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The protection of resveratrol and its combination with glibenclamide, but not berberine on the diabetic hearts against reperfusion-induced arrhythmias: the role of myocardial KATP
|
CONTEXT: Cardiovascular dysfunctions such as life-threatening arrhythmias are one of the main reasons of mortality and morbidity in diabetic patients Objective: We aimed to investigate the long-term effects of resveratrol, berberine and glibenclamide combinations on the ischemia/reperfusion (I/R) induced arrhythmias in streptozotocin (STZ)-induced diabetic rats and to investigate the role of myocardial KATP channel in the possible anti-arrhythmic actions of the treatments.METHODS: Two days after induction of diabetes, diabetic rats were treated with resveratrol [5 mg/kg, intraperitoneally (i.p.)], berberine (10 mg/kg, i.p) and glibenclamide (5 mg/kg, i.p) for 6 weeks. On the 43th day, experimental animals were subjected to 6-min ischemia and 6-min reperfusion in vivo.RESULTS: The protein expression of Kir6.2 subunits was downregulated in the diabetic hearts. However, all drug treatments restored the protein expression of Kir6.2 subunits. Resveratrol alone and its combination with glibenclamide decreased the arrhythmia score, the arrhythmic period and the incidence of other types of arrhythmias during the reperfusion period.CONCLUSIONS: The combination of resveratrol with glibenclamide may alleviate reperfusion-induced arrhythmias via an underlying mechanism not be only associated with the restoration of the protein expression of Kir6.2 subunits but also associated with the other subunits or ion channels underlying cardiac action potential.
|
['Animals', 'Arrhythmias, Cardiac', 'Berberine', 'Diabetes Mellitus, Experimental', 'Dose-Response Relationship, Drug', 'Drug Interactions', 'Glyburide', 'Heart', 'Hemodynamics', 'Male', 'Myocardial Reperfusion Injury', 'Potassium Channels, Inwardly Rectifying', 'Rats', 'Rats, Sprague-Dawley', 'Resveratrol']
| 29,457,517
|
[['B01.050'], ['C14.280.067', 'C23.550.073'], ['D03.132.098.057.100', 'D03.633.400.168.100'], ['C18.452.394.750.074', 'C19.246.240', 'E05.598.500.374'], ['G07.690.773.875', 'G07.690.936.500'], ['G07.690.773.968'], ['D02.065.950.828.575', 'D02.886.590.795.575'], ['A07.541'], ['G09.330.380'], ['C14.280.238.615', 'C14.280.647.625', 'C14.907.585.625', 'C14.907.725.600', 'C23.550.767.877.500'], ['D12.776.157.530.400.600.450', 'D12.776.543.550.450.750.450', 'D12.776.543.585.400.750.450'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.750'], ['D02.455.426.559.389.150.700.725.875', 'D02.455.426.559.389.657.715.500']]
|
['Organisms [B]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Hearing loss with cochlear modiolar defects and large vestibular aqueducts.
|
OBJECTIVE: Defects of the cochlear modiolus have been found to be associated with most cases of large vestibular aqueduct. The clinical significance of these modiolar defects has not been studied previously. The purpose of this article is to correlate clinical (functional) parameters, such as hearing outcomes, with the severity of the radiographic findings in these dysplastic inner ears.STUDY DESIGN: The study design was a retrospective chart review, supplemented with telephone interviews and clinic visits.SETTING: The study was conducted at an academic, tertiary care center.PATIENTS: Thirty consecutive patients with large vestibular aqueducts participated.RESULTS: Scores of modiolar deficiencies yielded inconsistent correlations with hearing loss. Vestibular aqueduct morphology and thickness correlated very strongly with the severity of hearing loss.CONCLUSIONS: These observations support the hypothesis that large vestibular aqueduct-related hearing loss may be caused by transmission of subarachnoid pressure forces into the inner ear. However, the thickness and morphology of the vestibular aqueduct may simply be markers for more subtle cochlear dysplasia manifest by modiolar deficiency.
|
['Adolescent', 'Adult', 'Auditory Threshold', 'Child', 'Child, Preschool', 'Cochlea', 'Cognition Disorders', 'Disease Progression', 'Female', 'Follow-Up Studies', 'Hearing Loss, Conductive', 'Humans', 'Infant', 'Male', 'Middle Aged', 'Retrospective Studies', 'Speech Reception Threshold Test', 'Vestibular Aqueduct']
| 9,596,180
|
[['M01.060.057'], ['M01.060.116'], ['F02.463.593.071.173', 'F02.463.593.710.190', 'G07.888.125.173'], ['M01.060.406'], ['M01.060.406.448'], ['A09.246.300.246'], ['F03.615.250'], ['C23.550.291.656'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['C09.218.458.341.562', 'C10.597.751.418.341.562', 'C23.888.592.763.393.341.562'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703'], ['M01.060.116.630'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E01.370.382.375.060.060.760'], ['A09.246.300.909.957']]
|
['Named Groups [M]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]']
| 1
| 1
| 1
| 0
| 1
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| 1
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|
Predicting Depression among Patients with Diabetes Using Longitudinal Data. A Multilevel Regression Model.
|
INTRODUCTION: This article is part of the Focus Theme of Methods of Information in Medicine on "Big Data and Analytics in Healthcare".BACKGROUND: Depression is a common and often undiagnosed condition for patients with diabetes. It is also a condition that significantly impacts healthcare outcomes, use, and cost as well as elevating suicide risk. Therefore, a model to predict depression among diabetes patients is a promising and valuable tool for providers to proactively assess depressive symptoms and identify those with depression.OBJECTIVES: This study seeks to develop a generalized multilevel regression model, using a longitudinal data set from a recent large-scale clinical trial, to predict depression severity and presence of major depression among patients with diabetes.METHODS: Severity of depression was measured by the Patient Health Questionnaire PHQ-9 score. Predictors were selected from 29 candidate factors to develop a 2-level Poisson regression model that can make population-average predictions for all patients and subject-specific predictions for individual patients with historical records. Newly obtained patient records can be incorporated with historical records to update the prediction model. Root-mean-square errors (RMSE) were used to evaluate predictive accuracy of PHQ-9 scores. The study also evaluated the classification ability of using the predicted PHQ-9 scores to classify patients as having major depression.RESULTS: Two time-invariant and 10 time-varying predictors were selected for the model. Incorporating historical records and using them to update the model may improve both predictive accuracy of PHQ-9 scores and classification ability of the predicted scores. Subject-specific predictions (for individual patients with historical records) achieved RMSE about 4 and areas under the receiver operating characteristic (ROC) curve about 0.9 and are better than population-average predictions.CONCLUSIONS: The study developed a generalized multilevel regression model to predict depression and demonstrated that using generalized multilevel regression based on longitudinal patient records can achieve high predictive ability.
|
['California', 'Causality', 'Computer Simulation', 'Decision Support Systems, Clinical', 'Depression', 'Diabetes Complications', 'Electronic Health Records', 'Humans', 'Longitudinal Studies', 'Machine Learning', 'Natural Language Processing', 'Prevalence', 'Prognosis', 'Proportional Hazards Models', 'Regression Analysis', 'Reproducibility of Results', 'Risk Factors', 'Sensitivity and Specificity']
| 26,577,265
|
[['Z01.107.567.875.580.200', 'Z01.107.567.875.760.200'], ['N05.715.350.200', 'N06.850.490.625'], ['L01.224.160'], ['L01.313.500.750.300.190'], ['F01.145.126.350'], ['C19.246.099'], ['E05.318.308.940.968.625.500', 'N04.452.859.564.650.125', 'N05.715.360.300.715.500.530.250', 'N06.850.520.308.940.968.625.250'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.372.500.750.500', 'N05.715.360.330.500.750.500', 'N06.850.520.450.500.750.500'], ['G17.035.250.500', 'L01.224.050.375.530'], ['L01.224.050.375.580'], ['E05.318.308.985.525.750', 'N01.224.935.597.750', 'N06.850.505.400.975.525.750', 'N06.850.520.308.985.525.750'], ['E01.789'], ['E05.318.740.500.700', 'E05.318.740.600.700', 'E05.318.740.750.725', 'E05.318.740.998.825', 'E05.599.835.900', 'N05.715.360.750.530.650', 'N05.715.360.750.625.650', 'N05.715.360.750.695.650', 'N05.715.360.750.795.825', 'N06.850.520.830.500.700', 'N06.850.520.830.600.700', 'N06.850.520.830.750.725', 'N06.850.520.830.998.912'], ['E05.318.740.750', 'N05.715.360.750.695', 'N06.850.520.830.750'], ['E05.318.370.725', 'E05.337.851', 'N05.715.360.325.685', 'N06.850.520.445.725'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['E05.318.370.800', 'E05.318.740.872', 'G17.800', 'N05.715.360.325.700', 'N05.715.360.750.725', 'N06.850.520.445.800', 'N06.850.520.830.872']]
|
['Geographicals [Z]', 'Health Care [N]', 'Information Science [L]', 'Psychiatry and Psychology [F]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 0
| 1
| 1
| 1
| 0
| 0
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| 1
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| 1
| 1
|
Further characterization of the interaction between L-selectin and its endothelial ligands.
|
L-Selectin is a lectin-like receptor on lymphocytes which mediates their attachment to high endothelial venules (HEV) within lymph nodes. Previous work has identified HEV-associated endothelial ligands for L-selectin as sialylated, fucosylated and sulphated glycoproteins of approximately 50 kDa and approximately 90 kDa (Sgp50 and Sgp90). The interaction of L-selectin with these ligands is carbohydrate directed, reflecting the involvement of its amino-terminal, calcium-type lectin domain. It has been reported, and we have confirmed, that anti-Ly22 blocks the adhesive function of L-selectin without reducing its binding to a carbohydrate- based ligand PPME (phosphomannan monoester core from Hansenula hostii). The epitope for this monoclonal antibody depends on the epidermal growth factor (EGF) domain of L-selectin. We demonstrate that anti-Ly22 inhibits the interaction of L-selectin with both of the Sgps, thus establishing that the interaction of L-selectin with HEV can be accounted for by the Sgps. Furthermore, the interaction of trypsin fragments of Sgp50 with L-selectin is inhibitable both by an antibody that maps to the lectin domain and by anti-Ly22. These findings raise the possibility that anti-Ly22 is affecting the function of the lectin domain of L-selectin rather than directly antagonizing the EGF domain. Toward a further characterization of L-selectin's carbohydrate specificity, we show that Sgp50 is partially inactivated by the linkage-specific Newcastle Disease virus sialidase (alpha 2,3 linkage). We additionally demonstrate that a sialyl Lewis x-related tetrasaccharide can interact with L-selectin, as has also been demonstrated for E-selectin and P-selectin.
|
['Animals', 'Antibodies, Monoclonal', 'Binding Sites', 'Cell Adhesion Molecules', 'Chemical Precipitation', 'Clusterin', 'Endothelium, Vascular', 'Epidermal Growth Factor', 'Glycoproteins', 'L-Selectin', 'Lewis X Antigen', 'Mannans', 'Mice', 'Molecular Chaperones', 'N-Acetylneuraminic Acid', 'Neuraminidase', 'Newcastle disease virus', 'Oligosaccharides', 'Peptide Fragments', 'Rats', 'Sialic Acids', 'Trypsin']
| 1,384,820
|
[['B01.050'], ['D12.776.124.486.485.114.224', 'D12.776.124.790.651.114.224', 'D12.776.377.715.548.114.224'], ['G02.111.570.120'], ['D12.776.395.550.200', 'D12.776.543.550.200', 'D23.050.301.350'], ['E05.196.150', 'G02.159'], ['D12.776.395.207', 'D12.776.580.215'], ['A07.015.700.500', 'A10.272.491.355'], ['D06.472.317.350', 'D12.644.276.382.500', 'D12.776.467.382.500', 'D23.529.382.500'], ['D09.400.430', 'D12.776.395'], ['D12.776.395.550.200.625.903', 'D12.776.395.550.200.700.510', 'D12.776.503.843.510', 'D12.776.543.550.200.625.903', 'D12.776.543.550.200.700.510', 'D12.776.543.750.705.877.903', 'D23.050.301.350.625.903', 'D23.050.301.350.700.510'], ['D23.050.285.050.050', 'D23.050.301.264.900.050', 'D23.050.301.264.965.500', 'D23.050.301.290.544.059', 'D23.050.550.325.050', 'D23.050.705.230.544.059', 'D23.101.100.900.050', 'D23.101.140.075.050'], ['D09.698.550'], ['B01.050.150.900.649.313.992.635.505.500'], ['D12.776.580'], ['D02.241.081.844.562.668.050', 'D02.241.511.902.562.668.050', 'D09.067.687.668.030', 'D09.811.589.668.030'], ['D08.811.277.450.692'], ['B04.820.480.937.600.650.070.600'], ['D09.698.629'], ['D12.644.541'], ['B01.050.150.900.649.313.992.635.505.700'], ['D02.241.081.844.562.668', 'D02.241.511.902.562.668', 'D09.067.687.668', 'D09.811.589.668'], ['D08.811.277.656.300.760.895', 'D08.811.277.656.959.350.895']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Institutions of the European Union: medical perspective.
|
The way through the institutions of the European Union (EU) is very difficult. The difficulties will increase with the expansion of EU to 25 member states, and even more so in the area of medical societies. Following the agenda of the EU, an efficient public health service is a responsibility of every member country. The possibilities of medical interest are pointed out in this paper
|
['Europe', 'European Union', 'Humans', 'International Cooperation', 'Ophthalmology', 'Public Health Administration']
| 16,193,665
|
[['Z01.542'], ['I01.615.500.475'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['I01.615.500'], ['H02.403.810.468'], ['N04.452.794']]
|
['Geographicals [Z]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Organisms [B]', 'Disciplines and Occupations [H]', 'Health Care [N]']
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 1
| 1
|
A Phase II trial of docetaxel and cisplatin in patients with recurrent or metastatic nasopharyngeal carcinoma.
|
UNLABELLED: A Phase II study was conducted to determine the efficacy and toxicity of the combination of docetaxel and cisplatin, in patients with recurrent or metastatic nasopharyngeal carcinoma (NPC). Nine patients (median age 39 years) with NPC were enrolled, none had prior chemotherapy for their recurrent or metastatic disease. Docetaxel was administered as a 1-h intravenous infusion at a dose of 75 mg/m(2) on day 1; cisplatin was administered at a dose of 75 mg/m(2) on day 1, immediately after docetaxel. Treatment was repeated every 3 weeks. The primary endpoint was objective response rate and the secondary endpoints were duration of response, time to progression, and overall survival. A total of 45 chemotherapy cycles were administered. In an intention-to-treat analysis two patients (22%, 95% confidence interval (CI): 3-60%) achieved a partial response. The median duration of response was 4.1 months, the median time to progression 8.4 months and the overall survival at 1 year from start of treatment was 76%. Grade 3-4 neutropenia was observed in all (100%) patients over 93% of the treatment cycles, and in three cases this was complicated by fever. Other toxicities were mild.CONCLUSIONS: The combination of docetaxel and cisplatin has manageable toxicity but little efficacy as first-line treatment in patients with recurrent or metastatic NPC. In view of the low response rate, accrual was terminated and the trial was aborted.
|
['Adult', 'Antineoplastic Combined Chemotherapy Protocols', 'Cisplatin', 'Docetaxel', 'Female', 'Humans', 'Male', 'Middle Aged', 'Nasopharyngeal Neoplasms', 'Neoplasm Recurrence, Local', 'Neutropenia', 'Paclitaxel', 'Taxoids', 'Treatment Outcome']
| 12,167,421
|
[['M01.060.116'], ['E02.183.750.500', 'E02.319.077.500', 'E02.319.310.037'], ['D01.210.375', 'D01.625.125', 'D01.710.100'], ['D02.455.426.392.368.242.888.389', 'D02.455.849.291.850.389'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['C04.588.443.665.710.650', 'C07.550.350.650', 'C07.550.745.650', 'C09.647.710.650', 'C09.775.350.650', 'C09.775.549.650'], ['C04.697.655', 'C23.550.727.655'], ['C15.378.553.546.184.564'], ['D02.455.426.392.368.242.888.777', 'D02.455.849.291.850.777'], ['D02.455.426.392.368.242.888', 'D02.455.849.291.850'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Diseases [C]', 'Health Care [N]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
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|
[Extravesical antirefluxplasty (author's transl)].
|
The indications and the detailed technique of extravesical antirefluxplasty are presented. Realized on 409 ureters (247 cases), extravesical antirefluxplasty has been successful in 95% of these cases. The failures, i.e., obstructions (1,5%) and persistent reflux (3%), are analized.
|
['Humans', 'Methods', 'Suture Techniques', 'Ureter', 'Vesico-Ureteral Reflux']
| 327,648
|
[['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.581'], ['E04.987.775'], ['A05.810.776'], ['C12.777.829.920', 'C13.351.968.829.920']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Diseases [C]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Ultrasound-Guided Diagnosis of Femoral Osteomyelitis and Abscess.
|
Skin and soft tissue infections are common disease presentations to the pediatric emergency department, and rapid and accurate identification of potentially serious skin and soft tissue infections is critical. In cases of atraumatic musculoskeletal pain with systemic complaints, a bacterial etiology must be ruled out. Point-of-care ultrasonography is increasingly common in the pediatric emergency department and assists in rapid and accurate identification of a variety of disease processes. We present a case of a 14-year-old adolescent boy with atraumatic right knee pain to illustrate the benefits of point-of-care ultrasonography in the timely diagnosis of musculoskeletal and soft tissue pathology. Moreover, we describe the use of ultrasound in procedural guidance of deep-space fluid aspiration, with an eventual diagnosis of femoral osteomyelitis. Ultrasonographic techniques and the emergent work-up and management of osteomyelitis are reviewed.
|
['Abscess', 'Adolescent', 'Humans', 'Magnetic Resonance Imaging', 'Male', 'Osteomyelitis', 'Point-of-Care Systems', 'Staphylococcus aureus', 'Ultrasonography, Interventional']
| 26,335,234
|
[['C01.830.025', 'C23.550.470.756.100'], ['M01.060.057'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.350.825.500'], ['C01.160.495', 'C05.116.165.495'], ['N04.452.442.452.452.680', 'N04.452.515.360.652', 'N04.590.874'], ['B03.300.390.400.800.750.100', 'B03.353.500.750.750.100', 'B03.510.100.750.750.100', 'B03.510.400.790.750.100'], ['E01.370.350.850.855', 'E04.502.890']]
|
['Diseases [C]', 'Named Groups [M]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
[Prognostic evaluation of unfavorable coronary atherosclerosis].
|
The aim of the study was optimization of the evaluation of questioning data for detection of prognostically unfavorable coronary atherosclerosis (PUCA). The subjects were 124 patients (96 men; 28 women) with chest pain and coronary atherosclerosis risk factors. The patients were questioned in detail; the character of pain syndrome was analyzed; a priori probability (APP) of coronary heart disease was evaluated; a functional class according to Canadian Cardiovascular Society Functional Classification was established. All the patients underwent selective coronaroangiography (CAG). Coronary atherosclerosis was considered to be severe (PUCA) under the following conditions: 1) stenosis of the main trunk of the left coronary artery by at least 70% of the diameter; 2) stenosis of the anterior descending artery in the first segment by at least 95% of the diameter in the presence of stenosis of the other magistral coronary artery by at least 75% of the diameter; 3) stenosis of all the three magistral coronary arteries by at least 75% of the diameter. After the matching of the questioning data with CAG results, a chart of preliminary evaluation of APP of severe coronary atherosclerosis, approachable for general practitioners, was developed. The offered chart of evaluation of PUCA APP is characterized by high rate of reliable diagnostic conclusions (56.5 +/- 4.5%), and allows substantial decrease in the need for loading tests when selection for CAG is performed.
|
['Adult', 'Aged', 'Angina Pectoris', 'Coronary Angiography', 'Coronary Artery Bypass', 'Coronary Artery Disease', 'Female', 'Humans', 'Male', 'Middle Aged', 'Patient Selection', 'Prognosis', 'Risk Factors', 'Severity of Illness Index', 'Surveys and Questionnaires']
| 16,502,719
|
[['M01.060.116'], ['M01.060.116.100'], ['C14.280.647.187', 'C14.907.585.187', 'C23.888.592.612.233.500'], ['E01.370.350.130.625', 'E01.370.350.700.060.200', 'E01.370.370.050.200', 'E01.370.370.380.200'], ['E04.100.376.719.332', 'E04.100.814.868.750', 'E04.928.220.520.220'], ['C14.280.647.250.260', 'C14.907.137.126.339', 'C14.907.585.250.260'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['E05.581.500.653', 'N04.590.731'], ['E01.789'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['E05.318.308.980.438.475.456.500', 'N05.715.360.300.800.438.375.364.500', 'N06.850.520.308.980.438.475.364.500'], ['E05.318.308.980', 'N05.715.360.300.800', 'N06.850.520.308.980']]
|
['Named Groups [M]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Health Care [N]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
[Rooming-in care of low birth weight infants].
|
Two groups were compared: 50 small infants were together with their mothers upon their wish, 50 were in traditional separated units, because mothers refused rooming-in care. More primiparas and non smoking women chose the rooming-in system. In this group all of them breast fed their babies at discharge from hospital. In the traditional group one baby left for adoption, another three mothers went home before the discharge of their infants. In this group significantly fewer newborns were exclusively breast fed. The rooming-in system was safe for low birth-weight babies.
|
['Breast Feeding', 'Humans', 'Hungary', 'Infant Care', 'Infant, Low Birth Weight', 'Infant, Newborn', 'Infant, Premature', 'Infant, Small for Gestational Age', 'Rooming-in Care']
| 2,345,646
|
[['F01.145.407.199', 'G07.203.650.195', 'G07.203.650.220.500.500', 'G07.203.650.353.199'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['Z01.542.248.495'], ['N02.421.088.120'], ['M01.060.703.520.460'], ['M01.060.703.520'], ['M01.060.703.520.520'], ['M01.060.703.520.460.560'], ['N02.421.088.120.240']]
|
['Psychiatry and Psychology [F]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Geographicals [Z]', 'Health Care [N]', 'Named Groups [M]']
| 0
| 1
| 0
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Increased Left Atrial Appendage Density on Computerized Tomography is Associated with Cardioembolic Stroke.
|
BACKGROUND AND PURPOSE: While studies have stratified cardioembolic (CE) stroke risk by qualitative left atrial appendage (LAA) morphology and biomarkers of atrial dysfunction, the quantitative properties that underlie these observations are not well established. Accordingly, we hypothesized that LAA volume and contrast density (attenuation) on computerized tomography (CT) may capture the structural and hemodynamic processes that underlie CE stroke risk.METHODS: Data were collected from a single center prospective ischemic stroke database over 18 months and included all patients with ischemic stroke who previously underwent routine, nongated, contrast enhanced thin-slice (?2.5 mm) chest CT. Stroke subtype was determined based on the inpatient diagnostic evaluation. LAA volume and attenuation were determined from CT studies performed for various clinically appropriate indications. Univariate and multivariable analyses were performed to determine factors associated with ischemic stroke subtype, including known risk factors and biomarkers, as well as LAA density and morphologic measures.RESULTS: We identified 311 patients with a qualifying chest CT (119 CE subtype, 109 Embolic Stroke of Undetermined Source (ESUS), and 83 non-CE). In unadjusted models, there was an association between CE (versus non-CE) stroke subtype and LAA volume (OR per mL increase 1.15, 95% CI 1.07-1.24, P < .001) and LAA density (4th quartile versus 1st quartile; OR 2.95, 95% CI 1.28-6.80, P = .011), but not with ESUS (versus non-CE) subtype. In adjusted models, only the association between LAA density and CE stroke subtype persisted (adjusted OR 3.71, 95% CI 1.37-10.08, P = .010).CONCLUSION: The LAA volume and density values on chest CT are associated with CE stroke subtype but not ESUS subtype. Patients with ESUS and increased LAA volume or attenuation may be a subgroup where the mechanism is CE and anticoagulation can be tested for secondary stroke prevention.
|
['Aged', 'Aged, 80 and over', 'Atrial Appendage', 'Databases, Factual', 'Embolism', 'Female', 'Heart Diseases', 'Hemodynamics', 'Humans', 'Male', 'Middle Aged', 'Predictive Value of Tests', 'Prognosis', 'Prospective Studies', 'Risk Assessment', 'Risk Factors', 'Stroke', 'Tomography, X-Ray Computed']
| 31,932,211
|
[['M01.060.116.100'], ['M01.060.116.100.080'], ['A07.541.358.100'], ['L01.313.500.750.300.188.400', 'L01.470.750.750'], ['C14.907.355.350'], ['C14.280'], ['G09.330.380'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['E05.318.370.800.650', 'N05.715.360.325.700.640', 'N06.850.520.445.800.650'], ['E01.789'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['E05.318.740.600.800.715', 'N04.452.871.715', 'N05.715.360.750.625.700.690', 'N06.850.505.715', 'N06.850.520.830.600.800.715'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['C10.228.140.300.775', 'C14.907.253.855'], ['E01.370.350.350.810', 'E01.370.350.600.350.700.810', 'E01.370.350.700.700.810', 'E01.370.350.700.810.810', 'E01.370.350.825.810.810']]
|
['Named Groups [M]', 'Anatomy [A]', 'Information Science [L]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 1
| 1
| 0
|
Home accidents to children under 15 years: survey of 910 cases.
|
Out of 910 accidents sustained by children under 15 seen at the casualty department of a local hospital 678 (74.5%) were to children under 5 years of age. Boys were more prone to accidents than girls, and in preschool children the highest incidence of accidents was among the 2-to 3-year-olds of both sexes. Social class had no significant bearing on the accident rate. The fact that the average size of families with children under 5 was higher among families living in council houses than among those living in private houses appeared to have some bearing on the higher incidence of accidents among children under 5 living in council houses. There appeared to be no peak month when accidents were more frequent and the incidence of accidents was not significantly high on any particular weekday. In 95% of the cases one or both parents were in charge of the child at the time of the accident.Cuts were the most common types of accident followed by falls and poisoning. Among other accidents crushed fingers were as frequent as burns. A total of 62 patients (6.8% of all cases) were admitted as inpatients. Of the actual causes of the cuts and falls playing, fighting, and misbehaving were the most common followed by falling from beds, chairs, etc. While there is a need for health education programmes to draw attention to the specific dangers evidenced there clearly will always be home accidents.
|
['Accident Prevention', 'Accidents, Home', 'Adolescent', 'Burns', 'Child', 'Child, Preschool', 'England', 'Female', 'Humans', 'Infant', 'Infant, Newborn', 'Male', 'Poisoning', 'Sex Factors', 'Social Environment', 'Wounds and Injuries']
| 4,852,285
|
[['N06.850.135.060'], ['N06.850.135.217'], ['M01.060.057'], ['C26.200'], ['M01.060.406'], ['M01.060.406.448'], ['Z01.542.363.300'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703'], ['M01.060.703.520'], ['C25.723'], ['N05.715.350.675', 'N06.850.490.875'], ['I01.880.853.500'], ['C26']]
|
['Health Care [N]', 'Named Groups [M]', 'Diseases [C]', 'Geographicals [Z]', 'Organisms [B]', 'Anthropology, Education, Sociology, and Social Phenomena [I]']
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 1
|
Insulin stimulates glucose metabolism via the pentose phosphate pathway in Drosophila Kc cells.
