Title
stringlengths 1
395
⌀ | abstractText
stringlengths 57
5.98k
| meshMajor
stringlengths 14
1.03k
| pmid
int64 22
33.2M
| meshid
stringlengths 2
3.14k
| meshroot
stringlengths 2
421
| A
int64 0
1
| B
int64 0
1
| C
int64 0
1
| D
int64 0
1
| E
int64 0
1
| F
int64 0
1
| G
int64 0
1
| H
int64 0
1
| I
int64 0
1
| J
int64 0
1
| L
int64 0
1
| M
int64 0
1
| N
int64 0
1
| Z
int64 0
1
|
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Fragment-based screening of the bromodomain of ATAD2.
|
Cellular and genetic evidence suggest that inhibition of ATAD2 could be a useful strategy to treat several types of cancer. To discover small-molecule inhibitors of the bromodomain of ATAD2, we used a fragment-based approach. Fragment hits were identified using NMR spectroscopy, and ATAD2 was crystallized with three of the hits identified in the fragment screen.
|
['ATPases Associated with Diverse Cellular Activities', 'Adenosine Triphosphatases', 'Antineoplastic Agents', 'Binding Sites', 'Chemistry, Pharmaceutical', 'Crystallography, X-Ray', 'DNA-Binding Proteins', 'Humans', 'Kinetics', 'Ligands', 'Magnetic Resonance Spectroscopy', 'Molecular Conformation', 'Neoplasms', 'Protein Structure, Tertiary']
| 25,314,628
|
[['D08.811.277.040.013.500', 'D08.811.277.040.025.024', 'D12.776.157.025.750'], ['D08.811.277.040.025'], ['D27.505.954.248'], ['G02.111.570.120'], ['H01.158.703.007', 'H01.181.466'], ['E05.196.309.742.225'], ['D12.776.260'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G01.374.661', 'G02.111.490'], ['D27.720.470.480'], ['E05.196.867.519'], ['G02.111.570.820'], ['C04'], ['G02.111.570.820.709.610']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Disciplines and Occupations [H]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
A reappraisal of CTLA-4 checkpoint blockade in cancer immunotherapy.
|
It is assumed that anti-CTLA-4 antibodies cause tumor rejection by blocking negative signaling from B7-CTLA-4 interactions. Surprisingly, at concentrations considerably higher than plasma levels achieved by clinically effective dosing, the anti-CTLA-4 antibody Ipilimumab blocks neither B7 trans-endocytosis by CTLA-4 nor CTLA-4 binding to immobilized or cell-associated B7. Consequently, Ipilimumab does not increase B7 on dendritic cells (DCs) from either CTLA4 gene humanized (Ctla4 h/h ) or human CD34+ stem cell-reconstituted NSG™ mice. In Ctla4 h/m mice expressing both human and mouse CTLA4 genes, anti-CTLA-4 antibodies that bind to human but not mouse CTLA-4 efficiently induce Treg depletion and Fc receptor-dependent tumor rejection. The blocking antibody L3D10 is comparable to the non-blocking Ipilimumab in causing tumor rejection. Remarkably, L3D10 progenies that lose blocking activity during humanization remain fully competent in inducing Treg depletion and tumor rejection. Anti-B7 antibodies that effectively block CD4 T cell activation and de novo CD8 T cell priming in lymphoid organs do not negatively affect the immunotherapeutic effect of Ipilimumab. Thus, clinically effective anti-CTLA-4 mAb causes tumor rejection by mechanisms that are independent of checkpoint blockade but dependent on the host Fc receptor. Our data call for a reappraisal of the CTLA-4 checkpoint blockade hypothesis and provide new insights for the next generation of safe and effective anti-CTLA-4 mAbs.
|
['Animals', 'Antibodies, Blocking', 'Antineoplastic Agents, Immunological', 'CD4-Positive T-Lymphocytes', 'CD8-Positive T-Lymphocytes', 'CTLA-4 Antigen', 'Female', 'Humans', 'Immunotherapy', 'Ipilimumab', 'Lymphocyte Activation', 'Male', 'Mice', 'Mice, Inbred BALB C', 'Mice, Inbred C57BL', 'Neoplasms', 'T-Lymphocytes, Regulatory']
| 29,472,691
|
[['B01.050'], ['D12.776.124.486.485.114.143', 'D12.776.124.790.651.114.143', 'D12.776.377.715.548.114.143'], ['D27.505.954.248.384'], ['A11.118.637.555.567.569.200', 'A15.145.229.637.555.567.569.200', 'A15.382.490.555.567.569.200'], ['A11.118.637.555.567.569.220', 'A15.145.229.637.555.567.569.220', 'A15.382.490.555.567.569.220'], ['D12.776.465.782', 'D12.776.543.750.705.222.750', 'D23.050.301.264.894.158', 'D23.101.100.894.158'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.095.465.425'], ['D12.776.124.486.485.114.224.060.798', 'D12.776.124.790.651.114.224.060.798', 'D12.776.377.715.548.114.224.200.798'], ['E01.370.225.812.482', 'E05.200.812.482', 'E05.478.594.530', 'G12.450.050.400.545', 'G12.565'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.338', 'B01.050.150.900.649.313.992.635.505.500.400.338'], ['B01.050.050.199.520.520.420', 'B01.050.150.900.649.313.992.635.505.500.400.420'], ['C04'], ['A11.118.637.555.567.550.500.700', 'A11.118.637.555.567.569.200.700', 'A11.118.637.555.567.569.500.700', 'A15.145.229.637.555.567.550.500.700', 'A15.145.229.637.555.567.569.200.700', 'A15.145.229.637.555.567.569.500.700', 'A15.382.490.555.567.550.500.700', 'A15.382.490.555.567.569.200.700', 'A15.382.490.555.567.569.500.700']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Etiology of acute otitis media and phenotypic-molecular characterization of Streptococcus pneumoniae isolated from children in Liuzhou, China.
|
BACKGROUND: The etiology and epidemiology of acute otitis media (AOM) are poorly understood in China. This study aimed to describe the etiology of AOM and the phenotypic and molecular characteristics of AOM-causing Streptococcus pneumoniae (S.pneumoniae) recovered from Chinese children.METHODS: A retrospective study was conducted to enrol patients younger than 18 years diagnosed as AOM. Middle ear fluid specimens were collected then cultured for bacterial pathogens. All S.pneumoniae isolates were tested for antibiotic susceptibility, serotypes, virulence genes, antibiotic resistant determinants and sequence types.RESULTS: The dominant otopathogen among AOM children was S.pneumoniae (54.4%). Among S.pneumoniae isolates, there were 97.3, 97.3 and 75.7% isolates resistant to erythromycin, tetracycline and trimethoprim-sulfamethoxazole, respectively. There was 72.8% S.pneumoniae with multidrug resistance. The dominant sequence types (STs) were ST271 and ST320, whereas the prevailing serotypes were 19F and 19A. The 7-valent and 13-valent pneumococcal conjugate vaccine (PCV) coverage among AOM children were 73.0 and 94.6%, respectively. Additionally, we found that CC271 expressed more of mef(A/E) (P < 0.001), pspA (P = 0.022) and sipA (P < 0.001) than non-CC271 isolates.CONCLUSION: The high prevalence of international multidrug-resistant clone (Taiwan19F-14) in China necessitates continued dedication to expand PCV13 immunization and better control of antibiotic use in China.
|
['Adolescent', 'Anti-Bacterial Agents', 'Child', 'Child, Preschool', 'China', 'Drug Resistance, Bacterial', 'Female', 'Humans', 'Infant', 'Male', 'Microbial Sensitivity Tests', 'Otitis Media', 'Pneumococcal Infections', 'Pneumococcal Vaccines', 'Prevalence', 'Retrospective Studies', 'Serogroup', 'Streptococcus pneumoniae', 'Vaccines, Conjugate', 'Virulence']
| 30,770,718
|
[['M01.060.057'], ['D27.505.954.122.085'], ['M01.060.406'], ['M01.060.406.448'], ['Z01.252.474.164'], ['G06.099.225', 'G06.225.347', 'G07.690.773.984.269.347'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703'], ['E01.370.225.875.595', 'E05.200.875.595', 'E05.337.550.400'], ['C09.218.705.663'], ['C01.150.252.410.890.670'], ['D20.215.894.135.750.600'], ['E05.318.308.985.525.750', 'N01.224.935.597.750', 'N06.850.505.400.975.525.750', 'N06.850.520.308.985.525.750'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['G05.695.825'], ['B03.353.750.737.872.550', 'B03.510.400.800.872.550', 'B03.510.550.737.872.550'], ['D20.215.894.865.900', 'D23.050.865.900'], ['G06.930']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Geographicals [Z]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Health Care [N]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Proper formulation of viscous dissipation for nonlinear waves in solids.
|
To model nonlinear viscous dissipative motions in solids, acoustical physicists usually add terms linear in ?, the material time derivative of the Lagrangian strain tensor E, to the elastic stress tensor ó derived from the expansion to the third (sometimes fourth) order of the strain energy density E=E(tr E,tr E(2),tr E(3)). Here it is shown that this practice, which has been widely used in the past three decades or so, is physically wrong for at least two reasons and that it should be corrected. One reason is that the elastic stress tensor ó is not symmetric while ? is symmetric, so that motions for which ó+ó(T)?0 will give rise to elastic stresses that have no viscous pendant. Another reason is that ? is frame-invariant, while ó is not, so that an observer transformation would alter the elastic part of the total stress differently than it would alter the dissipative part, thereby violating the fundamental principle of material frame indifference. These problems can have serious consequences for nonlinear shear wave propagation in soft solids as seen here with an example of a kink in almost incompressible soft solids.
|
['Acoustics', 'Elasticity', 'Motion', 'Nonlinear Dynamics', 'Sound', 'Stress, Mechanical', 'Time Factors', 'Viscosity']
| 23,463,998
|
[['H01.671.031'], ['G01.374.590'], ['G01.482'], ['E05.599.850', 'H01.548.675'], ['G01.750.770.776'], ['G01.374.835'], ['G01.910.857'], ['G02.930']]
|
['Disciplines and Occupations [H]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 0
| 0
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
Sterilization by gamma radiation impairs the tensile fatigue life of cortical bone by two orders of magnitude.
|
Cortical bone grafts are utilized frequently for skeletal reconstruction, spinal fusion and tumor surgery. Due to its efficacy and convenience terminal sterilization by gamma radiation is often essential to minimize disease transmission and infection. However, the impairment in the material properties of bone tissue secondary to gamma radiation sterilization is a concern since the mechanical functionality of a bone graft is of primary importance. While the extent of this impairment is well investigated for monotonic loading conditions, there does not seem to exist any information on the effects of gamma radiation sterilization on cortical bone's fatigue properties, the physiologically relevant mode of loading. In this study we investigated the degradation in the high-cycle and low-cycle tensile fatigue lives of cortical bone tissue secondary to gamma radiation sterilization at a dose of 36.4 kGy which approximately falls in the higher end of the standard dose range used in tissue banking. The high-cycle and the low-cycle fatigue tests were conducted under load control at initial strain levels of 0.2% and 0.4%, respectively. Monotonic tensile tests were also conducted to compare the impairment of fatigue properties with the impairment of monotonic properties. Results demonstrated that the impairment in both the high-cycle and the low-cycle fatigue lives were two orders of magnitude following sterilization, a change much more pronounced than that observed for monotonic loading. In conclusion, the results suggest that the impairment of the mechanical function of gamma radiation sterilized allografts is even worse in fatigue than monotonically. Therefore, grafts should be designed to minimize functional strains and avoid stress raisers to prevent premature fatigue failures.
|
['Adult', 'Biomechanical Phenomena', 'Bone Transplantation', 'Bone and Bones', 'Fractures, Stress', 'Gamma Rays', 'Humans', 'Male', 'Sterilization']
| 16,140,190
|
[['M01.060.116'], ['G01.154.090', 'G01.374.089'], ['E02.095.147.725.052', 'E04.555.130', 'E04.936.580.052'], ['A02.835.232', 'A10.165.265'], ['C26.404.437'], ['G01.358.500.505.300', 'G01.750.250.300', 'G01.750.750.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['N06.850.780.200.450.850']]
|
['Named Groups [M]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Diseases [C]', 'Organisms [B]', 'Health Care [N]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Impact of New-Onset Postoperative Depression on Readmission Outcomes After Surgical Coronary Revascularization.
|
BACKGROUND: Depression affects between 10% and 40% of cardiac surgery patients and is associated with significantly worse outcomes. The incidence and impact of new-onset depression beyond acute follow-up remain ill-defined. The present study aimed to evaluate the incidence, risk factors, and prognostic implication of depression on 90-d readmission rates after coronary artery bypass grafting (CABG) surgery.METHODS: A retrospective cohort study was performed identifying adult patients without prior depression who underwent CABG surgery using the 2010-2014 National Readmissions Database. CABG patients who were readmitted more than 2 wk but within 90 d of discharge were categorized based on the presence of new-onset depression. Association between the development of new-onset depression and rehospitalization were morbidity, mortality, costs, and length of stay (LOS) and were examined using multivariable regression.RESULTS: During the study period, 1,001,945 patients underwent CABG. Of these, 11.7% of patients were readmitted after 14 d but within 90 d of discharge with 5.1% of these patients having a diagnosis of new-onset depression. Postoperative new-onset depression was not associated with increased readmission morbidity, costs, or LOS. Mortality in new-onset depression readmissions was 1.2%, compared with 2.3% in all readmitted patients (P = 0.014). Depression was associated with lower odds of mortality (OR = 0.56, P = 0.02).CONCLUSIONS: New-onset depression following CABG discharge was not associated with increased odds of mortality, morbidity, costs, or increased LOS on readmission. Rather, new-onset depression is associated with decreased odds of readmission mortality. Overall, CABG readmissions are decreasing, whereas the rate of new-onset depression is slightly increasing. Implementation of routine depression screening tools in postoperative CABG care may aid in early detection and management of depression to enhance postoperative recovery and quality of life.
|
['Aged', 'Coronary Artery Bypass', 'Coronary Artery Disease', 'Depression', 'Female', 'Follow-Up Studies', 'Hospital Mortality', 'Humans', 'Incidence', 'Male', 'Patient Readmission', 'Postoperative Complications', 'Quality of Life', 'Retrospective Studies', 'Risk Factors', 'Time Factors']
| 30,502,287
|
[['M01.060.116.100'], ['E04.100.376.719.332', 'E04.100.814.868.750', 'E04.928.220.520.220'], ['C14.280.647.250.260', 'C14.907.137.126.339', 'C14.907.585.250.260'], ['F01.145.126.350'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['E05.318.308.985.550.400', 'N01.224.935.698.400', 'N06.850.505.400.975.550.400', 'N06.850.520.308.985.550.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.308.985.525.375', 'N01.224.935.597.500', 'N06.850.505.400.975.525.375', 'N06.850.520.308.985.525.375'], ['E02.760.400.620', 'N02.421.585.400.620'], ['C23.550.767'], ['I01.800', 'K01.752.400.750', 'N06.850.505.400.425.837'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['G01.910.857']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Health Care [N]', 'Organisms [B]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Humanities [K]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 0
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 0
|
Could yeast infections impair recovery from mental illness? A case study using micronutrients and olive leaf extract for the treatment of ADHD and depression.
|
Micronutrients are increasingly used to treat psychiatric disorders including attention-deficit/hyperactivity disorder (ADHD), mood disorders, stress, and anxiety. However, a number of factors influence optimal response and absorption of nutrients, including the health of the gut, particularly the presence of yeast infections, such as Candida. As part of a wider investigation into the impact of micronutrients on psychiatric symptoms, many participants who experienced a yeast infection during their treatment showed a diminished response to the micronutrients. One case was followed systematically over a period of 3 y with documentation of deterioration in psychiatric symptoms (ADHD and mood) when infected with Candida and then symptom improvement following successful treatment of the infection with olive leaf extract (OLE) and probiotics. This case outlines that micronutrient treatment might be severely compromised by infections such as Candida and may highlight the importance of gut health when treating psychiatric disorders with nutrients. Given the role that inflammation can play in absorption of nutrients, it was hypothesized that the infection was impairing absorption of the micronutrients.
|
['Attention Deficit Disorder with Hyperactivity', 'Candidiasis, Vulvovaginal', 'Depression', 'Female', 'Humans', 'Micronutrients', 'Olea', 'Plant Extracts', 'Plant Leaves', 'Probiotics', 'Young Adult']
| 23,784,606
|
[['F03.625.094.150'], ['C01.150.703.160.190', 'C13.351.500.894.906.820.500', 'C13.351.500.944.902.737.500'], ['F01.145.126.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D27.505.696.494', 'G07.203.300.681.500', 'J02.500.681.500'], ['B01.650.940.800.575.912.250.583.640.666'], ['D20.215.784.500', 'D26.667'], ['A18.024.812'], ['G07.203.300.456.500', 'J02.500.456.500'], ['M01.060.116.815']]
|
['Psychiatry and Psychology [F]', 'Diseases [C]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Technology, Industry, and Agriculture [J]', 'Anatomy [A]', 'Named Groups [M]']
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
|
Three-dimensional visualization of nanostructured surfaces and bacterial attachment using Autodesk® Maya®.
|
There has been a growing interest in understanding the ways in which bacteria interact with nano-structured surfaces. As a result, there is a need for innovative approaches to enable researchers to visualize the biological processes taking place, despite the fact that it is not possible to directly observe these processes. We present a novel approach for the three-dimensional visualization of bacterial interactions with nano-structured surfaces using the software package Autodesk Maya. Our approach comprises a semi-automated stage, where actual surface topographic parameters, obtained using an atomic force microscope, are imported into Maya via a custom Python script, followed by a 'creative stage', where the bacterial cells and their interactions with the surfaces are visualized using available experimental data. The 'Dynamics' and 'nDynamics' capabilities of the Maya software allowed the construction and visualization of plausible interaction scenarios. This capability provides a practical aid to knowledge discovery, assists in the dissemination of research results, and provides an opportunity for an improved public understanding. We validated our approach by graphically depicting the interactions between the two bacteria being used for modeling purposes, Staphylococcus aureus and Pseudomonas aeruginosa, with different titanium substrate surfaces that are routinely used in the production of biomedical devices.
|
['Bacteria', 'Bacterial Adhesion', 'Imaging, Three-Dimensional', 'Microscopy, Atomic Force', 'Nanostructures', 'Software', 'User-Computer Interface']
| 24,577,105
|
[['B03'], ['G06.099.050'], ['E01.370.350.400', 'L01.224.308.410'], ['E01.370.350.515.666.400', 'E05.595.666.400'], ['J01.637.512'], ['L01.224.900'], ['L01.224.900.910']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Information Science [L]', 'Technology, Industry, and Agriculture [J]']
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 0
|
Axiological-Identitary Collective Action Model (AICAM): A new integrative perspective in the analysis of protest.
|
Current predictive models of collective action have devoted little attention to personal values, such as morals or ideology. The present research addresses this issue by incorporating a new axiological path in a novel predictive model of collective action, named AICAM. The axiological path is formed by two constructs: ideology and moral obligation. The model has been tested for real normative participation (Study 1) and intentional non-normative participation (Study 2). The sample for Study 1 included 531 randomly selected demonstrators and non-demonstrators at the time of a protest that took place in Madrid, May 2017. Study 2 comprised 607 randomly selected participants who filled out an online questionnaire. Structural equation modelling analysis was performed in order to examine the fit and predictive power of the model. Results show that the model is a good fit in both studies. It has also been observed that the new model entails a significant addition of overall effect size when compared with alternative models, including SIMCA. The present research contributes to the literature of collective action by unearthing a new, independent path towards collective action that is nonetheless compatible with previous motives. Implications for future research are discussed, mainly stressing the need to include moral and ideological motives in the study of collective action engagement.
|
['Civil Disorders', 'Female', 'Humans', 'Male', 'Mass Behavior', 'Models, Psychological', 'Morals', 'Motivation', 'Social Identification', 'Surveys and Questionnaires']
| 31,188,881
|
[['I01.880.735.140'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F01.145.813.527'], ['E05.599.695'], ['F01.829.500', 'K01.752.566'], ['F01.658', 'F01.752.543.500.750'], ['F01.145.813.708'], ['E05.318.308.980', 'N05.715.360.300.800', 'N06.850.520.308.980']]
|
['Anthropology, Education, Sociology, and Social Phenomena [I]', 'Organisms [B]', 'Psychiatry and Psychology [F]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Humanities [K]', 'Health Care [N]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 0
|
Occurrence and characterization of entomogenic galls in an area of Cerrado sensu stricto and Gallery forest of the state of Bahia, Brazil.
|
We surveyed insect galls in an area of Cerrado sensu stricto and Gallery forest in the municipality of Caetit? (BA) to contribute to current knowledge of the local flora and its associated gall-inducing insects. Monthly collections were made between February/2015 and January/2016, totaling 12 field campaigns (involving two or three people and lasting four hours) that followed an established path through the countryside. A total of 63 gall morphotypes were identified on 47 host plant species belonging to 22 families; 17 morphotypes were found in the Gallery forest and 46 in Cerrado vegetation. The plant families showing the greatest gall richness were Leguminosae (n=15), Myrtaceae (n=9), and Asteraceae (n=7). The species with the greatest number of galls was Mimosa gemmulata Barneby (Leguminosae) (n=3). Most galls were observed on leaves (66%) and stems (24%); they were mostly green (49.3%) or brown (26%), with globoid shapes (39.7%) or marginal roll (17.4%), and were unilocular (87%), glabrous (62%) and isolated (89%). Cecidomyiidae (Diptera) were the principal gall-inducing insects. The associated fauna was principally composed of Hymenoptera. Eight plant taxa were recorded for the first time as hosts of galling fauna.
|
['Animals', 'Brazil', 'Forests', 'Host-Parasite Interactions', 'Insecta', 'Plant Tumors', 'Plants']
| 30,304,224
|
[['B01.050'], ['Z01.107.757.176'], ['G16.500.275.157.437', 'N06.230.124.343'], ['G16.527.200.400'], ['B01.050.500.131.617'], ['G15.610.700'], ['B01.650']]
|
['Organisms [B]', 'Geographicals [Z]', 'Phenomena and Processes [G]', 'Health Care [N]']
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
|
For love and money? The impact of family structure on family income.
|
What do the half-century decline in U.S. marriage and the attendant rise in single parenthood mean for the economic well-being of children, especially children living in single-parent families? Adam Thomas and Isabel Sawhill show how differing living arrangements can be expected to affect families' economic well-being. Married-parent and cohabiting households, for example, can benefit from economies of scale and from having two adult earners. The availability of child support for single-parent families and the marriage penalties in the tax and transfer system reduce but rarely completely offset the economic benefits of marriage. Consistent with these expectations, national data on family income show that across all races and for a variety of income measures, children in lone-parent families (single-parent households with no cohabiter) have less family income and are more likely to be poor than children in married-parent families. Cohabiting families are generally better off economically than lone-parent families, but considerably worse off than married-parent families. Thomas and Sawhill acknowledge the possibility that the link between famlily structure and family resources may not be causal. But new research that simulates niarriages between existing single mothers and unattached men with similar characteristics suggests that family structure does affect family resources and that child poverty rates would drop substantially if these mothers were to marry. It does not necessarily follow, however, that policymakers ought to, or even can, do anything about family structure. Marriage is not an economic cure-all for the complex problem of child poverty. It would be a mistake for policymakers to focus on promoting marriage to the exclusion of encouraging and rewarding work or addressing problems such as early out-of-wedlock childbearing. Still, Thomas and Sawhill conclude that a continuation of recent declines in single parenthood, linked most recently to declines in teen and out-of-wedlock births, offers great promise for improving the economic welfare of U.S. children.
|
['Adult', 'Child', 'Family', 'Family Characteristics', 'Female', 'Humans', 'Income', 'Love', 'Male', 'Marriage', 'Poverty', 'United States']
| 16,158,730
|
[['M01.060.116'], ['M01.060.406'], ['F01.829.263', 'I01.880.853.150'], ['F01.829.263.315', 'I01.240.361', 'I01.880.853.150.423', 'N01.224.361', 'N01.824.308', 'N06.850.505.400.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['N01.824.417'], ['F01.470.734'], ['F01.829.263.315.500.500', 'I01.240.361.500.500', 'I01.880.853.150.423.500.500', 'N01.224.361.500.500', 'N01.824.308.500.500'], ['I01.880.735.634', 'I01.880.853.996.535', 'N01.824.600'], ['Z01.107.567.875']]
|
['Named Groups [M]', 'Psychiatry and Psychology [F]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]', 'Organisms [B]', 'Geographicals [Z]']
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 1
|
Understanding carers' fall concern and their management of fall risk among older people at home.
|
BACKGROUND: Many older people (care recipients) experience long-term psychological distress due to the fear of falling again. Falls can affect carers due to concerns about their care recipients falling. Understanding carers' fall concern is crucial to determine if carers are coping with the provision of care or have adequate knowledge and support in preventing their care recipients from falling at home.METHODS: A descriptive qualitative study was conducted to explore carers' concern about their care recipients being at risk of falling and their management of fall risk at home. Twenty-two carers were recruited from two research registers and a large tertiary hospital in a regional centre of Australia. Carers were interviewed face-to-face, or by telephone using a semi-structured interview guide about their fall concern. The data was analysed using an inductive content analysis method.RESULTS: Eight major themes emerged from the interviews. Four themes described key factors influencing carers' fall concern which include: 1) carers' perception of fall and fall risk, 2) care recipients' behaviour and attitude towards fall risk, 3) care recipients' health and function, and 4) care recipients' living environment. Another four themes described the management of care recipients' fall risk which include: 5) fall prevention strategies used, 6) risk of preventing falls, 7) support from family and friends, and 8) support from healthcare professionals.CONCLUSIONS: The findings from this qualitative study provide an insight into the carers' awareness of fall risk, knowledge, and the availability of support in preventing their care recipients from falling at home. Healthcare professionals are encouraged to include carers and address their fall concern to improve fall prevention programmes for care recipients at risk of falling at home.
|
['Accidental Falls', 'Adaptation, Psychological', 'Aged', 'Aged, 80 and over', 'Australia', 'Caregivers', 'Female', 'Humans', 'Independent Living', 'Male', 'Middle Aged', 'Qualitative Research', 'Risk Factors']
| 31,126,237
|
[['N06.850.135.122'], ['F01.058'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['Z01.639.100', 'Z01.678.100.373'], ['M01.085', 'M01.526.485.200', 'N02.360.200'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['I03.050.500', 'N01.224.791.550', 'N06.850.505.400.800.550'], ['M01.060.116.630'], ['H01.770.644.241.850'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725']]
|
['Health Care [N]', 'Psychiatry and Psychology [F]', 'Named Groups [M]', 'Geographicals [Z]', 'Organisms [B]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Disciplines and Occupations [H]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 1
| 1
|
Pseudo-rearrangement of the MLL gene at chromosome 11q23: a cautionary note on genotype analysis of leukaemia patients.
