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Microsporidial keratoconjunctivitis after rugby tournament, Singapore.
|
We investigated an outbreak of 47 probable and 6 confirmed cases of microsporidial keratoconjunctivitis involving participants of an international rugby tournament in Singapore in April 2012.The mode of transmission was eye contact with soil. Vittaforma corneae was identified in 4 of 6 corneal scrapings and in 1 of 12 soil water samples.
|
['Adolescent', 'Child', 'Disease Outbreaks', 'Female', 'Football', 'Humans', 'Keratoconjunctivitis', 'Male', 'Microsporidia', 'Microsporidiosis', 'Polymerase Chain Reaction', 'Singapore']
| 23,965,938
|
[['M01.060.057'], ['M01.060.406'], ['N06.850.290'], ['I03.450.642.845.300'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C11.187.183.394', 'C11.204.564.585'], ['B01.300.360'], ['C01.150.703.617'], ['E05.393.620.500'], ['Z01.252.145.774']]
|
['Named Groups [M]', 'Health Care [N]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Organisms [B]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Geographicals [Z]']
| 0
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| 1
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| 1
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Severe Burn-Induced Inflammation and Remodeling of Achilles Tendon in a Rat Model.
|
Severe burn causes systemic inflammation and hypercatabolism, resulting in damage to multiple organs distant to the burn site, including the musculoskeletal system. Bone mass and muscle loss have been reported. However, tendon that connects bone and muscle has not been studied in comparable detail. Here we aimed to characterize the molecular and functional changes in Achilles tendon triggered by severe burn. Forty male Sprague-Dawley rats received 40% total body surface area scald burn. Achilles tendons were collected up to 14 days postburn. Sham-treated animals served as a control group. We analyzed tendons for changes in expression of IL-6, IL-1â, TNF, MMP9, MMP13, TGFâ1, Collagens I and III, and for morphological and biomechanical changes. Gene expression of IL-6 and IL-1â as well as MMP9 and MMP13 increased in rat tendon 3 days after burn. Col3a1 increased at day 3 and col1a1 at day 7. At day 14, TGFâ1 increased, whereas the protein ratio for collagens I/III decreased, indicating tendon remodeling. Histological analysis with H&E and Picrosirius red staining further revealed a decrease in organized collagen fibers 14 days after burn. Biomechanical analysis showed a decrease in stiffness and ultimate force of tendons in burn rats.We conclude that tendinopathy was observed in Achilles tendon 14 days after severe burn, via the induction of inflammation and remodeling. The present study provides a model of tendinopathy that may be used for the development of therapeutic approaches after burn.
|
['Achilles Tendon', 'Animals', 'Burns', 'Collagen Type I', 'Collagen Type III', 'Cytokines', 'Disease Models, Animal', 'Inflammation', 'Male', 'Matrix Metalloproteinase 13', 'Matrix Metalloproteinase 9', 'Rats', 'Rats, Sprague-Dawley', 'Trauma Severity Indices']
| 29,065,066
|
[['A02.880.176'], ['B01.050'], ['C26.200'], ['D05.750.078.280.300.100', 'D12.776.860.300.250.300.100'], ['D05.750.078.280.300.300', 'D12.776.860.300.250.300.300'], ['D12.644.276.374', 'D12.776.467.374', 'D23.529.374'], ['C22.232', 'E05.598.500', 'E05.599.395.080'], ['C23.550.470'], ['D08.811.277.656.300.480.205.363', 'D08.811.277.656.300.480.525.700.550', 'D08.811.277.656.675.374.205.363', 'D08.811.277.656.675.374.525.700.550', 'D12.644.276.848.550', 'D12.776.467.836.550'], ['D08.811.277.656.300.480.205.360', 'D08.811.277.656.300.480.252.445', 'D08.811.277.656.300.480.525.700.350', 'D08.811.277.656.675.374.205.360', 'D08.811.277.656.675.374.252.445', 'D08.811.277.656.675.374.525.700.350', 'D12.644.276.848.350', 'D12.776.467.836.350'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.750'], ['E05.318.308.940.968.875', 'E05.944', 'N04.452.859.564.800', 'N05.715.360.300.715.500.800', 'N06.850.520.308.940.968.875']]
|
['Anatomy [A]', 'Organisms [B]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
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| 1
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|
Measurement of PIP3 levels reveals an unexpected role for p110â in early adaptive responses to p110á-specific inhibitors in luminal breast cancer.
|
BYL719, which selectively inhibits the alpha isoform of the phosphatidylinositol 3-kinase (PI3K) catalytic subunit (p110a), is currently in clinical trials for the treatment of solid tumors, especially luminal breast cancers with PIK3CA mutations and/or HER2 amplification. This study reveals that, even among these sensitive cancers, the initial efficacy of p110á inhibition is mitigated by rapid re-accumulation of the PI3K product PIP3 produced by the p110â isoform. Importantly, the reactivation of PI3K mediated by p110â does not invariably restore AKT phosphorylation, demonstrating the limitations of using phospho-AKT as a surrogate to measure PI3K activation. Consistently, we show that the addition of the p110â inhibitor to BYL719 prevents the PIP3 rebound and induces greater antitumor efficacy in HER2-amplified and PIK3CA mutant cancers.
|
['Animals', 'Antineoplastic Combined Chemotherapy Protocols', 'Breast Neoplasms', 'Cell Line, Tumor', 'Class I Phosphatidylinositol 3-Kinases', 'Female', 'Gene Expression Regulation, Neoplastic', 'Humans', 'MCF-7 Cells', 'Mice', 'Mice, Nude', 'Neoplasm Transplantation', 'Pyrimidinones', 'Receptor, ErbB-2', 'Signal Transduction', 'Thiazoles', 'ortho-Aminobenzoates']
| 25,544,637
|
[['B01.050'], ['E02.183.750.500', 'E02.319.077.500', 'E02.319.310.037'], ['C04.588.180', 'C17.800.090.500'], ['A11.251.210.190', 'A11.251.860.180'], ['D08.811.913.696.620.500.100.100', 'D08.811.913.696.620.500.200.100', 'D12.776.476.162'], ['G05.308.370'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A11.251.210.190.630'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.150.900.649.313.992.635.505.500.550.500'], ['E05.624'], ['D03.383.742.698'], ['D08.811.913.696.620.682.725.400.009.400', 'D12.776.543.750.630.009.400', 'D12.776.543.750.750.400.074.400', 'D12.776.624.664.700.642', 'D23.050.301.500.600.700', 'D23.050.705.552.600.550', 'D23.101.140.642'], ['G02.111.820', 'G04.835'], ['D02.886.675', 'D03.383.129.708'], ['D02.241.223.100.050.400', 'D02.455.426.559.389.127.020.906']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
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| 0
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| 0
|
Wnt9a Promotes Renal Fibrosis by Accelerating Cellular Senescence in Tubular Epithelial Cells.
|
Cellular senescence is associated with renal disease progression, and accelerated tubular cell senescence promotes the pathogenesis of renal fibrosis. However, the underlying mechanism is unknown. We assessed the potential role of Wnt9a in tubular cell senescence and renal fibrosis. Compared with tubular cells of normal subjects, tubular cells of humans with a variety of nephropathies and those of several mouse models of CKD expressed high levels of Wnt9a that colocalized with the senescence-related protein p16INK4A Wnt9a expression level correlated with the extent of renal fibrosis, decline of eGFR, and expression of p16INK4A Furthermore, ectopic expression of Wnt9a after ischemia-reperfusion injury (IRI) induced activation of â-catenin and exacerbated renal fibrosis. Overexpression of Wnt9a exacerbated tubular senescence, evidenced by increased detection of p16INK4A expression and senescence-associated â-galactosidase activity. Conversely, shRNA-mediated knockdown of Wnt9a repressed IRI-induced renal fibrosis in vivo and impeded the growth of senescent tubular epithelial cells in culture. Notably, Wnt9a-induced renal fibrosis was inhibited by shRNA-mediated silencing of p16INK4A in the IRI mouse model. In a human proximal tubular epithelial cell line and primary renal tubular cells, Wnt9a remarkably upregulated levels of senescence-related p16INK4A, p19ARF, p53, and p21 and decreased the phosphorylation of retinoblastoma protein. Wnt9a also induced senescent tubular cells to produce TGF-â1, which promoted proliferation and activation in normal rat kidney fibroblasts. Thus, Wnt9a drives tubular senescence and fibroblast activation. Furthermore, the Wnt9a-TGF-â pathway appears to create a reciprocal activation loop between senescent tubular cells and activated fibroblasts that promotes and accelerates the pathogenesis of renal fibrosis.
|
['Animals', 'Cell Line', 'Cellular Senescence', 'Cyclin-Dependent Kinase Inhibitor p16', 'Disease Models, Animal', 'Epithelial Cells', 'Fibroblasts', 'Fibrosis', 'Gene Expression Regulation', 'Genes, p16', 'Humans', 'Kidney', 'Kidney Tubules', 'Male', 'Mice', 'Mice, Inbred C57BL', 'RNA Interference', 'Rats', 'Recombinant Proteins', 'Renal Insufficiency, Chronic', 'Reperfusion Injury', 'Transforming Growth Factor beta', 'Tumor Suppressor Proteins', 'Wnt Proteins', 'Wnt Signaling Pathway']
| 29,440,280
|
[['B01.050'], ['A11.251.210'], ['G04.043'], ['D12.644.360.225.200', 'D12.776.167.187.200', 'D12.776.476.225.200', 'D12.776.624.776.355.200'], ['C22.232', 'E05.598.500', 'E05.599.395.080'], ['A11.436'], ['A11.329.228'], ['C23.550.355'], ['G05.308'], ['G05.360.340.024.340.375.249.375', 'G05.360.340.024.340.415.400.375'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A05.810.453'], ['A05.810.453.736.560'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.420', 'B01.050.150.900.649.313.992.635.505.500.400.420'], ['G05.308.203.374.790'], ['B01.050.150.900.649.313.992.635.505.700'], ['D12.776.828'], ['C12.777.419.780.750', 'C13.351.968.419.780.750'], ['C14.907.725', 'C23.550.767.877'], ['D12.644.276.374.687', 'D12.644.276.954.775', 'D12.776.467.374.687', 'D12.776.467.942.775', 'D23.529.374.687', 'D23.529.942.775'], ['D12.776.624.776'], ['D12.776.467.984', 'D23.529.984'], ['G02.111.820.925', 'G04.835.925']]
|
['Organisms [B]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
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|
The two dimensions of the body representation in women suffering from Anorexia Nervosa.
|
A core symptom of Anorexia Nervosa (AN) is a severe alteration of body representations. Evidence from somatoperception studies point to a generic disturbances of somatosensory components of body representations. Here we have investigated whether AN patients (N=18) and controls differed in the perception of tactile stimuli differently oriented along the body axes. We tested the hypothesis that patients perceive and represent their body selectively larger in only one dimension. To this aim we used elementary tactile measures for tactile acuity (Von Frey's test and two-point discrimination thresholds - 2PD) and tactile discrimination measures. The rationale is based on the assumption that AN patients have a wider body representation, and that tactile body representation tasks (Tactile Distance task) oriented across the bodies (horizontally) are influenced by distorted body representations compared with tactile stimuli oriented along the bodies (vertically) which should not be influenced by body representations. Results showed that patients judged horizontal tactile stimuli significantly wider than the same stimuli oriented vertically.These results suggest that human brain perceives things differently based on body representations and that the beliefs concerning body size influence the specific somatosensory process of tactile experience.
|
['Adult', 'Anorexia Nervosa', 'Body Image', 'Body Size', 'Female', 'Humans', 'Physical Stimulation', 'Touch', 'Touch Perception', 'Young Adult']
| 26,360,978
|
[['M01.060.116'], ['F03.400.125'], ['F01.752.747.792.110', 'F02.463.593.112'], ['E01.370.600.115.100.160', 'E05.041.124.160', 'G07.100.100.160', 'G07.345.249.314'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.723'], ['F02.830.816.850', 'G11.561.790.850'], ['F02.463.593.894'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Psychiatry and Psychology [F]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 0
| 1
| 0
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Photoresponsive surface molecularly imprinted polymer on ZnO nanorods for uric acid detection in physiological fluids.
|
A photoresponsive surface molecularly imprinted polymer for uric acid in physiological fluids was fabricated through a facile and effective method using bio-safe and biocompatible ZnO nanorods as a support. The strategy was carried out by introducing double bonds on the surface of the ZnO nanorods with 3-methacryloxypropyltrimethoxysilane. The surface molecularly imprinted polymer on ZnO nanorods was then prepared by surface polymerization using uric acid as template, water-soluble 5-[(4-(methacryloyloxy)phenyl)diazenyl]isophthalic acid as functional monomer, and triethanolamine trimethacryl ester as cross-linker. The surface molecularly imprinted polymer on ZnO nanorods showed good photoresponsive properties, high recognition ability, and fast binding kinetics toward uric acid, with a dissociation constant of 3.22?10(-5)M in aqueous NaH2PO4 buffer at pH=7.0 and a maximal adsorption capacity of 1.45ìmolg(-1). Upon alternate irradiation at 365 and 440nm, the surface molecularly imprinted polymer on ZnO nanorods can quantitatively uptake and release uric acid.
|
['Adsorption', 'Kinetics', 'Methacrylates', 'Molecular Imprinting', 'Nanotubes', 'Polymers', 'Silanes', 'Spectroscopy, Fourier Transform Infrared', 'Uric Acid', 'Water', 'Zinc Oxide']
| 27,207,036
|
[['G01.030', 'G02.020'], ['G01.374.661', 'G02.111.490'], ['D02.241.081.069.600'], ['E05.196.655', 'E05.197.453', 'J01.897.836.249.437'], ['J01.637.512.850'], ['D05.750', 'D25.720', 'J01.637.051.720'], ['D01.837.700'], ['E05.196.712.726.676.700', 'E05.196.867.826.676.700'], ['D03.132.960.877', 'D03.633.100.759.758.824.877'], ['D01.045.250.875', 'D01.248.497.158.459.650', 'D01.650.550.925'], ['D01.650.550.975', 'D01.975.975']]
|
['Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Technology, Industry, and Agriculture [J]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Experimental infection of non-pregnant and pregnant sheep with Neospora caninum.
|
In an initial experiment, 21 sheep in groups of five or six were inoculated subcutaneously (sc) with 10(8), 10(6) or 10(4) Neospora caninum tachyzoites (Liverpool isolate), or with control inoculum, and monitored for clinical signs and for "seroconversion". Animals given the two higher doses showed febrile responses and all three groups inoculated with the parasite showed seroconversion. In a second experiment, 12 pregnant sheep were each inoculated sc at 90 days' gestation with 10(6) tachyzoites, and at 25, 40 and 53 days post-inoculation (dpi) groups of four were killed for examination of the fetuses and placentas. Appropriate control ewes were included in the study. All fetuses were alive immediately before their dams were killed, except for one, which was found to be mummified at 40 dpi. Histopathological lesions were found consistently in both fetal central nervous system (CNS) and placental tissues. In the latter, focal necrosis, which was mild at 25 dpi, was much more severe at 40 dpi and much less severe at 53 dpi. Lesions in the fetal CNS consisted of focal microgliosis (with or without central necrosis), lymphoid cuffing and non-suppurative meningitis. Lesions were also found in fetal liver, heart and lung. Neospora antigen was demonstrated in fetal brain and placental tissues and, at 25 dpi, in single samples of fetal liver and heart. The prescapular lymph nodes did not differ in size from those of control fetuses but were more mature in that they contained a significantly greater number of secondary follicles. Both IgM and IgG antibodies to N. caninum were detected in the serum of fetuses from infected ewes. Thus, N. caninum readily infected pregnant ewes and caused lesions in fetal tissues and placentas which resembled those of ovine toxoplasmosis. In addition, the changes were similar to those of bovine neosporosis; the infected pregnant ewe therefore offers a good model for the bovine disease.
|
['Animals', 'Antibodies, Protozoan', 'Antigens, Protozoan', 'Body Temperature', 'Brain', 'Female', 'Male', 'Neospora', 'Placenta', 'Pregnancy', 'Protozoan Infections', 'Protozoan Infections, Animal', 'Sheep', 'Time Factors']
| 9,263,840
|
[['B01.050'], ['D12.776.124.486.485.114.252', 'D12.776.124.790.651.114.252', 'D12.776.377.715.548.114.252'], ['D23.050.293'], ['E01.370.600.875.374', 'G07.110'], ['A08.186.211'], ['B01.043.075.189.250.750.550'], ['A16.710'], ['G08.686.784.769'], ['C01.610.752'], ['C01.610.701.688', 'C01.610.752.625', 'C22.674.710'], ['B01.050.150.900.649.313.500.380.791'], ['G01.910.857']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
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| 0
| 0
| 0
| 0
| 0
| 0
|
Rural workers' health and productivity: an economic assessment of pesticide use in Minas Gerais, Brazil.
|
This paper evaluates the economic impact of pesticide intoxication on the health of rural workers in the State of Minas Gerais, Brazil. To estimate the economic impact we considered the purchase cost of pesticides and the treatment costs of rural workers' affected by intoxication. These impacts were compared to the benefits of pesticide use, as estimated by the crop losses avoided through pesticide application. Data were obtained from the Fundacentro/Ministry of Work agency for the years 1991-2000, in nine municipalities of Minas Gerais, Brazil; and included epidemiological surveillance records and blood screening results. Rural workers' probabilities of intoxication were estimated as a function of their expositure characteristics by means of a logistic regression model. The changes in marginal probabilities associated with changes in expositure characteristics were also estimated. When health intoxication costs are included in the farmer's decision process, the financial benefit of pesticide use with certain crops is reduced. For zucchini, beans and corn, respectively, treatment costs represent about 42%, 25% and 25% of the pesticide use benefit. This study points to the need for an extensive investigation into the real benefits of pesticide use and its consequences for the environment and health in Brazil.
|
['Adolescent', 'Adult', "Agricultural Workers' Diseases", 'Brazil', 'Efficiency', 'Employer Health Costs', 'Female', 'Health Status Indicators', 'Humans', 'Male', 'Middle Aged', 'Occupational Exposure', 'Pesticides', 'Risk Factors', 'Rural Health']
| 14,619,267
|
[['M01.060.057'], ['M01.060.116'], ['C24.080'], ['Z01.107.757.176'], ['F02.784.692.351', 'N04.452.209'], ['N03.219.151.400.375', 'N05.300.375.350'], ['E05.318.308.980.438.475', 'N05.715.360.300.800.438.375', 'N06.850.520.308.980.438.475'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['N06.850.460.350.600'], ['D27.720.031.700', 'D27.888.723'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['N01.400.548.750']]
|
['Named Groups [M]', 'Diseases [C]', 'Geographicals [Z]', 'Psychiatry and Psychology [F]', 'Health Care [N]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Chemicals and Drugs [D]']
| 0
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Activity of glutathione-dependent enzymes in long term diabetes. I. Activity of glutathione S-transferase and glutathione peroxidase in the liver of alloxan induced diabetic rats.
|
Cytosolic liver glutathione S-transferase (GST) activity was decreased for CDNB and DCNB as substrates in long term alloxan induced diabetes. Similar to cytosolic, microsomal glutathione S-transferase activity was also decreased for CDNB. In contrast, both microsomal and cytosolic GST activities for ETA as well as cytosolic and microsomal glutathione (GSH) contents were unaffected. The activity of Se-dependent glutathione peroxidase activity, but not nonSe-dependent peroxidase activity was increased in diabetic rats. The results suggest that diabetic state has a different effect on each isoenzyme of hepatic glutathione S-transferase activity. After insulin treatment of diabetic animals the activities of both cytosolic and microsomal GST was not restored and the activity of non Se-GSHPx was significantly lower than the control value.
|
['Alloxan', 'Animals', 'Cytosol', 'Diabetes Mellitus, Experimental', 'Glutathione', 'Glutathione Peroxidase', 'Glutathione Transferase', 'Liver', 'Male', 'Microsomes, Liver', 'Rats']
| 8,960,250
|
[['D03.383.742.698.122'], ['B01.050'], ['A11.284.430.214.200', 'A11.284.430.429.200', 'A11.284.835.450.200'], ['C18.452.394.750.074', 'C19.246.240', 'E05.598.500.374'], ['D12.644.456.448'], ['D08.811.682.732.500'], ['D08.811.913.225.500'], ['A03.620'], ['A11.284.835.540.541'], ['B01.050.150.900.649.313.992.635.505.700']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Anatomy [A]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Our experiences with measurement of new potential biomarkers in the diagnosis of latent forms of myocardial ischemia.
|
BACKGROUND: The need for a laboratory marker of myocardial ischemia has been alluded to for at least the last decade.AIM: The aim of this study was to evaluate the diagnostic importance of the myosin light chain-1 (MLC-1), clusterin and Reg-Ialpha in patients with suspected myocardial ischemia.METHODS: A group of 176 at high-risk for myocardial ischemia subjects was evaluated and divided into two subgroups using myocardial SPECT (Single Photon Emission Computed Tomography) - individuals with and without signs of myocardial ischemia. Laboratory markers in venous blood were repeatedly examined in all subjects: a) immediately prior to SPECT: C-reactive protein, Haemoglobin, Hematocrite, Lactate, MLC-1, Clusterin, Reg-Ialpha b) at subjective maximum: Hb, Htc, lactate, MLC-1, Clusterin, Reg-Ialpha c) 30 min after stress levels reached their peak: MLC-1, Clusterin, Reg-Ialpha and d) 60 min after peak stress levels: MLC-1, Clusterin, Reg-Ialpha.RESULTS: Patients were divided into subgroups according to their positive and negative SPECT results (positive: n = 37; negative: n = 139). MLC-1 values were different for all 4 blood collections. An increase in MLC-1 > 2.2 mg/l showed 64 % sensitivity and 88 % specificity for the diagnosed presence of myocardial ischemia (AUC 0.81; LR+ 5.9; PPV+ 68 % and NPV- 87 %). There was no significant difference between the groups in terms of Clusterin and Reg-Ialpha for any of the sampling periods.CONCLUSIONS: High diagnostic efficacy of detectable MLC-1 was shown for the diagnosis of latent myocardial ischemia. Measurement of serum Clusterin or Reg-Ialpha did not sufficient for the diagnosis of latent myocardial ischemia.
|
['Biomarkers', 'Clusterin', 'Female', 'Humans', 'Lithostathine', 'Male', 'Myocardial Ischemia', 'Myosin Light Chains', 'Sensitivity and Specificity', 'Tomography, Emission-Computed, Single-Photon']
| 17,426,785
|
[['D23.101'], ['D12.776.395.207', 'D12.776.580.215'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.776.503.280.578.500'], ['C14.280.647', 'C14.907.585'], ['D05.750.078.730.475.200', 'D12.776.157.125.475', 'D12.776.210.500.600.200', 'D12.776.220.525.475.200'], ['E05.318.370.800', 'E05.318.740.872', 'G17.800', 'N05.715.360.325.700', 'N05.715.360.750.725', 'N06.850.520.445.800', 'N06.850.520.830.872'], ['E01.370.350.350.800.800', 'E01.370.350.600.350.800.800', 'E01.370.350.710.800.800', 'E01.370.350.825.800.800', 'E01.370.384.730.800.800']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
[Diagnosis and treatment for solitary necrotic nodule of the liver: report of 15 patients].
|
OBJECTIVE: To investigate the diagnostic and therapeutic approach of solitary necrotic nodule of the liver (SNNL).METHODS: Fifteen cases were diagnosed as SNNL from June 1999 to December 2005. The clinical characteristics, imaging findings, diagnosis and treatment were analyzed with related literature retrospectively.RESULTS: The patients manifested abdominal pain and discomfort in 7 cases (46.7%), fever in 1 case (6.7%), debilitation in 1 case (6.7%). Lesions were screened as hypoechogenic patterns in B ultrasound, and CT scan confirmed that the lesion appeared slightly hypodense compared with the normal liver parenchyma without detectable enhanced graphic phases. No significant enhancement was on dynamic magnetic resonance imaging study. All of the nodules demonstrated hypointense and isointense signal relative to parenchyma of liver on both T1 and T2-weighted images. Histologically, the lesion composed mainly of coagulative necrosis with a homogeneous periphery, and the central zone had a rough patchy appearance with cellular debris. The coagulative necrosis was surrounded by a thin boundary of collagen fibers with scanty mononuclear, lymphocyte, plasmocyte inflammatory cells and elastic fibers. Preoperative laboratory examinations showed hepatic function slightly abnormal in 3 patients, and AFP level was normal in all patients. Diagnosis of SNNL was established in 4 cases (26.7%) preoperatively. All patients underwent liver resection with no recurrence within 3 months to 6 years follow-up.CONCLUSIONS: Preoperative diagnosis of SNNL can be established via comprehensive analysis of clinical characteristics and imaging findings. Liver resection is the optimal therapeutic approach.
|
['Adult', 'Aged', 'Female', 'Follow-Up Studies', 'Humans', 'Liver', 'Liver Neoplasms', 'Male', 'Middle Aged', 'Necrosis', 'Retrospective Studies']
| 18,241,567
|
[['M01.060.116'], ['M01.060.116.100'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A03.620'], ['C04.588.274.623', 'C06.301.623', 'C06.552.697'], ['M01.060.116.630'], ['C23.550.717'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Anatomy [A]', 'Diseases [C]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Comparison of long-term outcome after percutaneous coronary intervention versus coronary artery bypass grafting in patients with unprotected left main coronary artery disease (from the CREDO-Kyoto PCI/CABG Registry Cohort-2).
|
The long-term outcome of percutaneous coronary intervention (PCI) compared to coronary artery bypass grafting (CABG) for unprotected left main coronary artery disease (ULMCAD) remains to be investigated. We identified 1,005 patients with ULMCAD of 15,939 patients with first coronary revascularization enrolled in the CREDO-Kyoto PCI/CABG Registry Cohort-2. Cumulative 3-year incidence of a composite of death/myocardial infarction (MI)/stroke was significantly higher in the PCI group than in the CABG group (22.7% vs 14.8%, p = 0.0006, log-rank test). However, the adjusted outcome was not different between the PCI and CABG groups (hazard ratio [HR] 1.30, 95% confidence interval [CI] 0.79 to 2.15, p = 0.30). Stratified analysis using the SYNTAX score demonstrated that risk for a composite of death/MI/stroke was not different between the 2 treatment groups in patients with low (<23) and intermediate (23 to 33) SYNTAX scores (adjusted HR 1.70, 95% CI 0.77 to 3.76, p = 0.19; adjusted HR 0.86, 95% CI 0.37 to 1.99, p = 0.72, respectively), whereas in patients with a high SYNTAX score (?33), it was significantly higher after PCI than after CABG (adjusted HR 2.61, 95% CI 1.32 to 5.16, p = 0.006). In conclusion, risk of PCI for serious adverse events seemed to be comparable to that after CABG in patients with ULMCAD with a low or intermediate SYNTAX score, whereas PCI compared with CABG was associated with a higher risk for serious adverse events in patients with a high SYNTAX score.
