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Functional properties of partially oxidized trout hemoglobins.
|
This paper reports on a study of the effect of partial oxidation on oxygen and carbon monoxide binding by components I and IV of trout hemoglobin. The O2 binding equilibria of the various oxidation mixtures show a decrease in the heme-heme interactions as the number of oxidized sites is increased. However, the large Bohr effect, characteristic of Hb Trout IV, is maintained unchanged. Similarly the time course of CO combination changes on increasing the fractional oxidation, and the autocatalytic character of the CO binding kinetics is lost; however the pH dependence of the apparent "on" constant in the oxidation mixtures is similar to that characteristic of the native molecule. The results of the O2 equilibria and of CO binding kinetics may be interpreted in accordance with the two state concerted model suggesting that in the oxidation intermediates there is an increase in the fraction of the high affinity (R) conformation. Additional experiments on the effect of azide, and fluoride, ferric ligands which produce a change of spin state of the heme iron, suggest that additional second order conformational changes may also come into play.
|
['Animals', 'Azides', 'Carbon Monoxide', 'Carboxyhemoglobin', 'Hemoglobins', 'Hydrogen-Ion Concentration', 'Kinetics', 'Oxidation-Reduction', 'Oxygen', 'Oxyhemoglobins', 'Protein Conformation', 'Salmonidae', 'Structure-Activity Relationship', 'Trout']
| 13,851
|
[['B01.050'], ['D01.625.100', 'D02.159'], ['D01.200.250', 'D01.362.200', 'D01.650.550.250'], ['D12.776.124.400.141', 'D12.776.422.316.762.149'], ['D12.776.124.400', 'D12.776.422.316.762'], ['G02.300'], ['G01.374.661', 'G02.111.490'], ['G02.700', 'G03.295.531'], ['D01.268.185.550', 'D01.362.670'], ['D12.776.124.400.707', 'D12.776.422.316.762.687'], ['G02.111.570.820.709'], ['B01.050.150.900.493.817.750'], ['G02.111.830', 'G07.690.773.997'], ['B01.050.150.900.493.817.750.825']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
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|
Inadequate glucose control in type 2 diabetes is associated with impaired lung function and systemic inflammation: a cross-sectional study.
|
BACKGROUND: Inadequate glucose control may be simultaneously associated with inflammation and decreased lung function in type 2 diabetes. We evaluated if lung function is worse in patients with inadequate glucose control, and if inflammatory markers are simultaneously increased in these subjects.METHODS: Subjects were selected at the Colombian Diabetes Association Center in Bogot?. Pulmonary function tests were performed and mean residual values were obtained for forced expiratory volume (FEV1), forced vital capacity (FVC) and FEV1/FVC, with predicted values based on those derived by Hankinson et al. for Mexican-Americans. Multiple least-squares regression was used to adjust for differences in known determinants of lung function. We measured blood levels of glycosylated hemoglobin (HBA1c), interleukin 6 (IL-6), tumor necrosis factor (TNF-alpha), fibrinogen, ferritin, and C-reactive protein (C-RP).RESULTS: 495 diabetic patients were studied, out of which 352 had inadequate control (HBA1c > 7%). After adjusting for known determinants of lung function, those with inadequate control had lower FEV1 (-75.4 mL, IC95%: -92, -59; P < 0.0001) and FVC (-121 mL, IC95%: -134, -108; P < 0,0001) mean residuals, and higher FEV1/FVC (0.013%, IC95%: 0.009, 0.018, P < 0.0001) residuals than those with adequate control, as well as increased levels of all inflammatory markers (P < 0.05), with the exception of IL-6.CONCLUSIONS: Subjects with type 2 diabetes and inadequate control had lower FVC and FEV1 than predicted and than those of subjects with adequate control. It is postulated that poorer pulmonary function may be associated with increased levels of inflammatory mediators.
|
['Adult', 'Aged', 'Biomarkers', 'Blood Glucose', 'C-Reactive Protein', 'Cross-Sectional Studies', 'Diabetes Mellitus, Type 2', 'Female', 'Ferritins', 'Fibrinogen', 'Glycated Hemoglobin A', 'Humans', 'Hyperglycemia', 'Inflammation', 'Interleukin-6', 'Lung Diseases', 'Male', 'Middle Aged', 'Respiratory Function Tests', 'Tumor Necrosis Factor-alpha']
| 20,659,337
|
[['M01.060.116'], ['M01.060.116.100'], ['D23.101'], ['D09.947.875.359.448.500'], ['D12.776.034.145', 'D12.776.124.050.120', 'D12.776.124.486.157'], ['E05.318.372.500.875', 'N05.715.360.330.500.875', 'N06.850.520.450.500.875'], ['C18.452.394.750.149', 'C19.246.300'], ['D12.776.157.427.249', 'D12.776.556.579.249'], ['D12.776.124.050.250', 'D12.776.124.125.500', 'D12.776.811.300', 'D23.119.490'], ['D09.400.430.937', 'D12.776.124.400.405.440', 'D12.776.395.381', 'D12.776.422.316.762.380.440'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C18.452.394.952'], ['C23.550.470'], ['D12.644.276.374.465.224', 'D12.776.467.374.465.202', 'D23.529.374.465.224'], ['C08.381'], ['M01.060.116.630'], ['E01.370.386.700'], ['D12.644.276.374.500.800', 'D12.644.276.374.750.626', 'D12.776.124.900', 'D12.776.395.930', 'D12.776.467.374.500.800', 'D12.776.467.374.750.626', 'D23.529.374.500.800', 'D23.529.374.750.626']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Diseases [C]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
The contribution of handsearching European general health care journals to the Cochrane Controlled Trials Register.
|
A fundamental aim of any systematic review is that all relevant studies should be identified and considered for inclusion. Limitations with searching bibliographic databases led the Cochrane Collaboration to search journals by hand for reports of trials. This article presents the results of a 3-year project to identify and make accessible reports of randomized trials published in European general health care journals. Overall, 21,620 reports of controlled trials were identified from 119 journals from 16 countries. More than three quarters (76%) were published in U.K. or German journals. Only 3,640 (17%) reports were indexed in MEDLINE as controlled trials, and 6,554 (30%) were not indexed in MEDLINE at all. Bibliographic details for all reports are available by searching The Cochrane Controlled Trials Register in The Cochrane Library. This project has ensured that a large proportion of trial reports not previously identifiable has been made accessible to those preparing systematic reviews.
|
['Abstracting and Indexing', 'Databases, Bibliographic', 'Europe', 'Evidence-Based Medicine', 'Humans', 'Information Services', 'Information Storage and Retrieval', 'International Cooperation', 'MEDLINE', 'Organizations, Nonprofit', 'Periodicals as Topic', 'Randomized Controlled Trials as Topic']
| 11,868,446
|
[['L01.453.245.100'], ['L01.313.500.750.300.188.300', 'L01.470.750.500'], ['Z01.542'], ['H02.249.750', 'H02.403.200.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['L01.453'], ['L01.313.500.750.280', 'L01.470'], ['I01.615.500'], ['L01.313.500.750.280.710.500', 'L01.313.500.750.280.750.500', 'L01.313.500.750.300.188.300.650.500', 'L01.313.500.750.300.710.500', 'L01.313.500.750.300.742.650.500', 'L01.470.750.500.650.500'], ['N03.540.630'], ['L01.178.682.829.678'], ['E05.318.372.250.250.365.500', 'N05.715.360.330.250.250.365.500', 'N06.850.520.450.250.250.365.500']]
|
['Information Science [L]', 'Geographicals [Z]', 'Disciplines and Occupations [H]', 'Organisms [B]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 1
|
Lower Frequency of HLA-DRB1*01 in Southwestern Iranian Patients with Atherosclerosis.
|
BACKGROUND: Human leukocyte antigen (HLA) complex is a gene family involved in antigen presentation associated with protection or susceptibility to inflammatory, infectious and autoimmune diseases. Atherosclerosis is a chronic inflammatory disease in which HLA molecules play a role in the initiation and development of the disease through presentation of self or foreign antigens to T cells.OBJECTIVE: To investigate the association of HLA-DRB1 alleles with atherosclerosis in a sample of southwestern Iranians.METHODS: We performed an analytical cross-sectional study involving 96 patients with atherosclerosis and 72 controls. HLA-DRB1 genotyping was performed by PCR-SSP method.RESULTS: We observed a significantly lower frequency of DRB1*01 in patients with coronary artery atherosclerosis than in controls (4.68% vs. 13.1, P=0.0052, OR=3.09, CI 95%: 1.35-7.05). However, this allele showed a positive association with high blood pressure (P=0.009) in patients. Furthermore, DRB1*16 allele was associated with hyperlipidemia (P=0.008) in patients.CONCLUSION: Our results demonstrated that DRB1*01 may be a protective allele against atherosclerosis in individuals who live in southwest of Iran. The mechanism of this protection needs further investigation.
|
['Adult', 'Aged', 'Aged, 80 and over', 'Antigen Presentation', 'Atherosclerosis', 'Cross-Sectional Studies', 'Female', 'Gene Frequency', 'Genetic Association Studies', 'Genotype', 'HLA-DRB1 Chains', 'Humans', 'Hypertension', 'Iran', 'Male', 'Middle Aged', 'Polymorphism, Genetic']
| 30,246,695
|
[['M01.060.116'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['G12.119', 'G12.450.050.400.070'], ['C14.907.137.126.307'], ['E05.318.372.500.875', 'N05.715.360.330.500.875', 'N06.850.520.450.500.875'], ['G05.330'], ['E05.393.385'], ['G05.380'], ['D12.776.395.550.509.400.440.200.010', 'D12.776.543.550.440.400.440.200.010', 'D23.050.301.500.400.400.440.200.010', 'D23.050.301.500.450.400.440.333.500', 'D23.050.705.552.410.400.440.200.010', 'D23.050.705.552.450.400.440.333.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C14.907.489'], ['Z01.252.245.500.350'], ['M01.060.116.630'], ['G05.365.795']]
|
['Named Groups [M]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Geographicals [Z]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Missed pulmonary embolism on abdominal CT.
|
OBJECTIVE: The purpose of this study is to evaluate a series of missed pulmonary emboli (PE) identified on abdominal CT and to describe their characteristics and the clinical scenario.MATERIALS AND METHODS: All reports of chest CT scans performed during a 12-month period were searched for keywords indicative of PE. Among patients with PE, patients who also underwent an enhanced abdominal CT within 3 months were assessed for missed PE. Three radiologists reviewed the abdominal CT to confirm the presence of a missed PE. Missed PEs were classified as unknown or known. Each study was assessed for characteristics of the missed PE and the image quality of the PE study. The electronic medical record was used to document the clinical context in which the PE occurred.RESULTS: Eighteen patients (12 men and six women; average age, 58.8 years) were identified as having missed PE on abdominal CT. In seven patients (38.9%), the PE had not been previously diagnosed. Most of the missed PEs were segmental, but three missed PEs occurred in lobar vessels. In a slight majority of the cases, the reviewing radiologists judged the contrast bolus as good. The abdominal CT on which PE was overlooked was obtained for a variety of reasons, most commonly because of abdominal pain or to follow up a preexisting condition.CONCLUSION: This study shows that missed PE can occur on abdominal CT. It is recommended that interpretation include a careful search of the lower pulmonary arterial vasculature on contrast-enhanced abdominal CT scans.
|
['Adult', 'Aged', 'Aged, 80 and over', 'Contrast Media', 'Diagnostic Errors', 'Female', 'Humans', 'Male', 'Middle Aged', 'Pulmonary Embolism', 'Tomography, X-Ray Computed']
| 24,660,700
|
[['M01.060.116'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['D27.505.259.500', 'D27.720.259'], ['E01.354', 'N02.421.450.280'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['C08.381.746', 'C14.907.355.350.700'], ['E01.370.350.350.810', 'E01.370.350.600.350.700.810', 'E01.370.350.700.700.810', 'E01.370.350.700.810.810', 'E01.370.350.825.810.810']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Quality of bevacizumab (Avastin®) repacked in single-use glass vials for intravitreal administration.
|
Purpose:: Avastin® (bevacizumab) is an anti-vascular endothelial growth factor (VEGF) monoclonal antibody given as an off-label drug by intravitreal administration for treatment of ocular diseases. The drug's clinical application and its cost-benefit profile has generated demand for its division into single-use vials to meet the low volume and low-cost doses necessary for intraocular administration. However, the safety of compounding the drug in single-use vials is still under discussion. In this study, the stability and efficacy of Avastin® repacked in individual single-use glass vials and glass ampoules by external compounding pharmacies were evaluated.Methods:: Polyacrylamide gel electrophoresis (PAGE), size-exclusion chromatography (SEC), dynamic light scattering (DLS), and turbidimetry were selected to detect the formation of aggregates of various sizes. Changes in bevacizumab biological efficacy were investigated by using an enzyme-linked immunosorbent assay (ELISA).Results:: Repacked and reference bevacizumab showed similar results when analyzed by PAGE. By SEC, a slight increase in high molecular weight aggregates and a reduction in bevacizumab monomers were observed in the products of the three compounding pharmacies relative to those in the reference bevacizumab. A comparison of repacked and reference SEC chromatograms showed that the mean monomer loss was ?1% for all compounding pharmacies. Protein aggregates in the nanometer- and micrometer-size ranges were not detected by DLS and turbidimetry. In the efficacy assay, the biological function of repacked bevacizumab was preserved, with <3% loss of VEGF binding capacity relative to that of the reference.Conclusion:: The results showed that bevacizumab remained stable after compounding in ampoules and single-use glass vials; no significant aggregation, fragmentation, or loss of biological activity was observed.
|
['Angiogenesis Inhibitors', 'Bevacizumab', 'Chromatography, Gel', 'Drug Packaging', 'Drug Stability', 'Dynamic Light Scattering', 'Electrophoresis, Polyacrylamide Gel', 'Enzyme-Linked Immunosorbent Assay', 'Intravitreal Injections', 'Molecular Weight', 'Nephelometry and Turbidimetry', 'Quality Control', 'Vascular Endothelial Growth Factor A']
| 28,591,284
|
[['D27.505.696.377.077.099', 'D27.505.696.377.450.100', 'D27.505.954.248.025'], ['D12.776.124.486.485.114.224.060.375', 'D12.776.124.790.651.114.224.060.438', 'D12.776.377.715.548.114.224.200.438'], ['E05.196.181.400.250'], ['E05.916.320', 'J01.576.655.750.321', 'J01.576.761.300'], ['E05.916.330'], ['E05.196.822.325'], ['E05.196.401.402', 'E05.301.300.319'], ['E05.478.566.350.170', 'E05.478.566.380.360', 'E05.478.583.400.170', 'E05.601.470.350.170', 'E05.601.470.380.360'], ['E02.319.267.530.475.500'], ['G02.494'], ['E05.196.712.650'], ['J01.897.608'], ['D12.644.276.100.800.200', 'D12.776.467.100.800.200', 'D23.529.100.800.200']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Technology, Industry, and Agriculture [J]', 'Phenomena and Processes [G]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Echo detection and target-ranging neurons in the auditory system of the bat Eptesicus fuscus.
|
Some of the neurons in the nucleus intercollicularis and auditory cortex of the echolocating bat Eptesicus fuscus respond selectively to sonar echoes occurring with specific echo delays or pulse-echo intervals. They do not respond for a wide range of other types of sounds or for sonar echoes at longer or shorter pulse-echo intervals; they may, therefore, be specialized for detection and ranging of sonar targets.
|
['Animals', 'Auditory Cortex', 'Auditory Perception', 'Chiroptera', 'Echolocation', 'Inferior Colliculi', 'Neurons', 'Orientation']
| 705,350
|
[['B01.050'], ['A08.186.211.200.885.287.500.814.249', 'A08.186.211.200.885.287.500.863.297'], ['F02.463.593.071', 'G07.888.125'], ['B01.050.150.900.649.313.937'], ['F01.145.113.055.400'], ['A08.186.211.132.659.800.407'], ['A08.675', 'A11.671'], ['F01.058.577', 'F02.830.606']]
|
['Organisms [B]', 'Anatomy [A]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
A study of goat SRY protein expression suggests putative new roles for this gene in the developing testis of a species with long-lasting SRY expression.
|
The testis-determining gene SRY is not well-conserved among mammals, and particularly between mouse and other mammals. To evaluate SRY function in a nonrodent species, we produced an antibody against goat SRY and used it to investigate the expression pattern of SRY throughout goat testicular development. By contrast with the mouse, SRY is primarily expressed in most cells of XY genital-ridges and not solely in pre-Sertoli cells. Between cord formation and prepuberty, SRY remains expressed in both Sertoli and germinal cells. During adulthood, SRY expression declines and then disappears from meiotic germ cells, only remaining present at low levels in some spermatogonia. Unlike the germinal lineage, SRY continues to be highly expressed in adult Sertoli cells with a typical nuclear staining. Our data indicate that in goat, the role of SRY may not be limited to testis determination and could have other functions in testicular maintenance and hence male fertility.
|
['Animals', 'Blotting, Western', 'COS Cells', 'Chlorocebus aethiops', 'Female', 'Gene Expression Regulation, Developmental', 'Goats', 'Male', 'Reverse Transcriptase Polymerase Chain Reaction', 'Sertoli Cells', 'Sex Differentiation', 'Sex-Determining Region Y Protein', 'Spermatogonia', 'Testis']
| 20,941,779
|
[['B01.050'], ['E05.196.401.143', 'E05.301.300.096', 'E05.478.566.320.200', 'E05.601.262', 'E05.601.470.320.200'], ['A11.251.210.172.500', 'A11.329.228.220'], ['B01.050.150.900.649.313.988.400.112.199.120.126.110'], ['G05.308.310'], ['B01.050.150.900.649.313.500.380.513'], ['E05.393.620.500.725'], ['A05.360.444.849.789', 'A11.382.952', 'A11.436.837'], ['G07.345.500.325.377.843', 'G07.345.750.500', 'G08.686.841.500'], ['D12.776.260.719.049', 'D12.776.660.235.400.750.049', 'D12.776.664.235.400.750.049', 'D12.776.930.823.049'], ['A05.360.490.890.900', 'A11.497.760.800'], ['A05.360.444.849', 'A05.360.576.782', 'A06.300.312.782']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Serum protein analysis and bone marrow cytology in patients with an extreme elevation of the erythrocyte sedimentation rate.
|
One hundred patients in whom an Erythrocyte Sedimentation Rate (ESR) in excess of 100 mm. in the first hour was found on 2 consecutive occasions were investigated. Serum protein electrophoresis was performed on 96 of these patients and bone marrow examination on 55 patients. Acute infection was the commonest diagnosis though the majority of patients had 2 or more separate conditions each contributing to the elevation of the ESR. Quantitive serum protein electrophoresis was abnormal in all but one patient and was of limited diagnostic value. A definite band in the globulin region was detected in 11 patients, 7 of whom were found to have myelomatosis. Bone marrow examination was useful only in patients with a discrete band in the globulin fraction or with a specific haematological abnormality. It is suggested, therefore, that bone marrow examination be confined to patients with such abnormalities irrespective of an elevation of their ESR.
|
['Adult', 'Aged', 'Blood Protein Electrophoresis', 'Blood Proteins', 'Blood Sedimentation', 'Bone Marrow', 'Bone Marrow Examination', 'Female', 'Humans', 'Male', 'Middle Aged']
| 644,295
|
[['M01.060.116'], ['M01.060.116.100'], ['E01.370.225.124.100.110', 'E05.196.401.076', 'E05.200.124.100.110', 'E05.301.300.049'], ['D12.776.124'], ['E01.370.225.625.125', 'E05.200.625.125'], ['A15.382.216'], ['E01.370.225.625.135', 'E05.200.625.135'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Organisms [B]']
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Kinetics of CO binding to and dissociation from microsomal cytochrome P-450 induced by phenobarbital in rat liver.
|
The kinetics of CO binding to hepatic microsomes from phenobarbital-treated Sprague-Dawley rats, measured by stopped flow spectrophotometry, can be resolved into three components with second order velocity constants of 2.23 +/- 0.35 X 10(5) M-1 S-1, 1.59 +/- 0.18 X 10(6) M-1 S-1, and 8.7 +/- 1.7 X 10(6) M-1 S-1. The three CO-binding species were present in ratios of 1:1.25:1.39 as judged by the relative amplitude of the change in absorbance at 450 nm associated with each of the kinetic components. Similar results were obtained in a range of [CO] from 10 to 700 micron when CO recombination was followed subsequent to flash photolysis of the CO-associated microsomes. In contrast, the dissociation rate of CO from its cytochrome P-450 complex measured by the NO replacement method was biphasic. Approximately 40% of the bound CO dissociated at a rate of 0.40 +/- 0.071 s-1, whereas the remaining 60% dissociated at a rate of 0.049 +/- 0.008 s-1 at 20 degrees C.
|
['Animals', 'Carbon Monoxide', 'Cytochrome P-450 Enzyme System', 'Kinetics', 'Male', 'Mathematics', 'Microsomes, Liver', 'Phenobarbital', 'Protein Binding', 'Rats']
| 659,421
|
[['B01.050'], ['D01.200.250', 'D01.362.200', 'D01.650.550.250'], ['D08.244.453', 'D08.811.682.690.708.170', 'D12.776.422.220.453'], ['G01.374.661', 'G02.111.490'], ['H01.548'], ['A11.284.835.540.541'], ['D03.383.742.698.253.650'], ['G02.111.679', 'G03.808'], ['B01.050.150.900.649.313.992.635.505.700']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Disciplines and Occupations [H]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
Complicated duodenal ulcer, vagotomy or distal resection--own experience.
|
An analysis of clinical material through 10-year-period (1980-1989) is presented. There were 708 gastric operations performed for gastroduodenal ulcer disease. Most frequently distal gastric resections (585) were used, 233 in B. I. and 352 in B II. modification. Selective proximal vagotomy (SPV) was less frequently used (only 116 cases). Authors think there is no reason for a general use of SPV for each form of duodenal ulcer because of very serious competition with the H2 blockers. A different individual proved choice of the method as well as an appropriate subtle operating technique is considered to be essential.
|
['Duodenal Ulcer', 'Humans', 'Methods', 'Vagotomy, Proximal Gastric']
| 1,411,241
|
[['C06.405.469.275.800.348', 'C06.405.748.586.349'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.581'], ['E04.525.210.105.600.850.850']]
|
['Diseases [C]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Effect of recombinant surfactant protein C-based surfactant on the acute respiratory distress syndrome.
|
BACKGROUND: Preclinical studies suggest that exogenous surfactant may be of value in the treatment of the acute respiratory distress syndrome (ARDS), and two phase 2 clinical trials have shown a trend toward benefit. We conducted two phase 3 studies of a protein-containing surfactant in adults with ARDS.METHODS: In two multicenter, randomized, double-blind trials involving 448 patients with ARDS from various causes, we compared standard therapy alone with standard therapy plus up to four intratracheal doses of a recombinant surfactant protein C-based surfactant given within a period of 24 hours.RESULTS: The overall survival rate was 66 percent 28 days after treatment, and the median number of ventilator-free days was 0 (68 percent range, 0 to 26); there was no significant difference between the groups in terms of mortality or the need for mechanical ventilation. Patients receiving surfactant had a significantly greater improvement in blood oxygenation during the initial 24 hours of treatment than patients receiving standard therapy, according to both univariate and multivariate analyses.CONCLUSIONS: The use of exogenous surfactant in a heterogeneous population of patients with ARDS did not improve survival. Patients who received surfactant had a greater improvement in gas exchange during the 24-hour treatment period than patients who received standard therapy alone, suggesting the potential benefit of a longer treatment course.
|
['APACHE', 'Analysis of Variance', 'Causality', 'Double-Blind Method', 'Female', 'Humans', 'Logistic Models', 'Male', 'Middle Aged', 'Oxygen', 'Pulmonary Surfactant-Associated Protein C', 'Pulmonary Surfactants', 'Recombinant Proteins', 'Respiration, Artificial', 'Respiratory Distress Syndrome', 'Survival Rate', 'Treatment Failure']
| 15,329,426
|
[['E05.318.308.980.438.475.365', 'E05.318.308.980.438.475.456.500.250', 'N05.715.360.300.800.438.375.364.500.250', 'N06.850.520.308.980.438.475.364.500.250'], ['E05.318.740.150', 'N05.715.360.750.125', 'N06.850.520.830.150'], ['N05.715.350.200', 'N06.850.490.625'], ['E05.318.370.300', 'E05.581.500.300', 'N05.715.360.325.320', 'N06.850.520.445.300'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.740.500.525', 'E05.318.740.600.800.450', 'E05.318.740.750.450', 'E05.599.835.875', 'N05.715.360.750.530.480', 'N05.715.360.750.625.700.450', 'N05.715.360.750.695.470', 'N06.850.520.830.500.525', 'N06.850.520.830.600.800.450', 'N06.850.520.830.750.450'], ['M01.060.116.630'], ['D01.268.185.550', 'D01.362.670'], ['D12.776.543.717', 'D12.776.816.750', 'D12.776.823.186'], ['D27.505.954.796.600'], ['D12.776.828'], ['E02.041.625', 'E02.365.647.729', 'E02.880.820'], ['C08.381.840', 'C08.618.840'], ['E05.318.308.985.550.900', 'N01.224.935.698.826', 'N06.850.505.400.975.550.900', 'N06.850.520.308.985.550.900'], ['E01.789.800.760', 'N04.761.559.590.800.760', 'N05.715.360.575.575.800.760']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Named Groups [M]', 'Chemicals and Drugs [D]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Immunohistological studies on an Onchocerca volvulus ankyrin (EI).
|
The distribution of an Onchocerca volvulus ankyrin, designated E1, was studied in different O. volvulus stages and other helminths by immunohistochemistry using rabbit antibodies raised against the recombinant E1 protein. In adult O. volvulus the protein designated E1 was localized to the extracellular clefts as well as to the cytoplasm adjacent to the cell membrane in the area of the basal labyrinth in hypodermis, intestine and uterus and to a lesser extent in oviduct and vas deferens. Neuronal cell bodies were also labelled. No labelling of the basal laminae, muscles or epithelia of ovary or testis was observed. Detection of the E1 protein was associated with embryonic development. Germ cells and early morulae showed no reaction; labelling was first seen in late morulae, corresponding to the stage of gastrulation, and increased in the following embryonic stages. In microfilariae the nerve ring and the cephalic space, which represents the anterior nerve-enriched portion of the body, were labelled. In third-stage larvae of O. volvulus labelling was associated with the hypodermis, and in those of Anisakis sp. the cytoplasm adjacent to the membrane of the excretory gland cell and the basal labyrinth of the hypodermis were labelled. Following anthelminthic treatment a disruption of the labelling pattern of the E1 protein was observed in adult O. volvulus with leakage of the protein into neighbouring areas. Damage to the worm was associated with reduction and finally loss of E1 protein labelling. No E1 protein was detected in dead adult worms, embryos or microfilariae. Labelling of the same organs was observed in 8 other Onchocerca species and in several other nematodes, but no reaction was seen in trematodes. The results indicate that the EI protein is associated with neuronal structures of O. volvulus, that its presence is developmentally regulated and that it has cross-reactive homologues in other nematodes. The results suggest that E1 is a functional protein. It may be useful for the assessment of parasite damage and death as well as in the characterization of the filarial nervous system.
|
['Animals', 'Anthelmintics', 'Antibodies, Helminth', 'Female', 'Humans', 'Ivermectin', 'Male', 'Onchocerca volvulus', 'Onchocerciasis']
| 8,911,440
|
[['B01.050'], ['D27.505.954.122.250.075'], ['D12.776.124.486.485.114.185', 'D12.776.124.790.651.114.185', 'D12.776.377.715.548.114.185'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D02.540.576.500.997'], ['B01.050.500.500.294.400.937.463.510.850'], ['C01.610.335.508.700.750.361.699', 'C01.610.858.650', 'C17.800.838.775.690']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Identification of sex hormone-binding globulin in the human hypothalamus.