|
AIMS: The MLL gene on chromosome 11q23 is frequently disrupted by chromosomal translocations in association with haematological malignancies. Recently, a specific site within the 8.3 kb MLL break-point cluster region that is cleaved during the early stages of apoptosis has been identified. Because MLL gene rearrangements are used to identify patients with high risk leukaemia, it was the aim of this study to determine whether this DNA cleavage event could be triggered in diagnostic bone marrow samples solely through ex vivo incubation at room temperature.METHODS: Pretreatment bone marrow samples were collected from six paediatric leukaemia patients. Genomic DNA for Southern blot analysis of MLL gene rearrangements was isolated immediately after samples were obtained and compared to genomic DNA isolated after incubation of specimens for 24-60 hours at room temperature, simulating delays in processing that might occur when samples are delivered to reference laboratories. In addition, cryopreserved samples from 70 paediatric leukaemia patients were screened for evidence of site specific MLL cleavage.RESULTS: After ex vivo incubation of bone marrow samples, site specific MLL cleavage resulting in a pseudo-rearrangement of the MLL gene was detected in two of six patients. In addition, a third patient with a similar MLL pseudo-rearrangement in cryopreserved cells was identified.CONCLUSIONS: Pseudo-rearrangement of the MLL gene at chromosome 11q23 was caused by ex vivo incubation of bone marrow samples. This novel phenomenon, which could lead to misclassification of leukaemia patients, might also be of importance for genotype analysis by Southern blotting at other loci.
|
['Blotting, Southern', 'Bone Marrow', 'Child', 'Chromosomes, Human, Pair 11', 'Cryopreservation', 'Culture Techniques', 'DNA-Binding Proteins', 'Female', 'Gene Rearrangement', 'Histone-Lysine N-Methyltransferase', 'Humans', 'Infant', 'Leukemia', 'Male', 'Myeloid-Lymphoid Leukemia Protein', 'Neoplasm Proteins', 'Precursor Cell Lymphoblastic Leukemia-Lymphoma', 'Proto-Oncogenes', 'Specimen Handling', 'Transcription Factors', 'Zinc Fingers']
| 9,713,591
|
[['E05.196.401.114', 'E05.301.300.087', 'E05.601.150'], ['A15.382.216'], ['M01.060.406'], ['A11.284.187.520.300.325.355', 'G05.360.162.520.300.325.355'], ['E01.370.225.500.620.760.160', 'E01.370.225.750.600.760.160', 'E02.792.156', 'E05.200.500.620.760.160', 'E05.200.750.600.760.160', 'E05.760.156'], ['E05.481.500'], ['D12.776.260'], ['G05.344'], ['D08.811.913.555.500.800.200.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703'], ['C04.557.337'], ['D12.776.260.560', 'D12.776.624.664.700.148', 'D12.776.930.483'], ['D12.776.624'], ['C04.557.337.428.600', 'C15.604.515.560.600', 'C20.683.515.528.600'], ['G05.360.340.024.340.375.500.791'], ['E01.370.225.998', 'E05.200.998'], ['D12.776.930'], ['G02.111.570.820.709.275.500.985']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Named Groups [M]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Development of a locally sustainable functional food for people living with HIV in Sub-Saharan Africa: laboratory testing and sensory evaluation.
|
The use of Lactobacillus rhamnosus GR-1 and micronutrients has been associated with a preserved immune function among people living with HIV. However, use of these products in the developing world remains limited due to the lack of facilities for production. We describe the development of a yogurt with L. rhamnosus GR-1 at >7?10(7) colony forming units fortified with locally grown Moringa oleifera leaves at 20% of the recommended daily allowance of vitamin A. The product was made by preparing a thin paste of Moringa which was then incubated with 4% probiotic and 2% yogurt mother culture in milk for 6 hours. The addition of M. oleifera enhanced the survival of probiotic bacteria in yogurt during the shelf life period at 5 °C (P=0.02), but had no effect on probiotic survival at 21 °C. While the sensory characteristics of probiotic and non-probiotic supplemented Moringa yogurts were indistinguishable, the addition of Moringa reduced consumer acceptance compared to regular yogurt.
|
['Adolescent', 'Adult', 'Africa South of the Sahara', 'Female', 'Food Handling', 'Functional Food', 'HIV Infections', 'Humans', 'Lactobacillus rhamnosus', 'Male', 'Middle Aged', 'Moringa oleifera', 'Plant Leaves', 'Plant Preparations', 'Probiotics', 'Taste', 'Yogurt', 'Young Adult']
| 21,986,358
|
[['M01.060.057'], ['M01.060.116'], ['Z01.058.290'], ['J01.576.423.200'], ['G07.203.300.572', 'J02.500.572'], ['C01.221.250.875', 'C01.221.812.640.400', 'C01.778.640.400', 'C01.925.782.815.616.400', 'C01.925.813.400', 'C20.673.480'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['B03.353.750.450.475.700', 'B03.510.460.400.410.475.475.700', 'B03.510.550.450.475.700'], ['M01.060.116.630'], ['B01.650.940.800.575.912.250.752.500'], ['A18.024.812'], ['D20.215.784'], ['G07.203.300.456.500', 'J02.500.456.500'], ['F02.830.816.724', 'G11.561.790.724'], ['G07.203.200.500.888', 'G07.203.300.350.300.888', 'J02.350.500.888', 'J02.500.350.300.888'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Geographicals [Z]', 'Technology, Industry, and Agriculture [J]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Organisms [B]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Psychiatry and Psychology [F]']
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 1
|
[Fibromatosis of the breast].
|
Fibromatosis of the breast is a rare benign mesenchymal transformation of the connective tissue, the origin of which is probably situated in the fascia of the pectoral muscle and the Cooper's ligaments. In the clinical and radiological examination, it is difficult to differentiate between a mammary carcinoma or other malignant tumours of the breast. Only histological examination can lead to the final diagnosis. Large-scale excision of these tumours, which have a tendency to relapse, is the therapy of choice. The diagnostic problems are shown in a case of a 26-year old patient.
|
['Adult', 'Biomarkers, Tumor', 'Breast', 'Breast Neoplasms', 'Cell Division', 'Diagnosis, Differential', 'Female', 'Fibroma', 'Humans']
| 1,499,955
|
[['M01.060.116'], ['D23.101.140'], ['A01.236'], ['C04.588.180', 'C17.800.090.500'], ['G04.144.220', 'G04.161.750.500', 'G05.113', 'G07.345.249.410.750.500'], ['E01.171'], ['C04.557.450.565.590.340'], ['B01.050.150.900.649.313.988.400.112.400.400']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Different stabilities to bile among feline calicivirus strains of respiratory and enteric origin.
|
Feline calicivirus (FCV) strains isolated from feces (E-FCV) were compared with FCV strains of respiratory origin (R-FCV). All strains were shown to be labile at pH 3.0. All strains except one strain of E-FCV were found to be sensitive to the action of trypsin. When exposed to bile salt (deoxycholic acid sodium salt), all R-FCV strains were markedly inactivated, but none of the E-FCV strains was inactivated. It was possible to select bile-resistant substrains from a bile-sensitive strain.
|
['Animals', 'Antigens, Viral', 'Bile', 'Caliciviridae', 'Cat Diseases', 'Cats', 'Complement Fixation Tests', 'Deoxycholic Acid', 'Diarrhea', 'Feces', 'Neutralization Tests', 'Respiratory System', 'Respiratory Tract Infections']
| 1,626,378
|
[['B01.050'], ['D23.050.327'], ['A12.200.087'], ['B04.820.578.298'], ['C22.180'], ['B01.050.150.900.649.313.750.377.750.250.125'], ['E01.370.225.812.735.150', 'E05.200.812.735.150', 'E05.478.594.760.150'], ['D04.210.500.105.225.272', 'D04.210.500.221.430.342'], ['C23.888.821.214'], ['A12.459'], ['E01.370.225.812.735.550', 'E05.200.812.735.550', 'E05.478.594.760.550'], ['A04'], ['C01.748', 'C08.730']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Short-term repeatability of parameters extracted from radial displacement of muscle belly.
|
The aim of this study is to analyse the short-term repeatability of the parameters extracted from radial muscle belly response on a stimulation pulse. The method uses a prefixed tension of a displacement sensor tip to the muscle and is being developed for noninvasive and selective evaluation of skeletal muscle contraction properties. Five parameters were extracted and statistically evaluated from the measured displacement: maximal displacement, delay time, contraction time, sustain time and half relaxation time. Care has been taken to leave sufficient time between stimulation pulses in order to reduce the effect of muscle fatigue and a constant pre-tension was assured by controlled step motor in consecutive measurement by withdrawal and anew placement of the sensor to the muscle belly after each measurement. Intra-class correlation coefficient (ICC) and normalized squared error (NSEM) were used as measures of short-term repeatability and accuracy (precision) of the measurements while fatigue rate was evaluated using area ratio fatigue index and normalized slope. All five measured parameters have been found highly repeatable (ICC from 0.86 to 0.98) and can be measured with high precision (NSEM from 0.43 to 1.93). Maximal displacement and half relaxation time show largest influence to muscle fatigue rate and are also expected to be the best measure of the fatigue rate. This investigation should serve as an initial study of repeatability of the presented method that should help in subsequent investigations and use of the method.
|
['Adult', 'Forearm', 'Humans', 'Male', 'Muscle Contraction', 'Muscle Fatigue', 'Muscle, Skeletal', 'Reproducibility of Results']
| 17,379,538
|
[['M01.060.116'], ['A01.378.800.585'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G11.427.494'], ['G11.427.550'], ['A02.633.567', 'A10.690.552.500'], ['E05.318.370.725', 'E05.337.851', 'N05.715.360.325.685', 'N06.850.520.445.725']]
|
['Named Groups [M]', 'Anatomy [A]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Structure-activity relationship of lipopeptide Group A streptococcus (GAS) vaccine candidates on toll-like receptor 2.
|
Incorporation of lipoamino acids (LAAs) into peptide structures effectively imparts self-adjuvanting activity onto otherwise ineffective immunogens. Our fully synthetic lipopeptide vaccine candidates against group A streptococcus (GAS) were composed of J14 as a target GAS B-cell epitope alongside a universal helper T-cell epitope (P25) and a LAA-based lipid moiety. In the current study, we investigated the ability of our lipopeptides to activate nuclear factor-kappaB (NF-kappaB) in a toll-like receptor-2 (TLR2)-dependent manner as the possible mode of action and reported the structure-function requirements for novel TLR2 targeting lipopeptides based on LAAs. The NF-kappaB activation was dependent on the dose and the length of the alkyl chains of the incorporated lipid moieties with the hierarchy LAA 3 (16 carbons)>LAA 2 (14 carbons)>LAA 1 (12 carbons). The position of the lipid moiety (C-terminus vs. N(epsilon)-terminus of the central lysine residue) does not significantly affect NF-kappaB activation. Lipopeptides containing different copies of LAA 3 were synthesized and the di-lipidated analogue was the most effective in NFkappaB activation.
|
['Antigens, Bacterial', 'Cell Line', 'Epitopes, B-Lymphocyte', 'Epitopes, T-Lymphocyte', 'Genes, Reporter', 'Humans', 'Lipopeptides', 'Luciferases', 'NF-kappa B', 'Recombinant Proteins', 'Streptococcal Vaccines', 'Streptococcus pyogenes', 'Structure-Activity Relationship', 'Toll-Like Receptor 2']
| 20,045,502
|
[['D23.050.161'], ['A11.251.210'], ['D23.050.550.395'], ['D23.050.550.402'], ['G05.360.340.024.340.435'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D10.477', 'D12.644.365'], ['D08.811.682.517', 'D12.776.532.510'], ['D05.500.672', 'D12.776.260.600', 'D12.776.660.600', 'D12.776.930.600'], ['D12.776.828'], ['D20.215.894.135.750'], ['B03.353.750.737.872.575', 'B03.510.400.800.872.575', 'B03.510.550.737.872.575'], ['G02.111.830', 'G07.690.773.997'], ['D12.776.543.750.705.910.500.200']]
|
['Chemicals and Drugs [D]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Fetal alcohol exposure and temporal vulnerability: effects of binge-like alcohol exposure on the ventrolateral nucleus of the thalamus.
|
BACKGROUND: Prenatal alcohol exposure disrupts motor performance in affected offspring. The ventrolateral nucleus (VLN) of the thalamus functions to relay information between the cerebellum and motor cortex. Reductions in the size of the thalamus have been found in children with fetal alcohol syndrome, and therefore a rat model system was used to determine whether VLN size and neuronal number were altered by alcohol exposure during development.METHODS: Rat pups were exposed to alcohol in utero during the first 10 days of gestation (first trimester equivalent), the second 10 days of gestation (second trimester equivalent), or the first two trimesters equivalent combined. Some pups were exposed to alcohol in utero during the time of VLN neurogenesis. In addition, offspring from some of the dams treated during the first two trimesters equivalent were reared artificially from postnatal day (P) 4 through P9 (part of the third trimester equivalent) and received binge-like alcohol during this time, resulting in offspring exposed to alcohol during all three trimesters equivalent. Other offspring from untreated dams were reared in the same manner but received alcohol only during the third trimester equivalent. Control animals (nutritional and untreated) were reared for all treatment conditions. All pups were perfused on P10.RESULTS: A unique effect of alcohol treatment was not found for the VLN volume or the number of neural cells within the VLN. However, the period of VLN neurogenesis was found to be sensitive to both alcohol and nutritional control treatments, resulting in significant decreases in the VLN volume and neural cell number.CONCLUSIONS: Motor deficits seen in offspring exposed prenatally to alcohol do not seem to result from direct effects on the structure of the VLN of the thalamus.
|
['Animals', 'Central Nervous System Depressants', 'Ethanol', 'Female', 'Male', 'Pregnancy', 'Prenatal Exposure Delayed Effects', 'Rats', 'Rats, Sprague-Dawley', 'Ventral Thalamic Nuclei']
| 11,371,727
|
[['B01.050'], ['D27.505.696.277', 'D27.505.954.427.210'], ['D02.033.375'], ['G08.686.784.769'], ['C13.703.824.500'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.750'], ['A08.186.211.200.317.826.701.900']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Dilated intercellular space in the larynx and esophagus of a rabbit reflux model.
|
OBJECTIVE: In this study, we investigated histological and electron microscopic changes of the laryngeal and esophageal epithelium in an animal model of reflux to demonstrate: (1) the association between laryngopharyngeal reflux (LPR) and gastroesophageal reflux disease (GERD) and (2) the value of dilated intercellular space (DIS) as a marker of LPR.METHODS: Eight New Zealand albino rabbits were utilized. Four rabbits underwent total cardiomyectomy to induce reflux. The remains underwent a sham operation as controls. The animals were sacrificed 12 weeks after surgery to obtain histological and electron microscopic results.RESULTS: There were significant differences in the histological results between the study group and the control group in both the esophagus and the larynx (P=0.041 and 0.014). Significant changes in the intercellular space (IS) were observed between the study group and the control group in the esophageal and laryngeal samples (P<0.001).CONCLUSION: The results of this study suggest that LPR and GERD have a common mechanism and DIS is a morphologic marker of LPR in rabbits.
|
['Animals', 'Case-Control Studies', 'Dilatation, Pathologic', 'Disease Models, Animal', 'Esophagus', 'Extracellular Space', 'Gastroesophageal Reflux', 'Laryngeal Mucosa', 'Laryngopharyngeal Reflux', 'Larynx', 'Microscopy, Electron, Transmission', 'Mucous Membrane', 'Rabbits']
| 23,238,174
|
[['B01.050'], ['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['C23.300.325'], ['C22.232', 'E05.598.500', 'E05.599.395.080'], ['A03.556.875.500'], ['A10.082.500', 'A11.284.295'], ['C06.405.117.119.500.484'], ['A04.329.597', 'A04.760.520', 'A10.615.550.760.520'], ['C06.405.117.119.500.484.500', 'C08.360.577'], ['A04.329'], ['E01.370.350.515.402.580', 'E05.595.402.580'], ['A10.615.550'], ['B01.050.150.900.649.313.968.700']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Diseases [C]', 'Anatomy [A]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
A natural polymorphism in rDNA replication origins links origin activation with calorie restriction and lifespan.
|
Aging and longevity are complex traits influenced by genetic and environmental factors. To identify quantitative trait loci (QTLs) that control replicative lifespan, we employed an outbred Saccharomyces cerevisiae model, generated by crossing a vineyard and a laboratory strain. The predominant QTL mapped to the rDNA, with the vineyard rDNA conferring a lifespan increase of 41%. The lifespan extension was independent of Sir2 and Fob1, but depended on a polymorphism in the rDNA origin of replication from the vineyard strain that reduced origin activation relative to the laboratory origin. Strains carrying vineyard rDNA origins have increased capacity for replication initiation at weak plasmid and genomic origins, suggesting that inability to complete genome replication presents a major impediment to replicative lifespan. Calorie restriction, a conserved mediator of lifespan extension that is also independent of Sir2 and Fob1, reduces rDNA origin firing in both laboratory and vineyard rDNA. Our results are consistent with the possibility that calorie restriction, similarly to the vineyard rDNA polymorphism, modulates replicative lifespan through control of rDNA origin activation, which in turn affects genome replication dynamics.
|
['Aging', 'Caloric Restriction', 'DNA Replication', 'DNA, Ribosomal', 'DNA-Binding Proteins', 'Gene Expression Regulation, Fungal', 'Longevity', 'Polymorphism, Genetic', 'Quantitative Trait Loci', 'Replication Origin', 'Saccharomyces cerevisiae', 'Saccharomyces cerevisiae Proteins', 'Sirtuin 2']
| 23,505,383
|
[['G07.345.124'], ['E02.642.249.200', 'G07.203.650.240.340.150'], ['G02.111.225', 'G05.226'], ['D13.444.308.475'], ['D12.776.260'], ['G05.308.330'], ['G07.345.124.519', 'G07.540'], ['G05.365.795'], ['G05.360.340.024.380.937'], ['G05.360.340.024.220.760', 'G05.360.340.024.745.725'], ['B01.300.107.795.785.800', 'B01.300.930.705.655'], ['D12.776.354.750'], ['D08.811.277.087.520.200.650.200', 'D08.811.913.400.725.115.961.200', 'D12.776.476.900.200']]
|
['Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Transcatheter embolization for treatment of diverticular hemorrhage.
|
Two patients underwent transcatheter embolization for control of massive diverticular hemorrhage after vasopressin infusion had failed to control the bleeding. The role of angiography in the management of diverticular bleeding is discussed. If surgery is being considered, angiography should be utilized to localize the bleeding site and to permit limited colonic resection. Transcatheter embolization is proposed as an alternative to operative intervention.
|
['Aged', 'Catheterization', 'Diverticulum, Colon', 'Embolization, Therapeutic', 'Gastrointestinal Hemorrhage', 'Humans', 'Male', 'Mesenteric Arteries', 'Middle Aged', 'Radiography']
| 300,168
|
[['M01.060.116.100'], ['E02.148', 'E05.157'], ['C06.405.205.282.750.500', 'C23.300.415.500'], ['E02.520.360', 'E02.926.500'], ['C06.405.227', 'C23.550.414.788'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A07.015.114.565'], ['M01.060.116.630'], ['E01.370.350.700']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Organisms [B]', 'Anatomy [A]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Securing the future of drug discovery for central nervous system disorders.
|
Innovative partnerships among researchers, patients, regulators, payors and industry are needed to reinvigorate drug discovery for central nervous system disorders. Here, we summarize plans of the Collegium Internationale Neuro-Psychopharmacologicum (CINP) to achieve this goal.
|
['Animals', 'British Columbia', 'Central Nervous System Agents', 'Central Nervous System Diseases', 'Drug Discovery', 'Forecasting', 'Humans']
| 25,435,203
|
[['B01.050'], ['Z01.107.567.176.160'], ['D27.505.954.427'], ['C10.228'], ['E05.295', 'H01.158.703.007.675', 'H01.181.466.675'], ['I01.320'], ['B01.050.150.900.649.313.988.400.112.400.400']]
|
['Organisms [B]', 'Geographicals [Z]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Anthropology, Education, Sociology, and Social Phenomena [I]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 1
|
Functional characterization of the 5'-regulatory region of the human thrombomodulin gene.
|
Thrombomodulin, a glycoprotein expressed in endothelial cells, has an important role in the blood coagulation system as a modulator. Functional characterization of the 5'-regulatory region of the human thrombomodulin gene was carried out to identify elements necessary for its expression. We used a series of dissected gene constructs containing the bacterial chloramphenicol acetyltransferase gene in transient transfection assays on human umbilical vein endothelial cells. The region extending from -290 to -33 of the 5' end flanking sequence is required for the full expression of this gene. Within this region, four potential Sp1 sites were found, and the sequences of Sp1 sites were mutated to identify their role in the promoter activity of the gene, showing that the two Sp1 sites at -207 and -141 are important for the full activity of the thrombomodulin promoter. Site-directed mutation analysis identified sequence elements GCAATC at -110 as a functioning CAAT box. Another three regions, -290 to -223, -99 to -68, and -67 to -33 have unidentified positively and negatively acting elements. A silencer element was located in the region spanning from -947 to -772 bases of the 5' end flanking region. These data indicate that the expression of the thrombomodulin gene is regulated by various elements which act positively or negatively.
|
['Base Sequence', 'Cells, Cultured', 'Chloramphenicol O-Acetyltransferase', 'Endothelium, Vascular', 'Gene Expression Regulation', 'Humans', 'Molecular Sequence Data', 'Mutagenesis, Site-Directed', 'Plasmids', 'Promoter Regions, Genetic', 'Receptors, Cell Surface', 'Receptors, Thrombin', 'Regulatory Sequences, Nucleic Acid', 'Sp1 Transcription Factor', 'Transfection']
| 8,393,436
|
[['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['A11.251'], ['D08.811.913.050.134.170'], ['A07.015.700.500', 'A10.272.491.355'], ['G05.308'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['L01.453.245.667'], ['E05.393.420.601.575'], ['G05.360.600'], ['G02.111.570.080.689.675', 'G05.360.080.689.675', 'G05.360.340.024.340.137.750.680'], ['D12.776.543.750'], ['D12.776.395.550.625.800', 'D12.776.543.550.625.800', 'D12.776.543.750.695.875', 'D12.776.543.750.705.675.892', 'D12.776.543.750.750.850', 'D12.776.543.750.792.500'], ['G02.111.570.080.689', 'G05.360.080.689'], ['D12.776.260.522.750.249', 'D12.776.930.375.750.249'], ['E05.393.350.810', 'G05.728.860']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Prevalence and determinants of virological failure, genetic diversity and drug resistance among people living with HIV in a minority area in China: a population-based study.
|
BACKGROUND: Liangshan Yi Autonomous Prefecture is one of the areas that most severely affected by human immunodeficiency virus (HIV) in China, and virological failure on antiretroviral therapy (ART) is serious in this area. Analyses of prevalence and determinants of ART failure, the genetic diversity and drug resistance among people living with HIV (PLWH) helps improve HIV treatment efficiency and prevent HIV transmission.METHODS: A total of 5157 PLWH were recruited from 2016 to 2017. The venous blood samples were subjected to RT-PCR, followed by sequencing of the HIV-1 pol gene, targeting the protease and reverse transcriptase fragments. HIV-1 diversity was analyzed using the DNAStar software and drug resistance mutations were analyzed using the Stanford University HIV Drug Resistance Database.RESULTS: A total of 2156 (41.81%) PLWH showed virological failure on ART. Males (ORm = 1.25), heterosexual behaviors and drug injection (ORm = 1.44) and mother to child transmission routes (ORm = 1.58), the clinical stage of AIDS (ORm = 1.35), having used illicit drugs and shared the needles (1-4 times: ORm = 1.34; more than 5 times: ORm = 1.52), having ever replaced ART regimen (ORm = 1.48) increased the risk of virological failure among PLWH, while higher education lever (ORm = 0.77) and ? 12 months on ART (12 ~ 36 months: ORm = 0.72; ?36 months: ORm = 0.66) was associated with lower likelihood of virological failure. The data revealed that CRF07_BC (1508, 95.62%) were the most common strains, and the drug-resistant rate was 32.10% among PLWH with virological failure in this area. The high frequencies of drug resistance were found in EFV and NVP of NNRTIs, ABC, FTC and 3TC of NRTIs, and TPV/r in PIs. The most common mutations in NNRTIs, NRTIs and PIs were K103N/KN (64.69%), M184V/MV/I (36.29%) and Q58E/QE (4.93%), respectively.CONCLUSION: We concluded that surveillance of virological failure, HIV-1 subtypes, and drug resistance to understand HIV-1 epidemiology and guide modification of ART guidelines, and target prevention and control strategies should be formatted to reduce the virological failure and drug resistance to promote viral suppression and prevent HIV-1 transmission.
|
['Acquired Immunodeficiency Syndrome', 'Adolescent', 'Adult', 'Anti-HIV Agents', 'China', 'Drug Resistance, Viral', 'Female', 'Genes, pol', 'Genetic Variation', 'HIV-1', 'Humans', 'Infectious Disease Transmission, Vertical', 'Male', 'Minority Groups', 'Mutation', 'Prevalence', 'Reverse Transcriptase Inhibitors', 'Treatment Outcome', 'Young Adult']
| 32,576,136
|
[['C01.221.250.875.040', 'C01.221.812.640.400.040', 'C01.778.640.400.040', 'C01.925.782.815.616.400.040', 'C01.925.813.400.040', 'C01.925.839.040', 'C20.673.480.040'], ['M01.060.057'], ['M01.060.116'], ['D27.505.954.122.388.077.088'], ['Z01.252.474.164'], ['G06.225.420', 'G06.920.225', 'G07.690.773.984.269.420'], ['G05.360.340.024.340.364.875.667', 'G05.360.340.358.024.875.667', 'G05.360.340.358.840.500.667'], ['G05.365'], ['B04.820.650.589.650.350.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['N06.850.335.875'], ['I01.880.853.300'], ['G05.365.590'], ['E05.318.308.985.525.750', 'N01.224.935.597.750', 'N06.850.505.400.975.525.750', 'N06.850.520.308.985.525.750'], ['D27.505.519.389.675.850', 'D27.505.954.122.388.308'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['M01.060.116.815']]
|
['Diseases [C]', 'Named Groups [M]', 'Chemicals and Drugs [D]', 'Geographicals [Z]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Health Care [N]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 1
|
Profiling and analysis of multiple constituents in Baizhu Shaoyao San before and after processing by stir-frying using UHPLC/Q-TOF-MS/MS coupled with multivariate statistical analysis.