|
Drosophila melanogaster has become a prominent and convenient model for analysis of insulin action. However, to date very little is known regarding the effect of insulin on glucose uptake and metabolism in Drosophila. Here we show that, in contrast to effects seen in mammals, insulin did not alter [(3)H]2-deoxyglucose uptake and in fact decreased glycogen synthesis ( approximately 30%) in embryonic Drosophila Kc cells. Insulin significantly increased ( approximately 1.5-fold) the production of (14)CO(2) from D-[1-(14)C]glucose while the production of (14)CO(2) from D-[6-(14)C]glucose was not altered. Thus, insulin-stimulated glucose oxidation did not occur via increasing Krebs cycle activity but rather by stimulating the pentose phosphate pathway. Indeed, inhibition of the oxidative pentose phosphate pathway by 6-aminonicotinamide abolished the effect of insulin on (14)CO(2) from D-[U-(14)C]glucose. A corresponding increase in lactate production but no change in incorporation of D-[U-(14)C]glucose into total lipids was observed in response to insulin. Glucose metabolism via the pentose phosphate pathway may provide an important source of 5'-phosphate for DNA synthesis and cell replication. This novel observation correlates well with the fact that control of growth and development is the major role of insulin-like peptides in Drosophila. Thus, although intracellular signaling is well conserved, the metabolic effects of insulin are dramatically different between Drosophila and mammals.
|
['6-Aminonicotinamide', 'Animals', 'Carbon Dioxide', 'Carbon Radioisotopes', 'Cell Line', 'Deoxyglucose', 'Drosophila melanogaster', 'Glucose', 'Glycogen', 'Insulin', 'Lactic Acid', 'Pentose Phosphate Pathway', 'Tritium']
| 14,644,433
|
[['D03.066.515.530.248', 'D03.383.725.547.530.248'], ['B01.050'], ['D01.200.200', 'D01.362.150', 'D01.650.550.200'], ['D01.268.150.075.328', 'D01.496.123.328', 'D01.496.749.154'], ['A11.251.210'], ['D09.254.229'], ['B01.050.500.131.617.720.500.500.750.310.250.500'], ['D09.947.875.359.448'], ['D05.750.078.562.388', 'D09.698.365.388'], ['D06.472.699.587.200.500.625', 'D12.644.548.586.200.500.625'], ['D02.241.511.459.450'], ['G02.111.158.875', 'G03.191.875', 'G03.295.693', 'G03.493.695'], ['D01.268.406.875', 'D01.362.340.875', 'D01.496.749.925']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Anatomy [A]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[Optimization of the intracavitary irradiation of patients with cancer of the endometrium].
|
Twelve variants of uterine cavity structure were defined on analysis of 52 hysterocervicograms performed in patients with cancer of the uterine body. Using the method of linear programming, the most effective schemes for the location of radiation sources were proposed to ensure optimum dose distribution in intracavitary irradiation. Sources I and II were the main contributors to dose distribution. The use of sequential radiation sources was appropriate in considerable sizes of the uterine cavity. In a spherically shaped uterine cavity dose distribution could be ensured by using only one source, located in the center.
|
['Brachytherapy', 'Female', 'Humans', 'Programming, Linear', 'Radiotherapy Dosage', 'Uterine Neoplasms', 'Uterus']
| 3,185,204
|
[['E02.815.150'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['L01.906.394.748'], ['E02.815.639'], ['C04.588.945.418.948', 'C13.351.500.852.762', 'C13.351.937.418.875'], ['A05.360.319.679']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Information Science [L]', 'Diseases [C]', 'Anatomy [A]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Characterization of the bovine salivary gland transcriptome associated with Mycobacterium avium subsp. paratuberculosis experimental challenge.
|
BACKGROUND: Mycobacterium avium subsp. paratuberculosis (MAP), the etiologic agent of Johne's disease is spread between cattle via the fecal-oral route, yet the functional changes in the salivary gland associated with infection remain uncharacterized. In this study, we hypothesized that experimental challenge with MAP would induce stable changes in gene expression patterns in the salivary gland that may shed light on the mucosal immune response as well as the regional variation in immune capacity of this extensive gland. Holstein-Friesian cattle were euthanized 33 months' post oral challenge with MAP strain CIT003 and both the parotid and mandibular salivary glands were collected from healthy control (n = 5) and MAP exposed cattle (n = 5) for histopathological and transcriptomic analysis.RESULTS: A total of 205, 21, 61, and 135 genes were significantly differentially expressed between control and MAP exposed cattle in dorsal mandibular (M1), ventral mandibular (M2), dorsal parotid (P1) and ventral parotid salivary glands (P2), respectively. Expression profiles varied between the structurally divergent parotid and mandibular gland sections which was also reflected in the enriched biological pathways identified. Changes in gene expression associated with MAP exposure were detected with significantly elevated expression of BoLA DR-ALPHA, BOLA-DRB3 and complement factors in MAP exposed cattle. In contrast, reduced expression of genes such as polymeric immunoglobin receptor (PIGR), TNFSF13, and the antimicrobial genes lactoferrin (LF) and lactoperoxidase (LPO) was detected in MAP exposed animals.CONCLUSIONS: This first analysis of the transcriptomic profile of salivary glands in cattle adds an important layer to our understanding of salivary gland immune function. Transcriptomic changes associated with MAP exposure have been identified including reduced LF and LPO. These critical antimicrobial and immunoregulatory proteins are known to be secreted into saliva and their downregulation may contribute to disease susceptibility. Future work will focus on the validation of their expression levels in saliva from additional cattle of known infection status as a potential strategy to augment disease diagnosis.
|
['Animals', 'Cattle', 'Gene Expression Profiling', 'Gene Ontology', 'Genomics', 'Mycobacterium avium subsp. paratuberculosis', 'Salivary Glands', 'Sequence Alignment', 'Sequence Analysis']
| 31,195,975
|
[['B01.050'], ['B01.050.150.900.649.313.500.380.271'], ['E05.393.332'], ['H01.158.273.343.249.099', 'H01.770.644.145.350.124', 'L01.224.050.375.480.500.500', 'L01.313.500.750.300.550.500.500', 'L01.453.245.945.079.500'], ['H01.158.273.180.350', 'H01.158.273.343.350'], ['B03.510.024.962.500.300.600', 'B03.510.460.400.410.552.552.300.600'], ['A03.556.500.760', 'A10.336.779', 'A14.549.760'], ['E05.393.751'], ['E05.393.760']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Information Science [L]', 'Anatomy [A]']
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
|
[Sampling studies in ambulatory quality assurance -- using the example of colonoscopy].
|
BACKGROUND: Sampling inspections are an approved instrument for assuring and promoting the quality of healthcare. Individual documentations of medical services are requested from physicians and randomly selected for quality rating by experienced peer reviewers. For example, sampling inspections are stipulated by law for certain ambulatory services (e.g., colonoscopies) delivered by SHI-authorised physicians in order to maintain professional performance standards of colonoscopists.OBJECTIVES: On behalf of the regional Association of Statutory Health Insurance Physicians (ASHIP) experienced colonoscopists regularly rate selected visual documentations (videotapes, photographs) of colonoscopies performed by SHI-authorised physicians in the ambulatory care sector. For anatomical reasons, however, a certain proportion of colonoscopies of inadequate quality will generally be tolerated. Whenever this value is exceeded, ASHIP may impose sanctions against the physician. The question is how sampling inspections have to be performed and dimensioned in order to ensure that the tests be sufficiently meaningful in terms of sensitivity and specificity.METHODS: Relevant sampling test parameters such as the false-positive rate (physicians are wrongly accused of inadequate quality) and the false-negative rate (existing deficiencies are not identified) are calculated. The calculations are performed analytically or, in the case of complex sampling test scenarios, numerically by means of computer simulations.RESULTS: The calculations show that single-stage sampling tests usually do not result in acceptable values for the false-positive and the false-negative rates. For example, a sampling test which requires the documentation of 20 colonoscopies will -- assuming some reasonable tolerance of inadequately performed colonoscopies -- result in a false-positive rate of 6% and a false-negative rate of 47%. The false-positive-rate, which is particularly relevant from a legal point of view, can be reduced by providing a two-stage sampling test. A significant reduction of the false-negative-rate may be achieved by (multiple) repetition of the single-stage sampling test and consideration of cumulative probabilities.CONCLUSIONS: In principle, sampling inspections permit statements in terms of probabilities only. In sampling inspections of healthcare quality false-negative rates are usually considered, i.e., the probability that the test is unable to identify existing quality deficiencies. However, false-positive rates also need to be considered in cases where sanctions may be imposed against the physician on the basis of a positive sampling test. Numerical calculations of false-positive and false-negative rates for simple and complex sampling test scenarios should be performed in order to choose the optimum procedure and dimension of a sampling test.
|
['Ambulatory Care', 'Colonoscopy', 'Delivery of Health Care', 'False Negative Reactions', 'False Positive Reactions', 'Humans', 'Quality Assurance, Health Care', 'Sampling Studies']
| 19,554,891
|
[['E02.760.106', 'N02.421.585.106'], ['E01.370.372.250.250.200', 'E01.370.388.250.250.250.160', 'E04.210.240.250.160', 'E04.502.250.250.250.160'], ['N04.590.374', 'N05.300'], ['E01.354.340'], ['E01.354.506'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['N04.761.700', 'N05.700'], ['E05.318.372.875', 'N05.715.360.330.875', 'N06.850.520.450.875']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]']
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Recurrent PPP2R1A Mutations in Uterine Cancer Act through a Dominant-Negative Mechanism to Promote Malignant Cell Growth.
|
Somatic missense mutations in the Ser/Thr protein phosphatase 2A (PP2A) Aá scaffold subunit gene PPP2R1A are among the few genomic alterations that occur frequently in serous endometrial carcinoma (EC) and carcinosarcoma, two clinically aggressive subtypes of uterine cancer with few therapeutic options. Previous studies reported that cancer-associated Aá mutants exhibit defects in binding to other PP2A subunits and contribute to cancer development by a mechanism of haploinsufficiency. Here we report on the functional significance of the most recurrent PPP2R1A mutations in human EC, which cluster in Aá HEAT repeats 5 and 7. Beyond predicted loss-of-function effects on the formation of a subset of PP2A holoenzymes, we discovered that Aá mutants behave in a dominant-negative manner due to gain-of-function interactions with the PP2A inhibitor TIPRL1. Dominant-negative Aá mutants retain binding to specific subunits of the B56/B' family and form substrate trapping complexes with impaired phosphatase activity via increased recruitment of TIPRL1. Accordingly, overexpression of the Aá mutants in EC cells harboring wild-type PPP2R1A increased anchorage-independent growth and tumor formation, and triggered hyperphosphorylation of oncogenic PP2A-B56/B' substrates in the GSK3â, Akt, and mTOR/p70S6K signaling pathways. TIPRL1 silencing restored GSK3â phosphorylation and rescued the EC cell growth advantage. Our results reveal how PPP2R1A mutations affect PP2A function and oncogenic signaling, illuminating the genetic basis for serous EC development and its potential control by rationally targeted therapies. Cancer Res; 76(19); 5719-31. ©2016 AACR.
|
['Animals', 'Cell Line, Tumor', 'Cell Proliferation', 'Cystadenocarcinoma, Serous', 'Endometrial Neoplasms', 'Female', 'Humans', 'Intracellular Signaling Peptides and Proteins', 'Mice', 'Mutation, Missense', 'Protein Phosphatase 2', 'Ribosomal Protein S6 Kinases, 70-kDa', 'TOR Serine-Threonine Kinases']
| 27,485,451
|
[['B01.050'], ['A11.251.210.190', 'A11.251.860.180'], ['G04.161.750', 'G07.345.249.410.750'], ['C04.557.470.200.025.480.240', 'C04.557.470.590.480.240'], ['C04.588.945.418.948.585', 'C13.351.500.852.762.200', 'C13.351.937.418.875.200'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.644.360', 'D12.776.476'], ['B01.050.150.900.649.313.992.635.505.500'], ['G05.365.590.650'], ['D08.811.277.352.650.625.706', 'D12.644.360.583', 'D12.776.476.561'], ['D08.811.913.696.620.682.700.862.249', 'D12.644.360.600.249', 'D12.776.476.600.249'], ['D08.811.913.696.620.682.700.931', 'D12.776.476.925']]
|
['Organisms [B]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Chemicals and Drugs [D]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Probing the substrate specificity for lipases. II. Kinetic and modeling studies on the molecular recognition of 2-arylpropionic esters by Candida rugosa and Rhizomucor miehei lipases.
|
Racemic arylpropionic esters 1-3, precursors of therapeutically important non-steroidal antiinflammatory drugs, were subjected to hydrolyses in the presence of either Candida rugosa or Rhizomucor miehei crude lipases. The hydrolyses of 1 and 2 proved to be highly enantioselective, whereas 3 was not transformed at all. Both the substrate specificity and the enantioselectivity of these lipases were explained through a molecular modeling study involving docking experiments between 1-3 and the amino acids forming the enzymes active-sites, whose three dimensional structures were obtained from X-ray crystallographic data, followed by extensive conformational analysis on their computer-generated complexes. The results of this study also account for the high enantioselective and good yielding hydrolysis of 3 (as the corresponding 2-chloroethyl ester) catalyzed by CRL pretreated with 2-propanol, recently reported in the literature, and lead to admit that such a treatment may operate very deep conformational changes on the amino acids of the enzyme active-site.
|
['Binding Sites', 'Candida', 'Crystallography, X-Ray', 'Kinetics', 'Lipase', 'Models, Molecular', 'Molecular Structure', 'Mucorales', 'Propionates', 'Protein Conformation', 'Substrate Specificity']
| 9,048,908
|
[['G02.111.570.120'], ['B01.300.107.795.095', 'B01.300.381.147', 'B01.300.930.176'], ['E05.196.309.742.225'], ['G01.374.661', 'G02.111.490'], ['D08.811.277.352.100.400'], ['E05.599.595'], ['G02.111.570', 'G02.466'], ['B01.300.300.500'], ['D02.241.081.751', 'D10.251.400.706'], ['G02.111.570.820.709'], ['G02.111.835']]
|
['Phenomena and Processes [G]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[Tuberculosis: from compulsory admission to compulsory treatment].
|
Tuberculosis is still considered to be a threat to public health in the Netherlands. The Dutch Public Health Act enables the mandatory isolation of contagious patients who are not willing to be treated. However, this act does not mean that patients can be treated against their will. Another act, the Dutch Medical Treatment Act, regulates the contract between doctor and patient. According to this act, only patients who are mentally incompetent can be treated against their will. We describe two patients with contagious tuberculosis who are mentally incompetent. This article explains the steps which, in accordance with both acts, must be followed before starting appropriate treatment.
|
['Aged', 'Hospitalization', 'Humans', 'Mandatory Programs', 'Mental Competency', 'Middle Aged', 'Netherlands', 'Tuberculosis']
| 30,182,637
|
[['M01.060.116.100'], ['E02.760.400', 'N02.421.585.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['I01.880.604.622', 'N03.706.657', 'N04.452.521'], ['F01.590', 'F02.410', 'I01.880.604.583.530', 'N03.706.535.625'], ['M01.060.116.630'], ['Z01.542.651'], ['C01.150.252.410.040.552.846']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Psychiatry and Psychology [F]', 'Geographicals [Z]', 'Diseases [C]']
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 1
|
[Pathogenesis of recurrences of acute gastroduodenal ulcerous bleedings].
|
Based on analysis of morphologic data, oxygen regime and redox potential it is demonstrated that progressive ischemic necrosis in periulcerous zone is the basis of recurrence of gastroduodenal ulcerous bleedings. Systemic hemostatic therapy, antisecretion drugs, endoscopic methods of hemostasis don't guarantee absence of bleeding recurrence. Prognosis of recurrence of gastroduodenal ulcerous bleedings must be based on evaluation of clinical and endoscopic data. Partial pressure of oxygen and redox potential in ulcer's crater are the objective criteria of threat of bleeding's recurrence.
|
['Acute Disease', 'Duodenum', 'Homeostasis', 'Humans', 'Ischemia', 'Multivariate Analysis', 'Necrosis', 'Oxidative Stress', 'Peptic Ulcer Hemorrhage', 'Prognosis', 'Recurrence', 'Stomach']
| 15,159,759
|
[['C23.550.291.125'], ['A03.556.124.684.124', 'A03.556.875.249'], ['G07.410'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C23.550.513'], ['E05.318.740.150.500', 'N05.715.360.750.125.500', 'N06.850.520.830.150.500'], ['C23.550.717'], ['G03.673', 'G07.775.750'], ['C06.405.227.700', 'C23.550.414.788.700'], ['E01.789'], ['C23.550.291.937'], ['A03.556.875.875']]
|
['Diseases [C]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Inter-individual differences in the experience of negative emotion predict variations in functional brain architecture.
|
Current evidence suggests that two spatially distinct neuroanatomical networks, the dorsal attention network (DAN) and the default mode network (DMN), support externally and internally oriented cognition, respectively, and are functionally regulated by a third, frontoparietal control network (FPC). Interactions among these networks contribute to normal variations in cognitive functioning and to the aberrant affective profiles present in certain clinical conditions, such as major depression. Nevertheless, their links to non-clinical variations in affective functioning are still poorly understood. To address this issue, we used fMRI to measure the intrinsic functional interactions among these networks in a sample of predominantly younger women (N=162) from the Human Connectome Project. Consistent with the previously documented dichotomous motivational orientations (i.e., withdrawal versus approach) associated with sadness versus anger, we hypothesized that greater sadness would predict greater DMN (rather than DAN) functional dominance, whereas greater anger would predict the opposite. Overall, there was evidence of greater DAN (rather than DMN) functional dominance, but this pattern was modulated by current experience of specific negative emotions, as well as subclinical depressive and anxiety symptoms. Thus, greater levels of currently experienced sadness and subclinical depression independently predicted weaker DAN functional dominance (i.e., weaker DAN-FPC functional connectivity), likely reflecting reduced goal-directed attention towards the external perceptual environment. Complementarily, greater levels of currently experienced anger and subclinical anxiety predicted greater DAN functional dominance (i.e., greater DAN-FPC functional connectivity and, for anxiety only, also weaker DMN-FPC coupling). Our findings suggest that distinct affective states and subclinical mood symptoms have dissociable neural signatures, reflective of the symbiotic relationship between cognitive processes and emotional states.
|
['Adult', 'Affect', 'Anger', 'Anxiety', 'Brain', 'Brain Mapping', 'Female', 'Humans', 'Individuality', 'Magnetic Resonance Imaging', 'Male', 'Neural Pathways', 'Young Adult']
| 26,302,674
|
[['M01.060.116'], ['F01.470.047'], ['F01.470.093'], ['F01.470.132'], ['A08.186.211'], ['E01.370.350.578.875.500', 'E01.370.376.537.625.500', 'E05.629.875.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F01.752.488'], ['E01.370.350.825.500'], ['A08.612'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Psychiatry and Psychology [F]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]']
| 1
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Identification of a gonadotropin-releasing hormone-responsive region in the glycoprotein hormone alpha-subunit promoter.
|
Transcriptional regulation of the glycoprotein hormone alpha-subunit gene has been studied extensively in placental cells, but much less is known about its regulation in the pituitary gland. In this study, transcriptional control of the human alpha-subunit gene by GnRH was analyzed using transient expression assays in primary cultures of rat pituitary cells using alpha promoter constructs linked to a luciferase reporter gene. Deletion mutants between -846 and -156 basepairs (bp) had little effect on basal expression, but further deletion to -132 bp reduced basal activity by approximately 50%. Deletion of a cAMP response element between -132 and -99 bp caused a marked loss of basal activity, reducing expression to that of background luciferase activity. The same constructs were analyzed for cAMP responsiveness in primary pituitary cells. The degree of stimulation with 1 mM 8-bromo-cAMP (3.6- to 6.0-fold) was relatively unaffected by deletions from -846 to -132 bp, whereas cAMP stimulation was decreased by further deletion to -99 bp, consistent with the presence of previously defined cAMP response elements in this region of the alpha promoter. GnRH stimulation of alpha promoter activity was highly dependent upon the time of hormone addition after transfection, being most effective when added soon after transfection. Under optimal conditions, GnRH stimulated -846 alpha LUC expression by 20-fold. GnRH responsiveness was retained with deletion to -346 bp, but it was decreased by 55% after deletion to -280 bp and by 79% with deletion to -244 bp.(ABSTRACT TRUNCATED AT 250 WORDS)
|
['8-Bromo Cyclic Adenosine Monophosphate', 'Animals', 'Gene Expression Regulation', 'Glycoprotein Hormones, alpha Subunit', 'Gonadotropin-Releasing Hormone', 'Humans', 'Pituitary Gland, Anterior', 'Promoter Regions, Genetic', 'Rats', 'Recombinant Fusion Proteins', 'Regulatory Sequences, Nucleic Acid', 'Sequence Deletion', 'Transcription, Genetic', 'Transfection']
| 1,282,668
|
[['D03.633.100.759.646.138.395.225', 'D13.695.462.200.225', 'D13.695.667.138.395.225', 'D13.695.827.068.395.225'], ['B01.050'], ['G05.308'], ['D06.472.699.322.326.562', 'D06.472.699.322.576.288.750', 'D06.472.699.322.576.463.249', 'D06.472.699.631.525.343.288.750', 'D06.472.699.631.525.343.463.249', 'D06.472.699.631.525.883.249', 'D06.472.699.649.367.562', 'D12.644.548.691.525.343.288.750', 'D12.644.548.691.525.343.463.249', 'D12.644.548.691.525.883.249', 'D12.644.548.726.367.562'], ['D06.472.699.327.740.320', 'D12.644.400.400.740.320', 'D12.644.456.460', 'D12.644.548.365.740.320', 'D12.776.631.650.405.740.320'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A06.300.747.500', 'A06.688.357.750.500', 'A08.186.211.180.497.352.435.500.500', 'A08.186.211.200.317.357.352.435.500.500', 'A08.713.357.750.500'], ['G02.111.570.080.689.675', 'G05.360.080.689.675', 'G05.360.340.024.340.137.750.680'], ['B01.050.150.900.649.313.992.635.505.700'], ['D12.776.828.300'], ['G02.111.570.080.689', 'G05.360.080.689'], ['G05.365.590.762', 'G05.558.800'], ['G02.111.873', 'G05.297.700'], ['E05.393.350.810', 'G05.728.860']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[A study on postoperative long-term continuous immuno-chemotherapy with PSK and 5-FUDS for advanced gastric and colo-rectal cancers].
|
Since 1976, postoperative long term immunochemotherapy (PLIC) with PSK and 5-FU Dry Syrup (5-FUDS) was performed after tumor resection in gastric and colorectal cancer patients. Successful continuation over one year of the therapy was done in 40 cases at stages III and IV (+V) in the period between January 1976 and April 1979. These cases were analyzed and following results were obtained. Two-and 3-year survival rates were 80% and 65% respectively when the total cases of one year survival was taken as 100%. However, there were recurrent or tumor-bearing patients being treated by PLIC and, if they had died within the respective years, 2-and 3-year survival rates would have been reduced to 67.5% and 32.5%, respectively. These survival rates were roughly similar as those of historical control group, in which no immuno-chemotherapy was done. Characteristic features of non-specific immunological parameters seen in the recurrent or tumor-bearing cases of relatively good clinical course were improvement in T cell percentage, reduction or stop of in IgG-FcR (+) T cell percentage and maintenance of a good nutritional condition.
|
['Aged', 'Antibiotics, Antineoplastic', 'Colonic Neoplasms', 'Drug Therapy, Combination', 'Fluorouracil', 'Humans', 'Middle Aged', 'Mitomycin', 'Mitomycins', 'Postoperative Period', 'Proteoglycans', 'Rectal Neoplasms', 'Stomach Neoplasms']
| 6,411,000
|
[['M01.060.116.100'], ['D27.505.954.248.106'], ['C04.588.274.476.411.307.180', 'C06.301.371.411.307.180', 'C06.405.249.411.307.180', 'C06.405.469.158.356.180', 'C06.405.469.491.307.180'], ['E02.319.310'], ['D03.383.742.698.875.404'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['D02.806.400.249.350', 'D03.383.097.500.350', 'D03.633.100.473.412.249.350'], ['D02.806.400.249', 'D03.383.097.500', 'D03.633.100.473.412.249'], ['E04.614.750', 'N02.421.585.753.750'], ['D09.698.735', 'D12.776.395.650'], ['C04.588.274.476.411.307.790', 'C06.301.371.411.307.790', 'C06.405.249.411.307.790', 'C06.405.469.491.307.790', 'C06.405.469.860.180.500'], ['C04.588.274.476.767', 'C06.301.371.767', 'C06.405.249.767', 'C06.405.748.789']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Health Care [N]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Investigating the role of mitochondrial haplogroups in genetic predisposition to meningococcal disease.
|
BACKGROUND AND AIMS: Meningococcal disease remains one of the most important infectious causes of death in industrialized countries. The highly diverse clinical presentation and prognosis of Neisseria meningitidis infections are the result of complex host genetics and environmental interactions. We investigated whether mitochondrial genetic background contributes to meningococcal disease (MD) susceptibility.METHODOLOGY/PRINCIPAL FINDINGS: Prospective controlled study was performed through a national research network on MD that includes 41 Spanish hospitals. Cases were 307 paediatric patients with confirmed MD, representing the largest series of MD patients analysed to date. Two independent sets of ethnicity-matched control samples (CG1 [N = 917]), and CG2 [N = 616]) were used for comparison. Cases and controls underwent mtDNA haplotyping of a selected set of 25 mtDNA SNPs (mtSNPs), some of them defining major European branches of the mtDNA phylogeny. In addition, 34 ancestry informative markers (AIMs) were genotyped in cases and CG2 in order to monitor potential hidden population stratification. Samples of known African, Native American and European ancestry (N = 711) were used as classification sets for the determination of ancestral membership of our MD patients. A total of 39 individuals were eliminated from the main statistical analyses (including fourteen gypsies) on the basis of either non-Spanish self-reported ancestry or the results of AIMs indicating a European membership lower than 95%. Association analysis of the remaining 268 cases against CG1 suggested an overrepresentation of the synonym mtSNP G11719A variant (Pearson's chi-square test; adjusted P-value = 0.0188; OR [95% CI] = 1.63 [1.22-2.18]). When cases were compared with CG2, the positive association could not be replicated. No positive association has been observed between haplogroup (hg) status of cases and CG1/CG2 and hg status of cases and several clinical variants.CONCLUSIONS: We did not find evidence of association between mtSNPs and mtDNA hgs with MD after carefully monitoring the confounding effect of population sub-structure. MtDNA variability is particularly stratified in human populations owing to its low effective population size in comparison with autosomal markers and therefore, special care should be taken in the interpretation of seeming signals of positive associations in mtDNA case-control association studies.
|
['Chi-Square Distribution', 'Child, Preschool', 'DNA, Mitochondrial', 'Demography', 'Female', 'Genetic Predisposition to Disease', 'Genetics, Population', 'Haplotypes', 'Humans', 'Male', 'Meningococcal Infections', 'Mitochondria', 'Phylogeny']
| 20,019,817
|
[['E05.318.740.994.300', 'G17.820.300', 'N05.715.360.750.750.200', 'N06.850.520.830.994.300'], ['M01.060.406.448'], ['D13.444.308.283.225'], ['I01.240', 'N01.224', 'N06.850.505.400'], ['C23.550.291.687.500', 'G05.380.355'], ['H01.158.273.343.335'], ['G05.380.360'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C01.150.252.400.625.549'], ['A11.284.430.214.190.875.564', 'A11.284.835.626'], ['G05.697', 'G16.075.605', 'L01.100.697']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Named Groups [M]', 'Chemicals and Drugs [D]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Diseases [C]', 'Disciplines and Occupations [H]', 'Organisms [B]', 'Anatomy [A]', 'Information Science [L]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 1
| 0
| 1
| 1
| 1
| 0
|
The International Decision Support Initiative Reference Case for Economic Evaluation: An Aid to Thought.