|
Gonadal steroids are known to influence hypothalamic functions through both genomic and non-genomic pathways. Sex hormone-binding globulin (SHBG) may act by a non-genomic mechanism independent of classical steroid receptors. Here we describe the immunocytochemical mapping of SHBG-containing neurons and nerve fibers in the human hypothalamus and infundibulum. Mass spectrometry and Western blot analysis were also used to characterize the biochemical characteristics of SHBG in the hypothalamus and cerebrospinal fluid (CSF) of humans. SHBG-immunoreactive neurons were observed in the supraoptic nucleus, the suprachiasmatic nucleus, the bed nucleus of the stria terminalis, paraventricular nucleus, arcuate nucleus, the perifornical region and the medial preoptic area in human brains. There were SHBG-immunoreactive axons in the median eminence and the infundibulum. A partial colocalization with oxytocin could be observed in the posterior pituitary lobe in consecutive semithin sections. We also found strong immunoreactivity for SHBG in epithelial cells of the choroid plexus and in a portion of the ependymal cells lining the third ventricle. Mass spectrometry showed that affinity-purified SHBG from the hypothalamus and choroid plexus is structurally similar to the SHBG identified in the CSF. The multiple localizations of SHBG suggest neurohypophyseal and neuroendocrine functions. The biochemical data suggest that CSF SHBG is of brain rather than blood origin.
|
['Aged', 'Aged, 80 and over', 'Blotting, Western', 'Female', 'Humans', 'Hypothalamus', 'Immunohistochemistry', 'Male', 'Neurons', 'Sex Hormone-Binding Globulin', 'Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization']
| 16,155,373
|
[['M01.060.116.100'], ['M01.060.116.100.080'], ['E05.196.401.143', 'E05.301.300.096', 'E05.478.566.320.200', 'E05.601.262', 'E05.601.470.320.200'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A08.186.211.180.497', 'A08.186.211.200.317.357'], ['E01.370.225.500.607.512', 'E01.370.225.750.551.512', 'E05.200.500.607.512', 'E05.200.750.551.512', 'E05.478.583', 'H01.158.100.656.234.512', 'H01.158.201.344.512', 'H01.158.201.486.512', 'H01.181.122.573.512', 'H01.181.122.605.512'], ['A08.675', 'A11.671'], ['D12.776.124.790.223.800', 'D12.776.157.762', 'D12.776.377.715.182.800'], ['E05.196.566.755']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Anatomy [A]', 'Disciplines and Occupations [H]', 'Chemicals and Drugs [D]']
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
|
DICER1 Mutations and Differentiated Thyroid Carcinoma: Evidence of a Direct Association.
|
CONTEXT: DICER1 germline mutation carriers have an increased predisposition to cancer, such as pleuropulmonary blastoma (PPB) and Sertoli-Leydig cell tumor (SLCT), and a high prevalence of multinodular goiter (MNG). Although differentiated thyroid carcinoma (DTC) has been reported in some DICER1 mutation carriers with PPB treated with chemotherapy, the association of DTC with DICER1 mutations is not well established.CASE DESCRIPTION: We report a family with DICER1 mutation and familial DTC without a history of chemotherapy. A 12-year-old female (patient A) and her 14-year-old sister (patient B) presented with MNG. Family history was notable for a maternal history of DTC and bilateral ovarian SLCT. Both sisters underwent total thyroidectomy. Pathological examination showed nodular hyperplasia and focal papillary thyroid carcinoma within hyperplastic nodules. Subsequently, patient A developed virilization secondary to a unilateral ovarian SLCT. During her evaluation, an incidental cystic nephroma was also found. Three other siblings had MNG on surveillance ultrasound examination; two had thyroidectomies, and one had two microscopic foci of papillary carcinoma. Patient A, her mother, and four affected siblings had a germline heterozygous pathogenic DICER1 mutation c.5441C>T in exon 25, resulting in an amino acid change from p.Ser1814Leu of DICER1. Somatic DICER1 RNase IIIb missense mutations were identified in thyroid nodules from three of the four siblings.CONCLUSIONS: This family provides novel insight into an emerging phenotype for DICER1 syndrome, with evidence that germline DICER1 mutations are associated with an increased risk of developing familial DTC, even in the absence of prior treatment with chemotherapy.
|
['Adenocarcinoma', 'Adolescent', 'Carcinoma', 'Carcinoma, Papillary', 'Child', 'DEAD-box RNA Helicases', 'Exons', 'Female', 'Focal Nodular Hyperplasia', 'Humans', 'Male', 'Mutation', 'Mutation, Missense', 'Pedigree', 'Ribonuclease III', 'Thyroid Cancer, Papillary', 'Thyroid Neoplasms', 'Thyroidectomy']
| 26,555,935
|
[['C04.557.470.200.025'], ['M01.060.057'], ['C04.557.470.200'], ['C04.557.470.200.360', 'C04.557.470.700.360'], ['M01.060.406'], ['D08.811.913.696.445.735.720.249'], ['G05.360.340.024.340.137.232'], ['C06.552.270'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G05.365.590'], ['G05.365.590.650'], ['E05.393.673'], ['D08.811.277.352.700.350.707'], ['C04.557.470.200.025.085.612', 'C04.588.322.894.400', 'C04.588.443.915.400', 'C19.344.894.400', 'C19.874.788.400'], ['C04.588.322.894', 'C04.588.443.915', 'C19.344.894', 'C19.874.788'], ['E04.270.856']]
|
['Diseases [C]', 'Named Groups [M]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Analysis of root surface properties by fluorescence/Raman intensity ratio.
|
The aim of this study is to evaluate the existence of residual calculus on root surfaces by determining the fluorescence/Raman intensity ratio. Thirty-two extracted human teeth, partially covered with calculus on the root surface, were evaluated by using a portable Raman spectrophotometer, and a 785-nm, 100-mW laser was applied for fluorescence/Raman excitation. The collected spectra were normalized to the hydroxyapatite Raman band intensity at 960 cm-1. Raman spectra were recorded from the same point after changing the focal distance of the laser and the target radiating angle. In seven teeth, the condition of calculus, cementum, and dentin were evaluated. In 25 teeth, we determined the fluorescence/Raman intensity ratio following three strokes of debridement. Raman spectra collected from the dentin, cementum, and calculus were different. After normalization, spectra values were constant. The fluorescence/Raman intensity ratio of calculus region showed significant differences compared to the cementum and dentin (p < 0.05). The fluorescence/Raman intensity ratio decreased with calculus debridement. For this analysis, the delta value was defined as the difference between the values before and after three strokes, with the final 2 delta values close to zero, indicating a gradual asymptotic curve and the change in intensity ratio approximating that of individual constants. Fluorescence/Raman intensity ratio was effectively used to cancel the angle- and distance-dependent fluctuations of fluorescence collection efficiency during measurement. Changes in the fluorescence/Raman intensity ratio near zero suggested that cementum or dentin was exposed, and calculus removed.
|
['Debridement', 'Dental Calculus', 'Fluorescence', 'Humans', 'Spectrum Analysis, Raman', 'Surface Properties', 'Tooth Root']
| 28,744,587
|
[['E04.176'], ['C07.793.208.250', 'C23.300.175.350'], ['G01.358.500.505.650.665.500', 'G01.590.540.665.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.196.822.860', 'E05.196.867.890'], ['G02.860'], ['A14.549.167.900.750']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Anatomy [A]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Efficacy of Yokukansankachimpihange on sleep disturbance in Parkinson's disease: A study protocol of a randomized, double blind, placebo-controlled pilot trial.
|
INTRODUCTION: Parkinson's disease (PD) is a progressive neurodegenerative disorder that includes motor and nonmotor symptoms. Sleep disturbance is known to decrease the quality of life in patients with PD, and there are limitations to the pharmacotherapy currently in use. Therefore, complementary treatment therapies are required to address these limitations. The traditional herbal medicines Yokukansan (YKS) and Yokukansankachimpihange (YKSCH) have been used to treat insomnia and night crying in children, suggesting their effectiveness against sleep disturbance in patients with PD. We will evaluate whether YKSCH improves sleep disturbance in PD and will identify YKS-related changes in hemodynamic parameters, and neurotransmitter and hormone levels in patients with PD experiencing sleep disturbance.METHODS: We will conduct a randomized, double-blinded, placebo-controlled parallel trial in 34 patients with PD and sleep disturbance, randomly allocating the patients to either placebo-control (n = 17) or treatment groups (n = 17). The total study period will be 16 weeks; administration of YKSCH or placebo, as intervention, will be performed for a 12-week period, and follow-up will be performed over a 4-week period. All subjects will undergo conventional treatment, and be required to maintain a regular medication schedule throughout the study period. The primary outcome measure will be the Scales for Outcomes in PD-Sleep Scale score, and the secondary outcome measures will be polysomnography results, findings from instruments related to sleep disorders, neurotransmitter and hormone levels, and hemodynamic changes in the brain cortex.DISCUSSION AND CONCLUSIONS: This trial will evaluate the effectiveness and safety of YKSCH for sleep improvement in PD with sleep disturbance, and investigate the underlying mechanism of action. We expect improvement in the scores for subjective and objective sleep scales, hemodynamic changes in prefrontal cortical activity, and changes in neurotransmitter and hormone levels. The findings will provide insight into the mechanism underlying the therapeutic effect of YKSCH in PD, and lay the foundation for further studies on whether YKSCH improves sleep disturbance in PD.TRIAL REGISTRATION NUMBER: Clinical Research Information Service (KCT0002869).
|
['Double-Blind Method', 'Drugs, Chinese Herbal', 'Humans', 'Parkinson Disease', 'Polysomnography', 'Prospective Studies', 'Quality of Life', 'Research Design', 'Severity of Illness Index', 'Sleep Wake Disorders']
| 29,953,013
|
[['E05.318.370.300', 'E05.581.500.300', 'N05.715.360.325.320', 'N06.850.520.445.300'], ['D20.215.784.500.350', 'D26.335'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C10.228.140.079.862.500', 'C10.228.662.600.400', 'C10.574.928.750'], ['E01.370.520.625'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['I01.800', 'K01.752.400.750', 'N06.850.505.400.425.837'], ['E05.581.500', 'H01.770.644.728'], ['E05.318.308.980.438.475.456.500', 'N05.715.360.300.800.438.375.364.500', 'N06.850.520.308.980.438.475.364.500'], ['C10.886', 'C23.888.592.796', 'F03.870']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Diseases [C]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Humanities [K]', 'Disciplines and Occupations [H]', 'Psychiatry and Psychology [F]']
| 0
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 1
| 0
|
Thermodynamic analysis of protein-ligand binding interactions in complex biological mixtures using the stability of proteins from rates of oxidation.
|
The detection and quantification of protein-ligand binding interactions is crucial in a number of different areas of biochemical research from fundamental studies of biological processes to drug discovery efforts. Described here is a protocol that can be used to identify the protein targets of biologically relevant ligands (e.g., drugs such as tamoxifen or cyclosporin A) in complex protein mixtures such as cell lysates. The protocol utilizes quantitative, bottom-up, shotgun proteomics technologies (isobaric mass tags for relative and absolute quantification, or iTRAQ) with a covalent labeling technique, termed stability of proteins from rates of oxidation (SPROX). In SPROX, the thermodynamic properties of proteins and protein-ligand complexes are assessed using the hydrogen peroxide-mediated oxidation of methionine residues as a function of the chemical denaturant (e.g., guanidine hydrochloride or urea) concentration. The proteome-wide SPROX experiments described here enable the ligand-binding properties of hundreds of proteins to be simultaneously assayed in the context of complex biological samples. The proteomic capabilities of the protocol render it amenable to the detection of both the on- and off-target effects of ligand binding. The protocol can be completed in 5 d.
|
['Drug Discovery', 'Ligands', 'Oxidation-Reduction', 'Protein Stability', 'Proteins', 'Proteomics', 'Thermodynamics']
| 23,257,983
|
[['E05.295', 'H01.158.703.007.675', 'H01.181.466.675'], ['D27.720.470.480'], ['G02.700', 'G03.295.531'], ['G02.111.700'], ['D12.776'], ['H01.158.201.843', 'H01.158.273.180.350.700', 'H01.158.273.343.350.700', 'H01.181.122.738'], ['G01.906']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
Hyperalgesic and analgesic actions of morphine, U50-488, naltrexone, and (-)-lobeline in the rat brainstem.
|
Morphine, U50-488, and (-)-lobeline produced dose-related shortening of a low-intensity thermally evoked tail avoidance response (LITETAR) (e.g., hyperalgesia) when microinjected into the dorsal posterior mesencephalic tegmentum (DPMT) of conscious rats. The hyperalgesic potency of (-)-lobeline was greater than either morphine or U50-488. With higher doses, morphine's hyperalgesic actions diminished and prolongation of the LITETAR (e.g., analgesia) was observed. Naltrexone produced analgesia in the DPMT that diminished with increasing dose. The hyperalgesic actions of morphine, U50-488, and (-)-lobeline further suggest the presence of kappaergic opioid and nicotinic mechanisms in the DPMT of rats. The hyperalgesic actions of U50-488, a highly specific opioid kappa-receptor agonist, strongly suggest the presence of a kappa-opioidergic hyperalgesic mechanism in the DPMT.
|
['3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer', 'Analgesics', 'Animals', 'Dose-Response Relationship, Drug', 'Female', 'Lobeline', 'Microinjections', 'Morphine', 'Naltrexone', 'Pain', 'Pain Measurement', 'Pyrrolidines', 'Rats', 'Rats, Sprague-Dawley', 'Tegmentum Mesencephali']
| 8,115,422
|
[['D03.383.773.170'], ['D27.505.696.663.850.014', 'D27.505.954.427.040'], ['B01.050'], ['G07.690.773.875', 'G07.690.936.500'], ['D03.132.478', 'D03.383.621.418'], ['E02.319.267.530.690', 'E05.591.570'], ['D03.132.577.249.562.571', 'D03.605.497.607.587', 'D03.633.400.686.607.587', 'D04.615.723.795.576.571'], ['D03.132.577.249.706.550', 'D03.605.497.750.550', 'D03.633.400.686.750.550', 'D04.615.723.795.706.550'], ['C23.888.592.612', 'F02.830.816.444', 'G11.561.790.444'], ['E01.370.600.550.324'], ['D03.383.773'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.750'], ['A08.186.211.132.659.413.875']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Sevoflurane for difficult tracheal intubation.
|
Three patients in whom difficult tracheal intubation was expected but awake fibreoptic intubation was not feasible presented for head and neck surgery. Anaesthesia was induced rapidly and smoothly by inhalation of sevoflurane followed by fibreoptic or conventional tracheal intubation.
|
['Adult', 'Aged', 'Anesthetics, Inhalation', 'Contraindications', 'Ethers', 'Female', 'Head and Neck Neoplasms', 'Humans', 'Intubation, Intratracheal', 'Male', 'Methyl Ethers', 'Middle Aged', 'Parathyroidectomy', 'Sevoflurane']
| 9,389,864
|
[['M01.060.116'], ['M01.060.116.100'], ['D27.505.696.277.100.035.060', 'D27.505.954.427.210.100.035.060'], ['E02.208'], ['D02.355'], ['C04.588.443'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.041.500', 'E02.585.578', 'E05.497.578'], ['D02.355.601'], ['M01.060.116.630'], ['E04.270.694'], ['D02.355.601.810', 'D02.455.526.510.717']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Vitamins and perinatal outcomes among HIV-negative women in Tanzania.
|
BACKGROUND: Prematurity and low birth weight are associated with high perinatal and infant mortality, especially in developing countries. Maternal micronutrient deficiencies may contribute to these adverse outcomes.METHODS: In a double-blind trial in Dar es Salaam, Tanzania, we randomly assigned 8468 pregnant women (gestational age of fetus, 12 to 27 weeks) who were negative for human immunodeficiency virus infection to receive daily multivitamins (including multiples of the recommended dietary allowance) or placebo. All the women received prenatal supplemental iron and folic acid. The primary outcomes were low birth weight (<2500 g), prematurity, and fetal death.RESULTS: The incidence of low birth weight was 7.8% among the infants in the multivitamin group and 9.4% among those in the placebo group (relative risk, 0.82; 95% confidence interval [CI], 0.70 to 0.95; P=0.01). The mean difference in birth weight between the groups was modest (67 g, P<0.001). The rates of prematurity were 16.9% in the multivitamin group and 16.7% in the placebo group (relative risk, 1.01; 95% CI, 0.91 to 1.11; P=0.87), and the rates of fetal death were 4.3% and 5.0%, respectively (relative risk, 0.87; 95% CI, 0.72 to 1.05; P=0.15). Supplementation reduced both the risk of a birth size that was small for gestational age (<10th percentile; 10.7% in the multivitamin group vs. 13.6% in the placebo group; relative risk, 0.77; 95% CI, 0.68 to 0.87; P<0.001) and the risk of maternal anemia (hemoglobin level, <11 g per deciliter; relative risk, 0.88; 95% CI, 0.80 to 0.97; P=0.01), although the difference in the mean hemoglobin levels between the groups was small (0.2 g per deciliter, P<0.001).CONCLUSIONS: Multivitamin supplementation reduced the incidence of low birth weight and small-for-gestational-age births but had no significant effects on prematurity or fetal death. Multivitamins should be considered for all pregnant women in developing countries. (ClinicalTrials.gov number, NCT00197548 [ClinicalTrials.gov].).
|
['Abortion, Spontaneous', 'Adult', 'Ascorbic Acid', 'Birth Weight', 'Double-Blind Method', 'Female', 'Fetal Death', 'HIV Seronegativity', 'Humans', 'Incidence', 'Infant Mortality', 'Infant, Low Birth Weight', 'Infant, Newborn', 'Infant, Small for Gestational Age', 'Pregnancy', 'Pregnancy Outcome', 'Premature Birth', 'Tanzania', 'Vitamin B Complex', 'Vitamin E', 'Vitamins']
| 17,409,323
|
[['C13.703.039', 'G08.686.784.769.496.125'], ['M01.060.116'], ['D02.241.081.844.107', 'D02.241.511.902.107', 'D09.811.100'], ['C23.888.144.186', 'E01.370.600.115.100.160.120.186', 'E05.041.124.160.750.149', 'G07.100.100.160.120.186', 'G07.345.249.314.120.186'], ['E05.318.370.300', 'E05.581.500.300', 'N05.715.360.325.320', 'N06.850.520.445.300'], ['C13.703.223', 'C23.550.260.585'], ['G12.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.308.985.525.375', 'N01.224.935.597.500', 'N06.850.505.400.975.525.375', 'N06.850.520.308.985.525.375'], ['E05.318.308.985.550.475', 'N01.224.935.698.489', 'N06.850.505.400.975.550.475', 'N06.850.520.308.985.550.475'], ['M01.060.703.520.460'], ['M01.060.703.520'], ['M01.060.703.520.460.560'], ['G08.686.784.769'], ['E01.789.700', 'G08.686.784.769.496'], ['C13.703.420.491.500'], ['Z01.058.290.120.840'], ['D27.505.696.494.600.708'], ['D03.383.663.283.909', 'D03.633.100.150.909'], ['D27.505.696.494.600', 'G07.203.300.681.500.600', 'J02.500.681.500.600']]
|
['Diseases [C]', 'Phenomena and Processes [G]', 'Named Groups [M]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Geographicals [Z]', 'Technology, Industry, and Agriculture [J]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 1
| 1
|
Age-related changes in the protein concentration gradient and the crystallin polypeptides of the lens.
|
With the use of a freeze-sectioning technique, the existence of a centripetal protein gradient in the rabbit lens is demonstrated. The steepness of this gradient (the difference in the protein concentration between periphery and the center) varies with age: it increases from a value of 40% in 6- to 8-month-old lenses to 60% by the age of 4 to 5 years and then begins to decline at an average rate of about 5% per year, reaching a value below 40% in 9-year-old lenses. The decline in protein concentration gradient is reciprocated by a gain in the water content. Electrophoretic evidence shows that the decline in the protein gradient is preceded and accompanied by postsynthetic modification of lens crystallin polypeptides. Modifications result in the formation of crosslinked material that stays on the top of sodium dodecyl sulfate gels and of degraded polypeptides with molecular weights below 20,000 daltons. It is hypothesized that the protein gradient comes about probably because lens crystallins are capable of making extensive surface contacts to produce a tightly packed matrix. As the lens ages, post-synthetic modifications and hydrolytic breakdown produce a gradual disorganization in these structural proteins. Local or general disorganization will allow water to fill the gaps and produce hydration, which could predispose the lens to opacity formation.
|
['Adult', 'Aged', 'Aging', 'Animals', 'Body Water', 'Cataract', 'Child, Preschool', 'Crystallins', 'Electrophoresis, Polyacrylamide Gel', 'Humans', 'Hydrolysis', 'Lens, Crystalline', 'Molecular Weight', 'Peptides', 'Rabbits', 'Sodium Dodecyl Sulfate']
| 7,076,406
|
[['M01.060.116'], ['M01.060.116.100'], ['G07.345.124'], ['B01.050'], ['A12.207.200'], ['C11.510.245'], ['M01.060.406.448'], ['D12.776.306.366'], ['E05.196.401.402', 'E05.301.300.319'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G02.380'], ['A09.371.060.500'], ['G02.494'], ['D12.644'], ['B01.050.150.900.649.313.968.700'], ['D02.033.415.220.720', 'D02.886.645.600.055.050.632', 'D10.289.220.720']]
|
['Named Groups [M]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Anatomy [A]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Sodium uptake and membrane excitation in Paramecium.
|
Although the phenotypes of many membrane-excitation mutants of Paramecium are best expressed in Na+-containing solutions, little is known about the role of Na+ in membrane excitation in Paramecium. By measuring 22Na fluxes, we have shown that: (a) The total cellular Na+ content is equivalent to a cytoplasmic concentration of 3--4 mM, if the Na+ concentration is uniform throughout the cell. (b) The kinetics of Na+ uptake can be divided into a saturable Na+ uptake with an apparent Km = 0.15 mM and a nonsaturable Na+ uptake seen at higher Na+ concentrations up to 20 mM. (c) The rate of Na+ uptake in high Na+ solutions is correlated with the duration of backward swimming and membrane excitation in wild type Paramecium and the mutants fast-2 and paranoiac. (d) Na+ uptake is inhibited at 4 degrees C. From these results, we postulate that Na+ uptake is faster when the membrane is depolarized than when it is at the resting potential level.
|
['Animals', 'Cell Membrane', 'Cold Temperature', 'Cytoplasm', 'Electrophysiology', 'Movement', 'Mutation', 'Paramecium', 'Sodium']
| 468,909
|
[]
|
[]
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Regulation of nerve growth factor and nerve growth factor receptor production by NMDA in C6 glioma cells.
|
The synthesis of nerve growth factor (NGF) and nerve growth factor receptor (NGFR) were studied in a C6 glioma cell line by Northern blot hybridization. In response to a glutamate agonist N-methyl-D-aspartic acid (NMDA), NGF mRNA increased by up to 2-fold after 4-12 h of culture. The non-NMDA receptor agonists, quisqualate and kainate, did not induce any increase of NGF mRNA, and kainate actually produced a decrease. The increase in NGF mRNA in response to NMDA was dose-dependent at 1, 5 and 10 microM. NGF receptor (NGFR) mRNA showed changes in expression which were similar to those for NGF mRNA, but were less marked. The specific glutamate antagonist 2-aminophosphonovaleric acid (APV) blocked the increase of NGF mRNA produced by NMDA. In the absence of Ca2+, an increase of NGF mRNA was still observed but in the presence of 1 mM ethylglycol-bis-(beta-aminoethyl ether) N,N'-tetraacetic acid (EGTA), NGF mRNA production abolished. The mechanism producing an increase in NGF mRNA by NMDA may be mediated by cyclic AMP since intracellular cyclic AMP and NGF mRNA levels both increased following treatment with NMDA or dibutyryl cyclic AMP.
|
['2-Amino-5-phosphonovalerate', 'Animals', 'Base Sequence', 'Blotting, Northern', 'Bucladesine', 'Cyclic AMP', 'Glioma', 'Kainic Acid', 'Molecular Sequence Data', 'N-Methylaspartate', 'Neoplasm Proteins', 'Nerve Growth Factors', 'Quisqualic Acid', 'RNA, Messenger', 'RNA, Neoplasm', 'Rats', 'Receptors, Cell Surface', 'Receptors, N-Methyl-D-Aspartate', 'Receptors, Nerve Growth Factor', 'Tumor Cells, Cultured']
| 1,353,854
|
[['D12.125.070.950.100', 'D12.125.740.025'], ['B01.050'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['E05.196.401.095', 'E05.301.300.074', 'E05.601.100'], ['D03.633.100.759.646.138.395.250', 'D13.695.462.200.250', 'D13.695.667.138.395.250', 'D13.695.827.068.395.250'], ['D03.633.100.759.646.138.395', 'D13.695.462.200', 'D13.695.667.138.395', 'D13.695.827.068.395'], ['C04.557.465.625.600.380', 'C04.557.470.670.380', 'C04.557.580.625.600.380'], ['D03.383.773.400'], ['L01.453.245.667'], ['D12.125.067.500.400', 'D12.125.119.170.400'], ['D12.776.624'], ['D12.644.276.860', 'D12.776.467.860', 'D12.776.631.600', 'D23.529.850'], ['D03.383.129.462.580.600', 'D12.125.755'], ['D13.444.735.544'], ['D13.444.735.615'], ['B01.050.150.900.649.313.992.635.505.700'], ['D12.776.543.750'], ['D12.776.157.530.400.400.500.500', 'D12.776.543.550.450.500.200.500', 'D12.776.543.585.400.500.200.500', 'D12.776.543.750.720.200.450.400.500'], ['D12.776.543.750.750.400.550'], ['A11.251.860']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Ultrasonic diagnostic instruments.
|
The underlying physical principles and current limitations of diagnostic ultrasonic instruments are reviewed. Recently developed ultrasonic imaging devices using pulsed-reflected ultrasound are discussed in detail. These instruments transmit short trains of 1.5- to 10-megahertz sound. Echoes reflected from tissue are converted to electrical signals, which are presented on a display device to outline the contour of tissues and organs within the body. The physical resolution of the system is dependent on several design factors in addition to the transmitted sound frequencies. A resolution volume of approximately 1.5 by 3 by 4 millimeters is achieved optimally with commercially available systems operating at 2.25 megahertz. The various instrument designs are described in the context of clinical usage. Because the sound is diffracted, refracted, and reflected, tghe imaging considerations are different from those of x-ray imaging. Diagnostic devices based on the Doppler principle are distinguished from pulsed-reflected ultrasonic instruments.
|
['Auscultation', 'Blood Flow Velocity', 'Echocardiography', 'Heart Valve Diseases', 'Humans', 'Rheology', 'Ultrasonics', 'Ultrasonography']
| 7,423,186
|
[['E01.370.600.060'], ['E01.370.370.130', 'G09.330.380.630.080'], ['E01.370.350.130.750', 'E01.370.350.850.220', 'E01.370.370.380.220'], ['C14.280.484'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.830', 'H01.671.808'], ['H01.671.031.849'], ['E01.370.350.850']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Organisms [B]', 'Disciplines and Occupations [H]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
Wells' syndrome (eosinophilic cellulitis): correlation between clinical activity, eosinophil levels, eosinophil cation protein and interleukin-5.