|
Baizhu Shaoyao San (BSS) is a famous traditional Chinese medicinal formula widely used for the treatment of painful diarrhea, intestinal inflammation, and diarrhea-predominant irritable bowel syndrome. According to clinical medication, three medicinal herbs (Atractylodis Macrocephalae Rhizoma, Paeoniae Radix Alba, and Citri Reticulatae Pericarpium) included in BSS must be processed using some specific methods of stir-frying. On the basis of the classical theories of traditional Chinese medicine, the therapeutic effects of BSS would be significantly enhanced after processing. Generally, the changes of curative effects mainly result from the variations of inside chemical basis caused by the processing procedure. To find out the corresponding changes of chemical compositions in BSS after processing and to elucidate the material basis of the changed curative effects, an optimized ultra-high-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry in positive and negative ion modes coupled with multivariate statistical analyses were developed. As a result, a total of 186 compounds were ultimately identified in crude and processed BSS, in which 62 marker compounds with significant differences between crude and processed BSS were found by principal component analysis and t-test. Compared with crude BSS, the contents of 23 compounds were remarkably decreased and the contents of 39 compounds showed notable increase in processed BSS. The transformation mechanisms of some changed compounds were appropriately inferred from the results. Furthermore, compounds with extremely significant differences might strengthen the effects of the whole herbal formula.
|
['Benzopyrans', 'Chromatography, High Pressure Liquid', 'Cooking', 'Drugs, Chinese Herbal', 'Flavonoids', 'Glycosides', 'Lactones', 'Multivariate Analysis', 'Tandem Mass Spectrometry', 'Terpenes']
| 29,529,536
|
[['D03.383.663.283', 'D03.633.100.150'], ['E05.196.181.400.300'], ['J01.576.423.200.200'], ['D20.215.784.500.350', 'D26.335'], ['D03.383.663.283.266.450', 'D03.633.100.150.266.450'], ['D09.408'], ['D02.540'], ['E05.318.740.150.500', 'N05.715.360.750.125.500', 'N06.850.520.830.150.500'], ['E05.196.566.880'], ['D02.455.849']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Technology, Industry, and Agriculture [J]', 'Health Care [N]']
| 0
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 0
|
Imaging for suspected appendicitis: negative appendectomy and perforation rates.
|
PURPOSE: To determine which patients suspected of having acute appendicitis benefit from preoperative imaging.MATERIALS AND METHODS: The medical records of 462 consecutive patients who underwent appendectomy for clinically suspected acute appendicitis and underwent preoperative evaluation at our institution were retrospectively reviewed. Patients were divided into four groups: women (n = 166), girls (n = 46), men (n = 178), and boys (n = 72). Preoperative computed tomography (CT) or ultrasonography (US), requested by the referring clinician, was performed in 313 of the 462 patients. Unnecessary, or negative, appendectomy and perforation rates were calculated for each group for preoperative evaluation with CT, with US, and with neither CT nor US. In addition, the sensitivity and positive predictive value of CT and US were calculated for diagnosing appendicitis.RESULTS: In women, the negative appendectomy rate was significantly lower for those who underwent preoperative CT (7% [six of 85 patients], P =.005) or US (8% [four of 49 patients], P =.019), as compared with 28% [nine of 32 patients] for those who underwent no preoperative imaging (P >.35 for all groups). The negative appendectomy rates for girls, men, and boys were not significantly affected by preoperative imaging. The sensitivity of CT and US for diagnosing acute appendicitis exceeded 93% and 77%, respectively, in all groups. The positive predictive values for both CT and US were greater than 92% in all groups.CONCLUSION: Women suspected of having appendicitis benefit the most from preoperative CT or US, with a statistically significantly lower negative appendectomy rate than women who undergo no preoperative imaging. Therefore, we propose that preoperative imaging be considered part of the routine evaluation of women suspected of having acute appendicitis.
|
['Acute Disease', 'Adolescent', 'Adult', 'Aged', 'Aged, 80 and over', 'Appendectomy', 'Appendicitis', 'Child', 'Child, Preschool', 'Contrast Media', 'False Negative Reactions', 'False Positive Reactions', 'Female', 'Humans', 'Intestinal Perforation', 'Male', 'Middle Aged', 'Predictive Value of Tests', 'Retrospective Studies', 'Rupture, Spontaneous', 'Sensitivity and Specificity', 'Tomography, X-Ray Computed', 'Ultrasonography']
| 12,354,996
|
[['C23.550.291.125'], ['M01.060.057'], ['M01.060.116'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['E04.210.078'], ['C01.463.099', 'C06.405.205.099', 'C06.405.469.110.207'], ['M01.060.406'], ['M01.060.406.448'], ['D27.505.259.500', 'D27.720.259'], ['E01.354.340'], ['E01.354.506'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C06.405.469.557'], ['M01.060.116.630'], ['E05.318.370.800.650', 'N05.715.360.325.700.640', 'N06.850.520.445.800.650'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['C23.300.909'], ['E05.318.370.800', 'E05.318.740.872', 'G17.800', 'N05.715.360.325.700', 'N05.715.360.750.725', 'N06.850.520.445.800', 'N06.850.520.830.872'], ['E01.370.350.350.810', 'E01.370.350.600.350.700.810', 'E01.370.350.700.700.810', 'E01.370.350.700.810.810', 'E01.370.350.825.810.810'], ['E01.370.350.850']]
|
['Diseases [C]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Health Care [N]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Variability in expression of Bothrops insularis snake venom proteases: an ontogenetic approach.
|
Bothrops insularis is a threatened snake endemic to Queimada Grande Island, southern coast of S?o Paulo, Brazil, and the occurrence of sexual abnormalities in males, females and intersexes (females with functional ovaries and rudimentary hemipenis) has been reported in this population. The aim of this study was to identify ontogenetic shifts in protease expression of offspring of captive-bred B. insularis. Three neonates from a single litter were maintained at the facilities of Laboratory of Herpetology, Institute Butantan, for 41 months. The snakes were individually milked and venoms were analyzed both by SDS-PAGE, under reducing conditions, and for biochemical activities. The venoms from the mother and from a pool of adult specimens were used as references. In regard to the electrophoretic patterns, common bands were identified mainly between 14 and 50 kDa among snakes. The occurrence of proteolytic activity was noticed predominantly between 27 and 45 kDa in zymograms. Inhibitory assays with 1,10-phenantroline (10 mM) and PMSF (5 mM) showed that venoms possessed both metalloproteases and serine proteases. Venoms of young specimens showed a higher coagulant activity than those of adults, especially upon factors X and II. All venoms presented fibrino(geno)lytic activity, degrading Aalpha and Bbeta chains of fibrinogen, and lysing fibrin plate. These findings can reflect important individual, ontogenetic and sexual differences on venom composition and are likely correlated with diet habits of this species.
|
['Age Factors', 'Animals', 'Bothrops', 'Crotalid Venoms', 'Factor X', 'Female', 'Male', 'Metalloproteases', 'Peptide Hydrolases']
| 17,398,162
|
[['N05.715.350.075', 'N06.850.490.250'], ['B01.050'], ['B01.050.150.900.833.672.125.937.240.375'], ['D20.888.850.960.200', 'D23.946.833.850.960.200'], ['D08.622.471', 'D12.776.124.125.400', 'D12.776.811.243.471', 'D23.119.400'], ['D08.811.277.656.675'], ['D08.811.277.656']]
|
['Health Care [N]', 'Organisms [B]', 'Chemicals and Drugs [D]']
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Long-term effects of enteral feeding on growth and mental health in adolescents with anorexia nervosa--results of a retrospective German cohort study.
|
BACKGROUND/OBJECTIVE: Anorexia nervosa (AN) is a severe eating disorder with a high mortality rate. Treatment regimes show regional and global variation and are sometimes supported by enteral feeding (EF) via nasogastric tube, although risks and benefits are still unclear. We aimed to find out whether EF improves growth and AN recovery and prevents psychiatric comorbidities.SUBJECTS/METHODS: Data were retrospectively collected from medical records and follow-up data were collected via questionnaires. Two hundred and eight female AN patients who were hospitalized below the age of 18 years with a mean follow-up of 6 years were analyzed. We calculated relative risks for the association between EF and suboptimal growth, remission of AN and the occurrence of psychiatric comorbidities, adjusting for potential confounders.RESULTS: A third of the analyzed girls received EF at any time. In the adjusted analyses, we found no significant associations between EF and suboptimal growth, the persistence of AN and the occurrence of psychiatric comorbidities, respectively.CONCLUSION: Our data suggest EF to be neither a risk factor nor beneficial for growth, recovery or persistence of AN and the occurrence of psychiatric comorbidities.
|
['Adolescent', 'Anorexia Nervosa', 'Body Mass Index', 'Cohort Studies', 'Comorbidity', 'Enteral Nutrition', 'Female', 'Germany', 'Hospitalization', 'Humans', 'Intubation, Gastrointestinal', 'Mental Disorders', 'Mental Health', 'Retrospective Studies', 'Risk Factors', 'Surveys and Questionnaires', 'Treatment Outcome', 'Weight Gain']
| 24,300,908
|
[['M01.060.057'], ['F03.400.125'], ['E01.370.600.115.100.125', 'E05.041.124.125', 'G07.100.100.125', 'N06.850.505.200.100.175'], ['E05.318.372.500.750', 'N05.715.360.330.500.750', 'N06.850.520.450.500.750'], ['N05.715.350.225', 'N06.850.490.687'], ['E02.421.360', 'E02.642.500.360'], ['Z01.542.315'], ['E02.760.400', 'N02.421.585.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.585.412', 'E05.497.412'], ['F03'], ['F02.418', 'N01.400.500'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['E05.318.308.980', 'N05.715.360.300.800', 'N06.850.520.308.980'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['C23.888.144.243.926', 'G07.345.249.314.120.200.926']]
|
['Named Groups [M]', 'Psychiatry and Psychology [F]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Geographicals [Z]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
[First-line exploration: fiberoptic gastroscopy or barium meal? A pragmatic evaluation (author's transl)].
|
The influence of the initial upper GI tract exploration upon the accuracy and cost of diagnosis was evaluated in 103 in- and out-patients of a hepato-gastroenterology unit. The patients were divided at random into two groups of comparable number, age, sex and clinical findings. One group was first examined by fiberoptic gastroscopy and the other by barium meal. In patients whose first examination was by endoscopy a second examination was less frequently needed (3/53 versus 14/50; p less than 0.01), significant lesions (e.g. oesophagitis, oesophageal varices, gastroduodenal losses of substance or tumours) were more often diagnosed (22/53 versus 11/50; p less than 0.05) and the time required for a diagnosis to be made was shorter (mean 7 +/- 6 days versus 14 +/- 16 days; p less than 0.01), even when only one examination was performed (7 +/- 6 days versus 12 +/- 15 days; p less than 0.05), than in patients first examined by barium meal. It is concluded that fiberoptic gastroscopy should be the initial method of exploration of the upper GI tract.
|
['Adult', 'Aged', 'Duodenum', 'Endoscopy', 'Esophageal Diseases', 'Esophagus', 'Female', 'Fiber Optic Technology', 'Gastrointestinal Diseases', 'Humans', 'Male', 'Middle Aged', 'Radiography', 'Stomach', 'Time Factors']
| 7,312,606
|
[['M01.060.116'], ['M01.060.116.100'], ['A03.556.124.684.124', 'A03.556.875.249'], ['E01.370.388.250', 'E04.502.250'], ['C06.405.117'], ['A03.556.875.500'], ['H01.671.617.249'], ['C06.405'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['E01.370.350.700'], ['A03.556.875.875'], ['G01.910.857']]
|
['Named Groups [M]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Disciplines and Occupations [H]', 'Organisms [B]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 1
| 0
| 0
|
Mathematical modelling of thermal process to aquatic environment with different hydrometeorological conditions.
|
This paper presents the mathematical model of the thermal process from thermal power plant to aquatic environment of the reservoir-cooler, which is located in the Pavlodar region, 17 Km to the north-east of Ekibastuz town. The thermal process in reservoir-cooler with different hydrometeorological conditions is considered, which is solved by three-dimensional Navier-Stokes equations and temperature equation for an incompressible flow in a stratified medium. A numerical method based on the projection method, divides the problem into three stages. At the first stage, it is assumed that the transfer of momentum occurs only by convection and diffusion. Intermediate velocity field is solved by fractional steps method. At the second stage, three-dimensional Poisson equation is solved by the Fourier method in combination with tridiagonal matrix method (Thomas algorithm). Finally, at the third stage, it is expected that the transfer is only due to the pressure gradient. Numerical method determines the basic laws of the hydrothermal processes that qualitatively and quantitatively are approximated depending on different hydrometeorological conditions.
|
['Computer Simulation', 'Hydrobiology', 'Models, Theoretical', 'Power Plants']
| 24,991,644
|
[['L01.224.160'], ['H01.158.273.248.750', 'H01.277.249.750'], ['E05.599'], ['J01.780', 'J03.540.680']]
|
['Information Science [L]', 'Disciplines and Occupations [H]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Technology, Industry, and Agriculture [J]']
| 0
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 0
|
Effect of vitamin D deficiency and replacement on endothelial function in asymptomatic subjects.
|
CONTEXT: Vitamin D receptors are present in many tissues. Hypovitaminosis D is considered to be a risk factor for atherosclerosis.OBJECTIVE: This study explores the effects of vitamin D replacement on insulin sensitivity, endothelial function, inflammation, oxidative stress, and leptin in vitamin D-deficient subjects.DESIGN, SETTING, AND PATIENTS: Twenty-three asymptomatic vitamin D-deficient subjects with 25-hydroxyvitamin D [25(OH)D] levels below 25 nmol/liter were compared with a control group that had a mean 25(OH)D level of 75 nmol/liter. The vitamin D-deficient group received 300,000 IU im monthly for 3 months. The following parameters were evaluated before and after treatment: vitamin D metabolites, leptin, endothelial function by brachial artery flow mediated dilatation (FMD), insulin sensitivity index based on oral glucose tolerance test, and lipid peroxidation as measures of thiobarbituric acid reactive substances (TBARS).RESULTS: FMD measurements were significantly lower in 25(OH)D-deficient subjects than controls (P = 0.001) and improved after replacement therapy (P = 0.002). Posttreatment values of TBARS were significantly lower than pretreatment levels (P < 0.001). A positive correlation between FMD and 25(OH)D (r = 0.45; P = 0.001) and a negative correlation between FMD and TBARS (r = -0.28; P < 0.05) were observed. There was a significant increase in leptin levels after therapy, and the leptin levels were positively correlated with 25(OH)D levels (r = 0.45; P < 0.05).CONCLUSIONS: This study shows that 25(OH)D deficiency is associated with endothelial dysfunction and increased lipid peroxidation. Replacement of vitamin D has favorable effects on endothelial function. Vitamin D deficiency can be seen as an independent risk factor of atherosclerosis. Hypovitaminosis D-associated endothelial dysfunction may predispose to higher rates of cardiovascular disease in the winter.
|
['Adult', 'Atherosclerosis', 'Biomarkers', 'Blood Flow Velocity', 'Blood Pressure', 'Brachial Artery', 'Endothelium, Vascular', 'Female', 'Humans', 'Insulin Resistance', 'Leptin', 'Linear Models', 'Lipid Peroxidation', 'Male', 'Oxidative Stress', 'Risk Factors', 'Treatment Outcome', 'Vitamin D', 'Vitamin D Deficiency', 'Waist-Hip Ratio']
| 19,584,181
|
[['M01.060.116'], ['C14.907.137.126.307'], ['D23.101'], ['E01.370.370.130', 'G09.330.380.630.080'], ['E01.370.600.875.249', 'G09.330.380.076'], ['A07.015.114.139'], ['A07.015.700.500', 'A10.272.491.355'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C18.452.394.968.500', 'G07.690.773.984.617'], ['D06.472.699.042.500', 'D12.644.276.024.500', 'D12.644.548.011.500', 'D12.776.467.024.500', 'D23.529.024.500'], ['E05.318.740.500.500', 'E05.318.740.750.425', 'E05.599.835.750', 'N05.715.360.750.530.460', 'N05.715.360.750.695.460', 'N06.850.520.830.500.500', 'N06.850.520.830.750.425'], ['G02.111.515', 'G03.295.531.587'], ['G03.673', 'G07.775.750'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['D04.210.500.812.768'], ['C18.654.521.500.133.770'], ['E01.370.600.115.100.960', 'E05.041.124.946', 'G07.100.100.960']]
|
['Named Groups [M]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Organisms [B]', 'Health Care [N]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Enhanced cell-surface stability of rescued DeltaF508 cystic fibrosis transmembrane conductance regulator (CFTR) by pharmacological chaperones.
|
Misfolded proteins destined for the cell surface are recognized and degraded by the ERAD [ER (endoplasmic reticulum) associated degradation] pathway. TS (temperature-sensitive) mutants at the permissive temperature escape ERAD and reach the cell surface. In this present paper, we examined a TS mutant of the CFTR [CF (cystic fibrosis) transmembrane conductance regulator], CFTR DeltaF508, and analysed its cell-surface trafficking after rescue [rDeltaF508 (rescued DeltaF508) CFTR]. We show that rDeltaF508 CFTR endocytosis is 6-fold more rapid (approximately 30% per 2.5 min) than WT (wild-type, approximately 5% per 2.5 min) CFTR at 37 degrees C in polarized airway epithelial cells (CFBE41o-). We also investigated rDeltaF508 CFTR endocytosis under two further conditions: in culture at the permissive temperature (27 degrees C) and following treatment with pharmacological chaperones. At low temperature, rDeltaF508 CFTR endocytosis slowed to WT rates (20% per 10 min), indicating that the cell-surface trafficking defect of rDeltaF508 CFTR is TS. Furthermore, rDeltaF508 CFTR is stabilized at the lower temperature; its half-life increases from <2 h at 37 degrees C to >8 h at 27 degrees C. Pharmacological chaperone treatment at 37 degrees C corrected the rDeltaF508 CFTR internalization defect, slowing endocytosis from approximately 30% per 2.5 min to approximately 5% per 2.5 min, and doubled DeltaF508 surface half-life from 2 to 4 h. These effects are DeltaF508 CFTR-specific, as pharmacological chaperones did not affect WT CFTR or transferrin receptor internalization rates. The results indicate that small molecular correctors may reproduce the effect of incubation at the permissive temperature, not only by rescuing DeltaF508 CFTR from ERAD, but also by enhancing its cell-surface stability.
|
['Animals', 'Blotting, Western', 'Cricetinae', 'Cystic Fibrosis Transmembrane Conductance Regulator', 'Endocytosis', 'Half-Life', 'HeLa Cells', 'Humans', 'Immunoprecipitation', 'Temperature']
| 18,052,931
|
[['B01.050'], ['E05.196.401.143', 'E05.301.300.096', 'E05.478.566.320.200', 'E05.601.262', 'E05.601.470.320.200'], ['B01.050.150.900.649.313.992.635.075.250'], ['D12.776.157.530.100.304.500', 'D12.776.157.530.400.175.125', 'D12.776.157.530.450.074.500.500.500.500', 'D12.776.543.550.450.175.125', 'D12.776.543.585.100.304.500', 'D12.776.543.585.400.175.125', 'D12.776.543.585.450.074.500.500.500.500'], ['G04.417'], ['G01.910.405'], ['A11.251.210.190.400', 'A11.251.860.180.400', 'A11.436.340'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.196.150.639', 'E05.478.605'], ['G01.906.595', 'G16.500.275.063.725.710', 'G16.500.750.775.710', 'N06.230.150.450', 'N06.230.300.100.725.710']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Health Care [N]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Cytological diagnostic features of late breast implant seromas: From reactive to anaplastic large cell lymphoma.
|
Late breast implant seroma may be the presentation of a breast implant-associated anaplastic large cell lymphoma (BI-ALCL), which claims for a prompt recognition. However, BI-ALCL diagnosis on fine-needle aspiration (FNA) might be challenging for pathologists lacking experience with peri-implant breast effusions. Sixty-seven late breast implant seromas collected by FNA from 50 patients were evaluated by Papanicolaou smear stain and immunocytochemistry on cell blocks. A diagnostic algorithm based on the cellular composition, cell morphology and percentage of CD30+ cells was developed. Histological evaluation of the corresponding peri-prosthetic capsules was also performed. Most of the effusions (91% of the samples) were classified as reactive and 9% as BI-ALCL. In the BI-ALCL cases, medium-to-large atypical cells expressing CD30 represented more than 70% of the cellularity, whereas in in the reactive effusions CD30+ elements were extremely rare (<5%) and consisted of non-atypical elements. The reactive effusions were categorized into three patterns: i) acute infiltrate with prominent neutrophilic component (33% of the samples); ii) mixed infiltrate characterized by a variable number of neutrophils, lymphocytes and macrophages (30% of the samples); iii) chronic infiltrate composed predominantly of T lymphocytes or macrophages with only sporadic granulocytes (37% of the samples). The inflammatory cytological patterns were consistent with the histology of the corresponding capsules. Our results indicate that cytological analysis of late breast implant effusions, supported by the knowledge of the heterogeneous cytomorphological spectrum of late seromas, is a valuable approach for the early recognition of BI-ALCL.
|
['Adult', 'Aged', 'Antigens, CD', 'Antigens, Differentiation, Myelomonocytic', 'Breast Implantation', 'Breast Neoplasms', 'CD3 Complex', 'Female', 'Gene Rearrangement', 'Humans', 'Ki-1 Antigen', 'Klebsiella oxytoca', 'Lymphocytes', 'Lymphoma, Large-Cell, Anaplastic', 'Macrophages', 'Middle Aged', 'Neutrophils', 'Pseudomonas aeruginosa', 'Receptors, Antigen, T-Cell, gamma-delta', 'Seroma', 'Serratia marcescens', 'Staphylococcus aureus', 'Young Adult']
| 28,715,445
|
[['M01.060.116'], ['M01.060.116.100'], ['D23.050.301.264.035', 'D23.101.100.110'], ['D23.050.301.264.900', 'D23.101.100.900'], ['E02.218.565.210', 'E04.650.210', 'E04.680.500.210'], ['C04.588.180', 'C17.800.090.500'], ['D23.050.301.264.894.095', 'D23.101.100.894.095'], ['G05.344'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.776.543.750.705.852.760.072', 'D23.050.285.025', 'D23.101.140.055'], ['B03.440.450.425.425.580', 'B03.660.250.150.400.580'], ['A11.118.637.555.567', 'A15.145.229.637.555.567', 'A15.382.490.555.567'], ['C04.557.386.480.750.399', 'C15.604.515.569.480.750.600', 'C20.683.515.761.480.750.399'], ['A11.329.372', 'A11.627.482', 'A11.733.397', 'A15.382.670.522', 'A15.382.680.397'], ['M01.060.116.630'], ['A11.118.637.415.583', 'A11.627.340.583', 'A11.733.689', 'A15.145.229.637.415.583', 'A15.382.490.315.583', 'A15.382.680.689'], ['B03.440.400.425.625.625.100', 'B03.660.250.580.590.050'], ['D12.776.543.750.705.816.824.830'], ['C23.550.470.640'], ['B03.440.450.425.814.664', 'B03.660.250.150.720.500'], ['B03.300.390.400.800.750.100', 'B03.353.500.750.750.100', 'B03.510.100.750.750.100', 'B03.510.400.790.750.100'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Static state hemodynamic variables estimation model for the moving-actuator type total artificial heart. Part. I--cardiac output estimation.
|
Cardiac output estimation is a very important study for the artificial heart. In this paper, we developed a cardiac output estimation model for the moving-actuator type total artificial heart (MA-TAH) that was developed at Seoul National University Hospital. The proposed model is simple and provides beat-by-beat mean cardiac output estimation. Moreover, it uses non-invasively acquired signals. Model parameters were adjusted with in vitro data by least mean square (LMS) algorithm. Results showed that the proposed scheme gives a mean estimation error of about 0.1 (l/min) for the given data. This ensures the suitability of the proposed model.
|
['Cardiac Output', 'Heart, Artificial', 'Models, Cardiovascular', 'Models, Theoretical', 'Prosthesis Design']
| 10,098,581
|
[['E01.370.370.380.150', 'G09.330.380.124'], ['E07.695.300', 'E07.858.082.374'], ['E05.599.395.161'], ['E05.599'], ['E05.320.550', 'E07.695.680']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]']
| 0
| 0
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Genetic factors influencing the risk of multiple myeloma bone disease.
|
A major complication of multiple myeloma (MM) is the development of osteolytic lesions, fractures and bone pain. To identify genetic variants influencing the development of MM bone disease (MBD), we analyzed MM patients of European ancestry (totaling 3774), which had been radiologically surveyed for MBD. Each patient had been genotyped for ~6 00 000 single-nucleotide polymorphisms with genotypes for six million common variants imputed using 1000 Genomes Project and UK10K as reference. We identified a locus at 8q24.12 for MBD (rs4407910, OPG/TNFRSF11B, odds ratio=1.38, P=4.09 ? 10(-9)) and a promising association at 19q13.43 (rs74676832, odds ratio=1.97, P=9.33 ? 10(-7)). Our findings demonstrate that germline variation influences MBD and highlights the importance of RANK/RANKL/OPG pathway in MBD development. These findings will contribute to the development of future strategies for prevention of MBD in the early precancerous phases of MM.
|
['Aged', 'Biomarkers, Tumor', 'Bone Diseases', 'Female', 'Genotype', 'High-Throughput Nucleotide Sequencing', 'Humans', 'Male', 'Middle Aged', 'Multiple Myeloma', 'Neoplasm Staging', 'Osteoprotegerin', 'Polymorphism, Single Nucleotide', 'Prognosis', 'Risk Factors']
| 26,669,972
|
[['M01.060.116.100'], ['D23.101.140'], ['C05.116'], ['G05.380'], ['E05.393.760.319'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['C04.557.595.500', 'C14.907.454.460', 'C15.378.147.780.650', 'C15.378.463.515.460', 'C20.683.515.845', 'C20.683.780.650'], ['E01.789.625'], ['D12.776.543.750.705.852.760.949.249'], ['G05.365.795.598'], ['E01.789'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Health Care [N]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Phylogeography of Ascaris lumbricoides and A. suum from China.
|
In order to obtain further understanding of genetic structure and evolutionary relationship of Ascaris from humans and pigs, phylogeography study on 12 populations from six endemic regions in China was conducted using mitochondrial DNA markers (cytochrome c oxidase subunit 1 (COX1) and NAD1) and the software programs of DnaSP 5.0, Arlequin 3.0, MEGA 4.0, and NETWORK 4.5.1.6. Results showed that (a) genetic diversity of Ascaris varied with hosts and locations, but no distinct geographical distribution pattern was found, (b) a higher level of genetic diversity and differentiation was found in pig-derived populations in contrast to human-derived ones, and in populations of human-derived Ascaris from the southern regions in comparison to that from the middle and northern locations, but similar geographical difference was not observed within pig-derived populations, (c) historical population expanding was detected from a large part of human-derived Ascaris populations but not in pig-derived Ascaris, (d) a high level of gene flow was detected between human- and pig-derived Ascaris and also among human-derived populations, and (e) network analysis from haplotype of COX1 indicated an ancestral haplotype from human-derived Ascaris. In conclusion, the present study revealed new information on Ascaris on the aspects of genetic diversity, population differentiation and historical demographic patterns, gene flow, phylogenesis reconstruction, and haplotype network, discussed the results with historical demographic migration of humans and domestication of wild boar in China, and raised a different assumption about the evolutionary relationship of the two roundworms. This study should have certain enlightenment for the epidemiology and the evolutionary and taxonomy relationship of Ascaris from humans and pigs.