|
BACKGROUND: Policymakers in high-, low-, and middle-income countries alike face challenging choices about resource allocation in health. Economic evaluation can be useful in providing decision makers with the best evidence of the anticipated benefits of new investments, as well as their expected opportunity costs-the benefits forgone of the options not chosen. To guide the decisions of health systems effectively, it is important that the methods of economic evaluation are founded on clear principles, are applied systematically, and are appropriate to the decision problems they seek to inform.METHODS: The Bill and Melinda Gates Foundation, a major funder of economic evaluations of health technologies in low- and middle-income countries (LMICs), commissioned a "reference case" through the International Decision Support Initiative (iDSI) to guide future evaluations, and improve both the consistency and usefulness to decision makers.RESULTS: The iDSI Reference Case draws on previous insights from the World Health Organization, the US Panel on Cost-Effectiveness in Health Care, and the UK National Institute for Health and Care Excellence. Comprising 11 key principles, each accompanied by methodological specifications and reporting standards, the iDSI Reference Case also serves as a means of identifying priorities for methods research, and can be used as a framework for capacity building and technical assistance in LMICs.CONCLUSIONS: The iDSI Reference Case is an aid to thought, not a substitute for it, and should not be followed slavishly without regard to context, culture, or history. This article presents the iDSI Reference Case and discusses the rationale, approach, components, and application in LMICs.
|
['Capacity Building', 'Cost of Illness', 'Cost-Benefit Analysis', 'Decision Making', 'Developing Countries', 'Global Health', 'Health Policy', 'Humans', 'Uncertainty']
| 27,987,641
|
[['N02.138', 'N04.452.105'], ['N03.219.151.165', 'N05.715.360.300.800.438.375.182', 'N06.850.520.308.980.438.475.046'], ['N03.219.151.125'], ['F02.463.785.373'], ['I01.615.500.300'], ['H02.403.371', 'N01.400.337'], ['I01.655.500.608.400', 'I01.880.604.825.608.400', 'N03.623.500.608.428'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.740.600.900', 'F02.463.785.373.820', 'G17.680.875', 'N05.715.360.750.625.850', 'N06.850.520.830.600.900']]
|
['Health Care [N]', 'Psychiatry and Psychology [F]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Disciplines and Occupations [H]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 0
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 1
| 0
|
Label-free colorimetric detection of Hg²⁺ based on Hg²⁺-triggered exonuclease III-assisted target recycling and DNAzyme amplification.
|
This work reported a label-free colorimetric assay for sensitive detection of Hg(2+) based on Hg(2+)-triggered hairpin DNA probe (H-DNA) termini-binding and exonuclease ? (Exo ?)-assisted target recycling, as well as hemin/G-quadruplex (DNAzyme) signal amplification. The specific binding of free Hg(2+) with the thymine-thymine (T-T) mismatches termini of H-DNA could immediately trigger the Exo ? digestion, and then set free G-quadruplex segments and Hg(2+). The Exo ? impellent recycling of ultratrace Hg(2+) produced numerous G-quadruplexes. The corresponding DNAzymes catalyzed efficiently the H2O2-mediated oxidation of the ABTS(2-) to the colored product in the presence of hemin. Using the color change as the output signal, and the Exo ?-aided Hg(2+) recycling and DNAzyme as the signal amplifier, the ultrasensitive assay system successfully achieved visual detection of Hg(2+) as low as 1.0 nM by the naked eye, and was suitable for field monitoring. The calibration curve was linear in the range of 50.0 pM to 20.0 nM for Hg(2+) (R=0.9962) with a detection limit of 10.0 pM. Moreover, this proposed strategy showed excellent selectivity, portability and low-cost, and was successfully applied to colorimetric detection of Hg(2+) in laboratory tap water and Jialing river water samples.
|
['Biosensing Techniques', 'Colorimetry', 'DNA Probes', 'Exodeoxyribonucleases', 'G-Quadruplexes', 'Hydrogen Peroxide', 'Limit of Detection', 'Mercury', 'Nucleic Acid Amplification Techniques']
| 25,590,972
|
[['E05.601.043'], ['E05.196.922.250'], ['D13.444.600.223', 'D27.505.259.750.600.223', 'D27.720.470.530.600.223'], ['D08.811.277.352.335.375', 'D08.811.277.352.365.290'], ['G02.111.570.820.486.550', 'G05.360.580.550'], ['D01.248.497.158.685.750.424', 'D01.339.431.374.424', 'D01.650.550.750.400', 'D02.389.338.253'], ['E05.318.740.872.374', 'N05.715.360.750.725.500', 'N06.850.520.830.872.500'], ['D01.268.556.504', 'D01.268.956.437', 'D01.552.544.504'], ['E05.393.620']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Health Care [N]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Status of the genus Cyrnea (Nematoda: Spiruroidea) in wild turkeys from the southeastern United States.
|
Two species of Cyrnea are reported from 706 wild turkeys (Meleagris gallopavo) from 25 localities in 9 southeastern states. Cyrnea (Cyrnea) neeli sp. n. in birds from Alabama and Florida is differentiated primarily by distal processes of the left spicule and spicule lengths. Specimens from wild turkeys previously reported as C. eurycerca are identified as C. coloni. The configuration of caudal papillae of C. colini is redescribed. Neither species occurred in birds from montainous regions, and C. neeli sp. n. was restricted to Florida and southern Alabama. Poults less than 1-month old were not infected. Infections peaked in early fall and then declined rapidly.
|
['Animals', 'Female', 'Male', 'Spiruroidea', 'Turkeys', 'United States']
| 859,088
|
[['B01.050'], ['B01.050.500.500.294.400.937.700.680'], ['B01.050.150.900.248.350.800', 'B01.050.150.900.248.690.800'], ['Z01.107.567.875']]
|
['Organisms [B]', 'Geographicals [Z]']
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
|
Inversion of chromosome 3 in a case of chronic myelogenous leukemia with abnormal thrombopoiesis.
|
A patient with chronic myelogenous leukemia (CML) associated with a remarkable increase of micromegakaryocytes in bone marrow was revealed to have an abnormality of a long arm of chromosome #3, i.e., inv(3)(q21q26), in addition to a complex Ph translocation: t(9;22;15)(q34;q11;q22). Although several cases of acute leukemia with inv(3)(q21q26) and abnormal megakaryocytes have been reported, this is the first case in which the association of inv(3)(q21q26) with a megakaryocytic abnormality was observed in a patient with CML. Our findings suggest that this structural rearrangement may be more specifically associated with abnormal thrombopoiesis than are other structural anomalies of 3q.
|
['Blood Platelets', 'Chromosome Inversion', 'Chromosomes, Human, 1-3', 'Hematopoiesis', 'Humans', 'Leukemia, Myeloid', 'Male', 'Megakaryocytes', 'Middle Aged', 'Philadelphia Chromosome', 'Platelet Count']
| 3,455,854
|
[['A11.118.188', 'A15.145.229.188'], ['C23.550.210.190', 'G05.365.590.175.190', 'G05.365.590.770.500', 'G05.558.805.500'], ['A11.284.187.520.300.235', 'G05.360.162.520.300.235'], ['G04.152.825', 'G09.188.343'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C04.557.337.539'], ['A11.148.479', 'A15.378.316.479'], ['M01.060.116.630'], ['A11.284.187.520.300.325.345.500', 'A11.284.187.520.300.505.515.500', 'C23.550.210.870.680', 'G05.360.162.520.300.325.345.700', 'G05.360.162.520.300.505.515.700', 'G05.365.590.175.870.680'], ['E01.370.225.500.195.107.740', 'E01.370.225.625.107.700', 'E01.370.225.625.625.625', 'E05.200.500.195.107.740', 'E05.200.625.107.700', 'E05.200.625.625.625', 'E05.242.195.107.740', 'G04.140.107.740', 'G09.188.105.700']]
|
['Anatomy [A]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
The SV40 late protein VP4 is a viroporin that forms pores to disrupt membranes for viral release.
|
Nonenveloped viruses are generally released by the timely lysis of the host cell by a poorly understood process. For the nonenveloped virus SV40, virions assemble in the nucleus and then must be released from the host cell without being encapsulated by cellular membranes. This process appears to involve the well-controlled insertion of viral proteins into host cellular membranes rendering them permeable to large molecules. VP4 is a newly identified SV40 gene product that is expressed at late times during the viral life cycle that corresponds to the time of cell lysis. To investigate the role of this late expressed protein in viral release, water-soluble VP4 was expressed and purified as a GST fusion protein from bacteria. Purified VP4 was found to efficiently bind biological membranes and support their disruption. VP4 perforated membranes by directly interacting with the membrane bilayer as demonstrated by flotation assays and the release of fluorescent markers encapsulated into large unilamellar vesicles or liposomes. The central hydrophobic domain of VP4 was essential for membrane binding and disruption. VP4 displayed a preference for membranes comprised of lipids that replicated the composition of the plasma membranes over that of nuclear membranes. Phosphatidylethanolamine, a lipid found at high levels in bacterial membranes, was inhibitory against the membrane perforation activity of VP4. The disruption of membranes by VP4 involved the formation of pores of ?3 nm inner diameter in mammalian cells including permissive SV40 host cells. Altogether, these results support a central role of VP4 acting as a viroporin in the perforation of cellular membranes to trigger SV40 viral release.
|
['Cell Membrane', 'Fluorescent Antibody Technique', 'Glutathione Transferase', 'Liposomes', 'Phosphatidylethanolamines', 'Porins', 'Porosity', 'Recombinant Fusion Proteins', 'Sequence Deletion', 'Simian virus 40', 'Viral Structural Proteins', 'Virus Release']
| 21,738,474
|
[['A11.284.149'], ['E01.370.225.500.607.512.240', 'E01.370.225.750.551.512.240', 'E05.200.500.607.512.240', 'E05.200.750.551.512.240', 'E05.478.583.375'], ['D08.811.913.225.500'], ['D25.479.517', 'D26.255.260.517', 'J01.637.051.479.517', 'J01.637.087.500.517'], ['D10.570.755.375.760.400.840'], ['D12.776.157.530.400.500', 'D12.776.543.550.450.730', 'D12.776.543.585.400.730'], ['G01.374.710'], ['D12.776.828.300'], ['G05.365.590.762', 'G05.558.800'], ['B04.280.210.700.615.700', 'B04.613.204.670.615.700'], ['D12.776.964.970'], ['G06.920.913']]
|
['Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Technology, Industry, and Agriculture [J]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Cloning, expression, purification and crystallization as well as X-ray fluorescence and preliminary X-ray diffraction analyses of human ADP-ribosylhydrolase 1.
|
Human ADP-ribosylhydrolase 1 (hARH1, ADPRH) cleaves the glycosidic bond of ADP-ribose attached to an Arg residue of a protein. hARH1 has been cloned, expressed heterologously in Escherichia coli, purified and crystallized in complex with K(+) and ADP. The orthorhombic crystals contained one monomer per asymmetric unit, exhibited a solvent content of 43% and diffracted X-rays to a resolution of 1.9 A. A prerequisite for obtaining well diffracting crystals was the performance of X-ray fluorescence analysis on poorly diffracting apo hARH1 crystals, which revealed the presence of trace amounts of K(+) in the crystal. Adding K-ADP to the crystallization cocktail then resulted in a crystal of different morphology and with dramatically improved diffraction properties.
|
['Cloning, Molecular', 'Crystallization', 'Crystallography, X-Ray', 'Enzymes', 'Fluorescence', 'Gene Expression', 'N-Glycosyl Hydrolases', 'X-Rays']
| 19,407,395
|
[['E05.393.220'], ['E05.196.300', 'G02.171'], ['E05.196.309.742.225'], ['D08.811'], ['G01.358.500.505.650.665.500', 'G01.590.540.665.500'], ['G05.297'], ['D08.811.277.450.430'], ['G01.358.500.505.970', 'G01.750.250.970', 'G01.750.750.918']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Effects on varying intravenous lipid emulsions on the small bowel epithelium in a mouse model of parenteral nutrition.
|
BACKGROUND: Injectable fat emulsions (FEs) are a clinically dependable source of essential fatty acids (FA). ù-6 FA is associated with an inflammatory response. Medium-chain triglycerides (MCT, ù-3 FA), fish oil, and olive oil are reported to decrease the inflammatory response. However, the effect of these lipids on the gastrointestinal tract has not been well studied. To address this, we used a mouse model of parenteral nutrition (PN) and hypothesized that a decrease in intestinal inflammation would be seen when either fish oil and MCT or olive oil were added.METHODS: Three FEs were studied in adult C57BL/6 mice via intravenous cannulation: standard soybean-based FE (SBFE), 80% olive oil -supplemented FE (OOFE), or a combination of a soybean oil, MCT, olive oil, and fish oil emulsion (SMOF). PN was given for 7 days, small bowel mucosa-derived cytokines, animal survival rate, epithelial cell (EC) proliferation and apoptosis rates, intestinal barrier function and mucosal FA composition were analyzed.RESULTS: Compared to the SBFE and SMOF groups, the best survival, highest EC proliferation and lowest EC apoptosis rates were observed in the OOFE group; and associated with the lowest levels of tumor necrosis factor-á, interleukin-6, and interleukin-1â expression. Jejunal FA content showed higher levels of eicosapentaenoic and docosapentaenoic acid in the SMOF group and the highest arachidonic acid in the OOFE group.CONCLUSION: The study showed that PN containing OOFE had beneficial effects to small bowel health and animal survival. Further investigation may help to enhance bowel integrity in patients restricted to PN.
|
['Animals', 'Cytokines', 'Dietary Fats', 'Dietary Supplements', 'Epithelial Cells', 'Fat Emulsions, Intravenous', 'Fatty Acids', 'Fatty Acids, Omega-3', 'Fatty Acids, Omega-6', 'Fish Oils', 'Inflammation', 'Intestinal Diseases', 'Intestinal Mucosa', 'Intestine, Small', 'Male', 'Mice', 'Mice, Inbred C57BL', 'Models, Animal', 'Olive Oil', 'Parenteral Nutrition', 'Plant Oils', 'Soybean Oil', 'Triglycerides']
| 23,757,306
|
[['B01.050'], ['D12.644.276.374', 'D12.776.467.374', 'D23.529.374'], ['D10.212.302', 'G07.203.300.375', 'J02.500.375'], ['G07.203.300.456', 'J02.500.456'], ['A11.436'], ['D26.255.165.260.270', 'D26.776.708.733.500', 'D27.505.954.578.733.500', 'D27.720.752.733.500'], ['D10.251'], ['D10.212.302.380.410', 'D10.251.355.337', 'D10.627.430.450'], ['D10.251.355.343'], ['D10.627.430'], ['C23.550.470'], ['C06.405.469'], ['A03.556.124.369', 'A10.615.550.444'], ['A03.556.124.684'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.420', 'B01.050.150.900.649.313.992.635.505.500.400.420'], ['E05.598'], ['D10.212.302.380.580', 'D10.212.507.650', 'D10.627.700.728', 'G07.203.300.375.400.500', 'J02.500.375.400.500'], ['E02.421.505', 'E02.642.500.505'], ['D10.627.700', 'D20.215.784.750'], ['D10.212.302.380.800', 'D10.212.507.800', 'D10.627.700.880', 'D20.215.784.750.880', 'G07.203.300.375.400.750', 'J02.500.375.400.750'], ['D10.351.801']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Technology, Industry, and Agriculture [J]', 'Anatomy [A]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Meditation matters.
|
Meditation is a healing discipline that can lead to the deepest awareness of the present moment through quieting the mind. And it can have positive physical and emotional effects.
|
['Attitude of Health Personnel', 'Health Promotion', 'Humans', 'Meditation', 'Nurses', 'Self Care', 'Self Concept', 'Self Psychology', 'Spirituality']
| 17,569,370
|
[['F01.100.050', 'N05.300.100'], ['I02.233.332.445', 'N02.421.726.407.579'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.190.525.374', 'E02.190.901.455', 'F04.754.137.750.500'], ['M01.526.485.650', 'N02.360.650'], ['E02.900', 'I03.050.563', 'N02.421.784.680'], ['F01.752.747.792'], ['F02.739.794.837'], ['F02.880.705', 'K01.844.664.500']]
|
['Psychiatry and Psychology [F]', 'Health Care [N]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Named Groups [M]', 'Humanities [K]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 0
|
Extent of the tetrodotoxin induced blockade examined by pupillary paralysis elicited by intracerebral injection of the drug.
|
Spatial extent and duration of the functional blockade elicited by intracerebral injection of tetrodotoxin (TTX) was examined in rats anesthetized with pentobarbital. Pupillary diameter was measured under dissecting microscope before and up to 24 h after injection of TTX (10 ng/l microliters saline) into or 1.0, 1.5 and 2.0 mm lateral from the Edinger-Westphal nucleus. TTX administration elicited mydriasis the latency of which was directly and amplitude indirectly proportional to the target-injection distance. The maximum mydriasis attained 3.4 mm, lasted 2 h and slowly decayed over subsequent 20 h. Impulse transmission and conduction was blocked in a spherical volume of tissue about 3 mm in diameter the development of which could be approximated by diffusion from an instantaneous point source. Completeness and full reversibility make the TTX block a convenient research tool.
|
['Animals', 'Brain', 'Male', 'Microinjections', 'Mydriasis', 'Oculomotor Nerve', 'Pupil', 'Rats', 'Stereotaxic Techniques', 'Tetrodotoxin']
| 2,026,211
|
[['B01.050'], ['A08.186.211'], ['E02.319.267.530.690', 'E05.591.570'], ['C11.710.570'], ['A08.800.050.050.650', 'A08.800.050.600.475', 'A08.800.800.060.650', 'A08.800.800.120.600'], ['A09.371.060.450.780', 'A09.371.894.513.780'], ['B01.050.150.900.649.313.992.635.505.700'], ['E04.525.800', 'E05.873'], ['D03.633.100.786.910', 'D23.946.580.910']]
|
['Organisms [B]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Chemicals and Drugs [D]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Training surgically competent doctors for South African rural settings.
|
The training of surgically competent doctors for South African rural settings is governed by several factors and determinants which include population demographics, distribution of doctors, infrastructural development and socio-economic conditions of the communities. Demands will be influenced by the disease profile, available facilities and academic support. Training options will include community-oriented/based (undergraduate) curriculum and restructured internship training period and acquisition of skills. Postgraduate diplomas and fellowships and continuing medical education and academic support will form an integral part of the training.
|
['Education, Medical, Continuing', 'Education, Medical, Graduate', 'Education, Medical, Undergraduate', 'General Surgery', 'Humans', 'Internship and Residency', 'Physician Incentive Plans', 'Physicians, Family', 'Rural Population', 'South Africa']
| 9,429,336
|
[['I02.358.212.350', 'I02.358.399.250'], ['I02.358.337.350', 'I02.358.399.350'], ['I02.358.399.450'], ['H02.403.810.300'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['I02.358.337.350.500', 'I02.358.399.350.750'], ['N04.452.677.740'], ['M01.526.485.810.770', 'N02.360.810.770'], ['N01.600.725'], ['Z01.058.290.175.735']]
|
['Anthropology, Education, Sociology, and Social Phenomena [I]', 'Disciplines and Occupations [H]', 'Organisms [B]', 'Health Care [N]', 'Named Groups [M]', 'Geographicals [Z]']
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
| 0
| 1
| 1
| 1
|
Research to Support the Development of a Campaign to Increase Physical Activity Among Low-Income, Urban, Diverse, Inactive Teens.
|
OBJECTIVE: To ascertain inactive teens' insights regarding the types of physical activities (PAs) they would be willing to do, and to inform a Supplemental Nutrition Assistance Program-Education PA social marketing campaign targeting this audience.DESIGN: Formative, qualitative research via focus groups.SETTING: Low-income, urban New Jersey areas between September, 2013 and April, 2014.PARTICIPANTS: Low-income, urban, ethnically diverse, inactive teens.PHENOMENON OF INTEREST: Teens' favored PAs and insights into how to develop a successful marketing campaign.ANALYSIS: Edited audio-transcriptions were coded and a constant comparative analysis was employed to identify emergent themes.RESULTS: Data from 5 focus groups' teens (n = 31), 58% of whom were Hispanic, 23% of whom were African American, and 19% of whom were of mixed race, revealed 3 themes. To be appealing, PAs (1) must be fun (eg, dancing, with friends and families) and (2) need to be comfortable (indoors, not sweaty, not physically competitive or embarrassing), and (3) they must be promoted by "cool" and relatable people (eg, teens like themselves or young comedians).CONCLUSIONS AND IMPLICATIONS: Nutrition and health educators and social marketers may be well advised to consider the unique preferences of inactive teens to improve their PA levels. Additional research in varied geographic regions is advisable.
|
['Adolescent', 'African Americans', 'Exercise', 'Focus Groups', 'Health Promotion', 'Hispanic Americans', 'Humans', 'Poverty', 'Psychology, Adolescent', 'Qualitative Research', 'Sedentary Behavior']
| 30,910,316
|
[['M01.060.057'], ['M01.686.508.100.100', 'M01.686.754.100'], ['G11.427.410.698.277', 'I03.350'], ['E05.318.308.112', 'N05.715.360.300.269', 'N06.850.520.308.112'], ['I02.233.332.445', 'N02.421.726.407.579'], ['M01.686.754.441'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['I01.880.735.634', 'I01.880.853.996.535', 'N01.824.600'], ['F04.096.628.065'], ['H01.770.644.241.850'], ['F01.145.749', 'F01.829.458.705']]
|
['Named Groups [M]', 'Phenomena and Processes [G]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Psychiatry and Psychology [F]', 'Disciplines and Occupations [H]']
| 0
| 1
| 0
| 0
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 1
| 0
|
Angiographic management of massive hemobilia due to iatrogenic trauma.
|
Ten patients with massive hemobilia in shock or preshock status were treated with angiography. The hemobilia had been induced by iatrogenic trauma: biliary drainage in seven patients, and surgery, liver biopsy, and angiography in one patient each. Angiography was performed on all patients. Embolization was performed in nine, and in the one remaining patient, spasm of the right anterior hepatic artery and catheter manipulation injured the intima and obliterated the artery. In seven patients with hepatic artery pseudoaneurysm, gelfoam particles were injected in five, however, extravasation could not be prevented in four of these patients. Permanent embolic materials were added and complete hemostatis was obtained. Hemobilia never recurred in any patient. Emergency embolization should be considered as the initial treatment of choice for hemobilia and when pseudoaneurysms are discovered, they should be obliterated by permanent embolic materials. Moreover, tumor thrombus in the portal vein is not a contraindication for this procedure.
|
['Aged', 'Angiography', 'Bile Ducts', 'Biliary Tract Surgical Procedures', 'Embolization, Therapeutic', 'Female', 'Hemobilia', 'Hepatic Artery', 'Humans', 'Iatrogenic Disease', 'Intraoperative Complications', 'Liver', 'Male', 'Middle Aged']
| 1,879,634
|
[['M01.060.116.100'], ['E01.370.350.700.060', 'E01.370.370.050'], ['A03.159.183'], ['E04.210.120'], ['E02.520.360', 'E02.926.500'], ['C23.550.414.864'], ['A07.015.114.407'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C23.550.291.875'], ['C23.550.505'], ['A03.620'], ['M01.060.116.630']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Diseases [C]', 'Organisms [B]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Histological evaluation of the effects of intraarterial chemotherapy for advanced breast cancer: a long-term followup study with respect to the survival rate.
|
To evaluate the effects of intraarterial infusion chemotherapy (IAIC) for advanced breast cancer, we examined the grade of histological responses of preoperative IAIC on tumors at the time of operation and estimated the patients' prognoses for 19 years. IAIC was done preoperatively using timely epochal anticancer drugs on 105 patients with locally advanced (Stage IIIa, IIIb) and metastatic (Stage IV) breast cancer. The survival rate of the Stage IIIb patients who showed a good histological response (Grade IIb< or =) to IAIC was 68.1% for 5 years, and 62.4% for 10 years, respectively. This was in contrast to that of the patients classified as Stage IIIb who showed a poor histological response (Grade IIa> or =) to IAIC. On the other hand, there was no significant difference in the survival rates between the Stage IIIa and IV patients with good and poor histological responses to IAIC. However, the findings showed that a good histological response to IAIC reflected a prolonged survival while the Stage IIIb and IV patients acquired a "down clinical staging" by IAIC. These results strongly suggest that IAIC thus appears to be a useful modality in the multidisciplinary treatment of advanced breast cancer, especially for Stage IIIb patients.
|
['Adult', 'Antineoplastic Agents', 'Breast Neoplasms', 'Chemotherapy, Adjuvant', 'Female', 'Follow-Up Studies', 'Humans', 'Infusions, Intra-Arterial', 'Middle Aged', 'Neoplasm Staging', 'Prognosis', 'Survival Analysis']
| 9,607,903
|
[['M01.060.116'], ['D27.505.954.248'], ['C04.588.180', 'C17.800.090.500'], ['E02.186.170', 'E02.319.170'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.319.267.510.520'], ['M01.060.116.630'], ['E01.789.625'], ['E01.789'], ['E05.318.740.998', 'N05.715.360.750.795', 'N06.850.520.830.998']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Radiosensitization by pemetrexed of human colon carcinoma cells in different cell cycle phases.
|
PURPOSE: The novel folate antimetabolite Alimta (pemetrexed disodium, LY231514) exhibits antitumor activity in a broad array of human malignancies and was recently found to enhance radiation-induced cell killing in vitro. In the present study, a possible cell cycle phase-specific radiosensitization by pemetrexed was assessed.METHODS AND MATERIALS: Widr human colon carcinoma cells were synchronized by serum withdrawal/stimulation that yielded about 80% cells with G1 DNA content 6 h after replating and more than 60% S-phase cells after 22 h, as assessed by flow cytometry. The respective cultures were irradiated with doses up to 12 Gy in combination with a subtoxic pemetrexed exposure (1.06 microM for 2 h: about 80% survival), or after mock treatment. Survival curves were generated by the clonogenic assay; apoptosis was measured by sub-G1 DNA flow cytometry.RESULTS: The combination treatment of the G1 cells and of the more radioresistant S-phase cell preparations yielded survival rates that were lower than expected for independent cell killing. Radiosensitization, calculated as the ratio of the mean inactivation doses without or with drug exposure (enhancement ratio), was not significantly different for the two cell preparations (enhancement ratio of 2.1 and 2.3, respectively) and was similar to the previously reported value for log-phase cells. Pemetrexed exposure was unable to stimulate an apoptotic response of these cells to radiation.CONCLUSIONS: Radiosensitization by pemetrexed is not cell cycle phase-specific, and the relative radioresistance of S-phase cells is retained. Apoptosis seems to have no influence on radiosensitization in this cell line.
|
['Apoptosis', 'Cell Cycle', 'Colonic Neoplasms', 'Dose-Response Relationship, Radiation', 'Glutamates', 'Guanine', 'Humans', 'Pemetrexed', 'Radiation Tolerance', 'Radiation-Sensitizing Agents', 'Tumor Cells, Cultured']
| 12,909,245
|
[['G04.146.954.035'], ['G04.144'], ['C04.588.274.476.411.307.180', 'C06.301.371.411.307.180', 'C06.405.249.411.307.180', 'C06.405.469.158.356.180', 'C06.405.469.491.307.180'], ['E05.799.513.500', 'G01.750.740.500', 'G04.712.500', 'G07.225', 'G07.738.500', 'N06.850.810.250.180'], ['D12.125.067.625', 'D12.125.119.409'], ['D03.633.100.759.758.399.454'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D03.633.100.759.758.399.454.650', 'D12.125.067.625.525', 'D12.125.119.409.525'], ['G04.712', 'G07.738'], ['D27.505.954.600'], ['A11.251.860']]
|
['Phenomena and Processes [G]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Free peroneal and its composite flap: a distant donor for head and neck reconstruction.
