an effort to extend studies of membrane-protein interactions to these synthetic membranes, we have characterized the interactions of alamethicin and several other membrane-active peptides with diblock copolymer bilayers. We find that although alamethicin is too small to span the bilayer, the peptide interacts with, and ruptures, thick polymer membranes.
|
['Alamethicin', 'Circular Dichroism', 'Fluoresceins', 'Hydrophobic and Hydrophilic Interactions', 'Spectrometry, Fluorescence', 'Water']
| 16,852,806
|
[['D04.345.566.040', 'D12.644.504.111', 'D12.644.641.040'], ['E05.196.867.151'], ['D02.455.426.779.347', 'D03.633.300.953.275', 'D04.711.347'], ['G02.409'], ['E05.196.712.516.600.676', 'E05.196.867.726'], ['D01.045.250.875', 'D01.248.497.158.459.650', 'D01.650.550.925']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Genome-Wide Analysis of Head and Neck Squamous Cell Carcinomas Reveals HPV, TP53, Smoking and Alcohol-Related Allele-Based Acquired Uniparental Disomy Genomic Alterations.
|
Smoking and alcohol intake are major risk factors in head and neck squamous cell carcinomas (HNSCCs). Although the link between TP53 mutation and smoking has been well established, very little is known about the link between acquired uniparental disomy (aUPD) and smoking and/or alcohol consumption or other clinical characteristics. We used TCGA genomic data to investigate whether smoking, alcohol intake, clinical and demographic variables, HPV status and TP53 mutation are associated with aUPD at specific chromosomal regions. In multivariate analysis, we found association between aUPD regions and risk factors and clinical variables of disease. aUPD regions on chromosome 4q, 5q, 9p, 9q, 13q, 17p and CDKN2A occurred significantly more often in patients with TP53-mutated HNSCC than in those with wild-type HNSCC, while aUPD regions on chromosome 9p and at CDKN2A were significantly more frequent in females than in males. Besides, aUPD occurred more frequent in HPV-positive than in HPV-negative samples with all HNSCC and larynx cancers on chromosome 9q 15q and 17p. Moreover, aUPD on CDKN2A region occurred more often in alcohol drinkers than nondrinkers in patients with all HNSCC and oral cavity cancers, while aUPD region on chromosome 5q occurred less in alcohol drinkers than nondrinkers in patients with all HNSCC and oral cavity cancers. Similarly, aUPD region on chromosome 5q occurred less in smokers than nonsmokers in patients with all HNSCC and oral cavity cancers. In conclusion, aUPD regions are not random, and certain regions are associated with risk factors for disease, and with TP53 mutation status.
|
['Alcohol Drinking', 'Alleles', 'Databases, Genetic', 'Genome-Wide Association Study', 'Humans', 'Mutation', 'Neoplasm Grading', 'Neoplasm Staging', 'Papillomavirus Infections', 'Polymorphism, Single Nucleotide', 'Smoking', 'Squamous Cell Carcinoma of Head and Neck', 'Tumor Suppressor Protein p53', 'Uniparental Disomy']
| 30,616,092
|
[['F01.145.317.269'], ['G05.360.340.024.340.030'], ['L01.313.500.750.300.188.400.325', 'L01.470.750.750.325'], ['E05.318.370.392', 'E05.318.416.249', 'E05.393.385.500', 'E05.393.522.500', 'E05.393.760.640.500', 'N06.850.520.445.392', 'N06.850.520.470.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G05.365.590'], ['E01.789.612'], ['E01.789.625'], ['C01.925.256.650', 'C01.925.928.725'], ['G05.365.795.598'], ['F01.145.805'], ['C04.557.470.200.400.565', 'C04.588.443.177'], ['D12.776.157.687.650', 'D12.776.260.820', 'D12.776.624.776.775', 'D12.776.660.720.650', 'D12.776.744.845'], ['C23.550.210.645.890', 'G05.365.590.175.935']]
|
['Psychiatry and Psychology [F]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Diseases [C]', 'Chemicals and Drugs [D]']
| 0
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 1
| 0
| 1
| 0
|
Production characters of straightbred, F1 and F2 cows: birth and weaning characters of terminal-cross calves.