|
['Animals', 'Ascariasis', 'Ascaris lumbricoides', 'Ascaris suum', 'China', 'Cluster Analysis', 'DNA, Helminth', 'Electron Transport Complex I', 'Electron Transport Complex IV', 'Female', 'Genetic Variation', 'Humans', 'Male', 'Molecular Sequence Data', 'Phylogeography', 'Sequence Analysis, DNA', 'Swine', 'Swine Diseases']
| 21,301,872
|
[['B01.050'], ['C01.610.335.508.700.100.070'], ['B01.050.500.500.294.400.500.100.108.425'], ['B01.050.500.500.294.400.500.100.108.700'], ['Z01.252.474.164'], ['E05.318.740.250', 'N05.715.360.750.200', 'N06.850.520.830.250'], ['D13.444.308.315'], ['D05.500.562.249', 'D08.811.600.250.500.500', 'D08.811.682.608.504', 'D12.776.157.427.374.375.863', 'D12.776.157.530.450.250.875.300', 'D12.776.331.199.500', 'D12.776.543.277.500.500', 'D12.776.543.585.450.250.875.437', 'D12.776.556.579.374.375.140'], ['D05.500.562.374', 'D08.811.600.250.687', 'D08.811.682.285', 'D12.776.157.530.450.250.875.304', 'D12.776.543.277.687', 'D12.776.543.585.450.250.875.484'], ['G05.365'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['L01.453.245.667'], ['H01.158.273.343.335.500', 'H01.277.500.589'], ['E05.393.760.700'], ['B01.050.150.900.649.313.500.880'], ['C22.905']]
|
['Organisms [B]', 'Diseases [C]', 'Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Disciplines and Occupations [H]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 1
|
Multiple perineuriomatous melanocytic nevi.
|
Perineuriomatous differentiation in solitary cutaneous melanocytic nevi has been described. We present an unusual case of a patient with multiple such perineuriomatous nevi. This presentation raises the possibility that a germline mutation may be responsible for the pathogenesis of these unusual lesions.
|
['Humans', 'Male', 'Middle Aged', 'Nerve Sheath Neoplasms', 'Nevus, Pigmented', 'Skin Neoplasms']
| 29,468,709
|
[['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['C04.557.580.600', 'C10.551.775.500', 'C10.668.829.725.500'], ['C04.557.665.560.615'], ['C04.588.805', 'C17.800.882']]
|
['Organisms [B]', 'Named Groups [M]', 'Diseases [C]']
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
On-line comprehensive two-dimensional separations of charged compounds using reversed-phase high performance liquid chromatography and hydrophilic interaction chromatography. Part I: orthogonality and practical peak capacity considerations.
|
Comprehensive on-line two-dimensional liquid chromatography is expected to generate impressive peak capacities, which makes it a method of choice for the analysis of complex samples such as pharmaceutical or biological ones. A comparative study of different sets of chromatographic conditions including stationary phase, mobile phase and column temperature was carried out with mixtures of representative solutes in order to find out the best two-dimensional analytical conditions for charged compounds. Our approach focused on ultra-fast gradient runs in second dimension using HT-UHPLC conditions. The choice of volatile buffers was intended for future coupling with mass spectrometry in order to get another relevant dimension. The potential of various pairs of chromatographic systems was examined by means of two-dimensional gradient data. An attempt is made to rationalize the concept of orthogonality and a method is proposed to assess, for a given pair of chromatographic systems, both the degree of orthogonality and the practical peak capacity. It is shown that the degree of orthogonality between both dimensions is a critical factor but it is not sufficient to definitely appreciate the potential of a given pair of systems. The combination of HILIC and RPLC (HILIC?RPLC or RPLC?HILIC), although providing a very high degree of orthogonality, is disappointing due to the poor peak capacities obtained in HILIC especially with peptide samples. RPLC systems offer a large variety of analytical conditions, some of them leading to appropriate degrees of orthogonality when they are combined. More importantly, due to high column efficiencies along with large separation power, some combinations of RPLC systems leads to very high practical peak capacities.
|
['Chromatography, High Pressure Liquid', 'Chromatography, Reverse-Phase', 'Hydrophobic and Hydrophilic Interactions', 'Models, Chemical', 'Peptides', 'Pharmaceutical Preparations']
| 23,022,239
|
[['E05.196.181.400.300'], ['E05.196.181.400.495'], ['G02.409'], ['E05.599.495'], ['D12.644'], ['D26']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Regulation of hypoxia-inducible factor-1alpha by NF-kappaB.
|
HIF (hypoxia-inducible factor) is the main transcription factor activated by low oxygen tensions. HIF-1alpha (and other alpha subunits) is tightly controlled mostly at the protein level, through the concerted action of a class of enzymes called PHDs (prolyl hydroxylases) 1, 2 and 3. Most of the knowledge of HIF derives from studies following hypoxic stress; however, HIF-1alpha stabilization is also found in non-hypoxic conditions through an unknown mechanism. In the present study, we demonstrate that NF-kappaB (nuclear factor kappaB) is a direct modulator of HIF-1alpha expression. The HIF-1alpha promoter is responsive to selective NF-kappaB subunits. siRNA (small interfering RNA) studies for individual NF-kappaB members revealed differential effects on HIF-1alpha mRNA levels, indicating that NF-kappaB can regulate basal HIF-1alpha expression. Finally, when endogenous NF-kappaB is induced by TNFalpha (tumour necrosis factor alpha) treatment, HIF-1alpha levels also change in an NF-kappaB-dependent manner. In conclusion, we find that NF-kappaB can regulate basal TNFalpha and, in certain circumstances, the hypoxia-induced HIF-1alpha.
|
['Cell Line, Tumor', 'Cells, Cultured', 'Humans', 'Hypoxia-Inducible Factor 1, alpha Subunit', 'NF-kappa B', 'RNA, Messenger', 'RNA, Small Interfering', 'Signal Transduction']
| 18,393,939
|
[['A11.251.210.190', 'A11.251.860.180'], ['A11.251'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.776.260.103.625.750', 'D12.776.930.125.625.750'], ['D05.500.672', 'D12.776.260.600', 'D12.776.660.600', 'D12.776.930.600'], ['D13.444.735.544'], ['D13.150.650.700', 'D13.444.735.150.700', 'D13.444.735.790.552.875'], ['G02.111.820', 'G04.835']]
|
['Anatomy [A]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Preparation of a hospital rehabilitation system for war and other disasters.
|
A large number of casualties caused by war are in need of medical rehabilitation. Rehabilitation facilities are unable to cope adequately with them without advance planning and preparedness. Guidelines are suggested for the planning and preparation of a hospital rehabilitation system for war, including the formulation of policies, expanding and strengthening of existing rehabilitation facilities, and converting institutions, as well as securing the necessary human and material resources. It is also suggested that such plans and preparations should be adapted to form a basis for meeting rehabilitation needs caused by natural and civil disasters.
|
['Disaster Planning', 'Disasters', 'Hospitals', 'Humans', 'Rehabilitation', 'United Kingdom', 'Warfare']
| 2,215,360
|
[['N06.230.100.035'], ['N06.230.100'], ['N02.278.421'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.760.169.063.500', 'E02.831', 'H02.403.680.600', 'N02.421.784'], ['Z01.542.363'], ['I01.880.735.950.500']]
|
['Health Care [N]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Geographicals [Z]', 'Anthropology, Education, Sociology, and Social Phenomena [I]']
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 1
| 1
|
A retrospective evaluation of vitamin K1 therapy to reverse the anticoagulant effect of warfarin.
|
STUDY OBJECTIVE: To assess compliance with the 2001 consensus guidelines of the American College of Chest Physicians (ACCP) regarding administration of vitamin K1 to reverse the anticoagulant effect of warfarin.DESIGN: Retrospective chart review.SETTING: University teaching hospital.PATIENTS: Fifty-five adult inpatients who received both warfarin and vitamin K1 between September 2001 and January 2002.MEASUREMENTS AND MAIN RESULTS: The patients' medical records were evaluated; data were collected on patient demographics and on vitamin K1 dosage and route of administration, warfarin dosage, and international normalized ratio (INR) before and after vitamin K1 administration. Administration routes and 87 doses of vitamin K1 prescribed for the 55 patients were assessed for compliance with the ACCP guidelines. Administration routes were subcutaneous (40.2% of doses), intravenous (35.6%), oral (13.8%), and intramuscular (10.3%). The most frequently prescribed dose of vitamin K1 was 10 mg (32.2%), followed by 2 mg (21.8%) and 5 mg (18.4%). Rates of compliance with the ACCP guidelines categorized by INR value were as follows: INR below 5, 12.2%; INR 5-9, 27.8%; INR between 9 and 20, 26.7%; and INR above 20, 0%. Four patients had documented episodes of bleeding and received seven doses of vitamin K1. Twenty-six patients received fresh frozen plasma with vitamin K1. Overall compliance with ACCP-recommended doses and routes of vitamin K1 was only 17.2%.CONCLUSION: The most frequently prescribed administration routes for vitamin K1 were subcutaneous and intravenous, indicating that the oral route is often not used as recommended. The vitamin K1 doses prescribed for reversal of warfarin anticoagulation were highly variable, and for most (83%) patients, the recommended guidelines were not followed. The clinical significance of noncompliance with the ACCP guidelines for vitamin K1 administration warrants further study.
|
['Anticoagulants', 'Antifibrinolytic Agents', 'Dose-Response Relationship, Drug', 'Drug Administration Routes', 'Drug Antagonism', 'Drug Utilization Review', 'Female', 'Hemorrhage', 'Humans', 'International Normalized Ratio', 'Male', 'Middle Aged', 'Retrospective Studies', 'Vitamin K 1', 'Warfarin']
| 14,594,342
|
[['D27.505.954.502.119'], ['D27.505.519.421.500', 'D27.505.954.502.270.463.091'], ['G07.690.773.875', 'G07.690.936.500'], ['E02.319.267'], ['G07.690.773.968.310'], ['N04.452.706.477.400', 'N04.761.879.300', 'N05.700.900.300'], ['C23.550.414'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.225.625.115.320', 'E05.200.625.115.320'], ['M01.060.116.630'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['D02.455.426.559.847.638.721.374.689', 'D02.455.849.291.523.500.689', 'D02.806.550.625', 'D04.615.638.721.374.689'], ['D03.383.663.283.446.520.914', 'D03.633.100.150.446.520.914']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Diseases [C]', 'Organisms [B]', 'Named Groups [M]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Evaluation of temporomandibular joint noise in partially edentulous patients.
|
In order to evaluate the presence of TMD (temporomandibular disorder), dissatisfaction related to the use of removable partial dentures (RPD) and the effect of the treatment on temporomandibular joint noises, 13 female patients with Kennedy class I and II mandibular arch were selected. Another 13 young, asymptomatic, dentate patients, also female, were used as reference. After four years, 38.4% were found to have a moderate or severe degree of TMD. Over the four years, the degree of TMD increased in 46.15% of the patients, was not affected in 20.07%, while in 15.38% it decreased or the patients remained free from signs and symptoms. About 30% of the patients at the second year and 46% after the fourth year, had complaints regarding retention and stability. It was concluded that there is no relationship between TMD and the condition of partially edentulous Kennedy class I and II, but patient dissatisfaction increased after the second year and temporomandibular joint noise was reduced significantly with the replacement of the teeth.
|
['Adult', 'Aged', 'Case-Control Studies', 'Dental Occlusion, Traumatic', 'Denture, Partial, Removable', 'Diagnosis, Computer-Assisted', 'Female', 'Humans', 'Jaw, Edentulous, Partially', 'Mandible', 'Middle Aged', 'Patient Satisfaction', 'Sound', 'Temporomandibular Joint Disorders', 'Vibration', 'Young Adult']
| 18,841,742
|
[['M01.060.116'], ['M01.060.116.100'], ['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['C07.793.494.293'], ['E06.780.346.760.943.413', 'E07.695.190.200.220.230'], ['E01.158', 'L01.313.500.750.100.158'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C05.500.480.450', 'C07.320.550.450', 'C07.465.550.425.450', 'C07.793.597.425.450'], ['A02.835.232.781.324.502.632', 'A14.521.632'], ['M01.060.116.630'], ['F01.100.150.750.625', 'F01.145.488.887.625', 'N04.452.822.700', 'N05.300.150.800.625', 'N05.715.360.600'], ['G01.750.770.776'], ['C05.500.607.221.897', 'C05.550.905', 'C05.651.243.897', 'C07.320.610.291.897', 'C07.678'], ['G01.374.930'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Diseases [C]', 'Information Science [L]', 'Organisms [B]', 'Anatomy [A]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 1
| 1
| 1
| 0
|
A network model to predict the risk of death in sickle cell disease.
|
Modeling the complexity of sickle cell disease pathophysiology and severity is difficult. Using data from 3380 patients accounting for all common genotypes of sickle cell disease, Bayesian network modeling of 25 clinical events and laboratory tests was used to estimate sickle cell disease severity, which was represented as a score predicting the risk of death within 5 years. The reliability of the model was supported by analysis of 2 independent patient groups. In 1 group, the severity score was related to disease severity based on the opinion of expert clinicians. In the other group, the severity score was related to the presence and severity of pulmonary hypertension and the risk of death. Along with previously known risk factors for mortality, like renal insufficiency and leukocytosis, the network identified laboratory markers of the severity of hemolytic anemia and its associated clinical events as contributing risk factors. This model can be used to compute a personalized disease severity score allowing therapeutic decisions to be made according to the prognosis. The severity score could serve as an estimate of overall disease severity in genotype-phenotype association studies, and the model provides an additional method to study the complex pathophysiology of sickle cell disease.
|
['Adolescent', 'Adult', 'Anemia, Sickle Cell', 'Blood Platelets', 'Child', 'Child, Preschool', 'Humans', 'Leukocyte Count', 'Leukocytes', 'Models, Biological', 'Platelet Count', 'Risk Factors']
| 17,600,133
|
[['M01.060.057'], ['M01.060.116'], ['C15.378.071.141.150.150', 'C15.378.420.155', 'C16.320.070.150', 'C16.320.365.155'], ['A11.118.188', 'A15.145.229.188'], ['M01.060.406'], ['M01.060.406.448'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.225.500.195.107.595', 'E01.370.225.625.107.595', 'E05.200.500.195.107.595', 'E05.200.625.107.595', 'E05.242.195.107.595', 'G04.140.107.595', 'G09.188.105.595'], ['A11.118.637', 'A15.145.229.637', 'A15.382.490'], ['E05.599.395'], ['E01.370.225.500.195.107.740', 'E01.370.225.625.107.700', 'E01.370.225.625.625.625', 'E05.200.500.195.107.740', 'E05.200.625.107.700', 'E05.200.625.625.625', 'E05.242.195.107.740', 'G04.140.107.740', 'G09.188.105.700'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725']]
|
['Named Groups [M]', 'Diseases [C]', 'Anatomy [A]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Utility of hepatic phosphorus-31 magnetic resonance spectroscopy in a rat model of acute liver failure.
|
The ability to document the extent of hepatic injury and predict the outcome of fulminant hepatic failure would be helpful in identifying those patients who might benefit from liver transplantation. The aim of the present study was to determine whether in vivo phosphorus-31 magnetic resonance spectroscopy (31P MRS) accurately assesses the severity of liver damage and is of prognostic value in a D-galactosamine (D-galN)-induced model of acute liver failure. Adult male Sprague-Dawley rats (n = 36) received an intraperitoneal dose of D-galN (1.0 g/kg), and MRS examinations were performed at peak (48 hours) and in subsequent experiments, just prior to peak (30 hours) hepatic injury. Rats not exposed to D-galN served as controls. The concentration of hepatic phosphorylated metabolites decreased in proportion to the severity of liver injury at 48 hours. Significant correlations were detected between hepatic adenosine triphosphate (ATP) and serum aspartate aminotransferase, bilirubin, and percentage of hepatocyte necrosis identified histologically (r = -.91, -.74, and -.92, respectively; p < .001). Prior to peak hepatic injury (30 hours), 31P MRS was able to predict with 100% accuracy those rats that would survive (ATP > 2.3 mM) and those that would not (ATP < 1.5 mM). When an intermediate cutoff value of 2.0 mM was selected, ATP levels were able to correctly predict survival and death with 80% and 60% accuracy, respectively. These findings indicate that hepatic ATP levels as measured by 31P MRS provide a noninvasive indication of the severity of liver damage and serve as a useful prognostic indicator of outcome in this model of acute liver failure.
|
['Animals', 'Humans', 'Liver', 'Liver Failure, Acute', 'Magnetic Resonance Spectroscopy', 'Male', 'Phosphorus', 'Prognosis', 'Rats', 'Rats, Sprague-Dawley']
| 12,580,320
|
[['B01.050'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A03.620'], ['C06.552.308.500.750'], ['E05.196.867.519'], ['D01.268.666'], ['E01.789'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.750']]
|
['Organisms [B]', 'Anatomy [A]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Optimising palliative and end of life care in hospital.
|
The acute hospital setting is increasingly regarded as an important area for the delivery of palliative care. A significant number of patients with advanced, life-limiting illness have a range of palliative care needs, some of which can be met by ward staff, but others may require additional, specialist input. Several factors have the potential to limit the palliative care patients in hospital receive, not least of these being disagreement about when and how the transition to palliative care should take place. In practice, however, palliative care can readily be delivered in conjunction with active disease management.
|
['Education, Nursing, Continuing', 'Hospitals', 'Humans', 'Palliative Care', 'Referral and Consultation', 'Terminal Care', 'United Kingdom']
| 22,848,956
|
[['I02.358.212.450', 'I02.358.462.399'], ['N02.278.421'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.760.666', 'N02.421.585.666'], ['N04.452.758.849'], ['E02.760.905', 'N02.421.585.905'], ['Z01.542.363']]
|
['Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Geographicals [Z]']
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 1
|
[Morphology of pulmonary complications in AIDS--a study of 208 biopsy and 231 autopsy cases].
|
In AIDS patients infectious pulmonary complications may very frequently be demonstrated by both biopsy and autopsy. Bacterial pneumonias occur much more frequently than classic opportunistic infections, e.g. P. carinii pneumonia. Usually, several complications are present concomitantly which impairs diagnosis as well as therapy. Increased survival and modern therapeutic modalities change the spectrum of AIDS-associated pulmonary complications as well as their morphology. In the present study pulmonary complications are directly responsible for the patients' death in more than 70% of the cases.
|
['Acquired Immunodeficiency Syndrome', 'Autopsy', 'Bacterial Infections', 'Biopsy', 'Humans', 'Lung', 'Lung Diseases', 'Lung Neoplasms', 'Lymphoma', 'Mycoses', 'Opportunistic Infections', 'Retrospective Studies', 'Sarcoma, Kaposi']
| 1,724,820
|
[['C01.221.250.875.040', 'C01.221.812.640.400.040', 'C01.778.640.400.040', 'C01.925.782.815.616.400.040', 'C01.925.813.400.040', 'C01.925.839.040', 'C20.673.480.040'], ['E01.370.060', 'E05.070', 'I01.198.780.937.120'], ['C01.150.252'], ['E01.370.225.500.384.100', 'E01.370.225.998.054', 'E01.370.388.100', 'E04.074', 'E05.200.500.384.100', 'E05.200.998.054', 'E05.242.384.100'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A04.411'], ['C08.381'], ['C04.588.894.797.520', 'C08.381.540', 'C08.785.520'], ['C04.557.386', 'C15.604.515.569', 'C20.683.515.761'], ['C01.150.703'], ['C01.597', 'C01.610.684', 'C01.925.597'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['C01.925.256.466.860', 'C04.557.450.795.850', 'C04.557.645.750']]
|
['Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Organisms [B]', 'Anatomy [A]', 'Health Care [N]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 0
|
Label-Free, Multiplexed, Single-Molecule Analysis of Protein-DNA Complexes with Nanopores.
|
Protein interactions with specific DNA sequences are crucial in the control of gene expression and the regulation of replication. Single-molecule methods offer excellent capabilities to unravel the mechanism and kinetics of these interactions. Here, we develop a nanopore approach where a target DNA sequence is contained in a hairpin followed by a ssDNA. This system allows DNA-protein complexes to be distinguished from bare DNA molecules as they are pulled through a single nanopore detector, providing both equilibrium and kinetic information. We show that this approach can be used to test the inhibitory effect of small molecules on complex formation and their mechanisms of action. In a proof of concept, we use DNAs with different sequence patterns to probe the ability of the nanopore to distinguish the effects of an inhibitor in a complex mixture of target DNAs and proteins. We anticipate that the use of this technology with arrays of thousands of nanopores will contribute to the development of transcription factor binding inhibitors.
|
['Animals', 'Biosensing Techniques', 'DNA', 'DNA, Single-Stranded', 'Drug Evaluation, Preclinical', 'Escherichia coli', 'Escherichia coli Proteins', 'Models, Molecular', 'Nanopores', 'Nanotechnology', 'Nucleic Acid Conformation', 'Protein Binding', 'Rabbits', 'Transcription Factors']
| 28,530,800
|
[['B01.050'], ['E05.601.043'], ['D13.444.308'], ['D13.444.308.497', 'G02.111.570.820.486.437', 'G05.360.580.437'], ['E05.290.750', 'E05.337.550'], ['B03.440.450.425.325.300', 'B03.660.250.150.180.100'], ['D12.776.097.275'], ['E05.599.595'], ['J01.637.512.650'], ['H01.603', 'J01.897.520.600'], ['G02.111.570.820.486', 'G05.360.580'], ['G02.111.679', 'G03.808'], ['B01.050.150.900.649.313.968.700'], ['D12.776.930']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Technology, Industry, and Agriculture [J]', 'Disciplines and Occupations [H]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
|
Organization of the cerebellum in the pigeon (Columba livia): II. Projections of the cerebellar nuclei.
|
The projections of the deep cerebellar nuclei in the pigeon have been delineated using autoradiographic and histochemical (WGA-HRP) tracing techniques. A medial (CbM) and lateral (CbL) cerebellar nucleus are recognized and CbM may be further partitioned into internal, intermediate, and intercalate divisions. As in mammals, most extracerebellar projections of CbM travel in the fasciculus uncinatus (FU); the rest travel with those of CbL in the brachium conjunctivum (BC). In the pigeon, both of these pathways are bilaterally but primarily contralaterally projecting systems. FU is a predominantly descending tract, with terminations within (1) the vestibular complex, (2) a column of contiguous medial reticular nuclei from pontine to caudal medullary levels; (3) the plexus of Horsley portion of the parvicellular reticular formation, continuing through the nucleus centralis medullae oblongatae, pars dorsalis, into intermediate layer VII of the cervical spinal cord, down to cervical segment 8-9; (4) the lateral reticular nucleus and the paragigantocellular reticular nucleus; (5) the dorsal lamella of the inferior olive. Rostrally FU terminals are found in the locus ceruleus and dorsal subcerulean nucleus. Minimal FU projections are also seen to the motor trigeminal nucleus and the subnucleus oralis of the descending trigeminal system. A small projection from the intercalate division of CbM travels in BC and projects upon the midbrain central grey, the intercollicular nucleus, the lateral tectal periventricular grey, the stratum cellulare externum and, sparsely, upon the dorsolateral thalamus. The bulk of BC originates from the lateral cerebellar nucleus and consists of a massive ascending and a small descending branch. The ascending system projects upon the red nucleus and the dorsally adjacent interstitial nucleus of Cajal and midbrain central grey, the prerubral fields continuing into the stratum cellulare externum, the nucleus intercalatus thalami, the ventrolateral thalamic nucleus, the medial spiriform nucleus, the nucleus principalis precommissuralis, the nucleus of the basal optic root, the nucleus geniculatus lateralis pars ventralis, the dorsolateral thalamus, including the dorsal intermediate posterior, and the dorsolateral intermediate and anterior nuclei. BC also contains axons from the infracerebellar nucleus, which projects upon the trochlear and the oculomotor nuclei. The descending branch of BC distributes to the papilioform nucleus, the medial pontine nucleus, the gigantocellular and paramedian reticular nuclei, and, minimally, the rostral portions of the medial column and ventral lamella of the inferior olive. Taken in conjunction with data on amphibia and reptiles the present findings suggest that the fundamental ground plan of vertebrate cerebellar organization involves a medial and lateral cerebellar nucleus.(ABSTRACT TRUNCATED AT 400 WORDS)
|
['Animals', 'Axonal Transport', 'Cerebellar Nuclei', 'Cerebellum', 'Columbidae', 'Diencephalon', 'Efferent Pathways', 'Female', 'Horseradish Peroxidase', 'Male', 'Mesencephalon', 'Pons', 'Vestibular Nuclei', 'Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate', 'Wheat Germ Agglutinins']
| 1,711,054
|
[['B01.050'], ['G03.143.355.040', 'G04.392.040', 'G11.561.050'], ['A08.186.211.132.810.428.200.337'], ['A08.186.211.132.810.428.200'], ['B01.050.150.900.248.165.150'], ['A08.186.211.200.317'], ['A08.612.380'], ['D08.811.682.732.512'], ['A08.186.211.132.659'], ['A08.186.211.132.810.428.600'], ['A08.186.211.132.810.428.600.800'], ['D08.811.682.732.512.900', 'D12.776.503.499.968.900', 'D12.776.765.678.968.900'], ['D12.776.503.499.968', 'D12.776.765.678.968']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Chemicals and Drugs [D]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Ocular reticulum cell sarcoma.
|
Three patients with ocular reticulum cell sarcoma underwent treatment with a combination of chemotherapy and irradiation. All three patients have survived an average of 36 months after treatment, significantly longer than would have been expected with radiation therapy alone. The chemotherapy appears to arrest the progression of central nervous system disease that eventually kills most patients with ocular reticulum cell sarcoma.
|
['Adult', 'Eye Neoplasms', 'Humans', 'Lymphoma, Non-Hodgkin', 'Male', 'Middle Aged']
| 7,013,487
|
[['M01.060.116'], ['C04.588.364', 'C11.319'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C04.557.386.480', 'C15.604.515.569.480', 'C20.683.515.761.480'], ['M01.060.116.630']]
|
['Named Groups [M]', 'Diseases [C]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Stroke volume and left ventricular output in preterm infants with patent ductus arteriosus.