|
Free peroneal or its composite flap from the leg has been transferred in four patients for oro-mandibular and hypopharyngeal defects following surgery for malignancy. These flaps were successfully transplanted in all patients: two cases of free peroneal flap and two of free fibular graft with skin with no resulting problems with the leg. The peroneal osteocutaneous flap offers the following advantages: The axial vessels are preferable for microvascular surgery. Two flaps can be used with two cutaneous branches. A flap measuring up to 16 x 4.5 cm can be utilized with primary closure at the donor site. A long bone can be obtained together with skin. Surgery on the neck and harvesting of the flap can be done at the same time. The donor site is not conspicuous.
|
['Aged', 'Female', 'Fibula', 'Humans', 'Male', 'Mandible', 'Mandibular Neoplasms', 'Middle Aged', 'Pharyngeal Neoplasms', 'Pharynx', 'Surgical Flaps', 'Tomography, X-Ray Computed']
| 8,323,492
|
[['M01.060.116.100'], ['A02.835.232.043.650.321'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A02.835.232.781.324.502.632', 'A14.521.632'], ['C04.588.149.721.450.583', 'C05.116.231.754.450.583', 'C05.500.499.583', 'C05.500.607.442', 'C07.320.515.583', 'C07.320.610.583'], ['M01.060.116.630'], ['C04.588.443.665.710', 'C07.550.745', 'C09.647.710', 'C09.775.549'], ['A03.556.750', 'A04.623', 'A14.724'], ['A10.850.710', 'E07.862.710'], ['E01.370.350.350.810', 'E01.370.350.600.350.700.810', 'E01.370.350.700.700.810', 'E01.370.350.700.810.810', 'E01.370.350.825.810.810']]
|
['Named Groups [M]', 'Anatomy [A]', 'Organisms [B]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
The effects of day-to-day variability of physiological data on operator functional state classification.
|
The application of pattern classification techniques to physiological data has undergone rapid expansion. Tasks as varied as the diagnosis of disease from magnetic resonance images, brain-computer interfaces for the disabled, and the decoding of brain functioning based on electrical activity have been accomplished quite successfully with pattern classification. These classifiers have been further applied in complex cognitive tasks to improve performance, in one example as an input to adaptive automation. In order to produce generalizable results and facilitate the development of practical systems, these techniques should be stable across repeated sessions. This paper describes the application of three popular pattern classification techniques to EEG data obtained from asymptotically trained subjects performing a complex multitask across five days in one month. All three classifiers performed well above chance levels. The performance of all three was significantly negatively impacted by classifying across days; however two modifications are presented that substantially reduce misclassifications. The results demonstrate that with proper methods, pattern classification is stable enough across days and weeks to be a valid, useful approach.
|
['Electroencephalography', 'Female', 'Humans', 'Male', 'Pattern Recognition, Automated', 'Signal Processing, Computer-Assisted', 'Task Performance and Analysis', 'User-Computer Interface', 'Workload', 'Young Adult']
| 21,840,403
|
[['E01.370.376.300', 'E01.370.405.245'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['L01.399.750'], ['L01.224.800'], ['F02.784.412.846', 'F02.784.692.746', 'F02.808.600'], ['L01.224.900.910'], ['I03.946.225.500', 'N04.452.677.650.500'], ['M01.060.116.815']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Information Science [L]', 'Psychiatry and Psychology [F]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]', 'Named Groups [M]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 1
| 1
| 0
|
Application of Calcium Silicate Materials After Acid Etching May Preserve Resin-Dentin Bonds.
|
INTRODUCTION: The aim of the present study was to evaluate the effect of the application of calcium silicate materials (CSMs), after acid etching, on the longevity of the hybrid layer and marginal adaptation of composite restorations.METHODS AND MATERIALS: Eighty human permanent molars received an intrapulpal pressure of 15 cm H2O. Sixty teeth received a mesial proximal slot preparation with the gingival margin extending 1 mm below the cemento-enamel junction. The samples were divided into two groups. Group 1 received restorations using two types of etch-and-rinse adhesives: ethanol based (Single Bond, 3M ESPE, St Paul, MN, USA) and acetone based (Prime & Bond NT, Dentsply, DeTrey GmbH, Germany). In group 2 samples, a commercially available CSM (ProRoot MTA) was allowed to set before grinding and placing into a distilled water solution. This solution was applied on the cavity floor after acid etching. The surface was washed after 30 seconds followed by application of adhesives and restorations as in group 1. The samples were stored in phosphate-buffered saline for six months, maintaining the intrapulpal pressure. An epoxy replica was made, and the marginal adaptation was evaluated using scanning electron microscopy. The percentage of continuous margin (CM) was recorded for each group. Another 20 samples were used for hybrid layer evaluation. The crowns were ground to expose dentin. Intrapulpal pressure was applied. The samples were divided into two groups and restored similar to samples restored for marginal adaptation evaluation. The samples were longitudinally cut in 1-mm slices. The slices were stored under 15 cm of phosphate-buffered saline to simulate the pulpal pressure. After six months, the adhesive interface was evaluated using a scanning electron microscope. Statistical analysis was done with two-way analysis of variance with Holm-Sidak's correction for multiple comparisons.RESULTS: Application of CSMs improved the marginal adaptation values in both adhesive groups. In group 1, there were areas of incomplete penetration of resins along with evidence of partial degradation of resin tags. Samples receiving CSM application after acid etching demonstrated long and regular resin tags with very few signs of degradation.CONCLUSIONS: Application of CSMs after acid etching can be a potential avenue in preserving the resin-dentin bonds.
|
['Acid Etching, Dental', 'Calcium Compounds', 'Dental Bonding', 'Dental Marginal Adaptation', 'Dental Restoration, Permanent', 'Dentin', 'Humans', 'Microscopy, Electron, Scanning', 'Molar', 'Resins, Synthetic', 'Silicates']
| 29,953,337
|
[['E06.931.475.111'], ['D01.146'], ['E06.095'], ['E06.323.764', 'E06.658.224', 'E06.780.620'], ['E06.323.428', 'E06.780.346.737', 'E07.695.190.190'], ['A14.549.167.900.280'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.350.515.402.541', 'E05.595.402.541'], ['A14.549.167.860.525'], ['D05.750.716.822', 'D25.339.816', 'D25.720.716.822', 'J01.637.051.339.816', 'J01.637.051.720.716.822'], ['D01.578.725', 'D01.837.725.700.760']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Organisms [B]', 'Technology, Industry, and Agriculture [J]']
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Cosmetic rehabilitation following successful treatment of aggressive periodontitis.
|
UNLABELLED: With advances in periodontal therapy, many sufferers from aggressive periodontitis are retaining their teeth after successful treatment. This presents the practitioner with aesthetic and restorative challenges in these relatively young patients. Lifelong motivation is essential to the supportive therapy for these patients, and the maintenance of good aesthetics, combined with biologically acceptable corrective therapy, may help maintain a high level of motivation. Any treatment provided must naturally be conducive to maintaining long-term dental and periodontal health. This paper aims to demonstrate options for dealing with the aesthetic challenges posed by a number of patients who have undergone initial cause-related therapy for aggressive periodontitis.CLINICAL RELEVANCE: Loss of gingival tissue, tooth positional changes and tooth loss present practitioners with challenges in relation to patient satisfaction with aesthetics following advanced periodontal breakdown. A range of techniques will be required, tailored to the consequences of periodontal attachment loss, in order to satisfy patient demands.
|
['Adult', 'Aggressive Periodontitis', 'Crowns', 'Denture, Partial, Fixed', 'Esthetics, Dental', 'Female', 'Gingival Recession', 'Humans', 'Orthodontics, Corrective', 'Periodontal Prosthesis', 'Tooth Loss', 'Tooth Migration']
| 17,432,773
|
[['M01.060.116'], ['C07.465.714.533.161'], ['E06.780.346.250', 'E07.695.190.088'], ['E06.780.346.760.943.271', 'E07.695.190.200.220.220'], ['E06.420'], ['C07.465.714.258.447', 'C07.465.714.354.625'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E06.658.578'], ['E06.721.721', 'E07.695.190.610'], ['C07.465.714.804', 'C07.793.870'], ['C07.465.714.836', 'G10.549.830']]
|
['Named Groups [M]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Effects of alpha 1-adrenergic blockade on pulsatile luteinizing hormone, follicle-stimulating hormone, and prolactin secretion in polycystic ovary syndrome.
|
Central noradrenergic mechanisms may participate in the regulation of pulsatile gonadotropin secretion in women with the polycystic ovary syndrome (PCO). To examine this possibility we measured serum LH, FSH, and PRL concentrations at 10-min intervals and total testosterone and 17 beta-estradiol at 60-min intervals for 8 h basally and during the infusion of the alpha 1-adrenoceptor antagonist thymoxamine (10 micrograms/kg X min) in 10 young women with PCO. Mean and integrated serum LH concentrations as well as LH pulse frequency were not significantly altered (P = NS) during the thymoxamine infusion. However, we found an increase in LH pulse amplitude as both net (P less than 0.002) and percent (P less than 0.002) increment, as well as mean LH peak values (P less than 0.05) during alpha 1-adrenergic blockade. There were no significant changes in pulsatile FSH and PRL secretion or gonadal sex steroids during these experimental conditions. These data suggest that in PCO patients, 1) brain noradrenergic mechanisms do not play a stimulatory role in regulating the frequency of pulsatile LH secretion, 2) central noradrenergic activity inhibits LH pulse amplitude, and 3) PRL and FSH pulsatility are not altered by central noradrenergic blockade.
|
['Adolescent', 'Adrenergic alpha-Antagonists', 'Adult', 'Female', 'Follicle Stimulating Hormone', 'Humans', 'Luteinizing Hormone', 'Moxisylyte', 'Polycystic Ovary Syndrome', 'Prolactin', 'Pulsatile Flow']
| 2,889,749
|
[['M01.060.057'], ['D27.505.519.625.050.200.100', 'D27.505.696.577.050.200.100'], ['M01.060.116'], ['D06.472.699.322.576.288', 'D06.472.699.631.525.343.288', 'D12.644.548.691.525.343.288'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D06.472.699.322.576.463', 'D06.472.699.631.525.343.463', 'D12.644.548.691.525.343.463'], ['D02.092.668.387.500'], ['C04.182.612.765', 'C13.351.500.056.630.580.765', 'C19.391.630.580.765'], ['D06.472.699.322.576.773', 'D06.472.699.631.525.525', 'D12.644.548.691.525.525'], ['G01.482.620', 'G09.330.380.630.555']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Diseases [C]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
The INHERIT Model: A Tool to Jointly Improve Health, Environmental Sustainability and Health Equity through Behavior and Lifestyle Change.
|
The need for analysis and action across the interrelated domains of human behaviors and lifestyles, environmental sustainability, health and inequality is increasingly apparent. Currently, these areas are often not considered in conjunction when developing policies or interventions, introducing the potential for suboptimal or conflicting outcomes. The INHERIT model has been developed within the EU-funded project INHERIT as a tool to guide thinking and intersectoral action towards changing the behaviors and lifestyles that play such an important role in today’s multidisciplinary challenges. The model integrates ecological public health and behavioral change models, emphasizing inequalities and those parts of the causal process that are influenced by human behaviors and lifestyles. The model was developed through web-based and live discussions with experts and policy stakeholders. To test the model’s usability, the model was applied to aspects of food consumption. This paper shows that the INHERIT model can serve as a tool to identify opportunities for change in important −food-related behaviors and lifestyles and to examine how they impact on health, health inequalities, and the environment in Europe and beyond. The INHERIT model helps clarify these interrelated domains, creating new opportunities to improve environmental health and health inequality, while taking our planetary boundaries into consideration.
|
['Conservation of Natural Resources', 'Delivery of Health Care', 'Europe', 'Health Behavior', 'Health Equity', 'Health Promotion', 'Health Status Disparities', 'Humans', 'Life Style', 'Public Health']
| 29,986,493
|
[['J01.256', 'N06.230.080'], ['N04.590.374', 'N05.300'], ['Z01.542'], ['F01.145.488'], ['N04.590.374.350.500', 'N05.300.430.383'], ['I02.233.332.445', 'N02.421.726.407.579'], ['I01.240.425.675', 'N01.224.425.437', 'N06.850.505.400.425.675'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F01.829.458'], ['H02.403.720', 'N01.400.550', 'N06.850']]
|
['Technology, Industry, and Agriculture [J]', 'Health Care [N]', 'Geographicals [Z]', 'Psychiatry and Psychology [F]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Organisms [B]', 'Disciplines and Occupations [H]']
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 1
| 1
| 1
| 0
| 0
| 1
| 1
|
Altered expression of p53 and p27 proteins, alone or combined, as a predictor of metastatic potential in early invasive carcinoma of colon and rectum--a comparative clinicopathologic and molecular analysis.
|
To place the choice of therapy (endoscopic resection or radical surgery) in early invasive carcinoma (EIC) of colon and rectum on a more rational basis, this study sought to identify molecular predictors of metastasis. Several morphologic risk factors (histologic type, degree of tumor invasion, lymphatic and venous invasion) and expression of p53 and p27 proteins in the primary tumor were compared in 80 patients with EIC, including 12 (15%) with metastasis or recurrence (or both). Of the factors enumerated, deeper invasion of the submucosal layer, lymphatic-venous invasion, p53 overexpression, and decreased expression of p27 were correlated significantly with metastasis. The results also indicated that altered expression of p53 or p27 is independently relevant to metastasis of EIC. Analysis of these markers, together with determination of the morphologic risk factors, could complement the identification of patients with metastasis on the basis of known morphologic risk factors. Because the molecular factors can be assessed more objectively than can the morphologic parameters, they may strengthen the ability to identify EIC that has undergone, or will undergo, metastasis.
|
['Adenocarcinoma', 'Adult', 'Aged', 'Aged, 80 and over', 'Biomarkers, Tumor', 'Cell Cycle Proteins', 'Chi-Square Distribution', 'Colorectal Neoplasms', 'Cyclin-Dependent Kinase Inhibitor p27', 'Cyclin-Dependent Kinases', 'Enzyme Inhibitors', 'Female', 'Gene Expression Regulation, Neoplastic', 'Genes, Tumor Suppressor', 'Humans', 'Male', 'Microtubule-Associated Proteins', 'Middle Aged', 'Neoplasm Invasiveness', 'Neoplasm Metastasis', 'Neoplasm Proteins', 'Prognosis', 'Risk Factors', 'Statistics, Nonparametric', 'Tumor Suppressor Protein p53', 'Tumor Suppressor Proteins']
| 11,059,565
|
[['C04.557.470.200.025'], ['M01.060.116'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['D23.101.140'], ['D12.776.167'], ['E05.318.740.994.300', 'G17.820.300', 'N05.715.360.750.750.200', 'N06.850.520.830.994.300'], ['C04.588.274.476.411.307', 'C06.301.371.411.307', 'C06.405.249.411.307', 'C06.405.469.158.356', 'C06.405.469.491.307', 'C06.405.469.860.180'], ['D12.644.360.225.600', 'D12.776.167.187.600', 'D12.776.476.225.600', 'D12.776.624.776.355.600'], ['D08.811.913.696.620.682.700.646.500', 'D12.644.360.250', 'D12.776.167.200', 'D12.776.476.250'], ['D27.505.519.389'], ['G05.308.370'], ['G05.360.340.024.340.375.249', 'G05.360.340.024.340.415.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.776.220.600.450', 'D12.776.631.560'], ['M01.060.116.630'], ['C04.697.645', 'C23.550.727.645'], ['C04.697.650', 'C23.550.727.650'], ['D12.776.624'], ['E01.789'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['E05.318.740.995', 'N05.715.360.750.760', 'N06.850.520.830.995'], ['D12.776.157.687.650', 'D12.776.260.820', 'D12.776.624.776.775', 'D12.776.660.720.650', 'D12.776.744.845'], ['D12.776.624.776']]
|
['Diseases [C]', 'Named Groups [M]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Genome sequence of Desulfitobacterium hafniense DCB-2, a Gram-positive anaerobe capable of dehalogenation and metal reduction.
|
BACKGROUND: The genome of the Gram-positive, metal-reducing, dehalorespiring Desulfitobacterium hafniense DCB-2 was sequenced in order to gain insights into its metabolic capacities, adaptive physiology, and regulatory machineries, and to compare with that of Desulfitobacterium hafniense Y51, the phylogenetically closest strain among the species with a sequenced genome.RESULTS: The genome of Desulfitobacterium hafniense DCB-2 is composed of a 5,279,134-bp circular chromosome with 5,042 predicted genes. Genome content and parallel physiological studies support the cell's ability to fix N2 and CO2, form spores and biofilms, reduce metals, and use a variety of electron acceptors in respiration, including halogenated organic compounds. The genome contained seven reductive dehalogenase genes and four nitrogenase gene homologs but lacked the Nar respiratory nitrate reductase system. The D. hafniense DCB-2 genome contained genes for 43 RNA polymerase sigma factors including 27 sigma-24 subunits, 59 two-component signal transduction systems, and about 730 transporter proteins. In addition, it contained genes for 53 molybdopterin-binding oxidoreductases, 19 flavoprotein paralogs of the fumarate reductase, and many other FAD/FMN-binding oxidoreductases, proving the cell's versatility in both adaptive and reductive capacities. Together with the ability to form spores, the presence of the CO2-fixing Wood-Ljungdahl pathway and the genes associated with oxygen tolerance add flexibility to the cell's options for survival under stress.CONCLUSIONS: D. hafniense DCB-2's genome contains genes consistent with its abilities for dehalogenation, metal reduction, N2 and CO2 fixation, anaerobic respiration, oxygen tolerance, spore formation, and biofilm formation which make this organism a potential candidate for bioremediation at contaminated sites.
|
['Carbon Dioxide', 'DNA, Bacterial', 'Desulfitobacterium', 'Genes, Bacterial', 'Genome, Bacterial', 'Halogens', 'Metabolic Networks and Pathways', 'Metals', 'Molecular Sequence Data', 'Nitrogen Fixation', 'Organic Chemicals', 'Oxidation-Reduction', 'Sequence Analysis, DNA']
| 22,316,246
|
[['D01.200.200', 'D01.362.150', 'D01.650.550.200'], ['D13.444.308.212'], ['B03.353.625.782.149', 'B03.900.174'], ['G05.360.340.024.340.364.249', 'G05.360.340.358.024.249', 'G05.360.340.358.207.249'], ['G05.360.340.358.207'], ['D01.268.380'], ['G03.493'], ['D01.552'], ['L01.453.245.667'], ['G02.111.071.630', 'G02.111.587.750', 'G02.607.560.750', 'G03.087.630', 'G06.625', 'G16.500.768.600'], ['D02'], ['G02.700', 'G03.295.531'], ['E05.393.760.700']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Changes in prejunctional potency of B-HT 933 during aging in the rat vas deferens.
|
1. Age-related changes in prejunctional alpha 2-adrenoceptors were examined in the rat vas deferens using pharmacological techniques. 2. B-HT 933 (1 x 10(-8) - 1 x 10(-6) mol/L) caused a concentration-dependent inhibition of isometric contractions (tetrodotoxin-sensitive) induced by stimulation with single field-stimulus pulses, in both the epididymal and prostatic regions of rat vas deferens. The concentration-response curve to B-HT 933 was shifted to the right with age in the prostatic regions of the vas deferens. 3. In high concentrations (10(-6) - 3 x 10(-4) mol/L), B-HT 933 caused concentration-dependent enhancement of the contractile response to stimulation and evoked spontaneous contractile activity. No significant difference in this postjunctional activity occurred with age in either the prostatic or epididymal regions of the vas deferens. 4. Schild analysis revealed no significant differences in pA2 values for the antagonisms of the prejunctional inhibitory effect of B-HT 933 by rauwolscine in either the prostatic or epididymal regions of vas deferens between young and old rats. 5. These results could be interpreted as a decrease in alpha 2-adrenoceptor number with age. The more marked decrease in the prejunctional inhibitor potency of B-HT 933 in prostatic regions of vas deferens with aging may be due to a smaller receptor reserve in this region of the vas deferens.
|
['Aging', 'Animals', 'Azepines', 'Male', 'Muscle Contraction', 'Rats', 'Rats, Inbred Strains', 'Receptors, Adrenergic, alpha', 'Vas Deferens', 'Yohimbine']
| 2,170,068
|
[['G07.345.124'], ['B01.050'], ['D03.383.066'], ['G11.427.494'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.050.199.520.760', 'B01.050.150.900.649.313.992.635.505.700.400'], ['D12.776.543.750.670.300.300.300', 'D12.776.543.750.695.150.300.300', 'D12.776.543.750.720.330.300.300'], ['A05.360.444.930'], ['D03.132.436.681.933', 'D03.633.100.473.402.681.933', 'D03.633.100.496.500.500.681.933']]
|
['Phenomena and Processes [G]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Motor mechanisms associated with slowing of the gastric emptying of a solid meal by an intraduodenal lipid infusion.
|
The aim of this study was to define better the motor phenomena associated with the slowing of gastric emptying by a duodenal lipid infusion. Antral, pyloric and duodenal motility were recorded in 10 healthy subjects with a manometric assembly which incorporated multiple perfused side-holes and a sleeve sensor positioned astride the pylorus. The gastric emptying of a standard solid meal and the distribution of the ingesta between the proximal and distal stomach were monitored with a radionuclide technique. A triglyceride emulsion was infused into the duodenum for 45 min once 25% of the meal had emptied. The infusion caused significant slowing in the rate of gastric emptying (P less than 0.01). This slowing in gastric emptying was associated with the suppression of pressure waves in the distal antrum (P less than 0.01) and proximal duodenum (P less than 0.01), the induction of pressure waves isolated to a narrow pyloric segment (P less than 0.01), and a redistribution of ingesta from the distal to proximal stomach. These findings suggest that pressure waves isolated to the pylorus, changes in the intragastric distribution of ingested food, and changes in proximal duodenal motility may all act in concert with changes in antral motility to regulate the gastric emptying of solids.
|
['Adult', 'Duodenum', 'Fat Emulsions, Intravenous', 'Female', 'Food', 'Gastric Emptying', 'Gastrointestinal Motility', 'Humans', 'Hydrogen-Ion Concentration', 'Male', 'Manometry', 'Pylorus', 'Technetium Tc 99m Sulfur Colloid']
| 2,491,209
|
[['M01.060.116'], ['A03.556.124.684.124', 'A03.556.875.249'], ['D26.255.165.260.270', 'D26.776.708.733.500', 'D27.505.954.578.733.500', 'D27.720.752.733.500'], ['G07.203.300', 'J02.500'], ['G10.261.360.400'], ['G10.261.360'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G02.300'], ['E05.559'], ['A03.556.875.875.799'], ['D01.875.900', 'D01.925.950']]
|
['Named Groups [M]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Technology, Industry, and Agriculture [J]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
|
[Study on changes in extravascular lung water during early postoperative periods in thoracic esophageal cancer--with special emphasis on their relation to postoperative renal function].
|
Twenty-seven patients with esophageal cancer received an intrathoracic esophagectomy, lymphadenectomy and esophageal reconstruction performed in one stage. They were analyzed for respiratory and hemodynamic function parameters and also observed for the time course of extravascular lung water (EVLW), water balance, renal function as well as colloid osmotic pressure (COP) of plasma. And they were clarified the pathogenetic mechanism of post-operative pulmonary complications mainly from the aspects of pathophysiology of pulmonary edema and functional interrelationship of organs. Two groups of patients, i.e. those undergoing extended lymphadenectomy (particularly for lymph nodes of both sides of neck and upper mediastinum) and those of old age (70 years or above), were investigated for eventual characteristic features of postoperative changes in the parameters mentioned above. In the group of patients with postoperative pulmonary complications, a significant negative correlation was noted to exist between the plasma colloid osmotic pressure-pulmonary artery wedge pressure (COP-PAW) gradient and EVLW and between the former parameter and postoperative renal function (p less than 0.01). A postoperative lowering of renal function observed in the group with postoperative pulmonary complications was due mainly to depressed left ventricular function immediately following operation and assumed to play a significant role in the production of pulmonary edema as a hydrostatic factor subject to the Starling's low. In the group undergoing extended lymphadenectomy, extensive lymph node dissection reduced plasma colloid osmotic pressure. This reduction was thought to bring about a diminution of COP-PAW gradient, produce a transient depression of left ventricular function and augmentation of pulmonary edema, and to stimulate the formation of intrapulmonary shunting. In the old age group, their and renal function depressed immediately after operation because of advanced age. And for the maintenance of cardiac function massive water intake was required. They led to retention of water and thereby played a direct role in the causation of increase in EVLW and in intrapulmonary shunt. All these observations point to the necessity of initiating carefully planned management early in the postoperative period that takes these pathophysiologic features well into account.
|
['Aged', 'Esophageal Neoplasms', 'Extravascular Lung Water', 'Female', 'Humans', 'Kidney', 'Male', 'Middle Aged', 'Postoperative Period', 'Time Factors']
| 2,348,127
|
[['M01.060.116.100'], ['C04.588.274.476.205', 'C04.588.443.353', 'C06.301.371.205', 'C06.405.117.430', 'C06.405.249.205'], ['A12.207.270.300'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A05.810.453'], ['M01.060.116.630'], ['E04.614.750', 'N02.421.585.753.750'], ['G01.910.857']]
|
['Named Groups [M]', 'Diseases [C]', 'Anatomy [A]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
A proteasome inhibitor confers cardioprotection.
|
OBJECTIVE: In several cell types, proteasome inhibitors like carbobenzoxyl-leucinyl-leucinyl-leucinal (MG132) induce the 72 kDa heat shock protein (Hsp72) and exert cell protective effects. However, data in cardiomyocytes are currently lacking.METHODS AND RESULTS: We investigated the effects of MG132 in cultured neonatal rat cardiomyocytes. MG132 time- and concentration-dependently induced Hsp72 and Hsp32 at mRNA and protein levels. Although Hsp60 mRNA was induced, Hsp60 protein levels were not altered. MG132 activated p38 MAP kinase already after 0.5 h. Hsp mRNA induction started after 2 h of MG132 treatment. Subsequently, Hsp72 and Hsp32 protein levels were increased after 4 h. SB202190, an inhibitor of p38 MAP kinase, concentration-dependently attenuated MG132-induced Hsp72-and Hsp32-elevations (by 59% and 41%, respectively, at 1 microM SB202190). In contrast, herbimycin A, a known inductor of Hsp72 in cardiomyocytes, enhanced the MG132-induced Hsp72 and Hsp32 expression even further: additionally applied 2 microM herbimycin A induced Hsp72 and Hsp32 about 2-fold higher than 1 microM MG132 alone. MG132 (1 microM) decreased the hyperthermia- or hydrogen peroxide-induced release of lactate dehydrogenase by 45% and by 35%, respectively (P<0.05, n=5). MG132 (1 microM) prolonged the spontaneous beating time of cardiomyocytes at 46 degrees C from 5+/-2 min (control hyperthermia) to 28+/-5 min (P<0.05, n=4). Thus, inhibition of the proteasome function by MG132 protects cardiomyocytes against hyperthermic or oxidative injury. This protective effect and Hsp induction were abolished by 1 microM SB202190.CONCLUSION: Proteasome inhibition results in p38 MAP kinase-dependent induction of Hsp72 and Hsp32 and might be a novel cardioprotective modality.