|
Wells' syndrome (WS) (eosinophilic cellulitis) is characterized by the presence of oedematous skin lesions associated with eosinophilia of the tissues. It has recently been observed that in patients with this disease, increased eosinophil cation protein (ECP) and interleukin (IL) -5 can be detected in peripheral blood, with T lymphocytes that have mRNA for this lymphokine. We present a patient with WS in whom we found a close correlation between clinical activity, eosinophils in blood and bone marrow, and ECP and IL-5 levels in peripheral blood and tissues. We underline the major part played by IL-5 in this disease.
|
['Adult', 'Blood Proteins', 'Cellulitis', 'Eosinophil Granule Proteins', 'Eosinophilia', 'Eosinophils', 'Female', 'Humans', 'Interleukin-5', 'Leukocyte Count', 'Ribonucleases', 'Syndrome']
| 10,215,782
|
[['M01.060.116'], ['D12.776.124'], ['C01.800.130', 'C01.830.200', 'C17.300.185', 'C23.550.470.756.200'], ['D12.776.124.486.379'], ['C15.378.553.231'], ['A11.118.637.415.345', 'A11.627.340.345', 'A15.145.229.637.415.345', 'A15.382.490.315.251'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.644.276.374.465.202', 'D12.776.467.374.465.186', 'D23.529.374.465.202'], ['E01.370.225.500.195.107.595', 'E01.370.225.625.107.595', 'E05.200.500.195.107.595', 'E05.200.625.107.595', 'E05.242.195.107.595', 'G04.140.107.595', 'G09.188.105.595'], ['D08.811.277.352.700'], ['C23.550.288.500']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Anatomy [A]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Marmoricola aquaticus sp. nov., an actinomycete isolated from a marine sponge.
|
A novel marine actinomycete, designated B374(T), was isolated from a marine sponge, Glodia corticostylifera, which was collected from S?o Paulo, Brasil. The taxonomic position of B374(T) was established by using data derived from a polyphasic approach. The organism showed a combination of chemotaxonomic and morphological characteristics consistent with its classification in the genus Marmoricola and it formed a distinct phyletic line in the clade of the genus Marmoricola, based on 16S rRNA gene sequences. Strain B374(T) was most closely related to Marmoricola aequoreus SST-45(T) (98.5% 16S rRNA gene sequence similarity), but was distinguished from this strain and from the other type strains of species of the genus Marmoricola on the basis of a combination of phenotypic properties. The data obtained, therefore, indicates that isolate B374(T) ( = CBMAI 1089(T) = DSM 28169(T)) should be classified as a novel species of the genus Marmoricola, for which the name Marmoricola aquaticus sp. nov. is proposed.
|
['Actinomycetales', 'Animals', 'Base Composition', 'Brazil', 'DNA, Bacterial', 'Fatty Acids', 'Molecular Sequence Data', 'Nucleic Acid Hybridization', 'Phylogeny', 'Porifera', 'RNA, Ribosomal, 16S', 'Sequence Analysis, DNA']
| 26,231,541
|
[['B03.510.024.049'], ['B01.050'], ['G02.111.080'], ['Z01.107.757.176'], ['D13.444.308.212'], ['D10.251'], ['L01.453.245.667'], ['E05.393.661', 'G02.111.611'], ['G05.697', 'G16.075.605', 'L01.100.697'], ['B01.050.500.802'], ['D13.444.735.686.670'], ['E05.393.760.700']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Geographicals [Z]', 'Chemicals and Drugs [D]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 1
|
Influence of the maternal birth status on offspring: a systematic review and meta-analysis.
|
BACKGROUND: Maternal low birth weight (LBW), preterm birth (PTB) and small-for-gestational age (SGA) birth status have been suggested as precursors for an infant being born LBW, PTB or SGA.OBJECTIVE: To systematically review the risks of infant LBW/PTB/SGA among mothers who were LBW/PTB/SGA.SEARCH STRATEGY: Medline, Embase, CINAHL and bibliographies of identified articles were searched for English language studies.SELECTION CRITERIA: Studies reporting association between maternal birth status and LBW, PTB or SGA outcomes were included. Study quality was assessed for biases in selection, exposure assessment, confounder adjustment, analyses, outcomes assessment and attrition.DATA COLLECTION AND ANALYSIS: Unadjusted data from included studies were extracted by two reviewers. Summary odds ratio (OR) and confidence intervals (CI) were calculated using random effect model.MAIN RESULTS: Twenty-two studies of low to moderate risk of biases were included. Maternal LBW was associated with infant LBW (12 studies, 525,706 participants, OR 2.23, 95% CI 2.11-2.35), PTB (six studies, 331,121 participants, OR 1.57, 95% CI 1.43-1.71) and SGA (three studies, 324,357 participants, OR 1.83, 95% CI 1.43-2.33). Maternal PTB was associated with infant PTB (seven studies, 282,616 participants, OR 1.41, 95% CI 1.26-1.59) and SGA (three studies, 41,590 participants, OR 1.33, 95% CI 1.08-1.64). Maternal SGA was associated with infant PTB (OR 1.58, 95% CI 1.06-2.37) and SGA (OR 2.64, 95% CI 2.28-3.05).CONCLUSIONS: Maternal LBW, PTB or SGA status was associated with their children's LBW/PTB/SGA status. Maternal birth status should be included in risk assessment when planning pregnancy care for individual women.
|
['Female', 'Humans', 'Infant, Newborn', 'Infant, Small for Gestational Age', 'Mothers', 'Pregnancy', 'Premature Birth', 'Quantitative Trait, Heritable']
| 19,916,879
|
[['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703.520'], ['M01.060.703.520.460.560'], ['F01.829.263.500.320.200', 'I01.880.853.150.500.340.270', 'M01.620.630'], ['G08.686.784.769'], ['C13.703.420.491.500'], ['G05.420.720']]
|
['Organisms [B]', 'Named Groups [M]', 'Psychiatry and Psychology [F]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Phenomena and Processes [G]', 'Diseases [C]']
| 0
| 1
| 1
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
|
A porohyperelastic finite element model of the eye: the influence of stiffness and permeability on intraocular pressure and optic nerve head biomechanics.
|
Progressively deteriorating visual field is a characteristic feature of primary open-angle glaucoma (POAG), and the biomechanics of optic nerve head (ONH) is believed to be important in its onset. We used porohyperelasticity to model the complex porous behavior of ocular tissues to better understand the effect variations in ocular material properties can have on ONH biomechanics. An axisymmetric model of the human eye was constructed to parametrically study how changes in the permeabilities of retina-Bruch's-choroid complex (k(RBC)), sclera k(sclera), uveoscleral pathway (k(UVSC)) and trabecular meshwork k(TM) as well as how changes in the stiffness of the lamina cribrosa (LC) and sclera affect IOP, LC strains, and translaminar interstitial pressure gradients (TLIPG). Decreasing k(RBC) from 5 ? 10(- 12) to 5 ? 10(- 13) m/s increased IOP and LC strains by 17%, and TLIPG by 21%. LC strains increased by 13% and 9% when the scleral and LC moduli were decreased by 48% and 50%, respectively. In addition to the trabecular meshwork and uveoscleral pathway, the retina-Bruch's-choroid complex had an important effect on IOP, LC strains, and TLIPG. Changes in k(RBC) and scleral modulus resulted in nonlinear changes in the IOP, and LC strains especially at the lowest k(TM) and k(UVSC). This study demonstrates that porohyperelastic modeling provides a novel method for computationally studying the biomechanical environment of the ONH. Porohyperelastic simulations of ocular tissues may help provide further insight into the complex biomechanical environment of posterior ocular tissues in POAG.
|
['Biomechanical Phenomena', 'Finite Element Analysis', 'Glaucoma', 'Humans', 'Intraocular Pressure', 'Models, Theoretical', 'Optic Disk', 'Permeability', 'Sclera', 'Trabecular Meshwork']
| 26,195,024
|
[['G01.154.090', 'G01.374.089'], ['E05.355'], ['C11.525.381'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G14.440'], ['E05.599'], ['A08.800.800.120.680.660', 'A09.371.729.690'], ['G02.723'], ['A09.371.784'], ['A09.371.060.932']]
|
['Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Organisms [B]', 'Anatomy [A]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Profiles of a healthful diet and its relationship to biomarkers in a population sample from Mediterranean southern France.
|
OBJECTIVES: The failure of single-nutrient supplementation to prevent disease in intervention studies underlines the necessity to develop a holistic view of food intake. The objectives of this study were to devise a diet quality index (DQI) and identify biomarkers of multidimensional dietary behavior.DESIGN: A nutrition survey was conducted in Mediterranean southern France by means of a food frequency questionnaire. The DQI was based on current dietary recommendations for prevention of diet-related diseases such as cardiovascular disease and some cancers. A second DQI included tobacco use.STATISTICAL ANALYSES: performed Spearman rank correlations, cross-classifications and intraclass correlations were computed between the DQI and biomarkers.RESULTS: Of the 146 subjects, 10 had a healthful diet and 18 had a poor diet. Erythrocyte omega-3 fatty acids-eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)-beta carotene, and vitamin E concentrations were lower and cholesterol concentrations were higher in the poor diet; the difference was significant for EPA and DHA and borderline significant for vitamin E. Significant correlation was found between the DQI and vitamin E (-0.12), EPA (-0.30), and DHA (-0.28), and beta carotene (-0.17) when tobacco use was considered, but not between the DQI and cholesterol. The correlation coefficient reached 0.58 (P0.01) for a composite index based on all biomarkers except cholesterol.CONCLUSIONS: Subjects with a beta carotene levels greater thanl micromol/L, vitamin E greater than 30 micromol/L and EPA greater than 0.65% and DHA greater than 4% of fatty acids in erythrocytes were likely to have a healthful diet. Each biomarker indicated the quality of diet, but correlation was higher with a composite index.
|
['Adult', 'Aged', 'Biomarkers', 'Cholesterol', 'Diet', 'Docosahexaenoic Acids', 'Eicosapentaenoic Acid', 'Erythrocytes', 'Female', 'France', 'Humans', 'Male', 'Middle Aged', 'Nutrition Surveys', 'Statistics, Nonparametric', 'Vitamin E', 'beta Carotene']
| 11,043,701
|
[['M01.060.116'], ['M01.060.116.100'], ['D23.101'], ['D04.210.500.247.222.284', 'D04.210.500.247.808.197', 'D10.570.938.208'], ['G07.203.650.240'], ['D10.212.302.380.410.210', 'D10.251.355.337.250', 'D10.627.430.450.375'], ['D10.212.302.380.410.385', 'D10.251.355.255.200', 'D10.251.355.337.290', 'D10.627.430.450.390'], ['A11.118.290', 'A11.443.240', 'A15.145.229.334'], ['Z01.542.286'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['E05.318.308.980.485', 'N05.715.360.300.800.469', 'N06.850.505.616', 'N06.850.520.308.980.469'], ['E05.318.740.995', 'N05.715.360.750.760', 'N06.850.520.830.995'], ['D03.383.663.283.909', 'D03.633.100.150.909'], ['D02.455.326.271.665.202.123', 'D02.455.426.392.368.367.379.249.050', 'D02.455.849.131.123', 'D23.767.261.050']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Geographicals [Z]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
E-selectin polymorphism associated with myocardial infarction causes enhanced leukocyte-endothelial interactions under flow conditions.
|
OBJECTIVE: Polymorphisms found in genes encoding adhesion molecules have been reported to be associated with atherosclerosis. We investigated the Ser128Arg polymorphism in the E-selectin gene in Japanese patients with myocardial infarction and its functional significance.METHODS AND RESULTS: Results from 135 patients with myocardial infarction and 327 control subjects revealed that the frequency of Arg128-positive was significantly higher in the patients than in controls (12.6% versus 6.7%; odds ratio, 2.0; 95% CI, 1.04 to 3.85), indicating that the Ser128Arg polymorphism was associated with myocardial infarction. We then generated a recombinant E-selectin adenovirus carrying a mutation (AdS128R-E) and compared it with its wild-type counterpart by evaluating the adhesion characteristics of transduced human umbilical vein endothelial cells under flow. AdS128R-E-transduced human umbilical vein endothelial cells supported significantly more rolling and adhesion of neutrophils and mononuclear cells compared with human umbilical vein endothelial cells transduced with AdWT-E (P<0.001) and also exhibited significantly greater levels of phosphorylation of extracellular signal regulated kinase 1 and 2 and p38 mitogen-activated protein kinase, suggesting that an altered endothelial signaling pathway is associated with this polymorphism.CONCLUSIONS: Our results suggest that the E-selectin Ser128Arg polymorphism can functionally alter leukocyte-endothelial interactions as well as biochemical and biological consequences, which may account for the pathogenesis of myocardial infarction.
|
['Adult', 'Aged', 'Amino Acid Substitution', 'Cell Adhesion', 'Cells, Cultured', 'Chemotaxis, Leukocyte', 'E-Selectin', 'Endothelial Cells', 'Endothelium, Vascular', 'Female', 'Gene Frequency', 'Humans', 'Japan', 'Male', 'Middle Aged', 'Mitogen-Activated Protein Kinase 1', 'Mitogen-Activated Protein Kinase 3', 'Mitogen-Activated Protein Kinases', 'Monocytes', 'Mutation, Missense', 'Myocardial Infarction', 'Neutrophils', 'Point Mutation', 'Polymorphism, Genetic', 'Recombinant Fusion Proteins', 'Rheology', 'Transduction, Genetic', 'p38 Mitogen-Activated Protein Kinases']
| 12,649,084
|
[['M01.060.116'], ['M01.060.116.100'], ['E05.393.420.601.035', 'G05.558.109'], ['G04.022'], ['A11.251'], ['G04.198.424.233'], ['D12.776.395.550.200.700.300', 'D12.776.503.843.300', 'D12.776.543.550.200.700.300', 'D23.050.301.350.700.300'], ['A11.436.275'], ['A07.015.700.500', 'A10.272.491.355'], ['G05.330'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['Z01.252.474.463', 'Z01.639.595'], ['M01.060.116.630'], ['D08.811.913.696.620.682.700.567.249.500', 'D12.644.360.450.169.500', 'D12.776.476.450.169.500'], ['D08.811.913.696.620.682.700.567.249.750', 'D12.644.360.450.169.750', 'D12.776.476.450.169.750'], ['D08.811.913.696.620.682.700.567', 'D12.644.360.450', 'D12.776.476.450'], ['A11.118.637.555.652', 'A11.148.580', 'A11.627.624', 'A11.733.547', 'A15.145.229.637.555.652', 'A15.378.316.580', 'A15.382.490.555.652', 'A15.382.670.547', 'A15.382.680.547'], ['G05.365.590.650'], ['C14.280.647.500', 'C14.907.585.500', 'C23.550.513.355.750', 'C23.550.717.489.750'], ['A11.118.637.415.583', 'A11.627.340.583', 'A11.733.689', 'A15.145.229.637.415.583', 'A15.382.490.315.583', 'A15.382.680.689'], ['G05.365.590.675'], ['G05.365.795'], ['D12.776.828.300'], ['E05.830', 'H01.671.808'], ['E05.393.350.800', 'G05.728.850'], ['D08.811.913.696.620.682.700.567.843', 'D12.644.360.450.835', 'D12.776.476.450.835']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Geographicals [Z]', 'Diseases [C]', 'Disciplines and Occupations [H]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 1
| 0
| 1
|
Seasonality, smoking and history of poor treatment compliance are strong predictors of dropout in a naturalistic 6 year follow-up of bipolar patients.
|
Bipolar disorder is a highly recurrent disease which requires long-term treatment. Dropout is a major problem, poorly understood. The objectives of this study were to know the risk of dropout of a cohort of bipolar patients under ambulatory treatment and to identify the clinical profile of patients more likely to abandon the follow-up. A sample of 285 BD I and II patients was followed up for a mean of 2.87 years. A significant proportion of patients failed regular follow-up. The dropout rates were 6.3 % at three months, 12.7 % at 6 months, and 17.6, 27.2, 37.3, 44.0, 47.2 and 49.0 % at 1, 2, 3, 4, 5 and 6 years respectively. Very few variables at baseline predicted dropout. Patients under 35 years of age were more likely to dropout than older cases. Seasonality, smoking and specially history of poor treatment compliance were strong predictors of dropout. Given the magnitude of dropout, additional early clinical interventions should be considered for high-risk patients.
|
['Adult', 'Age Factors', 'Aged', 'Bipolar Disorder', 'Female', 'Humans', 'Kaplan-Meier Estimate', 'Longitudinal Studies', 'Male', 'Middle Aged', 'Patient Compliance', 'Patient Dropouts', 'Predictive Value of Tests', 'Retrospective Studies', 'Risk Factors', 'Seasons', 'Smoking', 'Time Factors']
| 24,986,371
|
[['M01.060.116'], ['N05.715.350.075', 'N06.850.490.250'], ['M01.060.116.100'], ['F03.084.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.740.998.650', 'N05.715.360.750.795.650', 'N06.850.520.830.998.650'], ['E05.318.372.500.750.500', 'N05.715.360.330.500.750.500', 'N06.850.520.450.500.750.500'], ['M01.060.116.630'], ['F01.100.150.750.500.600', 'F01.145.488.887.500.600', 'N05.300.150.800.500.600'], ['F01.100.150.750.500.610', 'F01.145.488.887.500.610', 'N05.300.150.800.500.610'], ['E05.318.370.800.650', 'N05.715.360.325.700.640', 'N06.850.520.445.800.650'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['G01.910.645.661', 'G16.500.275.071.590', 'N06.230.300.100.250.525'], ['F01.145.805'], ['G01.910.857']]
|
['Named Groups [M]', 'Health Care [N]', 'Psychiatry and Psychology [F]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Phase I Study of the Investigational Aurora A Kinase Inhibitor Alisertib plus Rituximab or Rituximab/Vincristine in Relapsed/Refractory Aggressive B-cell Lymphoma.
|
PURPOSE: The aurora A kinase inhibitor alisertib demonstrated single-agent clinical activity and preclinical synergy with vincristine/rituximab in B-cell non-Hodgkin lymphoma (B-NHL). This phase I study aimed to determine the safety and recommended phase II dose (RP2D) of alisertib in combination with rituximab ± vincristine in patients with relapsed/refractory aggressive B-NHL.PATIENTS AND METHODS: Patients with relapsed/refractory, diffuse, large, or other aggressive B-NHL received oral alisertib 50 mg b.i.d. days 1 to 7, plus i.v. rituximab 375 mg/m2 on day 1, for up to eight 21-day cycles (MR). Patients in subsequent cohorts (3 + 3 design) received increasing doses of alisertib (30 mg starting dose; 10 mg increments) b.i.d. days 1 to 7 plus rituximab and vincristine [1.4 mg/m2 (maximum 2 mg) days 1, 8] for 8 cycles (MRV). Patients benefiting could continue single-agent alisertib beyond 8 cycles. Cell-of-origin and MYC/BCL2 IHC was performed on available archival tissue.RESULTS: Forty-five patients participated. The alisertib RP2D for MR was 50 mg b.i.d. For MRV (n = 32), the RP2D was determined as 40 mg b.i.d. [1 dose-limiting toxicity (DLT) at 40 mg; 2 DLTs at 50 mg]. Drug-related adverse events were reported in 89% of patients, the most common was neutropenia (47%). Seven patients had complete responses (CR), 7 had partial responses (PRs); 9 of 20 (45%) patients at the MRV RP2D responded (4 CRs, 5 PRs), all with non-germinal center B-cell (GCB) diffuse large B-cell lymphoma (DLBCL).CONCLUSIONS: The combination of alisertib 50 mg b.i.d. plus rituximab or alisertib 40 mg b.i.d. plus rituximab and vincristine was well tolerated and demonstrated activity in non-GCB DLBCL.
|
['Adult', 'Aged', 'Aged, 80 and over', 'Antineoplastic Combined Chemotherapy Protocols', 'Aurora Kinase A', 'Azepines', 'Disease Progression', 'Drug Monitoring', 'Drug Resistance, Neoplasm', 'Female', 'Humans', 'Lymphoma, B-Cell', 'Male', 'Maximum Tolerated Dose', 'Middle Aged', 'Molecular Targeted Therapy', 'Neoplasm Metastasis', 'Neoplasm Staging', 'Pyrimidines', 'Recurrence', 'Retreatment', 'Rituximab', 'Treatment Outcome', 'Vincristine']
| 30,082,475
|
[['M01.060.116'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['E02.183.750.500', 'E02.319.077.500', 'E02.319.310.037'], ['D08.811.913.696.620.682.700.103.500', 'D12.776.167.049.500'], ['D03.383.066'], ['C23.550.291.656'], ['E01.370.520.200'], ['G07.690.773.984.395'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C04.557.386.480.150', 'C15.604.515.569.480.150', 'C20.683.515.761.480.150'], ['E05.940.481', 'G07.690.936.625'], ['M01.060.116.630'], ['E02.319.574'], ['C04.697.650', 'C23.550.727.650'], ['E01.789.625'], ['D03.383.742'], ['C23.550.291.937'], ['E02.887'], ['D12.776.124.486.485.114.224.075.785', 'D12.776.124.790.651.114.224.075.785', 'D12.776.377.715.548.114.224.284.785'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['D03.132.436.681.827.817', 'D03.633.100.473.402.681.827.817', 'D03.633.100.496.500.500.681.827.817']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Health Care [N]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Microdermabrasion: a clinical, histometric, and histopathologic study.
|
BACKGROUND: Microdermabrasion is a nonchemical superficial resurfacing procedure that removes the stratum corneum. Because it is a somewhat subtle procedure, it may be difficult to demonstrate its cosmetic benefit.OBJECTIVES: To assess the efficacy of microdermabrasion in treatment of melasma, postacne scarring, striae distensae, and photoaging by a series of microdermabrasion sittings through clinical, histometric, and histopathologic analysis.PATIENTS AND METHODS: The study was conducted on 38 patients constituting four groups (melasma, acne scars, striae destinsae, and photoaging), each patient was subjected to a series of 8 microdermabrasion treatments performed at 1-week interval. Punch biopsies, at base line, 1 week after the 4th and the 8th session, were obtained from each subject for histometric and histopathologic analysis.RESULTS: Clinically, the improvement in melasma, acne scars, and striae distensae groups was mainly mild to moderate, while in photoaging group was mainly mild. Histometric analysis of epidermal thickness showed insignificant changes in all groups. Histopathologically, decreased melanization and regular distribution of melanosomes in the epidermis were noted in melasma group while increased density of collagen fibers with more regular arrangement in collagen bundles was detected in all candidates of acne scars, striae distensae, and photoaging groups. Meanwhile, Orcein stain did not show any significant changes in elastic fibers.CONCLUSIONS: Microdermabrasion is an easy and safe technique. In this study, most cases showed mild to moderate improvement. Decreased melanization with regular distribution of melanosomes and increased collagen density with regular arrangement of collagen bundles were the most common observed histologic changes.
|
['Acne Vulgaris', 'Adolescent', 'Adult', 'Aged', 'Biopsy', 'Cicatrix', 'Dermabrasion', 'Dermis', 'Epidermis', 'Female', 'Humans', 'Male', 'Melanosis', 'Middle Aged', 'Skin Aging', 'Striae Distensae', 'Young Adult']
| 27,357,600
|
[['C17.800.030.150', 'C17.800.794.111'], ['M01.060.057'], ['M01.060.116'], ['M01.060.116.100'], ['E01.370.225.500.384.100', 'E01.370.225.998.054', 'E01.370.388.100', 'E04.074', 'E05.200.500.384.100', 'E05.200.998.054', 'E05.242.384.100'], ['A10.165.450.300', 'C23.550.355.274', 'G16.762.891.249'], ['E02.218.210', 'E04.680.275.250'], ['A17.815.180'], ['A10.272.497', 'A17.815.250'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C17.800.621.430.530'], ['M01.060.116.630'], ['G13.750.804'], ['C23.888.885.800'], ['M01.060.116.815']]
|
['Diseases [C]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Intracellular Fas ligand in normal and malignant breast epithelium does not induce apoptosis in Fas-sensitive cells.
|
Fas ligand (FasL) is expressed on some cancers and may play a role in the immune evasion of the tumour. We used immuno-histochemistry to study the expression of Fas and FasL in tissue samples from breast cancer patients, as well as normal breast tissue. Our results show that Fas and FasL are co-expressed both in normal tissue and in breast tumours. Fas and FasL mRNA were expressed in fresh normal and malignant breast tissue, as well as cultured breast epithelium and breast cancer cell lines. Flow cytometry analysis of live cells failed to detect FasL on the surface of normal or malignant breast cells; however, both stained positive for FasL after permeabilization. Fas was detected on the surface of normal breast cells and T47D and MCF-10A cell lines but only intracellularly in other breast cell lines tested. Neither normal breast epithelium nor breast cell lines induced Fas-dependent apoptosis in Jurkat cells. Finally, 20 tumour samples were stained for apoptosis. Few apoptotic cells were detected and there was no increase in apoptotic cells on the borders between tumour cells and lymphocytes. We conclude that FasL is expressed intracellularly in both normal and malignant breast epithelium and unlikely to be important for the immune evasion of breast tumours.
|
['Animals', 'Apoptosis', 'Blotting, Western', 'Breast', 'Breast Neoplasms', 'Cells, Cultured', 'Epithelium', 'Fas Ligand Protein', 'Female', 'Flow Cytometry', 'Gene Expression Regulation', 'Humans', 'Immunohistochemistry', 'Jurkat Cells', 'Membrane Glycoproteins', 'RNA', 'Reverse Transcriptase Polymerase Chain Reaction', 'Tumor Cells, Cultured', 'fas Receptor']
| 11,104,571
|
[['B01.050'], ['G04.146.954.035'], ['E05.196.401.143', 'E05.301.300.096', 'E05.478.566.320.200', 'E05.601.262', 'E05.601.470.320.200'], ['A01.236'], ['C04.588.180', 'C17.800.090.500'], ['A11.251'], ['A10.272'], ['D12.644.276.374.750.249', 'D12.776.395.550.312', 'D12.776.467.374.750.249', 'D12.776.543.550.312', 'D23.529.374.750.249'], ['E01.370.225.500.363.342', 'E01.370.225.500.386.350', 'E05.196.712.516.600.240.350', 'E05.200.500.363.342', 'E05.200.500.386.350', 'E05.242.363.342', 'E05.242.386.350'], ['G05.308'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.225.500.607.512', 'E01.370.225.750.551.512', 'E05.200.500.607.512', 'E05.200.750.551.512', 'E05.478.583', 'H01.158.100.656.234.512', 'H01.158.201.344.512', 'H01.158.201.486.512', 'H01.181.122.573.512', 'H01.181.122.605.512'], ['A11.251.210.190.495', 'A11.251.860.180.495', 'A15.382.490.555.567.569.440'], ['D12.776.395.550', 'D12.776.543.550'], ['D13.444.735'], ['E05.393.620.500.725'], ['A11.251.860'], ['D12.776.543.750.690.500', 'D12.776.543.750.705.852.760.195']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Disciplines and Occupations [H]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
Preliminary experience and development of an algorithm for the optimal use of the laparoscopic component separation technique for myofascial advancement during ventral incisional hernia repair.