|
Records of 2,449 births and 2,120 weanings of terminal-cross calves were used to characterize maternal productivity of first- and second-generation cows from a diallel of Angus, Brahman, Hereford, Holstein and Jersey when mated to third-breed sires. Third- and later-parity cows were randomly assigned after each parturition to Charolais and Red Poll bulls in multiple-sire pastures. Calves were weaned at approximately 7 mo of age; males were not castrated. A mixed model was assumed for data analysis. Effects included in the model were breed-type of dam, cow within breed-type of dam (random), breed of sire of calf, season of record, year of record, age of dam group, sex of calf and age of calf (covariate). Age of dam groups were 4- and 5-yr-olds, 6- and 7-yr-olds, 8-, 9- and 10-yr-olds, and those greater than 10 yr of age. Dependent variables were calf weight, shoulder width and hip width at birth, weaning weight, weaning height and survival to weaning. Holstein and Holstein crosses tended to produce the largest calves at birth and weaning. Among straightbred dams, the smallest calves were born to Brahman, whereas Hereford weaned the smallest calves. Brahman-Jersey dams produced the smallest calves at birth among crossbreds; Angus-Hereford cows weaned the smallest calves. Average maternal heterosis estimates for birth weight were small and non-significant. Calves of F1 crossbred dams were 17.4 kg heavier (P less than .01) and 1.70 cm taller (P less than .01) at weaning than calves of first-generation straightbred dams.(ABSTRACT TRUNCATED AT 250 WORDS)
|
['Animals', 'Animals, Newborn', 'Birth Weight', 'Breeding', 'Cattle', 'Crosses, Genetic', 'Female', 'Weaning']
| 2,793,620
|
[['B01.050'], ['B01.050.050.282'], ['C23.888.144.186', 'E01.370.600.115.100.160.120.186', 'E05.041.124.160.750.149', 'G07.100.100.160.120.186', 'G07.345.249.314.120.186'], ['E05.820.150', 'G05.090'], ['B01.050.150.900.649.313.500.380.271'], ['E05.393.281'], ['G07.203.650.220.500.750', 'G07.203.650.915']]
|
['Organisms [B]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Therapeutic adherence in patients with systemic lupus erythematosus: a cross-sectional study.
|
INTRODUCTION: The aim of the research was the study of the adherence to treatment in patients with systemic lupus erythematosus.METHODS: Cross-sectional study including 132 consecutive patients with systemic lupus erythematosus (SLICC, 2012 classification criteria). We collected clinical and socio-demographic data, socio-economic status; we assessed SLEDAI-2k disease activity, and estimated the adherence to treatment by Morisky questionnaire.RESULTS: Our results demonstrated that low adherence to treatment in patients with systemic lupus erythematosus was in only 11.36% of patients, while 43.18% and 45.46% of the patients were scored as moderate and high adherence, respectively. A moderate/high adherence to treatment was associated to a high level of education (r = -0.51, p < 0.05, 95% CI = -0.25 to -0.66), low disease activity (r = 0.38, p < 0.05, 95% CI = 0.25 to 0.53) and low indices of physician global assessment (r = -0.31, p<0.05, 95% CI = -0.23 to -0.71). The sub-analysis of the adherence to each drug demonstrated that the highest adherence was to treatment with glucocorticosteroids - 92.85%, followed by hydroxychloroquine and aspirin - 92.15% and 89.79%, respectively.CONCLUSION: In our cohort, the adherence to treatment was high in 45.46%, moderate in 43.18% and low in only 11.36% cases. High adherence to treatment was associated to low disease activity. The adherence was positively influenced by the age at the onset of the disease and a high educational level.
|
['Adolescent', 'Adult', 'Age of Onset', 'Aged', 'Anti-Inflammatory Agents, Non-Steroidal', 'Aspirin', 'Cross-Sectional Studies', 'Educational Status', 'Employment', 'Female', 'Glucocorticoids', 'Humans', 'Hydroxychloroquine', 'Immunosuppressive Agents', 'Income', 'Lupus Erythematosus, Systemic', 'Male', 'Medication Adherence', 'Middle Aged', 'Surveys and Questionnaires', 'Young Adult']
| 29,427,555
|
[['M01.060.057'], ['M01.060.116'], ['N05.715.350.075.100', 'N06.850.490.250.100'], ['M01.060.116.100'], ['D27.505.696.663.850.014.040.500', 'D27.505.954.158.030', 'D27.505.954.329.030'], ['D02.455.426.559.389.657.410.595.176'], ['E05.318.372.500.875', 'N05.715.360.330.500.875', 'N06.850.520.450.500.875'], ['N01.824.196'], ['N01.824.245'], ['D06.472.040.543', 'D27.505.696.399.472.488'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D03.633.100.810.050.180.350'], ['D27.505.696.477.656'], ['N01.824.417'], ['C17.300.480', 'C20.111.590'], ['F01.100.150.750.500.600.500', 'F01.145.488.887.500.600.500', 'N05.300.150.800.500.600.500'], ['M01.060.116.630'], ['E05.318.308.980', 'N05.715.360.300.800', 'N06.850.520.308.980'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Health Care [N]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Diseases [C]', 'Psychiatry and Psychology [F]']
| 0
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Morphometric analysis of the relationships between intervertebral disc and vertebral body heights: an anatomical and radiographic study of the human thoracic spine.
|
The main aim of this study was to provide anatomical data on the heights of the human intervertebral discs for all levels of the thoracic spine by direct and radiographic measurements. Additionally, the heights of the neighboring vertebral bodies were measured, and the prediction of the disc heights based only on the size of the vertebral bodies was investigated. The anterior (ADH), middle (MDH) and posterior heights (PDH) of the discs were measured directly and on radiographs of 72 spine segments from 30 donors (age 57.43 ± 11.27 years). The radiographic measurement error and the reliability of the measurements were calculated. Linear and non-linear regression analyses were employed for investigation of statistical correlations between the heights of the thoracic disc and vertebrae. Radiographic measurements displayed lower repeatability and were shorter than the anatomical ones (approximately 9% for ADH and 37% for PDH). The thickness of the discs varied from 4.5 to 7.2 mm, with the MDH approximately 22.7% greater. The disc heights showed good correlations with the vertebral body heights (R(2), 0.659-0.835, P-values < 0.005; anova), allowing the generation of 10 prediction equations. New data on thoracic disc morphometry were provided in this study. The generated set of regression equations could be used to predict thoracic disc heights from radiographic measurement of the vertebral body height posterior. For the creation of parameterized models of the human thoracic discs, the use of the prediction equations could eliminate the need for direct measurement on intervertebral discs. Moreover, the error produced by radiographic measurements could be reduced at least for the PDH.