|
['Animals', 'Benzoquinones', 'Blotting, Western', 'Cells, Cultured', 'Cysteine Proteinase Inhibitors', 'Dose-Response Relationship, Drug', 'Enzyme Inhibitors', 'Fever', 'HSP72 Heat-Shock Proteins', 'Heat-Shock Proteins', 'Heme Oxygenase (Decyclizing)', 'Heme Oxygenase-1', 'Imidazoles', 'In Vitro Techniques', 'Lactams, Macrocyclic', 'Leupeptins', 'Mitogen-Activated Protein Kinases', 'Myocardium', 'Oxidative Stress', 'Papillary Muscles', 'Pyridines', 'Quinones', 'RNA, Messenger', 'Rats', 'Rats, Sprague-Dawley', 'Rifabutin', 'p38 Mitogen-Activated Protein Kinases']
| 12,062,370
|
[['B01.050'], ['D02.806.250'], ['E05.196.401.143', 'E05.301.300.096', 'E05.478.566.320.200', 'E05.601.262', 'E05.601.470.320.200'], ['A11.251'], ['D27.505.519.389.745.325'], ['G07.690.773.875', 'G07.690.936.500'], ['D27.505.519.389'], ['C23.888.119.344'], ['D12.776.580.216.375.202'], ['D12.776.580.216'], ['D08.811.682.690.708.410'], ['D08.811.682.690.708.410.500'], ['D03.383.129.308'], ['E05.481'], ['D02.065.589.327', 'D04.345.295'], ['D12.644.456.580'], ['D08.811.913.696.620.682.700.567', 'D12.644.360.450', 'D12.776.476.450'], ['A02.633.580', 'A07.541.704', 'A10.690.552.750'], ['G03.673', 'G07.775.750'], ['A02.633.580.680', 'A07.541.510.619', 'A07.541.704.750'], ['D03.383.725'], ['D02.806'], ['D13.444.735.544'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.750'], ['D03.633.400.811.650', 'D04.345.295.750.650'], ['D08.811.913.696.620.682.700.567.843', 'D12.644.360.450.835', 'D12.776.476.450.835']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
"There are more important things to worry about": attitudes and behaviours towards leisure noise and use of hearing protection in young adults.
|
OBJECTIVE: Noise-induced hearing problems among young adults are increasing due to participation in loud activities. This study explored attitudes towards leisure noise, hearing protection, and perceived susceptibility to noise damage in young adults with no diagnosed hearing problems. Understanding attitudes and behaviours will assist with the future development of strategies to improve awareness and use of hearing protection.DESIGN: A qualitative study.STUDY SAMPLE: Four focus groups, with 28 adults aged 18-35 years (6 male; 22 female; mean age 23 years).RESULTS: Using framework analysis, five themes emerged. Earplug use occurred when participants had experienced previous temporary hearing damage (i.e. short-lived tinnitus or hearing loss). Others chose not to use earplugs because music venues are expected to be loud. Peer behaviours and opinions also had a strong influence over earplug use. A lack of knowledge of hearing-related damage resulted in a lack of concern for hearing health and other health conditions taking priority.CONCLUSIONS: The challenge is to present hearing health messages that are relevant and accessible to young adults. Music and entertainment venues must also take greater responsibility to protect the hearing of its customers by at least informing visitors of the dangers of loud music.
|
['Adolescent', 'Adult', 'Attitude to Health', 'Ear Protective Devices', 'Female', 'Focus Groups', 'Hearing Loss, Noise-Induced', 'Humans', 'Leisure Activities', 'Male', 'Noise', 'Qualitative Research', 'Young Adult']
| 29,378,448
|
[['M01.060.057'], ['M01.060.116'], ['F01.100.150', 'N05.300.150'], ['E07.700.250', 'J01.637.215.600.199', 'J01.637.708.560.250'], ['E05.318.308.112', 'N05.715.360.300.269', 'N06.850.520.308.112'], ['C09.218.458.341.887.460', 'C10.597.751.418.341.887.460', 'C23.888.592.763.393.341.887.460'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['I03.450'], ['G01.750.770.776.567', 'G16.500.275.600', 'N06.230.400', 'N06.850.460.610'], ['H01.770.644.241.850'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Psychiatry and Psychology [F]', 'Health Care [N]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Technology, Industry, and Agriculture [J]', 'Diseases [C]', 'Organisms [B]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Phenomena and Processes [G]', 'Disciplines and Occupations [H]']
| 0
| 1
| 1
| 0
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
|
Concurrent chemoradiotherapy with docetaxel, cisplatin and 5-fluorouracil (TPF) in patients with locally advanced squamous cell carcinoma of the head and neck.
|
OBJECTIVE: Compared with radiotherapy alone, concurrent chemoradiotherapy significantly improves survival rates for patients with squamous cell carcinoma of the head and neck. The aim of this study was to retrospectively evaluate the efficacy, toxicity and long-term prognosis of concurrent chemoradiotherapy with docetaxel, cisplatin and 5-fluorouracil chemotherapy.METHODS: A total of 140 patients were enrolled and evaluated. Patients were received two cycles of docetaxel, cisplatin and 5-fluorouracil chemotherapy (docetaxel [50 mg/m(2): Day 1], cisplatin [60 mg/m(2): Day 4] and continuous 5-fluorouracil [600 mg/m(2)/day: Days 1-5]) during definitive radiotherapy.RESULTS: The overall response rate was 97.1%. The 3 and 5-year overall survival rates were 83.3 and 79.2%, respectively. The 3 and 5-year disease-specific survival rates were 84.2 and 80.0%, respectively. Among patients with laryngeal or hypopharyngeal carcinoma, the 5-year laryngectomy-free survival rate was 64.9%.CONCLUSIONS: Concurrent chemoradiotherapy with docetaxel, cisplatin and 5-fluorouracil showed excellent survival and organ preservation rates for the patients with locally advanced squamous cell carcinoma of the head and neck.
|
['Adult', 'Aged', 'Antineoplastic Combined Chemotherapy Protocols', 'Carcinoma, Squamous Cell', 'Chemoradiotherapy', 'Cisplatin', 'Disease-Free Survival', 'Docetaxel', 'Drug Administration Schedule', 'Female', 'Fluorouracil', 'Head and Neck Neoplasms', 'Humans', 'Japan', 'Kaplan-Meier Estimate', 'Male', 'Middle Aged', 'Retrospective Studies', 'Taxoids', 'Treatment Outcome']
| 24,688,084
|
[['M01.060.116'], ['M01.060.116.100'], ['E02.183.750.500', 'E02.319.077.500', 'E02.319.310.037'], ['C04.557.470.200.400', 'C04.557.470.700.400'], ['E02.186.079', 'E02.319.164', 'E02.815.160'], ['D01.210.375', 'D01.625.125', 'D01.710.100'], ['E01.789.800.190', 'E05.318.740.998.300', 'N04.761.559.590.800.190', 'N05.715.360.575.575.800.190', 'N05.715.360.750.795.300', 'N06.850.520.830.998.300'], ['D02.455.426.392.368.242.888.389', 'D02.455.849.291.850.389'], ['E02.319.283'], ['D03.383.742.698.875.404'], ['C04.588.443'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['Z01.252.474.463', 'Z01.639.595'], ['E05.318.740.998.650', 'N05.715.360.750.795.650', 'N06.850.520.830.998.650'], ['M01.060.116.630'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['D02.455.426.392.368.242.888', 'D02.455.849.291.850'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Health Care [N]', 'Organisms [B]', 'Geographicals [Z]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
The analysis of incomplete data in the three-period two-treatment cross-over design for clinical trials.
|
The additional time to complete a three-period two-treatment (3P2T) cross-over trial may cause a greater number of patient dropouts than with a two-period trial. This paper develops maximum likelihood (ML), single imputation and multiple imputation missing data analysis methods for the 3P2T cross-over designs. We use a simulation study to compare and contrast these methods with one another and with the benchmark method of missing data analysis for cross-over trials, the complete case (CC) method. Data patterns examined include those where the missingness differs between the drug types and depends on the unobserved data. Depending on the missing data mechanism and the rate of missingness of the data, one can realize substantial improvements in information recovery by using data from the partially completed patients. We recommend these approaches for the 3P2T cross-over designs.
|
['Analysis of Variance', 'Bias', 'Clinical Trials as Topic', 'Cross-Over Studies', 'Data Interpretation, Statistical', 'Effect Modifier, Epidemiologic', 'Humans', 'Likelihood Functions', 'Reproducibility of Results']
| 8,614,750
|
[['E05.318.740.150', 'N05.715.360.750.125', 'N06.850.520.830.150'], ['N05.715.350.150', 'N06.850.490.500'], ['E05.318.372.250.250', 'N05.715.360.330.250.250', 'N06.850.520.450.250.250'], ['E05.318.370.150', 'N05.715.360.325.150', 'N06.850.520.445.150'], ['E05.245.380', 'E05.318.740.300', 'L01.313.500.750.190.380', 'N05.715.360.750.300', 'N06.850.520.830.300'], ['N05.715.350.350', 'N06.850.490.734'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.740.500.475', 'E05.318.740.600.400', 'E05.599.835.500', 'N05.715.360.750.530.450', 'N05.715.360.750.625.450', 'N06.850.520.830.500.475', 'N06.850.520.830.600.400'], ['E05.318.370.725', 'E05.337.851', 'N05.715.360.325.685', 'N06.850.520.445.725']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Information Science [L]', 'Organisms [B]']
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 1
| 0
|
[Long-term results of the early endoscopic treatment of acquired tracheal-subglottic stenosis: 10 years of experience].
|
INTRODUCTION: Acquired stenosis of the airway is a common complication after endotracheal intubation. Endoscopic dilation has been accepted as the treatment of choice in cases detected precociously. Our goal is to know the current status of the patients treated in our hospital with endoscopic dilation in the last 10 years.MATERIAL AND METHODS: Retrospective cohort study of patients with subglottic and tracheal acquired stenosis (STAS) early treated endoscopically with balloon dilation at our center in the last 10 years. Bronchoscopy control at 2 weeks, a month, 3 and 6 months post-dilation were performed and later on depending on the symptoms.RESULTS: 32 patient were treated in the period considered. The median age was 4.5 (3-120) months. There were necessary 2.5 (1-5) dilations per patient. All cases were extubated in the operating room or in the following 24 hours. There were no complications during the procedure. Follow-up time was 6 (1-10) years. Only 1 of the 32 patients have had recurrence of stenosis 2 years after, it was secondary to reintubations due to new surgical interventions; which it was dilated successfully.CONCLUSIONS: Early endoscopic dilation in the acquired airway stenosis is a safe and effective long-term procedure. The results support the use of this technique as a treatment of choice in these patients.
|
['Bronchoscopy', 'Child', 'Child, Preschool', 'Cohort Studies', 'Dilatation', 'Endoscopy', 'Female', 'Follow-Up Studies', 'Humans', 'Infant', 'Intubation, Intratracheal', 'Laryngostenosis', 'Male', 'Retrospective Studies', 'Time Factors', 'Tracheal Stenosis', 'Treatment Outcome']
| 29,419,952
|
[['E01.370.386.105', 'E01.370.388.250.100', 'E04.502.250.100', 'E04.928.600.080'], ['M01.060.406'], ['M01.060.406.448'], ['E05.318.372.500.750', 'N05.715.360.330.500.750', 'N06.850.520.450.500.750'], ['E05.284'], ['E01.370.388.250', 'E04.502.250'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703'], ['E02.041.500', 'E02.585.578', 'E05.497.578'], ['C08.360.591', 'C09.400.591', 'C16.131.740.658'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['G01.910.857'], ['C08.907.663'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Named Groups [M]', 'Health Care [N]', 'Organisms [B]', 'Diseases [C]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Accelerated progression from islet autoimmunity to diabetes is causing the escalating incidence of type 1 diabetes in young children.
|
The incidence of type 1 diabetes is rising worldwide, particularly in young children. Since type 1 diabetes is preceded by autoimmunity to islet antigens, there must be a consequent increase in the incidence of islet autoimmunity in young children or a more rapid rate of progression to diabetes once islet autoimmunity initiates. This study was to determine whether the incidence of islet autoimmunity or the rate of progression from autoimmunity to diabetes onset has changed over a 20-year period in children genetically predisposed to type 1 diabetes. Between 1989 and 2010, children who were first-degree relatives of patients with type 1 diabetes and who were born in Germany were prospectively followed from birth without intervention. A total of 324 children (BABYDIAB study) born between 1989 and 2000 and 216 children (TEDDY study) born between 2004 and 2010 with matched HLA genotypes were recruited before age 3 months and included for analysis. Children were followed for the development of autoantibodies to insulin, GAD, and IA-2, and for progression to diabetes. The cumulative frequency of diabetes by age 4 years was 2.5% (95% CI 0.8-4.2%) in BABYDIAB children and 6.2% (95% CI 2.3-10.1%) in TEDDY children (p = 0.03). The cumulative frequency of islet autoantibodies by age 4 years was similar in the children from both studies (11.3% vs 13.9%). Progression to diabetes from the development of islet autoantibodies was markedly increased in autoantibody-positive children from the more recently recruited TEDDY cohort (50% progression within 85.2 months for BABYDIAB children vs 9.6 months for TEDDY children; p = 0.009), also if children were further selected on the basis of high-risk HLA genotypes or the development of autoantibodies to multiple islet antigens (p = 0.01). The findings suggest that recent increasing incidence of type 1 diabetes in young children could be due to weakening of mechanisms that normally regulate autoimmune destruction of islet beta cells.
|
['Autoantibodies', 'Autoimmunity', 'Child', 'Diabetes Mellitus, Type 1', 'Disease Progression', 'Genetic Predisposition to Disease', 'HLA-DQ Antigens', 'HLA-DR Antigens', 'Humans', 'Incidence', 'Islets of Langerhans']
| 21,376,535
|
[['D12.776.124.486.485.114.323', 'D12.776.124.790.651.114.323', 'D12.776.377.715.548.114.323'], ['G12.450.192'], ['M01.060.406'], ['C18.452.394.750.124', 'C19.246.267', 'C20.111.327'], ['C23.550.291.656'], ['C23.550.291.687.500', 'G05.380.355'], ['D12.776.395.550.509.400.430', 'D12.776.543.550.440.400.430', 'D23.050.301.500.400.400.430', 'D23.050.301.500.450.400.430', 'D23.050.705.552.410.400.430', 'D23.050.705.552.450.400.430'], ['D12.776.395.550.509.400.440', 'D12.776.543.550.440.400.440', 'D23.050.301.500.400.400.440', 'D23.050.301.500.450.400.440', 'D23.050.705.552.410.400.440', 'D23.050.705.552.450.400.440'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.308.985.525.375', 'N01.224.935.597.500', 'N06.850.505.400.975.525.375', 'N06.850.520.308.985.525.375'], ['A03.734.414', 'A06.300.414']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Named Groups [M]', 'Diseases [C]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
An unambiguous structural elucidation of a 1,3-beta-D-glucan obtained from liquid-cultured Grifola frondosa by solution NMR experiments.
|
Grifolan LE (GRN-LE), a purified beta-D-glucan, which is obtained from liquid-cultured Grifola frondosa, exhibits various biological activities, including antitumor effects. Significant progress has been made in the study of these effects. However, an unambiguous structural characterization of GRN-LE using NMR spectroscopy has not been carried out as yet. It is well accepted that the biological effects of a beta-glucan depend on its primary structure, conformation, and molecular weight. In the present study, we unambiguously elucidate the primary structure of GRN-LE using NMR spectroscopy. The data presented here reveal that GRN-LE comprises a 1,3-beta-D-glucan backbone with a single 1,6-beta-D-glucosyl side branching unit on every third residue.
|
['Cells, Cultured', 'Glucans', 'Grifola', 'Ligands', 'Magnetic Resonance Spectroscopy', 'Solutions', 'beta-Glucans']
| 19,084,824
|
[['A11.251'], ['D05.750.078.562', 'D09.698.365'], ['B01.300.179.120.372'], ['D27.720.470.480'], ['E05.196.867.519'], ['D26.776'], ['D09.698.365.089']]
|
['Anatomy [A]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Genotyping the hemophilia inversion hotspot by use of inverse PCR.
|
BACKGROUND: Factor VIII intron 22 inversions (Inv22) cause 40%-45% of severe cases of hemophilia A in all human populations. Currently, Inv22 can be analyzed either by Southern blotting or by rapid long-distance-PCR-based approaches. We describe an alternative method using inverse-PCR (I-PCR).METHODS: I-PCR involved 3 steps: (a) BclI restriction; (b) self-ligation of restriction fragments, providing BclI rings; and (c) standard multiplex-PCR analysis. PCR was achieved by use of a set of 3 primers that yielded a 487-bp amplicon for the nonrearranged intragenic allele and a 559-bp amplicon for the Inv22 allele. Specific primer sites were targeted by masking relevant regions for human repeats and low-complexity DNA. Inv22 I-PCR was applied to samples from 16 individuals (8 women and 8 men) representing 24 X chromosomes previously genotyped by Southern blotting. Additionally, we evaluated the sensitivity and the ability to assess eventual Inv22 carrier mosaicisms by experiments using artificial DNA mixtures (Inv22 + no-Inv22 male samples).RESULTS: Results for previously genotyped samples agreed with results of Southern blot analyses. As expected, cell composition of the artificial mosaic was linearly reflected by the relative intensities of Inv22 signals. I-PCR was estimated to detect Inv22-positive cells at concentrations as low as approximately 5%.CONCLUSION: The proposed technique provides a rapid tool for Inv22 genotyping.
|
['Blotting, Southern', 'Chromosome Inversion', 'Chromosomes, Human', 'Factor VIII', 'Female', 'Genotype', 'Hemophilia A', 'Heterozygote', 'Humans', 'Introns', 'Male', 'Polymerase Chain Reaction']
| 15,860,568
|
[['E05.196.401.114', 'E05.301.300.087', 'E05.601.150'], ['C23.550.210.190', 'G05.365.590.175.190', 'G05.365.590.770.500', 'G05.558.805.500'], ['A11.284.187.520.300', 'G05.360.162.520.300'], ['D12.776.124.125.350', 'D12.776.811.286', 'D23.119.350'], ['G05.380'], ['C15.378.100.100.500', 'C15.378.100.141.500', 'C15.378.463.500', 'C16.320.099.500'], ['G05.380.383'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G05.360.340.024.220.400', 'G05.360.340.024.340.137.515'], ['E05.393.620.500']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Organisms [B]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[Aggressive angiomyxoma of the vulva and perineum].
|
Reported in this paper are two cases of aggressive perineovulvar angiomyxoma. Soft, painless tumours with gelatinous cut surface were detected in either patient, two women aged 32 and 46 years. Histological findings included fibromyxoid stroma with spindle-shaped and stellate cells as well as vascularization in striking abundance. Most of the vessels were thin-walled. There was no plexiform arborization. Strongly atypical nuclei or mitoses or necrotic foci were not recordable. The tumour exhibited infiltrative growth. Tumour cells could be immunohistochemically associated with antibodies against vimentin, whereas negative responses were recorded from antibodies against S-100 protein, factor-VIII-associated protein and pancytokeratin. Aggressive angiomyxoma is a biologically benign neoplasia prone to recurrence and typically localized in soft tissue of the pelvic region. Metastases so far have never been found. Wide local excision has proved to be the optional therapeutic approach.
|
['Adult', 'Female', 'Humans', 'Immunohistochemistry', 'Middle Aged', 'Myxoma', 'Perineum', 'Vimentin', 'Vulvar Neoplasms']
| 1,420,111
|
[['M01.060.116'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.225.500.607.512', 'E01.370.225.750.551.512', 'E05.200.500.607.512', 'E05.200.750.551.512', 'E05.478.583', 'H01.158.100.656.234.512', 'H01.158.201.344.512', 'H01.158.201.486.512', 'H01.181.122.573.512', 'H01.181.122.605.512'], ['M01.060.116.630'], ['C04.557.450.565.550'], ['A01.719'], ['D05.750.078.593.900', 'D12.776.220.475.900'], ['C04.588.945.418.968', 'C13.351.500.944.819', 'C13.351.937.418.968']]
|
['Named Groups [M]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Diseases [C]', 'Anatomy [A]', 'Chemicals and Drugs [D]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
|
Follicular centre cell lymphoma with alpha heavy chain disease. A histopathological and immunohistological study.
|
The first recorded case of a small intestinal lymphoma with alpha heavy chain disease occurring in Hungary is reported. The clinical manifestation of the disease and the focal distribution of mucosal alterations do not fulfill the criteria to make a diagnosis of Mediterranean type lymphoma (MTL) but the lymphomatous segments of the jejunum show the same pathological and immunohistological characteristics as seen in MTL. One of the basic features of MTL, the so called lympho-histiocytic nodules, which have been suspected by previous authors to represent an incipient neoplastic process involving histiocytic cells, is identified as follicular centroblastic/centrocytic malignant lymphoma. The cytogenetical connection between the massive proliferation of abnormal alpha chain producing plasma cells and neoplastic germinal centres is substantiated by direct immunohistological evidence using a combined immunofluorescent and immunoperoxidase technique to detect heavy and light chains within the same cell. The sarcomatous-appearing pleomorphic cell proliferation is interpreted as an anaplastic change in the centroblastic/centrocytic lymphoma. Unequivocal evidence for an abnormal IgA production in this pleomorphic component has not been obtained. Our observations suggest that in alpha heavy chain disease the neoplastic cell population originates in the germinal centres.
|
['Heavy Chain Disease', 'Humans', 'Immunoglobulin alpha-Chains', 'Intestinal Neoplasms', 'Intestine, Small', 'Lymphoma', 'Male', 'Middle Aged', 'Plasma Cells']
| 6,801,847
|
[['C15.378.147.780.490', 'C15.604.515.435', 'C20.683.780.490'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.776.124.486.485.114.619.026.515', 'D12.776.124.486.485.705.500.350', 'D12.776.124.790.651.114.619.026.515', 'D12.776.124.790.651.705.500.350', 'D12.776.377.715.548.114.619.026.515', 'D12.776.377.715.548.705.500.350'], ['C04.588.274.476.411', 'C06.301.371.411', 'C06.405.249.411', 'C06.405.469.491'], ['A03.556.124.684'], ['C04.557.386', 'C15.604.515.569', 'C20.683.515.761'], ['M01.060.116.630'], ['A11.063.438.725', 'A11.118.637.555.567.562.725', 'A15.145.229.637.555.567.562.725', 'A15.382.032.438.725', 'A15.382.490.555.567.562.725']]
|
['Diseases [C]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Named Groups [M]']
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Relationships of foreign protein injections (hyposensitization) in atopic children to serum lipids and lipoproteins.
|
Foreign protein injections (hyposensitization) given for one year to human asymptomatic asthmatics did not increase their serum total lipids or lipoprotein levels, as they apparently did in rabbits fed a lipid-rich, cholesterol-poor diet. Serum total protein and immunoglobulin levels for IgA, IgM and IgG for the asthmatics and controls were no different.
|
['Adolescent', 'Adult', 'Asthma', 'Blood Proteins', 'Child', 'Child, Preschool', 'Cholesterol', 'Desensitization, Immunologic', 'Female', 'Humans', 'Immunoglobulins', 'Lipids', 'Lipoproteins', 'Lipoproteins, HDL', 'Lipoproteins, LDL', 'Lipoproteins, VLDL', 'Male', 'Parents', 'Time Factors', 'Triglycerides']
| 174,460
|
[['M01.060.057'], ['M01.060.116'], ['C08.127.108', 'C08.381.495.108', 'C08.674.095', 'C20.543.480.680.095'], ['D12.776.124'], ['M01.060.406'], ['M01.060.406.448'], ['D04.210.500.247.222.284', 'D04.210.500.247.808.197', 'D10.570.938.208'], ['E02.095.465.425.450.310', 'E05.478.610.310'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.776.124.486.485', 'D12.776.124.790.651', 'D12.776.377.715.548'], ['D10'], ['D10.532', 'D12.776.521'], ['D10.532.432', 'D12.776.521.479'], ['D10.532.515', 'D12.776.521.550'], ['D10.532.599', 'D12.776.521.622'], ['F01.829.263.500.320', 'I01.880.853.150.500.340', 'M01.620'], ['G01.910.857'], ['D10.351.801']]
|
['Named Groups [M]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Psychiatry and Psychology [F]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
|
Botulinum alignment for congenital esotropia.
|
This is the first report of a group of patients with congenital esotropia examined for motor and sensory evidence of binocularity a minimum of 3 years after alignment by botulinum. Evidence for binocularity was clearly present in approximately one half of the patients. Lag time to satisfactory alignment was at least 1 month (average, 5 months) following the initial botulinum injection. The results must be considered preliminary. However, when these results are compared with those of patients with congenital esotropia aligned by incisional surgery by age 2 years and examined with the same testing devices by this same investigator, botulinum alignment appears to be less effective than surgical alignment in establishing evidence for binocularity (P < 0.005).
|
['Botulinum Toxins', 'Child', 'Child, Preschool', 'Depth Perception', 'Esotropia', 'Female', 'Follow-Up Studies', 'Humans', 'Injections', 'Male', 'Oculomotor Muscles', 'Sympathectomy, Chemical', 'Vision Disparity', 'Vision, Binocular', 'Visual Acuity']
| 1,494,828
|
[['D08.811.277.656.300.480.153', 'D08.811.277.656.675.374.153', 'D12.776.097.156', 'D23.946.123.179'], ['M01.060.406'], ['M01.060.406.448'], ['F02.463.593.200', 'F02.463.593.778.255'], ['C10.292.562.887.300', 'C11.590.810.400'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.319.267.530'], ['A02.633.567.700'], ['E04.525.210.105.800.800'], ['F02.463.593.778.255.780', 'F02.463.593.932.877', 'G14.930'], ['F02.463.593.932.885'], ['E01.370.380.850.950', 'F02.463.593.932.901', 'G14.940']]
|
['Chemicals and Drugs [D]', 'Named Groups [M]', 'Psychiatry and Psychology [F]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Anatomy [A]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Determination of toxic heavy metals in sea water by FAAS after preconcentration with a novel chelating resin.
|
A solid phase extraction procedure was developed for preconcentration of toxic heavy metals such as cadmium, cobalt, copper, manganese, lead and zinc in sea water samples. A microcolumn packed with 6-[(4-hydroxyphenyl)diazenyl]naphthalene-2,3-diol-formaldehyde (HPDN-F) resin acts as a sorbent to retain the analyte ions by forming metal chelates. The retained trace level metal was subsequently eluted with 1 mol/L HCl and the acid eluent was analysed by Flame Atomic Absorption Spectrophotometer (FAAS). The HPDN-F chelating resin and its metal chelates were characterized by spectral and thermal analysis. The chelating property of the HPDN-F resin towards divalent metal ions was studied as a function of pH and preconcentration flow rate. The recoveries of cadmium, cobalt, copper, manganese, lead and zinc under the optimum working conditions were above 95%. The relative standard deviations were < 2%. The limits of detection were < 0.1 microg/L. The method presented was applied for the determination of cadmium, cobalt, copper, manganese, lead and zinc in sea water samples.
|
['Chelating Agents', 'Hydrogen-Ion Concentration', 'Magnetic Resonance Spectroscopy', 'Metals, Heavy', 'Seawater', 'Spectrophotometry, Atomic', 'Water Pollutants, Chemical']
| 22,097,064
|
[['D27.505.519.914.500', 'D27.720.832.500'], ['G02.300'], ['E05.196.867.519'], ['D01.268.556', 'D01.552.544'], ['G16.500.275.725.500'], ['E05.196.712.726.551', 'E05.196.867.826.551'], ['D27.888.284.903.655']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Prevalence of clinical rejection after surveillance biopsies in pediatric renal transplants: does early subclinical rejection predispose to subsequent rejection episodes?
|
We analyzed rates of both SCR and CR in children receiving SB at three months post-transplant to determine if SCR predisposed patients to acute CR. Acute rejection was defined according to Banff criteria to include borderline classification or higher. All cases of SCR and CR were treated with anti-rejection protocols. Between October 2004 and July 2008, 89 SB were performed at three months post-transplant. Twenty-six cases of SCR were detected (29%). Sixteen patients experienced 22 episodes of biopsy-proven CR occurring after SB, including seven episodes following SCR and 15 after normal SB. The onset of CR varied from one to 27 months after SB and occurred at similar intervals for cases with SCR and normal SB. The percentage of patients remaining free of CR at 30 months post-transplant was similar in patients with SCR and normal SB. Renal function and graft survival at 30 months also were no different between patients with SCR and those with normal SB. Early-SCR, when treated with rejection protocols, is not a prognostic indicator for subsequent CR episodes.