|
['Aged', 'Angioplasty, Balloon, Coronary', 'Coronary Angiography', 'Coronary Artery Bypass', 'Coronary Artery Disease', 'Coronary Vessels', 'Female', 'Follow-Up Studies', 'Humans', 'Incidence', 'Japan', 'Male', 'Myocardial Infarction', 'Postoperative Complications', 'Registries', 'Retrospective Studies', 'Risk Factors', 'Stroke', 'Survival Rate', 'Time Factors']
| 22,721,575
|
[['M01.060.116.100'], ['E02.148.050.060.100', 'E04.100.376.719.100', 'E04.100.814.529.124.060.100', 'E04.100.814.529.968.050', 'E04.502.382.124.060.100', 'E04.502.382.968.050', 'E04.928.220.520.100', 'E05.157.016.060.100'], ['E01.370.350.130.625', 'E01.370.350.700.060.200', 'E01.370.370.050.200', 'E01.370.370.380.200'], ['E04.100.376.719.332', 'E04.100.814.868.750', 'E04.928.220.520.220'], ['C14.280.647.250.260', 'C14.907.137.126.339', 'C14.907.585.250.260'], ['A07.015.114.269', 'A07.015.908.194'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.308.985.525.375', 'N01.224.935.597.500', 'N06.850.505.400.975.525.375', 'N06.850.520.308.985.525.375'], ['Z01.252.474.463', 'Z01.639.595'], ['C14.280.647.500', 'C14.907.585.500', 'C23.550.513.355.750', 'C23.550.717.489.750'], ['C23.550.767'], ['E05.318.308.970', 'N04.452.859.819', 'N05.715.360.300.715.700', 'N06.850.520.308.970'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['C10.228.140.300.775', 'C14.907.253.855'], ['E05.318.308.985.550.900', 'N01.224.935.698.826', 'N06.850.505.400.975.550.900', 'N06.850.520.308.985.550.900'], ['G01.910.857']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Anatomy [A]', 'Health Care [N]', 'Organisms [B]', 'Geographicals [Z]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Minimizing Ports During Robotic Partial Nephrectomy.
|
BACKGROUND AND OBJECTIVE: Robotic upper urinary tract surgery is in most of the cases performed utilizing a standard 5 port configuration. Fewer ports can potentially produce a less invasive operation. Taking in consideration the above we report a novel technique for robot assisted laparoscopic partial nephrectomy utilizing fewer ports and we test its feasibility and safety profile.METHODS: Data on 11 robot-assisted laparoscopic partial nephrectomies performed by using our technique from February 2015 through June 2015 were retrospectively analyzed. The robotic platform used was DaVinci Xi (Intuitive Surgical, Inc., Sunnyvale, California, USA) with a 3-arm setup. The AirSeal system (SurgiQuest, Milford, Connecticut, USA) was used as a port allowing simultaneous introduction of 2 instruments for the bedside surgeon, obviating the need for an additional (fourth) robotic arm. A long suction-and-irrigation device and atraumatic grasping forceps were used. Both instruments were introduced through the trocar of the AirSeal system, making simultaneous introduction and use possible. We preferred the long suction-and-irrigation device, because it minimizes collision of the instruments.RESULTS: Mean age and BMI of the patients were 55 ±14.6 y and 29.18 ± 6.85, respectively. Seven tumors were on the right side and 4 were on the left. The mean size of the tumors was 32.45 mm (± 11.31). Surgical time was 132.2 minutes (±37.17), with an estimated blood loss and ischemia time of 103.63 mL (±65.92) and 16.72 minutes (±9.52), respectively. One patient had postoperative bleeding that was resolved without transfusion. The median hospitalization period was 3.9 d (±0.53). Loss of intra-abdominal pressure was not observed, and pressure was stable at 10 mm Hg.CONCLUSION: The AirSeal System and its valveless trocar eliminated the need for an additional port placement in our series. The technique is feasible, safe, and reproducible; therefore, it may be implemented in selected cases of robot-assisted partial nephrectomies.
|
['Adult', 'Aged', 'Blood Loss, Surgical', 'Equipment Design', 'Female', 'Humans', 'Kidney Neoplasms', 'Male', 'Middle Aged', 'Minimally Invasive Surgical Procedures', 'Nephrectomy', 'Operative Time', 'Retrospective Studies', 'Robotic Surgical Procedures']
| 27,403,042
|
[['M01.060.116'], ['M01.060.116.100'], ['C23.550.414.300', 'C23.550.505.300'], ['E05.320'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C04.588.945.947.535', 'C12.758.820.750', 'C12.777.419.473', 'C13.351.937.820.535', 'C13.351.968.419.473'], ['M01.060.116.630'], ['E04.502'], ['E04.950.774.435'], ['E04.614.374.500', 'N02.421.585.753.374.500'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E04.749.500', 'J01.897.104.834.500']]
|
['Named Groups [M]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Health Care [N]', 'Technology, Industry, and Agriculture [J]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 1
| 1
| 0
|
Three-month-old infants show enhanced behavioral and neural sensitivity to fearful faces.
|
An important feature of the development of emotion recognition in infants is the emergence of a robust attentional bias for fearful faces. There is some debate about when this enhanced sensitivity to fearful expressions develops. The current study explored whether 3-month-olds demonstrate differential behavioral and neural responding to happy and fearful faces. Three-month-old infants (n = 69) participated in a behavioral task that assessed whether they show a visual preference for fearful faces and an event-related potential (ERP) task that assessed their neural responses to fearful and happy faces. Infants showed a looking preference for fearful over happy faces. They also showed differential neural responding over occiptotemporal regions that have been implicated in face perception (i.e., N290, P400), but not over frontocentral regions that have been implicated in attentional processes (i.e., Nc). These findings suggest that 3-month-olds display an early perceptual sensitivity to fearful faces, which may presage the emergence of the attentional bias for fearful faces in older infants. Tracking the ontogeny of this phenomenon is necessary to understand its relationship with later developmental outcomes.
|
['Emotions', 'Facial Expression', 'Fear', 'Female', 'Humans', 'Infant', 'Male']
| 32,072,932
|
[['F01.470'], ['E01.370.600.225', 'F01.145.209.530.385'], ['F01.470.361'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703']]
|
['Psychiatry and Psychology [F]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Named Groups [M]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Fetal middle cerebral artery Doppler indices and clinical application at Korle Bu Teaching Hospital, Accra, Ghana.
|
OBJECTIVE: To determine normal ranges for various Doppler flow velocity indices of the fetal middle cerebral artery (MCA) and their trends in normal pregnancies at Korle Bu Teaching Hospital, Accra, Ghana.METHODS: A prospective cross-sectional study was conducted at Korle Bu Teaching Hospital in 2015. Included women had a singleton pregnancy of 20-40weeks' duration, dated using an early ultrasonography scan, and normal fetal growth. Interviews were conducted to collect data on sociodemographic characteristics, followed by Doppler ultrasonography of the MCA. The resistive index, pulsatility index, systolic-to-diastolic ratio, and peak systolic velocity of the MCA were determined for all participants.RESULTS: Overall, 458 pregnant women were recruited. The peak systolic velocity increased with advancing gestational age and a positive correlation of r=0.725 (P<0.001) was demonstrated between the peak systolic velocity and the gestational age. The resistive index, pulsatility index, and systolic-to-diastolic ratio of the MCA decreased with advancing gestational age in a parabolic pattern.CONCLUSION: The reference curve for the peak systolic velocity increases with gestational age, whereas the other indices decrease in a parabolic pattern.
|
['Adult', 'Blood Flow Velocity', 'Cross-Sectional Studies', 'Female', 'Fetus', 'Gestational Age', 'Ghana', 'Hospitals, Teaching', 'Humans', 'Middle Cerebral Artery', 'Pregnancy', 'Prospective Studies', 'Reference Values', 'Regression Analysis', 'Ultrasonography, Prenatal']
| 27,177,520
|
[['M01.060.116'], ['E01.370.370.130', 'G09.330.380.630.080'], ['E05.318.372.500.875', 'N05.715.360.330.500.875', 'N06.850.520.450.500.875'], ['A16.378'], ['G07.345.500.325.235.968', 'G08.686.320'], ['Z01.058.290.190.320'], ['N02.278.020.300', 'N02.278.421.639'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A07.015.114.228.550'], ['G08.686.784.769'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['E05.978.810'], ['E05.318.740.750', 'N05.715.360.750.695', 'N06.850.520.830.750'], ['E01.370.350.850.865', 'E01.370.378.630.865']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Anatomy [A]', 'Geographicals [Z]', 'Organisms [B]']
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
The genomic footprint of climate adaptation in Chironomus riparius.
|
The gradual heterogeneity of climatic factors poses varying selection pressures across geographic distances that leave signatures of clinal variation in the genome. Separating signatures of clinal adaptation from signatures of other evolutionary forces, such as demographic processes, genetic drift and adaptation, to nonclinal conditions of the immediate local environment is a major challenge. Here, we examine climate adaptation in five natural populations of the harlequin fly Chironomus riparius sampled along a climatic gradient across Europe. Our study integrates experimental data, individual genome resequencing, Pool-Seq data and population genetic modelling. Common-garden experiments revealed significantly different population growth rates at test temperatures corresponding to the population origin along the climate gradient, suggesting thermal adaptation on the phenotypic level. Based on a population genomic analysis, we derived empirical estimates of historical demography and migration. We used an FST outlier approach to infer positive selection across the climate gradient, in combination with an environmental association analysis. In total, we identified 162 candidate genes as genomic basis of climate adaptation. Enriched functions among these candidate genes involved the apoptotic process and molecular response to heat, as well as functions identified in studies of climate adaptation in other insects. Our results show that local climate conditions impose strong selection pressures and lead to genomic adaptation despite strong gene flow. Moreover, these results imply that selection to different climatic conditions seems to converge on a functional level, at least between different insect species.
|
['Acclimatization', 'Adaptation, Physiological', 'Animals', 'Chironomidae', 'Climate', 'Climate Change', 'Ecosystem', 'Europe', 'Genetic Drift', 'Genetics, Population', 'Genomics', 'Selection, Genetic']
| 29,473,242
|
[['G07.025.133', 'G16.012.500.133'], ['G07.025', 'G16.012.500'], ['B01.050'], ['B01.050.500.131.617.720.500.500.750.712.500.750'], ['G16.500.275.071', 'N06.230.300.100.250'], ['G16.500.175.374'], ['G16.500.275.157', 'N06.230.124'], ['Z01.542'], ['G05.045.300', 'G05.330.320'], ['H01.158.273.343.335'], ['H01.158.273.180.350', 'H01.158.273.343.350'], ['G05.783']]
|
['Phenomena and Processes [G]', 'Organisms [B]', 'Health Care [N]', 'Geographicals [Z]', 'Disciplines and Occupations [H]']
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 1
|
1H NMR techniques in studies of transport of paramagnetic ions in multicellular systems.
|
Two different pulse sequences used in 1H NMR spectroscopy termed free induction decay amplitude recovery (FIDAR) and spin-echo recovery (SER) were applied to studies of transport of paramagnetic ions in multicellular systems. The molar relaxivity of several paramagnetic species (Fe3+, Co2+, Ni2+, Cu2+, Mn2+, MnEDTA2-, dextran-magnetite) in water solutions was measured at 32 MHz resonance frequency. Ionic transport was studied using Mn2+ and MnEDTA2- as models for cations and anions, respectively, and plant root tissue as a model of a multicellular system.
|
['Algorithms', 'Biological Transport', 'Cell Membrane', 'Ions', 'Magnetic Resonance Spectroscopy', 'Manganese', 'Plant Cells', 'Water']
| 3,443,285
|
[['G17.035', 'L01.224.050'], ['G03.143'], ['A11.284.149'], ['D01.248.497'], ['E05.196.867.519'], ['D01.268.556.484', 'D01.268.956.374', 'D01.552.544.484'], ['A11.750'], ['D01.045.250.875', 'D01.248.497.158.459.650', 'D01.650.550.925']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Assessment of tissue-engineered islet graft viability by fluorine magnetic resonance spectroscopy.
|
INTRODUCTION: Despite significant progress in the last decade, islet transplantation remains an experimental therapy for a limited number of patients with type 1 diabetes. Tissue-engineered approaches may provide promising alternatives to the current clinical protocol and would benefit greatly from concurrent development of graft quality assessment techniques. This study was designed to evaluate whether viability of tissue-engineered islet grafts can be assessed using fluorine magnetic resonance spectroscopy ((19)F-MRS), by the noninvasive measurement of oxygen partial pressure (pO(2)) and the subsequent calculation of islet oxygen consumption rate (OCR).METHODS: Scaffolds composed of porcine plasma were seeded with human islets and perfluorodecalin. Each graft was covered with the same volume of culture media in a Petri dish. Four scaffolds were seeded with various numbers (0-8000) of islet equivalents (IE) aliquoted from the same preparation. After randomizing run order, grafts were examined by (19)F-MRS at 37°C using a 5T spectrometer and a single-loop surface coil placed underneath. A standard inversion recovery sequence was used to obtain characteristic (19)F spin-lattice relaxation times (T1), which were converted to steady-state average pO(2) estimates using a previously determined linear calibration (R(2) = 1.000). Each condition was assessed using replicate (19)F-MRS measurements (n = 6-8).RESULTS: Grafts exhibited IE dose-dependent increases in T1 and decreases in pO(2) estimates. From the difference between scaffold pO(2) estimates and ambient pO(2), the islet preparation OCR was calculated to be 95 ± 12 (mean ± standard error of the mean) nmol/(min·mg DNA) using theoretical modeling. This value compared well with OCR values measured using established methods for human islet preparations.CONCLUSIONS: (19)F-MRS can be used for noninvasive pre- and possibly posttransplant assessment of tissue-engineered islet graft viability by estimating the amount of viable, oxygen-consuming tissue in a scaffold.
|
['Animals', 'Cell Survival', 'Diabetes Mellitus, Type 1', 'Dose-Response Relationship, Drug', 'Fluorine', 'Graft Survival', 'Humans', 'Islets of Langerhans', 'Islets of Langerhans Transplantation', 'Magnetic Resonance Spectroscopy', 'Models, Theoretical', 'Oxygen', 'Oxygen Consumption', 'Partial Pressure', 'Swine', 'Tissue Engineering', 'Tissue Scaffolds']
| 22,099,762
|
[['B01.050'], ['G04.346'], ['C18.452.394.750.124', 'C19.246.267', 'C20.111.327'], ['G07.690.773.875', 'G07.690.936.500'], ['D01.268.380.300'], ['G12.875.545.340'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A03.734.414', 'A06.300.414'], ['E02.095.147.500.250', 'E04.270.550', 'E04.936.225.375'], ['E05.196.867.519'], ['E05.599'], ['D01.268.185.550', 'D01.362.670'], ['G03.680'], ['G01.374.715.714'], ['B01.050.150.900.649.313.500.880'], ['E05.481.500.311.500', 'J01.293.069.249.500'], ['E07.206.627', 'E07.695.825']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Technology, Industry, and Agriculture [J]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Refinement and psychometric evaluation of the Attitudes Toward Loss of Hearing Questionnaire.
|
OBJECTIVE: To refine and statistically validate the Attitudes Toward Loss of Hearing Questionnaire (ALHQ) so that it will be appropriate for clinical application and to understand some of the personality attributes underlying attitudes toward hearing loss.DESIGN: An American-English version of an ALHQ, originally designed by Brooks (1989), was completed by 226 men; a subset of 80 also completed personality questionnaires. All subjects underwent pure-tone testing and speech audiometry. Factor analysis was used to extract scales from the ALHQ. Reliability analyses using Cronbach's alpha were carried out on each scale. Test-retest reliability was evaluated from questionnaires completed 6 to 18 mo after initial administration. Multiple regression analysis was used to examine the audiometric and personality determinants of attitudes.RESULTS: Five reliable scales were extracted from a 24-question version of the ALHQ: 1) Social and Emotional Impact of Hearing Loss, 2) Acceptance/ Adjustment to Hearing Loss, 3) Perceived Support from Significant Others, 4) Hearing Aid Stigma, and 5) Awareness of Hearing Loss. Audiometric data explained little of the variance in attitude scores; age and other demographic factors did not correlate with attitudes either. The personality traits of extroversion, self-esteem, and anxiety/neuroticism played a larger role in determining attitude.CONCLUSIONS: The ALHQ is psychometrically acceptable and is a potentially useful clinical tool. It is quick and easy to complete and to score and could be used as a basis for counseling and for following attitude change in patients over time.
|
['Adaptation, Psychological', 'Affect', 'Attitude to Health', 'Audiometry, Pure-Tone', 'Correction of Hearing Impairment', 'Hearing Aids', 'Hearing Disorders', 'Humans', 'Male', 'Personal Satisfaction', 'Psychometrics', 'Speech Perception', 'Surveys and Questionnaires']
| 8,979,038
|
[['F01.058'], ['F01.470.047'], ['F01.100.150', 'N05.300.150'], ['E01.370.382.375.060.055'], ['E02.760.169.063.500.200', 'E02.831.200', 'H02.403.680.600.500', 'N02.421.784.377'], ['E07.305.906.500', 'E07.814.458'], ['C09.218.458', 'C10.597.751.418', 'C23.888.592.763.393'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F01.145.677'], ['F04.711.780'], ['F02.463.593.071.875', 'G07.888.125.875'], ['E05.318.308.980', 'N05.715.360.300.800', 'N06.850.520.308.980']]
|
['Psychiatry and Psychology [F]', 'Health Care [N]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Diseases [C]', 'Organisms [B]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
|
Coblation assisted endoscopic juvenile nasopharyngeal angiofibroma resection.
|
To provide additional support for the use of coblation in the surgical treatment of juvenile nasopharyngeal angiofibroma (JNA) tumors. Coblation radiofrequency has been recently described in endoscopic sinus surgery for polyp and tumor resection from the sinuses to the skull base. This is a case series from our institution in which we safely and successfully treated three adolescent boys with JNA using the coblation assisted technique. The first case was the smallest of the cases (Radkowski stage IB) and was embolized pre-operatively. The second and third cases, both larger in size (Radkowski stage IIC and IIB) did not undergo pre-operative embolization. The total surgical times were 105, 160, and 150 min and the estimated blood losses were 150, 400, and 130 mL, respectively. This yielded a blood loss per minute rate of only 1.4, 2.5, and 0.9 mL/min for the respective cases. None of the three patients required post-operative blood transfusion, nasal packing, or hospitalization of greater than one day. Follow-up showed no complications and no recurrence in these patients. Coblation assisted transnasal endoscopic resection of JNA is a feasible technique that can dissect through and debulk JNA tumor, despite its extreme vascularity. The surgery can be performed with minimal morbidity and low intraoperative blood loss, even with non-embolized tumors up to Radkowski IIC. These finding further support complete resection of JNA tumors using minimally invasive coblation assisted techniques.
|
['Ablation Techniques', 'Adolescent', 'Angiofibroma', 'Endoscopy', 'Humans', 'Male', 'Nasopharyngeal Neoplasms', 'Young Adult']
| 22,269,889
|
[['E04.014'], ['M01.060.057'], ['C04.557.645.100'], ['E01.370.388.250', 'E04.502.250'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C04.588.443.665.710.650', 'C07.550.350.650', 'C07.550.745.650', 'C09.647.710.650', 'C09.775.350.650', 'C09.775.549.650'], ['M01.060.116.815']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Named Groups [M]', 'Diseases [C]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Transapical mitral valve implantation after unclipping of a MitraClip: a glimpse into the future and treatment considerations in mitral regurgitation.
|
AIMS: Transcatheter mitral valve implantation (TMVI) is a novel approach that may enable a less invasive effective reduction of mitral regurgitation (MR). A limitation of the MitraClip is that definitive implantation of a clip precludes future therapy with TMVI. The purpose of this paper is to describe contemporary treatment considerations in patients with mitral valve regurgitation.METHODS AND RESULTS: In this report we describe an attempted MitraClip implantation which resulted in no reduction of MR severity. There was a consensus that additional clips would probably not be effective. MitraClip implantation was therefore abandoned and the clip was removed, allowing subsequent successful TMVI with the Tiara™ system three weeks later. Echocardiography revealed secure seating of the prosthesis with good mitral valve function, trivial paravalvular leakage and transvalvular gradient of 3 mmHg. The patient recovered rapidly and was discharged four days post implant.CONCLUSIONS: The clinical approach towards high-risk patients with significant MR may change in the next few years. In selected patients, in whom an initial attempt with MitraClip implantation results in only limited efficacy, the clip may be retrieved during the index procedure to allow subsequent TMVI.
|
['Adult', 'Cardiac Catheterization', 'Echocardiography, Transesophageal', 'Heart Valve Prosthesis Implantation', 'Humans', 'Male', 'Mitral Valve', 'Mitral Valve Insufficiency', 'Patient Selection', 'Prosthesis Design', 'Risk', 'Surgical Instruments', 'Treatment Outcome']
| 27,290,683
|
[['M01.060.116'], ['E01.370.370.380.140', 'E02.148.442', 'E05.157.250'], ['E01.370.350.130.750.235', 'E01.370.350.850.220.235', 'E01.370.370.380.220.235'], ['E04.100.376.485', 'E04.650.410', 'E04.928.220.410'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A07.541.510.507'], ['C14.280.484.461'], ['E05.581.500.653', 'N04.590.731'], ['E05.320.550', 'E07.695.680'], ['E05.318.740.600.800', 'G17.680.750', 'N05.715.360.750.625.700', 'N06.850.520.830.600.800'], ['E07.858.700'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Anatomy [A]', 'Diseases [C]', 'Health Care [N]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Effects of water extract of Hibiscus sabdariffa, Linn (Malvaceae) 'Roselle' on excretion of a diclofenac formulation.
|
The effect of beverages prepared from the dried calyx of the flowers of Hibiscus sabdariffa on the excretion of diclofenac was investigated using a controlled study in healthy human volunteers. A high pressure liquid chromatographic method was used to analyse the 8 h urine samples collected after the administration of diclofenac with 300 mL (equivalent to 8.18 mg anthocyanins) of the beverage administered daily for 3 days. An unpaired two-tailed t-test was used to analyse for significant difference observed in the amount of diclofenac excreted before and after administration of the beverage. There was a reduction in the amount of diclofenac excreted and the wide variability observed in the control with the water beverage of Hibiscus sabdariffa (p < 0.05). There is an increasing need to counsel patients against the use of plant beverages with drugs.
|
['Adult', 'Anti-Inflammatory Agents, Non-Steroidal', 'Beverages', 'Cross-Over Studies', 'Diclofenac', 'Female', 'Flowers', 'Food-Drug Interactions', 'Hibiscus', 'Humans', 'Kidney', 'Male', 'Phytotherapy', 'Plant Extracts']
| 17,094,172
|
[['M01.060.116'], ['D27.505.696.663.850.014.040.500', 'D27.505.954.158.030', 'D27.505.954.329.030'], ['G07.203.100', 'J02.200'], ['E05.318.370.150', 'N05.715.360.325.150', 'N06.850.520.445.150'], ['D02.241.223.601.210'], ['A18.024.249.500'], ['G07.690.773.968.511'], ['B01.650.940.800.575.912.250.859.821.500.288'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A05.810.453'], ['E02.190.755'], ['D20.215.784.500', 'D26.667']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Technology, Industry, and Agriculture [J]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Anatomy [A]', 'Organisms [B]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 1
| 0
|
Peripheral cholangiocarcinoma and clonorchiasis: CT findings.
|
Sixteen patients with peripheral cholangiocarcinoma of the liver were examined with computed tomography (CT). None of the 16 patients presented with jaundice or had documented cirrhosis. On scans obtained both before and after the injection of contrast material, the tumors were depicted as low-attenuation masses in all cases, with wide variations in homogeneity. The tumor margin was irregular in 12 cases, and there was minimal contrast enhancement of the tumor in 14 cases. In 11 (69%) patients, CT demonstrated masses of markedly low attenuation, which corresponded to areas of diffuse microcystic change seen at histologic examination of resected specimens. In ten (63%) patients, the results of stool or intradermal tests for Clonorchis sinensis were positive. In all ten cases of clonorchiasis, mild, diffuse dilatation of the intrahepatic bile ducts was seen in addition to the low-attenuation masses, but there was no dilatation of the extrahepatic biliary tree. In five of the ten patients with clonorchiasis, stippled or aggregated, powderlike areas of high attenuation were seen on precontrast CT scans; at pathologic examination, those areas were found to be mucin. Extrahepatic metastases were demonstrated in ten (63%) patients. Peripheral cholangiocarcinoma should be the primary diagnostic consideration when these characteristic CT findings are detected in a noncirrhotic patient.
|
['Adenoma, Bile Duct', 'Animals', 'Bile Duct Neoplasms', 'Bile Ducts, Intrahepatic', 'Clonorchiasis', 'Clonorchis sinensis', 'Female', 'Humans', 'Liver Diseases, Parasitic', 'Male', 'Middle Aged', 'Tomography, X-Ray Computed']
| 2,843,940
|
[['C04.557.470.035.085'], ['B01.050'], ['C04.588.274.120.250', 'C06.130.120.120', 'C06.130.320.120', 'C06.301.120.250'], ['A03.159.183.158', 'A03.620.150'], ['C01.610.335.865.148'], ['B01.050.500.500.736.715.520.210'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C01.610.518', 'C06.552.664'], ['M01.060.116.630'], ['E01.370.350.350.810', 'E01.370.350.600.350.700.810', 'E01.370.350.700.700.810', 'E01.370.350.700.810.810', 'E01.370.350.825.810.810']]
|
['Diseases [C]', 'Organisms [B]', 'Anatomy [A]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Running economy and effort after cycling: Effect of methodological choices.
|
Prior exercise can negatively affect movement economy of a subsequent task. However, the impact of cycling exercise on the energy cost of subsequent running is difficult to ascertain, possibly because of the use of different methods of calculating economy. We examined the influence of a simulated cycling bout on running physiological cost (running economy, heart rate and ventilation rates) and perceptual responses (ratings of perceived exertion and effort) by comparing two running bouts, performed before and after cycling using different running economy calculation methods. Seventeen competitive male triathletes ran at race pace before and after a simulated Olympic-distance cycling bout. Running economy was calculated as V?O2 (mL?kg-1?min-1), oxygen cost (EO2, mL?kg-1?m-1) and aerobic energy cost (Eaer, J?kg-1?m-1). All measures of running economy and perceptual responses indicated significant alterations imposed by prior cycling. Despite a good level of agreement with minimal bias between calculation methods, differences (p < 0.05) were observed between Eaer and both V?O2 and EO2. The results confirmed that prior cycling increased physiological cost and perceptual responses in a subsequent running bout. It is recommended that Eaer be calculated as a more valid measure of running economy alongside perceptual responses to assist in the identification of individual responses in running economy following cycling.
|
['Adult', 'Bicycling', 'Energy Metabolism', 'Exercise Test', 'Heart Rate', 'Humans', 'Male', 'Oxygen Consumption', 'Perception', 'Physical Exertion', 'Respiratory Rate', 'Running']
| 32,202,206
|
[['M01.060.116'], ['I03.450.642.845.140'], ['G03.295'], ['E01.370.370.380.250', 'E01.370.386.700.250', 'E05.333.250'], ['E01.370.600.875.500', 'G09.330.380.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G03.680'], ['F02.463.593'], ['G11.427.683'], ['E01.370.600.875.875', 'G09.772.705.730'], ['G11.427.410.568.610', 'G11.427.410.698.277.750', 'I03.350.750', 'I03.450.642.845.610']]
|
['Named Groups [M]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Psychiatry and Psychology [F]']
| 0
| 1
| 0
| 0
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
|
miR-143 regulates hexokinase 2 expression in cancer cells.