|
['Adult', 'Aged', 'Aged, 80 and over', 'Body Height', 'Female', 'Humans', 'Intervertebral Disc', 'Male', 'Middle Aged', 'Observer Variation', 'Radiography', 'Reproducibility of Results', 'Thoracic Vertebrae']
| 21,615,399
|
[['M01.060.116'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['E01.370.600.115.100.160.100', 'E05.041.124.160.500', 'G07.100.100.160.100', 'G07.345.249.314.100'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A02.165.308.410', 'A02.835.232.834.432', 'A10.165.382.350.050'], ['M01.060.116.630'], ['E01.354.753', 'N02.421.450.600', 'N05.715.350.150.675', 'N06.850.490.500.250'], ['E01.370.350.700'], ['E05.318.370.725', 'E05.337.851', 'N05.715.360.325.685', 'N06.850.520.445.725'], ['A02.835.232.834.892']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Anatomy [A]', 'Health Care [N]']
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Determination of the primary structure of PLC-154 demonstrates diversity of phosphoinositide-specific phospholipase C activities.
|
Protein sequence analysis of a bovine brain phosphoinositide-specific phospholipase C (PI-PLC; PLC-154) has permitted the isolation of a cDNA that appears to code for this protein. Transient expression of this cDNA in COS-1 cells demonstrates that the cDNA encodes a functional phospholipase C that migrates at approximately 150,000 daltons. A transcript of approximately 7 kb is observed in RNA derived from bovine brain and a related transcript of the same size is present in certain human cell lines. Southern blot analysis indicates that one or possibly two genes hybridize with a PLC-154 probe. Regions of homology between PLC-154 and the previously described PLC-148 allow the assignment of a putative catalytic domain to the central region of PLC-154.
|
['Amino Acid Sequence', 'Animals', 'Base Sequence', 'Brain', 'Cattle', 'Cell Line', 'Cloning, Molecular', 'DNA', 'Electrophoresis, Polyacrylamide Gel', 'Gene Expression Regulation', 'Molecular Sequence Data', 'Nucleic Acid Hybridization', 'Phosphatidylinositols', 'RNA', 'Sequence Homology, Nucleic Acid', 'Substrate Specificity', 'Transcription, Genetic', 'Type C Phospholipases']
| 2,455,601
|
[['G02.111.570.060', 'L01.453.245.667.060'], ['B01.050'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['A08.186.211'], ['B01.050.150.900.649.313.500.380.271'], ['A11.251.210'], ['E05.393.220'], ['D13.444.308'], ['E05.196.401.402', 'E05.301.300.319'], ['G05.308'], ['L01.453.245.667'], ['E05.393.661', 'G02.111.611'], ['D10.570.755.375.760.400.942'], ['D13.444.735'], ['G02.111.810.550', 'G05.810.550'], ['G02.111.835'], ['G02.111.873', 'G05.297.700'], ['D08.811.277.352.640.700.700']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Organisms [B]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Statistical aspects of quantitative image analysis of beta-amyloid in the APP(V717F) transgenic mouse model of Alzheimer's disease.
|
Cerebral beta-amyloidosis is a central part of the neuropathology of Alzheimer's disease (AD). Quantitation of beta-amyloid plaques in the human AD brain, and in animal models of AD, is an important study endpoint in AD research. Methodologic approaches to the measurement of beta-amyloid in the brain vary between investigators, and these differences affect outcome measures. Here, one quantitative approach to the measurement of beta-amyloid plaques in brain sections was analyzed for sources of variability due to sampling. Brain tissue was from homozygous APP(V717F) transgenic male mice. Sampling variables were at the mouse and microscopic slide and field levels. Results indicated that phenotypic variability in the mouse sample population was the largest contributor to the standard error of the analyses. Within each mouse, variability between slides or between fields within slides had smaller effects on the error of the analyses. Therefore, when designing studies of adequate power, in this and in other similar models of cerebral beta-amyloidosis, sufficient numbers of mice per group must be included in order for change in mean plaque burden attributable to an experimental variable to outweigh phenotypic variability.