|
['Adolescent', 'Biopsy', 'Child', 'Child, Preschool', 'Complement C4b', 'Fibrosis', 'Follow-Up Studies', 'Graft Rejection', 'Humans', 'Immunosuppressive Agents', 'Kidney Transplantation', 'Peptide Fragments', 'Prednisone', 'Tacrolimus', 'Transplantation, Homologous', 'Treatment Outcome']
| 19,515,080
|
[['M01.060.057'], ['E01.370.225.500.384.100', 'E01.370.225.998.054', 'E01.370.388.100', 'E04.074', 'E05.200.500.384.100', 'E05.200.998.054', 'E05.242.384.100'], ['M01.060.406'], ['M01.060.406.448'], ['D12.776.124.486.274.350.260'], ['C23.550.355'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['G12.875.545.328'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D27.505.696.477.656'], ['E02.870.500', 'E04.936.450.485', 'E04.950.774.400'], ['D12.644.541'], ['D04.210.500.745.432.719.702'], ['D02.540.505.810'], ['E04.936.864'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
A microRNA biogenesis-like pathway for producing phased small interfering RNA from a long non-coding RNA in rice.
|
Phased small interfering RNAs (phasiRNAs) are a class of non-coding RNAs that perform essential functions in plants. Unlike microRNA biogenesis from a hairpin structure, the production of phasiRNAs usually requires a phase initiator and an RNA-dependent RNA polymerase (RDR) to form double-strand RNAs. By using full-length rice cDNA (KL-cDNA) to identify phasiRNA loci, we found that a putative non-coding sequence with a long hairpin structure generates the phasiRNAs, which we name Long Hairpin-structure containing non-coding RNA (LHR). The biogenesis of LHR-derived phasiRNAs was dependent on rice DCL4, but not on RDR2/6, DCL1, or DCL3. Since all of the LHR-phasiRNAs (-5p from the forward strand and -3p from the reverse strand of the dsRNAs) are mapped to the forward strand of LHR, LHR-phasiRNAs should be derived from its hairpin structure, similar to a microRNA precursor. A degradome-based validation suggested that several thylakoid-related genes were targeted by LHR-phasiRNAs. In addition, the production of LHR-phasiRNAs was completely abolished in the lhr mutant, which also exhibited decreased plant height, leaf size, and grain weight, probably through the regulation of photosynthesis. Based on our results, we propose a microRNA biogenesis-like pathway for producing phased siRNAs that expands our understanding of the current model of phased siRNA biogenesis in plants.
|
['MicroRNAs', 'Oryza', 'RNA, Long Noncoding', 'RNA, Plant', 'RNA, Small Interfering']
| 30,775,774
|
[['D13.150.650.319', 'D13.444.735.150.319', 'D13.444.735.790.552.500'], ['B01.650.940.800.575.912.250.822.616'], ['D13.444.735.790.375'], ['D13.444.735.635'], ['D13.150.650.700', 'D13.444.735.150.700', 'D13.444.735.790.552.875']]
|
['Chemicals and Drugs [D]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Feasibility of a novel radiofrequency signal analysis for in-vivo plaque characterization in humans: comparison of plaque components between patients with and without acute coronary syndrome.
|
BACKGROUND: The iMAP™ is a novel intravascular ultrasound (IVUS)-based technology to classify coronary plaque into 4 components. The aim of this study was to evaluate the feasibility of iMAP™ technology by comparing plaque characteristics in patients with and without acute coronary syndrome (ACS and non-ACS).MATERIALS AND METHODS: A total of 93 culprit lesions from 87 patients were analyzed using the iMAP™. Each plaque was classified into 4 components with a newly introduced parameter, confidence level (CL).RESULTS: iMAP™ analysis of the minimal lumen cross-sectional area (MLA) revealed that ACS lesions had significantly larger lipidic and necrotic areas than non-ACS lesions. Multivariate analysis revealed that the lipidic area at the MLA was an iMAP™ factor independently associated with ACS lesions (odds ratio -1.5, p=0.04). Based on receiver operating characteristic analysis with 4 different CL ranges, the lipidic area at the MLA with 25%-100% CL had the largest area under the curve (0.756), suggesting that 25%-100% is the best CL range for identifying ACS culprit lesions.CONCLUSIONS: The feasibility of the novel iMAP™ IVUS system was shown in discriminating culprit lesions in patients with and without ACS. Analyzing with a CL of 25%-100% may be the best option for discriminating lesions.
|
['Acute Coronary Syndrome', 'Aged', 'Aged, 80 and over', 'Coronary Artery Disease', 'Feasibility Studies', 'Female', 'Humans', 'Male', 'Middle Aged', 'Percutaneous Coronary Intervention', 'Plaque, Atherosclerotic', 'Retrospective Studies', 'Ultrasonography, Interventional']
| 22,578,949
|
[['C14.280.647.124', 'C14.907.585.124'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['C14.280.647.250.260', 'C14.907.137.126.339', 'C14.907.585.250.260'], ['E05.318.372.550', 'E05.337.675', 'N05.715.360.330.550', 'N06.850.520.450.550'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['E04.100.814.529.968', 'E04.502.382.968'], ['C23.300.823'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E01.370.350.850.855', 'E04.502.890']]
|
['Diseases [C]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Computational modeling suggests dimerization of equine infectious anemia virus Rev is required for RNA binding.
|
BACKGROUND: The lentiviral Rev protein mediates nuclear export of intron-containing viral RNAs that encode structural proteins or serve as the viral genome. Following translation, HIV-1 Rev localizes to the nucleus and binds its cognate sequence, termed the Rev-responsive element (RRE), in incompletely spliced viral RNA. Rev subsequently multimerizes along the viral RNA and associates with the cellular Crm1 export machinery to translocate the RNA-protein complex to the cytoplasm. Equine infectious anemia virus (EIAV) Rev is functionally homologous to HIV-1 Rev, but shares very little sequence similarity and differs in domain organization. EIAV Rev also contains a bipartite RNA binding domain comprising two short arginine-rich motifs (designated ARM-1 and ARM-2) spaced 79 residues apart in the amino acid sequence. To gain insight into the topology of the bipartite RNA binding domain, a computational approach was used to model the tertiary structure of EIAV Rev.RESULTS: The tertiary structure of EIAV Rev was modeled using several protein structure prediction and model quality assessment servers. Two types of structures were predicted: an elongated structure with an extended central alpha helix, and a globular structure with a central bundle of helices. Assessment of models on the basis of biophysical properties indicated they were of average quality. In almost all models, ARM-1 and ARM-2 were spatially separated by >15 ?, suggesting that they do not form a single RNA binding interface on the monomer. A highly conserved canonical coiled-coil motif was identified in the central region of EIAV Rev, suggesting that an RNA binding interface could be formed through dimerization of Rev and juxtaposition of ARM-1 and ARM-2. In support of this, purified Rev protein migrated as a dimer in Blue native gels, and mutation of a residue predicted to form a key coiled-coil contact disrupted dimerization and abrogated RNA binding. In contrast, mutation of residues outside the predicted coiled-coil interface had no effect on dimerization or RNA binding.CONCLUSIONS: Our results suggest that EIAV Rev binding to the RRE requires dimerization via a coiled-coil motif to juxtapose two RNA binding motifs, ARM-1 and ARM-2.
|
['Gene Products, rev', 'Infectious Anemia Virus, Equine', 'Models, Molecular', 'Protein Binding', 'Protein Conformation', 'Protein Multimerization', 'RNA, Viral']
| 25,533,001
|
[['D12.776.157.725.076', 'D12.776.664.962.186', 'D12.776.964.925.984.385'], ['B04.820.650.589.520.400'], ['E05.599.595'], ['G02.111.679', 'G03.808'], ['G02.111.570.820.709'], ['G02.111.694'], ['D13.444.735.828']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[Definition, classification and epidemiology of disability].
|
How can we define disability and handicap? What are their different forms? What are the figures for the persons concerned? This paper reviews the key conceptual advances and classification developments as well as new French nationwide surveys on disability. Any attempt at defining what is handicap gives rise to heated debates. This layman word initially originating from the turf language has been prevailing in the medico-social field for the last fifty years. The French 2005 Law on disability has now provided a legal definition inspired by the recommendations of the World Health Organization (WHO). For thirty years, following the pioneering work of Wood, international classifications of disability have been an important activity within the WHO, thus revealing a growing interest for the consequences of health conditions. Following the ICIDH in 1980, WHO has moved towards an interactive model with ICF in 2001. Finally, disability issues open to new directions for epidemiology. In France, the investigations of the INSEE (National Institute on Statistics and Economic Studies), the HID survey "Disabilities-Impairments-Dependence", and tomorrow's "Disability-Health survey, bring issues of disabilities and loss of autonomy at the core of their concerns and allow for a better epidemiologic knowledge of the many faces of the disabled population.
|
['Disability Evaluation', 'Disabled Persons', 'France', 'Humans', 'Prevalence', 'Terminology as Topic']
| 19,894,442
|
[['E01.370.400'], ['M01.150'], ['Z01.542.286'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.308.985.525.750', 'N01.224.935.597.750', 'N06.850.505.400.975.525.750', 'N06.850.520.308.985.525.750'], ['L01.559.598.400']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Named Groups [M]', 'Geographicals [Z]', 'Organisms [B]', 'Health Care [N]', 'Information Science [L]']
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 1
| 1
|
Serum vitamin D level and its relation to thyroid hormone, blood sugar and lipid profiles in Iranian sedentary work staff.
|
INTRODUCTION: the sedentary lifestyle is related to the incidence of various diseases and metabolic disorders. The aim of the current study was to understand the link between serum vitamin D levels, thyroid hormones and lipid profiles among Iranian sedentary staff.MATERIAL AND METHODS: in this cross-sectional study, 300 healthy subjects with normal body mass index (BMI) and age between 18 and 65 years, with sedentary lifestyles, were included. Serum levels of 25-hydroxyvitamin D, thyroid stimulating hormone (TSH), fasting blood sugar, plasma total cholesterol, and high-density lipoprotein (HDL) and triglycerides (TG) were measured with qualified laboratory methods. Low-density lipoprotein (LDL) concentration was calculated based on the Friedewald equation. A self-made questionnaire with different questions was used to assess physical activity.RESULTS: the means of BMI and age were 25.63 ± 10.25 and 36.69 ± 7.14 years, respectively. The prevalence of vitamin D deficiency was 65.7%. Results showed significant differences for TG, HDL, and thyroxine (T4) between subgroup categories. Serum levels of 25-hydroxyvitamin D had a negative significant correlation with triiodothyronine (T3) and T4, and a positive correlation with HDL. Linear regression analysis showed a significant association of 25-hydroxyvitamin D concentrations with HDL and T4 after adjustments based on the sex.CONCLUSION: finally, the results of this study show that with the improvement in vitamin D status, the decrease in the levels of TG, T3 and T4, with an increase in HDL can be expected. So, verification and detection of true causality through the interventional studies will be valuable, scientifically.
|
['Adolescent', 'Adult', 'Aged', 'Blood Glucose', 'Body Mass Index', 'Cross-Sectional Studies', 'Exercise', 'Female', 'Humans', 'Iran', 'Lipids', 'Male', 'Middle Aged', 'Nutritional Status', 'Sedentary Behavior', 'Thyroid Hormones', 'Vitamin D', 'Vitamin D Deficiency', 'Young Adult']
| 30,307,294
|
[['M01.060.057'], ['M01.060.116'], ['M01.060.116.100'], ['D09.947.875.359.448.500'], ['E01.370.600.115.100.125', 'E05.041.124.125', 'G07.100.100.125', 'N06.850.505.200.100.175'], ['E05.318.372.500.875', 'N05.715.360.330.500.875', 'N06.850.520.450.500.875'], ['G11.427.410.698.277', 'I03.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['Z01.252.245.500.350'], ['D10'], ['M01.060.116.630'], ['G07.203.650.650', 'N01.224.425.525'], ['F01.145.749', 'F01.829.458.705'], ['D06.472.931'], ['D04.210.500.812.768'], ['C18.654.521.500.133.770'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Organisms [B]', 'Geographicals [Z]', 'Psychiatry and Psychology [F]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 1
|
Synthesis and biological studies of glycosyl dopamine derivatives as potential antiparkinsonian agents.
|
A new approach to deliver dopamine into the central nervous system, based on the use of D-glucose as transportable agent, has been studied. Glycosyl dopamine derivatives bearing the sugar moiety linked to either the amino group or the catechol ring of dopamine through amide, ester or glycosidic bonds were synthesised as potential antiparkinsonian agents. Studies on the binding to dopamine D2 receptor, in vitro stability, and locomotive effect in mice of the synthetic glycoconjugates are reported.
|
['Animals', 'Binding, Competitive', 'Biological Availability', 'Biological Transport', 'Blood-Brain Barrier', 'Dopamine', 'Dopamine Agents', 'Drug Stability', 'Glucose', 'Glycosides', 'Mice', 'Motor Activity', 'Parkinson Disease', 'Prodrugs', 'Rats', 'Receptors, Dopamine D2']
| 10,990,020
|
[['B01.050'], ['E05.196.080', 'G02.111.084', 'G02.111.570.120.309'], ['G03.787.151', 'G07.690.725.129'], ['G03.143'], ['A07.035', 'A08.186.211.035'], ['D02.092.211.215.406', 'D02.092.311.342', 'D02.455.426.559.389.657.166.175.342'], ['D27.505.519.625.150', 'D27.505.696.577.150'], ['E05.916.330'], ['D09.947.875.359.448'], ['D09.408'], ['B01.050.150.900.649.313.992.635.505.500'], ['F01.145.632', 'G11.427.410.698'], ['C10.228.140.079.862.500', 'C10.228.662.600.400', 'C10.574.928.750'], ['D26.675'], ['B01.050.150.900.649.313.992.635.505.700'], ['D12.776.543.750.670.300.400.500', 'D12.776.543.750.695.150.400.500', 'D12.776.543.750.720.330.400.500']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Psychiatry and Psychology [F]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Anti diabetic effect of cherries in alloxan induced diabetic rats.
|
Diabetes mellitus (DM) is a metabolic disorder in the endocrine system resulting from a defect in insulin secretion, insulin action or both of them. Adverse side effects of chemical drugs for treatment of diabetes persuaded the using of medical plants. Cherry as a traditionally used plant for treatment of diabetes, is packed with powerful plant pigments called anthocyanins. They give cherries their dark red color and are one of the richest antioxidant sources which lower the blood sugar and bear other beneficial health effects. The purpose of this study is to evaluate the effect of ethanolic extract of cherry fruit on alloxan induced diabetic rats. In this study 36 Male Wistar rats, body weight of 150-200gr were divided into 6 groups. Diabetes was induced by intra peritoneal injection of 120 mg/kg Alloxan. The duration of the cherries treatment was 30 days in which single dose of extracts (200mg/kg) were oral administered to diabetic rats. Blood glucose levels were estimated with glucometer before treatment, 2h and 1- 4 weeks after administration of extracts. Treatment with extracts of the cherries resulted in a significant reduction in blood glucose and urinary microalbumin and an increase in the creatinine secretion level in urea. Extract of this plant is useful in controlling the blood glucose level. Cherries appear to aid in diabetes control and diminution of the complications of the disease. Some relevant patents are also outlined in this article.
|
['Administration, Oral', 'Albuminuria', 'Alloxan', 'Animals', 'Biomarkers', 'Blood Glucose', 'Creatinine', 'Diabetes Mellitus, Experimental', 'Diabetic Nephropathies', 'Fruit', 'Hypoglycemic Agents', 'Male', 'Plant Extracts', 'Prunus', 'Rats', 'Rats, Wistar', 'Time Factors']
| 22,280,223
|
[['E02.319.267.100'], ['C12.777.934.734.269', 'C13.351.968.934.734.269', 'C23.888.942.750.269'], ['D03.383.742.698.122'], ['B01.050'], ['D23.101'], ['D09.947.875.359.448.500'], ['D03.383.129.308.207'], ['C18.452.394.750.074', 'C19.246.240', 'E05.598.500.374'], ['C12.777.419.192', 'C13.351.968.419.192', 'C19.246.099.875'], ['A18.024.500', 'G07.203.300.562', 'J02.500.562'], ['D27.505.696.422'], ['D20.215.784.500', 'D26.667'], ['B01.650.940.800.575.912.250.859.937.500.625'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.900'], ['G01.910.857']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Technology, Industry, and Agriculture [J]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Human T-cell leukemia virus type 1 tax dysregulates beta-catenin signaling.
|
Dysregulation of beta-catenin signaling has been implicated in the malignant transformation of cells. However, the role of beta-catenin in the human T-cell leukemia virus type 1 (HTLV-1)-induced transformation of T cells is unknown. Here we found that beta-catenin protein was overexpressed in the nucleus and that beta-catenin-dependent transcription was significantly enhanced in Tax-positive HTLV-1-infected T-cell lines compared to that in Tax-negative HTLV-1-infected T-cell lines. Transfection with beta-catenin-specific small interfering RNA inhibited the growth of the Tax-positive HTLV-1-infected T-cell line HUT-102. Transient transfection of Tax appeared to enhance beta-catenin-dependent transcription by stabilizing the beta-catenin protein via activation of the cyclic AMP (cAMP) response element-binding protein. HTLV-1-infected T-cell lines overexpressing beta-catenin also showed increased Akt activity via Tax activation of the cAMP response element-binding protein, resulting in the phosphorylation and inactivation of glycogen synthase kinase 3beta, which phosphorylates beta-catenin for ubiquitination. The phosphatidylinositol 3-kinase inhibitor LY294002 reduced beta-catenin expression in Tax-positive T-cell lines, and inactivation of glycogen synthase kinase 3beta by lithium chloride restored beta-catenin expression in Tax-negative T-cell lines. Finally, we showed that dominant-negative Akt inhibited Tax-induced beta-catenin-dependent transcription. These results indicate that Tax activates beta-catenin through the Akt signaling pathway. Our findings suggest that activation of beta-catenin by Tax may be important in the transformation of T cells by HTLV-1 infection.
|
['Base Sequence', 'Cell Line', 'Cell Proliferation', 'Cell Transformation, Neoplastic', 'Cyclic AMP Response Element-Binding Protein', 'DNA', 'DNA, Viral', 'Gene Products, tax', 'Genes, pX', 'HeLa Cells', 'Human T-lymphotropic virus 1', 'Humans', 'Models, Biological', 'Phosphatidylinositol 3-Kinases', 'Proteasome Inhibitors', 'Proto-Oncogene Proteins c-akt', 'Signal Transduction', 'T-Lymphocytes', 'TCF Transcription Factors', 'Transcription, Genetic', 'Transfection', 'beta Catenin']
| 16,920,823
|
[['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['A11.251.210'], ['G04.161.750', 'G07.345.249.410.750'], ['C04.697.098.500', 'C23.550.727.098.500'], ['D12.776.260.108.184', 'D12.776.930.127.184'], ['D13.444.308'], ['D13.444.308.568'], ['D12.776.624.664.520.750.480', 'D12.776.964.700.750.480', 'D12.776.964.775.750.480', 'D12.776.964.925.984.410'], ['G05.360.340.024.340.364.875.735', 'G05.360.340.024.340.425.416', 'G05.360.340.358.024.875.735', 'G05.360.340.358.840.500.735'], ['A11.251.210.190.400', 'A11.251.860.180.400', 'A11.436.340'], ['B04.613.807.200.725.400', 'B04.820.650.200.725.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.599.395'], ['D08.811.913.696.620.500'], ['D27.505.519.389.745.705'], ['D08.811.913.696.620.682.700.755', 'D12.776.476.565', 'D12.776.624.664.700.168'], ['G02.111.820', 'G04.835'], ['A11.118.637.555.567.569', 'A15.145.229.637.555.567.569', 'A15.382.490.555.567.569'], ['D12.776.260.730', 'D12.776.660.235.400.800', 'D12.776.664.235.400.800', 'D12.776.930.875'], ['G02.111.873', 'G05.297.700'], ['E05.393.350.810', 'G05.728.860'], ['D12.776.091.249', 'D12.776.220.145.500', 'D12.776.930.130']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Anatomy [A]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Enhanced Fano resonance of organic material films deposited on arrays of asymmetric split-ring resonators (A-SRRs).
|
Depositing very thin organic films on the surface of arrays of asymmetric split-ring resonators (A-SRRs) produces a shift in their resonance spectra that can be utilized for sensitive analyte detection. Here we show that when poly-methyl-methacrylate (PMMA) is used as an organic probe (analyte) on top of the A-SRR array, the phase and amplitude of a characteristic molecular Fano resonance associated with a carbonyl bond changes according to the spectral positions of the trapped mode resonance of the A-SRRs and their plasmonic reflection peaks. Furthermore, we localize blocks of PMMA at different locations on the A-SRR array to determine the effectiveness of detection of very small amounts of non-uniformly distributed analyte.
|
['Equipment Design', 'Equipment Failure Analysis', 'Metals', 'Polymethyl Methacrylate', 'Refractometry', 'Surface Plasmon Resonance']
| 23,609,645
|
[['E05.320'], ['E05.325.192'], ['D01.552'], ['D02.241.081.069.800.550.500', 'D05.750.716.822.111.650.605.500', 'D25.720.716.822.111.650.605.500', 'J01.637.051.720.716.822.111.650.605.500'], ['E05.196.808', 'H01.671.617.755'], ['E05.196.890', 'E05.601.043.700']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Technology, Industry, and Agriculture [J]', 'Disciplines and Occupations [H]']
| 0
| 0
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
|
Claudin-3-deficient C57BL/6J mice display intact brain barriers.
|
The tight junction protein claudin-3 has been identified as a transcriptional target of the Wnt/â-catenin signaling pathway regulating blood-brain barrier (BBB) maturation. In neurological disorders loss of claudin-3 immunostaining is observed at the compromised BBB and blood-cerebrospinal fluid barrier (BCSFB). Although these observations support a central role of claudin-3 in regulating brain barriers' tight junction integrity, expression of claudin-3 at the brain barriers has remained a matter of debate. This prompted us to establish claudin-3-/- C57BL/6J mice to study the role of claudin-3 in brain barrier integrity in health and neuroinflammation. Bulk and single cell RNA sequencing and direct comparative qRT-PCR analysis of brain microvascular samples from WT and claudin-3-/- mice show beyond doubt that brain endothelial cells do not express claudin-3 mRNA. Detection of claudin-3 protein at the BBB in vivo and in vitro is rather due to junctional reactivity of anti-claudin-3 antibodies to an unknown antigen still detected in claudin-3-/- brain endothelium. We confirm expression and junctional localization of claudin-3 at the BCSFB of the choroid plexus. Our study clarifies that claudin-3 is not expressed at the BBB and shows that absence of claudin-3 does not impair brain barrier function during health and neuroinflammation in C57BL/6J mice.
|
['Animals', 'Biological Transport', 'Blood-Brain Barrier', 'Brain', 'Choroid Plexus', 'Claudin-3', 'Endothelial Cells', 'Female', 'Male', 'Mice', 'Mice, Inbred C57BL', 'Tight Junction Proteins', 'Tight Junctions', 'Wnt Signaling Pathway']
| 30,659,216
|
[['B01.050'], ['G03.143'], ['A07.035', 'A08.186.211.035'], ['A08.186.211'], ['A08.186.211.140.298'], ['D12.776.543.940.200.300'], ['A11.436.275'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.420', 'B01.050.150.900.649.313.992.635.505.500.400.420'], ['D12.776.543.940'], ['A11.284.149.165.420.820'], ['G02.111.820.925', 'G04.835.925']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Chemicals and Drugs [D]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Hydroxychloroquine in steroid dependent asthma.
|
A recent case report suggested that hydroxychloroquine had a steroid sparing effect in a patient with severe chronic asthma. We have studied the effect of hydroxychloroquine in a group of nine steroid dependent adult asthmatic patients using a randomised double blind crossover comparison of hydroxychloroquine and placebo. Each patient received hydroxychloroquine (400 mg/day) or placebo for 2 month periods. The effect of hydroxychloroquine or placebo on asthma control was assessed by change in steroid dosage, visual analogue symptom scores, response to beta 2 agonist and peak expiratory flow rate (PFR) measurement. The dose of prednisolone required during hydroxychloroquine treatment did not differ from that during placebo treatment or in pre-trial period. There was no significant change in symptom scores of PFR measurement. In this study an 8 week treatment with hydroxychloroquine was of no benefit to patients with chronic steroid dependent asthma.
|
['Adult', 'Asthma', 'Beclomethasone', 'Double-Blind Method', 'Female', 'Humans', 'Hydroxychloroquine', 'Male', 'Middle Aged', 'Steroids']
| 2,980,288
|
[['M01.060.116'], ['C08.127.108', 'C08.381.495.108', 'C08.674.095', 'C20.543.480.680.095'], ['D04.210.500.745.432.769.125', 'D04.210.500.883.154'], ['E05.318.370.300', 'E05.581.500.300', 'N05.715.360.325.320', 'N06.850.520.445.300'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D03.633.100.810.050.180.350'], ['M01.060.116.630'], ['D04.210.500']]
|
['Named Groups [M]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Distribution of [125I]nerve growth factor in the rat brain following a single intraventricular injection: correlation with the topographical distribution of trkA messenger RNA-expressing cells.
|
The present study determined the topographical distribution of [125I] nerve growth factor in rat brain at various time points following an intraventricular injection. In addition, we quantified the tissue content of nerve growth factor in various brain tissues following the injection. Autoradiographic analysis of the distribution of [125] nerve growth factor indicated that the neurotrophin is rapidly distributed within the entire ventricular system. However, penetration of nerve growth factor into the brain parenchyma was very limited. At early time points following an injection of nerve growth factor, there was an accumulation of label in the immediate vicinity of the lateral ventricle and third ventricle with predominant labeling around the septum, hypothalamus and cerebellum. By 24 h following nerve growth factor administration, there was discreet labeling of the lateral septum, medial septum, diagonal band, hypothalamus, olfactory tubercle and nucleus of the olfactory tract, and some label was present in the hippocampus and subiculum. Quantitative ELISA of nerve growth factor in brain tissues 1 h following the injection indicated a 446% and 133% increase over basal levels of nerve growth factor in the basal forebrain and hippocampus, respectively. At 24 h nerve growth factor levels measured in brain were not significantly different from endogenous basal levels as determined by ELISA, whereas there were high quantities of 125I present in the thyroid gland, suggesting that the administered [125I] nerve growth factor was rapidly degraded following the intraventricular injection. We observed a similar labeling pattern of the medial septum/diagonal band cholinergic cell body group 24 h following either an intraventricular or intrahippocampal injection of [125I] nerve growth factor. There was a good correlation between the [125I] nerve growth factor labeling pattern and the presence of trkA messenger RNA. This suggested that, at least in the septohippocampal pathway, nerve growth factor accumulated in a region which contained trkA nerve growth factor receptors. Thus, this study shows that after a single unilateral intraventricular injection of nerve growth factor into rat brain there is effective uptake by diagonal band/septal cells on both sides of the brain, and by cells whose positions correlate with the locations of cholinergic and trk A messenger RNA-expressing cells. Significant uptake was also observed in the hypothalamus and cerebellum. The very limited penetration and rapid degradation of intraventricularly administered nerve growth factor suggests that tissue penetration may be a limiting factor when attempting to influence brain neurons by exogenous neurotropic factors.