|
Tumor cells activate pathways that facilitate and stimulate glycolysis even in the presence of adequate levels of oxygen in order to satisfy their continuous need of molecules, such as nucleotides, ATP and fatty acids, necessary to support their rapid proliferation. Accordingly, a variety of human tumors are characterized by elevated expression levels of the hexokinase 2 isoform (HK2). Although different molecular mechanisms, including genetic and epigenetic mechanisms, have been suggested to account for the altered expression of HK2 in tumors, the potential role of microRNAs (miRNAs) in the regulation of HK2 expression has not been evaluated. Here, we report that miR-143 inhibits HK2 expression via a conserved miR-143 recognition motif located in the 3'-untranslated region (3'UTR) of HK2 mRNA. We demonstrate that miR143 inhibits HK2 expression both in primary keratinocytes and in head and neck squamous cell carcinoma (HNSCC)-derived cell lines. Importantly, we found that miR-143 inversely correlates with HK2 expression in HNSCC-derived cell lines and in primary tumors. We also report that the miRNA-dependent regulation of hexokinase expression is not limited to HK2 as miR-138 targets HK1 via a specific recognition motif located in its 3'UTR. All these data unveil a new miRNA-dependent mechanism of regulation of hexokinase expression potentially important in the regulation of glucose metabolism of cancer cells.
|
['Carcinoma, Squamous Cell', 'Cell Line, Tumor', 'Gene Expression Regulation, Neoplastic', 'Head and Neck Neoplasms', 'Hexokinase', 'Humans', 'Keratinocytes', 'MicroRNAs']
| 22,469,988
|
[['C04.557.470.200.400', 'C04.557.470.700.400'], ['A11.251.210.190', 'A11.251.860.180'], ['G05.308.370'], ['C04.588.443'], ['D08.811.913.696.620.300'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A11.409.500', 'A11.436.397'], ['D13.150.650.319', 'D13.444.735.150.319', 'D13.444.735.790.552.500']]
|
['Diseases [C]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Organisms [B]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Sustained attention and response inhibition in young children at risk for Attention Deficit/Hyperactivity Disorder.
|
BACKGROUND: Studies of school-aged children, adolescents, and adults with Attention Deficit/Hyperactivity Disorder (ADHD) have variably shown ADHD-related impairment in both inhibitory control and sustained attention. However, few studies have examined ADHD-associated patterns of performance on these tasks among younger children (below age 7 years).METHODS: A combined continuous performance test and go/no-go task (CPT/GNG) and the Day-Night Stroop Task (DNST) were administered to an ethnically diverse sample of 3.44- to 6.95-year-old children rated by parents and teachers as being either high risk or low risk for ADHD. All children performed the DNST (N = 71) and a subset of the sample (N = 44) performed the CPT/GNG. Analyses assessed task validity as well as the effects of age and risk status.RESULTS: Significant main effects for age and risk status were found on all tasks. In addition, age x condition interactions were found for the CPT and DNST, which suggest that the tasks were sensitive to age-related changes in sustained attention and inhibitory control respectively. No significant risk status x condition interactions were found, suggesting that young children at risk for ADHD do not exhibit specific deficits in either inhibitory control or sustained attention. The most consistent effect related to risk status across tasks was the greater number of errors and longer and more variable reaction times on the part of children at risk for ADHD irrespective of condition.CONCLUSIONS: ADHD-associated decrements in performance on these tasks appear to be attributable either to generalized behavioral dysregulation or poor state regulation rather than to deficient inhibitory control.
|
['Age Factors', 'Attention', 'Attention Deficit Disorder with Hyperactivity', 'Child', 'Child, Preschool', 'Female', 'Humans', 'Inhibition, Psychological', 'Male', 'Psychometrics', 'Reaction Time', 'Risk Factors', 'Task Performance and Analysis', 'Visual Perception']
| 16,238,669
|
[['N05.715.350.075', 'N06.850.490.250'], ['F02.830.104.214'], ['F03.625.094.150'], ['M01.060.406'], ['M01.060.406.448'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F01.145.544', 'F02.463.425.475', 'F02.739.794.405'], ['F04.711.780'], ['E05.796.817', 'F02.830.650', 'F04.669.817', 'G11.561.677'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['F02.784.412.846', 'F02.784.692.746', 'F02.808.600'], ['F02.463.593.932']]
|
['Health Care [N]', 'Psychiatry and Psychology [F]', 'Named Groups [M]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
M-wave of proximal retina in cat.
|
There has been relatively little known about responses from proximal retina in mammals that could contribute to the electroretinogram (ERG). Recently, there has been evidence that the proximal retina is involved in generating the pattern electroretinogram (PERG). In the present work we investigated proximal retinal activity in the intact cat eye during light adaptation. Extracellular potentials evoked in response to circular spots of light, flashed on steady backgrounds, were recorded with microelectrodes placed intraretinally at different depths. Prominent negative responses were found in proximal retina that could be identified as the M-wave previously observed only in cold-blooded retinas. Like the cold-blooded responses, the cat's M-wave consisted of negative-going potentials at stimulus onset and offset that were maximum in amplitude with small spots. By analogy to the cold-blooded data, the cat M-wave is presumed to be the extracellular voltage arising from M?ller cell responses to K+ released by proximal retinal neurons. In addition, the cat M-wave only appeared with backgrounds at and above rod saturation and had short latencies (30 ms) at stimulus onset and offset, indicating that it is a cone-driven response. The M-wave could be clearly distinguished from PII (b-wave and DC component) on the basis of its form, depth distribution, and stimulus-response characteristics. For example, photopic PII had its maximum voltage in the distal retinal at 55% retinal depth, whereas the M-wave was maximal in the proximal retina at 25% retinal depth. Also, PII simply increased in amplitude as stimulus spots were enlarged, whereas the M-wave exhibited spatial tuning. Under light-adapted conditions and with small-spot stimuli the M-wave is the largest extracellular voltage in cat retina. By recording the vitreal ERG near the retinal surface with the microelectrode referenced to a silver wire in the vitreous, we found that the M-wave in response to a small spot always had a negative polarity in the vitreous. Thus, unlike PII, the M-wave does not reverse polarity at the vitreo-retinal border. Because of stray-light effects, however, we were not able to assess the amplitude of the M-wave's contribution to the ERG obtained with diffuse retinal illumination. We conclude that the M-wave is present in the cat as a prominent cone-driven response of proximal retina that is separate from the b-wave, and whose significance for electroretinographic recordings remains to be determined.
|
['Adaptation, Ocular', 'Animals', 'Cats', 'Electroretinography', 'Light', 'Reaction Time', 'Retina']
| 3,783,227
|
[['G14.020'], ['B01.050'], ['B01.050.150.900.649.313.750.377.750.250.125'], ['E01.370.380.225', 'E01.370.405.270'], ['G01.358.500.505.650', 'G01.590.540', 'G01.750.250.650', 'G01.750.770.578'], ['E05.796.817', 'F02.830.650', 'F04.669.817', 'G11.561.677'], ['A09.371.729']]
|
['Phenomena and Processes [G]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Psychiatry and Psychology [F]', 'Anatomy [A]']
| 1
| 1
| 0
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Postoperative necrotizing scleritis: a report of four cases.
|
Postoperative necrotizing scleritis should be considered in cases of persistent localized postoperative inflammation following all forms of surgical trauma. We present the history, clinical findings, and follow-up data of four patients with postoperative necrotizing scleritis. The clinical records of four patients who developed scleritis following ocular surgery were retrospectively reviewed. The first step in managing necrotizing scleritis is to rule out infectious etiology. Surgically induced necrotizing scleritis is an immune-mediated condition that can coexist with concomitant infectious condition, i.e. endophthalmitis, but response to immunosuppression leads to resolution of the disease and verifies the diagnosis.
|
['Female', 'Glucocorticoids', 'Humans', 'Immunosuppressive Agents', 'Lens Implantation, Intraocular', 'Male', 'Methotrexate', 'Middle Aged', 'Phacoemulsification', 'Postoperative Complications', 'Prednisolone', 'Retrospective Studies', 'Scleritis', 'Trabeculectomy', 'Vitrectomy']
| 25,371,644
|
[['D06.472.040.543', 'D27.505.696.399.472.488'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D27.505.696.477.656'], ['E04.540.825.600'], ['D03.633.100.733.631.192.500'], ['M01.060.116.630'], ['E04.540.825.249.704', 'E04.943.875'], ['C23.550.767'], ['D04.210.500.745.432.769.795'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['C11.790.500'], ['E04.540.450.700'], ['E04.540.960']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Named Groups [M]', 'Diseases [C]', 'Health Care [N]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Cell proliferation in EMT6 tumours treated with single doses of X-rays or hydroxyurea. II. Computer simulations.
|
A computer simulation technique was used to analyse data on the proliferation of clonogenic cells in EMT6 tumours treated with 5 mg/mouse of hydroxyurea (HU) or 3.0 Gy (300 rads) X-rays. This simulation technique is able to determine the respective roles of selective killing, blocks in cell progression and recruitment of the treated population. When the technique was applied to tumours treated with HU, it was possible to prove that both a G1/S block and recruitment occurred. These phenomena could not have been demonstrated quantitatively, or even qualitatively, without the use of the simulation. After irradiation, blocks in cell progression and differences in the proliferative patterns of the surviving clonogenic cells and the total tumour cell population were found.
|
['Animals', 'Cell Division', 'Clone Cells', 'Computers', 'Hydroxyurea', 'Interphase', 'Mice', 'Mitotic Index', 'Neoplasms, Experimental', 'X-Rays']
| 7,371,067
|
[['B01.050'], ['G04.144.220', 'G04.161.750.500', 'G05.113', 'G07.345.249.410.750.500'], ['A11.251.353'], ['L01.224.230.260'], ['D02.065.950.395'], ['G04.144.500'], ['B01.050.150.900.649.313.992.635.505.500'], ['E01.370.225.500.385.500', 'E05.200.500.385.500', 'E05.242.385.500'], ['C04.619', 'E05.598.500.496'], ['G01.358.500.505.970', 'G01.750.250.970', 'G01.750.750.918']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Information Science [L]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Structure/function analysis of the Pantoea stewartii quorum-sensing regulator EsaR as an activator of transcription.
|
In Pantoea stewartii subsp. stewartii, two regulatory proteins are key to the process of cell-cell communication known as quorum sensing: the LuxI and LuxR homologues EsaI and EsaR. Most LuxR homologues function as activators of transcription in the presence of their cognate acylated homoserine lactone (AHL) signal. However, EsaR was initially found to function as a repressor in the absence of AHL. Previous studies demonstrated that, in the absence of AHL, EsaR retains the ability to function as a weak activator of the lux operon in recombinant Escherichia coli. Here it is shown that both the N-terminal and the C-terminal domains of EsaR are necessary for positive regulation. A site-directed mutagenesis study, guided by homology modeling to LuxR and TraR, has revealed three critical residues in EsaR that are involved in activation of RNA polymerase. In addition, a native EsaR-activated promoter has been identified, which controls expression of a putative regulatory sRNA in P. stewartii.
|
['Acyl-Butyrolactones', 'Bacterial Proteins', 'DNA Mutational Analysis', 'DNA-Directed RNA Polymerases', 'Escherichia coli', 'Gene Expression Regulation, Bacterial', 'Mutagenesis, Site-Directed', 'Pantoea', 'Promoter Regions, Genetic', 'Protein Structure, Tertiary', 'Quorum Sensing', 'Recombinant Proteins', 'Sequence Homology, Amino Acid', 'Transcription Factors', 'Transcription, Genetic']
| 19,820,098
|
[['D02.540.232'], ['D12.776.097'], ['E05.393.760.700.300'], ['D08.811.913.696.445.735.270'], ['B03.440.450.425.325.300', 'B03.660.250.150.180.100'], ['G05.308.300'], ['E05.393.420.601.575'], ['B03.440.450.425.580', 'B03.660.250.150.540'], ['G02.111.570.080.689.675', 'G05.360.080.689.675', 'G05.360.340.024.340.137.750.680'], ['G02.111.570.820.709.610'], ['G04.085.700', 'G06.550.700'], ['D12.776.828'], ['G02.111.810.200', 'G05.810.200'], ['D12.776.930'], ['G02.111.873', 'G05.297.700']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
T cells responding to Trypanosoma cruzi detected by membrane TNF-á and CD154 in chagasic patients.
|
INTRODUCTION: Chagas disease is a parasitic infection whose pathogenesis is related to parasite persistence and a dysfunctional cellular immune response. Variability in cytokine secretion among chronic Trypanosoma cruzi-infected patients might preclude the identification of the pool of antigen specific T cells. The goal of this study was to determine the fraction of T cells responding to T. cruzi antigen measured by the expression of membrane TNF-á and CD154.METHODS: A total of 21 chagasic patients, 11 healthy and 5 non-chagasic cardiomyopathy controls were analyzed. PBMCs were short-term cultured in the presence of anti-CD28, anti-CD49d, anti-TNF-á, and TACE (TNF-á converting enzyme) inhibitor either under T. cruzi-lysate or polyclonal stimuli. Cells were stained with anti-CD3, anti-CD4, anti-CD8, and anti-CD154, and analyzed with flow cytometry.RESULTS: CD4+ and CD8+ T cells in chagasic patients displayed higher percentages of membrane-bound TNF-á+ and CD154+ compared with controls after T. cruzi-antigen stimulation. Both markers displayed a positive correlation in the T cell subpopulations analyzed. Symptomatic chagasic patients were differentiated from asymptomatic patients based on the expression of CD154 and membrane TNF-á in TCD4+ and TCD8+ compartments, respectively.CONCLUSIONS: These results show that both markers could be useful for assessing the pool of antigen-specific T cells in chronic chagasic patients.
|
['Adult', 'CD4-Positive T-Lymphocytes', 'CD40 Ligand', 'CD8-Positive T-Lymphocytes', 'Cell Membrane', 'Chagas Disease', 'Female', 'Humans', 'Male', 'Middle Aged', 'Trypanosoma cruzi', 'Tumor Necrosis Factor-alpha']
| 28,967,229
|
[['M01.060.116'], ['A11.118.637.555.567.569.200', 'A15.145.229.637.555.567.569.200', 'A15.382.490.555.567.569.200'], ['D12.644.276.374.750.124', 'D12.776.395.550.185', 'D12.776.467.374.750.124', 'D12.776.543.550.198', 'D23.529.374.750.124'], ['A11.118.637.555.567.569.220', 'A15.145.229.637.555.567.569.220', 'A15.382.490.555.567.569.220'], ['A11.284.149'], ['C01.610.752.300.900.200', 'C01.920.625'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['B01.268.475.868.887.140'], ['D12.644.276.374.500.800', 'D12.644.276.374.750.626', 'D12.776.124.900', 'D12.776.395.930', 'D12.776.467.374.500.800', 'D12.776.467.374.750.626', 'D23.529.374.500.800', 'D23.529.374.750.626']]
|
['Named Groups [M]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Organisms [B]']
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Antimicrobial resistance in faecal Escherichia coli isolates from farmed red deer and wild small mammals. Detection of a multiresistant E. coli producing extended-spectrum beta-lactamase.
|
Eighty-nine Escherichia coli isolates recovered from faeces of red deer and small mammals, cohabiting the same area, were analyzed to determine the prevalence and mechanisms of antimicrobial resistance and molecular typing. Antimicrobial resistance was detected in 6.7% of isolates, with resistances to tetracycline and quinolones being the most common. An E. coli strain carrying blaCTX-M-1 as well as other antibiotic resistant genes included in an unusual class 1 integron (Intl1-dfrA16-blaPSE-1-aadA2-cmlA1-aadA1-qacH-IS440-sul3-orf1-mef(B)Ä-IS26) was isolated from a deer. The blaCTX-M-1 gene was transferred by conjugation and transconjugants also acquired an IncN plasmid. This strain was typed as ST224, which seems to be well adapted to both clinical and environmental settings. The phylogenetic distribution of the 89 strains varied depending on the animal host. This work reveals low antimicrobial resistance levels among faecal E. coli from wild mammals, which reflects a lower selective pressure affecting these bacteria, compared to livestock. However, it is remarkable the detection of a multi-resistant ESBL-E. coli with an integron carrying clinically relevant antibiotic-resistance genes, which can contribute to the dissemination of resistance determinants among different ecosystems.
|
['Animals', 'Animals, Domestic', 'Animals, Wild', 'Anti-Infective Agents', 'Deer', 'Drug Resistance, Multiple', 'Escherichia coli', 'Escherichia coli Infections', 'Feces', 'Integrons', 'Microbial Sensitivity Tests', 'Molecular Typing', 'Phylogeny', 'Prevalence', 'Rodentia', 'Spain', 'beta-Lactamases']
| 27,012,919
|
[['B01.050'], ['B01.050.050.116'], ['B01.050.050.300'], ['D27.505.954.122'], ['B01.050.150.900.649.313.500.380.373'], ['G07.690.773.984.300'], ['B03.440.450.425.325.300', 'B03.660.250.150.180.100'], ['C01.150.252.400.310.330'], ['A12.459'], ['G02.111.570.080.708.330.200.500'], ['E01.370.225.875.595', 'E05.200.875.595', 'E05.337.550.400'], ['E01.370.225.875.150.125.457', 'E05.200.875.150.125.457', 'E05.393.542'], ['G05.697', 'G16.075.605', 'L01.100.697'], ['E05.318.308.985.525.750', 'N01.224.935.597.750', 'N06.850.505.400.975.525.750', 'N06.850.520.308.985.525.750'], ['B01.050.150.900.649.313.992'], ['Z01.542.846'], ['D08.811.277.087.180']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Information Science [L]', 'Health Care [N]', 'Geographicals [Z]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 1
| 1
|
Control of illegal medroxyprogesterone acetate-application in veal calves by residue analysis in adipose tissue using HPLC/RIA methods.
|
Procedures for the determination of medroxyprogesterone acetate (MPA) in adipose tissue collected at time of slaughter allowing the control of MPA application to veal calves are described. Screening radioimmunoassays after sample clean-up were sufficient for a first survey of MPA treatments in livestock herds. Validation of all positive samples was performed by two-dimensional (silica gel diol phase and RP-18 phase) HPLC/RIA immunograms. Megestrol acetate and melengestrol acetate with cross-reactivities of 31% and 0.3% respectively were clearly separated by the RP HPLC. With an absolute detection limit of 4 pg MPA/tube (90% relative binding) negative control samples did not exceed 6 pg/tube, equivalent to 6 pg/g fat in the validating method. Seventeen days after intramuscular (i.m.) injection of 24 mg MPA only 32 pg MPA/g fat were found, while i.m. injection of 60 mg MPA and a waiting period of 19 days resulted in 2700 pg MPA/g fat. After feeding two calves 20 micrograms MPA per head daily for 1 week followed by 200 micrograms MPA per head daily for 2 weeks 359 and 468 pg MPA/g fat were measured. In plasma as well as in adipose tissue more than 80% of the whole immunoreactive material was MPA itself, without indications for the presence of cross-reacting MPA metabolites as confirmed by HPLC/RIA immunograms. Based on day of slaughter ratios of accumulation of MPA from plasma into fat of MPA-fed veal calves were 52 and 72 respectively. In urine MPA was only detectable a few days after injection; as compared to a plasma concentration of 950 pg MPA/ml the amount in urine was only 37 pg MPA/ml and also 325 pg unidentified MPA-equivalents/ml.
|
['Adipose Tissue', 'Animals', 'Cattle', 'Chromatography, High Pressure Liquid', 'Drug and Narcotic Control', 'Female', 'Kinetics', 'Male', 'Medroxyprogesterone', 'Medroxyprogesterone Acetate', 'Radioimmunoassay']
| 2,521,473
|
[['A10.165.114'], ['B01.050'], ['B01.050.150.900.649.313.500.380.271'], ['E05.196.181.400.300'], ['I01.880.604.605.250', 'N03.706.615.402.250', 'N04.452.706.310'], ['G01.374.661', 'G02.111.490'], ['D04.210.500.745.745.654.829.395.700'], ['D04.210.500.745.745.654.829.395.700.500'], ['E01.370.384.700', 'E05.478.566.639', 'E05.601.470.639']]
|
['Anatomy [A]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
|
Novel acidophilic â-galactosidase with high activity at extremely acidic pH region from Teratosphaeria acidotherma AIU BGA-1.
|
A â-galactosidase exhibiting maximal activity at pH 1.0 was purified from Teratosphaeria acidotherma AIU BGA-1. The enzyme had a molecular mass of 180 kDa and consisted of two heterosubunits of 120 kDa and 66 kDa. The N-terminal amino acid sequence of the large subunit was found to be SPNLQDIVTVDGESY. These physicochemical properties differed from those of other microbial â-galactosidases. At pH values of 1.5 and pH 4.5, the enzyme exhibited its highest activity at temperatures of 70°C and 80°C, respectively. Thus, the enzyme exhibited the lowest optimal pH and highest optimal temperature among the microbial â-galactosidases thus reported. The enzyme retained more than 80% of its original activity in the pH range from 2.0 to 8.0 by incubation at 50°C for 30 min. The enzyme hydrolyzed 4-nitrophenyl-â-D-fucopyranoside, 2-nitrophenyl-â-D-galactopyranoside, and 4-nitrophenyl-â-D-galacto-pyranoside at relative reaction rates of 100, 59, and 24, respectively, at pH 1.5, and its affinity for â-D-galactopyranosides was higher than that for â-D-fucopyranosides. The enzyme also efficiently hydrolyzed lactose in milk and whey from yoghurt at pH 1.5.
|
['Amino Acid Sequence', 'Ascomycota', 'Fucose', 'Galactose', 'Hydrogen-Ion Concentration', 'Kinetics', 'Lactase', 'Lactose', 'Molecular Sequence Data', 'Molecular Weight', 'Protein Subunits', 'Substrate Specificity', 'Temperature', 'Whey', 'beta-Galactosidase']
| 25,797,715
|
[['G02.111.570.060', 'L01.453.245.667.060'], ['B01.300.107'], ['D09.254.488'], ['D09.947.875.359.377'], ['G02.300'], ['G01.374.661', 'G02.111.490'], ['D08.811.277.450.410.100.500'], ['D09.698.629.305.340', 'D09.947.750.340'], ['L01.453.245.667'], ['G02.494'], ['D12.776.813'], ['G02.111.835'], ['G01.906.595', 'G16.500.275.063.725.710', 'G16.500.750.775.710', 'N06.230.150.450', 'N06.230.300.100.725.710'], ['A12.790.760', 'G07.203.100.700.750', 'G07.203.300.350.525.760', 'J02.200.700.750', 'J02.500.350.525.760'], ['D08.811.277.450.410.100']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Health Care [N]', 'Anatomy [A]', 'Technology, Industry, and Agriculture [J]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 1
| 0
|
Broad-spectrum monoclonal antibody and a sensitive multi-residue indirect competitive enzyme-linked immunosorbent assay for the antibacterial synergists in samples of animal origin.
|
To monitor the abuse of antibacterial synergists, a hapten, trimethoprim carboxylic derivative (TMPCOOH), was designed by using molecular modelling technology. A broad-spectrum monoclonal antibody (mAb) TMP/2G1 was prepared, for which the IC50 values of trimethoprim, diaveridine, aditoprim, baquiloprim, ormetoprim, and brodimoprim were 0.232, 0.527, 1.479, 4.354, 0.965, and 0.119 µg L-1, respectively. Based on the broad spectrum mAb, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was developed to determine the residues of antibacterial synergists. The limit of detection regarding the developed ic-ELISA for antibacterial synergists ranged from 0.025 to 1.126 µg L-1 in milk, honey and edible animal tissues. The recoveries ranged from 81.4% to 107.7%, with a coefficient of variation less than 20%. A good correlation (R2 = 0.994) between the ic-ELISA and HPLC-MS/MS showed the reliability of the developed ic-ELISA.
|
['Animals', 'Anti-Bacterial Agents', 'Antibodies, Monoclonal', 'Enzyme-Linked Immunosorbent Assay', 'Haptens', 'Honey', 'Limit of Detection', 'Meat', 'Milk', 'Pyrimidines', 'Trimethoprim']
| 30,642,487
|
[['B01.050'], ['D27.505.954.122.085'], ['D12.776.124.486.485.114.224', 'D12.776.124.790.651.114.224', 'D12.776.377.715.548.114.224'], ['E05.478.566.350.170', 'E05.478.566.380.360', 'E05.478.583.400.170', 'E05.601.470.350.170', 'E05.601.470.380.360'], ['D23.050.550.480'], ['G07.203.300.581', 'J02.500.581'], ['E05.318.740.872.374', 'N05.715.360.750.725.500', 'N06.850.520.830.872.500'], ['G07.203.300.600', 'J02.500.600'], ['A12.200.455', 'A12.790', 'G07.203.100.700', 'G07.203.300.350.525', 'J02.200.700', 'J02.500.350.525'], ['D03.383.742'], ['D03.383.742.906']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Technology, Industry, and Agriculture [J]', 'Health Care [N]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
|
Recovery of high purity zinc from filter ash produced during the thermal treatment of waste and inerting of residual materials.
|
The method described below recovers zinc, a valuable metal that is present in high concentrations in filter ash from the thermal treatment of waste, and returns the filter ash stripped of heavy metals to the combustion process in order to destroy organic substances. On an industrial scale, the heavy metals in the filter ash were mobilized by means of hydrochloric acid in the acidic fluids produced in the flue-gas scrubbing process without the addition of further chemicals. A pilot plant for implementing the selective reactive extraction (SRE) method on the ash extracts, using a highly selective complexant, was operated over a period of several months in order to obtain a concentrated, high-purity zinc salt solution (mono metal solution). A zinc depletion rate of 99.8% in the aqueous extract was achieved using mixer-settler units. The residual zinc concentration in the waste water was then < 2 mg L(-1). By stripping the loaded organic phase, a concentrated, high-purity mono metal solution with 190 g L(-1) zinc was obtained. Zinc metal with a purity > 99.99% is then separated by means of electrolysis. To destroy organic substances present in the filter ash, particularly dioxins and furans, the extracted filter ash cake was returned to the combustion process together with household waste. Plant operation, raw and pure gas parameters, and quality of the bottom ash produced were not impacted by such recirculation. The profitability of the overall process is attributable both to the recovery of valuable zinc metal and to the cost savings made in waste water treatment and in the disposal of the waste combustion residues because the remaining mixture of filter ash and bottom ash can be reused in a combined form. This method therefore supports the sustainable and economically viable reuse of filter ash.
|
['Conservation of Natural Resources', 'Electrolysis', 'Filtration', 'Hydrogen-Ion Concentration', 'Industrial Waste', 'Switzerland', 'Temperature', 'Waste Management', 'Water Pollutants, Chemical', 'Zinc']
| 18,229,749
|
[['J01.256', 'N06.230.080'], ['E05.301.250'], ['E05.196.454', 'G01.280', 'G02.263'], ['G02.300'], ['D20.944.420', 'N06.850.460.710.420'], ['Z01.542.883'], ['G01.906.595', 'G16.500.275.063.725.710', 'G16.500.750.775.710', 'N06.230.150.450', 'N06.230.300.100.725.710'], ['N06.850.780.200.800.800.900', 'N06.850.860.510.900'], ['D27.888.284.903.655'], ['D01.268.556.940', 'D01.268.956.906', 'D01.552.544.940']]
|
['Technology, Industry, and Agriculture [J]', 'Health Care [N]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Geographicals [Z]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
|
Smooth muscle hyperplasia in an asthmatic patient: do we know it all?