|
To assess the effect of patent ductus arteriosus (PDA) on left ventricular output (LVO) we studied stroke volume (SV), LVO, and heart rate (HR) in 21 very low birth wt preterm neonates with clinically symptomatic PDA before and after surgical ligation. Six additional infants were also studied before PDA with left-to-right shunt was detectable by the pulsed Doppler technique. Gestational age (median and range) was 28 (24-32) wk. SV was measured by duplex Doppler and M-mode echocardiography, and LVO was calculated as product of SV and HR. LVO was 419 (305-562) mL/min/kg during symptomatic PDA. It decreased to 246 (191-292) mL/min/kg after ligation (n = 21, p less than 0.001). SV was 2.69 (1.98-4.10) mL/kg during symptomatic PDA decreasing to 1.63 (1.22-1.98) mL/kg after ductal closure (n = 21, p less than 0.001). HR did not change after ductal closure. In the six infants with three examinations, LVO and SV were normal before detectable ductal left-to-right shunt and after ligation, but LVO was increased by 59.5 +/- 23% (mean +/- SD) (p less than 0.05), and SV by 60 +/- 32% (p less than 0.05) during symptomatic PDA. In conclusion, preterm neonates with RDS, requiring mechanical ventilation, increased LVO during symptomatic PDA by increasing their SV, and not by changing their HR.
|
['Cardiac Output', 'Ductus Arteriosus, Patent', 'Echocardiography, Doppler', 'Humans', 'Infant, Newborn', 'Infant, Premature', 'Respiratory Distress Syndrome, Newborn', 'Stroke Volume']
| 2,320,395
|
[['E01.370.370.380.150', 'G09.330.380.124'], ['C14.240.400.340', 'C14.280.400.340', 'C16.131.240.400.340'], ['E01.370.350.130.750.220', 'E01.370.350.850.220.220', 'E01.370.350.850.850.220', 'E01.370.370.380.220.220'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703.520'], ['M01.060.703.520.520'], ['C08.381.840.500', 'C08.618.840.500', 'C16.614.521.563'], ['E01.370.370.380.150.700', 'G09.330.380.124.882']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Organisms [B]', 'Named Groups [M]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
[Histological transformation of low malignancy lymphoproliferative syndromes. Clinical and developmental study of 32 cases].
|
Fourteen out of 283 patients with chronic lymphoid leukaemia, 2 out of 47 with Waldenstr?m's disease and 16 out of 136 with low malignancy lymphoma (follicular with predominant small cleaved cells, mixed follicular with small and large cells, or diffuse lymphocytic) underwent histological transformation of their disease into a highly malignant lymphoma (immunoblastic in 14 of the 32 cases). There are no clinical or biological signs that predict these changes which seem to occur haphazardly in time. Seven patients died within one month of the diagnosis. The classical treatments failed. Complete remission was obtained in 5 cases with the intensive and sequential chemotherapy used for initially aggressive lymphomas.
|
['Antineoplastic Combined Chemotherapy Protocols', 'Cell Transformation, Neoplastic', 'Follow-Up Studies', 'Humans', 'Lymphatic Diseases', 'Lymphoma', 'Lymphoproliferative Disorders', 'Retrospective Studies', 'Time Factors']
| 3,160,019
|
[['E02.183.750.500', 'E02.319.077.500', 'E02.319.310.037'], ['C04.697.098.500', 'C23.550.727.098.500'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C15.604'], ['C04.557.386', 'C15.604.515.569', 'C20.683.515.761'], ['C15.604.515', 'C20.683.515'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['G01.910.857']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Health Care [N]', 'Organisms [B]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
The continuum of care protocol: a strategic response to Joint Commission change.
|
Changing mandates of the Joint Commission on Accreditation of Healthcare Organizations, including the 11 functional standards, the nine dimensions of performance, and the interdisciplinary approach, are reviewed. One hospital, City Hospital, strategically managed this change by marrying the principles of strategic management and continuous quality improvement. The end result of this pursuit was the production of a technology--a continuum grid--that helps to address accountability for hospitalwide functions, dimensions of performance, and interdisciplinary approaches while also addressing the need for a clinical pathway of care. The use of the continuum of care grid and steps to achieve a similar technology are identified.
|
['Clinical Protocols', 'Continuity of Patient Care', 'Joint Commission on Accreditation of Healthcare Organizations', 'Patient-Centered Care', 'Total Quality Management', 'United States']
| 10,154,923
|
[['E02.183', 'N05.715.360.330.125'], ['E02.760.169', 'N02.421.585.169', 'N04.590.233.727.210'], ['N03.706.110.070.410', 'N05.700.200.100.420'], ['N04.590.233.727.407'], ['N04.452.955', 'N04.761.700.675', 'N05.700.792'], ['Z01.107.567.875']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Geographicals [Z]']
| 0
| 0
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
|
Discrimination of natural scenes in central and peripheral vision.
|
We conducted suprathreshold discrimination experiments to compare how natural-scene information is processed in central and peripheral vision (16° eccentricity). Observers' ratings of the perceived magnitude of changes in naturalistic scenes were lower for peripheral than for foveal viewing, and peripheral orientation changes were rated less than peripheral colour changes. A V1-based Visual Difference Predictor model of the magnitudes of perceived foveal change was adapted to match the sinusoidal grating sensitivities of peripheral vision, but it could not explain why the ratings for changes in peripheral stimuli were so reduced. Perceived magnitude ratings for peripheral stimuli were further reduced by simultaneous presentation of flanking patches of naturalistic images, a phenomenon that could not be replicated foveally, even after M-scaling the foveal stimuli to reduce their size and the distances from the flankers. The effects of the peripheral flankers are very reminiscent of crowding phenomena demonstrated with letters or Gabor patches.
|
['Color Perception', 'Contrast Sensitivity', 'Discrimination, Psychological', 'Fixation, Ocular', 'Humans', 'Orientation', 'Sensory Thresholds', 'Space Perception', 'Vision, Ocular', 'Visual Fields', 'Visual Perception']
| 21,640,747
|
[['F02.463.593.932.217'], ['E01.370.380.850.950.500', 'F02.463.593.778.435.110', 'F02.463.593.932.281', 'F02.463.593.932.901.500', 'G14.940.500'], ['F02.463.593.257'], ['G14.350.253'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F01.058.577', 'F02.830.606'], ['F02.463.593.710'], ['F02.463.593.778'], ['F02.830.816.964', 'G02.111.820.480.900', 'G04.835.480.900', 'G11.561.790.964', 'G14.935'], ['F02.463.593.932.934', 'G14.950'], ['F02.463.593.932']]
|
['Psychiatry and Psychology [F]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 0
| 1
| 0
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Differential modulation of endotoxin responsiveness by human caspase-12 polymorphisms.
|
Caspases mediate essential key proteolytic events in inflammatory cascades and the apoptotic cell death pathway. Human caspases functionally segregate into two distinct subfamilies: those involved in cytokine maturation (caspase-1, -4 and -5) and those involved in cellular apoptosis (caspase-2, -3, -6, -7, -8, -9 and -10). Although caspase-12 is phylogenetically related to the cytokine maturation caspases, in mice it has been proposed as a mediator of apoptosis induced by endoplasmic reticulum stress including amyloid-beta cytotoxicity, suggesting that it might contribute to the pathogenesis of Alzheimer's disease. Here we show that a single nucleotide polymorphism in caspase-12 in humans results in the synthesis of either a truncated protein (Csp12-S) or a full-length caspase proenzyme (Csp12-L). The read-through single nucleotide polymorphism encoding Csp12-L is confined to populations of African descent and confers hypo-responsiveness to lipopolysaccharide-stimulated cytokine production in ex vivo whole blood, but has no significant effect on apoptotic sensitivity. In a preliminary study, we find that the frequency of the Csp12-L allele is increased in African American individuals with severe sepsis. Thus, Csp12-L attenuates the inflammatory and innate immune response to endotoxins and in doing so may constitute a risk factor for developing sepsis.
|
['Africa', 'African Americans', 'Alzheimer Disease', 'Animals', 'Apoptosis', 'Base Sequence', 'Case-Control Studies', 'Caspase 12', 'Caspases', 'Concanavalin A', 'Cytokines', 'Endoplasmic Reticulum', 'Gene Frequency', 'Genetic Predisposition to Disease', 'Genotype', 'Humans', 'Inflammation', 'Lipopolysaccharides', 'NF-kappa B', 'Polymorphism, Single Nucleotide', 'Primates', 'Sepsis']
| 15,129,283
|
[['Z01.058'], ['M01.686.508.100.100', 'M01.686.754.100'], ['C10.228.140.380.100', 'C10.574.945.249', 'F03.615.400.100'], ['B01.050'], ['G04.146.954.035'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['D08.811.277.656.262.500.126.550.920', 'D08.811.277.656.300.200.126.550.920', 'D12.644.360.075.405.550.920', 'D12.776.476.075.405.550.920'], ['D08.811.277.656.262.500.126', 'D08.811.277.656.300.200.126', 'D12.644.360.075.405', 'D12.776.476.075.405'], ['D12.776.503.499.500', 'D12.776.765.678.500'], ['D12.644.276.374', 'D12.776.467.374', 'D23.529.374'], ['A11.284.430.214.190.875.248'], ['G05.330'], ['C23.550.291.687.500', 'G05.380.355'], ['G05.380'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C23.550.470'], ['D09.400.500', 'D09.698.718.450', 'D10.494', 'D23.050.161.616.525', 'D23.946.123.329.500'], ['D05.500.672', 'D12.776.260.600', 'D12.776.660.600', 'D12.776.930.600'], ['G05.365.795.598'], ['B01.050.150.900.649.313.988'], ['C01.757', 'C23.550.470.790.500']]
|
['Geographicals [Z]', 'Named Groups [M]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Chemicals and Drugs [D]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 1
| 1
| 1
| 1
|
Nonsustained reentry following successive stimulation of cardiac tissue through a unipolar electrode.
|
INTRODUCTION: Using numerical simulations, we predict that nonsustained reentry occurs following a strong, premature stimulus through a unipolar electrode.METHODS AND RESULTS: Our simulations were based on the bidomain model of cardiac tissue, and the active membrane properties were represented by the Beeler-Reuter model. An outwardly propagating wavefront was excited by an initial stimulus (S1). A second stimulus (S2) was then applied through the same electrode. Nonsustained reentry or reentrant-like behavior followed the S2 stimulus for both cathodal and anodal stimulation, and were associated with "break" stimulation but not with "make" stimulation. The direction of spiral-wave rotation was reversed when the polarity of the stimulus was reversed. These complex dynamics occur only for a narrow window of S1-S2 intervals. During anodal S2 stimulation, two different modes of reentry exist. Our simulations also explain the "no response" phenomenon.CONCLUSION: Our mathematical model predicts that both anodal and cathodal unipolar S2 stimulation results in reentry. This behavior arises from an interaction of virtual anodes and cathodes surrounding the stimulating electrode.
|
['Computer Simulation', 'Electric Conductivity', 'Electric Stimulation', 'Electrodes', 'Evoked Potentials', 'Heart', 'Humans', 'Membrane Potentials', 'Models, Cardiovascular']
| 9,255,684
|
[['L01.224.160'], ['G01.358.500.249.277'], ['E05.723.402'], ['E07.305.250'], ['G07.265.216.500', 'G11.561.200.500'], ['A07.541'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G01.154.535', 'G04.580', 'G07.265.675', 'G11.561.570'], ['E05.599.395.161']]
|
['Information Science [L]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Organisms [B]']
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Evidence for the asymmetrical binding of p-chloromercuriphenyl sulphonate to the human erythrocyte nucleoside transporter.
|
Nucleosides cross the human erythrocyte membrane by a facilitated-diffusion process which is selectively inhibited by nanomolar concentrations of nitrobenzylthioinosine (NBMPR). The chemical asymmetry of the transporter was investigated by studying the effects of p-chloromercuriphenyl sulphonate (PCMBS) on uridine transport and high-affinity NBMPR binding in inside-out and right-side-out membrane vesicles, unsealed erythrocyte ghosts and intact cells. PCMBS was an effective inhibitor of the transporter (50% inhibition at 30 microM), but only when the organomercurial had access to the cytoplasmic membrane surface. PCMBS inhibition of NBMPR binding to ghosts was reversed by incubation with dithiothreitol. Both uridine and NBMPR were able to protect the transporter against PCMBS inhibition.
|
['4-Chloromercuribenzenesulfonate', 'Biological Transport, Active', 'Blood Proteins', 'Dithiothreitol', 'Erythrocyte Membrane', 'Guanosine', 'Humans', 'Membrane Proteins', 'Nucleoside Transport Proteins', 'Phenylmercury Compounds', 'Structure-Activity Relationship', 'Thioinosine', 'Thionucleosides', 'Uridine']
| 2,994,728
|
[['D02.455.426.559.389.097.150', 'D02.691.750.740.220', 'D02.886.645.600.080.050.100.100'], ['G03.143.310'], ['D12.776.124'], ['D02.033.800.196', 'D02.886.740.224', 'D09.853.196'], ['A11.118.290.270', 'A11.284.149.356', 'A15.145.229.334.270'], ['D03.633.100.759.590.454', 'D13.570.583.454', 'D13.570.800.453'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.776.543'], ['D12.776.157.530.625.750', 'D12.776.543.585.625.750'], ['D02.691.750.740'], ['G02.111.830', 'G07.690.773.997'], ['D02.886.759.800', 'D03.633.100.759.590.616.900', 'D13.570.583.616.900', 'D13.570.800.573.900', 'D13.570.900.800'], ['D02.886.759', 'D13.570.900'], ['D03.383.742.680.852', 'D13.570.685.852', 'D13.570.800.892']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Organisms [B]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
The Category Cued Recall test in very mild Alzheimer's disease: discriminative validity and correlation with semantic memory functions.
|
Episodic memory tests that measure cued recall may be particularly effective in the diagnosis of early Alzheimer's disease (AD) because they examine both episodic and semantic memory functions. The Category Cued Recall (CCR) test provides superordinate semantic cues at encoding and retrieval, and high discriminative validity has been claimed for this test. The aim of this study was to investigate the discriminative validity for this test when compared with the 10-word memory list from Alzheimer's Disease Assessment Scale (ADAS-cog) that measures free recall. The clinical diagnosis of AD was taken as the standard. It was also investigated whether the two episodic memory tests correlated with measures of semantic memory. The tests were administered to 35 patients with very mild AD (Mini Mental State Examination score >22) and 28 control subjects. Both tests had high sensitivity (>88%) with high specificity (>89%). One out of the five semantic memory tests was significantly correlated to performances on CCR, whereas delayed recall on the ADAS-cog memory test was significantly correlated to two semantic tests. In conclusion, the discriminative validity of the CCR test and the ADAS-cog memory test was equivalent in very mild AD. This may be because CCR did not tap more semantic processes, which are impaired in the earliest phases of AD, than a test of free recall.
|
['Aged', 'Alzheimer Disease', 'Cues', 'Discrimination, Psychological', 'Female', 'Humans', 'Male', 'Memory', 'Mental Recall', 'Neuropsychological Tests', 'Predictive Value of Tests', 'Semantics']
| 17,222,122
|
[['M01.060.116.100'], ['C10.228.140.380.100', 'C10.574.945.249', 'F03.615.400.100'], ['F02.463.425.234'], ['F02.463.593.257'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F02.463.425.540'], ['F02.463.425.540.641'], ['F04.711.513'], ['E05.318.370.800.650', 'N05.715.360.325.700.640', 'N06.850.520.445.800.650'], ['L01.559.598.745']]
|
['Named Groups [M]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Information Science [L]']
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
| 0
|
Safety of Simultaneous Coronary Artery Bypass Grafting and Carotid Endarterectomy Versus Isolated Coronary Artery Bypass Grafting: A Randomized Clinical Trial.
|
BACKGROUND AND PURPOSE: The optimal operative strategy in patients with severe carotid artery disease undergoing coronary artery bypass grafting (CABG) is unknown. We sought to investigate the safety and efficacy of synchronous combined carotid endarterectomy and CABG as compared with isolated CABG.METHODS: Patients with asymptomatic high-grade carotid artery stenosis ?80% according to ECST (European Carotid Surgery Trial) ultrasound criteria (corresponding to ?70% NASCET [North American Symptomatic Carotid Endarterectomy Trial]) who required CABG surgery were randomly assigned to synchronous carotid endarterectomy+CABG or isolated CABG. To avoid unbalanced prognostic factor distributions, randomization was stratified by center, age, sex, and modified Rankin Scale. The primary composite end point was the rate of stroke or death at 30 days.RESULTS: From 2010 to 2014, a total of 129 patients were enrolled at 17 centers in Germany and the Czech Republic. Because of withdrawal of funding after insufficient recruitment, enrolment was terminated early. At 30 days, the rate of any stroke or death in the intention-to-treat population was 12/65 (18.5%) in patients receiving synchronous carotid endarterectomy+CABG as compared with 6/62 (9.7%) in patients receiving isolated CABG (absolute risk reduction, 8.8%; 95% confidence interval, -3.2% to 20.8%; PWALD=0.12). Also for all secondary end points at 30 days and 1 year, there was no evidence for a significant treatment-group effect although patients undergoing isolated CABG tended to have better outcomes.CONCLUSIONS: Although our results cannot rule out a treatment-group effect because of lack of power, a superiority of the synchronous combined carotid endarterectomy+CABG approach seems unlikely. Five-year follow-up of patients is still ongoing.CLINICAL TRIAL REGISTRATION: URL: https://www.controlled-trials.com. Unique identifier: ISRCTN13486906.
|
['Aged', 'Carotid Stenosis', 'Coronary Artery Bypass', 'Endarterectomy, Carotid', 'Female', 'Follow-Up Studies', 'Humans', 'Male', 'Middle Aged', 'Patient Safety', 'Treatment Outcome']
| 28,916,664
|
[['M01.060.116.100'], ['C10.228.140.300.200.360', 'C14.907.137.230', 'C14.907.253.123.360'], ['E04.100.376.719.332', 'E04.100.814.868.750', 'E04.928.220.520.220'], ['E04.100.814.456.250'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['N06.850.135.060.075.399'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Named Groups [M]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Hematoporphyrin derivative (Photofrin II) photosensitization of isolated mitochondria: impairment of anion translocation.
|
Isolated mitochondria have been incubated in the presence of 6 micrograms/ml hematoporphyrin derivative (Photofrin II), and irradiated at lambda = 365 nm. After 2 min irradiation (30 W/m2), a congruent to 50% inhibition of citric cycle intermediates transport is observed with a rather similar photosensitivity for the succinate, citrate or oxaloacetate carriers.
|
['Animals', 'Anions', 'Biological Transport', 'Carboxylic Acids', 'Dihematoporphyrin Ether', 'Hematoporphyrins', 'In Vitro Techniques', 'Intracellular Membranes', 'Mitochondria, Liver', 'Mitochondrial Swelling', 'Permeability', 'Rats']
| 2,948,508
|
[['B01.050'], ['D01.248.497.158'], ['G03.143'], ['D02.241'], ['D03.383.129.578.840.500.462.400.200', 'D03.633.400.909.500.462.400.200', 'D04.345.783.500.462.400.200', 'D23.767.727.462.400.200'], ['D03.383.129.578.840.500.462', 'D03.633.400.909.500.462', 'D04.345.783.500.462', 'D23.767.727.462'], ['E05.481'], ['A11.284.149.450', 'A11.284.835.514'], ['A11.284.430.214.190.875.564.461', 'A11.284.835.626.461'], ['G04.590'], ['G02.723'], ['B01.050.150.900.649.313.992.635.505.700']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[2 fatal cases of reaction due to the combination of MAO inhibitors and tricyclic antidepressants. Medico-legal aspects].
|
The authors describe two patients who died during hyperthermic reaction following the administration of MAO inhibitors and tricyclic antidepressants in therapeutic doses. In both cases, there was a high increase of temperature, rigidity of skeletal muscles and impairment consciousness with some analog with the syndrome following an overdose of the same drugs taken simultaneously, or with an acute intoxication by MAO inhibitors. The pharmacological aspects point out the synergistic mechanism between drugs of the two groups and the possible onset of the syndrome, not only after simultaneous administration, but also when the tricyclic antidepressants are prescribed without an adequate interval following a therapeutic cycle with MAO inhibitors. The possible professional responsibility is discussed and the authors emphasize that the pharmacological association must be used with caution and only after establishing the inefficacy of one single drug, starting anyhow with low doses, constantly looking after the patient and avoiding the parenteral administration.
|
['Adult', 'Antidepressive Agents, Tricyclic', 'Drug Synergism', 'Drug Therapy, Combination', 'Female', 'Humans', 'Jurisprudence', 'Middle Aged', 'Monoamine Oxidase Inhibitors']
| 7,170,585
|
[['M01.060.116'], ['D27.505.954.427.700.122.055'], ['G07.690.773.968.477'], ['E02.319.310'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['I01.880.604.583', 'N03.706.535'], ['M01.060.116.630'], ['D27.505.519.389.616']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 0
|
Active and passive fatigue in simulated driving: discriminating styles of workload regulation and their safety impacts.
|
Despite the known dangers of driver fatigue, it is a difficult construct to study empirically. Different forms of task-induced fatigue may differ in their effects on driver performance and safety. Desmond and Hancock (2001) defined active and passive fatigue states that reflect different styles of workload regulation. In 2 driving simulator studies we investigated the multidimensional subjective states and safety outcomes associated with active and passive fatigue. Wind gusts were used to induce active fatigue, and full vehicle automation to induce passive fatigue. Drive duration was independently manipulated to track the development of fatigue states over time. Participants were undergraduate students. Study 1 (N = 108) focused on subjective response and associated cognitive stress processes, while Study 2 (N = 168) tested fatigue effects on vehicle control and alertness. In both studies the 2 fatigue manipulations produced different patterns of subjective response reflecting different styles of workload regulation, appraisal, and coping. Active fatigue was associated with distress, overload, and heightened coping efforts, whereas passive fatigue corresponded to large-magnitude declines in task engagement, cognitive underload, and reduced challenge appraisal. Study 2 showed that only passive fatigue reduced alertness, operationalized as speed of braking and steering responses to an emergency event. Passive fatigue also increased crash probability, but did not affect a measure of vehicle control. Findings support theories that see fatigue as an outcome of strategies for managing workload. The distinction between active and passive fatigue is important for assessment of fatigue and for evaluating automated driving systems which may induce dangerous levels of passive fatigue.
|
['Adolescent', 'Adult', 'Automobile Driving', 'Fatigue', 'Female', 'Humans', 'Male', 'Safety', 'Stress, Psychological', 'Time Factors', 'Workload', 'Young Adult']
| 24,041,288
|
[['M01.060.057'], ['M01.060.116'], ['I03.125'], ['C23.888.369'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['N06.850.135.060.075'], ['F01.145.126.990', 'F02.830.900'], ['G01.910.857'], ['I03.946.225.500', 'N04.452.677.650.500'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Diseases [C]', 'Organisms [B]', 'Health Care [N]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 0
|
ATP8B1-mediated spatial organization of Cdc42 signaling maintains singularity during enterocyte polarization.
|
During yeast cell polarization localization of the small GTPase, cell division control protein 42 homologue (Cdc42) is clustered to ensure the formation of a single bud. Here we show that the disease-associated flippase ATPase class I type 8b member 1 (ATP8B1) enables Cdc42 clustering during enterocyte polarization. Loss of this regulation results in increased apical membrane size with scattered apical recycling endosomes and permits the formation of more than one apical domain, resembling the singularity defect observed in yeast. Mechanistically, we show that to become apically clustered, Cdc42 requires the interaction between its polybasic region and negatively charged membrane lipids provided by ATP8B1. Disturbing this interaction, either by ATP8B1 depletion or by introduction of a Cdc42 mutant defective in lipid binding, increases Cdc42 mobility and results in apical membrane enlargement. Re-establishing Cdc42 clustering, by tethering it to the apical membrane or lowering its diffusion, restores normal apical membrane size in ATP8B1-depleted cells. We therefore conclude that singularity regulation by Cdc42 is conserved between yeast and human and that this regulation is required to maintain healthy tissue architecture.
|
['Adenosine Triphosphatases', 'Animals', 'Cell Line', 'Cell Polarity', 'Enterocytes', 'Humans', 'Membrane Lipids', 'Mice', 'Phospholipid Transfer Proteins', 'Signal Transduction', 'cdc42 GTP-Binding Protein']
| 26,416,959
|
[['D08.811.277.040.025'], ['B01.050'], ['A11.251.210'], ['G04.250'], ['A03.556.124.369.290', 'A10.615.550.444.290', 'A11.436.290'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D10.570'], ['B01.050.150.900.649.313.992.635.505.500'], ['D12.776.157.674', 'D12.776.543.693'], ['G02.111.820', 'G04.835'], ['D08.811.277.040.330.300.400.700.050', 'D12.644.360.525.700.050', 'D12.776.157.325.515.700.050', 'D12.776.476.525.700.050']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Anatomy [A]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Characterization of porcine p53 and its regulation by porcine Mdm2.
|
Pigs have been increasingly recognized as a relevant model for studying many human diseases. However, functions and regulations of numerous critical molecules involved in human diseases are not well characterized in pigs, including the prominent tumor suppressor p53, a transcription factor involved in various anti-proliferative processes. In this study, we systematically characterized porcine p53 (p-p53) in its transcriptional activity and regulation by the E3 ligase Mdm2, in comparison with that of human p53 (h-p53). p-p53 is highly homologous to h-p53 with the N-terminal region showing relative divergence. p-p53 exhibits a comparable transcriptional activity to that of h-p53 towards a diverse range of known target genes, and is subject to ubiquitination and degradation by both human and porcine Mdm2 (h-/p-Mdm2). Utilization of the h-Mdm2 targeting compound Nutlin-3 and protein RPL11 inhibits the negative effect of p-Mdm2 on p-p53. These results suggest that the transcription activity and regulation of p-p53 is very similar to that of h-p53, and that the developed agents targeting the h-p53 pathway could be used in the study of p53 related processes and diseases in pigs.
|
['Animals', 'Cell Line', 'Gene Expression Regulation', 'Genes, p53', 'Humans', 'Mice', 'Proto-Oncogene Proteins c-mdm2', 'Swine']
| 32,334,023
|
[['B01.050'], ['A11.251.210'], ['G05.308'], ['G05.360.340.024.340.375.249.385', 'G05.360.340.024.340.415.400.385'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['B01.050.150.900.649.313.992.635.505.500'], ['D08.811.464.938.750.562', 'D12.776.624.664.700.185', 'D12.776.660.764'], ['B01.050.150.900.649.313.500.880']]
|
['Organisms [B]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Noninvasive testing of cerebral perfusion reserve prior to coronary artery bypass graft surgery.
|
Cerebral perfusion reserve testing using fluorine-18-fluoromethane and positron emission tomographic brain scanning to define cerebral blood flow abnormalities was performed in 5 patients being considered for combined coronary and carotid reconstructive surgery. Blood flow testing during normocapnia and following hypercapnia was utilized in these patients to determine the hemodynamic significance of known extracranial carotid artery occlusive lesions. Reserve diminution in 2 of these patients prompted combined surgery, whereas normal reserve values in the other 3 prompted coronary surgery alone. Results obtained in this preliminary series show how preoperative noninvasive testing of cerebral perfusion reserve adds to the diagnostic evaluation of patients with widespread vascular disease.