|
['Animals', 'Autoradiography', 'Brain', 'Cerebral Ventricles', 'Enzyme-Linked Immunosorbent Assay', 'Female', 'Gene Expression', 'In Situ Hybridization', 'Injections, Intraventricular', 'Iodine Radioisotopes', 'Kinetics', 'Nerve Growth Factors', 'Organ Specificity', 'Proto-Oncogene Proteins', 'RNA, Messenger', 'Rats', 'Rats, Wistar', 'Receptor Protein-Tyrosine Kinases', 'Receptor, trkA', 'Receptors, Nerve Growth Factor', 'Tissue Distribution']
| 8,336,831
|
[['B01.050'], ['E01.370.225.750.132', 'E05.200.750.132', 'E05.799.256'], ['A08.186.211'], ['A08.186.211.140'], ['E05.478.566.350.170', 'E05.478.566.380.360', 'E05.478.583.400.170', 'E05.601.470.350.170', 'E05.601.470.380.360'], ['G05.297'], ['E01.370.225.500.620.670.325', 'E01.370.225.750.600.670.325', 'E05.200.500.620.670.325', 'E05.200.750.600.670.325', 'E05.393.661.475'], ['E02.319.267.530.550'], ['D01.268.380.400.500.496', 'D01.496.448.496', 'D01.496.749.474'], ['G01.374.661', 'G02.111.490'], ['D12.644.276.860', 'D12.776.467.860', 'D12.776.631.600', 'D23.529.850'], ['G07.650'], ['D12.776.624.664.700'], ['D13.444.735.544'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.900'], ['D08.811.913.696.620.682.725.400', 'D12.776.543.750.630'], ['D08.811.913.696.620.682.725.400.660', 'D12.776.543.750.630.496', 'D12.776.543.750.750.400.550.550'], ['D12.776.543.750.750.400.550'], ['G03.787.917', 'G07.690.725.949']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Antibody Fragments for On-Site Testing of Cannabinoids Generated via in Vitro Affinity Maturation.
|
Law enforcement against illicit use of cannabis and related substances requires rapid, feasible, and reliable tools for on-site testing of cannabinoids. Notably, methods based on cannabinoid-specific antibodies enable efficient screening of multiple specimens. Antibody engineering may accelerate development of modern and robust testing systems. Here, we used in vitro affinity maturation to generate a single-chain Fv fragment (scFv) that recognizes with high affinity the psychoactive cannabinoid, Ä9-tetrahydrocannabinol (THC). A mouse monoclonal antibody against THC, Ab-THC#33, with Ka 6.2?107 M-1 (as Fab fragment) was established by the hybridoma technique. Then, a "wild-type" scFv (wt-scFv) with Ka, 1.1?107 M-1 was prepared by bacterial expression of a fusion gene combining the VH and VL genes for Ab-THC#33. Subsequently, random point mutations in VH and VL were generated separately, and the resulting products were assembled into mutant scFv genes, which were then phage-displayed. Repeated panning identified a mutant scFv (scFv#m1-36) with 10-fold enhanced affinity (Ka 1.1?108 M-1) for THC, in which only a single conservative substitution (Ser50Thr) was present at the N-terminus of the VH-complementarity-determining region 2 (CDR2) sequence. In competitive enzyme-linked immunosorbent assay (ELISA), the mutant scFv generated dose-response curves with midpoint 0.27 ng/assay THC, which was 3-fold lower than that of wt-scFv. Even higher reactivity with a major THC metabolite, 11-nor-9-carboxy-Ä9-tetrahydrocannabinol, indicated that the mutant scFv will be useful for testing not only THC in confiscated materials, but also the metabolite in urine. Indeed, the antibody fragment is potentially suitable for use in advanced on-site testing platforms for cannabinoids.
|
['Amino Acid Sequence', 'Animals', 'Antibodies, Monoclonal', 'Antibody Affinity', 'Cannabinoids', 'Dose-Response Relationship, Drug', 'Female', 'Immunoglobulin Fragments', 'Mice', 'Mice, Inbred BALB C', 'Molecular Docking Simulation', 'Protein Structure, Secondary', 'Substance Abuse Detection']
| 28,154,257
|
[['G02.111.570.060', 'L01.453.245.667.060'], ['B01.050'], ['D12.776.124.486.485.114.224', 'D12.776.124.790.651.114.224', 'D12.776.377.715.548.114.224'], ['G12.040', 'G12.122.125'], ['D02.455.849.090'], ['G07.690.773.875', 'G07.690.936.500'], ['D12.644.541.500', 'D12.776.124.486.485.680', 'D12.776.124.790.651.680', 'D12.776.377.715.548.680'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.338', 'B01.050.150.900.649.313.992.635.505.500.400.338'], ['E05.599.595.249', 'L01.224.160.249'], ['G02.111.570.820.709.600'], ['E05.885', 'N06.850.780.500.765']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 1
| 0
|
Academy of Nutrition and Dietetics: Revised 2012 Standards of Practice in Nutrition Care and Standards of Professional Performance for Dietetic Technicians, Registered.
|
DTRs face complex situations every day. Competently addressing the unique needs of each situation and applying standards appropriately is essential to providing safe, timely, person-centered quality care and service. All DTRs are advised to conduct their practice based on the most recent edition of the Academy's Code of Ethics and the Scope of Practice in Nutrition and Dietetics, the Scope of Practice for the DTR, the 2012 Standards of Practice in Nutrition Care and Standards of Professional Performance for DTRs. These resources provide minimum standards and tools for demonstrating competence and safe practice, and are used collectively to gauge and guide a DTR's performance in nutrition and dietetics practice. The SOP and SOPP for the DTR are self-evaluation tools that promote quality assurance and performance improvement. Self-assessment provides opportunities to identify areas for enhancement, new learning and skill development, and to encourage progression of career growth. All DTRs are advised to have in their personal libraries the most recent copy of the Academy's Scope of Practice in Nutrition and Dietetics and its components: The 2012 Academy Standards of Practice in Nutrition Care and Standards of Professional Performance for DTRs; the Code of Ethics; and the Scope of Practice for the DTR. To ensure that credentialed dietetics practitioners always have access to the most current materials, each resource is maintained on the Academy's website. The documents will continue to be reviewed and updated as new trends in the profession of nutrition and dietetics and external influences emerge.
|
['Clinical Competence', 'Dietetics', 'Humans', 'Quality of Health Care', 'United States']
| 23,454,022
|
[['I02.399.630.210', 'N04.761.210', 'N05.715.175'], ['H02.533.290'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['N04.761', 'N05.715'], ['Z01.107.567.875']]
|
['Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]', 'Disciplines and Occupations [H]', 'Organisms [B]', 'Geographicals [Z]']
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 1
| 1
|
Epithelial phosphatidylinositol-3-kinase signaling is required for â-catenin activation and host defense against Citrobacter rodentium infection.
|
Citrobacter rodentium infection of mice induces cell-mediated immune responses associated with crypt hyperplasia and epithelial â-catenin signaling. Recent data suggest that phosphatidylinositol-3-kinase (PI3K)/Akt signaling cooperates with Wnt to activate â-catenin in intestinal stem and progenitor cells through phosphorylation at Ser552 (P-â-catenin(552)). Our aim was to determine whether epithelial PI3K/Akt activation is required for â-catenin signaling and host defense against C. rodentium. C57BL/6 mice were infected with C. rodentium and treated with dimethyl sulfoxide (DMSO) (vehicle control) or with the PI3K inhibitor LY294002 or wortmannin. The effects of infection on PI3K activation and â-catenin signaling were analyzed by immunohistochemistry. The effects of PI3K inhibition on host defense were analyzed by the quantification of splenic and colon bacterial clearance, and adaptive immune responses were measured by real-time PCR (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). Increased numbers of P-â-catenin(552)-stained epithelial cells were found throughout expanded crypts in C. rodentium colitis. We show that the inhibition of PI3K signaling attenuates epithelial Akt activation, the Ser552 phosphorylation and activation of â-catenin, and epithelial cell proliferative responses during C. rodentium infection. PI3K inhibition impairs bacterial clearance despite having no impact on mucosal cytokine (gamma interferon [IFN-ã], tumor necrosis factor [TNF], interleukin-17 [IL-17], and IL-1â) or chemokine (CXCL1, CXCL5, CXCL9, and CXCL10) induction. The results suggest that the host defense against C. rodentium requires epithelial PI3K activation to induce Akt-mediated â-catenin signaling and the clearance of C. rodentium independent of adaptive immune responses.
|
['Animals', 'Citrobacter rodentium', 'Colitis', 'Enterobacteriaceae Infections', 'Enzyme Activation', 'Enzyme Inhibitors', 'Enzyme-Linked Immunosorbent Assay', 'Epithelial Cells', 'Fluorescent Antibody Technique', 'Immunohistochemistry', 'Mice', 'Mice, Inbred C57BL', 'Phosphatidylinositol 3-Kinases', 'Proto-Oncogene Proteins c-akt', 'Reverse Transcriptase Polymerase Chain Reaction', 'Signal Transduction', 'beta Catenin']
| 21,343,355
|
[['B01.050'], ['B03.440.450.425.200.737', 'B03.660.250.150.100.737'], ['C06.405.205.265', 'C06.405.469.158.188'], ['C01.150.252.400.310'], ['G02.111.263', 'G03.328'], ['D27.505.519.389'], ['E05.478.566.350.170', 'E05.478.566.380.360', 'E05.478.583.400.170', 'E05.601.470.350.170', 'E05.601.470.380.360'], ['A11.436'], ['E01.370.225.500.607.512.240', 'E01.370.225.750.551.512.240', 'E05.200.500.607.512.240', 'E05.200.750.551.512.240', 'E05.478.583.375'], ['E01.370.225.500.607.512', 'E01.370.225.750.551.512', 'E05.200.500.607.512', 'E05.200.750.551.512', 'E05.478.583', 'H01.158.100.656.234.512', 'H01.158.201.344.512', 'H01.158.201.486.512', 'H01.181.122.573.512', 'H01.181.122.605.512'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.420', 'B01.050.150.900.649.313.992.635.505.500.400.420'], ['D08.811.913.696.620.500'], ['D08.811.913.696.620.682.700.755', 'D12.776.476.565', 'D12.776.624.664.700.168'], ['E05.393.620.500.725'], ['G02.111.820', 'G04.835'], ['D12.776.091.249', 'D12.776.220.145.500', 'D12.776.930.130']]
|
['Organisms [B]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Disciplines and Occupations [H]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
Fiber-type effects of castration on the cholinergic receptor population in skeletal muscle.
|
The effects of castration on the extensor digitorum longus, soleus and levator ani muscles of the adult male rat were examined. After castration, no change was observed in the number of sites or the binding affinity of [125I]-monoiodo-alpha-bungarotoxin to the nicotinic cholinergic receptor population of the slow fiber muscle (soleus). The two fast fiber muscles (extensor and levator) showed no change in binding affinity but a significant change in the number of sites. In these two fast fiber muscles, the number of sites begins to increase within the first few days after castration, peaks at 14 days and begins to decline. The results presented in this report demonstrate three major concepts: 1) influences on skeletal muscle mediated by one receptor population can change the characteristics of another receptor population; 2) at least some androgen effects on skeletal muscle are fiber type specific; and 3) the development of extrajunctional receptors is not solely the function of the nerve-muscle relationship.
|
['Animals', 'Binding, Competitive', 'Bungarotoxins', 'Castration', 'Male', 'Muscles', 'Organ Specificity', 'Parasympathomimetics', 'Rats', 'Receptors, Cholinergic', 'Time Factors']
| 7,359,355
|
[['B01.050'], ['E05.196.080', 'G02.111.084', 'G02.111.570.120.309'], ['D20.888.850.325.139', 'D23.946.833.850.325.139'], ['E04.270.282', 'E04.950.165'], ['A02.633', 'A10.690'], ['G07.650'], ['D27.505.696.663.050.675'], ['B01.050.150.900.649.313.992.635.505.700'], ['D12.776.543.750.720.360'], ['G01.910.857']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
The effects of critical thinking instruction on training complex decision making.
|
OBJECTIVE: Two field studies assessed the effects of critical thinking instruction on training and transfer of a complex decision-making skill.BACKGROUND: Critical thinking instruction is based on studies of how experienced decision makers approach complex problems.METHOD: Participants conducted scenario-based exercises in both simplified (Study I) and high-fidelity (Study 2) training environments. In both studies, half of the participants received instruction in critical thinking. The other half conducted the same exercises but without critical thinking instruction. After the training, test scenarios were administered to both groups.RESULTS: The first study showed that critical thinking instruction enhanced decision outcomes during both training and the test. In the second study, critical thinking instruction benefited both decision outcomes and processes, specifically on the transfer to untrained problems.CONCLUSION: The results suggest that critical thinking instruction improves decision strategy and enhances understanding of the general principles of the domain.APPLICATION: The results of this study warrant the implementation of critical thinking instruction in training programs for professional decision makers that have to operate in complex and highly interactive, dynamic environments.
|
['Decision Making', 'Humans', 'Military Personnel', 'Netherlands', 'Policy Making', 'Task Performance and Analysis', 'Thinking']
| 21,141,245
|
[['F02.463.785.373'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.526.625'], ['Z01.542.651'], ['N03.706.742'], ['F02.784.412.846', 'F02.784.692.746', 'F02.808.600'], ['F02.463.785']]
|
['Psychiatry and Psychology [F]', 'Organisms [B]', 'Named Groups [M]', 'Geographicals [Z]', 'Health Care [N]']
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Intrastriatal grafts from multiple donors do not result in a proportional increase in survival of dopamine neurons in nonhuman primates.
|
We examined the potential for "double grafts," i.e., grafts from two donors in each recipient, to enhance the total number of ventral mesencephalic dopamine neurons that survive grafting in adult African green monkeys. Because dopamine cell survival in grafts represents a small percentage of the total number of neurons grafted, several human clinical trials recently have employed grafts of tissue from multiple donors (e.g., from two to eight embryos per host recipient) in attempts to increase the total number of dopamine neurons that survive in grafts. Presumably, this is intended to elevate dopamine levels by providing more dopamine neurons to the damaged brain to alleviate the symptoms of parkinsonism. While well-developed grafts with several thousand dopamine neurons were found in most recipient animals, we observed a reduced total number of tyrosine hydroxylase positive neurons in the grafts in spite of the presence of some double grafts that were larger than normal. The overall growth of the grafts was impressive; some grafts were so large that they spanned the full dorsoventral extent of the caudate nucleus, probably reflecting the fact that twice as much tissue was implanted in each drop site in comparison to our standard protocol. However, some animals revealed atypical patterns of neurite outgrowth that appeared limited to the grafted tissue, and at least one monkey revealed "amorphous" grafts generally lacking in cellular structure, which suggests a possible rejection phenomenon. These findings raise questions about the use of multiple donors and suggest that the likelihood of rejection and/or cell death may be enhanced, which is of potential importance in the design of grafting strategies for clinical applications.
|
['Animals', 'Brain Tissue Transplantation', 'Cell Count', 'Cell Survival', 'Chlorocebus aethiops', 'Dopamine', 'Fetal Tissue Transplantation', 'Graft Rejection', 'Graft Survival', 'Humans', 'Neurons', 'Tissue Donors']
| 9,588,591
|
[['B01.050'], ['E02.095.147.725.090', 'E04.525.090', 'E04.936.580.090'], ['E01.370.225.500.195', 'E05.200.500.195', 'E05.242.195', 'G04.140'], ['G04.346'], ['B01.050.150.900.649.313.988.400.112.199.120.126.110'], ['D02.092.211.215.406', 'D02.092.311.342', 'D02.455.426.559.389.657.166.175.342'], ['E02.095.147.725.300', 'E04.936.580.300'], ['G12.875.545.328'], ['G12.875.545.340'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A08.675', 'A11.671'], ['M01.898']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Named Groups [M]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Patient-centred education: what do students think?
|
CONTEXT: Medical educators endeavour to foster patient-centred learning. Although studies of patient-educators report general increases in patient-centredness, no formal review of students' reflections on the role of patients in their education has yet been undertaken. Our research questions were: (i) What themes might be identified through a qualitative analysis of students' reflective writing on patient-centred education? (ii) What are common students' perceptions regarding patients as educators?METHODS: For two academic years, Year 2 pre-clinical students (189 and 167 students, respectively, in each academic year) submitted a 250-word writing assignment in response to one of four questions meant to promote reflection on the role of patients in their education. Using a grounded theory approach, we performed a qualitative analysis of these written reflections for emerging themes. A synthesis of these themes was prepared and was presented for validation and discussion by two focus groups of six and three students, respectively. We analysed the transcripts of the focus group discussions and compared them with results from the analysis of written reflections and used them to further inform and refine our initial thematic framework.RESULTS: A total of 356 reflective writing assignments were analysed. The major themes were: (i) students seeing the condition within the context of patients' lives; (ii) patients supporting students' learning; (iii) students recognising patients' needs; (iv) students seeing the patient as a capable part of the team, and (v) students recognising the complexity of practising medicine. The two focus group discussions confirmed these main themes, but placed greater emphasis on the first and second themes. These themes mapped closely to the conceptualisation of patient-centred care defined by the International Alliance of Patients' Organizations.CONCLUSIONS: Students' reflections on their experiences of patient-educators cover an important and broad range of key concepts in patient-centred care that are well aligned with patient-generated conceptualisations of patient-centred care.
|
['Attitude of Health Personnel', 'Curriculum', 'Education, Medical', 'Female', 'Focus Groups', 'Humans', 'Male', 'Patient-Centered Care', 'Physician-Patient Relations', 'Qualitative Research', 'Role', 'Students, Medical', 'Teaching', 'Writing']
| 24,528,399
|
[['F01.100.050', 'N05.300.100'], ['I02.158'], ['I02.358.399'], ['E05.318.308.112', 'N05.715.360.300.269', 'N06.850.520.308.112'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['N04.590.233.727.407'], ['F01.829.401.650.675', 'N05.300.660.625'], ['H01.770.644.241.850'], ['F01.829.316.616'], ['M01.848.769.602'], ['I02.903'], ['L01.559.423.906']]
|
['Psychiatry and Psychology [F]', 'Health Care [N]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Disciplines and Occupations [H]', 'Named Groups [M]', 'Information Science [L]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 1
| 0
|
The influence of residents training level on their evaluation of clinical teaching faculty.
|
BACKGROUND: Evaluations by learners are the most common sources of information on teaching. There is some debate about the role of these assessments, but the overall evaluation of faculty by learners was found to be valid and reliable.PURPOSE: The purpose of this study was to examine the relationship between the level of training of family medicine residents and their evaluation of emergency medicine clinical teachers over time.METHODS: A prospective cohort analysis of 6 years of faculty evaluation of 115 teachers was conducted.RESULTS: The 562 residents returned 3,046 valid individual evaluations. There was no significant association between the level of residents' training and the ratings for clinical instruction (p > .05). Resident evaluations did not vary by time of year (p > .05); however, they did significantly differ by year of evaluation, showing that ratings increased over the 6 years of the study (p < .0001).CONCLUSIONS: Neither the residents' level of training nor the timing during the academic year were significant independent predictors of perceived superior teaching performance, although ratings increased over the 6 years of the study.
|
['Alberta', 'Cohort Studies', 'Data Collection', 'Emergency Medicine', 'Faculty', 'Internship and Residency']
| 15,691,813
|
[['Z01.107.567.176.064'], ['E05.318.372.500.750', 'N05.715.360.330.500.750', 'N06.850.520.450.500.750'], ['E05.318.308', 'L01.399.250', 'N05.715.360.300', 'N06.850.520.308'], ['H02.403.250'], ['M01.526.702.250'], ['I02.358.337.350.500', 'I02.358.399.350.750']]
|
['Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Information Science [L]', 'Disciplines and Occupations [H]', 'Named Groups [M]', 'Anthropology, Education, Sociology, and Social Phenomena [I]']
| 0
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 1
| 1
| 1
| 1
|
Hepatic dysfunction following cardiac surgery: determinants and consequences.
|
BACKGROUND/AIMS: We prospectively studied the determinants, characteristics, and consequences of hepatic dysfunction in the early postoperative period following cardiac surgery.METHODOLOGY: We examined 3041 adult patients, mean age 60.6 (+/- 8.9), with normal pre-operative liver function who consecutively underwent open heart surgery in a newly established Cardiac Surgery Center. Patients were divided into two groups; Group A included all patients who developed hepatic dysfunction, defined as the presence of jaundice associated with an elevated serum bilirubin above 3 mg/dl, in the early postoperative period. The control group included cardiac surgical patients who did not develop such dysfunction.RESULTS: Hepatic dysfunction developed in 96 patients (3.2%). The affected patients consisted of 63 males and 33 females, mean age 60.8 (+/- 9.4). Determinants of hepatic dysfunction based on univariate analysis were sex, NYHA class, type of surgery, operative times, low cardiac output syndrome necessitating administration of inotropic agents and/or IABP usage, cardiac arrest, presence of hematomas, and number of blood transfusions. Patients with hepatic dysfunction required prolonged mechanical ventilation, stayed longer in the ICU (and in the hospital) and experienced a much higher mortality rate (11.4%) compared to the control group (p = 0.001).CONCLUSION: Although the pathogenesis of hepatic dysfunction seems to be multifactorial, liver cell damage due to decreased perioperative hepatic flow and increased bilirubin load seem to be of critical importance. Early postoperative hepatic dysfunction resulted in increased morbidity and mortality.
|
['Adolescent', 'Adult', 'Aged', 'Aged, 80 and over', 'Cardiac Surgical Procedures', 'Female', 'Humans', 'Jaundice', 'Liver Diseases', 'Liver Function Tests', 'Male', 'Middle Aged']
| 9,222,689
|
[['M01.060.057'], ['M01.060.116'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['E04.100.376', 'E04.928.220'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C23.550.429.500', 'C23.888.885.375'], ['C06.552'], ['E01.370.372.460'], ['M01.060.116.630']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
IL-6 and IL-1 beta in fever. Studies using cytokine-deficient (knockout) mice.
|
Previous data support the hypothesis that during inflammation, interleukin (IL)-1 beta and IL-6 are involved in fever, in activation of the hypothalamic-pituitary-adrenal (HPA) axis, and in the induction of eicosanoids. Most of the pathophysiologic effects of IL-1 beta and Il-6 are mediated by prostaglandins (PGs), modulated by other cytokines, and antagonized by glucocorticoids (GC), a final product of the HPA axis. To further test these relationships, we measured changes in body temperature using biotelemetry in mice deficient in genes for IL-1 beta and/or IL-6 (IL-1 beta knockout [KO] and IL-6 KO) following injection with lipopolysaccharide (LPS) to induce systemic inflammation or turpentine to induce local abscess. Circulating IL-6, tumor necrosis factor alpha (TNF-alpha), GC, and PGE2 were measured in these mice after treatment. IL-1 beta KO mice responded with reduced fever and IL-6 KO mice with normal fever to a high dose of LPS. In contrast, neither type of KO mice produced fever to turpentine. PGE2 levels (measured in the circulation) were suppressed in both types of KO mice injected with turpentine. IL-1 beta KO mice showed deficiency in IL-6 following turpentine, but not LPS, injection. LPS-induced increases in TNF-alpha did not differ between IL-1 beta KO mice and their wild-type counterparts, whereas IL-6 KO mice showed exacerbated LPS-induced circulating TNF-alpha. No differences were noted in plasma elevations of GC between KO and wild-type mice following injection of LPS or turpentine, indicating that IL-1 beta and IL-6 are not required for activation of the HPA axis during inflammation. Our data demonstrate that in the mouse, IL-1 beta and IL-6 are critical for the induction of fever during local inflammation, whereas in systemic inflammation they appear only to contribute to fever.
|
['Animals', 'Chimera', 'Dinoprostone', 'Escherichia coli', 'Female', 'Fever', 'Inflammation', 'Interleukin-1', 'Interleukin-6', 'Lipopolysaccharides', 'Male', 'Mice', 'Mice, Knockout', 'Time Factors', 'Tumor Necrosis Factor-alpha', 'Turpentine']
| 9,917,862
|
[['B01.050'], ['B05.200'], ['D10.251.355.255.550.250.200', 'D23.469.050.175.725.250.200'], ['B03.440.450.425.325.300', 'B03.660.250.150.180.100'], ['C23.888.119.344'], ['C23.550.470'], ['D12.644.276.374.465.010', 'D12.644.276.374.500.400', 'D12.776.467.374.465.010', 'D12.776.467.374.500.400', 'D23.529.374.465.131', 'D23.529.374.500.400'], ['D12.644.276.374.465.224', 'D12.776.467.374.465.202', 'D23.529.374.465.224'], ['D09.400.500', 'D09.698.718.450', 'D10.494', 'D23.050.161.616.525', 'D23.946.123.329.500'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.136.500.500', 'B01.050.150.900.649.313.992.635.505.500.550.455', 'B01.050.150.900.649.313.992.635.505.500.800.500'], ['G01.910.857'], ['D12.644.276.374.500.800', 'D12.644.276.374.750.626', 'D12.776.124.900', 'D12.776.395.930', 'D12.776.467.374.500.800', 'D12.776.467.374.750.626', 'D23.529.374.500.800', 'D23.529.374.750.626'], ['D10.627.675.800', 'D20.215.721.500.881']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[Programs for the detection of gynecologic cancer].
|
The author reviews the problem of the early detection of breast cancer in women. He tells about the etiopathogenic complex, and the risk factors in relation to breast cancer, and indicates the protocol followed for breast cancer early detection. The usefulness of the employed method is supported by the obtained results after studying 69,635 women, and makes an economical evaluation of the cancer detection campaigns.
|
['Breast Diseases', 'Breast Neoplasms', 'Diagnosis, Differential', 'Female', 'Humans', 'Mammography', 'Mass Screening', 'National Health Programs', 'Palpation', 'Risk', 'Spain', 'Thermography']
| 6,546,175
|
[['C17.800.090'], ['C04.588.180', 'C17.800.090.500'], ['E01.171'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.350.700.500'], ['E01.370.500', 'E05.318.308.980.438.580', 'N02.421.726.233.443', 'N05.715.360.300.800.438.500', 'N06.850.520.308.980.438.580', 'N06.850.780.500'], ['N03.349.550'], ['E01.370.600.600'], ['E05.318.740.600.800', 'G17.680.750', 'N05.715.360.750.625.700', 'N06.850.520.830.600.800'], ['Z01.542.846'], ['E01.370.350.800', 'E05.933.500']]
|
['Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Geographicals [Z]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
|
Therapeutic value of scapular and pelvic girdle disarticulations in sarcoma.
|
The authors have reviewed 22 cases of proximal disarticulations with the aim of assessing the therapeutic value, taking into account previous radio- and chemotherapy. The following criteria were especially examined: recurrences, survival, quality of life. There were 13/22 soft tissue sarcomas, 9/22 bone sarcomas. In 10 instances, the tumour was primary and treated for the first time whilst, in 12 cases, it was recurrence. Eighteen patients had been previously treated by non radical surgery, 11 by radiotherapy and 10 by chemotherapy. For upper limb tumours, six patients underwent an inter-scapulo-thoracic disarticulation and three an inter-scapulo-thoracic resection according to Tykhor-Lindberg. For lower limb tumours, seven patients were submitted to inter-ilio-abdominal disarticulation, three to coxo-femoral disarticulation and one to internal hemipelvectomy according to Eilber. Mean disease free interval has been 34.5 months and mean survival 38.5 months. Three out of 20 evaluable patients (15%) recurred locally although most of them benefited from second surgery. Quality of life has been excellent in general despite the fact that only seven patients accepted wearing a prosthesis. Karnofsky index ranged between 60 and 100%. No significant difference was seen, whether or not previous radiotherapy and/or chemotherapy had been administered.
|
['Bone Neoplasms', 'Disarticulation', 'Evaluation Studies as Topic', 'Hemipelvectomy', 'Hip Joint', 'Humans', 'Sarcoma', 'Shoulder Joint', 'Soft Tissue Neoplasms']
| 3,595,886
|
[['C04.588.149', 'C05.116.231'], ['E04.555.080.250'], ['E05.337', 'N05.715.360.335'], ['E04.555.080.380'], ['A02.835.583.411'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C04.557.450.795'], ['A02.835.583.748'], ['C04.588.839']]
|
['Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Anatomy [A]', 'Organisms [B]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
MicroRNA loci support conspecificity of Gyrodactylus salaris and Gyrodactylus thymalli (Platyhelminthes: Monogenea).