|
Bronchial smooth muscle hyperplasia is a well-known structural change in asthma. The degree of hyperplasia is related to asthma severity. We report a case of extreme smooth muscle hyperplasia in an asthmatic patient. A 54-year-old female with a diagnosis of analgesic-induced asthma was admitted to our center for nasal polyposis surgery. During her preoperative evaluation, atelectasis of the right middle lobe was detected on chest X-ray. Bronchoscopy revealed the presence of a vegetating polypoid mass obliterating the entrance of the right middle lobe. Histopathological examination of the surgically excised polypoid mass showed benign smooth muscle proliferation with diffuse eosinophilic infiltration. This is a rare case representing an extreme example of benign smooth muscle hyperplasia forming an endobronchial mass in an asthmatic patient.
|
['Asthma', 'Bronchi', 'Female', 'Humans', 'Hyperplasia', 'Middle Aged', 'Muscle, Smooth']
| 20,664,253
|
[['C08.127.108', 'C08.381.495.108', 'C08.674.095', 'C20.543.480.680.095'], ['A04.411.125'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C23.550.444'], ['M01.060.116.630'], ['A02.633.570', 'A10.690.467']]
|
['Diseases [C]', 'Anatomy [A]', 'Organisms [B]', 'Named Groups [M]']
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Pyran Rings Containing Polyketides from Penicillium raistrickii.
|
Five new pyran rings containing polyketides, penicipyrans A-E (1-5), together with the known pestapyrone A (6), were isolated from the saline soil-derived Penicillium raistrickii. Their structures were determined by interpretation of NMR and HRESIMS data. The absolute configurations of compounds 4 and 5 were established by the modified Mosher's method and single-crystal X-ray diffraction analysis, respectively. These compounds possessed high structural diversity including two á-pyrones (1, 2), three isocoumarins (3, 4, 6), and one dihydropyran derivative (5). Among them, Compound 5 exhibited cytotoxicity against HL-60 and K562 cell lines with IC50 values of 4.4 and 8.5 ìM, respectively.
|
['Cell Line, Tumor', 'Crystallography, X-Ray', 'Drug Screening Assays, Antitumor', 'HL-60 Cells', 'Humans', 'Isocoumarins', 'K562 Cells', 'Molecular Structure', 'Nuclear Magnetic Resonance, Biomolecular', 'Penicillium', 'Polyketides', 'Pyrans', 'Pyrones', 'X-Ray Diffraction']
| 28,025,533
|
[['A11.251.210.190', 'A11.251.860.180'], ['E05.196.309.742.225'], ['E01.370.225.500.388', 'E05.200.500.388', 'E05.242.417', 'E05.337.550.200'], ['A11.251.210.190.465', 'A11.251.860.180.465', 'A11.627.340.360.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D03.383.663.283.446.800', 'D03.633.100.150.446.800'], ['A11.251.210.190.510', 'A11.251.860.180.510', 'A11.443.240.497.480'], ['G02.111.570', 'G02.466'], ['E05.196.867.519.550'], ['B01.300.381.662'], ['D02.540.576', 'D04.345.674'], ['D03.383.663'], ['D03.383.663.718'], ['E05.196.309.742', 'E05.196.822.950', 'G01.867.950', 'G02.965']]
|
['Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Detection of Fukushima Daiichi nuclear power plant accident radioactive traces in Monaco.
|
Daily air monitoring of radionuclides in the Principality of Monaco (43°73'N, 7°43'E) after the Fukushima Daiichi nuclear power plant accident showed that only Iodine-131 ((131)I) and Caesium isotopes ((134)Cs and (137)Cs) were detected. The peak of (131)I varied and reached its maximum between March 29th and April 5th, meanwhile both peaks of (134)Cs and (137)Cs arrived later and attained a maximum between April 1st and 4th. Their maximum activity concentrations in air were 354, 30, and 37 ìBq m(-3) respectively. The (134)Cs to (137)Cs activity ratio was close to 1, which is different from that one observed after the Chernobyl accident (around 0.54). Up to 95% of caesium isotopes were washed out by wet scavenging during 27-28th of March, where the maximum deposition rates of (134)Cs and (137)Cs (13.7 and 19.1 mBq m(-2) day(-1), respectively) were observed. The significant input of (134)Cs and (137)Cs into the Mediterranean seawater column (30 m depth) was detected later, on the 24th of May. Radioisotopes of caesium and iodine were found far above the applied detection limits, but still with no concern for harmful radiation exposure and public health. The contamination gradually decreased in air and activity concentrations returned to background values after one or two months.
|
['Air Pollutants, Radioactive', 'Cesium Radioisotopes', 'Fukushima Nuclear Accident', 'Iodine Radioisotopes', 'Japan', 'Monaco', 'Radiation Monitoring', 'Seawater', 'Water Pollutants, Radioactive']
| 22,381,471
|
[['D20.693.101', 'D27.888.284.101.393'], ['D01.268.549.125.500.300', 'D01.268.556.165.500.300', 'D01.496.180.300', 'D01.496.749.190', 'D01.552.528.160.500.300', 'D01.552.544.165.500.300'], ['K01.400.504.984.186', 'N06.850.135.848.750'], ['D01.268.380.400.500.496', 'D01.496.448.496', 'D01.496.749.474'], ['Z01.252.474.463', 'Z01.639.595'], ['Z01.542.616'], ['E05.799.638', 'N06.850.780.375.700', 'N06.850.810.370'], ['G16.500.275.725.500'], ['D20.693.903', 'D27.888.284.903.821']]
|
['Chemicals and Drugs [D]', 'Humanities [K]', 'Health Care [N]', 'Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
|
Characterization of non-adrenergic, non-cholinergic inhibitory responses of the isolated guinea-pig trachea: differences between pre- and post-ganglionic nerve stimulation.
|
1 Differences in the mechanism of non-adrenergic, non-cholinergic (NANC) inhibitory responses to preganglionic- and post-ganglionic nerve stimulation were investigated in the guinea-pig isolated trachea. 2 Stimulation of the vagus nerve at frequencies above 4 Hz elicited NANC relaxation of the trachealis muscle. Responses to low frequencies of stimulation (4-8 Hz) were abolished by the nitric oxide (NO) synthase inhibitor L-NOARG (10 microM), while a L-NOARG resistant component was observed at higher stimulus frequencies. The L-NOARG-resistant component of NANC inhibitory responses to higher frequencies of vagus nerve stimulation were significantly attenuated by the proteinase alpha-chymotrypsin (2 U/ml), suggesting that a neuropeptide such as VIP may contribute to NANC responses. 3 When postganglionic nerves were stimulated by electrical field stimulation (EFS), responses were readily elicited at frequencies below 4 Hz. Like responses to vagus nerve stimulation, responses to low frequency (<4 Hz) EFS were abolished by L-NOARG while a L-NOARG-resistant component was apparent at higher stimulus frequencies. 4 The L-NOARG-resistant component of NANC inhibitory responses to EFS was sensitive to alpha-chymotrypsin only if stimuli were delivered in either long trains at a low frequency (4 Hz for 10-30 s) or short trains of high frequency (16 Hz for 2.5-7.5 s). 5 Responses to preganglionic nerve stimulation were approximately 35% of the amplitude of responses to EFS in the same preparations. 6 In conclusion, responses to preganglionic and postganglionic NANC inhibitory nerve stimulation in the guinea-pig trachea differ in maximum amplitude, frequency-response characteristics and the contributions of cotransmitters. We suggest that these differences may be explained by filtering of preganglionic input to postganglionic NANC neurons. These results have implications in all studies where EFS is considered to be representative of physiological stimulation of post-ganglionic nerve stimulation.
|
['Animals', 'Autonomic Fibers, Postganglionic', 'Autonomic Fibers, Preganglionic', 'Autonomic Nervous System', 'Electric Stimulation', 'Enzyme Inhibitors', 'Female', 'Guinea Pigs', 'In Vitro Techniques', 'Male', 'Muscle, Smooth', 'Nitric Oxide Synthase', 'Nitric Oxide Synthase Type III', 'Nitroarginine', 'Synapses', 'Trachea', 'Vagus Nerve']
| 10,510,458
|
[['B01.050'], ['A08.675.542.100', 'A08.800.050.050.050', 'A08.800.800.060.050', 'A11.671.501.100'], ['A08.675.127.500.060', 'A08.675.542.234.060', 'A08.800.050.050.060', 'A08.800.800.060.060', 'A11.671.188.500.060', 'A11.671.501.234.060'], ['A08.800.050'], ['E05.723.402'], ['D27.505.519.389'], ['B01.050.150.900.649.313.992.550'], ['E05.481'], ['A02.633.570', 'A10.690.467'], ['D08.811.682.664.500.772'], ['D08.811.682.664.500.772.750'], ['D12.125.068.050.587', 'D12.125.095.104.587'], ['A08.850', 'A11.284.149.165.420.780'], ['A04.889'], ['A08.800.050.050.925', 'A08.800.050.600.825', 'A08.800.800.060.920', 'A08.800.800.120.900']]
|
['Organisms [B]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]']
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Human pituitary growth hormone (hGH) and Creutzfeldt-Jakob disease: results of an epidemiological survey in France, 1986.
|
An epidemiological inquiry has been done in France after the notification in the USA and England of four cases of Creutzfeldt-Jakob disease in patients previously treated with hGH. Between 1959, when hGH treatment in France was started, and August 1985, the date the survey began, 1698 patients were registered for treatment. Current information (less than three months old) was obtained for 1620 patients (95.4%). Death was reported in 31 patients, but none could be related to Creutzfeldt-Jakob or similar disease. Pathological events were observed in 213 living patients (13.1%). Among them, four were diseases classified as possibly related to a viral infection. The first case had acute lymphoid leukaemia; the second case had polyradiculoneuritis associated with hepatitis. In both cases the disease resolved completely. Two other patients had acute encephalitis which started less than two years after the onset of treatment and which resolved spontaneously. Even though the acute evolution and the spontaneous clinical recovery are not consistent with Creutzfeldt-Jakob disease, a relationship with hGH therapy could not be completely excluded. Finally, five treated children had later malignancies which raises the question of the long-term secondary effects of hGH upon cellular proliferation.
|
['Adolescent', 'Child', 'Child, Preschool', 'Creutzfeldt-Jakob Syndrome', 'Drug Contamination', 'Epidemiologic Methods', 'Female', 'France', 'Growth Hormone', 'Humans', 'Infant', 'Male', 'Virus Diseases']
| 3,042,652
|
[['M01.060.057'], ['M01.060.406'], ['M01.060.406.448'], ['C01.207.800.230', 'C10.228.140.380.165', 'C10.228.228.800.230', 'F03.615.400.300'], ['N06.850.360'], ['E05.318', 'N06.850.520'], ['Z01.542.286'], ['D06.472.699.631.525.425', 'D12.644.548.691.525.425'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703'], ['C01.925']]
|
['Named Groups [M]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Health Care [N]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Geographicals [Z]', 'Chemicals and Drugs [D]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Bilateral ocular scrofuloderma with orbital tuberculosis.
|
Ocular scrofuloderma with orbital tuberculosis is a rarely described presentation of childhood tuberculosis. Bilateral involvement has not been reported earlier in the medical literature. Here is reported a 3-year-old boy who presented with bilateral infraorbital swellings of tubercular etiology. Computed tomography (CT) scan of the upper face revealed enhancing soft tissue lesions in both the lower lids of the eyes, with extraconal extension into the orbits and with erosion of the right zygomatic bone. Tubercular etiology was confirmed by the Ziehl Neelsen staining of the aspirate from the lesion, which was positive for acid-fast bacilli and growth of Mycobacterium tuberculosis in the aspirate culture. The patient showed marked improvement of his lesions on anti-tubercular treatment.
|
['Child, Preschool', 'Humans', 'Male', 'Mycobacterium tuberculosis', 'Orbital Diseases', 'Tuberculosis, Cutaneous', 'Tuberculosis, Ocular']
| 16,816,501
|
[['M01.060.406.448'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['B03.510.024.962.500.702', 'B03.510.460.400.410.552.552.702'], ['C11.675'], ['C01.150.252.410.040.552.846.588', 'C01.150.252.819.820', 'C01.800.720.820', 'C17.800.838.765.820'], ['C01.150.252.289.800', 'C01.375.354.800', 'C11.294.354.800']]
|
['Named Groups [M]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
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Postsplinting x-rays of nondisplaced hand, wrist, ankle, and foot fractures are unnecessary.
|
BACKGROUND: Acute nondisplaced fractures (NDFs) are common in the emergency department (ED), and providers often obtain postsplinting x-rays to identify displacement that potentially occurs during the splinting process. Our objectives are to (1) determine how often x-rays are obtained after splinting of NDFs, (2) identify if postsplinting x-rays change treatment management in the ED, and (3) identify if there are medical complications at follow-up.METHODS: A retrospective chart review of ED patients who were discharged with hand, wrist, ankle, or foot fractures was conducted to determine patients with definite NDFs that were verified by a radiologist, underwent splinting, and either had postsplint x-rays or not. Bone displacement during the splinting procedure was determined by the postsplint x-rays in the ED. Internal movement of bones or management change was also determined for patients who did not undergo postsplint x-rays in the ED but had obtained an x-ray at their follow-up visit (in-network providers only).RESULTS: Our results demonstrate that no patients required further manipulation or operative management due to the splinting that occurred in the ED. These results take into account both patients who had postsplint x-rays conducted in the ED (27 patients) and those who received x-rays in follow-up consults (179 patients). There was minimal incidence of interval movement in the latter group (14 patients), none of which resulted in management change.CONCLUSION: These data conclude that postsplinting x-rays of NDFs are unnecessary. Removal of this procedure from routine practice will help decrease patient and hospital cost, time, and radiation exposure.
|
['Adolescent', 'Adult', 'Aged', 'Aged, 80 and over', 'Ankle Injuries', 'Child', 'Child, Preschool', 'Female', 'Foot Injuries', 'Fracture Fixation, Internal', 'Fractures, Bone', 'Hand Injuries', 'Humans', 'Infant', 'Male', 'Middle Aged', 'Postoperative Period', 'Radiography', 'Retrospective Studies', 'Splints', 'Wrist Injuries', 'Young Adult']
| 27,236,855
|
[['M01.060.057'], ['M01.060.116'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['C26.558.100'], ['M01.060.406'], ['M01.060.406.448'], ['C26.558.300'], ['E04.555.300.300'], ['C26.404'], ['C26.448'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703'], ['M01.060.116.630'], ['E04.614.750', 'N02.421.585.753.750'], ['E01.370.350.700'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E07.858.442.660.430.750', 'E07.858.690.725.430.750'], ['C26.088.906'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Health Care [N]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Experimental evaluation of anthracycline analogs.
|
This review summarizes the preclinical tests conducted to date for experimental evaluation of new anthracycline analogs. Most of the data are derived from the experience at the Istituto Nazionale Tumori, Milan, Italy, at the National Cancer Institute, Bethesda, Md, and at the Farmitalia Research Laboratories, Nerviano, Italy. In vitro cytotoxicity tests are useful for determining the doses to be used in vivo. Antitumor activity tests in mice can be divided into different stages. P388 and L1210 leukemias are generally used in primary screening; the value of adding L1210 leukemia is briefly discussed. Other experimental tumors adopted include disseminated leukemia and transplanted solid tumors. The importance of the route and schedule of treatment is stressed. Drugs should be administered iv in the case of solid tumors, and the schedule of treatment can be adjusted according to the pharmacokinetic properties of the new analog, when these are known. If possible, the parent compound and the new analog should be dissolved in the same solvents. In the toxicity tests, cardiac toxicity deserves particular attention. Until now, the only experimental model in which a number of new anthracyclines have been tested is the rat model proposed by Zbinden. A comparison between cardiotoxicity data obtained in such models and antitumor data obtained in mice shows that cardiac toxicity can be dissociated from the antitumor activity. Knowledge of pharmacokinetic properties of new analogs is of importance for selecting the schedules of treatment and for explaining selective toxic effects.
|
['Animals', 'Antibiotics, Antineoplastic', 'Daunorubicin', 'Disease Models, Animal', 'Doxorubicin', 'Drug Administration Schedule', 'Drug Evaluation, Preclinical', 'Glycosides', 'HeLa Cells', 'Heart', 'Leukemia L1210', 'Leukemia, Experimental', 'Mice', 'Naphthacenes', 'Neoplasms, Experimental', 'Sarcoma, Experimental']
| 455,325
|
[]
|
[]
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[Monoclonal Antibody Against N Terminal 439-451 Epitopes of Anti-Mullerian Hormone and Its Properties].
|
Objective: To prepare the specific monoclonal antibody against the N-terminal specific epitope peptide of anti-mullerian hormone (AMH) and to identify its specificity.Methods: Using bioinformatics analysis software to predict the specific peptide fragment of AMH. Then synthesized four antigenic epitope peptide segments of mature N-terminal region of AMH as the screening target antigen. Synthesized AMH wholegene.Using the prokaryotic expression system to abtain recombinant AMH protein. Immunized BALB/c mice with the recombinant AMH, and prepared mouse spleen cells for fusing with SP/20 cells. Preparation of AMH monoclonal antibody by hybridoma technology. The monoclonal antibodies against AMH were screened by using four N-terminal epitope peptides (1: 439-451 RGRDPRGPGRAQ, 2: 273-285 PPRPSAELEESPP, 3: 42-54 DLDWPPGSPQEPL, 4: 494-506 WPQSDRNPRYGNH) as antigens, and indirect ELISA and Western blot were used to identify the antigen binding characteristics of the selected monoclonal antibodies.Results: Two hybridoma cell lines with stable anti-AMH-1 and anti-AMH-2 antibody activities were screened. The two antibodies were named anti-AMH-1 and anti-AMH-2 respectively. The antibody titers were 1?12 000 and 1?1 600 after purification. Western blot confirmed that the two McAbs recognized different antigens. Anti-AMH-1 could not only recognize the N-terminal 439-451 epitope peptide of AMH, but also recognize the amino acid sequence of recombinant AMH, as well as the ovarian tissue. Anti-AMH-2 could recognize recombinant AMH and ovarian tissue.Conclusion: Two monoclonal antibodies against N-terminal specific epitopes of human AMH were successfully constructed.
|
['Animals', 'Anti-Mullerian Hormone', 'Antibodies, Monoclonal', 'Computational Biology', 'Epitopes', 'Humans', 'Hybridomas', 'Mice', 'Mice, Inbred BALB C']
| 32,691,556
|
[['B01.050'], ['D06.472.334.984.500', 'D09.400.430.625', 'D12.776.395.089'], ['D12.776.124.486.485.114.224', 'D12.776.124.790.651.114.224', 'D12.776.377.715.548.114.224'], ['H01.158.273.180', 'L01.313.124'], ['D23.050.550'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A11.251.353.485', 'A11.251.600.485'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.338', 'B01.050.150.900.649.313.992.635.505.500.400.338']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Disciplines and Occupations [H]', 'Information Science [L]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
|
Identification of a common 6-pyruvoyl-tetrahydropterin synthase mutation at codon 87 in Chinese phenylketonuria caused by tetrahydrobiopterin synthesis deficiency.
|
Deficiency in 6-pyruvoyl-tetrahydropterin synthase (PTPS) activity is the major cause of tetrahydrobiopterin (BH4)-deficient phenylketonuria. Two single base alterations of PTPS cDNA, a C-to-T transition at nucleotide 259 and a novel A-to-G transition at nucleotide 155 (according to cDNA sequence), were identified in two Chinese PTPS-deficient siblings by the reverse transcription-polymerase chain reaction (RT-PCR). The C-to-T transition at nucleotide 259 results in an amino acid change from proline to serine at codon 87 (Pro87Ser), and the A-to-G transition at nucleotide 155 causes an amino acid change from asparagine to serine at codon 52 (Asn52Ser) of PTPS. The C259T missense mutation can be identified by analysis of the BbvI restriction fragments of the PCR-amplified PTPS cDNA product, and was found to account for 42% (11/26) of 26 Chinese PTPS mutant alleles studied. However, none of 100 normal alleles screened were found to have this change. This result indicates that the C259T transition may be a common mutation in Chinese PTPS-deficient patients.
|
['Adult', 'Alcohol Oxidoreductases', 'Biopterin', 'Child', 'Child, Preschool', 'China', 'Codon', 'DNA, Complementary', 'Humans', 'Infant', 'Male', 'Mutation', 'Phenylketonurias', 'Phosphorus-Oxygen Lyases']
| 8,707,300
|
[['M01.060.116'], ['D08.811.682.047'], ['D03.633.100.733.631.202', 'D08.211.090'], ['M01.060.406'], ['M01.060.406.448'], ['Z01.252.474.164'], ['D13.444.735.544.355', 'G05.360.335.355', 'G05.360.340.024.340.137.190'], ['D13.444.308.497.220', 'D13.444.600.223.500', 'D27.720.470.530.600.223.260'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703'], ['G05.365.590'], ['C10.228.140.163.100.687', 'C16.320.565.100.766', 'C16.320.565.189.687', 'C18.452.132.100.687', 'C18.452.648.100.766', 'C18.452.648.189.687'], ['D08.811.520.650']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Geographicals [Z]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 1
|
Stimulation of bronchoalveolar lavage (BAL) and blood lymphocytes by Kveim antigen, tuberculin and concanavalin A in sarcoidosis.
|
BAL and blood mononuclear cells and their reactivity to Kveim antigen, tuberculin and concanavalin A (Con A) were studied in nine patients with different clinical stages of sarcoidosis. After separation by plastic adherence, non-adherent cells (mainly lymphocytes) were admixed with 10% autologous adherent cells (monocytes/macrophages). After 3 and 6 days' culture with Kveim antigen (1, 10, 100 micrograms/ml), PPD tuberculin (2.5 micrograms/ml) and Con A (10, 20, 40 micrograms/ml) stimulation was measured as incorporation of 14C-thymidine into DNA. Except for occasional reactions the study did not show any unitary significant increase in lymphocyte response to the different concentrations of Kveim antigen in either BAL or blood. For Con A there was a weaker response by BAL-mononuclear cells with no difference between 3 and 6 days, compared with blood where there was an early peak. The lymphocyte reaction to PPD was weak with no difference between blood and BAL.
|
['Adult', 'Bronchoalveolar Lavage Fluid', 'Concanavalin A', 'Female', 'Humans', 'Kveim Test', 'Leukocyte Count', 'Lung Diseases', 'Lymphocyte Activation', 'Lymphocytes', 'Male', 'Middle Aged', 'Sarcoidosis', 'Skin Tests', 'Stimulation, Chemical', 'Tuberculin']
| 3,256,351
|
[['M01.060.116'], ['E05.927.100.500'], ['D12.776.503.499.500', 'D12.776.765.678.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.225.812.871.300.540', 'E05.200.812.871.300.540', 'E05.478.594.890.300.540'], ['E01.370.225.500.195.107.595', 'E01.370.225.625.107.595', 'E05.200.500.195.107.595', 'E05.200.625.107.595', 'E05.242.195.107.595', 'G04.140.107.595', 'G09.188.105.595'], ['C08.381'], ['E01.370.225.812.482', 'E05.200.812.482', 'E05.478.594.530', 'G12.450.050.400.545', 'G12.565'], ['A11.118.637.555.567', 'A15.145.229.637.555.567', 'A15.382.490.555.567'], ['M01.060.116.630'], ['C15.604.515.827'], ['E01.370.225.812.871', 'E05.200.812.871', 'E05.478.594.890'], ['G07.690.773.996'], ['D23.050.161.845']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Annexin-A1 enhances breast cancer growth and migration by promoting alternative macrophage polarization in the tumour microenvironment.
|
Macrophages are potent immune cells with well-established roles in the response to stress, injury, infection and inflammation. The classically activated macrophages (M1) are induced by lipopolysaccharide (LPS) and express a wide range of pro-inflammatory genes. M2 macrophages are induced by T helper type 2 cytokines such as interleukin-4 (IL4) and express high levels of anti-inflammatory and tissue repair genes. The strong association between macrophages and tumour cells as well as the high incidences of leukocyte infiltration in solid tumours have contributed to the discovery that tumour-associated macrophages (TAMs) are key to tumour progression. Here, we investigated the role of Annexin A1 (ANXA1), a well characterized immunomodulatory protein on macrophage polarization and the interaction between macrophages and breast cancer cells. Our results demonstrate that ANXA1 regulates macrophage polarization and activation. ANXA1 can act dually as an endogenous signalling molecule or as a secreted mediator which acts via its receptor, FPR2, to promote macrophage polarization. Furthermore, ANXA1 deficient mice exhibit reduced tumour growth and enhanced survival in vivo, possibly due to increased M1 macrophages within the tumor microenvironment. These results provide new insights into the molecular mechanisms of macrophage polarization with therapeutic potential to suppress breast cancer growth and metastasis.
|
['Animals', 'Annexin A1', 'Cell Movement', 'Cell Proliferation', 'Extracellular Signal-Regulated MAP Kinases', 'Female', 'Macrophages', 'Mammary Neoplasms, Animal', 'Mice', 'NF-kappa B', 'Receptors, Formyl Peptide', 'Signal Transduction', 'Tumor Cells, Cultured', 'Tumor Microenvironment']
| 29,263,330
|
[['B01.050'], ['D12.776.157.125.050.050'], ['G04.198', 'G07.568.500.180'], ['G04.161.750', 'G07.345.249.410.750'], ['D08.811.913.696.620.682.700.567.249', 'D12.644.360.450.169', 'D12.776.476.450.169'], ['A11.329.372', 'A11.627.482', 'A11.733.397', 'A15.382.670.522', 'A15.382.680.397'], ['C04.588.531', 'C22.520'], ['B01.050.150.900.649.313.992.635.505.500'], ['D05.500.672', 'D12.776.260.600', 'D12.776.660.600', 'D12.776.930.600'], ['D12.776.543.750.695.235', 'D12.776.543.750.705.873', 'D12.776.543.750.750.340'], ['G02.111.820', 'G04.835'], ['A11.251.860'], ['G04.366.500']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
In Silico Prediction of Cytochrome P450-Drug Interaction: QSARs for CYP3A4 and CYP2C9.
|
Cytochromes P450 (CYP) are the main actors in the oxidation of xenobiotics and play a crucial role in drug safety, persistence, bioactivation, and drug-drug/food-drug interaction. This work aims to develop Quantitative Structure-Activity Relationship (QSAR) models to predict the drug interaction with two of the most important CYP isoforms, namely 2C9 and 3A4. The presented models are calibrated on 9122 drug-like compounds, using three different modelling approaches and two types of molecular description (classical molecular descriptors and binary fingerprints). For each isoform, three classification models are presented, based on a different approach and with different advantages: (1) a very simple and interpretable classification tree; (2) a local (k-Nearest Neighbor) model based classical descriptors and; (3) a model based on a recently proposed local classifier (N-Nearest Neighbor) on binary fingerprints. The salient features of the work are (1) the thorough model validation and the applicability domain assessment; (2) the descriptor interpretation, which highlighted the crucial aspects of P450-drug interaction; and (3) the consensus aggregation of models, which largely increased the prediction accuracy.