|
['Brain', 'Carbon Dioxide', 'Carotid Artery Diseases', 'Carotid Artery, External', 'Cerebral Angiography', 'Cerebrovascular Circulation', 'Coronary Artery Bypass', 'Coronary Disease', 'Fluorine Radioisotopes', 'Humans', 'Hydrocarbons, Fluorinated', 'Preoperative Care', 'Tomography, Emission-Computed']
| 3,132,064
|
[['A08.186.211'], ['D01.200.200', 'D01.362.150', 'D01.650.550.200'], ['C10.228.140.300.200', 'C14.907.253.123'], ['A07.015.114.186.200.210'], ['E01.370.350.578.937.180', 'E01.370.350.700.060.180', 'E01.370.350.700.560.180', 'E01.370.370.050.180', 'E01.370.376.537.750.180', 'E05.629.937.180'], ['G09.330.100.159'], ['E04.100.376.719.332', 'E04.100.814.868.750', 'E04.928.220.520.220'], ['C14.280.647.250', 'C14.907.585.250'], ['D01.496.749.340'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D02.455.526.510'], ['E02.760.795', 'E04.604.750', 'N02.421.585.795'], ['E01.370.350.350.800', 'E01.370.350.600.350.800', 'E01.370.350.710.800', 'E01.370.350.825.800', 'E01.370.384.730.800']]
|
['Anatomy [A]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Health Care [N]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Microbiological quality of raw and processed wild and cultured edible snails.
|
BACKGROUND: An increasing interest in snail farming in Greece and other European countries has been observed. Despite the fact that edible snails have been involved with problems of Salmonella spp. contamination, there are to our knowledge only limited studies regarding microbiological safety and hygiene of such products. Enumeration of microbial populations and presence/absence of Salmonella spp. in snail meat and intestines of wild Cornu aspersum, Helix lucorum and cultured Cornu aspersum snails from indoor/outdoor type farms was conducted. Furthermore, snail-processing steps were simulated in the laboratory and the population reduction in snail meat was determined.RESULTS: Microbial populations were higher in intestines than snail meat in almost all cases. Escherichia coli/coliforms and Enterococcus spp. populations were lower in the intestines and snail meat of cultured C. aspersum. Salmonella spp. were detected in the intestines and snail meat of wild snails only. The high levels of bacterial populations were considerably reduced after the appropriate processing.CONCLUSION: The lower populations of E. coli/coliforms, Enterococcus spp. and especially the absence of Salmonella spp. in cultured snails show that the controlled conditions decrease the possibility of pathogen presence and contribute to food safety and public health.
|
['Animals', 'Animals, Wild', 'Aquaculture', 'Bacterial Load', 'Bacterial Typing Techniques', 'Conus Snail', 'Cooking', 'Enterobacteriaceae', 'Enterococcus', 'Escherichia coli', 'Food Handling', 'Greece', 'Helix, Snails', 'Intestines', 'Mediterranean Islands', 'Microbial Viability', 'Salmonella', 'Shellfish']
| 24,122,749
|
[['B01.050'], ['B01.050.050.300'], ['J01.040.168'], ['E01.370.225.875.150.115', 'E01.370.225.875.220.115', 'E05.200.875.150.115', 'E05.200.875.220.115', 'G06.099.100'], ['E01.370.225.875.150.125', 'E05.200.875.150.125'], ['B01.050.500.644.400.275'], ['J01.576.423.200.200'], ['B03.440.450.425', 'B03.660.250.150'], ['B03.353.750.250.250', 'B03.510.550.250.250'], ['B03.440.450.425.325.300', 'B03.660.250.150.180.100'], ['J01.576.423.200'], ['Z01.542.383'], ['B01.050.500.644.400.750.450'], ['A03.556.124'], ['Z01.542.580.500', 'Z01.639.640'], ['G06.580'], ['B03.440.450.425.800', 'B03.660.250.150.710'], ['G07.203.300.600.875.700', 'J02.500.600.875.700']]
|
['Organisms [B]', 'Technology, Industry, and Agriculture [J]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Geographicals [Z]', 'Anatomy [A]']
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
|
CXCR2-modified CAR-T cells have enhanced trafficking ability that improves treatment of hepatocellular carcinoma.
|
Unlike hematological malignancies, solid tumors have proved to be less susceptible to chimeric antigen receptor (CAR)-T cell therapy, which is partially caused by reduced accumulation of therapeutic T cells in tumor site. Since efficient trafficking is the precondition and pivotal step for infused CAR-T cells to exhibit their anti-tumor function, strategies are highly needed to improve the trafficking ability of CAR-T cells for solid tumor treatment. Here, based on natural lymphocyte chemotaxis theory and characteristics of solid tumor microenvironments, we explored the possibility of enhancing CAR-T cell trafficking by using chemokine receptors. Our study found that compared with other chemokines, several CXCR2 ligands showed relatively high expression level in human hepatocellular carcinoma tumor tissues and cell lines. However, both human peripheral T cells and hepatocellular carcinoma tumor infiltrating T cells lacked expression of CXCR2. CXCR2-expressing CAR-T cells exhibited identical cytotoxicity but displayed significantly increased migration ability in vitro. In a xenograft tumor model, we found that expressing CXCR2 in CAR-T cells could significantly accelerate in vivo trafficking and tumor-specific accumulation, and improve anti-tumor effect of these cells.
|
['Animals', 'Carcinoma, Hepatocellular', 'Cell Line, Tumor', 'Cell Movement', 'Chemokine CCL2', 'Chemokine CXCL5', 'Cytotoxicity, Immunologic', 'Gene Expression', 'Humans', 'Immunotherapy, Adoptive', 'Interleukin-8', 'Liver Neoplasms', 'Mice', 'Receptors, Chimeric Antigen', 'Receptors, Interleukin-8B', 'T-Lymphocytes, Cytotoxic', 'Tumor Burden', 'Tumor Microenvironment', 'Xenograft Model Antitumor Assays']
| 31,981,231
|
[['B01.050'], ['C04.557.470.200.025.255', 'C04.588.274.623.160', 'C06.301.623.160', 'C06.552.697.160'], ['A11.251.210.190', 'A11.251.860.180'], ['G04.198', 'G07.568.500.180'], ['D12.644.276.374.200.110.990.600', 'D12.776.467.374.200.110.990.600', 'D23.125.300.110.990.600', 'D23.469.200.110.990.600', 'D23.529.374.200.110.990.500'], ['D12.644.276.374.200.120.250', 'D12.776.467.374.200.120.250', 'D23.125.300.120.250', 'D23.469.200.120.250', 'D23.529.374.200.120.250'], ['G12.287'], ['G05.297'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.095.465.425.400.330.050.400', 'E05.478.550.520.050.400'], ['D12.644.276.374.200.120.800', 'D12.644.276.374.465.312', 'D12.776.467.374.200.120.800', 'D12.776.467.374.465.246', 'D23.125.300.120.800', 'D23.469.200.120.800', 'D23.529.374.200.120.800', 'D23.529.374.465.312'], ['C04.588.274.623', 'C06.301.623', 'C06.552.697'], ['B01.050.150.900.649.313.992.635.505.500'], ['D12.776.543.750.655.500', 'D12.776.543.750.705.816.824.150', 'D12.776.826.387.500'], ['D12.776.543.750.695.160.500.750.750', 'D12.776.543.750.705.852.125.500.750.750', 'D12.776.543.750.705.852.420.421.750'], ['A11.118.637.555.283.875', 'A11.118.637.555.567.550.500.200', 'A11.118.637.555.567.569.220.200', 'A11.118.637.555.567.569.500.200', 'A15.145.229.637.555.283.875', 'A15.145.229.637.555.567.550.500.200', 'A15.145.229.637.555.567.569.220.200', 'A15.145.229.637.555.567.569.500.200', 'A15.382.490.555.283.875', 'A15.382.490.555.567.550.500.200', 'A15.382.490.555.567.569.220.200', 'A15.382.490.555.567.569.500.200'], ['E05.041.124.892'], ['G04.366.500'], ['E05.337.550.200.900', 'E05.624.850']]
|
['Organisms [B]', 'Diseases [C]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Case report on premature hair graying treated with Melitane 5% and oral hair supplements.
|
With chronological aging, hair turns gray. Untimely premature hair graying (PHG) may tremendously influence on cosmesis, self-credibility, and social life of the affected individuals. Consequently, early treatment is required to improve cosmetic appearance. To the best of our knowledge, until today, only one case of PHG is reported in the literature, and it occurred due to iron deficiency and successfully treated with ferrous sulfate. Herein, we delineate a case of PHG in a 14-year-old female treated with the topical formulation of Melitane 5% and oral hair supplements which resulted in boosting improvement in hair color.
|
['Administration, Topical', 'Adolescent', 'Dietary Supplements', 'Female', 'Hair Color', 'Humans', 'Treatment Outcome', 'alpha-MSH']
| 31,831,925
|
[['E02.319.267.120'], ['M01.060.057'], ['G07.203.300.456', 'J02.500.456'], ['G13.500', 'G16.690.490'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['D06.472.699.327.935.179', 'D06.472.699.327.935.531.750.050', 'D06.472.699.631.525.600.179', 'D06.472.699.631.525.600.531.750.050', 'D12.644.400.400.935.179', 'D12.644.400.400.935.531.750.050', 'D12.644.400.460.050', 'D12.644.548.365.935.179', 'D12.644.548.365.935.531.750.050', 'D12.644.548.691.525.690.179', 'D12.644.548.691.525.690.531.750.050', 'D12.776.631.650.405.935.179', 'D12.776.631.650.405.935.531.750.050', 'D12.776.631.650.460.050']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Named Groups [M]', 'Phenomena and Processes [G]', 'Technology, Industry, and Agriculture [J]', 'Organisms [B]', 'Health Care [N]', 'Chemicals and Drugs [D]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 1
| 0
|
Classifying single-trial EEG during motor imagery by iterative spatio-spectral patterns learning (ISSPL).
|
In most current motor-imagery-based brain-computer interfaces (BCIs), machine learning is carried out in two consecutive stages: feature extraction and feature classification. Feature extraction has focused on automatic learning of spatial filters, with little or no attention being paid to optimization of parameters for temporal filters that still require time-consuming, ad hoc manual tuning. In this paper, we present a new algorithm termed iterative spatio-spectral patterns learning (ISSPL) that employs statistical learning theory to perform automatic learning of spatio-spectral filters. In ISSPL, spectral filters and the classifier are simultaneously parameterized for optimization to achieve good generalization performance. A detailed derivation and theoretical analysis of ISSPL are given. Experimental results on two datasets show that the proposed algorithm can correctly identify the discriminative frequency bands, demonstrating the algorithm's superiority over contemporary approaches in classification performance.
|
['Algorithms', 'Artificial Intelligence', 'Brain', 'Brain Mapping', 'Electroencephalography', 'Evoked Potentials, Motor', 'Humans', 'Imagination', 'Movement', 'Pattern Recognition, Automated']
| 18,714,838
|
[['G17.035', 'L01.224.050'], ['G17.035.250', 'L01.224.050.375'], ['A08.186.211'], ['E01.370.350.578.875.500', 'E01.370.376.537.625.500', 'E05.629.875.500'], ['E01.370.376.300', 'E01.370.405.245'], ['G07.265.216.500.385', 'G11.561.200.500.385'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F02.463.188.634'], ['G07.568', 'G11.427.410'], ['L01.399.750']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Psychiatry and Psychology [F]']
| 1
| 1
| 0
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Effect of cadmium on physiological parameters of cereal and millet plants-A comparative study.
|
Metal load is an abiotic stress that becomes stronger by continual industrial production, wastage, and long-range transport of contaminants. It deteriorates the conditions of agricultural soil that leads to lower growth of cereals as well as decreasing nutritional value of harvested grains. Cadmium (Cd) entry by food chain also affects the health of population. The present study is focused on finding out the superior cereal variety under increasing Cd regime. The plants were grown in increasing Cd levels (0-1000 µM) in the medium and were investigated on 15th day of the exposure. Various parameters like antioxidative enzymes and osmoprotectant levels were studied in both roots and shoots. Cd accumulation in plant organs was determined by atomic absorption spectrophotometry (AAS). Analysis of stress tolerance mechanisms through reactive oxygen species (ROS) scavenging and better partitioning of Cd in roots indicated kodo millet to be more stress tolerant than wheat.
|
['Biodegradation, Environmental', 'Cadmium', 'Paspalum', 'Soil Pollutants', 'Species Specificity', 'Stress, Physiological', 'Triticum']
| 27,420,113
|
[['N06.230.080.600.500', 'N06.850.460.375.500'], ['D01.268.556.137', 'D01.268.956.061', 'D01.552.544.137'], ['B01.650.940.800.575.912.250.822.744'], ['D27.888.284.756'], ['G16.824'], ['G07.775'], ['B01.650.940.800.575.912.250.822.918']]
|
['Health Care [N]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
In vitro studies on interaction of 4-hydroperoxyifosfamide and 2-mercaptoethanesulphonate in malignant gliomas.
|
Drug interference of ifosfamide and sodium 2-mercaptoethanesulphonate (MESNA) was studied in three malignant glioma cell cultures (HTZ-17, HTZ-209B, and HTZ-243) by a recently developed in vitro method for evaluation of multimodal treatment interactions. Glioma cell cultures were treated in monolayer 96-well tissue-culture plates for 2 h each, with 4-hydroperoxyifosfamide and MESNA combined in both sequences, or alone. Concentrations ranged from 0.01 microM to 50 microM in single-modality exposures, and from 0.01 microM to 10 microM in combination exposures. After five population doubling times, DNA synthesis was determined by a standard [3H]Tdr-incorporation liquid-scintillation-counting protocol. Data points were evaluated for mono- and combined treatment dose effects (adapted with a probit function), and a model-free three-dimensional response surface was created that was compared to the theoretical additive, anticipated response surface. Local additivity was analysed for any ratio of combined treatment. No tumour effects were seen with MESNA in single-drug exposure, whereas ifosfamide resulted in more than 90% inhibition of tumour DNA synthesis. In combination experiments, MESNA could be confirmed to be inert: the anticipated theoretical combination response surfaces formed a three-dimensional extension of the single-drug ifosfamide dose/response curves--the experimental combination response surfaces displayed an identical appearance (P < or = 0.05). In conclusion, these results indicate no drug interference of MESNA and ifosfamide in malignant glioma cells.
|
['Antineoplastic Agents', 'Antineoplastic Combined Chemotherapy Protocols', 'Combined Modality Therapy', 'Drug Interactions', 'Drug Screening Assays, Antitumor', 'Glioma', 'Humans', 'Ifosfamide', 'Mesna', 'Models, Biological', 'Tumor Cells, Cultured']
| 8,408,185
|
[['D27.505.954.248'], ['E02.183.750.500', 'E02.319.077.500', 'E02.319.310.037'], ['E02.186'], ['G07.690.773.968'], ['E01.370.225.500.388', 'E05.200.500.388', 'E05.242.417', 'E05.337.550.200'], ['C04.557.465.625.600.380', 'C04.557.470.670.380', 'C04.557.580.625.600.380'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D02.455.526.728.650.730.243.250', 'D02.705.672.500.243.250', 'D03.383.533.500'], ['D02.455.326.146.100.050.490', 'D02.886.489.590', 'D02.886.645.600.055.050.500'], ['E05.599.395'], ['A11.251.860']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Organisms [B]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
In vivo blood metal ion levels in patients after total shoulder arthroplasty.
|
BACKGROUND: Products from metal wear have been identified as a potential cause of adverse local tissue reactions and implant failure in total hip arthroplasty. However, the role of metal ion exposure in patients after total shoulder replacement is unclear. The objective of the present study was to determine in vivo blood metal ion levels of cobalt, chromium, and titanium in patients after anatomic total shoulder arthroplasty (TSA) or reverse TSA.METHODS: A consecutive series of patients after anatomic TSA or reverse TSA was evaluated retrospectively. After exclusion of patients with additional metal implants, 40 patients with unilateral anatomic TSA (n = 20) or reverse TSA (n = 20) were available for whole-blood metal ion analysis at a mean follow-up of 28 ± 9.6 months. Twenty-three healthy individuals without metal implants served as a control group.RESULTS: Mean cobalt ion concentrations were 0.18 µg/L (range, 0.1-0.66 µg/L), 0.15 µg/L (range, 0.03-0.48 µg/L), and 0.11 µg/L (range, 0.03-0.19 µg/L), mean chromium ion levels were 0.48 µg/L (range, 0.17-2.41 µg/L), 0.31 µg/L (range, 0.09-1.26 µg/L), and 0.14 µg/L (range, 0.04-0.99 µg/L), and mean titanium ion concentrations were 1.31 µg/L (range, 0.75-4.52 µg/L), 0.84 µg/L (range, 0.1-1.64 µg/L), and 0.62 µg/L (range, 0.32-2.14 µg/L) in the reverse TSA group, the anatomic TSA group, and the control group, respectively.CONCLUSIONS: TSA resulted in elevated metal ion levels compared with healthy controls, although overall metal ion concentrations measured in this study were relatively low. The role of local metal ion exposure in patients with total shoulder replacements should be further investigated.
|
['Aged', 'Aged, 80 and over', 'Arthroplasty, Replacement, Shoulder', 'Case-Control Studies', 'Chromium', 'Cobalt', 'Cross-Sectional Studies', 'Female', 'Follow-Up Studies', 'Humans', 'Ions', 'Male', 'Middle Aged', 'Prosthesis Design', 'Retrospective Studies', 'Shoulder Prosthesis', 'Titanium']
| 30,518,478
|
[['M01.060.116.100'], ['M01.060.116.100.080'], ['E04.555.110.110.299', 'E04.650.110.299', 'E04.680.101.110.299'], ['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['D01.268.556.175', 'D01.268.956.124', 'D01.552.544.175'], ['D01.268.556.185', 'D01.268.956.155', 'D01.552.544.185'], ['E05.318.372.500.875', 'N05.715.360.330.500.875', 'N06.850.520.450.500.875'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D01.248.497'], ['M01.060.116.630'], ['E05.320.550', 'E07.695.680'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E07.695.400.852'], ['D01.268.557.800', 'D01.268.956.878', 'D01.552.547.800']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Chemicals and Drugs [D]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
[Therapeutic effects of Lamivudine in combination with Thymopentin on chronic hepatitis B].
|
OBJECTIVE: To explore the efficacy of anti-viral drug in combination with immunoregulatory agent in treatment of chronic hepatitis B.METHODS: Totally 98 patients with chronic hepatitis B were divided at random into 3 groups. In groups A (42 cases) lamivudine was used in combination with thymopentin to treat chronic hepatitis B. Lamivudine or thymopentin was used alone in groups B (38 cases) and C (18 cases), respectively. The dynamic changes in serum parameters reflecting HBV replication and liver function were observed.RESULTS: At the end of the treatments, the rates of negative conversion of HBeA g and positive conversion of anti-HBe in the serum were significantly higher in group A than in group B (P<0.05). There was no significant difference between group A and group C (P>0.05). The rate of negative conversion of HBV DNA was markedly higher in group A than in group C (P<0.05). However, there was no remarkable difference between group A and group B (P>0.05). The changes in parameters of viral replication in 6 and 12 months after the treatments were not significantly different from those at the end of the treatments. The effective rate and total effective rate were markedly higher in group A than in the ther 2 groups. Meanwhile, the rate of ALT normalization of remained higher than 85% in group A.CONCLUSIONS: Lamivudine in combination with thymopentin can exert great and lasting effects on HBV and is effective in normalization of ALT.
|
['Adjuvants, Immunologic', 'Adult', 'Antiviral Agents', 'Drug Therapy, Combination', 'Female', 'Hepatitis B virus', 'Hepatitis B, Chronic', 'Humans', 'Lamivudine', 'Male', 'Middle Aged', 'Thymopentin', 'Treatment Outcome']
| 12,665,914
|
[['D27.505.696.477.067'], ['M01.060.116'], ['D27.505.954.122.388'], ['E02.319.310'], ['B04.280.375.650.425', 'B04.450.390.650.425'], ['C01.221.250.500.100', 'C01.925.256.430.400.100', 'C01.925.440.435.100', 'C06.552.380.350.100', 'C06.552.380.705.437.100'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D03.383.742.680.245.500.950.500', 'D13.570.230.329.950.500', 'D13.570.230.500.925.500', 'D13.570.685.245.500.950.500'], ['M01.060.116.630'], ['D06.472.910.800.850'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Chemicals and Drugs [D]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Diseases [C]', 'Health Care [N]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Time-Resolved Spectroscopic Study of the Defluorination and Cyclization Reactions of Lomefloxacin in Water.
|
The mechanism of the defluorination reaction(s) of lomefloxacin (LF) upon light illumination was investigated by using ultrafast laser flash photolysis combined with transient resonance Raman spectroscopy in near neutral water solution. The zwitterionic configuration of LF was determined to be the main species present in the near neutral water solution and was the species that was photoexcited to initiate the photochemical reaction. Femtosecond transient absorption revealed that the first excited singlet state (S1) of LF did not appreciably undergo intersystem crossing (ISC), and instead partially decayed to the ground state via fluorescence emission, and there was partial cleavage of the carbon-fluorine bond at position 8 to produce a singlet LF aryl cation intermediate. The transient resonance Raman results provided a direct observation and vibrational spectral characterization of the singlet LF aryl cation species. Subsequently, the transformation from the singlet LF aryl cation to a triplet carbene via an ISC process was seen in nanosecond transient absorption spectra. Finally, the triplet carbene experienced a cyclization reaction with the N-ethyl chain to form a tricyclic product.
|
['Cyclization', 'Fluoroquinolones', 'Halogenation', 'Lasers', 'Photolysis', 'Spectrum Analysis, Raman', 'Time Factors', 'Water']
| 28,332,403
|
[['G02.111.180', 'G02.607.133', 'G03.208'], ['D03.633.100.810.835.322'], ['G02.111.323', 'G03.360'], ['E07.632.490', 'E07.710.520'], ['G02.740.685'], ['E05.196.822.860', 'E05.196.867.890'], ['G01.910.857'], ['D01.045.250.875', 'D01.248.497.158.459.650', 'D01.650.550.925']]
|
['Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Delivery of perioperative chemotherapy for bladder cancer in routine clinical practice.
|
BACKGROUND: Few articles have documented regimens and timing of perioperative chemotherapy for bladder cancer in routine practice. Here, we describe practice patterns in the general population of Ontario, Canada.METHODS: In this retrospective cohort study, treatment and physician billing records were linked to the Ontario Cancer Registry to describe use of neoadjuvant (NACT) and adjuvant (ACT) chemotherapy among all patients with muscle-invasive bladder cancer treated with cystectomy in Ontario 1994-2008. Time to initiation of ACT (TTAC) was measured from cystectomy. Multivariate Cox regression was used to identify factors associated with overall (OS) and cancer-specific survival (CSS).RESULTS: Of 2944 patients undergoing cystectomy, 4% (129/2944) and 19% (571/2944) were treated with NACT and ACT, respectively. Five-year OS was 25% [95% confidence interval (CI) 17% to 34%] for NACT, 29% (95% CI 25% to 33%) for ACT cases. Among patients with identifiable drug regimens, cisplatin was used in 82% (253/308) and carboplatin in 14% (43/308). The most common regimens were gemcitabine-cisplatin (54%, 166/308) and methotrexate, vinblastine, doxorubicin, cisplatin (MVAC) (21%, 66/308). Mean TTAC was 10 weeks; 23% of patients had TTAC >12 weeks. TTAC >12 weeks was associated with inferior OS [hazard ratio (HR) 1.28, 95% CI 1.00-1.62] and CSS (HR 1.30, 95% CI 1.00-1.69). In adjusted analyses, OS and CSS were lower among patients treated with carboplatin compared with those treated with cisplatin; OS HR 2.14 (95% CI 1.40-3.29) and CSS HR 2.06 (95% CI 1.26-3.37).CONCLUSIONS: Most patients in the general population receive cisplatin, and this may be associated with superior outcomes to carboplatin. Initiation of ACT beyond 12 weeks is associated with inferior survival. Patients should start ACT as soon as they are medically fit to do so.
|
['Adult', 'Aged', 'Aged, 80 and over', 'Antineoplastic Combined Chemotherapy Protocols', 'Canada', 'Carboplatin', 'Chemotherapy, Adjuvant', 'Cisplatin', 'Cohort Studies', 'Cystectomy', 'Deoxycytidine', 'Doxorubicin', 'Female', 'Humans', 'Male', 'Methotrexate', 'Middle Aged', 'Neoadjuvant Therapy', 'Retrospective Studies', 'Treatment Outcome', 'Urinary Bladder', 'Urinary Bladder Neoplasms', 'Vinblastine', 'Young Adult']
| 24,915,872
|
[['M01.060.116'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['E02.183.750.500', 'E02.319.077.500', 'E02.319.310.037'], ['Z01.107.567.176'], ['D02.257.125'], ['E02.186.170', 'E02.319.170'], ['D01.210.375', 'D01.625.125', 'D01.710.100'], ['E05.318.372.500.750', 'N05.715.360.330.500.750', 'N06.850.520.450.500.750'], ['E04.950.774.150'], ['D03.383.742.680.245.500', 'D13.570.230.329', 'D13.570.685.245.500'], ['D02.455.426.559.847.562.050.200.175', 'D04.615.562.050.200.175', 'D09.408.051.059.200.175'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D03.633.100.733.631.192.500'], ['M01.060.116.630'], ['E02.186.450'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['A05.810.890'], ['C04.588.945.947.960', 'C12.758.820.968', 'C12.777.829.813', 'C13.351.937.820.945', 'C13.351.968.829.707'], ['D03.132.436.681.827.650', 'D03.633.100.473.402.681.827.650', 'D03.633.100.496.500.500.681.827.650'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Geographicals [Z]', 'Chemicals and Drugs [D]', 'Health Care [N]', 'Organisms [B]', 'Anatomy [A]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Monte Carlo calculations applied to the parametrical studies in a whole body counter.
|
The use of a Monte Carlo code for the analysis and interpretation of whole body counting measurements is described. The sources of error are analysed and commented to show how a counting geometry can be improved by improving accuracy and precision in a measurement. The effects of body size, contamination distribution and counting geometry are also parameters which can be easily used to improve the quality of a body burden assessment. The optimisation of the detector (position, shielding, shape and size) is also commented on the basis of calculations in the photon energy range usually encountered in routine measurements. The results obtained from these simulations are confirmed by experimental results.