|
BACKGROUND: Component separation technique (CST) enables rectus abdominus medialization, but may cause wound complications. Few published outcomes exist involving laparoscopic CST. Our aim was to examine feasibility and outcomes involving open and laparoscopic (lap) CST during ventral incisional hernia repair (VIHR) and present an algorithm for ventral herniorrhaphy.STUDY DESIGN: Our design was a retrospective cohort study. Over 22 months, 28 patients underwent one of the following: (i) unilateral (U-) lap CST with open VIHR [n = 5], (ii) bilateral (B-) lap CST with open VIHR [n = 7], (iii) B-lap CST with lap VIHR [n = 8], or (iv) B-open CST with open VIHR [n = 8]. Indications for open VIHR included mesh removal, concomitant visceral procedure, wound revision, thin/ulcerated skin, abdominal wall tumor, frozen abdomen, and/or off-midline hernia. During open VIHR, CST was performed in the Ramirez fashion. Lap CST was performed before intraperitoneal access in lap VIHR and after retrorectus dissection in open VIHR. Patient surveillance consisted of clinical encounters and telephone interviews.RESULTS: Groups were similar regarding age, body mass index, American Society of Anesthesiologists classification, hernia width, operative time, and hospital stay. Six of the 20 patients who underwent open VIHR developed wound complications, and two required early reoperation. Four of the six with concomitant visceral procedures had wound complications. No laparoscopic VIHR patients had a wound complication. Based on 11 months' follow-up, one open VIHR patient has concern for recurrence.CONCLUSIONS: Laparoscopic CST is feasible during open and laparoscopic VIHR, but it appears most beneficial for wound healing after laparoscopic VIHR. During open VIHR, laparoscopic CST may not substantially reduce wound complications.
|
['Abdominal Wall', 'Aged', 'Algorithms', 'Cohort Studies', 'Feasibility Studies', 'Female', 'Hernia, Ventral', 'Humans', 'Laparoscopy', 'Male', 'Middle Aged', 'Retrospective Studies']
| 21,524,200
|
[['A01.923.047.050'], ['M01.060.116.100'], ['G17.035', 'L01.224.050'], ['E05.318.372.500.750', 'N05.715.360.330.500.750', 'N06.850.520.450.500.750'], ['E05.318.372.550', 'E05.337.675', 'N05.715.360.330.550', 'N06.850.520.450.550'], ['C23.300.707.374.937'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.388.250.520', 'E04.502.250.520'], ['M01.060.116.630'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825']]
|
['Anatomy [A]', 'Named Groups [M]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Diseases [C]', 'Organisms [B]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 1
| 1
| 0
|
Mn2+ exerts stronger structural effects than the Mn-citrate complex on the human erythrocyte membrane and molecular models.
|
While traces of manganese (Mn) take part in important and essential functions in biology, elevated exposures have been shown to cause significant toxicity. Chronic exposure to the metal leads to manganese neurotoxicity (or manganism), a brain disorder that resembles Parkinsonism. Toxic effect mechanisms of Mn is not understood, toxic concentrations of manganese are not well defined and blood manganese concentration at which neurotoxicity occurs has not been identified. There are reports indicating that the most abundant Mn-species in Mn carriers within blood is the Mn-citrate complex. Despite the well-documented information about the toxic effects of Mn, there are scarce reports concerning the effects of manganese compounds on both structure and functions of cell membranes, particularly those of human erythrocytes. With the aim to better understand the molecular mechanisms of the interaction of Mn with cell membranes, MnCl(2), and the Mn-citrate complex were incubated with intact erythrocytes, isolated unsealead human erythrocyte membranes (IUM), and molecular models of the erythrocyte membrane. These consisted in bilayers of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylethanolamine (DMPE), phospholipid classes present in the outer and inner monolayers of the erythrocyte membrane, respectively. The capacity of the Mn compounds to perturb the bilayer structures of DMPC and DMPE was evaluated by X-ray diffraction, IUM were studied by fluorescence spectroscopy, and intact human erythrocytes were observed by scanning electron microscopy (SEM). In all these systems it was found that Mn(2+) exerted considerable higher structural perturbations than the Mn-citrate complex.
|
['Citrates', 'Dimyristoylphosphatidylcholine', 'Erythrocyte Membrane', 'Humans', 'Manganese', 'Microscopy, Electron, Scanning', 'Models, Molecular', 'Phosphatidylethanolamines', 'X-Ray Diffraction']
| 19,880,186
|
[['D02.241.081.901.434'], ['D10.570.755.375.760.400.800.200'], ['A11.118.290.270', 'A11.284.149.356', 'A15.145.229.334.270'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D01.268.556.484', 'D01.268.956.374', 'D01.552.544.484'], ['E01.370.350.515.402.541', 'E05.595.402.541'], ['E05.599.595'], ['D10.570.755.375.760.400.840'], ['E05.196.309.742', 'E05.196.822.950', 'G01.867.950', 'G02.965']]
|
['Chemicals and Drugs [D]', 'Anatomy [A]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Directed evolution of bicyclic peptides for therapeutic application.
|
Many naturally occurring cyclic peptides or derivatives thereof are used as therapeutics such as the human hormones vasopressin and oxytocin or the antibiotics vancomycin and daptomycin. The success of cyclic peptide therapeutics is based on their ability to bind with high affinity, their good target selectivity and their low toxicity. As nature provides cyclic peptides to only a small number of disease targets, strategies have been developed to generate cyclic peptide ligands with tailored specificity de novo. Our laboratory is specialized on the directed evolution of bicyclic peptide ligands by phage display. In this article, we review our recent work to in vitro evolve bicyclic peptide antagonists, the binding and pharmacokinetic properties of bicyclic peptides, as well as efforts to generate bicyclic peptides for therapeutic application.
|
['Directed Molecular Evolution', 'Half-Life', 'Peptides, Cyclic']
| 24,594,337
|
[['E05.393.420.175'], ['G01.910.405'], ['D04.345.566', 'D12.644.641']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Does active referral by a doctor or 12-Step peer improve 12-Step meeting attendance? Results from a pilot randomised control trial.
|
BACKGROUND: Active engagement in 12-Step self-help groups (SHG) is associated with improvements in substance use outcomes during and after treatment, yet levels of participation in SHG meetings in the U.K. remain low.METHOD: An RCT investigating the impact of active referral to SHG, delivered by doctors or 12-Step peers during inpatient treatment on both inpatient and post-treatment meeting attendance was conducted. 151 inpatients with alcohol, opiate, crack-cocaine or benzodiazepine dependence undergoing detoxification, received one of two active referral interventions: 12-Step peer intervention (PI), doctor intervention (DI), or no intervention (NI). 83% of the sample was followed up, 2-3 months following discharge.RESULTS: Active referral interventions significantly increased attendance at 12-Step meetings during inpatient treatment (88% versus 73%, p<.05). Rates of post-discharge meeting attendance were PI=64%, DI=48%, NI=33%, with those in the PI group significantly (OR=3.6; CI=1.3, 9.8) more likely to have attended. Inpatient meeting attenders were three times as likely to have attended meetings post-discharge (59% versus 20%, p<.01), and post-discharge meeting attenders reported significantly higher abstinence rates (60.8% versus 39.2%, p<.05) at follow-up. However, abstinence rates did not differ significantly across intervention groups (44% [PI], 41% [DI] and 36% [NI]).CONCLUSION: Attendance at 12-Step SHGs is associated with greater rates of abstinence and active referral, especially by 12-Step peers, increases 12-Step SHG attendance rates. However, improved clinical outcomes among attendees might not occur until stronger recovery peer support networks are established. These results show promise for the benefit of incorporating Twelve Step Facilitation into U.K. treatment settings.
|
['Adult', 'Alcoholism', 'Benzodiazepines', 'Cocaine-Related Disorders', 'Demography', 'Diagnostic and Statistical Manual of Mental Disorders', 'Female', 'Humans', 'Male', 'Middle Aged', 'Opioid-Related Disorders', 'Patient Acceptance of Health Care', 'Patient Compliance', 'Physicians', 'Pilot Projects', 'Psychiatric Status Rating Scales', 'Referral and Consultation', 'Self-Help Groups', 'Substance-Related Disorders']
| 22,677,458
|
[['M01.060.116'], ['C25.775.100.250', 'F03.900.100.350'], ['D03.633.100.079.080'], ['C25.775.300', 'F03.900.300'], ['I01.240', 'N01.224', 'N06.850.505.400'], ['L01.453.245.945.200'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['C25.775.643.500', 'F03.900.647.500'], ['F01.100.150.750.500', 'F01.145.488.887.500', 'N05.300.150.800.500'], ['F01.100.150.750.500.600', 'F01.145.488.887.500.600', 'N05.300.150.800.500.600'], ['M01.526.485.810', 'N02.360.810'], ['E05.318.372.750', 'E05.337.737', 'N05.715.360.330.720', 'N06.850.520.450.720'], ['F04.711.513.653'], ['N04.452.758.849'], ['N03.540.782'], ['C25.775', 'F03.900']]
|
['Named Groups [M]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Chemicals and Drugs [D]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]', 'Information Science [L]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 1
| 1
| 0
|
Amino acid pairs susceptible to variants in human protein C precursor.
|
In this study, we analyze the amino acid pairs in human protein C precursor to determine which amino acid pairs are more susceptible to 71 variants from missense mutant human protein C precursor. The results show 85.92% of 71 variants occur at randomly unpredictable amino acid pairs accounting for 61.96% of amino acid pairs in protein C.
|
['Amino Acid Substitution', 'Forecasting', 'Genetic Variation', 'Humans', 'Mutation, Missense', 'Protein C', 'Protein Precursors', 'Thrombophilia']
| 16,029,164
|
[['E05.393.420.601.035', 'G05.558.109'], ['I01.320'], ['G05.365'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G05.365.590.650'], ['D08.622.705', 'D12.776.124.650', 'D12.776.395.635', 'D12.776.811.243.705', 'D23.113.700'], ['D12.776.811'], ['C15.378.925']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
|
Angiotensin II influences ovarian follicle development in the transgenic (mRen-2)27 and Sprague-Dawley rat.
|
There is accumulating evidence that local renin-angiotensin systems (RASs) influence cell growth and organ function in a variety of tissues including the ovary. The first aim of this study was to characterise the cellular location of RAS components in the rat ovary. This was facilitated by the use of the hypertensive transgenic (mRen-2)27 rat which overexpresses renin and angiotensin in extra-renal tissues. Comparisons were made with normal Sprague-Dawley (SD) rats. The second aim was to determine if the upregulated RAS of the transgenic (mRen-2)27 rat and infusion of angiotensin II (ANG II) in SD rats influences follicle number and litter size. Gene expression, immunohistochemical and autoradiographic techniques were used to identify a discrete RAS including ANG II receptors in the ovarian stroma, follicles (particularly atretic) and to a lesser extent corpora lutea. The RAS at these sites was most abundant in homozygous (HMZ) followed by heterozygous (HTZ) (mRen-2)27 rats and then SD rats. Large antral and preovulatory follicles and litter size were reduced in (mRen-2)27 rats. In HMZ (mRen-2)27 rats and SD rats infused with ANG II, angiotensin 1a (AT(1a)) receptor mRNA in the ovarian stroma was lower than control SD rats and was associated with a reduction in large antral and preovulatory follicles. These findings indicate that upregulation of the ovarian RAS in the rat influences follicular development and, potentially, reproductive capacity.
|
['Angiotensin II', 'Animals', 'Animals, Genetically Modified', 'Female', 'Hypertension', 'Immunohistochemistry', 'In Situ Hybridization', 'Litter Size', 'Ovarian Follicle', 'Peptidyl-Dipeptidase A', 'Pregnancy', 'RNA, Messenger', 'Rats', 'Rats, Sprague-Dawley', 'Receptor, Angiotensin, Type 1', 'Renin']
| 14,765,984
|
[['D06.472.699.094.078', 'D12.644.400.070.078', 'D12.644.456.073.041', 'D12.644.548.058.078', 'D12.776.631.650.070.078', 'D23.469.050.050.050'], ['B01.050'], ['B01.050.050.136', 'B05.620.136'], ['C14.907.489'], ['E01.370.225.500.607.512', 'E01.370.225.750.551.512', 'E05.200.500.607.512', 'E05.200.750.551.512', 'E05.478.583', 'H01.158.100.656.234.512', 'H01.158.201.344.512', 'H01.158.201.486.512', 'H01.181.122.573.512', 'H01.181.122.605.512'], ['E01.370.225.500.620.670.325', 'E01.370.225.750.600.670.325', 'E05.200.500.620.670.325', 'E05.200.750.600.670.325', 'E05.393.661.475'], ['G08.686.530', 'G08.686.784.769.498.300'], ['A05.360.319.114.630.535', 'A06.300.312.497.535'], ['D08.811.277.656.350.350.687'], ['G08.686.784.769'], ['D13.444.735.544'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.750'], ['D12.776.543.750.695.047.625', 'D12.776.543.750.750.130.750'], ['D08.811.277.656.074.500.780', 'D08.811.277.656.300.048.780', 'D08.811.277.656.837.750']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Phenomena and Processes [G]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
Genome sequence of Enterococcus hirae (Streptococcus faecalis) ATCC 9790, a model organism for the study of ion transport, bioenergetics, and copper homeostasis.
|
Enterococcus hirae ATCC 9790 is a Gram-positive lactic acid bacterium that has been used in basic research for over 4 decades. Here we report the sequence and annotation of the 2.8-Mb genome of E. hirae and its endemic 29-kb plasmid pTG9790.
|
['Copper', 'DNA, Bacterial', 'Energy Metabolism', 'Enterococcus faecalis', 'Genome, Bacterial', 'Homeostasis', 'Ion Transport', 'Molecular Sequence Data', 'Plasmids', 'Sequence Analysis, DNA']
| 22,933,757
|
[['D01.268.556.195', 'D01.268.956.170', 'D01.552.544.195'], ['D13.444.308.212'], ['G03.295'], ['B03.353.750.250.250.280', 'B03.510.550.250.250.280'], ['G05.360.340.358.207'], ['G07.410'], ['G03.143.500'], ['L01.453.245.667'], ['G05.360.600'], ['E05.393.760.700']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Construction of multiform scFv antibodies using linker peptide.
|
Multiform single chain variable fragments (scFvs) including different length linker scFvs and bispecific scFv were constructed. The linker lengths of 0, 3, 5, 8, 12, and 15 amino acids between V(H) and V(L) of antideoxynivalenol (anti-DON) scFv were used to analyze the affinities of scFvs. The affinity constants of these scFvs increased when the linker was lower than 12 amino acids. The affinity constant would not change when the linker was longer than 12 amino acids. Fusion gene of anti-DON scFv and antizearalenone (anti-ZEN) scFv was also constructed through connection by a short peptide linker DNA to express a bispecific scFv. The affinity constants assay showed that the two scFvs of fusion bispecific scFv remained their own affinity compared to their parental scFvs. Competitive direct enzyme linked immunosorbent assay was used to detect DON and ZEN in contaminated wheat (Triticum aestivum L.) samples, and the results indicated that this bispecific scFv was applicable in DON and ZEN detection. This work confirmed that bispecific scFv could be successfully obtained, and might also have an application in diagnosing fungal infection, and breeding transgenic plants.
|
['Antibodies', 'Antibodies, Bispecific', 'Antibody Affinity', 'Base Sequence', 'Cloning, Molecular', 'Enzyme-Linked Immunosorbent Assay', 'Escherichia coli', 'Food Contamination', 'Gene Expression', 'Immunoglobulin Variable Region', 'Peptides', 'Trichothecenes', 'Triticum', 'Zearalenone']
| 18,499,076
|
[['D12.776.124.486.485.114', 'D12.776.124.790.651.114', 'D12.776.377.715.548.114'], ['D12.776.124.486.485.114.125', 'D12.776.124.790.651.114.134', 'D12.776.377.715.548.114.134'], ['G12.040', 'G12.122.125'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['E05.393.220'], ['E05.478.566.350.170', 'E05.478.566.380.360', 'E05.478.583.400.170', 'E05.601.470.350.170', 'E05.601.470.380.360'], ['B03.440.450.425.325.300', 'B03.660.250.150.180.100'], ['J01.576.423.850.730.500.249', 'N06.850.460.400', 'N06.850.601.500.249'], ['G05.297'], ['D12.644.541.500.650.500', 'D12.776.124.486.485.680.650.500', 'D12.776.124.486.485.797', 'D12.776.124.790.651.680.650.500', 'D12.776.124.790.651.797', 'D12.776.377.715.548.680.650.500', 'D12.776.377.715.548.797', 'G02.111.570.060.425'], ['D12.644'], ['D02.455.849.765.850', 'D04.345.891', 'D23.946.587.933'], ['B01.650.940.800.575.912.250.822.918'], ['D02.455.426.559.389.657.852.900', 'D02.540.950', 'D23.946.587.989']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Technology, Industry, and Agriculture [J]', 'Health Care [N]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 1
| 0
|
Responder characteristics to a single oral dose of cholinesterase inhibitor: a double-blind placebo-controlled study with tacrine in Alzheimer patients.
|
A proportion of Alzheimer's disease (AD) patients treated for several months with cholinesterase (ChE) inhibitors have shown some favorable response on cognition, but the characteristics of the responders are still unclear. This study attempts to identify the characteristics of individuals with a positive behavioral response after a double-blind randomized administration of a single oral dose of tacrine (40 mg) and placebo to AD patients. Furthermore, the relationship between single-dose and long-term responders are examined. Twenty-four mildly to very mildly demented AD patients participated in the study. They all fulfilled the diagnosis of probable AD according to NINCDS-ADRDA criteria. Active treatment (tacrine 40 mg) and placebo was administered in random order on 2 consecutive days, and the effects were evaluated within 2 h using neuropsychological tests (assessing visuospatial ability, episodic memory and attention), registration of EEG activity and measurement of red blood cells (RBC) acetylcholinesterase (AChE), ChE activity and concentrations of tacrine and its metabolites in plasma. Results demonstrated significant improvement, tacrine compared to placebo, in measures of attention, but not in episodic memory or visuospatial ability. A single-dose response was therefore defined in terms of improvement in attention. The tacrine plasma concentration (pcTHA) showed a positively skewed distribution (mean +/- SD: 10.5 +/- 11.8, range: 1.0-51.8 ng/ml). There were no significant differences between single-dose responders compared to nonresponders in pcTHA, metabolites of tacrine, inhibition of AChE in RBC, tau levels in CSF, AChE activity in CSF or plasma and demographic variables. However, single-dose responders showed a higher right frontal alpha/theta ratio on EEG and had lower glucose metabolism in the parietal-temporal association cortex at baseline. In addition, the frequency of apolipoprotein E (APOE) epsilon 4 alleles was higher in responders. Interestingly, the single-dose response was related to the long-term response, although not significantly, which probably was due to lack of power. To conclude, the present study identified single-dose responders in terms of improved attentional performance associated with a relatively higher alpha/theta activity in the right frontal regions of the brain measured on EEG and predominance of APOE epsilon 4 allele.
|
['Acetylcholine', 'Administration, Oral', 'Aged', 'Alleles', 'Alzheimer Disease', 'Apolipoprotein E4', 'Apolipoproteins E', 'Attention', 'Brain', 'Cholinesterase Inhibitors', 'Cognition', 'Cross-Over Studies', 'Double-Blind Method', 'Electroencephalography', 'Female', 'Humans', 'Male', 'Middle Aged', 'Neuropsychological Tests', 'Severity of Illness Index', 'Tacrine', 'Tomography, Emission-Computed']
| 11,125,238
|
[['D02.092.211.111'], ['E02.319.267.100'], ['M01.060.116.100'], ['G05.360.340.024.340.030'], ['C10.228.140.380.100', 'C10.574.945.249', 'F03.615.400.100'], ['D10.532.091.500.750', 'D12.776.070.400.500.750', 'D12.776.521.120.500.750'], ['D10.532.091.500', 'D12.776.070.400.500', 'D12.776.521.120.500'], ['F02.830.104.214'], ['A08.186.211'], ['D27.505.519.389.275', 'D27.505.519.625.120.300', 'D27.505.696.577.120.300'], ['F02.463.188'], ['E05.318.370.150', 'N05.715.360.325.150', 'N06.850.520.445.150'], ['E05.318.370.300', 'E05.581.500.300', 'N05.715.360.325.320', 'N06.850.520.445.300'], ['E01.370.376.300', 'E01.370.405.245'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['F04.711.513'], ['E05.318.308.980.438.475.456.500', 'N05.715.360.300.800.438.375.364.500', 'N06.850.520.308.980.438.475.364.500'], ['D03.633.300.046.250.900'], ['E01.370.350.350.800', 'E01.370.350.600.350.800', 'E01.370.350.710.800', 'E01.370.350.825.800', 'E01.370.384.730.800']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Named Groups [M]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Anatomy [A]', 'Health Care [N]', 'Organisms [B]']
| 1
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Dissemination and implementation: INQRI's potential impact.
|
BACKGROUND: Application of research evidence in care delivery improves patient outcomes. Large gaps still exist, however, between recommended care and that used in practice. To increase the understanding of implementation studies, and dissemination of research findings, we present the perspective of investigators from seven Interdisciplinary Nursing Quality Research Initiative (INQRI)-funded studies.OBJECTIVE: To describe implementation strategies, challenges, and lessons learned from conducting 5 INQRI-funded implementation studies, and present 2 case examples of other INQRI studies to illustrate dissemination strategies. Potential impact of study findings are set forth.RESEARCH DESIGN: Qualitative descriptive methods were used for the implementation studies. Case examples were set forth by investigators using reflection questions.RESULTS: Four of the 5 implementation studies focused on clinical topics and 1 on professional development of nurse managers, 4 were multisite studies. Common implementation strategies used across studies addressed education, ongoing interaction with sites, use of implementation tools, and visibility of the projects on the study units. Major challenges were the Institutional Review Board approval process and the short length of time allocated for implementation. Successes and lessons learned included creating excitement about research, packaging of study tools and resources for use by other organizations, and understanding the importance of context when conducting this type of research. Case examples revealed that study findings have been disseminated to study sites and to the health care community through publications and presentations. The potential impact of all 7 studies is far reaching.CONCLUSIONS: This study captures several nuanced perspectives from 5 Principal Investigators, who were completing INQRI-funded implementation studies. These nuanced perspectives are important lessons for other scientists embarking on implementation studies. The INQRI case examples illustrate important dissemination strategies and impact of findings on quality of care.
|
['Accidental Falls', 'Assisted Living Facilities', 'Evidence-Based Practice', 'Foundations', 'Geriatric Nursing', 'Humans', 'Motor Activity', "Nurse's Role", 'Nursing Evaluation Research', 'Organizational Case Studies', 'Outcome and Process Assessment, Health Care', 'Patient-Centered Care', 'Personnel Staffing and Scheduling', 'Quality Improvement', 'Quality of Health Care', 'Research', 'Research Support as Topic', 'United States']
| 23,502,916
|
[['N06.850.135.122'], ['J03.775.187', 'N02.278.825.187'], ['H02.249'], ['N03.219.483.311', 'N03.540.630.180'], ['H02.478.676.236', 'N02.421.533.245'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F01.145.632', 'G11.427.410.698'], ['F01.829.316.616.625.450', 'N05.300.100.337'], ['H01.770.644.145.390.432', 'H02.478.395.432', 'N04.590.233.508.613.432'], ['N03.349.380.710', 'N05.715.360.455'], ['N04.761.559', 'N05.715.360.575'], ['N04.590.233.727.407'], ['I03.946.225', 'N04.452.677.650'], ['J01.293.754', 'N04.761.744'], ['N04.761', 'N05.715'], ['H01.770.644'], ['N03.219.483.645'], ['Z01.107.567.875']]
|
['Health Care [N]', 'Technology, Industry, and Agriculture [J]', 'Disciplines and Occupations [H]', 'Organisms [B]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Geographicals [Z]']
| 0
| 1
| 0
| 0
| 0
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 1
|
Safety of local anaesthesia in dental patients taking oral anticoagulants: is it still controversial?
|
The aim of this study was to investigate the safety of local infiltration techniques and the inferior alveolar nerve block (IANB) in dental patients taking oral anticoagulants. A total of 352 patients were given a total of 560 injections of local anaesthetic (119 IANB and 441 others). The study group comprised 279 patients with therapeutic international normalised ratios (INRs), and the control group 73 patients who were taking oral anticoagulants but had subtherapeutic INR on the day of operation. Blood was aspirated 7 times (7.3%) during the IANB in the study group. However, there were no clinical signs of prolonged haemorrhage into the medial pterygoid muscle or pterygomandibular space after 96 IANB, including those from whom blood had been aspirated. Only two minor haematomas developed after multiple infiltrations in the lingual sulci. The results suggest that bleeding as a result of the use of local anaesthesia in patients with therapeutic INR is unlikely, provided that the IANB is done correctly.
|
['Administration, Oral', 'Adult', 'Aged', 'Aged, 80 and over', 'Anesthesia, Dental', 'Anesthesia, Local', 'Anesthetics, Local', 'Anticoagulants', 'Female', 'Follow-Up Studies', 'Hematoma', 'Hemorrhage', 'Humans', 'International Normalized Ratio', 'Lidocaine', 'Longitudinal Studies', 'Male', 'Mandibular Nerve', 'Maxilla', 'Mepivacaine', 'Middle Aged', 'Mouth Floor', 'Nerve Block', 'Oral Hemorrhage', 'Patient Safety', 'Prospective Studies', 'Pterygoid Muscles', 'Young Adult']
| 21,130,546
|
[['E02.319.267.100'], ['M01.060.116'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['E03.155.141', 'E06.045'], ['E03.155.086.231'], ['D27.505.696.277.100.200', 'D27.505.696.663.850.025', 'D27.505.954.427.210.100.200'], ['D27.505.954.502.119'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['C23.550.414.838'], ['C23.550.414'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.225.625.115.320', 'E05.200.625.115.320'], ['D02.065.199.092.500', 'D02.092.146.113.092.500'], ['E05.318.372.500.750.500', 'N05.715.360.330.500.750.500', 'N06.850.520.450.500.750.500'], ['A08.800.800.120.760.500'], ['A02.835.232.781.324.502.645', 'A14.521.645'], ['D03.383.621.450'], ['M01.060.116.630'], ['A14.549.441'], ['E03.155.086.711', 'E04.525.210.550'], ['C07.465.625', 'C23.550.414.922', 'C23.888.619.500'], ['N06.850.135.060.075.399'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['A02.633.567.600.700', 'A14.530.790'], ['M01.060.116.815']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Named Groups [M]', 'Chemicals and Drugs [D]', 'Health Care [N]', 'Diseases [C]', 'Organisms [B]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Expression of Glut-1 is a prognostic marker for oral squamous cell carcinoma patients.
|
Oral squamous cell carcinoma (OSCC) is among the tenth most common human cancers worldwide with evidence of an increase in incidence rate and mortality. Despite advances in treatment modalities, the prognosis of this cancer is still very poor and has not changed over the past two decades. This study is based on samples collected from 42 patients with a primary OSCC. Immunohistochemical staining for Glut-1 was carried out and compared with the clinicopathological data. Thirty-two patients showed in their tumors a weak or undetectable Glut-1 expression, whereas in tumors of 10 patients a moderate to strong Glut-1 expression was detected. In multivariate Cox's regression hazard analysis, patients whose tumors had a moderate to strong Glut-1 expression possessed a 4.9-fold increased risk of tumor-related death compared to the other patients. Our results suggest that Glut-1 expression is an independent prognostic marker for routine assessment of OSCC.
|
['Adult', 'Aged', 'Aged, 80 and over', 'Carcinoma, Squamous Cell', 'Female', 'Gene Expression Regulation, Neoplastic', 'Glucose Transporter Type 1', 'Humans', 'Immunohistochemistry', 'Male', 'Middle Aged', 'Mouth Neoplasms', 'Prognosis', 'Proportional Hazards Models', 'Retrospective Studies']
| 19,020,718
|
[['M01.060.116'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['C04.557.470.200.400', 'C04.557.470.700.400'], ['G05.308.370'], ['D12.776.157.530.500.500.500', 'D12.776.157.530.937.563.500', 'D12.776.543.585.500.500.500', 'D12.776.543.585.937.625.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.225.500.607.512', 'E01.370.225.750.551.512', 'E05.200.500.607.512', 'E05.200.750.551.512', 'E05.478.583', 'H01.158.100.656.234.512', 'H01.158.201.344.512', 'H01.158.201.486.512', 'H01.181.122.573.512', 'H01.181.122.605.512'], ['M01.060.116.630'], ['C04.588.443.591', 'C07.465.530'], ['E01.789'], ['E05.318.740.500.700', 'E05.318.740.600.700', 'E05.318.740.750.725', 'E05.318.740.998.825', 'E05.599.835.900', 'N05.715.360.750.530.650', 'N05.715.360.750.625.650', 'N05.715.360.750.695.650', 'N05.715.360.750.795.825', 'N06.850.520.830.500.700', 'N06.850.520.830.600.700', 'N06.850.520.830.750.725', 'N06.850.520.830.998.912'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825']]
|
['Named Groups [M]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Health Care [N]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 1
| 1
| 0
|
Micro-RNA quantification using DNA polymerase and pyrophosphate quantification.