|
['Animals', 'Computer Simulation', 'Cytochrome P-450 CYP2C9', 'Cytochrome P-450 CYP2C9 Inhibitors', 'Cytochrome P-450 CYP3A', 'Cytochrome P-450 CYP3A Inhibitors', 'Humans', 'Protein Binding', 'Quantitative Structure-Activity Relationship']
| 27,294,921
|
[['B01.050'], ['L01.224.160'], ['D08.244.453.491.500.500', 'D08.811.682.690.708.170.450.500.500', 'D12.776.422.220.453.491.500.500'], ['D27.505.389.500.315', 'D27.505.519.389.335.329'], ['D08.244.453.860.500', 'D08.811.682.662.582.353', 'D08.811.682.690.708.170.495.500', 'D12.776.422.220.453.860.500'], ['D27.505.389.500.503', 'D27.505.519.389.335.503'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G02.111.679', 'G03.808'], ['G02.111.830.500', 'G07.690.773.997.500']]
|
['Organisms [B]', 'Information Science [L]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Routing Physarum with repellents.
|
Plasmodium of Physarum polycephalum is a single cell with many nuclei. Plasmodium is an easy-to-experiment-with biological substrate, a multi-functional bio-material used to implement novel and future computing architectures. The plasmodium exhibits typical features of excitable chemical systems and capable for distributed sensing, parallel information processing and decentralized actuation. Plasmodium of P. polycephalum is proved to be a universal storage modification machine. Actively growing zones of the plasmodium are considered to be elementary processors of the growing computing machine, as well as messages traveling in the spatially extended non-linear medium. Controlling propagation of the messages and computing processes is a prerequisite for a successful implementation of working prototypes of plasmodium machines. In laboratory experiments and computer simulation we show that active growing zones of plasmodium can be precisely routed using repelling diffusion gradients generated by crystals of sodium chloride. We demonstrate how to achieve controllable reflection, splitting/multiplication and merging of plasmodium's active zones.
|
['Antiprotozoal Agents', 'Computers, Molecular', 'Diffusion', 'Physarum polycephalum', 'Sodium Chloride']
| 20,401,510
|
[['D27.505.954.122.250.100'], ['L01.224.230.260.315', 'L01.224.300'], ['G01.202', 'G02.196'], ['B01.046.550.550.600.700.550'], ['D01.210.450.150.875', 'D01.857.650']]
|
['Chemicals and Drugs [D]', 'Information Science [L]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Dietary amino acid profiles and growth performance in juvenile kuruma prawn Marsupenaeus japonicus.
|
To assess the reference dietary amino acid profiles for juvenile kuruma prawn Marsupenaeus japonicus a feeding trial was conducted using six semi-purified diets containing casein-gelatin and pre-coated supplemental crystalline amino acids (CAA) and a control diet containing intact protein (casein-gelatin). Pre-coated CAA were supplemented to the diets to simulate dietary amino acid profiles to those of the prawn egg protein (PEP), prawn larvae whole body protein (PLP), prawn juvenile whole body protein (PJP), squid meal protein (SMP), short-necked clam protein (SNP) and brown fish meal protein (BFP). The result showed that kuruma prawn juveniles are capable of utilizing the pre-coated CAA and higher growth performances were observed in the groups fed the PJP, SMP and the control diets than those fed the PLP, SNP, BFP and PEP diets. The essential amino acid proportions (A/E ratios) of the whole body of kuruma prawn differ slightly when compared with the other penaeids or freshwater prawn. The results suggest that PJP and SMP would be suitable as a reference dietary amino acid profile for juvenile prawn.
|
['Amino Acids', 'Animal Feed', 'Animals', 'Body Composition', 'Body Weight', 'Decapoda', 'Dietary Proteins', 'Growth', 'Nutritional Requirements']
| 12,431,396
|
[['D12.125'], ['G07.203.300.300.100', 'J02.500.300.100'], ['B01.050'], ['G02.111.130', 'G03.180', 'G07.100.049'], ['C23.888.144', 'E01.370.600.115.100.160.120', 'E05.041.124.160.750', 'G07.100.100.160.120', 'G07.345.249.314.120'], ['B01.050.500.131.365.190'], ['D12.776.256', 'G07.203.300.428', 'J02.500.428'], ['G07.345.249'], ['G07.203.650.620']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Technology, Industry, and Agriculture [J]', 'Organisms [B]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Treatment and justice.
|
Support for the belief that criminal behaviour was amenable to treatment served different purposes: for developing psychiatry it offered a module of power to be exploited whereas for the State it was a convenient disguise for the infliction of pain which was the real intention of punishment. Having served its purpose the treatment approach is being abandoned in favour of a philosophy of just deserts which is more acceptable in the present political climate.
|
['Crime', 'Criminal Law', 'Humans', 'Jurisprudence', 'Mental Disorders', 'Patient Compliance', 'Punishment', 'Social Adjustment', 'United Kingdom']
| 4,016,399
|
[['I01.198.240', 'I01.880.735.191'], ['I01.198.290', 'I01.880.604.583.100'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['I01.880.604.583', 'N03.706.535'], ['F03'], ['F01.100.150.750.500.600', 'F01.145.488.887.500.600', 'N05.300.150.800.500.600'], ['F02.463.425.770.571', 'I01.880.630.716'], ['F01.145.813.621'], ['Z01.542.363']]
|
['Anthropology, Education, Sociology, and Social Phenomena [I]', 'Organisms [B]', 'Health Care [N]', 'Psychiatry and Psychology [F]', 'Geographicals [Z]']
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 1
|
Chlamydia pneumoniae multiply in neutrophil granulocytes and delay their spontaneous apoptosis.
|
The obligate intracellular bacterial pathogen Chlamydia pneumoniae (Cp) is responsible for a range of human diseases, including acute respiratory infection. Although experimental intratracheal infection with Cp results in a massive recruitment of neutrophil granulocytes (polymorphonuclear neutrophils (PMN)), the role of these cells in the defense against Cp is unclear. In this study the interactions of PMN with Cp were investigated. In vitro coincubation experiments showed that human granulocytes were able to internalize Chlamydia in an opsonin-independent manner. Importantly, phagocytosed Cp were not killed; the ingested bacteria survived and multiplied within PMN. Although uninfected granulocytes became apoptotic within 10 h, infected PMN survived up to 90 h. Coincubation with Cp significantly decreased the ratio of apoptotic PMN, as detected by morphological analysis, annexin V, and TUNEL staining. The observed antiapoptotic effect was associated with a markedly lower level of procaspase-3 processing and, consequently, reduced caspase-3 activity in infected PMN. LPS was found as a major, but not exclusive, component responsible for the observed antiapoptotic effect. Chlamydia LPS affected PMN apoptosis both by acting directly on the cells and by inducing the autocrine production of the antiapoptotic cytokine IL-8. These data show that, in contrast to other microbial pathogens that drive phagocytes into apoptosis to escape killing, Cp can extend the life span of neutrophil granulocytes, making them suitable host cells for survival and multiplication within the first hours/days after infection.
|
['Adult', 'Apoptosis', 'Caspase 3', 'Caspase Inhibitors', 'Caspases', 'Cell Communication', 'Cell Survival', 'Cell-Free System', 'Cells, Cultured', 'Chlamydophila pneumoniae', 'Coculture Techniques', 'Down-Regulation', 'Enzyme Precursors', 'Hot Temperature', 'Humans', 'Interleukin-8', 'Intracellular Fluid', 'Lipopolysaccharides', 'Neutrophils', 'Phagocytosis', 'Protein Processing, Post-Translational', 'Recombinant Proteins', 'Time Factors']
| 14,734,760
|
[['M01.060.116'], ['G04.146.954.035'], ['D08.811.277.656.262.500.126.350.300', 'D08.811.277.656.300.200.126.350.300', 'D12.644.360.075.405.350.300', 'D12.776.476.075.405.350.300'], ['D27.505.519.389.745.325.500'], ['D08.811.277.656.262.500.126', 'D08.811.277.656.300.200.126', 'D12.644.360.075.405', 'D12.776.476.075.405'], ['G04.085'], ['G04.346'], ['A11.284.835.168'], ['A11.251'], ['B03.440.190.190.230.249'], ['E05.481.500.374'], ['G02.111.240', 'G05.308.200', 'G07.690.773.937'], ['D08.622', 'D12.776.811.243'], ['G01.906.595.543', 'G16.500.275.063.725.710.380', 'G16.500.750.775.710.380', 'N06.230.300.100.725.232', 'N06.230.300.100.725.710.380'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.644.276.374.200.120.800', 'D12.644.276.374.465.312', 'D12.776.467.374.200.120.800', 'D12.776.467.374.465.246', 'D23.125.300.120.800', 'D23.469.200.120.800', 'D23.529.374.200.120.800', 'D23.529.374.465.312'], ['A11.284.430.429', 'A11.284.835.450', 'A12.207.515'], ['D09.400.500', 'D09.698.718.450', 'D10.494', 'D23.050.161.616.525', 'D23.946.123.329.500'], ['A11.118.637.415.583', 'A11.627.340.583', 'A11.733.689', 'A15.145.229.637.415.583', 'A15.382.490.315.583', 'A15.382.680.689'], ['G04.417.350', 'G09.188.665', 'G12.450.564.809', 'G12.688'], ['G02.111.660.871.790.600', 'G02.111.691.600', 'G03.734.871.790.600', 'G05.308.670.600'], ['D12.776.828'], ['G01.910.857']]
|
['Named Groups [M]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Microstructure of Pharmaceutical Semicrystalline Dispersions: The Significance of Polymer Conformation.
|
The microstructure of pharmaceutical semicrystalline solid dispersions has attracted extensive attention due to its complexity that might result in the diversity in physical stability, dissolution behavior, and pharmaceutical performance of the systems. Numerous factors have been reported that dictate the microstructure of semicrystalline dispersions. Nevertheless, the importance of the complicated conformation of the polymer has never been elucidated. In this study, we investigate the microstructure of dispersions of polyethylene glycol and active pharmaceutical ingredients by small-angle X-ray scattering and high performance differential scanning calorimetry. Polyethylene glycol with molecular weight of 2000 g/mol (PEG2000) and 6000 g/mol (PEG6000) exhibited remarkable discrepancy in the lamellar periodicity in dispersions with APIs which was attributed to the differences in their folding behavior. The long period of PEG2000 always decreased upon aging-induced exclusion of APIs from the interlamellar region of extended chain crystals whereas the periodicity of PEG6000 may decrease or increase during storage as a consequence of the competition between the drug segregation and the lamellar thickening from nonintegral-folded into integral-folded chain crystals. These processes were in turn significantly influenced by the crystallization tendency of the pharmaceutical compounds, drug-polymer interactions, as well as the dispersion composition and crystallization temperature. This study highlights the significance of the polymer conformation on the microstructure of semicrystalline systems that is critical for the preparation of solid dispersions with consistent and reproducible quality.
|
['Calorimetry, Differential Scanning', 'Chemistry, Pharmaceutical', 'Drug Liberation', 'Drug Stability', 'Molecular Conformation', 'Molecular Weight', 'Polymers', 'X-Ray Diffraction']
| 29,320,195
|
[['E05.196.131.310', 'E05.196.370.310'], ['H01.158.703.007', 'H01.181.466'], ['G02.211', 'G03.787.321', 'G07.690.725.321'], ['E05.916.330'], ['G02.111.570.820'], ['G02.494'], ['D05.750', 'D25.720', 'J01.637.051.720'], ['E05.196.309.742', 'E05.196.822.950', 'G01.867.950', 'G02.965']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Technology, Industry, and Agriculture [J]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
|
[Changes in porphyrin metabolism of mice given beryllium and/or zinc].
|
Beryllium chloride and/or zinc chloride were intraperitoneally injected into mice. The amount of beryllium (Be) injected corresponded to 1/10th of the LD50 dose intravenously administered. The amount of zinc (Zn) injected was the same as Be. The changes in porphyrin metabolism of the mice were studied. Delta-aminolevulinic acid dehydratase (ALA-D) activities in the blood were found to increase significantly in Zn and BeZn groups when compared to the control level. The blood porphobilinogen deaminase (PBG-D) activity in the Zn group was slightly less than that in the controls. The ALA-D and PBG-D activities in liver were higher in the Be and BeZn groups than in the controls. The splenic ALA-D activities were significantly higher in the Zn and BeZn groups than in the control and Be groups. The splenic PBG-D activities were markedly higher in the Be and/or Zn groups than in the controls. An increase in ALA-D activities in the blood and spleen was observed in the BeZn group, together with an increase in ALA-D activities caused by Zn administration. Furthermore, the increase in PBG-D activities in liver and spleen was observed in the Be and/or Zn groups. The results suggested that chemical similarity between Be and Zn brought about these phenomena.
|
['Animals', 'Beryllium', 'Chlorides', 'Hydroxymethylbilane Synthase', 'Liver', 'Male', 'Mice', 'Mice, Inbred ICR', 'Porphobilinogen Synthase', 'Porphyrins', 'Spleen', 'Zinc Compounds']
| 9,211,591
|
[['B01.050'], ['D01.268.552.075', 'D01.268.557.080', 'D01.552.547.080'], ['D01.210.450.150', 'D01.248.497.158.215'], ['D08.811.913.225.575'], ['A03.620'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.510', 'B01.050.150.900.649.313.992.635.505.500.400.510'], ['D08.811.520.241.300.550'], ['D03.383.129.578.840.500', 'D03.633.400.909.500', 'D04.345.783.500', 'D23.767.727'], ['A10.549.700', 'A15.382.520.604.700'], ['D01.975']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Change and Innovation in the Funeral Industry.
|
The "modern" or traditional funeral, as it is known in the funeral industry today, that includes embalming, casket, service, and burial in a cemetery, emerged as a result of four forces in American society: the Industrial Revolution, the Civil War, the emergence of a genteel code of conduct as a result of increased wealth in our society, and changing cultural views toward death. While the traditional funeral ritual remains the most popular funeral selection in the United States today, the industry is experiencing changes that are reshaping the death rituals and methods of body disposal. A meta-analysis of relevant news articles from 1987 through 2014 finds that these changes are occurring as a result of two general motivational themes: a Business-Related Motivation and a Consumer-Related Motivation, each with corresponding subthemes.
|
['Attitude to Death', 'Funeral Rites', 'History, 17th Century', 'History, 18th Century', 'History, 19th Century', 'History, 20th Century', 'History, 21st Century', 'Humans', 'Organizational Innovation', 'United States']
| 28,395,641
|
[['F01.100.125', 'N05.300.125'], ['I01.076.201.450.550'], ['K01.400.504.750'], ['K01.400.504.875'], ['K01.400.504.937'], ['K01.400.504.968'], ['K01.400.504.984'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['N04.452.610'], ['Z01.107.567.875']]
|
['Psychiatry and Psychology [F]', 'Health Care [N]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Humanities [K]', 'Organisms [B]', 'Geographicals [Z]']
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 1
|
What predicts depression in cardiac patients: sociodemographic factors, disease severity or theoretical vulnerabilities?
|
Depression is associated with increased cardiovascular risk in acute coronary syndrome (ACS) patients, but some argue that elevated depression is actually a marker of cardiovascular disease severity. Therefore, disease indices should better predict depression than established theoretical causes of depression (interpersonal life events, reinforcing events, cognitive distortions, type D personality). However, little theory-based research has been conducted in this area. In a cross-sectional design, ACS patients (n = 336) completed questionnaires assessing depression and psychosocial vulnerabilities. Nested logistic regression assessed the relative contribution of demographic or vulnerability factors, or disease indices or vulnerabilities to depression. In multivariate analysis, all vulnerabilities were independent significant predictors of depression (scoring above threshold on any scale, 48%). Demographic variables accounted for <1% of the variance of depression status, with vulnerabilities accounting for significantly more (pseudo R² = 0.16, ÷²(change) = 150.9, df = 4, p < 0.001). Disease indices accounted for 7% of the variance in depression (pseudo R² = 0.07, ÷² = 137.9, p < 0.001). However, adding the vulnerabilities increased the overall variance explained to 22% (pseudo R² = 0.22, ÷² = 58.6, df = 4, p < 0.001). Theoretical vulnerabilities predicted depression status better than did either demographic or disease indices. The presence of these proximal causes of depression suggests that depression in ACS patients is not simply a result of cardiovascular disease severity.
|
['Acute Coronary Syndrome', 'Adaptation, Psychological', 'Aged', 'Angina, Unstable', 'Cross-Sectional Studies', 'Culture', 'Depressive Disorder', 'Disability Evaluation', 'Female', 'Humans', 'Internal-External Control', 'Life Change Events', 'Male', 'Middle Aged', 'Myocardial Infarction', 'Personality Inventory', 'Psychometrics', 'Risk Factors', 'Sick Role', 'Socioeconomic Factors', 'Temperament']
| 21,038,172
|
[['C14.280.647.124', 'C14.907.585.124'], ['F01.058'], ['M01.060.116.100'], ['C14.280.647.187.150', 'C14.907.585.187.150', 'C23.888.592.612.233.500.150'], ['E05.318.372.500.875', 'N05.715.360.330.500.875', 'N06.850.520.450.500.875'], ['I01.076.201.450', 'I01.880.853.100'], ['F03.600.300'], ['E01.370.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F01.829.379'], ['F01.829.458.410'], ['M01.060.116.630'], ['C14.280.647.500', 'C14.907.585.500', 'C23.550.513.355.750', 'C23.550.717.489.750'], ['F04.711.647.513'], ['F04.711.780'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['F01.829.316.616.751'], ['I01.880.853.996', 'N01.824'], ['F01.752.898']]
|
['Diseases [C]', 'Psychiatry and Psychology [F]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 0
|
Sex-sorted bovine spermatozoa and DNA damage: I. Static features.
|
This study examined the static response of Spermatozoa DNA Fragmentation (SDF) after sex selection in bulls using a MoFlo(®) SX (Beckman Coulter, Miami FL) spermatozoa sorter to produce three different subpopulations: 1) Spermatozoa bearing X- chromosomes with a purity of 95%, 2) Spermatozoa bearing Y-chromosomes with a purity of 95%, and 3) non-viable spermatozoa. The static response of SDF refers to the baseline values observed for DNA damage when analyzed pre- and post sex-sorting. Results showed that while the baseline level SDF in pre-sorted bull spermatozoa samples ranged from 5.3% to 11% with an average of 7.9% ± 2.1%, the level of SDF obtained in X- and Y-chromosome sorted samples was much lower (3.1% ± 1.9%) and statistical differences were obtained after comparing both groups (P < 0.01). Spermatozoa containing a fragmented DNA molecule tend to be accumulated in the non-viable subpopulation. The baseline SDF level in X- and Y-chromosome sorted subpopulations is reduced, by 63% on average when compared to the values obtained in the neat semen sample. Different bulls exhibit unique SDF reduction efficiencies via the X- and Y-chromosome sex selection process.
|
['Animals', 'Cattle', 'Cell Separation', 'DNA Damage', 'DNA Fragmentation', 'Flow Cytometry', 'Male', 'Quality Control', 'Semen Analysis', 'Sex Preselection', 'Spermatozoa', 'Static Electricity']
| 20,932,559
|
[['B01.050'], ['B01.050.150.900.649.313.500.380.271'], ['E01.370.225.500.363', 'E05.200.500.363', 'E05.242.363'], ['G05.200'], ['G05.200.230'], ['E01.370.225.500.363.342', 'E01.370.225.500.386.350', 'E05.196.712.516.600.240.350', 'E05.200.500.363.342', 'E05.200.500.386.350', 'E05.242.363.342', 'E05.242.386.350'], ['J01.897.608'], ['E01.370.225.992', 'E05.200.992'], ['E05.393.420.890'], ['A05.360.490.890', 'A11.497.760'], ['G01.358.500.249.820']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Technology, Industry, and Agriculture [J]', 'Anatomy [A]']
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Indirect adaptive soft computing based wavelet-embedded control paradigms for WT/PV/SOFC in a grid/charging station connected hybrid power system.
|
This paper focuses on the indirect adaptive tracking control of renewable energy sources in a grid-connected hybrid power system. The renewable energy systems have low efficiency and intermittent nature due to unpredictable meteorological conditions. The domestic load and the conventional charging stations behave in an uncertain manner. To operate the renewable energy sources efficiently for harvesting maximum power, instantaneous nonlinear dynamics should be captured online. A Chebyshev-wavelet embedded NeuroFuzzy indirect adaptive MPPT (maximum power point tracking) control paradigm is proposed for variable speed wind turbine-permanent synchronous generator (VSWT-PMSG). A Hermite-wavelet incorporated NeuroFuzzy indirect adaptive MPPT control strategy for photovoltaic (PV) system to extract maximum power and indirect adaptive tracking control scheme for Solid Oxide Fuel Cell (SOFC) is developed. A comprehensive simulation test-bed for a grid-connected hybrid power system is developed in Matlab/Simulink. The robustness of the suggested indirect adaptive control paradigms are evaluated through simulation results in a grid-connected hybrid power system test-bed by comparison with conventional and intelligent control techniques. The simulation results validate the effectiveness of the proposed control paradigms.
|
['Algorithms', 'Electric Power Supplies', 'Electricity', 'Models, Theoretical', 'Oxides', 'Wind']
| 28,877,191
|
[['G17.035', 'L01.224.050'], ['E07.305.124'], ['G01.358.500.249'], ['E05.599'], ['D01.248.497.158.685', 'D01.650.550'], ['G16.500.175.249.200', 'G16.500.275.063.725.154.200', 'G16.500.750.775.780', 'N06.230.132.644.875', 'N06.230.300.100.150.185.200', 'N06.230.300.100.725.780']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Health Care [N]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 1
| 0
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Mortality and complications in very old patients (90+) admitted to departments of internal medicine in Spain.
|
SUMMARY: Patients over 90 years of age (the "very elderly") account for an increasing number of admissions to departments of internal medicine (IM). The aim of this study was to analyse the demographic data, hospitalization characteristics, medical complications, and predictors of mortality in patients over 90 admitted to IM departments.MATERIAL AND METHODS: All patients admitted to IM departments in Spain between the years 2005 and 2007 were analysed. Clinical and demographic data were compared with records from "younger elderly" patients (65-90).RESULTS: During the study period, there were 1,567,659 patient admissions to IM departments in Spain, and 90,679 (5.8%) were older than 90. Hospital mortality occurred in 22.3% of very elderly patients. The main predictors for hospital death were pressure ulcer (Odds Ratio [OR] 1.55, CI95% 1.45-1.66), thromboembolic disease (OR 1.83, CI95% 1.61-2.09), nosocomial pneumonia (OR 2.53, CI95% 2.39-2.69), hip fracture (OR 2.20, CI95% 1.53-3.18), male gender (OR 1.06, CI95% 1.03-1.10), age (OR 1.05, CI95% 1.04-1.06), dementia (OR 1.13, CI95% 1.08-1.18), cancer (OR 1.60, CI95% 1.51-1.71), acute respiratory failure (OR 1.83, CI95% 1.76-1.89), acute infectious disease (OR 2.30, IC95% 2.11-2.52), and Charlson comorbidity index (OR 1.21, CI95% 1.16-1.26).CONCLUSIONS: Very elderly patients represent a large and growing fraction of the total admissions to IM departments in Spain. They are at higher risk for complications during their hospital stay and mortality rate is double that of the younger elderly.
|
['Age Distribution', 'Aged, 80 and over', 'Cohort Studies', 'Communicable Diseases', 'Cross Infection', 'Dementia', 'Female', 'Hip Fractures', 'Hospital Departments', 'Hospital Mortality', 'Humans', 'Inpatients', 'Internal Medicine', 'Male', 'Neoplasms', 'Odds Ratio', 'Patient Admission', 'Patient Discharge', 'Pressure Ulcer', 'Respiratory Insufficiency', 'Retrospective Studies', 'Risk Factors', 'Sex Distribution', 'Spain', 'Thromboembolism']
| 21,238,893
|
[['I01.240.050', 'N01.224.033', 'N06.850.505.400.050'], ['M01.060.116.100.080'], ['E05.318.372.500.750', 'N05.715.360.330.500.750', 'N06.850.520.450.500.750'], ['C01.221', 'C23.550.291.531'], ['C01.248', 'C23.550.291.875.500'], ['C10.228.140.380', 'F03.615.400'], ['C26.404.061.425', 'C26.531.750', 'C26.558.276.425'], ['N02.278.216.500.968', 'N04.452.442.452.422'], ['E05.318.308.985.550.400', 'N01.224.935.698.400', 'N06.850.505.400.975.550.400', 'N06.850.520.308.985.550.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.643.470'], ['H02.403.429'], ['C04'], ['E05.318.740.600.600', 'G17.680.500', 'N05.715.360.750.625.590', 'N06.850.520.830.600.600'], ['E02.760.400.600', 'N02.421.585.400.600'], ['E02.760.169.125', 'E02.760.400.610', 'N02.421.585.169.125', 'N02.421.585.400.610', 'N04.590.233.727.210.125'], ['C17.800.893.665'], ['C08.618.846'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['I01.240.800', 'N01.224.803', 'N06.850.505.400.850'], ['Z01.542.846'], ['C14.907.355.590']]
|
['Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Organisms [B]', 'Disciplines and Occupations [H]', 'Phenomena and Processes [G]', 'Geographicals [Z]']
| 0
| 1
| 1
| 0
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 1
| 1
|
HLA-DP4, the most frequent HLA II molecule, defines a new supertype of peptide-binding specificity.
|
Among HLA-DP specificities, HLA-DP4 specificity involves at least two molecules, HLA-DPA1*0103/DPB1*0401 (DP401) and HLA-DPA1*0103/DPB1*0402 (DP402), which differ from each other by only three residues. Together, they are present worldwide at an allelic frequency of 20-60% and are the most abundant human HLA II alleles. Strikingly, the peptide-binding specificities of these molecules have never been investigated. Hence, in this study, we report the peptide-binding motifs of both molecules. We first set up a binding assay specific for the immunopurified HLA-DP4 molecules. Using multiple sets of synthetic peptides, we successfully defined the amino acid preferences of the anchor residues. With these assays, we were also able to identify new peptide ligands from allergens and viral and tumor Ags. DP401 and DP402 exhibit very similar patterns of recognition in agreement with molecular modeling of the complexes. Pockets P1 and P6 accommodate the main anchor residues and interestingly contain only two polymorphic residues, beta86 and beta11, respectively. Both positions are almost dimorphic and thus produce a limited number of pocket combinations. Taken together, our results support the existence of three main binding supertypes among HLA-DP molecules and should significantly contribute to the identification of universal epitopes to be used in peptide-based vaccines for cancer, as well as for allergic or infectious diseases.
|
['Amino Acid Motifs', 'Amino Acid Sequence', 'Amino Acid Substitution', 'Cell Line', 'Epitopes', 'Gene Frequency', 'HLA-DP Antigens', 'HLA-DP beta-Chains', 'Histocompatibility Testing', 'Humans', 'Models, Molecular', 'Molecular Sequence Data', 'Mutation', 'Peptides', 'Polymorphism, Genetic', 'Protein Binding', 'Sequence Alignment']
| 12,471,126
|
[['G02.111.570.820.709.275.500', 'G02.111.570.820.709.600.500'], ['G02.111.570.060', 'L01.453.245.667.060'], ['E05.393.420.601.035', 'G05.558.109'], ['A11.251.210'], ['D23.050.550'], ['G05.330'], ['D12.776.395.550.509.400.420', 'D12.776.543.550.440.400.420', 'D23.050.301.500.400.400.420', 'D23.050.301.500.450.400.420', 'D23.050.705.552.410.400.420', 'D23.050.705.552.450.400.420'], ['D12.776.395.550.509.400.420.750', 'D12.776.543.550.440.400.420.750', 'D23.050.301.500.400.400.420.750', 'D23.050.301.500.450.400.420.750', 'D23.050.705.552.410.400.420.750', 'D23.050.705.552.450.400.420.750'], ['E01.370.225.812.385', 'E05.200.812.385', 'E05.478.594.385'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.599.595'], ['L01.453.245.667'], ['G05.365.590'], ['D12.644'], ['G05.365.795'], ['G02.111.679', 'G03.808'], ['E05.393.751']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Organisms [B]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Preterm delivery risk in migrants in Italy: an observational prospective study.