|
['Humans', 'Monte Carlo Method', 'Phantoms, Imaging', 'Scattering, Radiation', 'Whole-Body Counting']
| 17,526,910
|
[['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.740.525', 'L01.906.394.422', 'N05.715.360.750.540', 'N06.850.520.830.525'], ['E07.671'], ['E05.196.822', 'G01.867'], ['E05.799.950']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Information Science [L]', 'Health Care [N]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 1
| 0
|
Rapid detection of trace bacteria in biofluids using porous monoliths in microchannels.
|
We present advancements in microfluidic technology for rapid detection of as few as 10 rickettsial organisms in complex biological samples. An immuno-reactive filter, macroporous polyacrylamide monolith (PAM), fabricated within a microfluidic channel enhances solid-phase immuno-capture, staining and detection of targeted bacteria. Bacterial cells in samples flowing through the channel are forced to interact with the PAM filter surface due to size exclusion, overcoming common transport and kinetic limitations for rapid (min), high-efficiency (~100%) capture. In the process, targeted cells in sample volumes of 10 ìl to >100 ìl are concentrated within a sub-50 nl region at the PAM filter edge in the microchannel, thus concentrating them over 1000-fold. This significantly increases sensitivity, as the hydrophilic PAM also yields low non-specific immuno-fluorescence backgrounds with samples including serum, blood and non-targeted bacteria. The concentrated target cells are detected using fluorescently-labeled antibodies. With a single 2.0?2.0?0.3 mm PAM filter, as few as 10 rickettsial organisms per 100 µl of lysed blood sample can be analyzed within 60 min, as compared to hours or even days needed for conventional detection methods. This method is highly relevant to rapid, multiplexed, low-cost point of care diagnostics at early stages of infection where diagnostics providing more immediate and actionable test results are needed to improve patient outcomes and mitigate potential natural and non-natural outbreaks or epidemics of rickettsial diseases.
|
['Acrylic Resins', 'Biosensing Techniques', 'Equipment Design', 'Humans', 'Microfluidic Analytical Techniques', 'Porosity', 'Rickettsia typhi', 'Sensitivity and Specificity', 'Typhus, Endemic Flea-Borne']
| 24,316,449
|
[['D05.750.716.822.111', 'D25.720.716.822.111', 'J01.637.051.720.716.822.111'], ['E05.601.043'], ['E05.320'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.588.465'], ['G01.374.710'], ['B03.660.050.783.875.650.650.810'], ['E05.318.370.800', 'E05.318.740.872', 'G17.800', 'N05.715.360.325.700', 'N05.715.360.750.725', 'N06.850.520.445.800', 'N06.850.520.830.872'], ['C01.150.252.400.789.725.800', 'C01.920.914.725.800']]
|
['Chemicals and Drugs [D]', 'Technology, Industry, and Agriculture [J]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
|
Brain Connectivity and Cognitive Flexibility in Nonirradiated Adult Survivors of Childhood Leukemia.
|
Background: This study aimed to assess functional and structural brain connectivity in adult childhood leukemia survivors and the link with cognitive functioning and previously identified risk factors such as intrathecal methotrexate dose and age at start of therapy.Methods: Thirty-one nonirradiated adult childhood leukemia survivors and 35 controls underwent cognitive testing and multimodal magnetic resonance imaging (resting state functional MRI, T1-weighted, diffusion-weighted, and myelin water imaging [MWI]). Analyses included dual regression, voxel-based morphometry, advanced diffusion, and MWI modeling techniques besides stepwise discriminant function analysis to identify the most affected executive cognitive domain. Correlations with discrete intrathecal MTX doses and (semi)continuous variables were calculated using Spearman's rank and Pearson's correlation, respectively. All correlation tests were two-sided. Positive and negative T-contrasts in functional and structural MRI analysis were one-sided.Results: Survivors demonstrated lower functional connectivity between the default mode network (DMN) and inferior temporal gyrus (ITG; P < .008). Additionally, we observed higher fractional anisotropy (FA; P = .04) and lower orientation dispersion index (ODI; P = .008) at the left centrum semiovale, which could-given that several fiber bundles cross this region-suggest selective reduced integrity of the respective white matter tracts. Set shifting reaction time, a measure of cognitive flexibility, was mostly impaired and correlated with lower FA (r = -0.53, P = .003) and higher ODI (r = 0.40, P = .04) in survivors but not with DMN-ITG connectivity. There were no statistically significant differences between survivors and controls in WM or GM volume, nor was there a statistically significant correlation between imaging measurements and age at start of therapy or intrathecal methotrexate dose.Conclusions: Adult, nonirradiated childhood leukemia survivors show altered brain connectivity, which is linked with cognitive flexibility.
|
['Adolescent', 'Adult', 'Age of Onset', 'Brain', 'Cancer Survivors', 'Case-Control Studies', 'Child', 'Cognition', 'Female', 'Humans', 'Injections, Spinal', 'Leukemia', 'Magnetic Resonance Imaging', 'Male', 'Methotrexate', 'Nerve Net', 'Neuronal Plasticity', 'White Matter', 'Young Adult']
| 29,514,304
|
[['M01.060.057'], ['M01.060.116'], ['N05.715.350.075.100', 'N06.850.490.250.100'], ['A08.186.211'], ['M01.860.350'], ['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['M01.060.406'], ['F02.463.188'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.319.267.530.580'], ['C04.557.337'], ['E01.370.350.825.500'], ['D03.633.100.733.631.192.500'], ['A08.511'], ['G11.561.638'], ['A08.186.211.204', 'A08.186.854.880'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Health Care [N]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Psychiatry and Psychology [F]', 'Organisms [B]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
[Weekly Taxol (Paclitaxel) administration in the second-line treatment of breast cancer].
|
Based on phase II and III trials Taxol administered in the form of three times/week 1 or 3 hr infusion as mono-or combined chemotherapy of breast cancer is an effective treatment option. These studies proved that this form of drug delivery is effective and well tolerated and the overall response rate is around 50%. The 1 hr weekly infusion of Taxol is an effective second-line treatment in metastatic breast cancer and is better than the 3-weekly infusion since the decreased toxicity increases the therapeutic index.
|
['Antineoplastic Agents, Phytogenic', 'Breast Neoplasms', 'Drug Administration Schedule', 'Female', 'Humans', 'Paclitaxel']
| 12,202,898
|
[['D27.505.954.248.179'], ['C04.588.180', 'C17.800.090.500'], ['E02.319.283'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D02.455.426.392.368.242.888.777', 'D02.455.849.291.850.777']]
|
['Chemicals and Drugs [D]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Characterization of ovarian membrane receptor for 17,20beta-dihydroxy-4-pregnen-3-one, a maturation-inducing hormone in yellowtail, Seriola quinqueradiata.
|
In our previous studies, we tentatively identified 17,20 beta-dihydroxy-4-pregnen-3-one (17,20 beta-P) as a maturation-inducing hormone (MIH) in yellowtail (Seriola quinqueradiata) through in vivo and in vitro experiments. In this study, we investigated the binding sites for radioactive 17,20 beta-P and characterized the receptor binding to the ovarian plasma membrane in yellowtail undergoing first stage of maturation (FSM). Equilibrium binding sites for 17,20 beta-P have been detected within 1h incubation and the binding dissociated completely within 50 min at 4 degrees C and was pH dependent (optimum pH 7.8). Scatchard analyses of specifically bound 17,20 beta-P showed the evidence of a single class of high affinity binding sites (K(D)=22.9 nM), with limited capacity (B(max)=2.1 pmol/g tissue) to the ovarian membrane of yellowtail undergoing FSM. Competition results revealed that ovarian membrane receptor was highly specific for 17,20 beta-P. There was no other steroid competed strongly with the binding sites of [3H]17,20 beta-P, except 17,20 beta-P itself. On the other hand, 17,20 beta-P did not bind to the membrane prepared from maturationally incompetent (MI) and ovulation (OV) stages of oocytes. As the time proceeded after the stimulation of HCG, binding activity increased significantly (0.389+/-0.036 pmol/g tissue) in the ovarian membrane of maturationally competent (MC) oocytes by 12h postinjection. The binding activity was further significant (0.868+/-0.032 pmol/g tissue) at FSM by 24h postinjection and reached its peak (0.920+/-0.115 pmol/g tissue) temporarily at second stage of maturation (SSM) by 36 h postinjection and then sharply declined to the prestimulation levels during OV stage by 48 h postinjection. In addition to our previous findings, the present results indicate that 17,20 beta-P is the MIH in yellowtail.
|
['Animals', 'Binding Sites', 'Binding, Competitive', 'Cell Membrane', 'Chorionic Gonadotropin', 'Female', 'Hydrogen-Ion Concentration', 'Hydroxyprogesterones', 'Male', 'Oocytes', 'Ovary', 'Perciformes', 'Receptors, Cell Surface', 'Tritium']
| 12,161,204
|
[['B01.050'], ['G02.111.570.120'], ['E05.196.080', 'G02.111.084', 'G02.111.570.120.309'], ['A11.284.149'], ['D06.472.699.322.326', 'D06.472.699.649.367', 'D12.644.548.726.367', 'D12.776.780.400'], ['G02.300'], ['D04.210.500.745.745.654.829.395', 'D06.472.334.851.687.750.478'], ['A05.360.490.690.680', 'A11.497.497.600'], ['A05.360.319.114.630', 'A05.360.576.497', 'A06.300.312.497'], ['B01.050.150.900.493.602'], ['D12.776.543.750'], ['D01.268.406.875', 'D01.362.340.875', 'D01.496.749.925']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Chemicals and Drugs [D]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Cystic fibrosis in a child with situs inversus, a distinctively unusual association.
|
Cystic fibrosis is the most frequently witnessed potentially lethal autosomal recessive, genetic disease but its incidence is extremely low in South-Asian population. We report a case Cystic Fibrosis in a patient with Situs inversus, a condition not witnessed in medical literature of Pakistan or more captivatingly even Asia. The patient was a three and half years old male child presenting with a history of fever, cough and jaundice. Physical examination lead to the initial diagnosis of situs inversus, which was confirmed by the chest radiograph, echocardiography and ultrasound of abdomen. Further evaluations were conducted to establish the cause of respiratory symptoms. The findings of pansinusitis as evidenced by the radiography and an exceedingly high sweat chloride concentration of 100 mmol/L resulted in the conclusive diagnosis of Cystic Fibrosis.
|
['Child, Preschool', 'Cystic Fibrosis', 'Humans', 'Male', 'Situs Inversus']
| 20,361,688
|
[['M01.060.406.448'], ['C06.689.202', 'C08.381.187', 'C16.320.190', 'C16.614.213'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C16.131.810']]
|
['Named Groups [M]', 'Diseases [C]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Chronic L-NAME hypertension in rats and autoregulation of juxtamedullary preglomerular vessels.
|
The impact of chronic NG-nitro-L-arginine methyl ester (L-NAME)-induced hypertension (20 mg.kg-1.day-1 po, for 25 days) on pressure responsiveness was assessed in vessels ranging from arcuate arteries (ArcA) to juxtaglomerular afferent arterioles (JAA), using videomicroscopy and blood-perfused juxtamedullary nephron (JMN) preparations. Respective tail-cuff pressures of control and L-NAME rats were 127 +/- 2 (n = 8) and 173 +/- 4 mmHg (n = 5). Corresponding vessels of both groups had similar calibers at 60 mmHg. Increasing blood perfusion pressure to 200 mmHg constricted control ArcA and JAA by 26 +/- 4% (n = 20) and 43 +/- 5% (n = 15), respectively. Instead, a respective 3 +/- 4% (n = 15) and 21 +/- 9% (n = 6) pressure-induced dilation occurred in L-NAME vessels, and 86 +/- 2% of glomeruli expressed alpha-smooth muscle actin. Responses to acetylcholine (1 microM) but not to nitroprusside (1 mM) were impaired by L-NAME. Maximal relaxation induced by Mn2+ (10 mM) revealed equal basal tone and similar passive viscoelastic properties in control and L-NAME vessels. No vascular hypertrophy was found in L-NAME vessels. Chronic L-NAME hypertension is therefore associated with a selective loss of vascular autoregulation in JMNs, which may contribute to glomerular injury.
|
['Actins', 'Animals', 'Arginine', 'Blood Pressure', 'Blood Vessels', 'Chronic Disease', 'Hemodynamics', 'Homeostasis', 'Hypertension', 'Juxtaglomerular Apparatus', 'Male', 'Muscle, Smooth, Vascular', 'NG-Nitroarginine Methyl Ester', 'Nitric Oxide', 'Rats', 'Rats, Wistar', 'Renal Circulation']
| 7,653,592
|
[['D05.750.078.730.250', 'D12.776.210.500.100', 'D12.776.220.525.255'], ['B01.050'], ['D12.125.068.050', 'D12.125.095.104', 'D12.125.142.087'], ['E01.370.600.875.249', 'G09.330.380.076'], ['A07.015'], ['C23.550.291.500'], ['G09.330.380'], ['G07.410'], ['C14.907.489'], ['A05.810.453.324.359.520', 'A05.810.453.736.520.520'], ['A02.633.570.491', 'A07.015.733.500', 'A10.690.467.491'], ['D12.125.068.050.525', 'D12.125.095.104.525'], ['D01.339.387', 'D01.625.550.500', 'D01.625.700.500', 'D01.650.550.587.600'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.900'], ['G08.852.725', 'G09.330.100.812']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Outcomes after surgical treatment of missed Monteggia fractures in children.
|
BACKGROUND: Chronic dislocation of the radial head treatment in Monteggia fracture dislocation is still controversial. We present a large series of patients treated in our Institution.MATERIALS AND METHODS: The outcome of 22 children treated surgically between 1988 and 2011 for post-traumatic chronic radial head dislocation is reported. There were 12 girls and 10 boys with a mean age at surgery of 7.2 years (4.1-13.6). The mean interval between injury and treatment was 15.7 months (1-128). Nine patients underwent open reduction with removal of interposed tissue and repair (7) or Bell-Tawse reconstruction (2) of the annular ligament. Ten patients underwent osteotomy, gradual lengthening and angulation of the ulna by external fixation. Two patients underwent angular osteotomy of the proximal ulna with open wedge, open reduction in the radial head and reconstruction of the annular ligament. One patient admitted to the hospital 10 years after injury underwent radial head excision at 13.7 years of age.RESULTS: After a mean follow-up of 5.5 years (1-24.3), the radial head stayed reduced in 15 patients and subluxated in 5. In one case, redislocation occurred. All patients but five were pain-free. The elbow performance score (Kim score) was excellent in 14 cases, good in four and fair in four, with a mean score of 91, corresponding to a good result. Complications included a transient posterior interosseus nerve palsy (1), and one non-union of the ulna.DISCUSSION AND CONCLUSION: Chronic Monteggia lesions must be treated. The clinical outcomes are usually better than the congruency of the radiocapitellar joint.
|
['Adolescent', 'Child', 'Child, Preschool', 'Female', 'Humans', 'Male', "Monteggia's Fracture", 'Radiography', 'Treatment Outcome']
| 25,957,548
|
[['M01.060.057'], ['M01.060.406'], ['M01.060.406.448'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C05.550.518.336.750', 'C26.289.336.750', 'C26.404.937.547'], ['E01.370.350.700'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Named Groups [M]', 'Organisms [B]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Deficits in cognitive function and hippocampal plasticity in GM2/GD2 synthase knockout mice.
|
In this study, we used GM2/GD2 synthase knockout (GM2/GD2?/?) mice to examine the influence of deficiency in ganglioside “a-pathway” and “b-pathway” on cognitive performances and hippocampal synaptic plasticity. Eight-week-old GM2/GD2?/? male mice showed a longer escape-latency in Morris water maze test and a shorter latency in step-down inhibitory avoidance task than wild-type (WT) mice. Schaffer collateral-CA1 synapses in the hippocampal slices from GM2/GD2?/? mice showed an increase in the slope of EPSPs with reduced paired-pulse facilitation, indicating an enhancement of their presynaptic glutamate release. In GM2/GD2?/? mice, NMDA receptor (NMDAr)-dependent LTP could not be induced by high-frequency (100–200 Hz) tetanus or è-burst conditioning stimulation (CS), whereas NMDAr-independent LTP was induced by medium-frequency CS (20–50 Hz). The application of mono-sialoganglioside GM1 in the slice from GM2/GD2?/? mice, to specifically recover the a-pathway, prevented the increased presynaptic glutamate release and 20 Hz-LTP induction, whereas it could not rescue the impaired NMDAr-dependent LTP. These findings suggest that b-pathway deficiency impairs cognitive function probably through suppression of NMDAr-dependent LTP, while a-pathway deficiency may facilitate NMDAr-independent LTP through enhancing presynaptic glutamate release. As both of the NMDAr-independent LTP and increased presynaptic glutamate release were sensitive to the blockade of L-type voltage-gated Ca2+ channels (L-VGCC), a-pathway deficiency may affect presynaptic L-VGCC.
|
['Animals', 'Avoidance Learning', 'CA1 Region, Hippocampal', 'Calcium', 'Cognition Disorders', 'Electric Stimulation', 'Excitatory Postsynaptic Potentials', 'G(M1) Ganglioside', 'In Vitro Techniques', 'Long-Term Potentiation', 'Male', 'Maze Learning', 'Memory Disorders', 'Mice', 'Mice, Knockout', 'N-Acetylgalactosaminyltransferases', 'Neuronal Plasticity', 'Receptors, N-Methyl-D-Aspartate', 'Synapses', 'Synaptic Transmission']
| 24,765,676
|
[['B01.050'], ['F02.463.425.097', 'F02.463.785.373.173'], ['A08.186.211.180.405.099', 'A08.186.211.200.885.287.500.345.099'], ['D01.268.552.100', 'D01.552.539.288', 'D23.119.100'], ['F03.615.250'], ['E05.723.402'], ['G04.580.887.249', 'G07.265.675.887.249', 'G07.265.880.750.199', 'G11.561.570.918.249', 'G11.561.830.750.199'], ['D09.400.410.420.025.475.390', 'D10.390.470.025.475.390', 'D10.570.877.360.025.475.390'], ['E05.481'], ['G11.561.638.350'], ['F02.463.425.874.500'], ['C10.597.606.525', 'C23.888.592.604.529', 'F01.700.625'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.136.500.500', 'B01.050.150.900.649.313.992.635.505.500.550.455', 'B01.050.150.900.649.313.992.635.505.500.800.500'], ['D08.811.913.400.100.200'], ['G11.561.638'], ['D12.776.157.530.400.400.500.500', 'D12.776.543.550.450.500.200.500', 'D12.776.543.585.400.500.200.500', 'D12.776.543.750.720.200.450.400.500'], ['A08.850', 'A11.284.149.165.420.780'], ['G02.111.820.850', 'G04.835.850', 'G07.265.880', 'G11.561.830']]
|
['Organisms [B]', 'Psychiatry and Psychology [F]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Impact of concentration of oesophageal and gastric cardia cancer surgery on long-term population-based survival.
|
BACKGROUND: The objective was to evaluate the impact of concentration of surgery for oesophageal and gastric cardia cancer on long-term survival in the population-based Eindhoven Cancer Registry area. In contrast to most previous studies, this study aimed to evaluate both surgically and non-surgically treated patients, to avoid the confounding effect of selective referral.METHODS: This retrospective cohort study included all patients diagnosed with oesophageal or gastric cardia cancer between 1995 and 2006. Results for the period 1995-1998 were compared with those for 1999-2006, after concentration of surgery.RESULTS: Between 1995 and 2006, 2212 patients were registered with the diagnosis, of whom 638 underwent resection. Before 1999, 73·4 per cent of surgically treated patients underwent a resection in a low-volume hospital (fewer than 4 resections per year) and 23·2 per cent were referred to an academic hospital. After concentration, 63·2 per cent of surgically treated patients underwent resection in one of two regional high-volume centres (15-20 resections per year) and 13·8 per cent were referred to an academic hospital. Three-year survival rates increased from 32·0 to 45·1 per cent for patients who had surgery (P = 0·004), and from 13·1 to 17·9 per cent for all included patients (P = 0·026). These improvements remained after adjustment for case mix or (neo)adjuvant treatments, and were similar for patients with squamous cell carcinoma or adenocarcinoma. However, adjustment for annual hospital volume attenuated this association for patients who had surgery.CONCLUSION: Concentration of oesophageal and gastric cardia cancer surgery was associated with improvements in long-term, population-based overall survival for surgically as well as non-surgically treated patients, apparently mediated by an increase in volume.
|
['Aged', 'Cardia', 'Esophageal Neoplasms', 'Female', 'Humans', 'Male', 'Middle Aged', 'Netherlands', 'Proportional Hazards Models', 'Registries', 'Retrospective Studies', 'Stomach Neoplasms', 'Survival Analysis']
| 21,509,748
|
[['M01.060.116.100'], ['A03.556.875.875.163'], ['C04.588.274.476.205', 'C04.588.443.353', 'C06.301.371.205', 'C06.405.117.430', 'C06.405.249.205'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['Z01.542.651'], ['E05.318.740.500.700', 'E05.318.740.600.700', 'E05.318.740.750.725', 'E05.318.740.998.825', 'E05.599.835.900', 'N05.715.360.750.530.650', 'N05.715.360.750.625.650', 'N05.715.360.750.695.650', 'N05.715.360.750.795.825', 'N06.850.520.830.500.700', 'N06.850.520.830.600.700', 'N06.850.520.830.750.725', 'N06.850.520.830.998.912'], ['E05.318.308.970', 'N04.452.859.819', 'N05.715.360.300.715.700', 'N06.850.520.308.970'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['C04.588.274.476.767', 'C06.301.371.767', 'C06.405.249.767', 'C06.405.748.789'], ['E05.318.740.998', 'N05.715.360.750.795', 'N06.850.520.830.998']]
|
['Named Groups [M]', 'Anatomy [A]', 'Diseases [C]', 'Organisms [B]', 'Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
[Coping with loneliness: evaluation of a group course for elderly widows].
|
The present study was designed to evaluate a group course of 11 sessions, aimed at reducing loneliness in elderly widows. Twenty widows participated (mean age: 67 yrs), who were all reasonably or very satisfied with the experience of participation as well as with the format and leadership of the course. At the beginning of the course, loneliness was a problem for each participant, and questionnaire scores for depression, well-being and social contacts (i.e. loneliness) were unfavourable. At the end of the course, one third of the participants indicated on an evaluation form that feelings of loneliness had become less intense. The corresponding questionnaire scores, however, did not improve significantly. However, rather large positive changes were found for depressive symptoms and for positive social support, which were maintained at a 3-month follow-up measurement. The participants with higher depression scores were also the ones who showed the largest gain, although their scores remained in the pathological range. It is concluded that the latter participants suffer from more serious psychological problems than is desirable in view of the aims of the course. Adaptation of the aims and contents of the course should be considered, so that individual pathology can be more adequately addressed.
|
['Adaptation, Psychological', 'Aged', 'Depression', 'Female', 'Group Processes', 'Humans', 'Loneliness', 'Middle Aged', 'Program Evaluation', 'Single Person', 'Surveys and Questionnaires']
| 7,809,918
|
[['F01.058'], ['M01.060.116.100'], ['F01.145.126.350'], ['F01.829.316'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F01.470.713', 'I01.880.853.748.435'], ['M01.060.116.630'], ['E05.337.820', 'N04.761.685', 'N05.715.360.650'], ['F01.829.263.315.500.725', 'I01.240.361.500.725', 'I01.880.853.150.423.500.725', 'M01.785', 'N01.224.361.500.725', 'N01.824.308.500.725'], ['E05.318.308.980', 'N05.715.360.300.800', 'N06.850.520.308.980']]
|
['Psychiatry and Psychology [F]', 'Named Groups [M]', 'Organisms [B]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 0
|
Concurrent primary hyperparathyroidism and humoral hypercalcemia of malignancy in a patient with clear cell endometrial cancer.
|
A 65-year-old Caucasian woman with a known history of clear cell endometrial cancer presented with hypercalcemia. Further evaluation demonstrated that the patient had primary hyperparathyroidism due to a parathyroid adenoma, as well as an increased parathyroid hormone-related peptide secondary to her malignancy. To the best of our knowledge, this is the first reported case of a female patient with concurrent primary hyperparathyroidism and humoral hypercalcemia of malignancy. This case illustrates the importance of considering a broad differential when evaluating patients with hypercalcemia. It also emphasizes the importance of recognizing the biochemical interplay between parathyroid hormone and parathyroid hormone-related peptide.
|
['Adenocarcinoma, Clear Cell', 'Adenoma', 'Aged', 'Calcium', 'Disease Progression', 'Endometrial Neoplasms', 'Female', 'Humans', 'Hypercalcemia', 'Hyperparathyroidism, Primary', 'Neoplasms, Multiple Primary', 'Parathyroid Hormone-Related Protein', 'Parathyroid Neoplasms', 'Parathyroidectomy', 'Postoperative Complications']
| 19,005,452
|
[['C04.557.470.200.025.045'], ['C04.557.470.035'], ['M01.060.116.100'], ['D01.268.552.100', 'D01.552.539.288', 'D23.119.100'], ['C23.550.291.656'], ['C04.588.945.418.948.585', 'C13.351.500.852.762.200', 'C13.351.937.418.875.200'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C18.452.174.451', 'C18.452.950.340'], ['C19.642.355.239'], ['C04.651'], ['D06.472.699.591', 'D12.644.276.908', 'D12.644.548.588', 'D12.776.467.890', 'D23.529.890'], ['C04.588.322.525', 'C04.588.443.680', 'C19.344.525', 'C19.642.713'], ['E04.270.694'], ['C23.550.767']]
|
['Diseases [C]', 'Named Groups [M]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Assessment of telomere length, phenotype, and DNA content.
|
Telomere sequences at the end of chromosomes control somatic cell division; therefore, telomere length in a given cell population provides information about its replication potential. This unit describes a method for flow cytometric measurement of telomere length in subpopulations using fluorescence in situ hybridization of fluorescently-labeled probes (Flow-FISH) without prior cell separation. After cells are stained for surface immunofluorescence, antigen-antibody complexes are covalently cross-linked onto cell membranes before FISH with a telomere-specific probe. Cells with long telomeres are included as internal standards. Addition of a DNA dye permits exclusion of proliferating cells during data analysis. DNA ploidy measurements of cells of interest and internal standard are performed on separate aliquots in parallel to Flow-FISH. Telomere fluorescence of G(0/1) cells of subpopulations and internal standards obtained from Flow-FISH are normalized for DNA ploidy and telomere length in subsets of interest is expressed as a fraction of the internal standard telomere length.