|
['Alzheimer Disease', 'Amyloid beta-Peptides', 'Amyloid beta-Protein Precursor', 'Animals', 'Benzothiazoles', 'Cell Count', 'Data Interpretation, Statistical', 'Disease Models, Animal', 'Hippocampus', 'Image Processing, Computer-Assisted', 'Male', 'Mice', 'Mice, Transgenic', 'Microscopy, Fluorescence', 'Plaque, Amyloid', 'Reproducibility of Results', 'Statistical Distributions', 'Thiazoles']
| 11,478,973
|
[['C10.228.140.380.100', 'C10.574.945.249', 'F03.615.400.100'], ['D12.644.024', 'D12.776.049.407.249.500', 'D12.776.543.039.500'], ['D12.776.049.407.249', 'D12.776.543.039', 'D12.776.645.468.500', 'D12.776.811.050'], ['B01.050'], ['D03.383.129.708.089', 'D03.633.100.185'], ['E01.370.225.500.195', 'E05.200.500.195', 'E05.242.195', 'G04.140'], ['E05.245.380', 'E05.318.740.300', 'L01.313.500.750.190.380', 'N05.715.360.750.300', 'N06.850.520.830.300'], ['C22.232', 'E05.598.500', 'E05.599.395.080'], ['A08.186.211.180.405', 'A08.186.211.200.885.287.500.345'], ['L01.224.308'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.136.500', 'B01.050.150.900.649.313.992.635.505.500.800'], ['E01.370.350.515.458', 'E05.595.458'], ['C23.300.821'], ['E05.318.370.725', 'E05.337.851', 'N05.715.360.325.685', 'N06.850.520.445.725'], ['E05.318.740.994', 'G17.820', 'N05.715.360.750.750', 'N06.850.520.830.994'], ['D02.886.675', 'D03.383.129.708']]
|
['Diseases [C]', 'Psychiatry and Psychology [F]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Health Care [N]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 1
| 0
| 1
| 0
|
The prospective association between sleep deprivation and depression among adolescents.
|
STUDY OBJECTIVES: To examine the prospective, reciprocal association between sleep deprivation and depression among adolescents.DESIGN: A community-based two-wave cohort study.SETTING: A metropolitan area with a population of over 4 million.PARTICIPANTS: 4,175 youths 11-17 at baseline, and 3,134 of these followed up a year later.MEASUREMENTS: Depression is measured using both symptoms of depression and DSM-IV major depression. Sleep deprivation is defined as ? 6 h of sleep per night.RESULTS: Sleep deprivation at baseline predicted both measures of depression at follow-up, controlling for depression at baseline. Examining the reciprocal association, major depression at baseline, but not symptoms predicted sleep deprivation at follow-up.CONCLUSION: These results are the first to document reciprocal effects for major depression and sleep deprivation among adolescents using prospective data. The data suggest reduced quantity of sleep increases risk for major depression, which in turn increases risk for decreased sleep.
|
['Adolescent', 'Child', 'Cohort Studies', 'Depression', 'Depressive Disorder, Major', 'Diagnostic and Statistical Manual of Mental Disorders', 'Female', 'Humans', 'Male', 'Odds Ratio', 'Prospective Studies', 'Risk', 'Sleep', 'Sleep Deprivation', 'Texas']
| 24,497,652
|
[['M01.060.057'], ['M01.060.406'], ['E05.318.372.500.750', 'N05.715.360.330.500.750', 'N06.850.520.450.500.750'], ['F01.145.126.350'], ['F03.600.300.375'], ['L01.453.245.945.200'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.740.600.600', 'G17.680.500', 'N05.715.360.750.625.590', 'N06.850.520.830.600.600'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['E05.318.740.600.800', 'G17.680.750', 'N05.715.360.750.625.700', 'N06.850.520.830.600.800'], ['F02.830.855', 'G11.561.803'], ['C10.886.425.175', 'C23.888.592.796.772', 'F02.830.855.671', 'F03.870.400.099'], ['Z01.107.567.875.760.750']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Psychiatry and Psychology [F]', 'Information Science [L]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Geographicals [Z]']
| 0
| 1
| 1
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 1
| 1
| 1
| 1
|
Priming of neutrophil respiratory burst activity by lipopolysaccharide from Burkholderia cepacia.
|
Neutrophil activation may play an important role in the pathogenesis of respiratory disease in Burkholderia cepacia-colonized cystic fibrosis (CF) patients. As bacterial lipopolysaccharides (LPS) are potent immunostimulatory molecules, we investigated the role of B. cepacia LPS in neutrophil activation processes. LPS extracted from a highly transmissible and virulent strain of B. cepacia (J2315) was found to increase neutrophil surface expression of the beta2 integrin, complement receptor 3, and to prime neutrophil respiratory burst responses to the neutrophil-activating agent fMet-Leu-Phe. By contrast, LPS extracted from a nonmucoid Pseudomonas aeruginosa strain isolated from a patient with CF showed little or no priming activity. As B. cepacia is currently being developed as a biocontrol agent for large-scale agricultural release, we compared LPS molecules from a range of bacterial strains for their proinflammatory ability. Priming activity was demonstrated in LPS extracts from all B. cepacia strains tested, with one environmental strain, J2552, showing the highest activity. These findings indicate (i) that B. cepacia LPS may contribute to the inflammatory nature of B. cepacia infection in CF patients, both by promoting increased neutrophil recruitment and by priming neutrophil respiratory burst responses, and (ii) that environmental strains of B. cepacia may have considerable inflammatory potential in susceptible individuals.