|
BACKGROUND: Various studies have ascertained different birth outcomes between resident and migrant populations in western countries. Considering preterm delivery (<37 complete weeks of gestation) as a perinatal risk condition, we assessed its rate in migrant and native Italian women who delivered in the main public hospital in Brescia (Italy).METHODS: All migrant puerperas and a random sample of native puerperas hospitalized during the period February to May 2005 were included in the study after informed consent and filled in a self-administered multilanguage questionnaire enquiring about sociodemographic and obstetric data. Additional information including last menstrual period was obtained from personal obstetric records.RESULTS: As many as 471 puerperas entered the study: 366 Italian and 105 migrant women coming from eastern Europe (41.9%), Asia (20%), South America (10.5%), and Africa (27.6%). Of the migrant population, 67 of 105 (63.8%) were at their first delivery in Italy (median interval from arrival: 3.8 y). Gestational age at delivery was assessed for 456 of 471 women (103 migrants and 353 Italians). A total of 36 (7.9%) preterm deliveries were registered: 22 (6.2%) in Italian and 14 (13.6%) in migrant puerperas (p value = 0.015). The highest preterm delivery rate was observed in African women (20.7%), while women from eastern Europe had a similar rate to Italians. In univariate analysis, factors associated to preterm delivery were parity and length of permanence in Italy. We could not demonstrate any correlation with smoking or with a delayed access to antenatal care (first obstetric evaluation after 12 complete weeks of gestation). In multivariate analysis, African origin was the only independent risk factor for preterm delivery [odds ratio (OR) = 3.54; p = 0.018].CONCLUSIONS: In our setting, preterm delivery occurred more frequently in migrant women, particularly of African origin, and it is not associated to delayed access to antenatal care.
|
['Adult', 'Africa', 'Asia', 'Cohort Studies', 'Confidence Intervals', 'Emigration and Immigration', 'Europe', 'Female', 'Health Services Accessibility', 'Humans', 'Italy', 'Life Style', 'Obstetric Labor, Premature', 'Odds Ratio', 'Pregnancy', 'Pregnancy Outcome', 'Prenatal Care', 'Prospective Studies', 'Regression Analysis', 'Risk Factors', 'Travel', "Women's Health"]
| 18,666,924
|
[['M01.060.116'], ['Z01.058'], ['Z01.252'], ['E05.318.372.500.750', 'N05.715.360.330.500.750', 'N06.850.520.450.500.750'], ['E05.318.740.275', 'N05.715.360.750.220', 'N06.850.520.830.275'], ['I01.240.600.525.500', 'N01.224.625.525.500', 'N06.850.505.400.700.525.500'], ['Z01.542'], ['N04.590.374.350', 'N05.300.430'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['Z01.542.489'], ['F01.829.458'], ['C13.703.420.491'], ['E05.318.740.600.600', 'G17.680.500', 'N05.715.360.750.625.590', 'N06.850.520.830.600.600'], ['G08.686.784.769'], ['E01.789.700', 'G08.686.784.769.496'], ['E02.760.786', 'N02.421.143.620.704', 'N02.421.585.786'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['E05.318.740.750', 'N05.715.360.750.695', 'N06.850.520.830.750'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['I03.883'], ['N01.400.900']]
|
['Named Groups [M]', 'Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Organisms [B]', 'Psychiatry and Psychology [F]', 'Diseases [C]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 0
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 1
|
Development of interferon-specific monoclonal antibody for in vitro interferon assays.
|
In this study, monoclonal antibodies (MABs) to human interferon alpha (HuIFN alpha) were evaluated for their suitability for the quantification of different HuIFN alpha preparations by conventional immunoassay methods. The results obtained using four different MABs were compared and suggest that whilst immunoassays can be highly sensitive and reproducible to perform, calibration of antigen content in relation to units of biological activity remains a problem, particularly because of the molecular and biological heterogeneity of HuIFN alpha preparations. Nevertheless, the judicious selection of MABs specific for an individual HuIFN alpha subtype has indicated that immunoassays may be accurately calibrated in units of biological activity for potency estimations of that HuIFN alpha subtype.
|
['Antibodies, Monoclonal', 'Biological Assay', 'Enzyme-Linked Immunosorbent Assay', 'Humans', 'Immunoassay', 'Interferon Type I']
| 3,792,649
|
[['D12.776.124.486.485.114.224', 'D12.776.124.790.651.114.224', 'D12.776.377.715.548.114.224'], ['E05.091'], ['E05.478.566.350.170', 'E05.478.566.380.360', 'E05.478.583.400.170', 'E05.601.470.350.170', 'E05.601.470.380.360'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.478.566', 'E05.601.470'], ['D12.644.276.374.440.890', 'D12.776.467.374.440.890', 'D23.529.374.440.890']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Assignment of two loci for autosomal dominant adolescent idiopathic scoliosis to chromosomes 9q31.2-q34.2 and 17q25.3-qtel.
|
BACKGROUND: Adolescent idiopathic scoliosis (AIS) is the most common form of spinal deformity, affecting up to 4% of children worldwide. Familial inheritance of AIS is now recognised and several potential candidate loci have been found.METHODS: We studied 25 multi-generation AIS families of British descent with at least 3 affected members in each family. A genomewide screen was performed using microsatellite markers spanning approximately 10-cM intervals throughout the genome. This analysis revealed linkage to several candidate chromosomal regions throughout the genome. Two-point linkage analysis was performed in all families to evaluate candidate loci. After identification of candidate loci, two-point linkage analysis was performed in the 10 families that segregated, to further refine disease intervals.RESULTS: Significant linkage was obtained in a total of 10 families: 8 families to the telomeric region of chromosome 9q, and 2 families to the telomeric region of 17q. A significant LOD score was detected at marker D9S2157 Z(max) = 3.64 ( theta= 0.0) in a four-generation family (SC32). Saturation mapping of the 9q region in family SC32 defined the critical disease interval to be flanked by markers D9S930 and D9S1818, spanning approximately 21 Mb at 9q31.2-q34.2. In addition, seven other families segregated with this locus on 9q. In two multi-generation families (SC36 and SC23) not segregating with the 9q locus, a maximum combined LOD score of Z(max) = 4.08 ( = 0.0) was obtained for marker AAT095 on 17q. Fine mapping of the 17q candidate region defined the AIS critical region to be distal to marker D17S1806, spanning approximately 3.2 Mb on chromosome 17q25.3-qtel.CONCLUSION: This study reports a common locus for AIS in the British population, mapping to a refined interval on chromosome 9q31.2-q34.2 and defines a novel AIS locus on chromosome 17q25.3-qtel.
|
['Adolescent', 'Chromosome Mapping', 'Chromosomes, Human, Pair 17', 'Chromosomes, Human, Pair 9', 'Female', 'Genes, Dominant', 'Genotype', 'Humans', 'Lod Score', 'Male', 'Phenotype', 'Scoliosis']
| 17,932,119
|
[['M01.060.057'], ['E05.393.183'], ['A11.284.187.520.300.415.425', 'G05.360.162.520.300.415.425'], ['A11.284.187.520.300.325.345', 'G05.360.162.520.300.325.345'], ['G05.360.340.024.340.240', 'G05.420.320'], ['G05.380'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G05.348.750'], ['G05.695'], ['C05.116.900.800.875']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Diseases [C]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Roles of mechanistic target of rapamycin and transforming growth factor-â signaling in the molting gland (Y-organ) of the blackback land crab, Gecarcinus lateralis.
|
Molting in decapod crustaceans is controlled by molt-inhibiting hormone (MIH), an eyestalk neuropeptide that suppresses production of ecdysteroids by a pair of molting glands (Y-organs or YOs). Eyestalk ablation (ESA) activates the YOs, which hypertrophy and increase ecdysteroid secretion. At mid premolt, which occurs 7-14days post-ESA, the YO transitions to the committed state; hemolymph ecdysteroid titers increase further and the animal reaches ecdysis ~3weeks post-ESA. Two conserved signaling pathways, mechanistic target of rapamycin (mTOR) and transforming growth factor-â (TGF-â), are expressed in the Gecarcinus lateralis YO. Rapamycin, an mTOR antagonist, inhibits YO ecdysteroidogenesis in vitro. In this study, rapamycin lowered hemolymph ecdysteroid titer in ESA G. lateralis in vivo; levels were significantly lower than in control animals at all intervals (1-14days post-ESA). Injection of SB431542, an activin TGF-â receptor antagonist, lowered hemolymph ecdysteroid titers 7 and 14days post-ESA, but had no effect on ecdysteroid titers at 1 and 3days post-ESA. mRNA levels of mTOR signaling genes Gl-mTOR, Gl-Akt, and Gl-S6k were increased by 3days post-ESA; the increases in Gl-mTOR and Gl-Akt mRNA levels were blocked by SB431542. Gl-elongation factor 2 and Gl-Rheb mRNA levels were not affected by ESA, but SB431542 lowered mRNA levels at Days 3 and 7 post-ESA. The mRNA level of an activin TGF-â peptide, Gl-myostatin-like factor (Mstn), increased 5.5-fold from 0 to 3days post-ESA, followed by a 50-fold decrease from 3 to 7days post-ESA. These data suggest that (1) YO activation involves an up regulation of the mTOR signaling pathway; (2) mTOR is required for YO commitment; and (3) a Mstn-like factor mediates the transition of the YO from the activated to the committed state.
|
['Animals', 'Benzamides', 'Brachyura', 'Dioxoles', 'Ecdysteroids', 'Gene Expression Regulation', 'Hemolymph', 'Molting', 'Signal Transduction', 'Sirolimus', 'TOR Serine-Threonine Kinases', 'Transforming Growth Factor beta']
| 27,040,186
|
[['B01.050'], ['D02.065.277', 'D02.241.223.100.100', 'D02.455.426.559.389.127.085'], ['B01.050.500.131.365.190.110'], ['D03.383.246'], ['D04.210.500.247.222.265.165', 'D04.210.500.247.808.756.143', 'D06.472.445.573.271', 'D10.570.938.795.143', 'D23.704.500.143'], ['G05.308'], ['A13.453'], ['G07.345.500.550.750'], ['G02.111.820', 'G04.835'], ['D02.540.505.760'], ['D08.811.913.696.620.682.700.931', 'D12.776.476.925'], ['D12.644.276.374.687', 'D12.644.276.954.775', 'D12.776.467.374.687', 'D12.776.467.942.775', 'D23.529.374.687', 'D23.529.942.775']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
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Indigenous bacteria may interfere with the biocontrol of plant diseases.
|
Prodigiosin is a reddish antibiotic pigment that plays an important role in the biocontrol of plant diseases by the bacterium Serratia marcescens. However, its activity is unstable under agricultural conditions; further, it can be degraded by various environmental factors. To examine the effect of epiphytic microbes on the stability of prodigiosin used for biological control processes, we collected a total of 1,280 bacterial isolates from the phylloplane of cyclamen and tomato plants. Approximately 72% of the bacterial strains isolated from the cyclamen plants and 66% of those isolated from the tomato plants grew on minimal agar medium containing 100 microg ml(-1) prodigiosin. Certain isolates obtained from both plant species exhibited prodigiosin-degrading activity. We compared the 16S rRNA gene sequences derived from the isolates with sequences in a database. The comparison revealed that the sequences determined for the prodigiosin-degrading isolates were homologous to those of the genera Pseudomonas, Caulobacter, Rhizobium, Sphingomonas, Janthinobacterium, Novosphingobium, and Rathayibacter. These results indicate that indigenous epiphytic microorganisms may interfere with the interaction between plant pathogens and biocontrol agents by degrading the antibiotics produced by the agents.
|
['Bacteria', 'Bacterial Physiological Phenomena', 'Caulobacter', 'Cyclamen', 'DNA Primers', 'DNA, Bacterial', 'Plant Diseases', 'Plants', 'Prodigiosin', 'Pseudomonas', 'Rhizobium']
| 19,288,072
|
[['B03'], ['G06.099'], ['B03.440.400.280', 'B03.440.400.425.288.100', 'B03.660.050.090.100'], ['B01.650.940.800.575.912.250.341.984.549'], ['D13.695.578.424.450.275', 'D27.720.470.530.600.223.600'], ['D13.444.308.212'], ['G15.610'], ['B01.650'], ['D03.383.129.578.748', 'D23.767.778'], ['B03.440.400.425.625.625', 'B03.660.250.580.590'], ['B03.440.400.425.700.800', 'B03.585.900', 'B03.660.050.662.670']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Microcetus lappus gen. nov., sp. nov.: new species of ciliated protozoon from the bovine rumen.
|
A new species of small, ciliated protozoon, Microcetus lappus gen. nov., sp. nov., from the rumen of Norwegian Red cattle is described. M. lappus possesses a novel cytopharyngeal apparatus of two rod-shaped structures, one situated on the dorsal side of the buccal cavity and one on the ventral side, suggesting that it belongs to a previously undescribed taxon.
|
['Animals', 'Cattle', 'Ciliophora', 'Microscopy, Electron, Scanning', 'Rumen']
| 3,094,449
|
[['B01.050'], ['B01.050.150.900.649.313.500.380.271'], ['B01.043.185'], ['E01.370.350.515.402.541', 'E05.595.402.541'], ['A13.869.804']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]']
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
In vitro susceptibilities of Mycoplasma pneumoniae, Mycoplasma hominis, and Ureaplasma urealyticum to sparfloxacin and PD 127391.
|
The in vitro activities of two investigational quinolones, sparfloxacin (previously designated AT 4140) and PD 127391, were determined for 30 strains each of Mycoplasma pneumoniae, Mycoplasma hominis, and Ureaplasma urealyticum and compared with those of ciprofloxacin, tetracycline, clindamycin, and erythromycin. Erythromycin was the most active compound against M. pneumoniae (maximum MIC, less than 0.008 microgram/ml). PD 127391 (MICs, less than 0.008 to 0.031 microgram/ml), sparfloxacin (MICs, less than 0.008 to 0.25 microgram/ml), clindamycin (MICs, less than 0.008 to 0.5 microgram/ml), and tetracycline (MICs, 0.063 to 0.25 microgram/ml) were superior to ciprofloxacin (MICs, 0.5 to 2 microgram/ml). Sparfloxacin and PD 127391 were active against M. hominis (MICs, less than 0.008 to 0.031 microgram/ml for each) at concentrations comparable to those of clindamycin (MICs, less than 0.008 to 0.063 microgram/ml) and at concentrations lower than those of ciprofloxacin (MICs, 0.125 to 0.5 microgram/ml). As expected, M. hominis was resistant to erythromycin (MICs, 32 to greater than or equal to 256 micrograms/ml). For U. urealyticum, PD 127391 (MICs, 0.031 to 0.5 microgram/ml) and sparfloxacin (MICs, 0.063 to 1 microgram/ml) were superior to erythromycin (MICs, 0.25 to 4 micrograms/ml), ciprofloxacin (MICs, 0.5 to 8 micrograms/ml), and clindamycin (MICs, 0.25 to 64 micrograms/ml. Both new quinolones were equally active against tetracycline-susceptible as well as resistant strains of M. hominis and U. urealyticum. The possible influence of medium components and/or pH on MICs was evaluated by testing a Staphylococcus aureus reference strain with each antibiotic in SP-4 broth and 10-B broth and comparing the results with published MICs for this strain. MICs determined in 10-B broth for erythromycin were affected most. This study shows that the activities of sparfloxacin and PD 127391 are similar to one another and comparable or superior to those of other drugs used to treat mycoplasmal infections. The MICs of both new quinolones were consistently 2 to several dilutions lower than those of ciprofloxacin for each species.
|
['Anti-Infective Agents', 'Ciprofloxacin', 'Clindamycin', 'Culture Media', 'Erythromycin', 'Fluoroquinolones', 'Hydrogen-Ion Concentration', 'Microbial Sensitivity Tests', 'Mycoplasma', 'Mycoplasma pneumoniae', 'Tetracycline', 'Ureaplasma']
| 1,929,260
|
[['D27.505.954.122'], ['D03.633.100.810.835.322.186'], ['D03.383.773.532.500.125', 'D09.408.471.500.125'], ['D27.720.470.305', 'E07.206'], ['D02.540.576.500.992'], ['D03.633.100.810.835.322'], ['G02.300'], ['E01.370.225.875.595', 'E05.200.875.595', 'E05.337.550.400'], ['B03.440.860.580.553.553'], ['B03.440.860.580.553.553.650'], ['D02.455.426.559.847.562.900.875', 'D04.615.562.900.875'], ['B03.440.860.580.553.900']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Implementing the birth dose of hepatitis B vaccine in rural Indonesia.
|
Reaching mothers and their newborn infants around the time of birth with adequate health services has long been a difficult problem in developing countries. In parallel, similar problems have arisen in attempting to deliver hepatitis B (HepB) vaccine to infants born at home in many countries where mother-to-infant transmission is common. It is logical, and supported by experience in Indonesia, to find a combined solution for both problems. The World Health Organization (WHO) recommends that a timely birth dose of HepB vaccine be given, particularly in areas of high vertical transmission of hepatitis B virus (HBV). This can be achieved relatively easily in situations where almost all births occur in health facilities. But where a significant proportion of births occur at home and without birth attendants able to give injections, this is much more difficult. Barriers to the timely administration of the birth dose of HepB vaccine include weakness in policy development and implementation, difficulties in reliably supplying potent vaccine to community level, limited transport, poor communication, limited cold chain capacity, lack of effective training, and lack of a clear delineation of responsibility between health care professionals. Demonstration projects, such as those in Indonesia, suggest that there are significant opportunities to improve the timely delivery of HepB vaccine birth dose in existing maternal and child health programmes where health workers are trained to provide home delivery care.
|
['Female', 'Hepatitis B', 'Hepatitis B Vaccines', 'Humans', 'Immunization Programs', 'Indonesia', 'Infant, Newborn', 'Infectious Disease Transmission, Vertical', 'Pregnancy']
| 17,604,881
|
[['C01.221.250.500', 'C01.925.256.430.400', 'C01.925.440.435', 'C06.552.380.705.437'], ['D20.215.894.899.955.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['N02.421.726.608'], ['Z01.252.145.380', 'Z01.639.580'], ['M01.060.703.520'], ['N06.850.335.875'], ['G08.686.784.769']]
|
['Diseases [C]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Health Care [N]', 'Geographicals [Z]', 'Named Groups [M]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Identification of metallic-smelling 1-octen-3-one and 1-nonen-3-one from solutions of ferrous sulfate.
|
Taste threshold tests of ferrous sulfate (FeSO4) solutions have been confounded by the presence of putative odorants. To detect the presence of odorants released from these solutions solid-phase microextraction (SPME) was used to collect volatiles in the headspace above FeSO4 solutions. Gas chromatography-olfactometry of samples collected over three time periods (1, 5, and 16 h) and at two temperatures (22 and 37 degrees C) revealed the presence of several metallic-smelling odorants in the headspace. Using authentic standards, two of the odorants were conclusively identified as 1-octen-3-one and 1-nonen-3-one. Trace levels of other odorants were also detected, but dilution experiments indicated that 1-nonen-3-one was at least 10 times more potent than anything else released from the solutions. 1-Octen-3-one and 1-nonen-3-one are excellent candidates for the metallic odor responses often observed in threshold testing of solutions of FeSO4.
|
['Chromatography, Gas', 'Ferrous Compounds', 'Humans', 'Ketones', 'Odorants', 'Smell', 'Solutions']
| 16,218,683
|
[['E05.196.181.349'], ['D01.490.200'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D02.522'], ['G16.500.275.640', 'N06.230.480'], ['F02.830.816.643', 'G11.561.790.643'], ['D26.776']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Psychiatry and Psychology [F]']
| 0
| 1
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Correlation dimension maps of EEG from epileptic absences.
|
PURPOSE: The understanding of brain activity, and in particular events such as epileptic seizures, lies on the characterisation of the dynamics of the neural networks. The theory of non-linear dynamics provides signal analysis techniques which may give new information on the behaviour of such networks.METHODS: We calculated correlation dimension maps for 19-channel EEG data from 3 patients with a total of 7 absence seizures. The signals were analysed before, during and after the seizures. Phase randomised surrogate data was used to test chaos.RESULTS: In the seizures of two patients we could distinguish two dynamical regions on the cerebral cortex, one that seemed to exhibit chaos whereas the other seemed to exhibit noise. The pattern shown is essentially the same for seizures triggered by hyperventilation, but differ for seizures triggered by light flashes. The chaotic dynamics that one seems to observe is determined by a small number of variables and has low complexity. On the other hand, in the seizures of another patient no chaotic region was found. Before and during the seizures no chaos was found either, in all cases.CONCLUSIONS: The application of non-linear signal analysis revealed the existence of differences in the spatial dynamics associated to absence seizures. This may contribute to the understanding of those seizures and be of assistance in clinical diagnosis.
|
['Adolescent', 'Anticonvulsants', 'Brain Mapping', 'Child', 'Drug Resistance', 'Electroencephalography', 'Epilepsy, Absence', 'Female', 'Humans', 'Hyperventilation', 'Male', 'Photic Stimulation', 'Valproic Acid']
| 10,217,444
|
[['M01.060.057'], ['D27.505.954.427.080'], ['E01.370.350.578.875.500', 'E01.370.376.537.625.500', 'E05.629.875.500'], ['M01.060.406'], ['G07.690.773.984'], ['E01.370.376.300', 'E01.370.405.245'], ['C10.228.140.490.375.260', 'C10.228.140.490.493.125'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C08.618.501', 'C23.888.852.591'], ['E05.723.729'], ['D02.241.081.944.509.900', 'D10.251.400.895.593.900']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Characterization of the porcine peptidylarginine deiminase type VI gene (PADI6) promoter: Sp1 regulates basal transcription of the porcine PADI6.
|
It is a general consensus that oocyte quality is the key to embryo survival in pig reproduction. Thus, study on regulation of the ovary-associated gene is of great significance in pig breeding. Peptidylarginine deiminases (PADs) are a family of enzymes which catalyze the conversion of arginine to citrulline in proteins. The peptidylarginine deiminases type VI gene (PADI6) is mainly expressed in the ovary, and plays an important role in oocyte growth, fertilization and early embryo development. However, until now, little is known about its transcriptional regulation mechanism. Here, we firstly isolated and characterized the 5'-flanking region of porcine PADI6 gene. We determined the transcription start site using 5'-rapid amplification of cDNA ends (RACE) analysis, and identified the minimal promoter (-85/+68) that drove the basal expression of PADI6 by constructing various progressive deletions. Mutational analysis and electrophoretic mobility shift assays demonstrated Sp1 bound to the -56/-47 region of the PADI6 promoter. Furthermore, overexpression of Sp1 significantly increased the promoter activity and promoted PADI6 gene expression, and accordingly, inhibition of Sp1 expression with specific siRNA significantly reduced the promoter activity and suppressed the PADI6 expression. In addition, inhibition of Sp1 binding by Mithramycin A treatment reduced the transcriptional activity of PADI6 in a dose-dependent manner. Taken together, these data indicate that Sp1 is essential for the transcriptional regulation of PADI6.
|
['Animals', 'CHO Cells', 'Cricetulus', 'DNA Mutational Analysis', 'Female', 'Gene Expression Regulation', 'HeLa Cells', 'Humans', 'Hydrolases', 'Plicamycin', 'Promoter Regions, Genetic', 'Protein Binding', 'Sp1 Transcription Factor', 'Swine', 'Transcription, Genetic']
| 26,403,316
|
[['B01.050'], ['A11.251.210.200', 'A11.436.155'], ['B01.050.150.900.649.313.992.635.075.250.250'], ['E05.393.760.700.300'], ['G05.308'], ['A11.251.210.190.400', 'A11.251.860.180.400', 'A11.436.340'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D08.811.277'], ['D02.455.426.559.847.562.050.650', 'D04.615.562.050.650', 'D09.408.051.059.650'], ['G02.111.570.080.689.675', 'G05.360.080.689.675', 'G05.360.340.024.340.137.750.680'], ['G02.111.679', 'G03.808'], ['D12.776.260.522.750.249', 'D12.776.930.375.750.249'], ['B01.050.150.900.649.313.500.880'], ['G02.111.873', 'G05.297.700']]
|
['Organisms [B]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Increase in differentiated type of T lineage cells in the myasthenic thymus: two-color fluorocytometric analysis.
|
We made use of two-color flow cytometry to examine thymic lymphoid cells from patients with myasthenia gravis. The CD4+CD8- cells and the CD4-CD8+ cells were significantly increased in the thymus from the patients, compared with findings in the control samples. Conversely, the CD4+CD8+ cells were significantly decreased. Significant increases in CD1-CD3+ cells and significant decreases in CD1+CD3- cells were noted. The percentage of CD45R+ cells also increased. There were no increases in activated T lymphocytes, defined as CD4+DR+ cells, CD8+DR+ cells, or IL2R+ cells. We conclude that the proportion of T lineage lymphocytes that are differentiated is increased in the thymus of patients with myasthenia gravis, and could reflect accelerated differentiation.
|
['Adolescent', 'Adult', 'Antigens, Differentiation, T-Lymphocyte', 'CD4 Antigens', 'CD8 Antigens', 'Female', 'Flow Cytometry', 'Humans', 'Male', 'Middle Aged', 'Myasthenia Gravis', 'T-Lymphocytes', 'Thymus Gland']
| 2,113,791
|
[['M01.060.057'], ['M01.060.116'], ['D23.050.301.264.894', 'D23.101.100.894'], ['D12.776.543.750.705.852.420.810.500', 'D12.776.543.750.830.700.025', 'D23.050.301.264.894.100', 'D23.101.100.894.100'], ['D23.050.301.264.894.108', 'D23.101.100.894.108'], ['E01.370.225.500.363.342', 'E01.370.225.500.386.350', 'E05.196.712.516.600.240.350', 'E05.200.500.363.342', 'E05.200.500.386.350', 'E05.242.363.342', 'E05.242.386.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['C04.588.614.550.500', 'C04.730.856.490', 'C10.114.656', 'C10.574.781.588', 'C10.668.758.725', 'C20.111.258.500'], ['A11.118.637.555.567.569', 'A15.145.229.637.555.567.569', 'A15.382.490.555.567.569'], ['A10.549.750', 'A15.382.520.604.750']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Diseases [C]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Reconceptualizing successful aging among black women and the relevance of the strong black woman archetype.
|
Although there are multiple pathways to successful aging, little is known of what it means to age successfully among black women. There is a growing body of literature suggesting that black women experience a number of social challenges (sexism and racism) that may present as barriers to aging successfully. Applying aspects of the Strong Black Women ideal, into theoretical concepts of successful aging, may be particularly relevant in understanding which factors impair or promote the ability of black women to age successfully. The Strong Black Women archetype is a culturally salient ideal prescribing that black women render a guise of self-reliance, selflessness, and psychological, emotional, and physical strength. Although this ideal has received considerable attention in the behavioral sciences, it has been largely absent within the gerontology field. Nevertheless, understanding the dynamics of this cultural ideal may enhance our knowledge while developing an appreciation of the black woman's ability to age successfully. Rather than summarize the social, physical, and mental health literature focusing on health outcomes of black women, this conceptual review examines the Strong Black Women archetype and its application to the lived experiences of black women and contributions to current theories of successful aging. Focusing on successful aging exclusively among black women enhances our understanding of this group by considering their identity as women of color while recognizing factors that dictate their ability to age successfully.