|
A rapid quantification method for micro-RNA based on DNA polymerase activity and pyrophosphate quantification has been developed. The tested micro-RNA serves as the primer, unlike the DNA primer in all DNA sequencing methods, and the DNA probe serves as the template for DNA replication. After the DNA synthesis, the pyrophosphate detection and quantification indicate the existence and quantity of the tested miRNA. Five femtomoles of the synthetic RNA could be detected. In 20-100 ìg RNA samples purified from SiHa cells, the measurement was done using the proposed assay in which hsa-miR-16 and hsa-miR-21 are 0.34 fmol/ìg RNA and 0.71 fmol/ìg RNA, respectively. This simple and inexpensive assay takes less than 5 min after total RNA purification and preparation. The quantification is not affected by the pre-miRNA which cannot serve as the primer for the DNA synthesis in this assay. This assay is general for the detection of the target RNA or DNA with a known matched DNA template probe, which could be widely used for detection of small RNA, messenger RNA, RNA viruses, and DNA. Therefore, the method could be widely used in RNA and DNA assays.
|
['Base Sequence', 'Biochemistry', 'Biological Assay', 'Cell Line, Tumor', 'Chemical Fractionation', 'DNA-Directed DNA Polymerase', 'Diphosphates', 'Humans', 'Limit of Detection', 'MicroRNAs', 'Molecular Sequence Data', 'Reference Standards']
| 21,910,961
|
[['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['H01.158.201', 'H01.181.122'], ['E05.091'], ['A11.251.210.190', 'A11.251.860.180'], ['E05.196.155'], ['D08.811.913.696.445.308.300'], ['D01.029.260.700.675.374.775.150', 'D01.248.497.158.730.650.200', 'D01.695.625.675.650.775.150'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.740.872.374', 'N05.715.360.750.725.500', 'N06.850.520.830.872.500'], ['D13.150.650.319', 'D13.444.735.150.319', 'D13.444.735.790.552.500'], ['L01.453.245.667'], ['E05.978.808']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Disciplines and Occupations [H]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Health Care [N]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 0
|
Views on death and dying among health care workers in an Indian cancer care hospice: balancing individual and collective perspectives.
|
In providing palliative and end-of-life care, professional and lay hospice workers alike attend to patient and family needs to encourage a dignified death. However, there are few comparative inquiries documenting how differential workplace preparation affects the processes and outcomes related to being confronted to death and dying. This qualitative study explores and compares these experiences among a diverse sample of health workers (N = 25) in a grassroots cancer care hospice in Bangalore, India. Our findings underscore how personal views, socio-economic status, beliefs and values, occupational experience, and workplace interventions interact to shape 'worldviews' about death and dying. Whereas health workers report conflicting feelings of relief and sadness when confronted with the death of their patients, these mixed emotions are often lessened through open dialogue among newly trained and more experienced health workers. Moreover, experienced hospice workers wished to ensure that less experienced ones are provided with the necessary workplace support to lessen psychological 'hardening' that may occur with repeated exposure to death. In dealing with the diverse needs of hospice workers, both individual and collective needs must be considered to ensure an optimal workplace climate. Future work should study how hospice workers' views on death and dying evolve with time and experience.
|
['Adaptation, Psychological', 'Adolescent', 'Attitude to Death', 'Culture', 'Evaluation Studies as Topic', 'Female', 'Health Personnel', 'Humans', 'India', 'Interviews as Topic', 'Male', 'Professional-Patient Relations', 'Social Class', 'Social Values', 'Spirituality', 'Stress, Psychological', 'Terminal Care', 'Young Adult']
| 21,464,118
|
[['F01.058'], ['M01.060.057'], ['F01.100.125', 'N05.300.125'], ['I01.076.201.450', 'I01.880.853.100'], ['E05.337', 'N05.715.360.335'], ['M01.526.485', 'N02.360'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['Z01.252.245.393'], ['E05.318.308.420', 'L01.399.250.520', 'N05.715.360.300.400', 'N06.850.520.308.420'], ['F01.829.401.650', 'N05.300.660'], ['I01.880.853.996.755', 'N01.824.782'], ['F01.829.873'], ['F02.880.705', 'K01.844.664.500'], ['F01.145.126.990', 'F02.830.900'], ['E02.760.905', 'N02.421.585.905'], ['M01.060.116.815']]
|
['Psychiatry and Psychology [F]', 'Named Groups [M]', 'Health Care [N]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Geographicals [Z]', 'Information Science [L]', 'Humanities [K]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 1
| 1
| 1
|
Insulin sensitizing actions of fenugreek seed polyphenols, quercetin & metformin in a rat model.
|
BACKGROUND & OBJECTIVE: Plant polyphenols have been known to exert anti-diabetic action and promote insulin action. The present study was carried out to compare the effects of administration of fenugreek seed polyphenolic extract (FPEt), quercetin and metformin (a positive control) in an acquired model of insulin resistance (IR).METHODS: Adult male Wistar rats divided into seven groups (n=12). IR was induced in groups (groups 2, 3, 4 and 5) by feeding a high fructose diet (FRU) (60 g/100 g diet) for 60 days. From day 16, FRU rats were administered either FPEt (200 mg/kg bw) (group 3), quercetin (50mg/kg bw) (group 4) or metformin (50 mg/kg bw) (group 5) for the next 45 days. Group 1 served as normal control while groups 6 and 7 served as FPEt and quercetin controls respectively. Oral glucose tolerance test (OGTT) was done on day 59 to assess glucose tolerance. At the end of 60 days, the levels of glucose, insulin, triglycerides (TG) and free fatty acids (FFA) were measured in the blood and the activities of insulin-inducible and suppressible enzymes in cytosolic and mitochondrial fractions of liver and skeletal muscle. The extent of tyrosine phosphorylation of proteins in response to insulin was determined by assaying protein tyrosine kinase (PTK) and protein tyrosine phosphatase (PTP) in liver.RESULTS: Fructose caused increased levels of glucose, insulin, TG and FFA, alterations in insulin sensitivity indices, enzyme activities and reduced glycogen content. Higher PTP activity and lower PTK activity suggest reduced tyrosine phosphorylation status. Administration of FPEt or quercetin improved insulin sensitivity and tyrosine phosphorylation in fructose-fed animals and the effect was comparable with that of metformin.INTERPRETATION & CONCLUSION: Our findings indicated that FPEt and quercetin improved insulin signaling and sensitivity and thereby promoted the cellular actions of insulin in this model.
|
['Animals', 'Blood Glucose', 'Fatty Acids, Nonesterified', 'Flavonoids', 'Fructose', 'Glucose Tolerance Test', 'Insulin', 'Insulin Resistance', 'Liver', 'Male', 'Metformin', 'Phenols', 'Plant Extracts', 'Polyphenols', 'Protein Tyrosine Phosphatases', 'Protein-Tyrosine Kinases', 'Quercetin', 'Rats', 'Rats, Wistar', 'Seeds', 'Triglycerides', 'Trigonella']
| 19,535,835
|
[['B01.050'], ['D09.947.875.359.448.500'], ['D10.251.310'], ['D03.383.663.283.266.450', 'D03.633.100.150.266.450'], ['D09.947.875.359.250', 'D09.947.875.465.354'], ['E01.370.225.124.100.355', 'E01.370.374.355', 'E05.200.124.100.355'], ['D06.472.699.587.200.500.625', 'D12.644.548.586.200.500.625'], ['C18.452.394.968.500', 'G07.690.773.984.617'], ['A03.620'], ['D02.078.370.141.450'], ['D02.455.426.559.389.657'], ['D20.215.784.500', 'D26.667'], ['D02.455.426.559.389.657.715', 'D03.633.100.150.266.450.260.777'], ['D08.811.277.352.650.775'], ['D08.811.913.696.620.682.725'], ['D03.383.663.283.266.450.284.777', 'D03.633.100.150.266.450.284.777'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.900'], ['A18.024.500.750', 'G07.203.300.775', 'J02.500.775'], ['D10.351.801'], ['B01.650.940.800.575.912.250.401.937']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Technology, Industry, and Agriculture [J]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
[Locally advanced prostatic cancer and systemic involvement].
|
In a group of 115 patients with prostatic cancer, the correlation between the local and systemic evolution of the neoplasm has been evaluated. In 80 patients non local and systemic up-staging of the tumor was observed. In further 18 patients both local and systemic staging uppers and in the remaining 17 patients no correlation between local and systemic evolution was observed. In conclusion, in this series, the prostatic cancer demonstrated an high concordance in both local and systemic evolution and then the modifications of local staging can be used as a predictive parameter of systemic evolution of the prostatic cancer.
|
['Follow-Up Studies', 'Humans', 'Male', 'Neoplasm Metastasis', 'Neoplasm Staging', 'Prostatic Neoplasms']
| 7,889,057
|
[['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C04.697.650', 'C23.550.727.650'], ['E01.789.625'], ['C04.588.945.440.770', 'C12.294.260.750', 'C12.294.565.625', 'C12.758.409.750']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Ventricular dysrhythmias following an alfentanil anesthetic in a patient on reserpine for hypertension.
|
The case is presented of a hypertensive patient on reserpine, who developed ventricular irritability after administration of alfentanil, a new ultra-short-acting narcotic used in anesthesia. The dysrhythmias resolved after cessation of the offending agent. There are no reported cases of interaction of reserpine and alfentanil in the literature.
|
['Alfentanil', 'Anesthesia, Intravenous', 'Arrhythmias, Cardiac', 'Bradycardia', 'Cardiac Complexes, Premature', 'Drug Interactions', 'Female', 'Humans', 'Hypertension', 'Middle Aged', 'Reserpine', 'Tachycardia', 'Ventricular Function']
| 1,722,376
|
[['D03.383.621.265.100'], ['E03.155.308'], ['C14.280.067', 'C23.550.073'], ['C14.280.067.319', 'C23.550.073.300'], ['C14.280.067.325', 'C14.280.123.375', 'C23.550.073.325'], ['G07.690.773.968'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C14.907.489'], ['M01.060.116.630'], ['D03.132.436.681.933.500', 'D03.633.100.473.402.681.933.500', 'D03.633.100.496.500.500.681.933.500'], ['C14.280.067.845', 'C14.280.123.875', 'C23.550.073.845'], ['G09.330.955']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Named Groups [M]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Increasing health insurance coverage in the first year of life.
|
OBJECTIVES: To determine the proportion of infants who are uninsured and the sociodemographic characteristics of their mothers, including prenatal and post-partum insurance coverage, in order to identify strategies to increase infant health coverage.METHODS: Data from the 2001 California Maternal and Infant Health Assessment (MIHA) were analyzed. MIHA is a cross-sectional survey of a statewide representative sample of 3,475 postpartum women. We calculated the proportion of uninsured infants overall and by several maternal characteristics. Adjusted and unadjusted odds ratios for infant uninsurance are reported.RESULTS: In the overall study sample, 8.7% of infants were uninsured. Low-income infants were significantly more likely to be uninsured than infants in households with incomes above 200% of the federal poverty level (13.7% vs. 2.5%). The mother's prenatal and post-partum health coverage, her age, and family income were associated with an increased risk of infant uninsurance after adjustment for other maternal characteristics. A large majority of the uninsured infants (88.1%) were living in low-income families. The mothers of 60% of the uninsured infants were enrolled in Medicaid during the pregnancy.CONCLUSIONS: Approximately 14% of California's low-income infants were uninsured at the time of the survey despite being income-eligible for Medicaid. The proportion of uninsured infants could potentially be reduced by more than one-half through strategies to provide 12 month continuous enrollment of infants with federally mandated Medicaid eligibility for the first year of life.
|
['Adolescent', 'Adult', 'California', 'Cross-Sectional Studies', 'Female', 'Humans', 'Infant', 'Infant, Newborn', 'Insurance Coverage', 'Insurance, Health', 'Medicaid', 'Poverty']
| 16,328,706
|
[['M01.060.057'], ['M01.060.116'], ['Z01.107.567.875.580.200', 'Z01.107.567.875.760.200'], ['E05.318.372.500.875', 'N05.715.360.330.500.875', 'N06.850.520.450.500.875'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703'], ['M01.060.703.520'], ['N03.219.521.576.265'], ['N03.219.521.576.343'], ['N03.219.521.346.506.564.655', 'N03.706.615.693'], ['I01.880.735.634', 'I01.880.853.996.535', 'N01.824.600']]
|
['Named Groups [M]', 'Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Anthropology, Education, Sociology, and Social Phenomena [I]']
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 1
|
Closure of the laryngeal vestibule during deglutition.
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With the aid of high-speed cineradiography (up to 100 frames per second) the closure of the laryngeal vestibule during deglutition was studied in 150 volunteers free from swallowing complaints. The closure was observed to be effected by a peristaltic-like wave from below. This was accomplished in two steps. First the caudal segment of the vestibule, the supraglottic space, was closed by an apposition of the side walls. Then the cranial segment of the vestibule, the supraglottic space, was closed by an apposition of the fixed part of the epiglottis to the arytenoid region. The subdivision of the vestibule was made by an imaginary plane between the interarytenoid incisure and incisure of the thyroid cartilage. This subdivision corresponds to the location of the thyroarytenoid muscles lateral to the supraglottic space and the thyroepiglottic muscles lateral to the subepiglottic space. In this way the two muscle pairs contribute indifferent and distinct ways to the closure of the vestibule during deglutition.
|
['Adult', 'Aged', 'Cineradiography', 'Deglutition', 'Female', 'Humans', 'Laryngeal Muscles', 'Larynx', 'Male', 'Middle Aged', 'Muscles', 'Pharynx']
| 7,064,688
|
[['M01.060.116'], ['M01.060.116.100'], ['E01.370.350.700.225.469'], ['G10.261.178'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A02.633.567.500', 'A04.329.604'], ['A04.329'], ['M01.060.116.630'], ['A02.633', 'A10.690'], ['A03.556.750', 'A04.623', 'A14.724']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Anatomy [A]']
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Genetic factors influencing prostate cancer risk in Norwegian men.
|
Norway has one of the highest rates of death due to prostate cancer (PCa) in the world. To assess the contribution of both common and rare single nucleotide variants (SNPs) to the prostate cancer burden in Norway, we assessed the frequency of the established prostate cancer susceptibility allele, HOXB13 G84E, as well as a series of validated, common PCa risk SNPs in a Norwegian PCa population of 779 patients. The G84E allele was observed in 2.3% of patients compared to 0.7% of control individuals, OR = 3.8, P = 1 ? 10-4. While there was a trend toward an earlier age at diagnosis, overall the clinicopathologic features of PCa were not significantly different in G84E carriers and non-carriers. Evaluation of 32 established common risk alleles revealed significant associations of risk alleles at 13 loci, including SNPs at 8q24, and near TET2, SLC22A3, NKX3-1, CASC8, MYC, DAP2IP, MSMB, HNF1B, PPP1R14A, and KLK2/3. When the data for each SNP are combined into a genetic risk score (GRS), Norwegian men within the top decile of GRS have over 5-fold greater risk to be diagnosed with PCa than men with GRS in the lowest decile. These results indicate that risk alleles of HOXB13 and common variant SNPs are important components of inherited PCa risk in the Norwegian population, although these factors appear to contribute little to the malignancy's aggressiveness.
|
['Adult', 'Aged', 'Alleles', 'Gene Frequency', 'Genetic Predisposition to Disease', 'Genotype', 'Germ-Line Mutation', 'Homeodomain Proteins', 'Humans', 'Male', 'Middle Aged', 'Norway', 'Polymorphism, Single Nucleotide', 'Prostate', 'Prostatic Neoplasms']
| 29,181,843
|
[['M01.060.116'], ['M01.060.116.100'], ['G05.360.340.024.340.030'], ['G05.330'], ['C23.550.291.687.500', 'G05.380.355'], ['G05.380'], ['G05.365.590.350'], ['D12.776.260.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['Z01.542.816.374'], ['G05.365.795.598'], ['A05.360.444.575', 'A10.336.707'], ['C04.588.945.440.770', 'C12.294.260.750', 'C12.294.565.625', 'C12.758.409.750']]
|
['Named Groups [M]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Geographicals [Z]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 1
|
The tropics: cradle, museum or casino? A dynamic null model for latitudinal gradients of species diversity.
|
Several ecological and evolutionary hypotheses have been proposed to explain the latitudinal diversity gradient (LDG), but a general model for this conspicuous pattern remains elusive. Mid-domain effect (MDE) models generate gradients of species diversity by randomly placing the geographic ranges of species in one- or two-dimensional spaces, thus excluding both evolutionary processes and the effect of contemporary climate. Traditional MDE models are statistical and static because they determine the size of ranges either randomly or based on empirical frequency distributions. Here we present a simple dynamic null model for the LDG that simulates stochastic processes of range shifts, extinction and speciation. The model predicts higher species diversity and higher extinction and speciation rates in the tropics, and a strong influence of range movements in shaping the LDG. These null expectations should be taken into consideration in studies aimed at understanding the many factors that generate latitudinal diversity gradients.
|
['Biodiversity', 'Extinction, Biological', 'Geography', 'Models, Biological', 'Tropical Climate']
| 18,445,032
|
[['G16.500.275.157.049', 'N06.230.124.049'], ['G16.510'], ['H01.277.500'], ['E05.599.395'], ['G16.500.275.071.600', 'N06.230.300.100.250.600']]
|
['Phenomena and Processes [G]', 'Health Care [N]', 'Disciplines and Occupations [H]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 0
| 0
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
|
Diabetes mellitus reduces the antiarrhythmic effect of ion channel blockers.
|
We designed the present study to examine whether diabetes mellitus affects the antiarrhythmic effect of flecainide, a sodium channel blocker, E-4031, a potassium channel blocker, and verapamil, a calcium channel blocker, in diabetic rats. The experiments were performed in intact and diabetic rats 2, 4, and 6 wk after administration of streptozotocin. Rats were anesthetized with halothane and monitored continuously for arterial blood pressure and premature ventricular contractions. The arrhythmogenic dose of epinephrine was defined as the smallest dose producing 3 or more premature ventricular contractions within a 15-s period. The arrhythmogenic doses of epinephrine in the presence of flecainide were 8.2 +/- 2.2 (mean +/- sd), 7.4 +/- 6.1, 5.5 +/- 2.8, and 2.0 +/- 0.5 microg/kg in intact and diabetic rats 2, 4, and 6 wk after streptozotocin administration, respectively. Similarly, the arrhythmogenic doses of epinephrine in the presence of E-4031 were 7.7 +/- 2.6, 2.3 +/- 0.7, 2.0 +/- 0.7, and 1.2 +/- 0.5 microg/kg, and those in the presence of verapamil were 8.2 +/- 2.1, 3.1 +/- 1.2, 2.3 +/- 0.9, and 1.5 +/- 0.5 microg/kg. Insulin partially recovered the antiarrhythmic effect of the blockers. We concluded that diabetes mellitus reduces the antiarrhythmic effects of flecainide, E-4031, and verapamil.
|
['Animals', 'Anti-Arrhythmia Agents', 'Arrhythmias, Cardiac', 'Blood Pressure', 'Diabetes Mellitus', 'Diabetes Mellitus, Experimental', 'Epinephrine', 'Flecainide', 'Halothane', 'Ion Channels', 'Male', 'Piperidines', 'Potassium Channel Blockers', 'Pyridines', 'Rats', 'Rats, Sprague-Dawley', 'Sodium Channel Blockers', 'Verapamil']
| 16,931,659
|
[['B01.050'], ['D27.505.954.411.097'], ['C14.280.067', 'C23.550.073'], ['E01.370.600.875.249', 'G09.330.380.076'], ['C18.452.394.750', 'C19.246'], ['C18.452.394.750.074', 'C19.246.240', 'E05.598.500.374'], ['D02.033.100.291.310', 'D02.092.063.291.310', 'D02.092.211.215.454', 'D02.092.311.461', 'D02.455.426.559.389.657.166.175.461'], ['D03.383.621.270'], ['D02.455.526.340'], ['D12.776.157.530.400', 'D12.776.543.550.450', 'D12.776.543.585.400'], ['D03.383.621'], ['D27.505.519.562.500', 'D27.505.954.411.645'], ['D03.383.725'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.750'], ['D27.505.519.562.750', 'D27.505.954.411.720'], ['D02.092.471.683.953']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Optimization of matrix assisted desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) for the characterization of Bacillus and Brevibacillus species.
|
Over the past few decades there has been an increased interest in using various analytical techniques for detecting and identifying microorganisms. More recently there has been an explosion in the application of matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) for bacterial characterization, and here we optimize this approach in order to generate reproducible MS data from bacteria belonging to the genera Bacillus and Brevibacillus. Unfortunately MALDI-TOF-MS generates large amounts of data and is prone to instrumental drift. To overcome these challenges we have developed a preprocessing pipeline that includes baseline correction, peak alignment followed by peak picking that in combination significantly reduces the dimensionality of the MS spectra and corrects for instrument drift. Following this two different prediction models were used which are based on support vector machines and these generated satisfactory prediction accuracies of approximately 90%.
|
['Bacillus', 'Brevibacillus', 'Species Specificity', 'Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization']
| 25,086,893
|
[['B03.300.390.400.158.218', 'B03.353.500.100.218', 'B03.510.100.100.218', 'B03.510.415.400.158.218', 'B03.510.460.410.158.218'], ['B03.300.390.400.160', 'B03.353.500.117', 'B03.510.100.117', 'B03.510.415.400.179', 'B03.510.460.410.165'], ['G16.824'], ['E05.196.566.755']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Mass spectrometry imaging of small molecules using matrix-enhanced surface-assisted laser desorption/ionization mass spectrometry (ME-SALDI-MS).
|
Surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) uses inorganic particles or porous surfaces as the energy-mediating means to promote desorption and ionization of low-mass analytes of interest. With good stability during laser ablation, SALDI substrates exhibit reduced background in the low-mass region that is often crowded in conventional matrix-assisted laser desorption/ionization (MALDI) due to matrix fragmentation; a benefit renders SALDI-MS attractive in imaging low-mass species. Practical application of SALDI-MS, however, is hindered by its unsatisfied detection sensitivity for most compounds. With aims of improving MS imaging resolution and sensitivity of low-mass species, we describe an experimental protocol using a hybrid ionization method, termed matrix-enhanced SALDI (ME-SALDI), to detect crucial low-mass species with their spatial distribution in mouse brain tissue.
|
['Animals', 'Brain', 'Cerebellum', 'Cerebrum', 'Diagnostic Imaging', 'Mass Spectrometry', 'Mice', 'Microscopy']
| 20,680,595
|
[['B01.050'], ['A08.186.211'], ['A08.186.211.132.810.428.200'], ['A08.186.211.200.885.287'], ['E01.370.350'], ['E05.196.566'], ['B01.050.150.900.649.313.992.635.505.500'], ['E01.370.350.515', 'E05.595', 'H01.671.617.562']]
|
['Organisms [B]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]']
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
A prevalence survey of mental disorders among children in a rural Malaysian village.
|
This is the first epidemiological survey of mental disorders among children in a Malaysian village. The WHO Research Questionnaire for Children (RQC) was used for initial screening followed by a semistructured interview, the Follow-up Interview for Children (FIC) at its second stage. Diagnosis was obtained by using Rutter's multi-axial classification. The prevalence rates obtained for the diagnostic categories were described in 3 age groups, in accordance with the age of attending school in Malaysia. Of 507 children screened aged 1-15, 6.1% were classified as having a mental disorder.
|
['Adolescent', 'Age Factors', 'Child', 'Child, Preschool', 'Cross-Sectional Studies', 'Developing Countries', 'Female', 'Humans', 'Incidence', 'Infant', 'Malaysia', 'Male', 'Mass Screening', 'Mental Disorders', 'Risk Factors', 'Rural Population', 'Sex Factors']
| 8,488,745
|
[['M01.060.057'], ['N05.715.350.075', 'N06.850.490.250'], ['M01.060.406'], ['M01.060.406.448'], ['E05.318.372.500.875', 'N05.715.360.330.500.875', 'N06.850.520.450.500.875'], ['I01.615.500.300'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.308.985.525.375', 'N01.224.935.597.500', 'N06.850.505.400.975.525.375', 'N06.850.520.308.985.525.375'], ['M01.060.703'], ['Z01.252.145.487'], ['E01.370.500', 'E05.318.308.980.438.580', 'N02.421.726.233.443', 'N05.715.360.300.800.438.500', 'N06.850.520.308.980.438.580', 'N06.850.780.500'], ['F03'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['N01.600.725'], ['N05.715.350.675', 'N06.850.490.875']]
|
['Named Groups [M]', 'Health Care [N]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Organisms [B]', 'Geographicals [Z]', 'Psychiatry and Psychology [F]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 1
|
Gonadotropin-releasing hormone induces classical meiotic maturation in subpopulations of atretic preantral follicles.
|
GnRH has been shown to induce premature meiotic maturation in preantral follicles of the immature estrogen-primed hypophysectomized rat. As these animals are free of circulating gonadotropins and contain large numbers of full-grown oocytes in preantral follicles, we have investigated this model to determine its usefulness in studying meiotic maturation. We show that a maximum dose of the agonist D-Trp6,Pro9,Net-LRF (GnRH-a) induces approximately 25% of full grown oocytes to resume meiosis within a 12-h period. This response is dose dependent (ED50 = 0.24 microgram/rat) and specific for GnRH-a. GnRH-a stimulates germinal vesicle breakdown and first polar body formation within 2 and 8 h, respectively. More than 75% of those oocytes that initiate meiotic maturation reach metaphase II by 15 h. This effect of GnRH parallels the time course of physiological meiotic maturation triggered by LH as well as that of oocytes maturing spontaneously in vitro. Oocytes in primordial and primary follicles do not respond to GnRH. The majority of affected follicles are small tertiary follicles (200-400 micron in diameter) and show signs of atresia. This atresia is not caused by GnRH-a and does not, in itself, result in meiotic maturation, but appears to confer susceptibility to GnRH-a-induced meiotic maturation. Our studies indicate that this animal model will be useful to elucidate further the mechanisms and requirements for meiotic maturation. It will also facilitate investigation of the role of atresia in the GnRH response of tertiary follicles and the issue of follicle heterogeneity within these animals.
|
['Animals', 'Diethylstilbestrol', 'Dose-Response Relationship, Drug', 'Female', 'Follicular Atresia', 'Gonadotropin-Releasing Hormone', 'Meiosis', 'Ovarian Follicle', 'Rats', 'Rats, Inbred Strains', 'Time Factors', 'Triptorelin Pamoate']
| 3,896,759
|
[['B01.050'], ['D02.455.426.559.389.150.700.725.500'], ['G07.690.773.875', 'G07.690.936.500'], ['G08.686.290'], ['D06.472.699.327.740.320', 'D12.644.400.400.740.320', 'D12.644.456.460', 'D12.644.548.365.740.320', 'D12.776.631.650.405.740.320'], ['G04.144.220.220.687', 'G05.113.220.687'], ['A05.360.319.114.630.535', 'A06.300.312.497.535'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.050.199.520.760', 'B01.050.150.900.649.313.992.635.505.700.400'], ['G01.910.857'], ['D06.472.699.327.740.320.790', 'D12.644.400.400.740.320.790', 'D12.644.456.460.800', 'D12.644.548.365.740.320.790', 'D12.776.631.650.405.740.320.790']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Relative salience of syllable structure and syllable order in zebra finch song.