|
['Burkholderia cepacia', 'Cystic Fibrosis', 'Escherichia coli', 'Humans', 'Hydrogen Peroxide', 'Lipopolysaccharides', 'Macrophage-1 Antigen', 'N-Formylmethionine Leucyl-Phenylalanine', 'Neutrophil Activation', 'Neutrophils', 'Pseudomonas Infections', 'Pseudomonas aeruginosa', 'Respiratory Burst', 'Species Specificity']
| 9,317,038
|
[['B03.660.075.090.688.100.110.500'], ['C06.689.202', 'C08.381.187', 'C16.320.190', 'C16.614.213'], ['B03.440.450.425.325.300', 'B03.660.250.150.180.100'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D01.248.497.158.685.750.424', 'D01.339.431.374.424', 'D01.650.550.750.400', 'D02.389.338.253'], ['D09.400.500', 'D09.698.718.450', 'D10.494', 'D23.050.161.616.525', 'D23.946.123.329.500'], ['D12.776.543.750.705.408.495.500', 'D12.776.543.750.705.408.600.500', 'D12.776.543.750.705.833.500'], ['D02.886.030.676.450.440', 'D12.125.072.050.685.445', 'D12.125.142.666.500', 'D12.125.166.676.450.440', 'D12.644.456.400', 'D23.125.685'], ['G12.604'], ['A11.118.637.415.583', 'A11.627.340.583', 'A11.733.689', 'A15.145.229.637.415.583', 'A15.382.490.315.583', 'A15.382.680.689'], ['C01.150.252.400.739'], ['B03.440.400.425.625.625.100', 'B03.660.250.580.590.050'], ['G03.197.620', 'G04.270.620'], ['G16.824']]
|
['Organisms [B]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Reactivation of flagellar motility in demembranated Leishmania reveals role of cAMP in flagellar wave reversal to ciliary waveform.
|
The flagellum of parasitic trypanosomes is a multifunctional appendage essential for its viability and infectivity. However, the biological mechanisms that make the flagellum so dynamic remains unexplored. No method is available to access and induce axonemal motility at will to decipher motility regulation in trypanosomes. For the first time we report the development of a detergent-extracted/demembranated ATP-reactivated model for studying flagellar motility in Leishmania. Flagellar beat parameters of reactivated parasites were similar to live ones. Using this model we discovered that cAMP (both exogenous and endogenous) induced flagellar wave reversal to a ciliary waveform in reactivated parasites via cAMP-dependent protein kinase A. The effect was reversible and highly specific. Such an effect of cAMP on the flagellar waveform has never been observed before in any organism. Flagellar wave reversal allows parasites to change direction of swimming. Our findings suggest a possible cAMP-dependent mechanism by which Leishmania responds to its surrounding microenvironment, necessary for its survival. Our demembranated-reactivated model not only serves as an important tool for functional studies of flagellated eukaryotic parasites but has the potential to understand ciliary motility regulation with possible implication on human ciliopathies.
|
['Axoneme', 'Cyclic AMP', 'Flagella', 'Leishmania donovani', 'Microscopy, Confocal', 'Microscopy, Electron, Transmission', 'Movement', 'Time-Lapse Imaging']
| 27,849,021
|
[['A11.284.430.214.190.750.602.309'], ['D03.633.100.759.646.138.395', 'D13.695.462.200', 'D13.695.667.138.395', 'D13.695.827.068.395'], ['A11.284.180.290'], ['B01.268.475.868.488.230'], ['E01.370.350.515.395', 'E05.595.395'], ['E01.370.350.515.402.580', 'E05.595.402.580'], ['G07.568', 'G11.427.410'], ['E01.370.350.600.817', 'E05.712.657', 'L01.280.960.399']]
|
['Anatomy [A]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Information Science [L]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Forgiveness: Moving on can
|
Forgiveness as a procedure has been gaining prominence. The objective of this article is to provide the basics of forgiveness as an intervention so primary care clinicians can facilitate its use. These clinicians include but are not limited to physicians, nurses, medical assistants, pharmacy professionals, physical therapists, social workers, psychologists, case managers, and clergy. This narrative explains the rationale for understanding forgiveness as a procedure and ways to explain it. To assist clinicians and patients in making informed decisions, samples of forgiveness research are included that describe its positive relationship to specific physical health situations. The article also describes an evidence-based forgiveness therapy, circumstances in which it is harmful to forgive oneself or others, describes the limitations of this article, and suggests future directions.
|
['Cognitive Behavioral Therapy', 'Female', 'Forgiveness', 'Health Status', 'Humans', 'Interpersonal Relations', 'Male', 'Mental Health', 'Primary Health Care']
| 31,735,110
|
[['F04.754.137.350'], ['F01.470.383', 'F01.829.401.166'], ['I01.240.425', 'N01.224.425', 'N06.850.505.400.425'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F01.829.401'], ['F02.418', 'N01.400.500'], ['N04.590.233.727']]
|
['Psychiatry and Psychology [F]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]', 'Organisms [B]']
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 0
|
A Comparison of the ATP Generating Pathways Used by S. Typhimurium to Fuel Replication within Human and Murine Macrophage and Epithelial Cell Lines.