|
['Adaptation, Psychological', 'Adult', 'African Americans', 'African Continental Ancestry Group', 'Aging', 'Female', 'Geriatrics', 'Health Status', 'Humans', 'Mental Disorders', 'Mental Health', 'Quality of Life', 'Resilience, Psychological']
| 25,416,685
|
[['F01.058'], ['M01.060.116'], ['M01.686.508.100.100', 'M01.686.754.100'], ['M01.686.508.100'], ['G07.345.124'], ['H02.403.355'], ['I01.240.425', 'N01.224.425', 'N06.850.505.400.425'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F03'], ['F02.418', 'N01.400.500'], ['I01.800', 'K01.752.400.750', 'N06.850.505.400.425.837'], ['F02.940']]
|
['Psychiatry and Psychology [F]', 'Named Groups [M]', 'Phenomena and Processes [G]', 'Disciplines and Occupations [H]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]', 'Organisms [B]', 'Humanities [K]']
| 0
| 1
| 0
| 0
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 1
| 0
|
Somatostatin receptor subtypes in human type 2 diabetic islets.
|
OBJECTIVES: Somatostatin inhibits hormone release through 5 G protein-coupled somatostatin receptors (sst1-sst5). However, the role of somatostatin in islet physiology is not fully known. The immunoreactivity to sst1 to sst5 in normal human endocrine pancreas has been described. The present study reports the expression of sst1 to sst5 in human pancreatic islets with type 2 diabetes mellitus.METHODS: Pancreatic autopsy specimens from individuals with type 2 diabetes mellitus and matched controls were double immunostained to demonstrate sst1 to sst5 in the major islet cell types.RESULTS: Most apparent differences in type 2 diabetic islets were the lack of sst1 and sst4 in glucagon cells and sst1-3 and 4 in somatostatin cells, whereas minor changes were demonstrated in insulin cells. The pancreatic polypeptide cells showed a reversed staining pattern in diabetic islets compared with the controls.CONCLUSIONS: In type 2 diabetes mellitus, the sst pattern differed from that of the controls in somatostatin, pancreatic polypeptide, and glucagon cells, to a minor extent in insulin cells. It is unclear whether the changes in sst patterns are primarily due to the diabetes or secondary to metabolic disturbances. However, this study may be the basis for further functional studies to evaluate the role of sst1 to sst5 in the diabetic state.
|
['Aged', 'Aged, 80 and over', 'Autopsy', 'Diabetes Mellitus, Type 2', 'Female', 'Humans', 'Immunohistochemistry', 'Islets of Langerhans', 'Male', 'Pancreatic Polypeptide-Secreting Cells', 'Protein Isoforms', 'Receptors, Somatostatin', 'Somatostatin-Secreting Cells']
| 20,182,388
|
[['M01.060.116.100'], ['M01.060.116.100.080'], ['E01.370.060', 'E05.070', 'I01.198.780.937.120'], ['C18.452.394.750.149', 'C19.246.300'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.225.500.607.512', 'E01.370.225.750.551.512', 'E05.200.500.607.512', 'E05.200.750.551.512', 'E05.478.583', 'H01.158.100.656.234.512', 'H01.158.201.344.512', 'H01.158.201.486.512', 'H01.181.122.573.512', 'H01.181.122.605.512'], ['A03.734.414', 'A06.300.414'], ['A03.734.414.587', 'A06.300.414.587', 'A06.390.650', 'A11.382.625.900', 'A11.436.294.900'], ['D12.776.800'], ['D12.776.543.750.695.850', 'D12.776.543.750.720.600.760', 'D12.776.543.750.750.555.760', 'D12.776.543.750.750.580.720', 'D12.776.543.750.750.700.800'], ['A03.556.875.875.440.854', 'A03.734.414.793', 'A06.300.414.793', 'A06.390.825', 'A10.615.550.291.825', 'A11.382.625.950', 'A11.436.294.950']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Diseases [C]', 'Organisms [B]', 'Disciplines and Occupations [H]', 'Anatomy [A]', 'Chemicals and Drugs [D]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
|
Treatment of respiratory failure with inhaled nitric oxide and high- frequency ventilation in an infant with respiratory syncytial virus pneumonia and bronchopulmonary dysplasia.
|
In a 7-month-old infant with bronchopulmonary dysplasia and respiratory syncytial virus (RSV) pneumonia, we have shown an additive effect of high-frequency ventilation (HFV) and inhaled nitric oxide (iNO) in terms of improved oxygenation and the avoidance of extracorporeal membrane oxygenation. Apparently, the combined therapy of HFV and iNO is superior to either therapeutic modality alone in the treatment of hypoxemic respiratory failure due to RSV pneumonia. The mechanism of increased lung expansion and alveolar recruitment appears to be responsible for a favorable clinical outcome. We conclude that the combined therapy of HFV and iNO should be considered in hypoxemic respiratory failure in pediatric patients.
|
['Administration, Inhalation', 'Bronchopulmonary Dysplasia', 'High-Frequency Ventilation', 'Humans', 'Infant', 'Infant, Newborn', 'Nitric Oxide', 'Pneumonia, Viral', 'Respiratory Insufficiency', 'Respiratory Syncytial Virus Infections', 'Treatment Outcome']
| 9,817,963
|
[['E02.319.267.050'], ['C08.381.520.750.500', 'C16.614.521.125'], ['E02.041.625.508', 'E02.880.820.508'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703'], ['M01.060.703.520'], ['D01.339.387', 'D01.625.550.500', 'D01.625.700.500', 'D01.650.550.587.600'], ['C01.748.610.763', 'C01.925.705', 'C08.381.677.807', 'C08.730.610.763'], ['C08.618.846'], ['C01.925.782.580.600.550.750'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Organisms [B]', 'Named Groups [M]', 'Chemicals and Drugs [D]', 'Health Care [N]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Airway foreign bodies in pediatric patients: anatomic location of foreign body affects complications and outcomes.
|
BACKGROUND: Airway foreign bodies (FB) are a common medical emergency within the pediatric population. While deaths are not uncommon, the in-hospital mortality rates and correlation with anatomic location of the airway foreign body have not been previously reported.METHODS: The KID database was reviewed for 2003, 2006, 2009, and 2012 for pediatric patients with a discharge diagnosis of airway foreign body using ICD-9 codes (933.1, 934.x).RESULTS: 11,793 patients, ages 0-17, were found to have an airway FB. Of patients admitted for airway FB 21.2 % required mechanical ventilation during their hospitalization, and the overall mortality rate was 2.5 %. Location of the airway FB was dependent on age (p < 0.01). Use of mechanical ventilation was dependent on the location of the airway FB (p < 0.01) and being transferred from another hospital (OR 2.59, p < 0.01). Univariate analysis demonstrated differences in in-hospital mortality based on location (p < 0.01), use of a ventilator during hospitalization (OR 24.4, p < 0.01), and transfer from another hospital (OR 2.11, p < 0.01).CONCLUSIONS: The in-hospital mortality rate for airway foreign bodies is 2.5 %. The anatomic location of airway FB in pediatric patients varies by age, and affects the need for mechanical ventilation and in-hospital mortality.
|
['Adolescent', 'Airway Obstruction', 'Bronchoscopy', 'Child', 'Child, Preschool', 'Female', 'Foreign Bodies', 'Hospital Mortality', 'Humans', 'Incidence', 'Infant', 'Infant, Newborn', 'Male', 'Respiratory System', 'Retrospective Studies', 'United States']
| 27,738,825
|
[['M01.060.057'], ['C08.618.846.185'], ['E01.370.386.105', 'E01.370.388.250.100', 'E04.502.250.100', 'E04.928.600.080'], ['M01.060.406'], ['M01.060.406.448'], ['C26.392'], ['E05.318.308.985.550.400', 'N01.224.935.698.400', 'N06.850.505.400.975.550.400', 'N06.850.520.308.985.550.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.308.985.525.375', 'N01.224.935.597.500', 'N06.850.505.400.975.525.375', 'N06.850.520.308.985.525.375'], ['M01.060.703'], ['M01.060.703.520'], ['A04'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['Z01.107.567.875']]
|
['Named Groups [M]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Anatomy [A]', 'Geographicals [Z]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Individual electrophoretic mobilities of liposomes and acidic organelles displaying pH gradients across their membranes.
|
This report focuses on measuring the individual electrophoretic mobilities of liposomes with different pH gradients across their membrane using capillary electrophoresis with laser-induced fluorescence detection (CE-LIF). The results from the individual analysis of liposomes show that, using surface electrostatic theories and the electrokinetic theory as the first approximation, zeta potential contributes more significantly to the electrophoretic mobility of liposomes than liposomal size. For liposomes with an outer pH 7.4 (pH(o) 7.4) and a net negative outer surface charge, the most negative electrophoretic mobilities occur when the inner pH (pH(i)) is 6.8; at higher or lower pH(i), the electrophoretic mobilities are less negative. The theories mentioned above cannot explain these pH-induced electrophoretic mobility shifts. The capacity theory, predicting an induced electrical charge on the surface of liposomes, can only explain the results at pH(i) > 6.8. In this report, we hypothesize that there is a flip-flop process of phospholipids, which refers to the exchange of phospholipids between the outer and inner layers of the membrane. This flip-flop is caused by the pH gradient and membrane instability and results in the observed electrophoretic mobility changes when pH(i) is <6.8. Furthermore, it is found that the mobilities of acidic organelles are consistent with the predictions of liposome models we used here.
|
['Animals', 'Cell Line', 'Electrophoresis', 'Hydrogen-Ion Concentration', 'Liposomes', 'Models, Chemical', 'Organelles', 'Phospholipids']
| 17,402,758
|
[['B01.050'], ['A11.251.210'], ['E05.196.401', 'E05.301.300'], ['G02.300'], ['D25.479.517', 'D26.255.260.517', 'J01.637.051.479.517', 'J01.637.087.500.517'], ['E05.599.495'], ['A11.284.430.214.190.875'], ['D10.570.755']]
|
['Organisms [B]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Technology, Industry, and Agriculture [J]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Protease activation and the signal transduction pathway regulating motility in sperm from the water strider Aquarius remigis.
|
Many motile processes are regulated such that movement occurs only upon activation of a signaling cascade. Sperm from a variety of species are initially quiescent and must be activated prior to beating. The signaling events leading to the activation and regulation of sperm motility are not well characterized. Mature seminal vesicle sperm from the water strider Aquarius remigis are immotile in vitro, but vigorous motility is activated by trypsin. Trypsin-activated motility was blocked by pretreatment of the sperm with BAPTA-AM to chelate intracellular Ca(2+) and was partially rescued by subsequent addition of A23187 and Ca(2+). Thapsigargin stimulated motility in the absence of trypsin, suggesting that intracellular Ca(2+) stores are available. In addition, motility could be fully activated by the phosphatase inhibitor calyculin A, suggesting that the immotile state is maintained by an endogenous phosphatase and that kinase activity is required for motility. The MEK1/2 inhibitor U0126 significantly reduced trypsin activated motility, and MPM-2, an antibody which recognizes proline-directed phosphorylation by kinases such as MAPK, recognized components of the water strider sperm flagellum. Antibodies specific for the mouse protease activated receptor PAR2 recognized an antigen on the sperm flagellum. These results suggest that trypsin stimulates a Ca(2+) and MAPK mediated signaling pathway and potentially implicate a PAR2-like protein in regulating motility.
|
['Animals', 'Calcium', 'Egtazic Acid', 'Heteroptera', 'MAP Kinase Signaling System', 'Male', 'Marine Toxins', 'Oxazoles', 'Phosphorylation', 'Protein Kinase Inhibitors', 'Protein Kinases', 'Receptor, PAR-2', 'Signal Transduction', 'Sperm Motility', 'Spermatozoa', 'Staurosporine', 'Thapsigargin', 'Trypsin']
| 22,278,949
|
[['B01.050'], ['D01.268.552.100', 'D01.552.539.288', 'D23.119.100'], ['D02.092.782.258.368.257', 'D02.241.081.018.269'], ['B01.050.500.131.617.412.420'], ['G02.111.820.560', 'G03.493.560', 'G04.835.560'], ['D23.946.580'], ['D03.383.129.462'], ['G02.111.665', 'G02.607.780', 'G03.796'], ['D27.505.519.389.755'], ['D08.811.913.696.620.682'], ['D12.776.543.750.695.035', 'D12.776.543.750.792.249'], ['G02.111.820', 'G04.835'], ['E01.370.225.992.812', 'E05.200.992.812', 'G04.198.750'], ['A05.360.490.890', 'A11.497.760'], ['D03.132.436.750', 'D03.633.100.473.144.750', 'D03.633.100.473.402.750', 'D03.633.100.496.500.500.750', 'D03.633.300.148.750'], ['D02.455.426.392.368.284.500.888', 'D02.455.849.765.674.500.750.888', 'D04.663.500.750.888'], ['D08.811.277.656.300.760.895', 'D08.811.277.656.959.350.895']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
The application of in vivo laser scanning confocal microscopy as a tool of conjunctival in vivo cytology in the diagnosis of dry eye ocular surface disease.
|
PURPOSE: To evaluate the applicability of in vivo laser scanning confocal microscopy as a tool of conjunctival cytology in a prospective case-control study.METHODS: Nineteen right eyes of 19 Sjogren's syndrome dry eye patients (19 females; mean age: 55.8±15 years), and 18 right eyes of 18 normal healthy control subjects (12 females and 6 males; mean age: 50.8±14 years) were evaluated in this study. The eyes were analyzed by the Heidelberg retina tomography (HRTII)/Rostock cornea module (RCM). Ocular surface and tear function tests including vital stainings (fluorescein and Rose Bengal), Schirmer test, tear film break up time (BUT), and conjunctival impression cytology were performed. After obtaining the confocal microscopy images, the mean individual epithelial cell area (MIECA), and nucleocytoplasmic (N/C) ratio were analyzed. The correlation between confocal microscopy and impression cytology parameters was also investigated.RESULTS: The BUT, Schirmer test values, vital staining scores and squamous metaplasia grades in impression cytology were significantly worse in dry eye patients compared to controls (p<0.0001). The MIECA and the mean N/C ratios were worse in dry eye subjects compared to controls both in impression cytology and in vivo confocal microscopy (p<0.0001) with no significant differences between these parameters when the two examination techniques were compared. The MIECA and N/C ratio in conjunctival impression cytology showed significant correlation with the corresponding confocal microscopy parameters (MIECA, r2:0.557 ; N/C, r2:0.765).CONCLUSIONS: Laser scanning confocal microscopy seems to be an efficient non-invasive tool in the evaluation of phenotypic alterations of the conjunctival epithelium in dry eye disease. N/C ratio and MIECA appear to be two promising and new parameters of in vivo confocal cytology in the assessment of the ocular surface in dry eye disease.
|
['Case-Control Studies', 'Cell Nucleus', 'Conjunctiva', 'Cytological Techniques', 'Epithelial Cells', 'Female', 'Humans', 'Male', 'Microscopy, Confocal', 'Middle Aged', "Sjogren's Syndrome", 'Tears']
| 21,139,693
|
[['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['A11.284.430.106', 'A11.284.430.214.190.875.117'], ['A09.371.060.200', 'A09.371.337.168'], ['E01.370.225.500', 'E05.200.500', 'E05.242'], ['A11.436'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.350.515.395', 'E05.595.395'], ['M01.060.116.630'], ['C05.550.114.154.774', 'C05.799.114.774', 'C07.465.815.929.669', 'C11.496.260.719', 'C17.300.775.099.774', 'C20.111.199.774'], ['A12.200.882']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Anatomy [A]', 'Organisms [B]', 'Named Groups [M]', 'Diseases [C]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Reprogramming of Vibrio harveyi gene expression during adaptation in cold seawater.
|
The life and survival of the marine bacterium Vibrio harveyi during its adaptation in natural aquatic systems is highly influenced by the availability of nutrients and temperature. To learn about adaptation strategies evolved by this bacterium to cope with drastic temperature downshifts and nutrients depletion, we have studied the phenotypical and gene expression changes occurring in V. harveyi during its adaptation to cold seawater. We found that incubation in cold seawater up to 12 h did not cause any significant morphological changes in V. harveyi and had no effect on the number of viable and culturable cells. Microarray analysis revealed that the V. harveyi response to cold seawater leads to up- and downregulation of numerous genes controlling the central carbon metabolism, nucleotide and amino acid biosynthesis as well as DNA repair. In addition, expression of some genes controlling biosynthesis of lipids, molecular transport, and energy production was altered to likely affect the composition and properties of the V. harveyi cell envelope, thus implying the putative role of this compartment in adaptation to stress. Here, we discuss these results with regard to the putative adaptive responses likely triggered by V. harveyi to cope with environmental challenges in natural aquatic systems.
|
['Adaptation, Physiological', 'Cold Temperature', 'Gene Expression', 'Gene Expression Regulation, Bacterial', 'Seawater', 'Vibrio']
| 24,102,529
|
[['G07.025', 'G16.012.500'], ['G01.906.595.272', 'G16.500.275.063.725.710.300', 'G16.500.750.775.710.300', 'N06.230.300.100.725.154', 'N06.230.300.100.725.710.300'], ['G05.297'], ['G05.308.300'], ['G16.500.275.725.500'], ['B03.440.450.900.859', 'B03.660.250.830.830']]
|
['Phenomena and Processes [G]', 'Health Care [N]', 'Organisms [B]']
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Affinity of cefmenoxime for beta-lactamases: an analysis.
|
The interactions of cefmenoxime with beta-lactamases in comparison with cefotaxime, moxalactam, cefoperazone, and ceftazidime have been determined. On-line computerized microacidimetry allowed determination of the affinity of these compounds with the enzymes, which was characterized by Km values. The beta-lactamases that were used were two cephalosporinases and one penicillinase. Within these data, the cephalosporins could be classified into three groups: (1) those with high affinity for the cephalosporinases and very poor affinity for the penicillinase (cefmenoxime, cefotaxime, and moxalactam); (2) those with moderate affinity for the cephalosporinases and very poor affinity for the penicillinase (ceftazidime); (3) those with poor affinity for all enzymes (cefoperazone). In the case of the penicillinase (TEM-1), only cefoperazone was subject to some hydrolysis.
|
['Bacteria', 'Cefmenoxime', 'Cefotaxime', 'Cephalosporinase', 'Cephalosporins', 'Hydrolysis', 'Kinetics', 'Moxalactam', 'Penicillinase', 'beta-Lactamases']
| 6,097,119
|
[['B03'], ['D02.065.589.099.249.190.190.125', 'D02.886.665.074.190.190.125', 'D03.633.100.300.249.190.190.125'], ['D02.065.589.099.249.190.190', 'D02.886.665.074.190.190', 'D03.633.100.300.249.190.190'], ['D08.811.277.087.180.229'], ['D02.065.589.099.249', 'D02.886.665.074', 'D03.633.100.300.249'], ['G02.380'], ['G01.374.661', 'G02.111.490'], ['D02.065.589.099.625', 'D02.886.520.575', 'D03.633.100.300.625'], ['D08.811.277.087.180.697'], ['D08.811.277.087.180']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
E. Graeme Robertson--dynamics in fluid and light.
|
An eponymous lecture at the Australian and New Zealand Association of Neurologists Annual Scientific Meeting commemorates E. Graeme Robertson (1903-75), and some neurologists will know that particular Australian practices in clinical neurology, so far as they exist, have origins in his career. This is a historical article on the literary record of a man who had his own sense of history--an affinity with the past as well as an awareness of future generations of readers. He wrote authoritative texts on pneumoencephalography before new technology made it obsolete, and he produced a series of books on decorative architectural cast iron in Australian cities. A talent for visual interpretation seems to have drawn him to both of these topics; a common theme is contrast between light and dark, which is expatiated in images and in clear, well-written prose in his publications. We review his medical writings, including some largely forgotten principles of cerebrospinal fluid physics that he discovered when researching pneumoencephalography. We also explore his obsession with cast iron--its architectural historical significance, his techniques for photographing it, and some of the ways that it related to his life's work as a clinical neurologist.
|
['Architecture', 'Australia', 'History, 20th Century', 'Humans', 'Nervous System Diseases', 'Neurology', 'Pneumoencephalography']
| 23,151,435
|
[['J01.086'], ['Z01.639.100', 'Z01.678.100.373'], ['K01.400.504.968'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C10'], ['H02.403.600'], ['E01.370.350.578.937.620', 'E01.370.350.700.560.620', 'E01.370.350.700.625.620', 'E01.370.376.537.750.620', 'E05.629.937.620']]
|
['Technology, Industry, and Agriculture [J]', 'Geographicals [Z]', 'Humanities [K]', 'Organisms [B]', 'Diseases [C]', 'Disciplines and Occupations [H]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
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| 1
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Isoflavanones from the heartwood of Swartzia polyphylla and their antibacterial activity against cariogenic bacteria.
|
The methanolic extract of Swartzia polyphylla DC. heartwood had antibacterial activity against cariogenic bacteria, the mutans Streptococci. The chromatographic purification of the extract afforded seven flavonoids. Among them, three known isoflavanones, dihydrobiochanin A, ferreirin and darbergioidin, and one new isoflavanone, 5,2',4'-trihydroxy-7-methoxyisoflavanone (dihydrocajanin) had potent antibacterial activity against cariogenic bacteria. This effect was not detected on isoflavone derivatives. A comparative antibacterial study of various flavonoids was further performed, and their structure-activity relationship was discussed.
|
['Anti-Bacterial Agents', 'Chromatography, High Pressure Liquid', 'Circular Dichroism', 'Culture Media', 'Flavonoids', 'Isoflavones', 'Microbial Sensitivity Tests', 'Streptococcus', 'Structure-Activity Relationship', 'Trees']
| 1,477,911
|
[['D27.505.954.122.085'], ['E05.196.181.400.300'], ['E05.196.867.151'], ['D27.720.470.305', 'E07.206'], ['D03.383.663.283.266.450', 'D03.633.100.150.266.450'], ['D03.383.663.283.266.450.400', 'D03.633.100.150.266.450.400'], ['E01.370.225.875.595', 'E05.200.875.595', 'E05.337.550.400'], ['B03.353.750.737.872', 'B03.510.400.800.872', 'B03.510.550.737.872'], ['G02.111.830', 'G07.690.773.997'], ['B01.650.915']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
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Interactions of insulin-mimetic zinc(II) complexes with cell constituents: glutathione and ATP.
|
Ternary complex formation of some potent insulin-mimetic zinc(II) complexes of bidentate ligands: maltol and 3-hydroxy-1,2-dimethyl-pyridinone with (O,O), 2-picolinic acid and 6-methylpicolinic acid with (N,O) and the tridentate 2,6-dipicolinic acid with (O,N,O) coordination modes was studied in aqueous solutions by pH-potentiometry and spectroscopic (UV, CD, ESI-MS) methods in the presence of critical cell constituents such as L-glutathione reduced (GSH) and adenosine 5'-triphosphate (ATP). Results showed that formation of the ternary complexes was hindered in the case of 2,6-dipicolinic acid, especially with ATP, while it was favoured with the bidentate ligands in the physiological pH range. Driving force of the formation of mixed-ligand species was found to be a more enhanced coordination of GSH and ATP as second ligands in the ternary complexes than in their binary ones due to steric and electrostatic reasons. The mitochondrial dehydrogenase activity of the zinc(II) complexes, as an indirect indicator for the glucose intake, was measured on Mono Mac and 3T3-L1 adipocyte cell lines. The activity of the complexes up to approximately 10-100 microM concentration was in the range of the effect of 0.75-1.5 microM insulin, while at higher concentration it was broken down due to the sensitivity of the cells to toxicity of the complexes.
|
['3T3-L1 Cells', 'Adenosine Triphosphate', 'Animals', 'Biomimetic Materials', 'Glutathione', 'Insulin', 'Ligands', 'Mice', 'Organometallic Compounds', 'Oxidoreductases', 'Potentiometry', 'Spectrum Analysis', 'Zinc']
| 18,282,604
|
[['A11.251.210.100.775.800', 'A11.329.228.100.775.800'], ['D03.633.100.759.646.138.236', 'D13.695.667.138.236', 'D13.695.827.068.236'], ['B01.050'], ['J01.637.087'], ['D12.644.456.448'], ['D06.472.699.587.200.500.625', 'D12.644.548.586.200.500.625'], ['D27.720.470.480'], ['B01.050.150.900.649.313.992.635.505.500'], ['D02.691'], ['D08.811.682'], ['E05.196.922.750', 'E05.301.710'], ['E05.196.867'], ['D01.268.556.940', 'D01.268.956.906', 'D01.552.544.940']]
|
['Anatomy [A]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Technology, Industry, and Agriculture [J]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Ultrasensitive detection of bacteria by microchip electrophoresis based on multiple-concentration approaches combining chitosan sweeping, field-amplified sample stacking, and reversed-field stacking.
|
In this paper we describe an on-chip multiple-concentration method combining chitosan (CS) sweeping, reversed-field stacking, and field-amplified sample stacking for highly efficient detection of bacteria. Escherichia coli was selected as a model bacterium to investigate the efficiency of this multiple-concentration method. CS was the most suitable sweeping agent for microchip electrophoresis, replacing the usually used cetyltrimethylammonium bromide for capillary electrophoresis. The additive taurine had a synergistic effect by enhancing the interaction between CS and the surface of the bacteria, thus improving the analysis sensitivity. All steps of the concentration method and related mechanisms are described and discussed in detail. A concentration enhancement factor of approximately 6000 was obtained using this concentration method under optimal conditions as compared to using no concentration step, and the detection limit of E. coli was 145 CFU/mL. The multiple-concentration methodology was also applied for the quantification of bacteria in surface water, and satisfactory results were achieved. The application of this methodology showed that the concentration enhancement of bacteria clearly conferred advantageous sensitivity, speed, and sample volume compared to established methods.
|
['Cetrimonium', 'Cetrimonium Compounds', 'Chitosan', 'Electrophoresis, Microchip', 'Escherichia coli', 'Water Microbiology']
| 22,242,961
|
[['D02.092.877.883.111.500', 'D02.675.276.190.500'], ['D02.092.877.883.111', 'D02.675.276.190'], ['D05.750.078.139.500', 'D09.698.211.500'], ['E05.196.401.190.500', 'E05.588.465.340'], ['B03.440.450.425.325.300', 'B03.660.250.150.180.100'], ['H01.158.273.540.274.777', 'N06.850.425.450']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Disciplines and Occupations [H]', 'Health Care [N]']
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
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Preliminary characterization, androgen-dependence and ontogeny of an abundant protein from mouse vas deferens.