|
There is a rich history of behavioral and neurobiological research focused on the 'syntax' of birdsong as a model for human language and complex auditory perception. Zebra finches are one of the most widely studied songbird species in this area of investigation. As they produce song syllables in a fixed sequence, it is reasonable to assume that adult zebra finches are also sensitive to the order of syllables within their song; however, results from electrophysiological and behavioral studies provide somewhat mixed evidence on exactly how sensitive zebra finches are to syllable order as compared, say, to syllable structure. Here, we investigate how well adult zebra finches can discriminate changes in syllable order relative to changes in syllable structure in their natural song motifs. In addition, we identify a possible role for experience in enhancing sensitivity to syllable order. We found that both male and female adult zebra finches are surprisingly poor at discriminating changes to the order of syllables within their species-specific song motifs, but are extraordinarily good at discriminating changes to syllable structure (i.e., reversals) in specific syllables. Direct experience or familiarity with a song, either using the bird's own song (BOS) or the song of a flock mate as the test stimulus, improved both male and female zebra finches' sensitivity to syllable order. However, even with experience, birds remained much more sensitive to structural changes in syllables. These results help to clarify some of the ambiguities from the literature on the discriminability of changes in syllable order in zebra finches, provide potential insight on the ethological significance of zebra finch song features, and suggest new avenues of investigation in using zebra finches as animal models for sequential sound processing.
|
['Animals', 'Auditory Perception', 'Female', 'Finches', 'Male', 'Species Specificity', 'Vocalization, Animal']
| 29,766,379
|
[['B01.050'], ['F02.463.593.071', 'G07.888.125'], ['B01.050.150.900.248.620.750.250'], ['G16.824'], ['F01.145.113.055.800']]
|
['Organisms [B]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Pharmacokinetic properties and in-vivo biological activity of recombinant human erythropoietin encapsulated in red blood cells.
|
The in-vivo survival of 51Cr-labelled murine red blood cells (RBCs) loaded with recombinant human erythropoietin (rhEpo-RBCs) was slightly lower than that of normal RBCs. Intravenous administration to normal mice of the encapsulated rhEpo shows the pharmacokinetic bicompartmental profile typical of the free rhEpo. Distribution and elimination half-life values for the RBC-entrapped rhEpo were no longer than those for the free protein. The area under the curve value was significantly increased for rhEpo-RBCs. Hypertransfused polycythaemic mice were evaluated as an adequate animal model to study the in vivo biological activity of encapsulated rhEpo. rhEpo-RBCs stimulate the erythropoiesis of polycythaemic mice in a linear dose-radio-iron incorporation response relationship. These results suggest that rhEpo-RBCs may behave as an alternative to the administration of free rhEpo in the clinical field.
|
['Animals', 'Cell Division', 'Disease Models, Animal', 'Drug Carriers', 'Drug Delivery Systems', 'Erythrocyte Aging', 'Erythrocytes', 'Erythropoietin', 'Humans', 'Mice', 'Mice, Inbred Strains', 'Polycythemia', 'Recombinant Proteins']
| 9,067,098
|
[['B01.050'], ['G04.144.220', 'G04.161.750.500', 'G05.113', 'G07.345.249.410.750.500'], ['C22.232', 'E05.598.500', 'E05.599.395.080'], ['D26.255.260', 'E02.319.300.380'], ['E02.319.300'], ['G04.043.260', 'G09.188.230'], ['A11.118.290', 'A11.443.240', 'A15.145.229.334'], ['D12.644.276.374.410.240.150', 'D12.776.395.240.150', 'D12.776.467.374.410.240.150', 'D23.529.374.410.240.150'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520', 'B01.050.150.900.649.313.992.635.505.500.400'], ['C15.378.738'], ['D12.776.828']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Hereditary agenesis of ten maxillary posterior teeth: a family history.
|
A family is described, where the mother and her two sons had similar bilateral congenital absences of the following maxillary teeth: the second premolars, the first, second and possibly the third molars, as well as, in the sons, the second primary molars. The size, shape and formation of the remaining teeth were within normal limits. The near relatives of the parents had no history of hypodontia. The family members were otherwise healthy except that the mother and one of the sons had a slight nasality of speech. Moreover, the mother and both sons had adhesive otitis.
|
['Adult', 'Anodontia', 'Bicuspid', 'Child', 'Female', 'Humans', 'Male', 'Maxilla', 'Molar', 'Tooth, Deciduous']
| 3,857,243
|
[['M01.060.116'], ['C07.650.800.100', 'C07.793.700.100', 'C16.131.850.800.100'], ['A14.549.167.860.150'], ['M01.060.406'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A02.835.232.781.324.502.645', 'A14.521.645'], ['A14.549.167.860.525'], ['A14.549.167.860.700']]
|
['Named Groups [M]', 'Diseases [C]', 'Anatomy [A]', 'Organisms [B]']
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
REM sleep deprivation and food intake.
|
The effect of REM-sleep deprivation (REM-SD) on diet preference was studied in rats. REM-SD for a period of 72 hrs produced an increase in day, night and 24 hrs (day plus night) intakes of Carbohydrate Rich diet (CRD) and Total diet (TD). Body weight (BWt) was also increased. The maximum increase in the above parameters were recorded on the 2nd day of REM-SD. During recovery period the intakes of TD fully recovered, but the BWt and consumption of CRD remained high. Intakes of Balanced diet (BD) remained significantly on the lower side when compared to the pre REM-SD mean values. During REM-SD, the rats preferred CRD than BD. The body temperature did not show any change. The increase in TD intake and BWt could be the result of an increase in insulin level and the change appears to be mediated by the activation of hypothalamic feeding centre.
|
['Animals', 'Body Temperature', 'Body Weight', 'Diet', 'Dietary Carbohydrates', 'Dietary Proteins', 'Eating', 'Male', 'Rats', 'Sleep Deprivation', 'Sleep, REM']
| 2,592,037
|
[['B01.050'], ['E01.370.600.875.374', 'G07.110'], ['C23.888.144', 'E01.370.600.115.100.160.120', 'E05.041.124.160.750', 'G07.100.100.160.120', 'G07.345.249.314.120'], ['G07.203.650.240'], ['D09.301', 'G07.203.300.362', 'J02.500.362'], ['D12.776.256', 'G07.203.300.428', 'J02.500.428'], ['G07.203.650.283', 'G10.261.330'], ['B01.050.150.900.649.313.992.635.505.700'], ['C10.886.425.175', 'C23.888.592.796.772', 'F02.830.855.671', 'F03.870.400.099'], ['F02.830.855.796.671', 'G11.561.803.754.671']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Technology, Industry, and Agriculture [J]', 'Psychiatry and Psychology [F]']
| 0
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Effect of zinc sulphate on gelling properties of phosphorylated protein isolate from yellow stripe trevally.
|
Impacts of zinc sulphate (ZnSO4) (0-140 ìmol/kg) on gel properties of yellow stripe trevally surimi added with sodium tripolyphosphate (STPP) (0.25% and 0.5%, w/w) and protein isolate phosphorylated with STPP at 0.25% and 0.5% (w/w) were studied. Gels from surimi added with 60 ìmol ZnSO4/kg in the absence and presence of 0.5% STPP had the increases in breaking force and deformation by 20.9% and 33.3%, and 11.6% and 18.6%, respectively, compared with the control surimi gel (without additives). Gel of protein isolate phosphorylated with 0.5% STPP containing 100 ìmol ZnSO4/kg had the increases in breaking force and deformation by 14.87% and 5.6%, respectively, compared with the gel from non-phosphorylated protein isolate at the same ZnSO4 level, suggesting that the phosphorylated protein isolate was more crosslinked by Zn(2+). The addition of ZnSO4 at the suitable level lowered the expressible moisture content, but increased whiteness of surimi or protein isolate gels (P<0.05). Non-covalent bonds, more likely salt bridge and ionic interactions, played a major role in cross-linking of proteins in both surimi and protein isolate added with ZnSO4, regardless of phosphates incorporated. Microstructure study revealed that a gel having highly interconnected and denser network with smaller voids was formed when protein isolate phosphorylated with 0.5% STPP was added with ZnSO4 at a level of 100 ìmol/kg. Thus, gel with improved properties could be obtained from protein isolate from yellow stripe trevally phosphorylated with STPP in conjunction with addition of ZnSO4 at an appropriate level.
|
['Animals', 'Fish Products', 'Fish Proteins', 'Food Handling', 'Gels', 'Perciformes', 'Phosphorylation', 'Polyphosphates', 'Zinc Sulfate']
| 23,871,033
|
[['B01.050'], ['G07.203.300.600.875.400', 'J02.500.600.875.400'], ['D12.776.325'], ['J01.576.423.200'], ['D20.280.320', 'D26.255.165.320'], ['B01.050.150.900.493.602'], ['G02.111.665', 'G02.607.780', 'G03.796'], ['D01.029.260.700.675.374.775', 'D01.248.497.158.730.650', 'D01.695.625.675.650.775'], ['D01.875.800.800.850.950', 'D01.975.987']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Technology, Industry, and Agriculture [J]', 'Chemicals and Drugs [D]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Preoperative computed tomography-guided localization of ground-glass opacities with metallic clip.
|
Intraoperative localization of ground-glass opacities is difficult because they are not easy to palpate and may be invisible at radioscopy. Therefore, various techniques have been developed to improve intraoperative localization of these lesions, allowing an adequate surgical resection. The aim of this study is to report two cases of preoperative localization of ground-glass opacities through computed tomography-guided placement of a metallic clip inside the lesion and to discuss this new technique in comparison with those previously described.
|
['Adenocarcinoma', 'Aged', 'Antineoplastic Combined Chemotherapy Protocols', 'Diagnosis, Differential', 'Female', 'Humans', 'Image-Guided Biopsy', 'Lung Neoplasms', 'Middle Aged', 'Pneumonectomy', 'Preoperative Care', 'Risk Assessment', 'Sampling Studies', 'Solitary Pulmonary Nodule', 'Surgical Instruments', 'Thoracic Surgery, Video-Assisted', 'Tomography, X-Ray Computed', 'Treatment Outcome']
| 23,992,711
|
[['C04.557.470.200.025'], ['M01.060.116.100'], ['E02.183.750.500', 'E02.319.077.500', 'E02.319.310.037'], ['E01.171'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.225.500.384.100.370', 'E01.370.225.998.054.370', 'E01.370.388.100.370', 'E04.074.370', 'E05.200.500.384.100.370', 'E05.200.998.054.370', 'E05.242.384.100.370'], ['C04.588.894.797.520', 'C08.381.540', 'C08.785.520'], ['M01.060.116.630'], ['E04.620', 'E04.928.600.600'], ['E02.760.795', 'E04.604.750', 'N02.421.585.795'], ['E05.318.740.600.800.715', 'N04.452.871.715', 'N05.715.360.750.625.700.690', 'N06.850.505.715', 'N06.850.520.830.600.800.715'], ['E05.318.372.875', 'N05.715.360.330.875', 'N06.850.520.450.875'], ['C08.381.884'], ['E07.858.700'], ['E01.370.388.250.840.830', 'E01.370.388.250.950.830', 'E04.502.250.840.830', 'E04.502.250.950.830', 'E04.928.752.830'], ['E01.370.350.350.810', 'E01.370.350.600.350.700.810', 'E01.370.350.700.700.810', 'E01.370.350.700.810.810', 'E01.370.350.825.810.810'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Diseases [C]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Health Care [N]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
New Bacterial Phytase through Metagenomic Prospection.
|
Alkaline phytases from uncultured microorganisms, which hydrolyze phytate to less phosphorylated myo-inositols and inorganic phosphate, have great potential as additives in agricultural industry. The development of metagenomics has stemmed from the ineluctable evidence that as-yet-uncultured microorganisms represent the vast majority of organisms in most environments on earth. In this study, a gene encoding a phytase was cloned from red rice crop residues and castor bean cake using a metagenomics strategy. The amino acid identity between this gene and its closest published counterparts is lower than 60%. The phytase was named PhyRC001 and was biochemically characterized. This recombinant protein showed activity on sodium phytate, indicating that PhyRC001 is a hydrolase enzyme. The enzymatic activity was optimal at a pH of 7.0 and at a temperature of 35 °C. â-propeller phytases possess great potential as feed additives because they are the only type of phytase with high activity at neutral pH. Therefore, to explore and exploit the underlying mechanism for â-propeller phytase functions could be of great benefit to biotechnology.
|
['6-Phytase', 'Amino Acid Sequence', 'Bacteria', 'Environment', 'Enzyme Stability', 'Gene Library', 'Genes, Bacterial', 'Hydrogen-Ion Concentration', 'Ions', 'Metagenomics', 'Metals', 'Models, Molecular', 'Phylogeny', 'Recombinant Proteins', 'Sequence Analysis, DNA', 'Structural Homology, Protein', 'Substrate Specificity', 'Temperature']
| 29,462,992
|
[['D08.811.277.352.650.700'], ['G02.111.570.060', 'L01.453.245.667.060'], ['B03'], ['G16.500.275', 'N06.230'], ['E05.916.360', 'G02.111.700.500'], ['G05.360.325'], ['G05.360.340.024.340.364.249', 'G05.360.340.358.024.249', 'G05.360.340.358.207.249'], ['G02.300'], ['D01.248.497'], ['H01.158.273.343.350.261'], ['D01.552'], ['E05.599.595'], ['G05.697', 'G16.075.605', 'L01.100.697'], ['D12.776.828'], ['E05.393.760.700'], ['G02.111.570.820.709.805', 'G02.111.810.200.820', 'G05.820'], ['G02.111.835'], ['G01.906.595', 'G16.500.275.063.725.710', 'G16.500.750.775.710', 'N06.230.150.450', 'N06.230.300.100.725.710']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Organisms [B]', 'Health Care [N]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 0
|
Radiation response of mouse lymphoid and myeloid cell lines. Part I. Sensitivity to killing by ionizing radiation, rate of loss of viability, and cell type of origin.
|
The sensitivity of 10 mouse lymphoid or myeloid cell lines to gamma-ray- and DNA-associated 125I-decay-induced clonogenic cell killing have been compared with their rate of loss of viability (membrane integrity) and with their putative cell type of origin. The pseudodiploid haematopoietic cell lines showed D0 values for 125I-induced DNA double-strand breakage (dsb) that ranged from 7.7 +/- 0.7 to 40.8 +/- 2.8 decays. These lines generally appeared to be more sensitive to killing by radiation-induced DNA dsb than are fibroblast-like cell lines. The increased sensitivity of haematopoietic cell lines to killing by DNA dsb may be related to their mode of death (apoptosis versus necrosis). Mode of cell death may thus be an important factor in determining the 'inherent radiosensitivity' of normal cells/tissues. Haematopoietic cell lines that undergo rapid interphase apoptotic death showed extreme sensitivity to DNA dsb. The latter cell lines were found to have derived from immature lymphoid cells, and it is speculated that their high radiosensitivity might reflect the action of a mechanism that normally eliminates cells containing illegitimate V(D)J recombinase-induced DNA dsb.
|
['Animals', 'Bone Marrow', 'Cell Survival', 'DNA Damage', 'Gamma Rays', 'Gene Rearrangement, T-Lymphocyte', 'Immunophenotyping', 'Lymphocytes', 'Mice', 'Mice, Inbred BALB C', 'Mice, Inbred DBA', 'Receptors, Antigen, T-Cell, alpha-beta']
| 7,907,118
|
[['B01.050'], ['A15.382.216'], ['G04.346'], ['G05.200'], ['G01.358.500.505.300', 'G01.750.250.300', 'G01.750.750.400'], ['G05.344.801', 'G12.500.287'], ['E01.370.225.812.447', 'E05.200.812.447', 'E05.478.594.450'], ['A11.118.637.555.567', 'A15.145.229.637.555.567', 'A15.382.490.555.567'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.338', 'B01.050.150.900.649.313.992.635.505.500.400.338'], ['B01.050.050.199.520.520.500', 'B01.050.150.900.649.313.992.635.505.500.400.500'], ['D12.776.543.750.705.816.824.825']]
|
['Organisms [B]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Cytostatic and cytotoxic properties of the marine product bistratene A and analysis of the role of protein kinase C in its mode of action.
|
Bistratene A is a polyether which was isolated from the marine ascidian Lissoclinum bistratum Sluiter. The hypothesis has been tested that the cytostatic effect of bistratene A is mediated by modulation of protein kinase C (PKC). Human-derived A549 lung and MCF-7 breast adenocarcinoma cells are extremely sensitive to growth inhibition induced by activators of PKC. Therefore, the effect of bistratene A on these cell lines was compared with that of the known PKC activator 12-O-tetradecanoylphorbol-13-acetate (TPA). The ability of bistratene A to modulate PKC activity in cellular cytosol was assessed to determine the involvement of PKC in the induction of cytostasis. Bistratene A inhibited the growth of both cell lines and initial seeding density determined its cytostatic potency. IC50 values were between 1.0 and 2.9 nM. Bistratene A also had a profound effect on the colony forming ability of A549 cells, preventing clonal growth at 5 nM. Using the incorporation of [3H]thymidine into cells to assess DNA synthetic activity and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay to define cytotoxicity, the compound was found to have both cytostatic and cytotoxic properties. Bistratene A decomposed by 50% after only 2.8 hr in cell culture medium. TPA induced rapid motility and the formation of a network of branched colonies in both cell lines grown on Matrigel, whereas bistratene A did not cause the same effect. Cell cytosol was analysed for phorbol ester binding sites after treatment with bistratene A or TPA. Incubation with TPA (10 nM) caused a reduction in binding sites to 57% of binding in control cells after 30 min and to 35% after 24 hr. Bistratene A did not cause a significant change in binding sites. Assays of PKC activity in cellular cytosol revealed that bistratene A was unable to activate or inhibit the enzyme at concentrations of up to 10 microM. The results suggest that bistratene A is an exquisitely potent cytostatic agent in the two cell lines studied, but modulation of PKC is not involved in the mode of action by which it elicits this effect.
|
['Acetamides', 'Cell Division', 'Cell Line', 'Cytosol', 'Enzyme Activation', 'Ethers, Cyclic', 'Humans', 'Marine Toxins', 'Protein Kinase C', 'Pyrans', 'Signal Transduction', 'Spiro Compounds', 'Structure-Activity Relationship', 'Tetradecanoylphorbol Acetate', 'Tumor Cells, Cultured']
| 8,494,533
|
[['D02.065.064', 'D02.241.081.018.110'], ['G04.144.220', 'G04.161.750.500', 'G05.113', 'G07.345.249.410.750.500'], ['A11.251.210'], ['A11.284.430.214.200', 'A11.284.430.429.200', 'A11.284.835.450.200'], ['G02.111.263', 'G03.328'], ['D02.355.291', 'D04.345.241'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D23.946.580'], ['D08.811.913.696.620.682.700.725'], ['D03.383.663'], ['G02.111.820', 'G04.835'], ['D02.455.426.779', 'D04.711'], ['G02.111.830', 'G07.690.773.997'], ['D02.455.849.291.500.510.850'], ['A11.251.860']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Organisms [B]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
NF-êB-regulated microRNA-574-5p underlies synaptic and cognitive impairment in response to atmospheric PM2.5
|
BACKGROUND: PM2.5 (particulate matter ? 2.5 ìm) is one of the leading environmental risk factors for the global burden of disease. Whereas increasing evidence has linked the adverse roles of PM2.5 with cardiovascular and respiratory diseases, limited but growing emerging evidence suggests that PM2.5 exposure can affect the nervous system, causing neuroinflammation, synaptic dysfunction and cognitive deterioration. However, the molecular mechanisms underlying the synaptic and cognitive deficits elicited by PM2.5 exposure are largely unknown.METHODS: C57BL/6 mice received oropharyngeal aspiration of PM2.5 (1 and 5 mg/kg bw) every other day for 4 weeks. The mice were also stereotaxically injected with â-site amyloid precursor protein cleaving enzyme 1 (â-secretase, BACE1) shRNA or LV-miR-574-5p lentiviral constructs in the absence or presence of PM2.5 aspiration at 5 mg/kg bw every other day for 4 weeks. Spatial learning and memory were assessed with the Morris water maze test, and synaptic function integrity was evaluated with electrophysiological recordings of long-term potentiation (LTP) and immunoblot analyses of glutamate receptor subunit expression. The expression of á-secretase (ADAM10), BACE1, and ã-secretase (nicastrin) and the synthesis and accumulation of amyloid â (Aâ) were measured by immunoblot and enzyme-linked immunosorbent assay (ELISA). MicroRNA (miRNA) expression was screened with a microRNA microarray analysis and confirmed by real-time quantitative reverse transcription PCR (qRT-PCR) analysis. Dual-luciferase reporter gene and chromatin immunoprecipitation (ChIP) analyses were used to detect the binding of miR-574-5p in the 3'UTR of BACE1 and NF-êB p65 in the promoter of miR-574-5p, respectively.RESULTS: PM2.5 aspiration caused neuroinflammation and deteriorated synaptic function integrity and spatial learning and memory, and the effects were associated with the induction of BACE1. The action was mediated by NF-êB p65-regulated downregulation of miR-574-5p, which targets BACE1. Overexpression of miR-574-5p in the hippocampal region decreased BACE1 expression, restored synaptic function, and improved spatial memory and learning following PM2.5 exposure.CONCLUSIONS: Taken together, our findings reveal a novel molecular mechanism underlying impaired synaptic and cognitive function following exposure to PM2.5, suggesting that miR-574-5p is a potential intervention target for the prevention and treatment of PM2.5-induced neurological disorders.
|
['Air Pollutants', 'Animals', 'Cognitive Dysfunction', 'Gene Expression Regulation', 'Hippocampus', 'Inhalation Exposure', 'Maze Learning', 'Mice, Inbred C57BL', 'MicroRNAs', 'NF-kappa B', 'Particle Size', 'Particulate Matter', 'Spatial Memory', 'Synaptic Potentials']
| 28,851,397
|
[['D27.888.284.101'], ['B01.050'], ['F03.615.250.700'], ['G05.308'], ['A08.186.211.180.405', 'A08.186.211.200.885.287.500.345'], ['N06.850.460.350.112'], ['F02.463.425.874.500'], ['B01.050.050.199.520.520.420', 'B01.050.150.900.649.313.992.635.505.500.400.420'], ['D13.150.650.319', 'D13.444.735.150.319', 'D13.444.735.790.552.500'], ['D05.500.672', 'D12.776.260.600', 'D12.776.660.600', 'D12.776.930.600'], ['G02.712'], ['D20.633'], ['F02.463.425.540.886'], ['G04.580.887', 'G07.265.675.887', 'G07.265.880.750', 'G11.561.570.918', 'G11.561.830.750']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Health Care [N]']
| 1
| 1
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Unchanged unilateral hearing loss and ipsilateral growth of an acoustic neuroma from 1 to 4 cm.
|
Progressive sensorineural hearing loss is the most important early symptom of a cerebellopontine angle process. A case report is presented of a 42-year-old woman who was referred to our department in 1979. Oil cisternography showed non filling of the left internal acoustic canal. Audiometry was planned as the method of control, but she did not return until nine-years later. In 1988, an acoustic neurinoma of 4 cm diameter was found in the left CPA. Pure tone audiometry and speech audiometry showed that during the nine-year interval, her 60 dB flat sensorineural hearing loss and speech perception thresholds had remained almost unchanged. A follow-up with only tone and speech audiometry can lead to a false negative diagnosis in some of these cases. Calculation of the growth in tumour volume over nine years in this patient showed a tumour volume doubling time of about 15 months.
|
['Adult', 'Audiometry, Pure-Tone', 'Audiometry, Speech', 'Cerebellopontine Angle', 'Female', 'Follow-Up Studies', 'Hearing Loss, Sensorineural', 'Humans', 'Neuroma, Acoustic', 'Tomography, X-Ray Computed']
| 8,509,702
|
[['M01.060.116'], ['E01.370.382.375.060.055'], ['E01.370.382.375.060.060'], ['A08.186.211.132.810.428.200.462'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['C09.218.458.341.887', 'C10.597.751.418.341.887', 'C23.888.592.763.393.341.887'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C04.557.465.625.650.595.610', 'C04.557.580.600.610.595.610', 'C04.557.580.625.650.595.610', 'C04.588.614.300.015', 'C04.588.614.596.240.015', 'C09.218.807.800.675', 'C09.647.675', 'C10.292.225.750', 'C10.292.910.600'], ['E01.370.350.350.810', 'E01.370.350.600.350.700.810', 'E01.370.350.700.700.810', 'E01.370.350.700.810.810', 'E01.370.350.825.810.810']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Health Care [N]', 'Diseases [C]', 'Organisms [B]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
TWIST1 is a molecular marker for a poor prognosis in oral cancer and represents a potential therapeutic target.
|
BACKGROUND: Locoregional recurrence and distant metastases are ominous events in patients with advanced oral squamous cell carcinoma (OSCC). The objective of this study was to identify functional biomarkers that are predictive of OSCC progression to metastasis.METHODS: The expression profile of a network of epithelial-mesenchymal transition (EMT) genes was investigated in a large cohort of patients with progressive OSCC using a complimentary DNA microarray platform coupled to quantitative reverse transcriptase-polymerase chain reaction and immunohistochemical analyses. Therapeutic potential was investigated in vitro and in vivo using an orthotopic mouse model of metastatic OSCC growing in the tongue microenvironment.RESULTS: Among deregulated EMT genes, the Twist-related protein 1 (TWIST1) transcription factor and several of its regulated genes were significantly overexpressed across advanced stages of OSCC. This result was corroborated by the clinical observation that Twist1 up-regulation predicted the occurrence of lymph node and lung metastases as well as poor patient survival. In support of Twist1 as a driver of OSCC progression, the up-regulation of Twist1 was observed in cells isolated from patients with metastatic OSCC. The inhibition of Twist1 in these metastatic cells induced a potent inhibition of cell invasiveness in vitro as well as progression in vivo.CONCLUSIONS: The current results provide evidence for the prognostic value and therapeutic potential of a network of Twist genes in patients with advanced OSCC.
|
['Animals', 'Carcinoma, Squamous Cell', 'Cells, Cultured', 'Epithelial-Mesenchymal Transition', 'Female', 'Gene Expression Profiling', 'Humans', 'Male', 'Mice', 'Mouth Neoplasms', 'Nuclear Proteins', 'Prognosis', 'Twist-Related Protein 1']
| 24,150,986
|
[['B01.050'], ['C04.557.470.200.400', 'C04.557.470.700.400'], ['A11.251'], ['G04.356.500'], ['E05.393.332'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['B01.050.150.900.649.313.992.635.505.500'], ['C04.588.443.591', 'C07.465.530'], ['D12.776.660'], ['E01.789'], ['D12.776.260.103.906.250', 'D12.776.930.125.906.250']]
|
['Organisms [B]', 'Diseases [C]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Immunocytological studies of Epstein-Barr viral antigen and antibody in rheumatoid synovial fluids.
|
Evidence for intra-articular immunity to Epstein-Barr virus (EBV) was sought in synovial fluids (SF) and SF phagocytes derived from patients with and without rheumatoid arthritis (RA). Antibody titres to EBV were not significantly different in SF of 32 RA and 25 non-RA patients. Whereas the majority of RA patients (69%) showed Ig-containing complexes (IC) in SF phagocytes, fluorescent antibody staining of these by anti-EBV serum was negative except with 6 RA patients. Extended analysis of the SF phagocytes of the latter, however, showed no EBV specificity in their IC, suggesting that these represented non-specific "pseudo-IC'. These studies do not support a role for EBV-containing IC in the propagation of rheumatoid synovitis and demonstrate that not all immunofluorescent inclusions in RA phagocytes (ragocytes) represent immune complexes.