|
The metabolism of S. Typhimurium within infected host cells plays a fundamental role in virulence since it enables intracellular proliferation and dissemination and affects the innate immune response. An essential requirement for the intracellular replication of S. Typhimurium is the need to regenerate ATP. The metabolic route used to fulfil this requirement is the subject of the present study. For infection models we used human and murine epithelial and macrophage cell lines. The epithelial cell lines were mICc12, a transimmortalised murine colon enterocyte cell line that shows many of the characteristics of a primary epithelial cell line, and HeLa cells. The model macrophage cell lines were THP-1A human monocyte/macrophages and RAW 264.7 murine macrophages. Using a mutational approach combined with an exometabolomic analysis, we showed that neither fermentative metabolism nor anaerobic respiration play major roles in energy generation in any of the cell lines studied. Rather, we identified overflow metabolism to acetate and lactate as the foremost route by which S. Typhimurium fulfils its energy requirements.
|
['Adenosine Triphosphate', 'Animals', 'Cell Line', 'Glycolysis', 'HeLa Cells', 'Humans', 'Intestinal Mucosa', 'Macrophages', 'Metabolic Networks and Pathways', 'Metabolomics', 'Mice', 'Salmonella typhimurium', 'Ubiquinone']
| 26,930,214
|
[['D03.633.100.759.646.138.236', 'D13.695.667.138.236', 'D13.695.827.068.236'], ['B01.050'], ['A11.251.210'], ['G02.111.158.750', 'G03.191.750', 'G03.295.436', 'G03.493.360'], ['A11.251.210.190.400', 'A11.251.860.180.400', 'A11.436.340'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A03.556.124.369', 'A10.615.550.444'], ['A11.329.372', 'A11.627.482', 'A11.733.397', 'A15.382.670.522', 'A15.382.680.397'], ['G03.493'], ['H01.158.201.586', 'H01.158.273.180.599', 'H01.181.122.638'], ['B01.050.150.900.649.313.992.635.505.500'], ['B03.440.450.425.800.200.825', 'B03.660.250.150.710.160.760'], ['D02.806.250.900', 'D08.211.935']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Disciplines and Occupations [H]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
Development of real-time RT-PCR for detecting viable Cochlodinium polykrikoides (Dinophyceae) cysts in sediment.
|
Morphological observations have confirmed that cysts are produced by dinoflagellates. However, finding a seed bed or unknown cysts in field samples by microscopy is extremely time consuming. Real-time PCR has been used to facilitate the detection of dinoflagellate cysts in sediment. However, DNA from dead vegetative cells remaining on the surface sediment may persist for a long period of time, which can cause false positive DNA detection. In this study, a non-quantitative RNA targeted probe using real-time RT-PCR was developed for detection of viable cysts in sediment. Large-subunit rRNA was used to develop a species-specific RNA targeted probe for the ichthyotoxic dinoflagellate Cochlodinium polykrikoides. The sediment samples were sieved and incubated at 30°C for 3h prior to RNA extraction to remove RNA from dead cells remaining in the sediment. Nested-PCR was conducted to maximize assay sensitivity. A field survey to determine the distribution of cysts at 155 sampling stations in the western and southern part of the Korean peninsula showed that C. polykrikoides cysts were detected at five sampling stations.
|
['DNA, Protozoan', 'Dinoflagellida', 'Environmental Monitoring', 'Harmful Algal Bloom', 'RNA, Ribosomal', 'Real-Time Polymerase Chain Reaction', 'Reverse Transcriptase Polymerase Chain Reaction', 'Seawater', 'Sensitivity and Specificity']
| 28,073,561
|
[['D13.444.308.442'], ['B01.043.214'], ['N06.850.460.350.080', 'N06.850.780.375'], ['G16.500.285.400'], ['D13.444.735.686'], ['E05.393.620.500.706'], ['E05.393.620.500.725'], ['G16.500.275.725.500'], ['E05.318.370.800', 'E05.318.740.872', 'G17.800', 'N05.715.360.325.700', 'N05.715.360.750.725', 'N06.850.520.445.800', 'N06.850.520.830.872']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Dependence on macrophages of the guinea pig T-cell immune response to Herpetomonas samuelpessoai.
|
The specific in vitro activation of guinea pig T-lymphocytes by Herpetomonas samuelpessoai and its dependence upon macrophages was studied. Peritoneal exudate T-cells from guinea pigs sensitized against intact H. samuelpessoai organisms were activated specifically in vitro as measured by 3H thymidine incorporation. These same T-cells also were activated by Trypanosoma cruzi extracts, indicating cross reactivity at the cellular level between the two protozoan antigens. The T-cell specific response was found to be dependent on adherent cells. Moreover, macrophages that had been "pulsed" in vitro with H. samuelpessoai organisms were able to induce the specific T-cell activation.