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Polyacrylamide gel electrophoresis analysis revealed that the vas deferens of adult mouse contains a major protein. Mouse vas deferens protein is a basic glycoprotein with a molecular weight of 34,800 +/- 300. The protein represents 17 +/- 0.7% and 42 +/- 2.4% of soluble proteins from homogenate and luminal fluid respectively, an estimate based on densitometric scanning of polyacrylamide gels. The protein originated from the vas deferens since it was not detected in blood plasma or in sexual organs and it was still present after ligation of the epididymis. Changes in androgen status of the animal markedly affected the vas deferens protein. After castration a progressive decrease in the protein was observed and its relative percentage dropped to 2 +/- 0.4% after 45 days. The concentration of the protein returned to precastration levels after 2 weeks of testosterone treatment but oestradiol, progesterone and corticosterone were ineffective in this respect. The vas deferens protein was not synthesized in significant amounts until animals were 20 days old and its concentration increased rapidly from 20 to 30 days in concert with the pubertal increase of androgens in the vas deferens.
|
['Animals', 'Electrophoresis, Polyacrylamide Gel', 'Glycoproteins', 'Male', 'Mice', 'Mice, Inbred Strains', 'Orchiectomy', 'Sexual Maturation', 'Vas Deferens']
| 3,411,574
|
[['B01.050'], ['E05.196.401.402', 'E05.301.300.319'], ['D09.400.430', 'D12.776.395'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520', 'B01.050.150.900.649.313.992.635.505.500.400'], ['E04.270.282.679', 'E04.950.165.679', 'E04.950.774.860.618'], ['G07.345.750.750', 'G08.686.841.750'], ['A05.360.444.930']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
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Phytochrome from Agrobacterium tumefaciens has unusual spectral properties and reveals an N-terminal chromophore attachment site.
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Phytochromes are photochromic photoreceptors with a bilin chromophore that are found in plants and bacteria. The soil bacterium Agrobacterium tumefaciens contains two genes that code for phytochrome-homologous proteins, termed Agrobacterium phytochrome 1 and 2 (Agp1 and Agp2). To analyze its biochemical and spectral properties, Agp1 was purified from the clone of an E. coli overexpressor. The protein was assembled with the chromophores phycocyanobilin and biliverdin, which is the putative natural chromophore, to photoactive holoprotein species. Like other bacterial phytochromes, Agp1 acts as light-regulated His kinase. The biliverdin adduct of Agp1 represents a previously uncharacterized type of phytochrome photoreceptor, because photoreversion from the far-red absorbing form to the red-absorbing form is very inefficient, a feature that is combined with a rapid dark reversion. Biliverdin bound covalently to the protein; blocking experiments and site-directed mutagenesis identified a Cys at position 20 as the binding site. This particular position is outside the region where plant and some cyanobacterial phytochromes attach their chromophore and thus represents a previously uncharacterized binding site. Sequence comparisons imply that the region around Cys-20 is a ring D binding motif in phytochromes.
|
['Agrobacterium tumefaciens', 'Amino Acid Sequence', 'Base Sequence', 'Binding Sites', 'DNA Primers', 'Molecular Sequence Data', 'Molecular Structure', 'Mutation', 'Phosphorylation', 'Phytochrome', 'Sequence Homology, Amino Acid']
| 12,186,972
|
[['B03.440.400.425.700.024.050', 'B03.660.050.662.024.500'], ['G02.111.570.060', 'L01.453.245.667.060'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['G02.111.570.120'], ['D13.695.578.424.450.275', 'D27.720.470.530.600.223.600'], ['L01.453.245.667'], ['G02.111.570', 'G02.466'], ['G05.365.590'], ['G02.111.665', 'G02.607.780', 'G03.796'], ['D12.776.765.675', 'D23.767.712'], ['G02.111.810.200', 'G05.810.200']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Chemicals and Drugs [D]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 0
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Phase II trial of oxaliplatin and tegafur/uracil and oral folinic acid for advanced or metastatic colorectal cancer in elderly patients.
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OBJECTIVES: Colorectal cancer is usually diagnosed in elderly patients. Since there is clear evidence that such patients are under-treated and under-represented or even excluded from clinical studies and there are no reliable and prospective data on the feasibility and efficacy of an oxaliplatin (L-OHP)-based chemotherapy in this setting, we have tested the L-OHP plus oral uracil/tegafur (UFT) and oral folinic acid (FA) combination as first-line therapy in patients with advanced or metastatic colorectal cancer (MCRC) aged 70 or older.PATIENTS AND METHODS: Forty-seven patients with advanced or MCRC, aged over 70, were treated with L-OHP 65 mg/m(2) as an intravenous 3-hour infusion on day 1 and 8 plus UFT 300 mg/m(2) and FA 90 mg in 3 divided doses given orally on days 1-14 for each 3-week cycle. Patients were followed by a geriatric and a quality of life (QoL) assessment with specific scales and EORTC-QLQ-C30 questionnaire.RESULTS: All patients were assessable for toxicity and 45 for response to treatment. Complete response was achieved in 2 patients (4%) and partial response in 22 (47%) [overall response rate, 51%; 95% confidence interval (CI): 40.7-61.2%]; 18 patients (38%) had stable disease, and 5 (11%) had disease progression. The median duration of response was 8 months (range, 3-19+ months). After a minimum follow-up of 17 months, the median time to disease progression and the median overall survival were 8.0 (95% CI: 6.7-9.3%) and 14.1 (95% CI: 11.0-17.1%) months, respectively. Regimen safety was manageable. Most adverse events were mild to moderate, and this did not result in QoL impairment. The most common grade 3-4 treatment-related adverse events were diarrhea (17%), neutro- and thrombocytopenia (2%), laryngeal spasm (2%), and peripheral neuropathy (12.7%). No treatment-related deaths occurred.CONCLUSIONS: These results confirmed that this tested chemotherapy combination is active with acceptable tolerability and QoL maintenance in elderly patients with advanced or MCRC.
|
['Administration, Oral', 'Age Factors', 'Aged', 'Aged, 80 and over', 'Antineoplastic Combined Chemotherapy Protocols', 'Colorectal Neoplasms', 'Female', 'Humans', 'Infusions, Intravenous', 'Leucovorin', 'Male', 'Neoplasm Metastasis', 'Organoplatinum Compounds', 'Oxaliplatin', 'Tegafur', 'Treatment Outcome', 'Uracil']
| 16,118,508
|
[['E02.319.267.100'], ['N05.715.350.075', 'N06.850.490.250'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['E02.183.750.500', 'E02.319.077.500', 'E02.319.310.037'], ['C04.588.274.476.411.307', 'C06.301.371.411.307', 'C06.405.249.411.307', 'C06.405.469.158.356', 'C06.405.469.491.307', 'C06.405.469.860.180'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.319.267.082.500', 'E02.319.267.510.590'], ['D03.633.100.733.631.400.800.350.450', 'D08.211.840.300.500'], ['C04.697.650', 'C23.550.727.650'], ['D02.691.788'], ['D02.257.750'], ['D03.383.742.698.875.404.850'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['D03.383.742.698.875']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Named Groups [M]', 'Diseases [C]', 'Organisms [B]', 'Chemicals and Drugs [D]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
The hot-film anemometer--a method for blood velocity determination. II. In vivo comparison with the electromagnetic blood flowmeter.
|
Using a constant temperature hot-film anemometer and an electromagnetic blood flowmeter, volumetric flows and velocity profiles were registered in the pulmonary artery, ascending aorta, abdominal aorta and superior vena cava of mongrel dogs. The anemometer registered in 3 out of 4 dogs in the ascending aorta and in 4 out of 5 dogs in the pulmonary artery. The flow profile in these two vessels was flat with a slight deviation with the highest velocity nearer to the posterior wall. In the abdominal aorta the flow profile was sinusoid and in the superior vena cava irregular. In 22 simultaneous measurements anemometer mean results were 97 +/- 23% (+/- SD) of flowmeter results and peak results correspondingly 113 +/- 23%. None of these differences were significant. It is stressed that both qualitatively and quantitatively hot-film anemometer results are comparable to electromagnetic flowmeter results. However, certain differences have been demonstrated.
|
['Animals', 'Aorta', 'Aorta, Abdominal', 'Blood Flow Velocity', 'Blood Pressure', 'Dogs', 'Electrocardiography', 'Electromagnetic Phenomena', 'Equipment and Supplies', 'Pulmonary Artery', 'Rheology', 'Vena Cava, Superior']
| 6,447,602
|
[]
|
[]
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
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Predictors of hazardous drinking, tobacco smoking and physical inactivity in vocational school students.
|
BACKGROUND: Tobacco smoking, hazardous drinking and physical inactivity during adolescence are risk factors that are associated with poorer health in adulthood. The identification of subgroups of young people with a high prevalence of one or more of these risk factors allows an optimised allocation of preventive measures. This study aimed at investigating hazardous drinking, tobacco smoking and physical inactivity as well as their associations and demographic predictors in vocational school students.METHODS: Out of 57 contacted vocational schools in Switzerland, a total of 24 schools participated in a survey assessing gender, age, immigrant background, educational attainment and vocational field as well as the above mentioned health risk factors. Out of the 2659 students present in 177 included vocational school classes, 2647 (99.5%) completed the survey. Binary logistic regression analyses were conducted to investigate the demographic predictors of each health risk factor and a multinomial logistic regression analysis was conducted to investigate predictors of different risk factor combinations.RESULTS: Of the surveyed students, 79.4% showed at least one risk factor, 43.6% showed two or more and 9.6% showed all three health risk factors. Hazardous drinking was more prevalent in male, physical inactivity was more prevalent in female vocational school students. The proportion of students with low physical activity and tobacco smoking increased with increasing age. While the combination of hazardous drinking and tobacco smoking was higher in males, the other risk factor combinations were observed particularly among females.CONCLUSIONS: Multiple risk factors were ascertained in a significant proportion of vocational school students. Specifically, tobacco smoking and hazardous drinking were coexistent. The study underlines the need for preventive measures in specific subpopulations of adolescents and young adults with lower educational level.
|
['Adolescent', 'Adult', 'Alcohol Drinking', 'Exercise', 'Female', 'Humans', 'Male', 'Smoking', 'Students', 'Switzerland', 'Vocational Education', 'Young Adult']
| 23,672,294
|
[['M01.060.057'], ['M01.060.116'], ['F01.145.317.269'], ['G11.427.410.698.277', 'I03.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F01.145.805'], ['M01.848'], ['Z01.542.883'], ['I02.233.862'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Organisms [B]', 'Geographicals [Z]']
| 0
| 1
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 1
|
The changing role of the nurse teacher.
|
This paper concludes a series on the findings from a study on the emerging role of nurse teachers in Project 2000 programmes. The various aspects of the study, including the literature review and methodology, have been previously discussed (Crotty & Butterworth 1992, Crotty 1992, Crotty 1993a,b,c,d). This paper highlights the main findings and suggests recommendations regarding the key issues to be addressed in the future role and preparation of the nurse teacher. The study revealed two major changes which are contrary to the expectations of Project 2000. These are firstly, the teaching of academic subjects by nurse teachers, and secondly, the rejection of clinical teaching involving 'hands on care' by nurse teachers.
|
['Delphi Technique', 'Education, Nursing', 'Faculty, Nursing', 'Humans', 'Job Description', 'Organizational Innovation', 'Teaching']
| 8,121,343
|
[['L01.906.197'], ['I02.358.462'], ['M01.526.485.390', 'M01.526.702.250.473', 'N02.360.390'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['N04.452.677.410'], ['N04.452.610'], ['I02.903']]
|
['Information Science [L]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Named Groups [M]', 'Health Care [N]', 'Organisms [B]']
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 1
| 1
| 1
| 0
|
Wire tethering or 'bowstringing' as a long-term hardware-related complication of deep brain stimulation.
|
BACKGROUND: Widely reported long-term complications following implantation of deep brain stimulation (DBS) hardware include breakage of electrode leads, internal pulse generator (IPG) failure, skin erosions and infection. Here we report on a rarely described problem that arises from formation of scar tissue adhesions around the DBS extension wire(s). Over time, this scar tissue can become tight and pronounced, protruding noticeably beneath the skin ('bowstringing' in reference to its tight bow-like appearance) and leading to significant limitation of movement and discomfort. We term this 'wire tethering'.RESULTS: We describe 6 patients with moderate to severe wire tethering. Review of our experience suggests an association of wire tethering with the passage of two extension wires on the same side as is done when using a dual-channel IPG. Five of the patients required surgical revision of the extension wires due to the magnitude of the discomfort, limitation of movement and appearance. Removing the wires was insufficient in the most severe case, necessitating transection of the scar in several places, which was done with the extension wires in situ in 2 patients. Two patients were treated with removal of the wires alone, and 1 patient did not opt for surgery and the tethering has persisted.CONCLUSION: Wire tethering, or 'bowstringing', is an underrecognized complication of DBS hardware implantation often necessitating surgical revision. The possible etiology of wire tethering is discussed as well as suggestions for its avoidance.
|
['Adult', 'Cicatrix', 'Deep Brain Stimulation', 'Dystonia', 'Electrodes, Implanted', 'Epilepsy', 'Female', 'Humans', 'Male', 'Middle Aged', 'Parkinson Disease', 'Retrospective Studies']
| 19,752,594
|
[['M01.060.116'], ['A10.165.450.300', 'C23.550.355.274', 'G16.762.891.249'], ['E02.331.300', 'E04.190'], ['C10.597.350.300', 'C23.888.592.350.300'], ['E07.305.250.319', 'E07.695.202'], ['C10.228.140.490'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['C10.228.140.079.862.500', 'C10.228.662.600.400', 'C10.574.928.750'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825']]
|
['Named Groups [M]', 'Anatomy [A]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Health Care [N]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Life Course Socioeconomic Position and Subclinical Disease: The Jackson Heart Study.
|
OBJECTIVES: African Americans experience higher rates of cardiovascular disease (CVD) and lower childhood and adult socioeconomic position (SEP). Research that examines the associations of multiple measures of SEP with subclinical CVD markers among African Americans is limited.METHODS: Data from the Jackson Heart Study (JHS) were used to examine cross-sectional associations of childhood SEP and adult SEP with subclinical markers among 4,756 African American participants (mean age 54, 64% female), adjusting for age, health behaviors and CVD risk factors. Subclinical markers included prevalent left ventricular hypertrophy (LVH), peripheral artery disease (PAD), coronary artery calcification (CAC), and carotid intima-media thickness (CIMT).RESULTS: The prevalence of LVH, PAD and CAC was 7%, 6% and 45%, respectively. The mean CIMT was .72 ± .17 mm. In fully-adjusted models, having a college education was inversely associated with PAD (OR, .27; 95% CI .13,.56) and CIMT (â=-29.7, P<.01). Income was inversely associated with LVH after adjustment for health behaviors (OR, .49 95% CI .25,.96), though associations attenuated in the fully-adjusted model. Measures of childhood SEP (material resources and mother's education) were not consistently associated with subclinical disease measures other than a positive association between material resources and CIMT.CONCLUSIONS: Subclinical disease markers were patterned by adult SEP measures among African Americans.
|
['Adult', 'African Americans', 'Aged', 'Biomarkers', 'Cardiovascular Diseases', 'Carotid Intima-Media Thickness', 'Coronary Artery Disease', 'Cross-Sectional Studies', 'Female', 'Humans', 'Hypertrophy, Left Ventricular', 'Male', 'Middle Aged', 'Prevalence', 'Risk Factors', 'Social Class']
| 27,440,975
|
[['M01.060.116'], ['M01.686.508.100.100', 'M01.686.754.100'], ['M01.060.116.100'], ['D23.101'], ['C14'], ['E01.370.350.850.150', 'E01.370.370.180', 'G09.330.210'], ['C14.280.647.250.260', 'C14.907.137.126.339', 'C14.907.585.250.260'], ['E05.318.372.500.875', 'N05.715.360.330.500.875', 'N06.850.520.450.500.875'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C14.280.195.400', 'C23.300.775.250.400'], ['M01.060.116.630'], ['E05.318.308.985.525.750', 'N01.224.935.597.750', 'N06.850.505.400.975.525.750', 'N06.850.520.308.985.525.750'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['I01.880.853.996.755', 'N01.824.782']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Organisms [B]', 'Anthropology, Education, Sociology, and Social Phenomena [I]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 0
|
A reinforcement-learning-based approach to enhance exhaustive protein loop sampling.
|
MOTIVATION: Loop portions in proteins are involved in many molecular interaction processes. They often exhibit a high degree of flexibility, which can be essential for their function. However, molecular modeling approaches usually represent loops using a single conformation. Although this conformation may correspond to a (meta-)stable state, it does not always provide a realistic representation.RESULTS: In this paper, we propose a method to exhaustively sample the conformational space of protein loops. It exploits structural information encoded in a large library of three-residue fragments, and enforces loop-closure using a closed-form inverse kinematics solver. A novel reinforcement-learning-based approach is applied to accelerate sampling while preserving diversity. The performance of our method is showcased on benchmark datasets involving 9-, 12- and 15-residue loops. In addition, more detailed results presented for streptavidin illustrate the ability of the method to exhaustively sample the conformational space of loops presenting several meta-stable conformations.AVAILABILITY AND IMPLEMENTATION: We are developing a software package called MoMA (for Molecular Motion Algorithms), which includes modeling tools and algorithms to sample conformations and transition paths of biomolecules, including the application described in this work. The binaries can be provided upon request and a web application will also be implemented in the short future.SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
|
['Algorithms', 'Models, Molecular', 'Protein Conformation', 'Proteins', 'Software']
| 31,504,192
|
[['G17.035', 'L01.224.050'], ['E05.599.595'], ['G02.111.570.820.709'], ['D12.776'], ['L01.224.900']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
The roles of phytochromes in elongation and gravitropism of roots.
|
Gravitropic orientation and the elongation of etiolated hypocotyls are both regulated by red light through the phytochrome family of photoreceptors. The importance of phytochromes A and B (phyA and phyB) in these red light responses has been established through studies using phy mutants. To identify the roles that phytochromes play in gravitropism and elongation of roots, we studied the effects of red light on root elongation and then compared the gravitropic curvature from roots of phytochrome mutants of Arabidopsis (phyA, phyB, phyD and phyAB) with wild type. We found that red light inhibits root elongation approximately 35% in etiolated seedlings and that this response is controlled by phytochromes. Roots from dark- and light-grown double mutants (phyAB) and light-grown phyB seedlings have reduced elongation rates compared with wild type. In addition, roots from these seedlings (dark/light-grown phyAB and light-grown phyB) have reduced rates of gravitropic curvature compared with wild type. These results demonstrate roles for phytochromes in regulating both the elongation and gravitropic curvature of roots.
|
['Arabidopsis', 'Arabidopsis Proteins', 'Gravitropism', 'Light', 'Mutation', 'Photoreceptor Cells', 'Phytochrome', 'Phytochrome A', 'Phytochrome B', 'Plant Roots', 'Protein-Serine-Threonine Kinases', 'Transcription Factors']
| 15,695,459
|
[['B01.650.940.800.575.912.250.157.100'], ['D12.776.765.149'], ['G07.345.249.845.400', 'G15.920.400'], ['G01.358.500.505.650', 'G01.590.540', 'G01.750.250.650', 'G01.750.770.578'], ['G05.365.590'], ['A08.675.650.850.625', 'A08.675.650.915.937', 'A08.800.950.937', 'A09.371.729.831.625', 'A11.671.650.850.625', 'A11.671.650.915.937'], ['D12.776.765.675', 'D23.767.712'], ['D08.811.913.696.620.682.700.606', 'D12.776.765.675.249', 'D23.767.712.249'], ['D12.776.765.675.500', 'D23.767.712.500'], ['A18.400'], ['D08.811.913.696.620.682.700'], ['D12.776.930']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[Neuromuscular block control during and after anaesthesia (author's transl)].
|
During many years the clinician's requirements for neuromuscular blocking drugs are satisfied by clinical investigations. Electromyographic recordings which are a satisfactory method are not useful in current practice. The use of nerve stimulators as Churchill Davidson apparatus modified for train of four impulses must reach the continental theatres, the response to neuromuscular blocking agents varying over a wide range. Five ways of stimulation can be used giving different informations, not only during anaesthesia, but so after particularly to specify the origin of some complications.
|
['Adult', 'Aged', 'Aging', 'Anesthesia', 'Child', 'Child, Preschool', 'Electromyography', 'Female', 'Humans', 'Intraoperative Period', 'Monitoring, Physiologic', 'Muscle Relaxation', 'Neuromuscular Nondepolarizing Agents', 'Postoperative Complications', 'Respiratory Insufficiency', 'Ulnar Nerve']
| 161,146
|
[['M01.060.116'], ['M01.060.116.100'], ['G07.345.124'], ['E03.155'], ['M01.060.406'], ['M01.060.406.448'], ['E01.370.405.255', 'E01.370.530.255'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E04.614.374', 'N02.421.585.753.374'], ['E01.370.520'], ['G11.427.494.554'], ['D27.505.696.663.700.710.575'], ['C23.550.767'], ['C08.618.846'], ['A08.800.800.720.050.850']]
|
['Named Groups [M]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Health Care [N]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Ajuba Phosphorylation by CDK1 Promotes Cell Proliferation and Tumorigenesis.
|
Recent studies identified the adaptor protein Ajuba as a positive regulator of Yes-associated protein (YAP) oncogenic activity through inhibiting large tumor suppressor (Lats1/2) core kinases of the Hippo pathway, a signaling pathway that plays important roles in cancer. In this study, we define a novel mechanism for phospho-regulation of Ajuba in mitosis and its biological significance in cancer. We found that Ajuba is phosphorylated in vitro and in vivo by cyclin-dependent kinase 1 (CDK1) at Ser(119) and Ser(175) during the G2/M phase of the cell cycle. Mitotic phosphorylation of Ajuba controls the expression of multiple cell cycle regulators; however, it does not affect Hippo signaling activity, nor does it induce epithelial-mesenchymal transition. We further showed that mitotic phosphorylation of Ajuba is sufficient to promote cell proliferation and anchorage-independent growth in vitro and tumorigenesis in vivo Collectively, our discoveries reveal a previously unrecognized mechanism for Ajuba regulation in mitosis and its role in tumorigenesis.
|
['Amino Acid Sequence', 'Animals', 'CDC2 Protein Kinase', 'Carcinogenesis', 'Cell Cycle', 'Cell Proliferation', 'Cyclin B', 'HeLa Cells', 'Humans', 'LIM Domain Proteins', 'Phosphorylation', 'Sequence Homology, Amino Acid']
| 27,226,586
|
[['G02.111.570.060', 'L01.453.245.667.060'], ['B01.050'], ['D08.811.913.696.620.682.700.646.500.500.250', 'D08.811.913.696.620.682.700.646.500.984.500', 'D12.644.360.250.067.249', 'D12.644.360.250.580.500', 'D12.776.167.200.067.249', 'D12.776.167.200.580.500', 'D12.776.476.250.067.249', 'D12.776.476.250.580.500', 'D12.776.744.360'], ['C04.697.098', 'C23.550.727.098'], ['G04.144'], ['G04.161.750', 'G07.345.249.410.750'], ['D12.644.360.262.120', 'D12.776.167.218.120', 'D12.776.476.262.120'], ['A11.251.210.190.400', 'A11.251.860.180.400', 'A11.436.340'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.776.512'], ['G02.111.665', 'G02.607.780', 'G03.796'], ['G02.111.810.200', 'G05.810.200']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Knowledge and practice in cardiovascular disease prevention among hospital registered nurses: a cross-sectional study.
|
AIMS AND OBJECTIVES: To investigate the knowledge and clinical practices of cardiovascular disease prevention among registered nurses who worked on three major clinical units in Beijing hospitals.BACKGROUND: Health education on cardiovascular disease prevention is an important component of nursing practice; however, Chinese registered nurses' knowledge and practice patterns have been poorly explored in previous studies.DESIGN: A cross-sectional study.METHODS: A stratified random sample of three hundred registered nurses was recruited from two tertiary hospitals in Beijing, China. A validated questionnaire was used to examine nurses' knowledge of cardiovascular disease risk factors, their practices and perceived barriers to cardiovascular disease prevention-related patient education. The differences in knowledge of cardiovascular disease risk factors and the practice pattern associated with cardiovascular disease prevention were compared among nurses who worked on three major clinical units.RESULTS: Questionnaires were completed by 273 registered nurses with a response rate of 91%. More than 75% of the registered nurses knew the cardiovascular disease risk factors; however, less than half knew the right target goals for cardiovascular disease risk factors. Notably, fewer than 70% of registered nurses routinely provided health education for cardiovascular disease prevention during their practice. There was inconsistency between registered nurses' knowledge of target goals for cardiovascular disease risk reduction and their education practices on cardiovascular disease prevention. The three major barriers to providing cardiovascular disease risk factor preventive education were lack of time, patients' reluctance to change lifestyle and lack of physicians' support.CONCLUSIONS: Not all of the registered nurses were motivated to educate and encourage patients to engage healthy lifestyle changes, even though most of them were knowledgeable about cardiovascular disease risk factors. A gap between the knowledge and practice for the prevention of cardiovascular disease was identified.RELEVANCE TO CLINICAL PRACTICE: The findings highlight the need to advocate for knowledge application and address knowledge deficits in the area of cardiovascular disease prevention among registered nurses.
|
['Adult', 'Cardiovascular Diseases', 'Cardiovascular Nursing', 'China', 'Clinical Competence', 'Cross-Sectional Studies', 'Female', 'Health Knowledge, Attitudes, Practice', 'Humans', 'Male', 'Middle Aged', "Nurse's Role", 'Nursing Staff, Hospital', 'Patient Education as Topic', 'Risk Factors', 'Surveys and Questionnaires']
| 27,906,479
|
[['M01.060.116'], ['C14'], ['H02.478.676.112', 'N02.421.533.099'], ['Z01.252.474.164'], ['I02.399.630.210', 'N04.761.210', 'N05.715.175'], ['E05.318.372.500.875', 'N05.715.360.330.500.875', 'N06.850.520.450.500.875'], ['F01.100.150.500', 'N05.300.150.410'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['F01.829.316.616.625.450', 'N05.300.100.337'], ['M01.526.485.680.490', 'M01.526.485.740.523', 'N02.360.680.490', 'N02.360.740.523'], ['I02.233.332.500', 'N02.421.726.407.680'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['E05.318.308.980', 'N05.715.360.300.800', 'N06.850.520.308.980']]
|
['Named Groups [M]', 'Diseases [C]', 'Disciplines and Occupations [H]', 'Health Care [N]', 'Geographicals [Z]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Psychiatry and Psychology [F]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 1
| 1
|
[Transconjunctival inferior blepharoplasty].
|
The transconjunctival approach is the appropriate standard for lower eyelid blepharoplasty in patients presenting with lower eyelid herniated fat without excess skin. A transconjunctival incision is made approximately 2 mm below the tarsal border and extended inferiorly following a plane posterior to the orbital septum. No conjunctival suture is necessary. Although transconjunctival blepharoplasty diminishes the occurrence of postoperative complications when compared to the transcutaneous method and constitutes a more comfortable option for the patient, we always recommend a preoperative test of the eyes. Patients must be informed of the risk of amaurosis.
|
['Adipose Tissue', 'Adult', 'Blepharoplasty', 'Blindness', 'Conjunctiva', 'Eyelids', 'Female', 'Humans', 'Middle Aged']
| 16,344,755
|
[['A10.165.114'], ['M01.060.116'], ['E04.540.104', 'E04.680.275.090'], ['C10.597.751.941.162', 'C11.966.075', 'C23.888.592.763.941.162'], ['A09.371.060.200', 'A09.371.337.168'], ['A01.456.505.420.504', 'A09.371.337'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630']]
|
['Anatomy [A]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Organisms [B]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
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