|
['Animals', 'DNA', 'Flow Cytometry', 'Humans', 'In Situ Hybridization, Fluorescence', 'Phenotype', 'Ploidies', 'Resting Phase, Cell Cycle', 'Telomere']
| 18,770,803
|
[['B01.050'], ['D13.444.308'], ['E01.370.225.500.363.342', 'E01.370.225.500.386.350', 'E05.196.712.516.600.240.350', 'E05.200.500.363.342', 'E05.200.500.386.350', 'E05.242.363.342', 'E05.242.386.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.225.500.620.670.325.350', 'E01.370.225.750.600.670.325.350', 'E05.200.500.620.670.325.350', 'E05.200.750.600.670.325.350', 'E05.393.285.350', 'E05.393.661.475.350'], ['G05.695'], ['G05.700'], ['G04.144.500.300'], ['A11.284.430.106.279.345.190.160.845', 'G05.360.160.845']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
The effect of cranberry juice and cranberry proanthocyanidins on the infectivity of human enteric viral surrogates.
|
The effect of cranberry juice (CJ) and cranberry proanthocyanidins (PAC) on the infectivity of human enteric virus surrogates, murine norovirus (MNV-1), feline calicivirus (FCV-F9), MS2(ssRNA) bacteriophage, and phiX-174(ssDNA) bacteriophage was studied. Viruses at high (approximately 7 log(10) PFU/ml) or low (approximately 5 log(10) PFU/ml) titers were mixed with equal volumes of CJ, 0.30, 0.60, and 1.20 mg/ml final PAC concentration, or water and incubated for 1 h at room temperature. Viral infectivity after treatments was evaluated using standardized plaque assays. At low viral titers, FCV-F9 was undetectable after exposure to CJ or the three tested PAC solutions. MNV-1 was reduced by 2.06 log(10) PFU/ml with CJ, and 2.63, 2.75, and 2.95 log(10) PFU/ml with 0.15, 0.30, and 0.60 mg/ml PAC, respectively. MS2 titers were reduced by 1.14 log(10) PFU/ml with CJ, and 0.55, 0.80, and 0.96 log(10) PFU/ml with 0.15, 0.30, and 0.60 mg/ml PAC, respectively. phi-X174 titers were reduced by 1.79 log(10) PFU/ml with CJ, and 1.95, 3.67, and 4.98 log(10) PFU/ml with PAC at 0.15, 0.30, and 0.60 mg/ml, respectively. Experiments using high titers showed similar trends but with decreased effects. CJ and PAC show promise as natural antivirals that could potentially be exploited for foodborne viral illness treatment and prevention.
|
['Antiviral Agents', 'Bacteriophages', 'Beverages', 'Calicivirus, Feline', 'Consumer Product Safety', 'Dose-Response Relationship, Drug', 'Foodborne Diseases', 'Humans', 'Norovirus', 'Proanthocyanidins', 'Vaccinium macrocarpon', 'Viral Load', 'Virulence']
| 20,417,404
|
[['D27.505.954.122.388'], ['B04.123'], ['G07.203.100', 'J02.200'], ['B04.820.578.298.887.150'], ['N06.850.210'], ['G07.690.773.875', 'G07.690.936.500'], ['C25.723.415'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['B04.820.578.298.550'], ['D03.383.663.283.266.450.700', 'D03.633.100.150.266.450.700', 'D05.750.078.937.429'], ['B01.650.940.800.575.912.250.341.937.774.750'], ['E01.370.225.875.950', 'E05.200.875.950', 'G06.920.850'], ['G06.930']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Technology, Industry, and Agriculture [J]', 'Health Care [N]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
|
Germline mutations of MEK in cardio-facio-cutaneous syndrome are sensitive to MEK and RAF inhibition: implications for therapeutic options.
|
Cardio-facio-cutaneous (CFC) syndrome is a sporadic developmental disorder characterized by distinctive craniofacial features, heart defects, mental retardation and ectodermal abnormalities. We recently reported missense germline mutations in the genes MEK1 and MEK2 in patients with CFC. These mutations, including F53S and Y130C MEK1, and F57C MEK2, are the first naturally occurring mutations to be identified in these genes. This study reports data concerning the biochemical functions of the novel mutants, as well as the roles of these MEK genes in the MAPK signaling cascade. Our CFC MEK variants cannot induce ERK unless they are phosphorylated by RAF at two key serine residues in the regulatory loop. When we replaced the serine residues with alanines, ERK phosphorylation was significantly reduced in the presence of RAF. We did find that F57C MEK2 activation was less dependent on RAF signaling than the other mutants. This difference results in F57C MEK2 being resistant to the selective RAF inhibitor SB-590885. All three mutants are sensitive to the MEK inhibitor U0126. The majority of CFC cases result from mutations in B-RAF. A recent report indicates the possibility that cancer cells with activated B-RAF have enhanced, selective sensitivity to MEK inhibitors. Thus, regardless of mutations identified in an individual with CFC, MEK inhibition is a potential therapeutic approach for this population.
|
['Animals', 'Binding Sites', 'COS Cells', 'Cell Line', 'Chlorocebus aethiops', 'Craniofacial Abnormalities', 'Germ-Line Mutation', 'Heart Defects, Congenital', 'Humans', 'Imidazoles', 'MAP Kinase Kinase 1', 'MAP Kinase Kinase 2', 'MAP Kinase Signaling System', 'Phosphorylation', 'Protein Kinase Inhibitors', 'Rats', 'Recombinant Proteins', 'Skin Abnormalities', 'Syndrome', 'raf Kinases']
| 17,981,815
|
[['B01.050'], ['G02.111.570.120'], ['A11.251.210.172.500', 'A11.329.228.220'], ['A11.251.210'], ['B01.050.150.900.649.313.988.400.112.199.120.126.110'], ['C05.660.207', 'C16.131.621.207'], ['G05.365.590.350'], ['C14.240.400', 'C14.280.400', 'C16.131.240.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D03.383.129.308'], ['D08.811.913.696.620.682.700.565.100', 'D08.811.913.696.620.682.725.200.100', 'D12.644.360.440.100', 'D12.776.476.440.100'], ['D08.811.913.696.620.682.700.565.200', 'D08.811.913.696.620.682.725.200.200', 'D12.644.360.440.200', 'D12.776.476.440.200'], ['G02.111.820.560', 'G03.493.560', 'G04.835.560'], ['G02.111.665', 'G02.607.780', 'G03.796'], ['D27.505.519.389.755'], ['B01.050.150.900.649.313.992.635.505.700'], ['D12.776.828'], ['C16.131.831', 'C17.800.804'], ['C23.550.288.500'], ['D08.811.913.696.620.682.700.559.842', 'D12.644.360.400.842', 'D12.776.476.400.842', 'D12.776.624.664.700.204']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Diseases [C]', 'Chemicals and Drugs [D]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Comparison of the major polypeptides of the erythrocyte nuclear envelope.
|
The three most abundant nonhistone polypeptides (molecular weights 75,000, 71,000 and 61,000) of the avian erythrocyte nucleus have previously been isolated in the nuclear envelope fraction. They have been separated by sodium dodecylsulfate-polyacrylamide gel electrophoresis and peptide-mapped after limited enzymatic digestion. Three enzymes -- chymotrypsin, papain and Staphylococcus aureus protease -- were used. Results obtained with each enzyme indicate strong similarities between the three nuclear envelope polypeptides. The amino acid compositions of the two most abundant polypeptides (P75 and P71) have been determined and found to be similar. Further, they readily yield large fragments upon brief alkaline hydrolysis. For both P75, and P71 the degree and the pattern of alkaline fragmentation are almost identical. A 61,000-dalton polypeptide which appears to be P61 is obtained from P75 and P71 by mild acid hydrolysis. These results establish the close chemical similarity of these predominant polypeptides in the erythrocyte nucleus and suggest that they serve related functions.
|
['Amino Acids', 'Animals', 'Chickens', 'Erythrocytes', 'Membrane Proteins', 'Molecular Weight', 'Nuclear Envelope', 'Nucleoproteins', 'Peptide Fragments', 'Peptides']
| 513,770
|
[]
|
[]
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Safety of transtympanic application of probiotics in a chinchilla animal model.
|
BACKGROUND: Chronic suppurative otitis media can be recalcitrant and difficult to treat, particularly with the increasing occurrence of antibiotic resistance. Lactobacillus plantarum is a probiotic that has been shown to decrease S. aureus and P. aeruginosa growth in wounds, making it a good candidate for the treatment of chronic suppurative otitis media. However, before it can be applied in the ear, its ototoxicity potential must be evaluated.METHODS: A prospective controlled trial was conducted in a chinchilla animal model at the Animal care research facilities of the Montreal Children's Hospital Research Institute to determine whether Lactobacillus plantarum is ototoxic when applied transtympanically. Ten chinchillas each had one ear randomly assigned to receive 109 CFU/mL of Lactobacillus plantarum solution, while the contralateral ear received saline. Auditory brainstem responses were measured bilaterally at 8, 20, 25 kHz before, at 7-10 days after application, and at 28 days after application of probiotic or saline. Facial nerve and vestibular function were assessed clinically.RESULTS: There were no statistically significant differences in hearing thresholds between control and experimental ears at 28 days after application. A difference of 11 dB was noted in the 25 kHz range at day 7-10, but resolved by day 28. No animals receiving probiotics developed vestibular nerve dysfunction. There was no histologic evidence of auditory hair cell damaged evidenced by scanning electron microscopy.CONCLUSION: Our study suggests that a single application of Lactobacillus plantarum at 109 CFU/mL does not cause ototoxicity in a chinchilla animal model. These preliminary safety evaluations and the pathogen inhibitory effects of L. plantarum demonstrated by previous studies present this probiotic as a candidate of interest for further investigation.
|
['Administration, Topical', 'Animals', 'Chinchilla', 'Chronic Disease', 'Disease Models, Animal', 'Dose-Response Relationship, Drug', 'Drug Administration Schedule', 'Female', 'Follow-Up Studies', 'Hearing Tests', 'Otitis Media, Suppurative', 'Probiotics', 'Random Allocation', 'Reference Values', 'Risk Assessment', 'Treatment Outcome', 'Tympanic Membrane']
| 29,166,927
|
[['E02.319.267.120'], ['B01.050'], ['B01.050.150.900.649.313.992.328'], ['C23.550.291.500'], ['C22.232', 'E05.598.500', 'E05.599.395.080'], ['G07.690.773.875', 'G07.690.936.500'], ['E02.319.283'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['E01.370.382.375'], ['C01.830.694', 'C09.218.705.663.680'], ['G07.203.300.456.500', 'J02.500.456.500'], ['E05.318.370.700', 'E05.581.500.805', 'N05.715.360.325.675', 'N06.850.520.445.700'], ['E05.978.810'], ['E05.318.740.600.800.715', 'N04.452.871.715', 'N05.715.360.750.625.700.690', 'N06.850.505.715', 'N06.850.520.830.600.800.715'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['A09.246.272.702']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Technology, Industry, and Agriculture [J]', 'Anatomy [A]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
|
Involvement of the spinal posterior horn in Gerstmann-Str?ussler-Scheinker disease (PrP P102L).
|
OBJECTIVE: The authors studied the pathomechanisms of the characteristics associated with Gerstmann-Str?ussler-Scheinker disease (GSS).BACKGROUND: GSS, associated with a missense mutation at codon 102 of the prion protein (PrP) gene (GSS102), is a hereditary disorder that presents with progressive ataxia and dementia, and is characterized by the loss of deep tendon reflexes and painful dysesthesias of the legs in its early stage.METHODS: The authors conducted immunohistochemical studies of the spinal cord and peripheral nervous system in one of two patients from a Japanese family with GSS102 in comparison with patients with GSS105.RESULTS: The authors found intense PrP immunoreactivities mainly in the posterior horn of the spinal cord, but not in the dorsal root ganglia or peripheral nerves. In addition to PrP amyloid plaques, synaptic-type, fine granular PrP deposits were distributed in the spinal posterior horns. In contrast to the GSS102 patient, the spinal cords of the GSS105 patients showed no granular PrP deposits.CONCLUSIONS: The PrP abnormalities in synaptic structures of the spinal posterior horn may cause synaptic dysfunction that leads to loss of deep tendon reflexes and painful dysesthesias in patients with GSS102.
|
['Adult', 'Codon', 'Female', 'Gerstmann-Straussler-Scheinker Disease', 'Humans', 'Immunohistochemistry', 'Mutation, Missense', 'Prions', 'Spinal Cord']
| 9,932,941
|
[['M01.060.116'], ['D13.444.735.544.355', 'G05.360.335.355', 'G05.360.340.024.340.137.190'], ['C01.207.800.350', 'C10.228.228.800.350', 'C10.574.500.425', 'C10.574.843.400', 'C16.320.400.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.225.500.607.512', 'E01.370.225.750.551.512', 'E05.200.500.607.512', 'E05.200.750.551.512', 'E05.478.583', 'H01.158.100.656.234.512', 'H01.158.201.344.512', 'H01.158.201.486.512', 'H01.181.122.573.512', 'H01.181.122.605.512'], ['G05.365.590.650'], ['D12.776.785'], ['A08.186.854']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 1
| 0
| 0
|
Morton's neuroma: a microscopic evaluation.
|
Microscopic evaluation of the interdigital neuroma is described in 24 nerves which were evaluated at six cross-section areas for size of the nerve and width of the perineural along with the fascicle diameter and the size and number of blood vessels inside each fascicle. A relationship was noted between the level of the distal edge of the intermetatarsal ligament. Cross-section that was taken at that area showed changes distally in nerve diameter, fascicle number and size, blood vessel number and size, and perineural width. These changes are consistent with the interdigital neuroma as an entrapment condition.
|
['Female', 'Foot', 'Foot Diseases', 'Humans', 'Male', 'Neuroma', 'Tarsal Joints']
| 6,519,606
|
[['A01.378.610.250'], ['C05.360', 'C17.800.321'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C04.557.580.600.610'], ['A02.835.583.378.831']]
|
['Anatomy [A]', 'Diseases [C]', 'Organisms [B]']
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
An in vitro approach to potential methadone metabolic-inhibition interactions.
|
OBJECTIVE: The aim of this study was to assess the drug interaction potential of psychotropic medication on methadone N-demethylation using cDNA-expressed cytochrome P450 CYP enzymes.METHODS: Methadone was incubated with various drugs (n = 10) and cDNA-expressed CYP3A4, CYP2D6, CYP2B6, CYP2C19 and CYP1A2 enzymes to screen for their inhibition potency. The nature of enzyme selective activity for inhibition was further investigated for potent inhibitors. To test for a mechanism-based component in inhibition, all substances were tested with preincubation and without. 2-Ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) concentration was determined by liquid chromatography/tandem mass spectrometry following liquid/liquid extraction.RESULTS: Formation of EDDP was catalysed by CYP3A4, CYP2D6 and CYP2C19. The N-demethylation of methadone was preferentially inhibited by amitriptyline, buprenorphine, methylenedioxymethamphetamine (MDMA) and zolpidem. Both amitriptyline and buprenorphine were strong, reversible inhibitors of CYP3A4. Similarly, amitriptyline and MDMA were identified as inhibitors of CYP2D6. Zolpidem revealed a mechanism-based inhibition of CYP3A4.CONCLUSION: Amitriptyline, MDMA and zolpidem are likely to slow down conversion of methadone and to increase its area under the curve (AUC). A consideration of the in vitro evidence of drug-methadone interactions should help to improve patient care during methadone maintenance treatment.
|
['Algorithms', 'Amitriptyline', 'Analgesics, Opioid', 'Atomoxetine Hydrochloride', 'Chromatography, Liquid', 'Citalopram', 'Clozapine', 'Cytochrome P-450 Enzyme Inhibitors', 'Cytochrome P-450 Enzyme System', 'Dose-Response Relationship, Drug', 'Drug Interactions', 'Isoenzymes', 'Kinetics', 'Methadone', 'Methylation', 'N-Methyl-3,4-methylenedioxyamphetamine', 'Oxidoreductases, N-Demethylating', 'Propylamines', 'Psychotropic Drugs', 'Pyridines', 'Pyrrolidines', 'Tandem Mass Spectrometry', 'Zolpidem']
| 17,598,095
|
[['G17.035', 'L01.224.050'], ['D02.455.426.559.847.181.384.100', 'D04.615.181.384.100'], ['D27.505.696.277.600.500', 'D27.505.696.663.850.014.760.500', 'D27.505.954.427.040.550.500', 'D27.505.954.427.210.600.500'], ['D02.092.831.085'], ['E05.196.181.400'], ['D02.092.831.170', 'D02.626.320', 'D03.633.100.127.187'], ['D03.633.300.240.220'], ['D27.505.389.500', 'D27.505.519.389.335'], ['D08.244.453', 'D08.811.682.690.708.170', 'D12.776.422.220.453'], ['G07.690.773.875', 'G07.690.936.500'], ['G07.690.773.968'], ['D08.811.348', 'D12.776.800.300'], ['G01.374.661', 'G02.111.490'], ['D02.522.675'], ['G02.111.035.538', 'G02.607.094.538', 'G03.059.538'], ['D02.092.471.683.152.670'], ['D08.811.682.662.582'], ['D02.092.831'], ['D27.505.954.427.700'], ['D03.383.725'], ['D03.383.773'], ['E05.196.566.880'], ['D03.383.725.971']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Psychological Factors that Lessen the Impact of COVID-19 on the Self-Employment Intention of Business Administration and Economics' Students from Latin America.
|
BACKGROUND: The 2019 coronavirus disease epidemic (Covid-19) is a public health emergency of international concern and poses a challenge to the labor market. The pandemic has a devastating and disproportionate effect on young workers, their interest in entrepreneurship, and their mental health. Research is needed to develop evidence-based strategies to improve coping and reduce adverse psychological problems. The objective of this study was to analyze the impact that Covid-19 pandemic perception and psychological need satisfaction have on university students and their self-employment intention. In addition, we also analyzed the role of moderation played by psychological aspects. These psychological factors (i.e., Optimism and Proactiveness) can also improve young people's mental health and well-being.METHODS: An explorative study (online survey) was conducted in March 2020 934 university students from Latin America. Regression analysis models were built to examine the relationships between Covid-19 pandemic perception, personality variables, and entrepreneurial intention. Mediation models, through the bootstrapping method, were performed to analyze the mediating role of proactiveness and optimism.RESULTS: Results indicate that students' perception of Covid-19 and psychological need satisfaction are associated with entrepreneurial intention. Additionally, the present study argues that proactiveness and optimism mediate these relationships.CONCLUSIONS: This study identifies psychological factors associated with a lower level of Covid-19 impact and that can be used for psychological interventions that result in an improvement in the mental health of these vulnerable groups during and after the Covid-19 pandemic. Theoretical and practical implications are discussed.
|
['Adaptation, Psychological', 'Adolescent', 'Adult', 'Betacoronavirus', 'COVID-19', 'Coronavirus Infections', 'Economics', 'Employment', 'Female', 'Humans', 'Intention', 'Latin America', 'Male', 'Mental Health', 'Pandemics', 'Pneumonia, Viral', 'SARS-CoV-2', 'Students', 'Surveys and Questionnaires', 'Young Adult']
| 32,708,034
|
[['F01.058'], ['M01.060.057'], ['M01.060.116'], ['B04.820.578.500.540.150.113'], ['C01.748.214', 'C01.748.610.763.500', 'C01.925.705.500', 'C01.925.782.600.550.200.163', 'C08.381.677.807.500', 'C08.730.214', 'C08.730.610.763.500'], ['C01.925.782.600.550.200'], ['I01.261', 'N03.219'], ['N01.824.245'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F01.658.650', 'F02.463.306'], ['Z01.107.424'], ['F02.418', 'N01.400.500'], ['N06.850.290.200.600'], ['C01.748.610.763', 'C01.925.705', 'C08.381.677.807', 'C08.730.610.763'], ['B04.820.578.500.540.150.113.968'], ['M01.848'], ['E05.318.308.980', 'N05.715.360.300.800', 'N06.850.520.308.980'], ['M01.060.116.815']]
|
['Psychiatry and Psychology [F]', 'Named Groups [M]', 'Organisms [B]', 'Diseases [C]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]', 'Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 1
|
[Cause od death statistics and death certificate documentation. 1. Historical development of cause of death statistics of fetal death, infants and children and current regulations in the GDR].
|
After having described the beginnings of documentation and statistics of causes of death of newborns and children the development of this branch of medical statistics in the GDR and the introduction of a special death certificate in 1961 is dealt with. To help obstetricians, perinatologists and pediatrists to tackle the problem of documentation of the causes of death of stillborns and newborns the 9th revision of ICD, the nowadays valid death certificate and special rules of signing are explained to avoid mistakes, which are often met with in the daily practice.
|
['Cause of Death', 'Child', 'Child, Preschool', 'Death Certificates', 'Female', 'Fetal Death', 'Germany, East', 'Humans', 'Infant', 'Infant Mortality', 'Infant, Newborn', 'Mortality', 'Pregnancy', 'Vital Statistics']
| 2,796,126
|
[['E05.318.308.985.550.250', 'N01.224.935.698.100', 'N06.850.505.400.975.550.250', 'N06.850.520.308.985.550.250'], ['M01.060.406'], ['M01.060.406.448'], ['E05.318.308.940.350', 'L01.399.250.900.350', 'N04.452.859.264', 'N05.715.360.300.715.315', 'N06.850.520.308.940.350'], ['C13.703.223', 'C23.550.260.585'], ['Z01.586.338'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703'], ['E05.318.308.985.550.475', 'N01.224.935.698.489', 'N06.850.505.400.975.550.475', 'N06.850.520.308.985.550.475'], ['M01.060.703.520'], ['E05.318.308.985.550', 'N01.224.935.698', 'N06.850.505.400.975.550', 'N06.850.520.308.985.550'], ['G08.686.784.769'], ['E05.318.308.985', 'N01.224.935', 'N06.850.505.400.975', 'N06.850.520.308.985']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Named Groups [M]', 'Information Science [L]', 'Diseases [C]', 'Geographicals [Z]', 'Organisms [B]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 1
| 1
| 1
|
Serum thyroxine concentrations following fixed-dose radioactive iodine treatment in hyperthyroid cats: 62 cases (1986-1989).
|
The medical records of 62 hyperthyroid cats treated with a fixed dose of 4 mCi of radioactive iodine (131I) were reviewed. In 60 cats, serum thyroxine concentrations were determined after treatment, allowing evaluation of treatment success. Eighty-four percent of the cats had normal serum thyroxine concentrations after treatment. Five of the 60 cats (8%) remained hyperthyroxinemic after treatment. Five cats (8%) were hypothyroxinemic when evaluated within 60 days of treatment. Three of these cats had normal serum thyroxine concentrations 6 months after treatment, and none had clinical signs of hypothyroidism. The administration of a fixed dose of 4 mCi of 131I was determined to be an effective treatment for feline hyperthyroidism.
|
['Animals', 'Cat Diseases', 'Cats', 'Female', 'Hyperthyroidism', 'Iodine Radioisotopes', 'Male', 'Retrospective Studies', 'Thyroxine']
| 2,211,313
|
[['B01.050'], ['C22.180'], ['B01.050.150.900.649.313.750.377.750.250.125'], ['C19.874.397'], ['D01.268.380.400.500.496', 'D01.496.448.496', 'D01.496.749.474'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['D06.472.931.812', 'D12.125.072.050.767']]
|
['Organisms [B]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Successful snakebite treatment in three juvenile African wild dogs (Lycaon pictus) with polyvalent antivenom: a Namibian case report.
|
This article reports the first documented treatment of venomous snakebite with a polyvalent snake antivenom from the South African Institute for Medical Research in endangered African wild dogs (Lycaon pictus). Three juvenile male animals (6.5 months of age) showed clinical signs after being bitten by an unidentified venomous snake. The signs included loss of appetite, disorientation, impaired locomotion, excessive facial swelling, profuse salivation, reduced respiratory effort and an apparent depressed mental state. Intravenous treatment with isotonic Ringer lactate solution, hetastarch 6% and dexamethazone, subcutaneous administration of procaine benzylpenicillin and benzathine benzylpenicillin, and ultimately intravenous administration of the polyvalent snake antivenom resulted in the complete recovery of all three wild dogs.
|
['Anesthesia, General', 'Animals', 'Antivenins', 'Behavior, Animal', 'Canidae', 'Fluid Therapy', 'Male', 'Namibia', 'Snake Bites', 'Social Behavior']
| 23,718,740
|
[['E03.155.197'], ['B01.050'], ['D12.776.124.486.485.114.573.601.138', 'D12.776.124.790.651.114.573.601.138', 'D12.776.377.715.548.114.573.601.138', 'D20.215.401.601.163'], ['F01.145.113'], ['B01.050.150.900.649.313.750.250.216'], ['E02.319.360'], ['Z01.058.290.175.580'], ['C25.723.127.442', 'C26.176.724'], ['F01.145.813']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Psychiatry and Psychology [F]', 'Geographicals [Z]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
|
An efficient and reproducible protocol for the production of salt tolerant transgenic wheat plants expressing the Arabidopsis AtNHX1 gene.
|
We present an efficient method for the production of transgenic salt tolerant hexaploid wheat plants expressing the Arabidopsis AtNHX1 gene. Wheat mature zygotic embryos were isolated from two hexaploid bread wheat (Triticum aestivum) cultivars (namely: Gemmeiza 9 and Gemmeiza 10) and were transformed with the A. tumefaciens LBA4404 harboring the pBI-121 vector containing the AtNHX1 gene. Transgenic wheat lines that express the gus intron was obtained and used as control. The results confirmed that npt-II gene could be transmitted and expressed in the T2 following 3:1 Mendelian segregation while the control plant couldn't. The data indicate that, the AtNHX1 gene was integrated in a stable manner into the wheat genome and the corresponding transcripts were expressed. The transformation efficiency was 5.7 and 7.5% for cultivars Gemmeiza 10 and Gemmeiza 9, respectively. A greenhouse experiment was conducted to investigate the effect of AtNHX1 gene in wheat salt tolerance. The transgenic wheat lines could maintain high growth rate under salt stress condition (350 mM NaCl) while the control plant couldn't. The results confirmed that Na(+)/H(+) antiporter gene AtNHX1 increased salt tolerance by increasing Na(+) accumulation and keeping K+/Na(+) balance. Thus, transgenic plants showed high tolerance to salt stress and can be considered as a new genetic resource in breeding programs.
|
['Arabidopsis', 'Arabidopsis Proteins', 'Breeding', 'Cation Transport Proteins', 'Gene Transfer Techniques', 'Genome, Plant', 'Plants, Genetically Modified', 'Salt-Tolerant Plants', 'Sodium-Hydrogen Exchangers', 'Transformation, Genetic', 'Triticum']
| 25,007,249
|
[['B01.650.940.800.575.912.250.157.100'], ['D12.776.765.149'], ['E05.820.150', 'G05.090'], ['D12.776.157.530.450.250', 'D12.776.543.585.450.250'], ['E05.393.350'], ['G05.360.340.365'], ['B01.650.520', 'B05.620.600'], ['B01.650.723'], ['D12.776.157.530.450.162.775', 'D12.776.157.530.937.703', 'D12.776.543.550.190.775', 'D12.776.543.585.450.162.775', 'D12.776.543.585.937.828'], ['G05.728.865'], ['B01.650.940.800.575.912.250.822.918']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.