|
['Animals', 'Eukaryota', 'Guinea Pigs', 'Immunization', 'Lymphocyte Activation', 'Macrophages', 'T-Lymphocytes']
| 6,973,620
|
[['B01.050'], ['B01'], ['B01.050.150.900.649.313.992.550'], ['E02.095.465.425.400', 'E05.478.550', 'N02.421.726.758.310', 'N06.850.780.200.425', 'N06.850.780.680.310'], ['E01.370.225.812.482', 'E05.200.812.482', 'E05.478.594.530', 'G12.450.050.400.545', 'G12.565'], ['A11.329.372', 'A11.627.482', 'A11.733.397', 'A15.382.670.522', 'A15.382.680.397'], ['A11.118.637.555.567.569', 'A15.145.229.637.555.567.569', 'A15.382.490.555.567.569']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Anatomy [A]']
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Effect of Reducing Abdominal Compression during Prone CT Colonography on Ascending Colonic Rotation during Supine-to-Prone Positional Change.
|
OBJECTIVE: To determine the effect of reduced abdominal compression in prone position on ascending colonic movement during supine-to-prone positional change during CT colonography (CTC).MATERIALS AND METHODS: Eighteen consecutive patients who had undergone prone CTC scanning with cushion blocks placed under the chest and hip/thigh to reduce abdominal compression and had confirmed sessile polyps ? 6 mm in the well-distended, straight, mid-ascending colon, were included. Radial location along the ascending colonic luminal circumference (°) was measured for 24 polyps and 54 colonic teniae on supine and prone CTC images. The supine-to-prone change ranging between -180° and +180° (- and + for internal and external colonic rotations, respectively), was determined. In addition, possible causes of any ascending colonic rotations were explored.RESULTS: Abdominal compression during prone CTC scanning completely disappeared with the use of cushion blocks in 17 of 18 patients. However, some degrees of ascending colonic rotation were still observed, with the radial location changes of -22° to 61° (median, 13.9°) for the polyps and similar degrees for teniae. Fifty-four percent and 56% of polyps and teniae, respectively, showed changes > 10°. The radial location change of the polyps was significantly associated with the degree of anterior shift of the small bowel and mesentery (r = 0.722, p < 0.001) and the degree of posterior displacement of the ascending colon (r = 0.566, p = 0.004) during supine-to-prone positional change.CONCLUSION: Ascending colonic rotation upon supine-to-prone positional change during CTC, mostly in the form of external rotation, is not eliminated by removing abdominal compression in prone position.
|
['Aged', 'Colon', 'Colonic Polyps', 'Colonography, Computed Tomographic', 'Female', 'Humans', 'Male', 'Middle Aged', 'Movement', 'Prone Position', 'Retrospective Studies', 'Rotation']
| 26,798,215
|
[['M01.060.116.100'], ['A03.556.124.526.356', 'A03.556.249.249.356'], ['C23.300.825.411.235'], ['E01.370.350.350.810.180', 'E01.370.350.600.350.700.810.180', 'E01.370.350.700.700.810.180', 'E01.370.350.700.810.810.180', 'E01.370.372.230'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['G07.568', 'G11.427.410'], ['G11.427.695.525'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['G01.482.703']]
|
['Named Groups [M]', 'Anatomy [A]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Health Care [N]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Sequential designs with small samples: Evaluation and recommendations for normal responses.
|
Sequential monitoring is a well-known methodology for the design and analysis of clinical trials. Driven by the lower expected sample size, recent guidelines and published research suggest the use of sequential methods for the conduct of clinical trials in rare diseases. However, the vast majority of the developed and most commonly used sequential methods relies on asymptotic assumptions concerning the distribution of the test statistics. It is not uncommon for trials in (very) rare diseases to be conducted with only a few decades of patients and the use of sequential methods that rely on large-sample approximations could inflate the type I error probability. Additionally, the setting of a rare disease could make the traditional paradigm of designing a clinical trial (deciding on the sample size given type I and II errors and anticipated effect size) irrelevant. One could think of the situation where the number of patients available has a maximum and this should be utilized in the most efficient way. In this work, we evaluate the operational characteristics of sequential designs in the setting of very small to moderate sample sizes with normally distributed outcomes and demonstrate the necessity of simple corrections of the critical boundaries. We also suggest a method for deciding on an optimal sequential design given a maximum sample size and some (data driven or based on expert opinion) prior belief on the treatment effect.
|
['Clinical Trials as Topic', 'Humans', 'Rare Diseases', 'Research Design', 'Sample Size']
| 27,342,574
|
[['E05.318.372.250.250', 'N05.715.360.330.250.250', 'N06.850.520.450.250.250'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C23.550.291.906'], ['E05.581.500', 'H01.770.644.728'], ['E05.318.370.762', 'E05.581.500.902', 'N05.715.360.325.692', 'N06.850.520.445.762']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Diseases [C]', 'Disciplines and Occupations [H]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
|
Attenuation of the pressor activity of endogenous vasoconstrictors by sodium nitroprusside in the pithed rat.