|
['Antibodies, Viral', 'Antigens, Viral', 'Arthritis, Rheumatoid', 'Complement C3', 'Female', 'Herpesvirus 4, Human', 'Humans', 'Immunoglobulins', 'Male', 'Phagocytes', 'Synovial Fluid']
| 6,310,723
|
[['D12.776.124.486.485.114.254', 'D12.776.124.790.651.114.254', 'D12.776.377.715.548.114.254'], ['D23.050.327'], ['C05.550.114.154', 'C05.799.114', 'C17.300.775.099', 'C20.111.199'], ['D12.776.124.050.140', 'D12.776.124.486.274.250'], ['B04.280.210.400.500.450', 'B04.280.382.400.500.400', 'B04.613.204.500.500.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.776.124.486.485', 'D12.776.124.790.651', 'D12.776.377.715.548'], ['A11.733', 'A15.382.680'], ['A02.835.583.443.800.800', 'A12.207.270.847']]
|
['Chemicals and Drugs [D]', 'Diseases [C]', 'Organisms [B]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
PON1 55 and 192 gene polymorphisms in type 2 diabetes mellitus patients in a Turkish population.
|
Diabetes mellitus is a multifactorial metabolic disease, caused by the complete or relative absence of insulin hormone, which results in the deterioration of carbohydrate, protein, and lipid metabolism. The PON1 55 and 192 polymorphisms have been reported to be associated with type 2 diabetes and its complications. In this study, the involvement of the PON1 55 and 192 polymorphisms and paraoxonase enzyme activity in diabetic complications was assessed. The MM and QQ genotypes were the most frequent in complications of type 2 diabetes in both of the polymorphisms. PON enzyme activity was lower in the type 2 diabetes group with respect to the control group. Regarding both genotypes and enzyme activity, correlations were found between the PON1 55 and 192 genotypes and diabetic complications. This study thus helps to outline a genotype-phenotype relation for the PON1 gene in a Turkish population.
|
['Amino Acid Substitution', 'Aryldialkylphosphatase', 'Body Mass Index', 'Case-Control Studies', 'Coronary Artery Disease', 'Diabetes Mellitus, Type 2', 'Diabetic Angiopathies', 'Gene Frequency', 'Genetic Association Studies', 'Genetics, Population', 'Genotype', 'Humans', 'Middle Aged', 'Phenotype', 'Polymorphism, Genetic', 'Turkey']
| 20,820,904
|
[['E05.393.420.601.035', 'G05.558.109'], ['D08.811.277.352.660.500'], ['E01.370.600.115.100.125', 'E05.041.124.125', 'G07.100.100.125', 'N06.850.505.200.100.175'], ['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['C14.280.647.250.260', 'C14.907.137.126.339', 'C14.907.585.250.260'], ['C18.452.394.750.149', 'C19.246.300'], ['C14.907.320', 'C19.246.099.500'], ['G05.330'], ['E05.393.385'], ['H01.158.273.343.335'], ['G05.380'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['G05.695'], ['G05.365.795'], ['Z01.252.245.500.850']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Health Care [N]', 'Diseases [C]', 'Disciplines and Occupations [H]', 'Organisms [B]', 'Named Groups [M]', 'Geographicals [Z]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 1
| 1
| 1
|
[Comparative in vitro research on 2 new antacid combinations--Ulsan and Ulsan D].
|
Two new antacid combinations--Ulsan and Ulsan D with high acid-neutralizing properties, produced by original Bulgarian techniques are presented. They exceed in acid-binding and acid-reducing properties the drugs now in use: Antiacid, Neutracid, Almagel, Alcid V, Maaloxan, Gelusil-Lac.
|
['Aluminum', 'Antacids', 'Buffers', 'Drug Combinations', 'Drug Evaluation, Preclinical', 'Drug Interactions', 'Gastric Acid', 'Hydrogen-Ion Concentration', 'In Vitro Techniques', 'Magnesium', 'Plant Extracts', 'Tablets']
| 3,433,734
|
[['D01.268.557.050', 'D01.552.547.050'], ['D27.505.519.170', 'D27.505.954.483.080'], ['D27.720.470.280'], ['D26.310'], ['E05.290.750', 'E05.337.550'], ['G07.690.773.968'], ['A12.200.307.603'], ['G02.300'], ['E05.481'], ['D01.268.552.437', 'D01.268.557.500', 'D01.552.547.500'], ['D20.215.784.500', 'D26.667'], ['D26.255.830']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Anatomy [A]']
| 1
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Potential differences in kidney allograft outcomes between ethnicities when converting to sirolimus base immunosuppression.
|
OBJECTIVE: The aim of this study was to determine whether ethnicity impacts graft outcomes in kidney transplant patients converted to sirolimus (SRL) and maintained on either calcineurin inhibitors (CI) or mycophenolate mofetil (MMF) with steroids.METHODS: This study analyzed kidney transplants converted to SRL and transplanted between July 1991 and April 2007. Patients were divided into 4 groups: group 1: African-Americans converted to SRL + CI; group 2: non-African-Americans converted to SRL + CI; group 3: African-Americans converted to SRL + MMF; group 4: non-African-Americans converted to SRL + MMF.RESULTS: A total of 242 patients was included. Demographics, baseline immunosuppression, and reason for SRL conversion were similar among groups. Patients converted to SRL + CI regimens had significantly higher rates of acute rejection before SRL conversion, but equal rates after conversion. Development of proteinuria was similar across groups. African-American patients converted to SRL + MMF tended to have poorer outcomes compared with African-American patients converted to SRL + CI. Non-African-American patients converted to SRL + MMF tended to have better graft outcomes compared with non-African-American patients converted to SRL + CI.CONCLUSIONS: African-Americans converted to SRL may benefit from continued CI, whereas non-African-Americans converted to SRL seem to have better outcomes with MMF. Further prospective studies are warranted to confirm these findings.
|
['Adolescent', 'Adult', 'African Continental Ancestry Group', 'Continental Population Groups', 'Drug Therapy, Combination', 'Ethnic Groups', 'European Continental Ancestry Group', 'Female', 'Graft Rejection', 'Graft Survival', 'Half-Life', 'Humans', 'Immunosuppressive Agents', 'Kidney Transplantation', 'Living Donors', 'Male', 'Middle Aged', 'Mycophenolic Acid', 'Retrospective Studies', 'Sirolimus', 'Transplantation, Homologous']
| 20,005,354
|
[['M01.060.057'], ['M01.060.116'], ['M01.686.508.100'], ['M01.686.508'], ['E02.319.310'], ['M01.686.754', 'N01.224.317'], ['M01.686.508.400'], ['G12.875.545.328'], ['G12.875.545.340'], ['G01.910.405'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D27.505.696.477.656'], ['E02.870.500', 'E04.936.450.485', 'E04.950.774.400'], ['M01.898.656'], ['M01.060.116.630'], ['D02.241.081.193.678', 'D10.251.618'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['D02.540.505.760'], ['E04.936.864']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Chemicals and Drugs [D]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
A novel "reactomics" approach for cancer diagnostics.
|
Non-invasive detection and monitoring of lethal diseases, such as cancer, are considered as effective factors in treatment and survival. We describe a new disease diagnostic approach, denoted "reactomics", based upon reactions between blood sera and an array of vesicles comprising different lipids and polydiacetylene (PDA), a chromatic polymer. We show that reactions between sera and such a lipid/PDA vesicle array produce chromatic patterns which depend both upon the sera composition as well as the specific lipid constituents within the vesicles. The chromatic patterns were processed through machine-learning algorithms, and the bioinformatics analysis could distinguish both between cancer-bearing and healthy patients, respectively, as well between two types of cancers. Size-separation and enzymatic digestion experiments indicate that lipoproteins are the primary components in sera which react with the chromatic biomimetic vesicles. This colorimetric reactomics concept is highly generic, robust, and does not require a priori knowledge upon specific disease markers in sera. Therefore, it could be employed as complementary or alternative approach for disease diagnostics.
|
['Humans', 'Neoplasms', 'Spectrometry, Fluorescence']
| 22,778,601
|
[['B01.050.150.900.649.313.988.400.112.400.400'], ['C04'], ['E05.196.712.516.600.676', 'E05.196.867.726']]
|
['Organisms [B]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Methionine kinetics in adult men: effects of dietary betaine on L-[2H3-methyl-1-13C]methionine.
|
The effects of a daily 3-g supplement of betaine on kinetic aspects of L-[2H3-methyl-1-13C]methionine (MET) metabolism in healthy young adult men were explored. Four groups of four subjects each were given a control diet, based on an L-amino acid mixture supplying 29.5 and 21.9 mg.kg-1.d-1 of L-methionine and L-cystine for 4 d before the tracer study, conducted on day 5 during the fed state. Two groups received the control diet and two groups received the betaine supplement. Tracer was given intravenously (iv) or orally. The transmethylation rate of MET (TM), homocysteine remethylation (RM), and oxidation of methionine were estimated from plasma methionine labeling and 13C enrichment of expired air. RM tended to increase (P = 0.14) but the TM and methionine oxidation were significantly (P less than 0.05) higher after betaine supplementation when estimated with the oral tracer. No differences were detected with the intravenous tracer. Methionine concentration in plasma obtained from blood taken from subjects in the fed state was higher (P less than 0.01) with betaine supplementation. These results suggest that excess methyl-group intake may increase the dietary requirement for methionine.
|
['Adult', 'Betaine', 'Carbon Isotopes', 'Cystine', 'Deuterium', 'Diet', 'Humans', 'Kinetics', 'Male', 'Methionine', 'Methylation', 'Nutritional Requirements']
| 1,858,702
|
[['M01.060.116'], ['D02.092.877.883.077', 'D02.675.276.125'], ['D01.268.150.075', 'D01.496.123'], ['D01.248.497.158.874.390.369', 'D01.875.350.850.150.369', 'D02.886.030.230.369', 'D02.886.520.150.087', 'D12.125.095.369', 'D12.125.119.369', 'D12.125.166.230.369'], ['D01.268.406.500', 'D01.362.340.500', 'D01.496.289'], ['G07.203.650.240'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G01.374.661', 'G02.111.490'], ['D02.886.030.676', 'D12.125.142.557', 'D12.125.154.549', 'D12.125.166.676'], ['G02.111.035.538', 'G02.607.094.538', 'G03.059.538'], ['G07.203.650.620']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Whole-Genome Sequencing in Microbial Forensic Analysis of Gamma-Irradiated Microbial Materials.
|
Effective microbial forensic analysis of materials used in a potential biological attack requires robust methods of morphological and genetic characterization of the attack materials in order to enable the attribution of the materials to potential sources and to exclude other potential sources. The genetic homogeneity and potential intersample variability of many of the category A to C bioterrorism agents offer a particular challenge to the generation of attributive signatures, potentially requiring whole-genome or proteomic approaches to be utilized. Currently, irradiation of mail is standard practice at several government facilities judged to be at particularly high risk. Thus, initial forensic signatures would need to be recovered from inactivated (nonviable) material. In the study described in this report, we determined the effects of high-dose gamma irradiation on forensic markers of bacterial biothreat agent surrogate organisms with a particular emphasis on the suitability of genomic DNA (gDNA) recovered from such sources as a template for whole-genome analysis. While irradiation of spores and vegetative cells affected the retention of Gram and spore stains and sheared gDNA into small fragments, we found that irradiated material could be utilized to generate accurate whole-genome sequence data on the Illumina and Roche 454 sequencing platforms.
|
['Bacteria', 'Biological Warfare Agents', 'Forensic Sciences', 'Gamma Rays', 'Genome, Bacterial', 'Sequence Analysis, DNA']
| 26,567,301
|
[['B03'], ['D20.215.226', 'J01.637.870.900.100'], ['I01.198.780'], ['G01.358.500.505.300', 'G01.750.250.300', 'G01.750.750.400'], ['G05.360.340.358.207'], ['E05.393.760.700']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Technology, Industry, and Agriculture [J]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
|
Ultrafine Fe3
|
A pyrolytic method is described for preparation of ultrafine Fe3C nanoparticles incorporated into N-doped graphitic carbon nanosheets (Fe3C@NGCSs). Iron phthalocyanine and graphitic carbon nitride (g-C3N4) are used as starting materials. The hybrid nanocomposite was placed on a glassy carbon electrode (GCE) and then applied to simultaneous determination of ascorbic acid (AA), dopamine (DA), uric acid (UA) and xanthine (XA). Figures of merits are as follows: for AA, the linear response range covers the 54.0-5491.0 ìM range, the lower detection limit is 16.7 ìM, and the best working voltage (vs. the saturated calomel electrode (SCE)) is 0.05 V. The respective data for DA are 1.2-120.8 ìM, 0.34 ìM and 0.19 V (vs. SCE). For UA, the respective data are 4.8-263.0 ìM, 1.4 ìM and 0.32 V (vs. SCE), and for XA the data are 4.8-361.0 ìM, 1.5 ìM and 0.71 V (vs. SCE). The method was successfully applied to their simultaneous determination in spiked serum samples. Graphical abstract Ultrafine Fe3C nanoparticles embedded in N-doped graphitic carbon sheets for simultaneous determination of ascorbic acid, dopamine, uric acid and xanthine.
|
['Ascorbic Acid', 'Biosensing Techniques', 'Carbon', 'Carbon Compounds, Inorganic', 'Dopamine', 'Electrochemical Techniques', 'Electrodes', 'Humans', 'Iron Compounds', 'Nanoparticles', 'Particle Size', 'Surface Properties', 'Uric Acid', 'Xanthine']
| 31,471,825
|
[['D02.241.081.844.107', 'D02.241.511.902.107', 'D09.811.100'], ['E05.601.043'], ['D01.268.150'], ['D01.200'], ['D02.092.211.215.406', 'D02.092.311.342', 'D02.455.426.559.389.657.166.175.342'], ['E05.301'], ['E07.305.250'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D01.490'], ['J01.637.512.600'], ['G02.712'], ['G02.860'], ['D03.132.960.877', 'D03.633.100.759.758.824.877'], ['D03.132.960.938', 'D03.633.100.759.758.824.938']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Technology, Industry, and Agriculture [J]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Increased transglutaminase activity was associated with IL-6 release in cultured human gingival fibroblasts exposed to dental cast alloys.
|
Molecular mechanisms underlying gingival and periodontal inflammation caused by dental alloys are still poorly understood. Recently, it has been demonstrated that tissue transglutaminase can be involved in inflammatory cell response. The aim of this study was to evaluate effects of exposure to orthodontic materials on transglutaminase in cultured human gingival fibroblasts. The incubation with Ni-Ti heat-activated (T3) or Ni-Ti super-elastic (T4), and with Ni-Cr-Co (T2) alloys produced respectively 2.5-fold and 8-fold increases in IL-6 release compared with control cultures. Transglutaminase activity was significantly increased in cells exposed to T3 and T4 alloys (about 170% of control; p < 0.05), where it was mainly localized close to inner part of cell membrane. The exposure to T3 and T4 specimens significantly up-regulated also tTG expression compared with control cultures. These data first show an association between IL-6 release and tissue transglutaminase increases, suggesting that TGase-mediated reactions may play a major role in periodontal inflammation.
|
['Cell Line', 'Dental Alloys', 'Enzyme Activation', 'Fibroblasts', 'Gene Expression Regulation, Enzymologic', 'Gingiva', 'Gingivitis', 'Humans', 'Interleukin-6', 'Transglutaminases', 'Up-Regulation']
| 16,583,305
|
[['A11.251.210'], ['D25.339.208', 'J01.637.051.339.208'], ['G02.111.263', 'G03.328'], ['A11.329.228'], ['G05.308.320'], ['A14.549.167.646.480'], ['C01.408', 'C07.465.714.258.480'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.644.276.374.465.224', 'D12.776.467.374.465.202', 'D23.529.374.465.224'], ['D08.811.913.050.200.800'], ['G02.111.905', 'G05.308.850', 'G07.690.773.998']]
|
['Anatomy [A]', 'Chemicals and Drugs [D]', 'Technology, Industry, and Agriculture [J]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Organisms [B]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Effects of NO-donors, SIN-1 and GEA 3175 on prostacyclin and cGMP synthesis in cultured rat endothelial cells.
|
The aim of the present study was to investigate, whether nitric oxide (NO) modifies prostacyclin synthesis in endothelial cells. Two different NO-donors: SIN-1 (3-morpholino sydnonimine) and GEA 3175 (4-aryl-substituted oxatriazol derivative), and the NO-synthesis inhibitor; L-NAME were used. Endothelial cells were incubated with the tested compounds with or without Ca ionophore A23187 stimulation. SIN-1 (> 33 microM) and GEA 3175 (> 1 microM) increased the endothelial cGMP levels independently of A23187 stimulation. SIN-1 did not influence prostacyclin synthesis. GEA 3175 (> 33 microM) increased prostacyclin synthesis up to 2-fold, when incubated without A23187. GEA 3175 with A23187 induced about 30% inhibition in prostacyclin synthesis. L-NAME decreased unstimulated prostacyclin synthesis and this inhibition was reversed by GEA 3175. Obviously NO is able to modulate prostacyclin synthesis, however, much higher concentrations are needed than those to increase cGMP synthesis.
|
['Animals', 'Arginine', 'Calcimycin', 'Cells, Cultured', 'Cyclic GMP', 'Endothelium, Vascular', 'Epoprostenol', 'Female', 'Molsidomine', 'NG-Nitroarginine Methyl Ester', 'Nitric Oxide', 'Rats', 'Rats, Wistar', 'Triazoles']
| 7,717,180
|
[['B01.050'], ['D12.125.068.050', 'D12.125.095.104', 'D12.125.142.087'], ['D03.633.100.221.173'], ['A11.251'], ['D03.633.100.759.646.454.160', 'D13.695.462.275', 'D13.695.667.454.160', 'D13.695.827.426.160'], ['A07.015.700.500', 'A10.272.491.355'], ['D10.251.355.255.550.550.500', 'D23.469.050.175.725.550.500'], ['D03.383.129.462.580.693.450', 'D03.383.533.640.362'], ['D12.125.068.050.525', 'D12.125.095.104.525'], ['D01.339.387', 'D01.625.550.500', 'D01.625.700.500', 'D01.650.550.587.600'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.900'], ['D03.383.129.799']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Spontaneous mentalizing captures variability in the cortical thickness of social brain regions.
|
Theory of mind (ToM)--or thinking about the mental states of others--is a cornerstone of successful everyday social interaction. However, the brain bases of ToM are most frequently measured via explicit laboratory tasks that pose direct questions about mental states (e.g. "In this story, what does Steve think Julia believes?"). Neuroanatomical measures may provide a way to explore the brain bases of individual differences in more naturalistic everyday mentalizing. In the current study, we examined the relation between cortical thickness and spontaneous ToM using the novel Spontaneous Theory of Mind Protocol (STOMP), which measures participants' spontaneous descriptions of the beliefs, emotions and goals of characters in naturalistic videos. We administered standard ToM tasks and the STOMP to young adults (aged 18-26 years) and collected structural magnetic resonance imaging data from a subset of these participants. The STOMP produced robust individual variability and was correlated with performance on traditional ToM tasks. Further, unlike the traditional ToM tasks, STOMP performance was related to cortical thickness for a set of brain regions that have been functionally linked to ToM processing. These findings offer novel insight into the brain bases of variability in naturalistic mentalizing performance, with implications for both typical and atypical populations.
|
['Adolescent', 'Adult', 'Autistic Disorder', 'Brain Mapping', 'Cerebral Cortex', 'Culture', 'Emotions', 'Female', 'Goals', 'Humans', 'Individuality', 'Interpersonal Relations', 'Magnetic Resonance Imaging', 'Male', 'Photic Stimulation', 'Psychomotor Performance', 'Social Behavior', 'Theory of Mind', 'Young Adult']
| 24,847,726
|
[['M01.060.057'], ['M01.060.116'], ['F03.625.164.113.500'], ['E01.370.350.578.875.500', 'E01.370.376.537.625.500', 'E05.629.875.500'], ['A08.186.211.200.885.287.500'], ['I01.076.201.450', 'I01.880.853.100'], ['F01.470'], ['F01.658.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F01.752.488'], ['F01.829.401'], ['E01.370.350.825.500'], ['E05.723.729'], ['F02.808', 'G11.427.700', 'G11.561.660'], ['F01.145.813'], ['F02.463.689', 'F02.739.897'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Psychiatry and Psychology [F]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Organisms [B]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 0
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
|
Characterization of lipid-rich aortic plaques by intravascular photoacoustic tomography: ex vivo and in vivo validation in a rabbit atherosclerosis model with histologic correlation.
|
BACKGROUND: Histologic studies have demonstrated that lipid content and its spatial distribution is related to plaque vulnerability. However, in vivo imaging is still limited. Photoacoustic imaging may provide novel in vivo insights into these lipid-rich plaques.OBJECTIVES: This study sought to examine whether intravascular photoacoustic tomography (IVPAT) allows localization and quantification of lipid content in atherosclerotic plaques.METHODS: Rabbits fed with a high-fat/high-cholesterol diet served as the atherosclerotic model. Catheter-based IVPAT was used to evaluate pixel-based lipid relative concentration (LRC) of the vessel wall. The aorta of 4 groups of rabbits (n = 12) were examined ex vivo with IVPAT after 0, 5, 10, and 15 weeks of a high-fat diet, respectively. Six rabbits underwent 3-dimensional (3D) IVPAT after 20 weeks of the high-fat diet. Three rabbits were examined in vivo using IVPAT without interruption of blood flow. Concentration-based lipid map and quantitative index were calculated. For subsequent histologic correlation, all specimens were evaluated with Oil Red O staining.RESULTS: Cross-sectional LRC maps allowed visualization of concentration and depth information of lipid content in the atherosclerotic plaques. Lipid accumulation within plaque, assessed by the maximum LRC, mean LRC, and high lipid content area correlated to duration of a high-fat diet. Three-dimensional LRC maps enabled overall evaluation of focal plaques in an intact explanted aorta including spatial and structural features. In vivo-obtained LRC maps accurately showed the structure of lipid core with high contrast. Ex vivo and in vivo IVPAT results were highly consistent with histological results.CONCLUSIONS: In an animal model, IVPAT allowed characterization of spatial and quantitative features of lipid-rich plaques.
|
['Animals', 'Aorta, Abdominal', 'Aorta, Thoracic', 'Aortic Diseases', 'Atherosclerosis', 'Disease Models, Animal', 'Imaging, Three-Dimensional', 'Lipids', 'Magnetic Resonance Imaging', 'Male', 'Photoacoustic Techniques', 'Plaque, Atherosclerotic', 'Rabbits', 'Reproducibility of Results', 'Ultrasonography, Interventional']
| 25,060,374
|
[['B01.050'], ['A07.015.114.056.205'], ['A07.015.114.056.372'], ['C14.907.109'], ['C14.907.137.126.307'], ['C22.232', 'E05.598.500', 'E05.599.395.080'], ['E01.370.350.400', 'L01.224.308.410'], ['D10'], ['E01.370.350.825.500'], ['E01.370.565', 'E05.696'], ['C23.300.823'], ['B01.050.150.900.649.313.968.700'], ['E05.318.370.725', 'E05.337.851', 'N05.715.360.325.685', 'N06.850.520.445.725'], ['E01.370.350.850.855', 'E04.502.890']]
|
['Organisms [B]', 'Anatomy [A]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Information Science [L]', 'Chemicals and Drugs [D]', 'Health Care [N]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 1
| 0
|
Fasciolopsiasis in a five year old girl.
|
A 5 year old girl hailing from Keraniganj, presented with the complaints of fever, periumbilical pain and vomiting. In vomitus, Fasciolopsis buski worm in adult form was identified by naked eye examination. In stool, ova of Fasciolopsis buski were also observed under microscope. Clinically she was pale and had hepatomegaly. Microcytic hypochromic anaemia with normal liver function test was found on lab investigation. She was diagnosed as a case of Fasciolopsiasis and treated with Praziquantel and on follow up visit she was found to be free of symptom.
|
['Animals', 'Anthelmintics', 'Child, Preschool', 'Diagnosis, Differential', 'Female', 'Humans', 'Praziquantel', 'Trematode Infections']
| 23,715,369
|
[['B01.050'], ['D27.505.954.122.250.075'], ['M01.060.406.448'], ['E01.171'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D03.633.100.531.690'], ['C01.610.335.865']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Fine structure of psammoma bodies at the outer aspect of blood vessels in meningioma.
|
Psammoma bodies at the perivascular area in five cases of meningioma were examined with the electron microscope. In general, meningocytic cells invest the outer aspect of blood vessels, which are constituted by multilayered basal laminae, collagen fibers, microfibrils, and pericytes. Remnants of degenerated cells are observed in some areas of the perivascular space. Matrix vesicles and matrix giant bodies with or without mineralized deposits are also present in those areas. Energy dispersive, X-ray microanalysis evidenced the presence of both calcium and phosphorus (probably hydroxyapatite) mineralized precipitates. Production of psammoma bodies in the perivascular area may indicate that matrix vesicles and matrix giant bodies are derived from degenerated cells, which then sequestrate hydroxyapatite crystals to form psammoma bodies.
|
['Blood Vessels', 'Calcinosis', 'Electron Probe Microanalysis', 'Humans', 'Meningioma', 'Microscopy, Electron']
| 4,013,670
|
[['A07.015'], ['C18.452.174.130'], ['E01.370.350.515.402.250', 'E05.196.867.800.360', 'E05.595.402.250', 'E05.799.830.360'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C04.557.580.520', 'C04.557.645.520', 'C04.588.614.250.580.500', 'C10.551.240.500.500'], ['E01.370.350.515.402', 'E05.595.402']]
|
['Anatomy [A]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
RAC, a stable ribosome-associated complex in yeast formed by the DnaK-DnaJ homologs Ssz1p and zuotin.
|
The yeast cytosol contains multiple homologs of the DnaK and DnaJ chaperone family. Our current understanding of which homologs functionally interact is incomplete. Zuotin is a DnaJ homolog bound to the yeast ribosome. We have now identified the DnaK homolog Ssz1p/Pdr13p as zuotin's partner chaperone. Zuotin and Ssz1p form a ribosome-associated complex (RAC) that is bound to the ribosome via the zuotin subunit. RAC is unique among the eukaryotic DnaK-DnaJ systems, as the 1:1 complex is stable, even in the presence of ATP or ADP. In vitro, RAC stimulates the translocation of a ribosome-bound mitochondrial precursor protein into mitochondria, providing evidence for its chaperone-like effect on nascent chains. In agreement with the existence of a functional complex, deletion of each RAC subunit resulted in a similar phenotype in vivo. However, overexpression of zuotin partly rescued the growth defect of the Delta ssz1 strain, whereas overexpression of Ssz1p did not affect the Delta zuo1 strain, suggesting a pivotal function for the DnaJ homolog.
|
['Cytosol', 'DNA-Binding Proteins', 'Dimerization', 'Escherichia coli Proteins', 'Fungal Proteins', 'HSP40 Heat-Shock Proteins', 'HSP70 Heat-Shock Proteins', 'Heat-Shock Proteins', 'Mitochondria', 'Molecular Chaperones', 'Saccharomyces cerevisiae', 'Saccharomyces cerevisiae Proteins']
| 11,274,393
|
[['A11.284.430.214.200', 'A11.284.430.429.200', 'A11.284.835.450.200'], ['D12.776.260'], ['G02.206', 'G03.230'], ['D12.776.097.275'], ['D12.776.354'], ['D12.776.580.216.292'], ['D12.776.580.216.375'], ['D12.776.580.216'], ['A11.284.430.214.190.875.564', 'A11.284.835.626'], ['D12.776.580'], ['B01.300.107.795.785.800', 'B01.300.930.705.655'], ['D12.776.354.750']]
|
['Anatomy [A]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Activation of dopamine D3 receptors inhibits reward-related learning induced by cocaine.