|
The monogenean flatworm Gyrodactylus salaris is a serious threat to wild and farmed Atlantic salmon stocks in Norway. Morphologically, the closely related but harmless Gyrodactylus thymalli on grayling can hardly be distinguished from G. salaris. Until now, molecular approaches could not resolve unambiguously whether G. salaris and G. thymalli represent just one polytypic species, two polytypic species or a complex of more than two species. In the first known genome-wide analysis utilizing 37 conserved microRNA loci, the genetic differentiation of seven populations of G. salaris and G. thymalli was assessed. The concatenated alignment spanned 21,742bp including 62 variable positions. A neighbor-joining cluster analysis did not support any host-based or mitochondrial haplotype-based grouping of strains. We conclude that a two species concept for G. salaris and G. thymalli does not reflect meaningful biological entities. Instead, G. salaris and G. thymalli are just one species comprising several pathogenic and non-pathogenic strains on various primary hosts. Following the International Code for Zoological Nomenclature, G. salaris Malmberg, 1957 is the valid species name with G. thymalli ?it?an, 1960 becoming the junior synonym. Accordingly, the range of G. salaris is significantly increased, given that formerly G. salaris-free countries such as e.g., Great Britain are now within the species' natural range. The synonymization of G. salaris and G. thymalli implies severe challenges to current disease management routines, which assume that G. salaris and G. thymalli are readily distinguishable. Protocols for reliable identification of pathogenic and non-pathogenic strains of G. salaris need to be developed.
|
['Animal Distribution', 'Animals', 'Genetic Variation', 'MicroRNAs', 'Molecular Sequence Data', 'Phylogeny', 'Polymerase Chain Reaction', 'RNA, Helminth', 'Species Specificity', 'Trematoda']
| 24,998,346
|
[['F01.145.113.069', 'G16.049'], ['B01.050'], ['G05.365'], ['D13.150.650.319', 'D13.444.735.150.319', 'D13.444.735.790.552.500'], ['L01.453.245.667'], ['G05.697', 'G16.075.605', 'L01.100.697'], ['E05.393.620.500'], ['D13.444.735.520'], ['G16.824'], ['B01.050.500.500.736.715']]
|
['Psychiatry and Psychology [F]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Soil organic carbon and total nitrogen stocks in alpine ecosystems of Altun Mountain National Nature Reserve in dry China.
|
The Altun Mountain National Nature Reserve (AMNNR), characterized by complex topography, is located on the northern edge of the Qinghai-Tibetan Plateau. The stocks of soil organic carbon (SOC) and total nitrogen (TN) are critically important for carbon and nitrogen sequestration in dry alpine ecosystems of the AMNNR, which is a "natural laboratory" for assessing the carbon and nitrogen storage without human disturbance. We explored the stocks of SOC and TN in soils of different dry alpine ecosystems by sampling 23 sites across the AMNNR during 2013. The results showed that the SOC and TN stocks of AMNNR varied significantly with ecosystem types. The SOC stocks of 0-15 cm were highest in the alpine wet meadow (7.96 kg/m2), followed by alpine steppe (2.63 kg/m2). The stocks of SOC and TN in 0-5 and 5-10 cm soils of alpine wet meadow were significantly (P < 0.05) higher than those in the soils of other dry alpine ecosystems. In the whole AMNNR, total storage of SOC and TN were approximately 80.97 and 4.48 Tg, 34.25% of SOC and 24.01% of TN were stored in the alpine steppe, 21.51% of SOC and 26.01% of TN were stored in the alpine scrub, the largest ecosystem in the AMNNR. Our findings suggested it is important to protect the soil and vegetation of the dry alpine ecosystems, particularly the alpine wet meadow and alpine scrub to promote the carbon storage.
|
['Carbon', 'China', 'Conservation of Natural Resources', 'Ecosystem', 'Environmental Monitoring', 'Nitrogen', 'Plants', 'Soil']
| 30,593,592
|
[['D01.268.150'], ['Z01.252.474.164'], ['J01.256', 'N06.230.080'], ['G16.500.275.157', 'N06.230.124'], ['N06.850.460.350.080', 'N06.850.780.375'], ['D01.268.604', 'D01.362.625'], ['B01.650'], ['D20.721', 'G01.311.820', 'G16.500.275.815', 'N06.230.600']]
|
['Chemicals and Drugs [D]', 'Geographicals [Z]', 'Technology, Industry, and Agriculture [J]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
|
Identification of novel immunogenic proteins of Helicobacter pylori by proteome technology.
|
Cell surface proteins of the human gastric pathogen Helicobacter pylori, reference strain CCUG 17874, were extracted with acid glycine and fractionated by heparin affinity chromatography. The extracts were subsequently analysed using high-resolution two-dimensional gel electrophoresis (2-DE) and immunoblotting. Four proteins of low molecular masses (25-30 kDa) stained by Coomassie R-350, were identified by peptide ESI-MS/MS sequencing after in-gel tryptic digestion. The identified proteins were recognised by sera from H. pylori-infected patients. Two of them are now described for the first time as immunogenic proteins of which one protein was determined to be distinct from all H. pylori proteins previously described. In addition, the specificity of the identified peptides was evaluated using both 1-D and 2-D immunoblotting against a panel of sera from patients with various bacterial infections. The present identification of highly specific antigens of H. pylori will encourage the improvement of serological diagnostic tests to diagnose and monitor H. pylori infection.
|
['Antigens, Bacterial', 'Bacterial Outer Membrane Proteins', 'Cross Reactions', 'Electrophoresis, Gel, Two-Dimensional', 'Helicobacter Infections', 'Helicobacter pylori', 'Humans', 'Immunoblotting', 'Proteome', 'Serologic Tests']
| 11,730,836
|
[['D23.050.161'], ['D12.776.097.120', 'D12.776.543.100'], ['G12.122.281'], ['E05.196.401.250', 'E05.301.300.230'], ['C01.150.252.400.466'], ['B03.440.500.550', 'B03.660.150.235.500.250.550'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.478.566.320', 'E05.601.470.320'], ['D12.776.817'], ['E01.370.225.812.735', 'E05.200.812.735', 'E05.478.594.760']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[Intraabdominal metastasis of cerebellar medulloblastoma through ventriculoperitoneal shunt].
|
We present a 6-year-old girl with cerebellar medulloblastoma causing obstructive hydrocephalus that was treated by ventriculoperitoneal shunting. The patient subsequently underwent surgical excision of the tumor followed by adjuvant craniospinal radiotherapy. Nine months after shunting, multiple intraabdominal metastatic lesions were found. Although the risk is low, ventriculoperitoneal shunting may facilitate the spread of malignant cells.
|
['Abdominal Neoplasms', 'Cerebellar Neoplasms', 'Child', 'Female', 'Humans', 'Medulloblastoma', 'Neoplasm Seeding', 'Ventriculoperitoneal Shunt']
| 11,181,202
|
[['C04.588.033'], ['C04.588.614.250.195.411.211', 'C10.228.140.211.500.200', 'C10.228.140.252.200', 'C10.551.240.250.400.300'], ['M01.060.406'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C04.557.465.625.600.380.515', 'C04.557.465.625.600.590.500', 'C04.557.470.670.380.515', 'C04.557.470.670.590.500', 'C04.557.580.625.600.380.515', 'C04.557.580.625.600.590.500'], ['C04.697.650.830', 'C23.550.727.650.830'], ['E04.035.188.850', 'E04.525.170.850']]
|
['Diseases [C]', 'Named Groups [M]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Isolation of a gene (DLG3) encoding a second member of the discs-large family on chromosome 17q12-q21.
|
The discs-large family is a collection of proteins that have a common structural organization and are thought to be involved in signal transduction and mediating protein-protein interactions at the cytoplasmic surface of the cell membrane. The defining member of this group of proteins is the gene product of the Drosophila lethal (1) discs large (dlg) 1 locus, which was originally identified by the analysis of recessive lethal mutants. Germline mutations in dlg result in loss of apical-basolateral polarity, disruption of normal cell-cell adhesion, and neoplastic overgrowth of the imaginal disc epithelium. We have isolated and characterized a novel human gene, DLG3, that encodes a new member of the discs-large family of proteins. The putative DLG3 gene product has a molecular weight of 66 kDa and contains a discs-large homologous region, a src oncogene homology motif 3, and a domain with homology to guanylate kinase. The DLG3 gene is located on chromosome 17, in the same segment, 17q12-q21, as the related gene, DLG2. The products of the DLG2 and DLG3 genes show 36% identity and 58% similarity to each other, and both show nearly 60% sequence similarity to p55, an erythroid phosphoprotein that is a component of the red cell membrane. We suggest that p55, DLG2, and DLG3 are closely related members of a gene family, whose protein products have a common structural organization and probably a similar function.
|
['Amino Acid Sequence', 'Base Sequence', 'Chromosomes, Human, Pair 17', 'DNA, Complementary', 'Genome, Human', 'Humans', 'Insect Proteins', 'Molecular Sequence Data', 'Sequence Homology, Amino Acid', 'Tissue Distribution']
| 8,824,795
|
[['G02.111.570.060', 'L01.453.245.667.060'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['A11.284.187.520.300.415.425', 'G05.360.162.520.300.415.425'], ['D13.444.308.497.220', 'D13.444.600.223.500', 'D27.720.470.530.600.223.260'], ['G05.360.340.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.776.093.500'], ['L01.453.245.667'], ['G02.111.810.200', 'G05.810.200'], ['G03.787.917', 'G07.690.725.949']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Organisms [B]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Estradiol regulates angiopoietin-1 mRNA expression through estrogen receptor-alpha in a rodent experimental stroke model.
|
BACKGROUND AND PURPOSE: Female, compared with male, animals are protected from cerebral ischemic injury. Physiological concentrations of 17beta-estradiol (E2) reduce damage in experimental stroke. E2 augments angiogenesis in reproductive organs and noncerebral vascular beds. We hypothesized that E2 protects brain in stroke through modulation of angiogenesis. We quantified molecular markers of angiogenesis and capillary density before and after unilateral middle cerebral artery occlusion (MCAO).METHODS: Female animals were ovariectomized, treated with 25 microg E2 or placebo implants, and subjected to 2-hour MCAO and 22 hours of reperfusion. Brain angiopoietin-1 (Ang-1), Ang-2, Tie-1, Tie-2, vascular endothelial growth factor (VEGF), VEGF R1, and VEGF R2 mRNA levels were determined by RNAse protection assays, and CD31-positive vessels were counted.RESULTS: E2, but not ischemia, upregulated cerebral Ang-1 mRNA by 49%. Capillary density was higher in the brains of E2-treated animals. In estrogen receptor-alpha knockout (ERKO) mice, E2-mediated induction of Ang-1 mRNA was absent relative to wild-type littermates.CONCLUSIONS: These results suggest that E2 increases Ang-1 and enhances capillary density in brain under basal conditions, priming the MCA territory for survival after experimental focal ischemia.
|
['Angiopoietin-1', 'Animals', 'Brain', 'Capillaries', 'Disease Models, Animal', 'Estradiol', 'Estrogen Receptor alpha', 'Female', 'Gene Expression Regulation', 'Infarction, Middle Cerebral Artery', 'Mice', 'Mice, Knockout', 'Neovascularization, Pathologic', 'Placebos', 'Platelet Endothelial Cell Adhesion Molecule-1', 'RNA, Messenger', 'Rats', 'Rats, Wistar', 'Receptors, Estrogen', 'Reperfusion Injury', 'Ribonucleases', 'Stroke', 'Temperature', 'Time Factors', 'Transcription, Genetic', 'Up-Regulation', 'Vascular Endothelial Growth Factor A']
| 15,637,314
|
[['D12.644.276.100.100.100', 'D12.776.467.100.100.100', 'D23.529.100.100.100'], ['B01.050'], ['A08.186.211'], ['A07.015.461.165'], ['C22.232', 'E05.598.500', 'E05.599.395.080'], ['D04.210.500.365.415.248', 'D06.472.334.851.437.500'], ['D12.776.826.750.350.174'], ['G05.308'], ['C10.228.140.300.150.477.200.450', 'C10.228.140.300.510.200.387', 'C10.228.140.300.775.200.200.450', 'C14.907.253.092.477.200.450', 'C14.907.253.560.200.387', 'C14.907.253.855.200.200.450', 'C23.550.513.355.250.200.450', 'C23.550.717.489.250.200.450'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.136.500.500', 'B01.050.150.900.649.313.992.635.505.500.550.455', 'B01.050.150.900.649.313.992.635.505.500.800.500'], ['C23.550.589.500'], ['D26.660', 'E02.785'], ['D12.776.395.550.200.131', 'D12.776.543.550.200.131', 'D23.050.301.264.900.131', 'D23.050.301.350.131', 'D23.101.100.900.131'], ['D13.444.735.544'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.900'], ['D12.776.826.750.350', 'D12.776.930.778.350'], ['C14.907.725', 'C23.550.767.877'], ['D08.811.277.352.700'], ['C10.228.140.300.775', 'C14.907.253.855'], ['G01.906.595', 'G16.500.275.063.725.710', 'G16.500.750.775.710', 'N06.230.150.450', 'N06.230.300.100.725.710'], ['G01.910.857'], ['G02.111.873', 'G05.297.700'], ['G02.111.905', 'G05.308.850', 'G07.690.773.998'], ['D12.644.276.100.800.200', 'D12.776.467.100.800.200', 'D23.529.100.800.200']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Anatomy [A]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
A phase transition in energy-filtered RNA secondary structures.
|
In this article we study the effect of energy parameters on minimum free energy (mfe) RNA secondary structures. Employing a simplified combinatorial energy model that is only dependent on the diagram representation and is not sequence-specific, we prove the following dichotomy result. Mfe structures derived via the Turner energy parameters contain only finitely many complex irreducible substructures, and just minor parameter changes produce a class of mfe structures that contain a large number of small irreducibles. We localize the exact point at which the distribution of irreducibles experiences this phase transition from a discrete limit to a central limit distribution and, subsequently, put our result into the context of quantifying the effect of sparsification of the folding of these respective mfe structures. We show that the sparsification of realistic mfe structures leads to a constant time and space reduction, and that the sparsification of the folding of structures with modified parameters leads to a linear time and space reduction. We, furthermore, identify the limit distribution at the phase transition as a Rayleigh distribution.
|
['Nucleic Acid Conformation', 'RNA', 'Sequence Analysis, RNA']
| 23,057,821
|
[['G02.111.570.820.486', 'G05.360.580'], ['D13.444.735'], ['E05.393.760.710']]
|
['Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
From plan to action.
|
A strategic plan is your organizational touchstone Given the rapid rate of change in health care, your practice's capacity to succeed is based on its ability to adapt quickly; this, in turn, relies on the level of detail in the operational plan. A well-devised and detailed plan will enable your practice to navigate the waters of industry change. An operational plan begins with the practice's vision, values and mission.
|
['Organizational Objectives', 'Planning Techniques', 'Practice Management, Medical', 'United States']
| 16,900,904
|
[['N04.452.615'], ['N04.452.718'], ['N04.452.758.708.450'], ['Z01.107.567.875']]
|
['Health Care [N]', 'Geographicals [Z]']
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
|
Determination of myeloperoxidase-induced apoAI-apoAII heterodimers in high-density lipoprotein.
|
Myeloperoxidase secreted by macrophages and neutrophils in atherosclerotic lesions generates a tyrosyl radical in apolipoprotein (apo) AI, a major protein component of high-density lipoprotein (HDL), thus inducing the formation of apoAI-apoAII heterodimers. It can also cause nitration and chlorination of tyrosine residues. Determining the apoAI-apoAII heterodimer could provide useful information as to functional changes in HDL and/or the progression of atherosclerotic lesions. To this end, the apoAI-apoAII heterodimer was identified in normal human serum by immunoblotting; the band intensity was increased by treatment with myeloperoxidase. This apparent increase in heterodimer formation was quantitatively confirmed by ELISA. In normal human serum, a significant correlation between the concentrations of apoAI-apoAII heterodimer and free apoAII (r=0.763), but not free apoAI (r=0.093), was observed, indicating that heterodimer formation is likely induced on HDL particles carrying both apoAI and apoAII (Lp-AI/AII). In preliminary studies, the levels of apoAI-apoAII heterodimer were statistically higher in plasma from subjects with acute myocardial infarction (AMI) as compared to controls. These findings indicate the possibility that the apoAI-apoAII heterodimer, including nitration and chlorination modifications, may serve as an indicator of atherosclerotic lesions.
|
['Apolipoprotein A-I', 'Apolipoprotein A-II', 'Atherosclerosis', 'Chromatography, High Pressure Liquid', 'Dimerization', 'Enzyme-Linked Immunosorbent Assay', 'Humans', 'Immunoblotting', 'Lipoproteins, HDL', 'Myocardial Infarction', 'Peroxidase']
| 23,090,734
|
[['D10.532.091.200.100', 'D12.776.070.400.200.100', 'D12.776.521.120.200.100'], ['D10.532.091.200.150', 'D12.776.070.400.200.150', 'D12.776.521.120.200.150'], ['C14.907.137.126.307'], ['E05.196.181.400.300'], ['G02.206', 'G03.230'], ['E05.478.566.350.170', 'E05.478.566.380.360', 'E05.478.583.400.170', 'E05.601.470.350.170', 'E05.601.470.380.360'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.478.566.320', 'E05.601.470.320'], ['D10.532.432', 'D12.776.521.479'], ['C14.280.647.500', 'C14.907.585.500', 'C23.550.513.355.750', 'C23.550.717.489.750'], ['D08.811.682.732.700']]
|
['Chemicals and Drugs [D]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Glucose induces an autocrine activation of the Wnt/beta-catenin pathway in macrophage cell lines.
|
The canonical Wnt signalling pathway acts by slowing the rate of ubiquitin-mediated beta-catenin degradation. This results in the accumulation and subsequent nuclear translocation of beta-catenin, which induces the expression of a number of genes involved in growth, differentiation and metabolism. The mechanisms regulating the Wnt signalling pathway in the physiological context is still not fully understood. In the present study we provide evidence that changes in glucose levels within the physiological range can acutely regulate the levels of beta-catenin in two macrophage cell lines (J774.2 and RAW264.7 cells). In particular we find that glucose induces these effects by promoting an autocrine activation of Wnt signalling that is mediated by the hexosamine pathway and changes in N-linked glycosylation of proteins. These studies reveal that the Wnt/beta-catenin system is a glucose-responsive signalling system and as such is likely to play a role in pathways involved in sensing changes in metabolic status.
|
['Animals', 'Axin Protein', 'Carcinoma, Hepatocellular', 'Cell Line, Tumor', 'Cyclin D1', 'Cytoskeletal Proteins', 'DNA Primers', 'Gene Expression Regulation, Neoplastic', 'Glucose', 'Homeostasis', 'Humans', 'Liver Neoplasms', 'Macrophages', 'Mice', 'RNA', 'Wnt Proteins', 'beta Catenin']
| 18,823,284
|
[['B01.050'], ['D05.500.117.500', 'D12.776.476.081.500'], ['C04.557.470.200.025.255', 'C04.588.274.623.160', 'C06.301.623.160', 'C06.552.697.160'], ['A11.251.210.190', 'A11.251.860.180'], ['D12.644.360.262.150.100', 'D12.776.167.218.150.100', 'D12.776.476.262.150.100', 'D12.776.624.664.700.100'], ['D12.776.220'], ['D13.695.578.424.450.275', 'D27.720.470.530.600.223.600'], ['G05.308.370'], ['D09.947.875.359.448'], ['G07.410'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C04.588.274.623', 'C06.301.623', 'C06.552.697'], ['A11.329.372', 'A11.627.482', 'A11.733.397', 'A15.382.670.522', 'A15.382.680.397'], ['B01.050.150.900.649.313.992.635.505.500'], ['D13.444.735'], ['D12.776.467.984', 'D23.529.984'], ['D12.776.091.249', 'D12.776.220.145.500', 'D12.776.930.130']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Anatomy [A]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Use of augmented sensory feedback to achieve symmetrical standing.
|
Ambulatory patients with hemiparesis were given auditory feedback of weight bearing on the involved leg in order to achieve symmetrical standing. A device for augmented sensory feedback, the Limb Load Monitor, was used for the training. Patients who could correct their limb loading pattern during the first treatment session learned to achieve symmetrical standing. Results are also related to the magnitude of weight bearing on the involved limb and the side on which the lesion occurs. These findings suggest that the patients utilized the feedback signal to control an inherent activity.
|
['Adult', 'Aged', 'Biofeedback, Psychology', 'Female', 'Follow-Up Studies', 'Hemiplegia', 'Humans', 'Male', 'Middle Aged', 'Posture', 'Sound']
| 643,934
|
[['M01.060.116'], ['M01.060.116.100'], ['E02.190.525.123', 'F02.830.131', 'F04.754.137.301', 'F04.754.308.500'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['C10.597.622.295', 'C23.888.592.636.312'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['G11.427.695'], ['G01.750.770.776']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Psychiatry and Psychology [F]', 'Health Care [N]', 'Diseases [C]', 'Organisms [B]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Residue-level prediction of DNA-binding sites and its application on DNA-binding protein predictions.
|
Protein-DNA interactions are crucial to many cellular activities such as expression-control and DNA-repair. These interactions between amino acids and nucleotides are highly specific and any aberrance at the binding site can render the interaction completely incompetent. In this study, we have three aims focusing on DNA-binding residues on the protein surface: to develop an automated approach for fast and reliable recognition of DNA-binding sites; to improve the prediction by distance-dependent refinement; use these predictions to identify DNA-binding proteins. We use a support vector machines (SVM)-based approach to harness the features of the DNA-binding residues to distinguish them from non-binding residues. Features used for distinction include the residue's identity, charge, solvent accessibility, average potential, the secondary structure it is embedded in, neighboring residues, and location in a cationic patch. These features collected from 50 proteins are used to train SVM. Testing is then performed on another set of 37 proteins, much larger than any testing set used in previous studies. The testing set has no more than 20% sequence identity not only among its pairs, but also with the proteins in the training set, thus removing any undesired redundancy due to homology. This set also has proteins with an unseen DNA-binding structural class not present in the training set. With the above features, an accuracy of 66% with balanced sensitivity and specificity is achieved without relying on homology or evolutionary information. We then develop a post-processing scheme to improve the prediction using the relative location of the predicted residues. Balanced success is then achieved with average sensitivity, specificity and accuracy pegged at 71.3%, 69.3% and 70.5%, respectively. Average net prediction is also around 70%. Finally, we show that the number of predicted DNA-binding residues can be used to differentiate DNA-binding proteins from non-DNA-binding proteins with an accuracy of 78%. Results presented here demonstrate that machine-learning can be applied to automated identification of DNA-binding residues and that the success rate can be ameliorated as more features are added. Such functional site prediction protocols can be useful in guiding consequent works such as site-directed mutagenesis and macromolecular docking.
|
['Amino Acids', 'Artificial Intelligence', 'Binding Sites', 'DNA', 'DNA-Binding Proteins', 'Macromolecular Substances', 'Models, Biological', 'Models, Molecular', 'Protein Binding']
| 17,316,627
|
[['D12.125'], ['G17.035.250', 'L01.224.050.375'], ['G02.111.570.120'], ['D13.444.308'], ['D12.776.260'], ['D05'], ['E05.599.395'], ['E05.599.595'], ['G02.111.679', 'G03.808']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Heterotrimeric G protein betagamma subunits stimulate FLJ00018, a guanine nucleotide exchange factor for Rac1 and Cdc42.
|
We previously reported that Gbetagamma signaling regulates cell spreading or cell shape change through activation of a Rho family small GTPase, suggesting the existence of a Gbetagamma-regulated Rho guanine-nucleotide exchange factor (RhoGEF). In this study we examined various RhoGEF clones, found FLJ00018 to beaGbetagamma-activated RhoGEF, and investigated the molecular mechanism of Gbetagamma-induced activation of Rho family GTPases. Co-expression of the genes for FLJ00018 and Gbetagamma enhanced serum response element-mediated gene transcription in HEK-293 cells. Combined expression of Gbetagamma and FLJ00018 significantly induced activation of Rac and Cdc42 but not RhoA. FLJ00018 also enhanced gene transcription induced by carbachol-stimulated m2 muscarinic acetylcholine receptor, and this enhancement was blocked by pertussis toxin. Furthermore, we demonstrated Gbetagamma to interact directly with the N-terminal region of FLJ00018 and the N-terminal fragment of this molecule to inhibit serum response element-dependent transcription induced by Gbetagamma/FLJ00018 and carbachol. In NIH3T3 cells, FLJ00018 enhanced lysophosphatidic acid-induced cell spreading, which was also blocked by the N-terminal fragment of FLJ00018. These results provide evidence for a signaling pathway by which G(i)-coupled receptor specifically induces Rac and Cdc42 activation through direct interaction of Gbetagamma with FLJ00018.
|
['Animals', 'Carbachol', 'Cell Shape', 'Cholinergic Agonists', 'Enzyme Activation', 'Gene Expression', 'Guanine Nucleotide Exchange Factors', 'Heterotrimeric GTP-Binding Proteins', 'Humans', 'Lysophospholipids', 'Mice', 'NIH 3T3 Cells', 'Neuropeptides', 'Pertussis Toxin', 'Receptor, Muscarinic M2', 'Serum Response Element', 'Signal Transduction', 'Transcription, Genetic', 'cdc42 GTP-Binding Protein', 'rac GTP-Binding Proteins', 'rac1 GTP-Binding Protein', 'rho GTP-Binding Proteins', 'rhoA GTP-Binding Protein']
| 18,045,877
|
[['B01.050'], ['D02.092.877.883.333.115', 'D02.675.276.232.115'], ['G04.320'], ['D27.505.519.625.120.140', 'D27.505.696.577.120.140'], ['G02.111.263', 'G03.328'], ['G05.297'], ['D12.644.360.325.300', 'D12.776.476.325.300'], ['D08.811.277.040.330.300.200', 'D12.644.360.360', 'D12.776.157.325.332', 'D12.776.476.375', 'D12.776.543.325'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D10.570.755.375.760.550'], ['B01.050.150.900.649.313.992.635.505.500'], ['A11.251.210.100.550', 'A11.329.228.100.550'], ['D12.644.400', 'D12.776.631.650'], ['D08.811.913.400.725.115.680', 'D23.946.123.946.690', 'D23.946.896.980.690'], ['D12.776.543.750.695.475.200', 'D12.776.543.750.720.360.500.200'], ['G02.111.570.080.689.330.700.800', 'G02.111.570.080.689.675.700.800', 'G05.360.080.689.330.700.800', 'G05.360.080.689.675.700.800', 'G05.360.340.024.340.137.750.249.765.800', 'G05.360.340.024.340.137.750.680.765.800'], ['G02.111.820', 'G04.835'], ['G02.111.873', 'G05.297.700'], ['D08.811.277.040.330.300.400.700.050', 'D12.644.360.525.700.050', 'D12.776.157.325.515.700.050', 'D12.776.476.525.700.050'], ['D08.811.277.040.330.300.400.700.100', 'D12.644.360.525.700.100', 'D12.776.157.325.515.700.100', 'D12.776.476.525.700.100'], ['D08.811.277.040.330.300.400.700.100.500', 'D12.644.360.525.700.100.100', 'D12.776.157.325.515.700.100.100', 'D12.776.476.525.700.100.100'], ['D08.811.277.040.330.300.400.700', 'D12.644.360.525.700', 'D12.776.157.325.515.700', 'D12.776.476.525.700'], ['D08.811.277.040.330.300.400.700.200', 'D12.644.360.525.700.200', 'D12.776.157.325.515.700.200', 'D12.776.476.525.700.200']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
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