|
The present study characterized the effects of sodium nitroprusside (SNP) on the responsiveness of pithed rats to pressor stimuli. Intravenous infusions of SNP produced a dose-related inhibition of the pressor responses to stimulation of sympathetic preganglionic neurons in the thoracolumbar region of the spinal cord. The site at which SNP affected the vasoconstrictor response appeared to be post-junctional because norepinephrine responses were also attenuated, as were blood pressure increases to angiotensin II and vasopressin. Responsiveness to all of the pressor stimuli was restored when the infusion of SNP was terminated. The effects of SNP could not be attributed simply to observed reductions in baseline blood pressure because pretreatment of the pithed rats with captopril, which produced an initial hypotensive effect equivalent to that seen after SNP, did not alter the pressor responses to angiotensin II or vasopressin. However, like SNP, captopril did attenuate the blood pressure increments to spinal cord stimulation and norepinephrine. These results suggest that a part of the hypotensive potency of SNP may result from its ability to antagonize vasoconstriction mediated by norepinephrine, angiotensin II and vasopressin.
|
['Angiotensin II', 'Animals', 'Blood Pressure', 'Captopril', 'Dose-Response Relationship, Drug', 'Electric Stimulation', 'Ferricyanides', 'Heart Rate', 'Infusions, Intravenous', 'Male', 'Nitroprusside', 'Norepinephrine', 'Pressoreceptors', 'Rats', 'Rats, Inbred Strains', 'Spinal Cord', 'Vasopressins']
| 2,663,242
|
[['D06.472.699.094.078', 'D12.644.400.070.078', 'D12.644.456.073.041', 'D12.644.548.058.078', 'D12.776.631.650.070.078', 'D23.469.050.050.050'], ['B01.050'], ['E01.370.600.875.249', 'G09.330.380.076'], ['D12.125.072.401.623.270'], ['G07.690.773.875', 'G07.690.936.500'], ['E05.723.402'], ['D01.248.497.158.291.350', 'D01.490.100.300', 'D01.625.400.100.325'], ['E01.370.600.875.500', 'G09.330.380.500'], ['E02.319.267.082.500', 'E02.319.267.510.590'], ['D01.248.497.158.291.350.550', 'D01.490.100.300.550', 'D01.625.400.100.325.550'], ['D02.033.100.291.502', 'D02.092.063.480', 'D02.092.211.215.746', 'D02.092.311.830', 'D02.455.426.559.389.657.166.175.830'], ['A08.675.650.915.750.750', 'A08.800.050.800.900.700', 'A08.800.950.750.750', 'A11.671.650.915.750.750'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.050.199.520.760', 'B01.050.150.900.649.313.992.635.505.700.400'], ['A08.186.854'], ['D06.472.699.631.692.781', 'D12.644.400.900', 'D12.644.456.925', 'D12.644.548.691.692.781', 'D12.776.631.650.937']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[An unusual pneumomediastinum case in a child caused by spontaneous bronchial rupture].
|
We report a case of spontaneous pneumomediastinum (SPM) in a 3 year-old child, admitted to the emergency department because he presented dyspnea for a few hours, after a paroxysm of cough. The SPM is rare in children; the term "spontaneous" is reserved for cases of pneumomediastinum that haven't a traumatic cause. SPM is seen most commonly in asthmatics and in any patient who induces a Valsalva maneuver. The clinical diagnosis is confirmed by chest radiograph. When the diagnosis is uncertain, the chest CT scan is considered the gold standard of imaging tests, capable of detecting pneumomediastinum even in patients with small amounts of mediastinal air. In this case CT images showed the cause: spontaneous bronchial rupture. The direct sign of bronchial injury is the contiguity of the luminal air with that in the mediastinum. In the literature SPM cases are very rare, at least in health patients without tracheobronchial anomalies. The SPM is generally a benign entity that requires supportive care, and resolution occurs spontaneously, such as in our patient. In this article we want to explain the main clinical, diagnostic and therapeutic aspects of SPM, because, even if it's rare in children, it must be considered in the differential diagnosis of dyspnea; then we want to demonstrate as, in this case, a TC scan was important to identifying the SPM cause: a bronchial rupture.
|
['Anti-Bacterial Agents', 'Bronchi', 'Bronchodilator Agents', 'Child, Preschool', 'Diagnosis, Differential', 'Drug Therapy, Combination', 'Dyspnea', 'Female', 'Glucocorticoids', 'Humans', 'Mediastinal Emphysema', 'Oxygen Inhalation Therapy', 'Radiography, Thoracic', 'Rupture, Spontaneous', 'Tomography, X-Ray Computed', 'Treatment Outcome']
| 22,495,198
|
[['D27.505.954.122.085'], ['A04.411.125'], ['D27.505.696.663.050.110', 'D27.505.954.796.050.100'], ['M01.060.406.448'], ['E01.171'], ['E02.319.310'], ['C08.618.326', 'C23.888.852.371'], ['D06.472.040.543', 'D27.505.696.399.472.488'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C08.846.187.290', 'C23.550.325.250'], ['E02.880.690'], ['E01.370.350.700.730'], ['C23.300.909'], ['E01.370.350.350.810', 'E01.370.350.600.350.700.810', 'E01.370.350.700.700.810', 'E01.370.350.700.810.810', 'E01.370.350.825.810.810'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Chemicals and Drugs [D]', 'Anatomy [A]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Organisms [B]', 'Health Care [N]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.