|
Memories of learned associations between the rewarding properties of drugs and environmental cues contribute to craving and relapse in humans. The mesocorticolimbic dopamine (DA) system is involved in reward-related learning induced by drugs of abuse. DA D3 receptors are preferentially expressed in mesocorticolimbic DA projection areas. Genetic and pharmacological studies have shown that DA D3 receptors suppress locomotor-stimulant effects of cocaine and reinstatement of cocaine-seeking behaviors. Activation of the extracellular signal-regulated kinase (ERK) induced by acute cocaine administration is also inhibited by D3 receptors. How D3 receptors modulate cocaine-induced reward-related learning and associated changes in cell signaling in reward circuits in the brain, however, have not been fully investigated. In the present study, we show that D3 receptor mutant mice exhibit potentiated acquisition of conditioned place preference (CPP) at low doses of cocaine compared to wild-type mice. Activation of ERK and CaMKIIá, but not the c-Jun N-terminal kinase and p38, in the nucleus accumbens, amygdala and prefrontal cortex is also potentiated in D3 receptor mutant mice compared to that in wild-type mice following CPP expression. These results support a model in which D3 receptors modulate reward-related learning induced by low doses of cocaine by inhibiting activation of ERK and CaMKIIá in reward circuits in the brain.
|
['Animals', 'Blotting, Western', 'Brain', 'Cocaine', 'Cocaine-Related Disorders', 'Conditioning, Psychological', 'Dopamine Uptake Inhibitors', 'Enzyme Activation', 'Female', 'Learning', 'Male', 'Mice', 'Mice, Inbred C57BL', 'Mice, Mutant Strains', 'Receptors, Dopamine D3', 'Reward', 'Signal Transduction']
| 21,168,475
|
[['B01.050'], ['E05.196.401.143', 'E05.301.300.096', 'E05.478.566.320.200', 'E05.601.262', 'E05.601.470.320.200'], ['A08.186.211'], ['D02.145.074.722.388', 'D03.132.889.354', 'D03.605.084.500.722.388', 'D03.605.869.388'], ['C25.775.300', 'F03.900.300'], ['F02.463.425.179'], ['D27.505.519.562.437.220', 'D27.505.519.625.150.800', 'D27.505.519.625.600.220', 'D27.505.696.577.150.800', 'D27.505.696.577.600.220'], ['G02.111.263', 'G03.328'], ['F02.463.425', 'F02.784.629.529'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.420', 'B01.050.150.900.649.313.992.635.505.500.400.420'], ['B01.050.150.900.649.313.992.635.505.500.550'], ['D12.776.543.750.670.300.400.500.249', 'D12.776.543.750.695.150.400.500.500', 'D12.776.543.750.720.330.400.500.249'], ['F02.463.425.770.836'], ['G02.111.820', 'G04.835']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Extracellular secretion of Escherichia coli heat-stable enterotoxin I across the outer membrane.
|
Escherichia coli heat-stable enterotoxin Ip (STIp) is an extracellular toxin consisting of 18 amino acid residues that is synthesized as a precursor of pre (amino acid residues 1 to 19), pro (amino acid residues 20 to 54), and mature (amino acid residues 55 to 72) regions. The precursor synthesized in the cytoplasm is translocated across the inner membrane by the general export pathway consisting of Sec proteins. The pre region functions as a leader peptide and is cleaved during translocation. However, it remains unknown how the resulting peptide (pro-mature peptide) translocates across the outer membrane. In this study, we investigated the structure of the STIp that passes through the outer membrane to determine how it translocates through the outer membrane. The results showed that the pro region is cleaved in the periplasmic space. The generated peptide becomes the mature form of STIp, which happens to have disulfide bonds, which then passes through the outer membrane. We also showed that STIp with a carboxy-terminal peptide consisting of 3 amino acid residues passes through the outer membrane, whereas STIp with a peptide composed of 37 residues does not. Amino acid analysis of mutant STIp purified from culture supernatant revealed that the peptide composed of 37 amino acid residues was cleaved into fragments of 5 amino acid residues. In addition, analyses of STIps with a mutation at the cysteine residue and the dsbA mutant strain revealed that the formation of an intramolecular disulfide bond within STIp is not absolutely required for the mature region of STIp to pass through the outer membrane.
|
['Amino Acid Sequence', 'Bacterial Toxins', 'Base Sequence', 'Biological Transport', 'Cell Wall', 'Enterotoxins', 'Escherichia coli', 'Escherichia coli Proteins', 'Molecular Sequence Data', 'Mutation']
| 9,171,378
|
[['G02.111.570.060', 'L01.453.245.667.060'], ['D23.946.123'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['G03.143'], ['A11.284.183'], ['D23.946.330'], ['B03.440.450.425.325.300', 'B03.660.250.150.180.100'], ['D12.776.097.275'], ['L01.453.245.667'], ['G05.365.590']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Organisms [B]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Social factors affecting treatment of cervical cancer: ethical issues and policy implications.
|
Health care in the United States has become a privilege rather than a right. Patients who have the greatest need are the ones most likely to be denied this privilege. Despite recent advances in disease detection and treatment, many patients do not receive even the bare minimum of care. The high complexity of the health care system in the setting of patients with low levels of health literacy significantly affects the ability to seek and receive treatment in a timely fashion. In addition, lack of insurance, transportation, and social support further complicate access to care. To truly provide a standard of care to all patients, regardless of resources, our health care system must evolve to address the needs of the population. In this paper, we report a tragic case where social factors affected the outcome of a single mother with advanced cervical cancer.
|
['Adult', 'African Americans', 'Diagnostic Errors', 'Fatal Outcome', 'Female', 'Health Services Accessibility', 'Healthcare Disparities', 'Humans', 'Medicaid', 'Neoplasms, Squamous Cell', 'Social Support', 'State Health Plans', 'United States', 'Uterine Cervical Neoplasms']
| 18,310,380
|
[['M01.060.116'], ['M01.686.508.100.100', 'M01.686.754.100'], ['E01.354', 'N02.421.450.280'], ['E05.318.308.985.550.325', 'N01.224.935.698.201', 'N06.850.505.400.975.550.325', 'N06.850.520.308.985.550.325'], ['N04.590.374.350', 'N05.300.430'], ['N04.590.374.380', 'N05.300.493'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['N03.219.521.346.506.564.655', 'N03.706.615.693'], ['C04.557.470.700'], ['I01.880.853.500.600'], ['N03.349.650.480'], ['Z01.107.567.875'], ['C04.588.945.418.948.850', 'C13.351.500.852.593.131', 'C13.351.500.852.762.850', 'C13.351.937.418.875.850']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Diseases [C]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Geographicals [Z]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 1
|
Direct asymmetric aldol reactions of acetone using bimetallic zinc catalysts.
|
[reaction: see text] The enantioselective aldol reaction using a novel binuclear zinc catalyst of acetone with several aldehydes gave products in good yields (62-89%) with a high level of enantioselectivity (ee = 76-92%).
|
['Acetone', 'Catalysis', 'Stereoisomerism', 'Zinc']
| 11,483,044
|
[['D02.522.064'], ['G02.130'], ['G02.607.445.682'], ['D01.268.556.940', 'D01.268.956.906', 'D01.552.544.940']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]']
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Ambient temperature detection of PCR amplicons with a novel sequence-specific nucleic acid lateral flow biosensor.
|
In the field of diagnostics, molecular amplification targeting unique genetic signature sequences has been widely used for rapid identification of infectious agents, which significantly aids physicians in determining the choice of treatment as well as providing important epidemiological data for surveillance and disease control assessment. We report the development of a rapid nucleic acid lateral flow biosensor (NALFB) in a dry-reagent strip format for the sequence-specific detection of single-stranded polymerase chain reaction (PCR) amplicons at ambient temperature (22-25°C). The NALFB was developed in combination with a linear-after-the-exponential PCR assay and the applicability of this biosensor was demonstrated through detection of the cholera toxin gene from diarrheal-causing toxigenic Vibrio cholerae. Amplification using the advanced asymmetric PCR boosts the production of fluorescein-labeled single-stranded amplicons, allowing capture probes immobilized on the NALFB to hybridize specifically with complementary targets in situ on the strip. Subsequent visual formation of red lines is achieved through the binding of conjugated gold nanoparticles to the fluorescein label of the captured amplicons. The visual detection limit observed with synthetic target DNA was 0.3 ng and 1 pg with pure genomic DNA. Evaluation of the NALFB with 164 strains of V. cholerae and non-V. cholerae bacteria recorded 100% for both sensitivity and specificity. The whole procedure of the low-cost NALFB, which is performed at ambient temperature, eliminates the need for preheated buffers or additional equipment, greatly simplifying the protocol for sequence-specific PCR amplicon analysis.
|
['Base Sequence', 'Biosensing Techniques', 'Cholera', 'Cholera Toxin', 'DNA, Bacterial', 'Gold', 'Humans', 'Limit of Detection', 'Molecular Sequence Data', 'Nanoparticles', 'Polymerase Chain Reaction', 'Reagent Strips', 'Sensitivity and Specificity', 'Temperature', 'Vibrio cholerae']
| 22,705,404
|
[['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['E05.601.043'], ['C01.150.252.400.959.347'], ['D08.811.913.400.725.115.180', 'D23.946.123.194', 'D23.946.330.150'], ['D13.444.308.212'], ['D01.268.556.322', 'D01.268.956.186', 'D01.552.544.322'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.740.872.374', 'N05.715.360.750.725.500', 'N06.850.520.830.872.500'], ['L01.453.245.667'], ['J01.637.512.600'], ['E05.393.620.500'], ['D27.505.259.875.680', 'D27.720.470.410.680.680', 'E07.720.720'], ['E05.318.370.800', 'E05.318.740.872', 'G17.800', 'N05.715.360.325.700', 'N05.715.360.750.725', 'N06.850.520.445.800', 'N06.850.520.830.872'], ['G01.906.595', 'G16.500.275.063.725.710', 'G16.500.750.775.710', 'N06.230.150.450', 'N06.230.300.100.725.710'], ['B03.440.450.900.859.225', 'B03.660.250.830.830.100']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Health Care [N]', 'Technology, Industry, and Agriculture [J]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 1
| 0
|
[A population survey on bone mineral density in a fishing village in Wakayama Prefecture (Part 2); The analysis of the risk factors affecting the bone mineral density].
|
The purpose of this study was to detect factors affecting lumbar bone mineral density (BMD) of general inhabitants in a rural community. A cohort consisting of 2261 inhabitants aged 40-79 years was set up based on the resident registration in Taiji town, Wakayama Prefecture in 1992. Fifty men and 50 women in each of four age strata (40-49, 50-59, 60-69, 70-79), totaling 400 inhabitants, were selected randomly. After completion of a questionnaire by an interviewer, items about physical characteristics such as height, body weight, wrist length and grip power were measured. Examination of BMD of the lumbar spine and proximal femur by dual energy X-ray absorptiometry was performed. Correlation coefficients between BMDs and values of physical characteristics were determined. Lumbar BMD was examined to determine whether the following factors were positive or negative: past history, alcohol consumption, smoking habit, dietary habit, exercise, and in addition, pregnancy times and menstrual status for women. Among the values of physical characteristics, body weight was the most closely correlated with lumbar BMD. The items that showed significantly high values as factors affecting lumbar BMD were a past history of diabetes mellitus (men and women in their 70s), and exercise (men in their 50s and women in their 40s). Regarding pregnancy times and menstruation, BMD in women with a history of childbirth was significantly higher than that in those without the history among the women in their 70s. Although the BMD in the women with lactation was significantly lower than that in those without it among the women in their 40s, there was no difference in BMD between the women with and without the history who were 50 years old or over. The effect of menstrual status was investigated according to years after menopause in the women in their 50s. BMD was significantly lower in the women with ar least six years after menopause than in those within five years.
|
['Adolescent', 'Adult', 'Aged', 'Body Composition', 'Bone Density', 'Cohort Studies', 'Diabetes Mellitus', 'Exercise', 'Female', 'Fisheries', 'Humans', 'Japan', 'Male', 'Middle Aged', 'Population Surveillance', 'Pregnancy', 'Risk Factors', 'Rural Population']
| 8,952,327
|
[['M01.060.057'], ['M01.060.116'], ['M01.060.116.100'], ['G02.111.130', 'G03.180', 'G07.100.049'], ['G11.427.100'], ['E05.318.372.500.750', 'N05.715.360.330.500.750', 'N06.850.520.450.500.750'], ['C18.452.394.750', 'C19.246'], ['G11.427.410.698.277', 'I03.350'], ['J01.040.168.300', 'J03.540.280'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['Z01.252.474.463', 'Z01.639.595'], ['M01.060.116.630'], ['E05.318.308.980.438.700', 'N05.715.360.300.800.438.625', 'N06.850.520.308.980.438.700', 'N06.850.780.675'], ['G08.686.784.769'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['N01.600.725']]
|
['Named Groups [M]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Diseases [C]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Technology, Industry, and Agriculture [J]', 'Organisms [B]', 'Geographicals [Z]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 1
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Efficacy of ivermectin against cutaneous Draschia and Habronema infection (summer sores) in horses.
|
Thirty-one horses with naturally occurring summer sores were given a single IM injection of 0.2 mg of ivermectin (22,23-dihydroavermectin B1)/kg during the summer of 1980. Larvae of Draschia and/or Habronema spp were recovered from biopsy samples taken from 21 of 25 horses (84%) on the day of anthelmintic treatment. There was a marked clinical improvement in 26 horses (84%) 7 days after ivermectin was given. The typical summer sore was replaced by healthy pink granulation tissue at 7 days and this healed after 1 to 5 weeks. Biopsy samples were taken from 21 of these horses 1 to 6 weeks after treatment, and the samples in 18 horses (86%) were negative for larvae. Five of the 31 infected horses did not respond to a single dose and appeared to have become reinfected. Biopsy samples taken from 3 of these horses were positive for larvae 2 to 4 weeks after the 1st dose, but became negative and healed 1 to 2 weeks after a 2nd dose with ivermectin. Histopathologic examination of sections from lesions before anthelmintic treatment showed granulation tissue with marked eosinophilic infiltration, multiple eosinophilic abscesses, and transverse sections of nematodes. In sections examined after anthelmintic treatment, there were marked reduction in the number of eosinophils and absence of eosinophilic abscesses or nematodes, and the granulation tissue was more mature, as indicated by the appearance of dense fibrous connective tissue.
|
['Animals', 'Antiparasitic Agents', 'Horses', 'Ivermectin', 'Lactones', 'Larva', 'Nematode Infections', 'Seasons', 'Skin Diseases, Parasitic', 'Spirurida Infections', 'Spiruroidea']
| 6,461,283
|
[['B01.050'], ['D27.505.954.122.250'], ['B01.050.150.900.649.313.984.235.472'], ['D02.540.576.500.997'], ['D02.540'], ['B05.500.500', 'G07.345.500.550.500.500'], ['C01.610.335.508'], ['G01.910.645.661', 'G16.500.275.071.590', 'N06.230.300.100.250.525'], ['C01.610.858', 'C17.800.838.775'], ['C01.610.335.508.700.750'], ['B01.050.500.500.294.400.937.700.680']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Health Care [N]']
| 0
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
[Can we improve the general and nutritional management of elderly individuals living at a medical-social facility? A team work].
|
Can we improve comprehensive care of old people's home residents? Working in an interdisciplinary health network, health professionals tried to better answer the real needs of residents. Malnutrition is is highly prevalent in this population. We showed that it is possible to improve health care of these patients by better organizing meals with a more enriched protein content provided a better involvement in the process of every caring team member. Most of the new residents improved their nutritional state already after the first months in institution. However we still have to develop managerial tools to avoid routine, evaluate the results on the long term and stimulate caregivers as there is a clear relationship between health quality, nutritional state and survival in these old people. On the other hand, a better medical follow up of antibiotherapy allowed a 30% decrease in antibiotics consumption. We also showed a better acceptance of influenza vaccination with a vaccination prevalence going from 80% to 99% in residents and from 80% to 100% in caregivers. Such an approach shows that health care can be improved in old people's homes provided interdisciplinary coordinated work between physicians, nurses and other professionals.
|
['Aged', 'Anti-Bacterial Agents', 'Diet', 'Geriatrics', 'Homes for the Aged', 'Humans', 'Nutritional Status', 'Switzerland']
| 15,095,708
|
[['M01.060.116.100'], ['D27.505.954.122.085'], ['G07.203.650.240'], ['H02.403.355'], ['J03.775.462', 'N02.278.825.462'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G07.203.650.650', 'N01.224.425.525'], ['Z01.542.883']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Disciplines and Occupations [H]', 'Technology, Industry, and Agriculture [J]', 'Health Care [N]', 'Organisms [B]', 'Geographicals [Z]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 1
| 1
|
Lin28b reprograms adult bone marrow hematopoietic progenitors to mediate fetal-like lymphopoiesis.
|
The immune system develops in waves during ontogeny; it is initially populated by cells generated from fetal hematopoietic stem cells (HSCs) and later by cells derived from adult HSCs. Remarkably, the genetic programs that control these two distinct stem cell fates remain poorly understood. We report that Lin28b is specifically expressed in mouse and human fetal liver and thymus, but not in adult bone marrow or thymus. We demonstrate that ectopic expression of Lin28 reprograms hematopoietic stem/progenitor cells (HSPCs) from adult bone marrow, which endows them with the ability to mediate multilineage reconstitution that resembles fetal lymphopoiesis, including increased development of B-1a, marginal zone B, gamma/delta (ãä) T cells, and natural killer T (NKT) cells.
|
['Adult Stem Cells', 'Animals', 'B-Lymphocyte Subsets', 'B-Lymphocytes', 'Bone Marrow Cells', 'Cell Lineage', 'DNA-Binding Proteins', 'Fetal Blood', 'Fetus', 'Flow Cytometry', 'Hematopoietic Stem Cells', 'Humans', 'Liver', 'Lymphopoiesis', 'Mice', 'Mice, Inbred C57BL', 'MicroRNAs', 'Natural Killer T-Cells', 'RNA-Binding Proteins', 'Reverse Transcriptase Polymerase Chain Reaction', 'T-Lymphocyte Subsets', 'T-Lymphocytes', 'Thymus Gland', 'Transduction, Genetic']
| 22,345,399
|
[['A11.872.040'], ['B01.050'], ['A11.063.438.450', 'A11.118.637.555.567.550.450', 'A11.118.637.555.567.562.200', 'A15.145.229.637.555.567.550.450', 'A15.145.229.637.555.567.562.200', 'A15.382.032.438.450', 'A15.382.490.555.567.550.300', 'A15.382.490.555.567.562.450'], ['A11.063.438', 'A11.118.637.555.567.562', 'A15.145.229.637.555.567.562', 'A15.382.032.438', 'A15.382.490.555.567.562'], ['A11.148', 'A15.378.316'], ['G04.172', 'G07.345.500.325.180.500', 'G08.686.155', 'G08.686.784.170.104.249'], ['D12.776.260'], ['A12.207.152.200', 'A15.145.300', 'A16.378.200'], ['A16.378'], ['E01.370.225.500.363.342', 'E01.370.225.500.386.350', 'E05.196.712.516.600.240.350', 'E05.200.500.363.342', 'E05.200.500.386.350', 'E05.242.363.342', 'E05.242.386.350'], ['A11.148.378', 'A11.872.378', 'A15.378.316.378'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A03.620'], ['G04.152.825.597.500', 'G09.188.343.597.500'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.420', 'B01.050.150.900.649.313.992.635.505.500.400.420'], ['D13.150.650.319', 'D13.444.735.150.319', 'D13.444.735.790.552.500'], ['A11.118.637.555.567.569.290', 'A15.145.229.637.555.567.569.360', 'A15.382.490.555.567.569.470'], ['D12.776.157.725', 'D12.776.664.962'], ['E05.393.620.500.725'], ['A11.118.637.555.567.550.500', 'A11.118.637.555.567.569.500', 'A15.145.229.637.555.567.550.500', 'A15.145.229.637.555.567.569.500', 'A15.382.490.555.567.550.500', 'A15.382.490.555.567.569.500'], ['A11.118.637.555.567.569', 'A15.145.229.637.555.567.569', 'A15.382.490.555.567.569'], ['A10.549.750', 'A15.382.520.604.750'], ['E05.393.350.800', 'G05.728.850']]
|
['Anatomy [A]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[Transient hyperglycemia following intra-peritoneal irrigation with 5% glucose in a patient with pseudomyxoma peritonei].
|
Pseudomyxoma peritonei is a condition characterized by the production of a large amount of mucopolysaccharide by a neoplastic epithelium. Although surgical removal of the mucinous ascites may be attempted, complete removal of the material is difficult. Thus, intra-peritoneal lavage with the liquid containing glucose or dextrose has been advocated to prevent reaccumulation of the mucus and complications such as bowel obstruction requiring repeated surgery. We report a case showing transient hyperglycemia following intra-peritoneal irrigation with 5% glucose in a patient with psudomyxoma peritonei. The patient was a 72-year-old woman. Preoperatively, she had hypertension and angina pectoris; but no history of glucose intolerance. Serum glucose was 92 mg x dl(-1). General anesthesia was induced with propofol (100 mg), vecuronium (6 mg), and fentanyl, and maintained with oxygen (33%), nitrous oxide and sevoflurane (1-2%). A mucinous tumor was found with a great deal of mucinous ascites. To remove the mucus and prevent subsequent re-accumulation, intra-peritoneal irrigation with 5% glucose in water was performed. Shortly after this procedure, the patient was found to be hyperglycemic (serum glucose 266 mg x dl(-1)) with normal oxygenation and hemodynamic data. The patient recovered uneventfully and could be extubated soon after surgery. Serum glucose level returned to 154 mg x dl(-1) one hour after surgery. Therefore, we think that this acute hyperglycemic condition, presumable due to intra-peritoneal irrigation, was transient. It is important to be aware of this dangerous complication associated with intra-peritoneal glucose instillation. Glucose monitoring during and after irrigation with glucose or dextrose is recommended.
|
['Aged', 'Anesthesia, General', 'Blood Glucose', 'Female', 'Glucose', 'Humans', 'Hyperglycemia', 'Monitoring, Physiologic', 'Peritoneal Lavage', 'Postoperative Complications', 'Pseudomyxoma Peritonei']
| 17,715,692
|
[['M01.060.116.100'], ['E03.155.197'], ['D09.947.875.359.448.500'], ['D09.947.875.359.448'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C18.452.394.952'], ['E01.370.520'], ['E05.927.705'], ['C23.550.767'], ['C04.557.470.200.025.075.500', 'C04.557.470.590.075.500']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Nifedipine aerosol attenuates airway constriction in dogs with hyperreactive airways.
|
We investigated the ability of a calcium channel blocker nifedipine, given as an aerosol, to attenuate bronchoconstriction induced by citric acid, Ascaris antigen, and methacholine in Basenji-Greyhound dogs. citric acid 10% increased pulmonary resistance (RL) 4.2-fold +/- 1.0 (mean +/- SEM) and 1.7-fold +/- 0.7 in untreated and nifedipine-pretreated dogs (P less than 0.05). Dynamic compliance (Cdyn) fell to 0.58 +/- 0.07 and 0.66 +/- 0.07 of control values in untreated and nifedipine-treated dogs, respectively (p greater than 0.05). Ascaris antigen increased RL 5.1-fold +/- 0.83 and 3-fold +/- 0.48 in untreated and nifedipine-treated dogs, respectively (p less than 0.05); Cdyn decreased to 0.33 +/- 0.04 and 0.48 +/- 0.03 in untreated and nifedipine-treated dogs, respectively (p less than 0.05). In 5 dogs challenged with 0.3 mg/ml methacholine, RL increased 6.1-fold +/- 0.7 and 4.4-fold +/- 1.0 in untreated and nifedipine-treated dogs, respectively (p less than 0.05); Cdyn fell to 0.41 +/- 0.-3 and 0.46 +/- 0.07 of control values in untreated and nifedipine-treated dogs (P greater than 0.05). Neither 40% ethanol nor nifedipine-ethanol altered resting RL and Cdyn. We conclude that nifedipine effectively attenuates bronchoconstriction induced by citric acid, Ascaris antigen, and methacholine in dogs with hyperreactive airways.
|
['Aerosols', 'Airway Resistance', 'Animals', 'Asthma', 'Bronchi', 'Bronchial Provocation Tests', 'Constriction, Pathologic', 'Dogs', 'Lung Compliance', 'Nifedipine', 'Pyridines']
| 6,849,541
|
[['D20.280.055', 'D26.255.165.055'], ['E01.370.386.700.050', 'G09.772.060'], ['B01.050'], ['C08.127.108', 'C08.381.495.108', 'C08.674.095', 'C20.543.480.680.095'], ['A04.411.125'], ['E01.370.386.700.125'], ['C23.300.287'], ['B01.050.150.900.649.313.750.250.216.200'], ['E01.370.386.700.475', 'G09.772.540'], ['D03.383.725.203.540'], ['D03.383.725']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Diseases [C]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Heat shock induces the synthesis of the inflammatory mediator leukotriene B4 in human pulp cells.
|
AIM: To measure the synthesis of leukotriene B4 (LTB4) in cultures of human dental pulp cells induced by heat shock.METHODOLOGY: Primary pulp cells (PC) and dental pulp stem cells (DPSC) were cultivated under appropriate conditions. For the characterization of PC the expression of dentine sialophosphoprotein (DSPP) was evaluated by reverse transcription-polymerase chain reaction. Thermal stimulation of cell cultures was performed at temperatures of 37, 38, 39, 40, 42 and 45 degrees C for stimulation times of 5 and 30 s. LTB4 was quantified by reversed-phase high-performance chromatography and differences between the LTB4 concentrations of controls and heat stimulated cells were analysed with Friedman analysis of variances by ranks and multiple comparisons (P < 0.05).RESULTS: Both cell cultures expressed DSPP under the conditions of the present experiment. The analysis revealed significantly enhanced LTB4 synthesis following thermal stimulations at 38, 39, 40, 42 and 45 degrees C compared with unstimulated controls for both PC and DPSC.CONCLUSION: The present study demonstrated the capability of pulp cells to synthesize the arachidonic acid mediator LTB4 in response to heat shock. LTB4 has the capacity to induce inflammatory reactions and to sensitise afferent nociceptive nerve endings. LTB4 synthesis is induced by minor temperature changes, which are relevant for various clinical situations.
|
['Cells, Cultured', 'Dental Pulp', 'Extracellular Matrix Proteins', 'Hot Temperature', 'Humans', 'Inflammation Mediators', 'Leukotriene B4', 'Phosphoproteins', 'Protein Precursors', 'Sialoglycoproteins', 'Stem Cells', 'Time Factors']
| 16,343,115
|
[['A11.251'], ['A14.549.167.900.260'], ['D12.776.860.300'], ['G01.906.595.543', 'G16.500.275.063.725.710.380', 'G16.500.750.775.710.380', 'N06.230.300.100.725.232', 'N06.230.300.100.725.710.380'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D23.469'], ['D10.251.355.255.100.450.411', 'D10.251.355.310.166.887.411', 'D23.469.050.175.450.415'], ['D12.776.744'], ['D12.776.811'], ['D12.644.233.800', 'D12.776.395.700'], ['A11.872'], ['G01.910.857']]
|
['Anatomy [A]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Organisms [B]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
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